CN111150670B

CN111150670B - Anti-ultraviolet and anti-blue-light liquid crystal nano-lipid liquid and preparation method thereof

Info

Publication number: CN111150670B
Application number: CN202010039571.1A
Authority: CN
Inventors: 张雷; 杨淑琴; 郭秀茹; 冯晨; 王旭研
Original assignee: Beijing Belilles Biotechnology Co ltd
Current assignee: Beijing Belilles Biotechnology Co ltd
Priority date: 2020-01-15
Filing date: 2020-01-15
Publication date: 2022-09-23
Anticipated expiration: 2040-01-15
Also published as: CN111150670A

Abstract

The invention provides an anti-ultraviolet and anti-blue liquid crystal nano-liposome liquid, which comprises the following components: based on the total weight of the liquid crystal nano-lipid liquid, 1-10 wt% of liquid crystal emulsifier, 5-15 wt% of grease, 2-20 wt% of anti-ultraviolet and anti-blue light composition, 60-86 wt% of water, and the sum of the components is one hundred percent; wherein the anti-ultraviolet and anti-blue light composition at least comprises any three of troxerutin, ferulic acid, resveratrol and baicalin; preferably, the composition comprises troxerutin, ferulic acid, resveratrol, baicalin; preferably, the content of troxerutin is 9-40 wt% based on the total weight of the composition; the content of ferulic acid is 5-30 wt%; the resveratrol content is 0.8-20 wt%; the content of baicalin is 0.8-10 wt%; wherein, the sum of the components of the composition is one hundred percent. The liquid crystal nano-lipid liquid can resist the harm of ultraviolet rays and blue light in all wave bands, and the biological absorptivity and the moisture retention performance of the product are improved.

Description

Anti-ultraviolet and anti-blue-light liquid crystal nano-lipid liquid and preparation method thereof

Technical Field

The invention relates to the technical field of cosmetics, and relates to an anti-ultraviolet and anti-blue-light liquid crystal nano-liposome liquid and a preparation method thereof.

Technical Field

The liquid crystal is a state between solid and liquid, and is in a thermodynamically stable phase state between crystalline states, and is in an incompletely dissolved state, and has certain thermodynamic stability. The lamellar liquid crystal structure formed by the lecithin liquid crystal emulsifier can simulate human skin, is equivalent to the 'second layer skin' of a human body when being attached to the surface of the skin and has excellent barrier function; according to the principle of similarity and compatibility, the liquid crystal nano-lipid structure can promote the permeation of active substances on the surface layer of the skin; the liquid crystal phase can be adsorbed on an oil-water interface to form a stable protective layer, so that the condensation of liquid drops due to mutual collision can be prevented, and the stability of the system is improved; the multilayer structure of the lamellar liquid crystal can control the effect of slow release of the active ingredient and reduce the transfer speed of the active ingredient dissolved in oil drops among interfaces, thereby prolonging the activity of the active ingredient and controlling the slow release effect of the medicine; the water phase of the liquid crystal structure has a rubber net structure, the lamellar liquid crystal phase exists around the oil drops, the lamellar liquid crystal phase is of a multilayer structure absorbing and swelling water, and when the product with the structure is smeared on the skin, the water cannot be volatilized immediately, so that the effect of filling and lasting skin water can be achieved, and the moisturizing performance of the product is enhanced.

Ultraviolet rays are classified into three types according to the wavelength of sunlight: UVA, the longest wavelength of ultraviolet light, with a wavelength of 320-400nm, penetrates the dermis and penetrates deeper into the skin than UVB, tanning the skin and causing damage to lipids and collagen, causing photoaging of the skin and even skin cancer. UVB, mid-wave ultraviolet, wavelength 290- & lt320 nm, & gt, can reach the epidermis layer, causing the phenomena of skin sunburn, erythema, suntan and the like, and causing the greatest damage (visible damage) to the upper layer of the skin. UVC, wavelength 200 ~ 290nm, UVC can be absorbed by the atmosphere almost completely, and we can meet only rarely. When the skin is subjected to excessive exposure to ultraviolet rays, epidermal cells can be damaged; activating tyrosinase, accelerating pigment synthesis, destroying skin moisturizing function, drying skin, damaging elastic fiber in dermis layer, generating fine lines, and causing skin inflammation and burn under strong irradiation. In the case of an abnormal condition, skin cancer or the like with pigment property is caused.

Blue light, with a wavelength of 400nm to 480nm, has a relatively high energy, causes pigmentation of the skin for up to 3 months, and is particularly prone to pigmentation in the epidermal granular layer and deeper skin layers. Can penetrate deeper into the skin to cause oxidative damage to cellular DNA. Studies have now demonstrated that blue light alters the structure of epidermal cells and also reduces collagen and elastin production. The blue light exists in LED lamps, computer displays (including CCFL and LED), mobile phones, digital products, liquid crystal display screens, bath heaters and other light rays in a large amount, and the harm to human bodies is gradually increased.

Current products for protection against sunlight are mainly chemically synthesized organic sunscreens and physical sunscreens. Most of the chemical sunscreen agents play a role in sunscreen, and can cause damage to skin due to degradation. Some components have poor product compatibility, and are easy to cause stability problems when used in cosmetics. The physical sun-screening agent is generally used for shielding ultraviolet rays, and is mostly nano titanium dioxide and nano zinc dioxide, and the particle size of the particle is small, so that the particle is easy to enter skin pores and is not easy to clean.

The plant product has the characteristics of pure nature and safety. In recent years, the protection of plant products from sunlight is increasingly studied. However, at present, plant products with sunlight protection function in the market are relatively lacked, and the plant products only have the protection function on a single waveband, so that the protection effect is not ideal.

In addition, blue light protection is also needed for people with short outdoor activities and long-term indoor work, especially for people who adopt computers to work for a long time. At present, no such products, in particular ultraviolet-resistant and blue-light-resistant cosmetics with good moisturizing function exist in the market.

Disclosure of Invention

The invention aims to provide the liquid crystal nano lipid liquid capable of resisting ultraviolet rays and blue light aiming at the damage of the ultraviolet rays and the blue light to the skin, the liquid crystal nano lipid liquid can resist the harm of the ultraviolet rays and the blue light in all bands, the liquid crystal nano lipid structure can protect the stability of functional components, simulate the structure of the stratum corneum of the skin, improve the biological absorptivity of a product and enhance the moisturizing performance of the product.

The invention provides an anti-ultraviolet and anti-blue-light liquid crystal nano-liposome liquid, which is characterized by comprising the following components: based on the total weight of the liquid crystal nano-lipid liquid, 1-10 wt% of liquid crystal emulsifier, 5-15 wt% of grease, 2-20 wt% of ultraviolet-resistant and blue-resistant composition and 60-86 wt% of water, wherein the sum of the components is one hundred percent; wherein the anti-ultraviolet and anti-blue light composition at least comprises any three of troxerutin, ferulic acid, resveratrol and baicalin; preferably, the composition comprises troxerutin, ferulic acid, resveratrol, baicalin; preferably, the content of the troxerutin is 9-40 wt%, preferably 14-30 wt%, based on the total weight of the composition; the content of the ferulic acid is 5-30 wt%, preferably 6-20 wt%; the content of the resveratrol is 0.8-20 wt%, preferably 2-10 wt%; the content of baicalin is 0.8-10 wt%, preferably 2-8 wt%; wherein, the sum of the components of the composition is one hundred percent.

The invention provides application of the ultraviolet-resistant and blue-resistant liquid crystal nano-liposome liquid in cosmetics.

The anti-ultraviolet and anti-blue-light liquid crystal nano-liposome liquid provided by the invention has the following beneficial effects:

1. the liquid crystal nanometer lipid liquid structure has multiple effects of protecting the stability of effective components and the barrier function of skin, controlling the slow release effect, improving the bioavailability and enhancing the moisture retention performance. In particular, in a preferred embodiment, the liquid crystal nano-liposome solution has a particle size distribution in the range of 80 to 500nm, which can promote the absorption of active ingredients and impart a characteristic smooth skin feel.

2. The liquid crystal nano-liposome liquid provided by the invention can resist ultraviolet rays and blue light in a large-range wave band (280-440nm wave band), and has a broad-spectrum protection effect; therefore, the damage of UVB, UVA and blue light to the skin can be resisted at the same time, and the requirement of most people on sunlight protection is met. The liquid crystal nano-liposome liquid provided by the invention not only can protect light, but also can eliminate inflammatory factors, inhibit allergic reaction, relieve and repair skin and resist oxidation; therefore, can promote the repair after sunburn and has the function of anti-aging.

3. The liquid crystal nano lipid liquid has simple production process, can be produced in large batch by using conventional emulsification homogenization and high-pressure homogenization equipment.

Drawings

FIG. 1 is a UV spectrum of the composition prepared in example 1.

FIG. 2 is a blue light transmittance spectrum of the composition prepared in example 1.

FIG. 3 is a graph showing the particle size detection of the liquid crystal nano-liposome solution prepared in example 1.

FIG. 4 is a polarized light microscope liquid crystal structure detection diagram of the liquid crystal nano lipid liquid prepared in example 1.

Fig. 5 is a graph showing a particle size detection of the liquid crystal nano-liposome solution prepared in example 2.

FIG. 6 polarized light microscope liquid crystal structure detection map of liquid crystal nano lipid liquid prepared in example 2.

Fig. 7 is a graph showing a particle size detection of the liquid crystal nano-liposome solution prepared in example 3.

FIG. 8 is a polarized light microscope liquid crystal structure detection diagram of the liquid crystal nano lipid liquid prepared in example 3.

Fig. 9 is a uv spectrum of the composition prepared in example 4.

Fig. 10 is a graph showing a particle size detection of the liquid crystal nano-liposome solution prepared in example 4.

FIG. 11 is a polarized light microscope liquid crystal structure detection diagram of the liquid crystal nano lipid liquid prepared in example 4.

Detailed Description

An anti-ultraviolet and anti-blue-light liquid crystal nano-liposome liquid, which is characterized by comprising the following components: based on the total weight of the liquid crystal nano-lipid liquid, 1-10 wt% of liquid crystal emulsifier, 5-15 wt% of grease, 2-20 wt% of anti-ultraviolet and anti-blue light composition, 60-86 wt% of water, and the sum of the components is one hundred percent; wherein the anti-ultraviolet and anti-blue light composition at least comprises any three of troxerutin, ferulic acid, resveratrol and baicalin.

In a preferred embodiment, the liquid crystal emulsifier is 1-5 wt%, the oil is 6-8 wt%, and the composition for resisting ultraviolet rays and blue light is 2-10 wt%.

The liquid crystal emulsifier can be any emulsifier in the field, and preferably, the liquid crystal emulsifier is lecithin and a group consisting of one or more of caprylic/capric triglyceride, behenyl alcohol, stearyl alcohol, PEG-20 phytosterol, cetyl alcohol and glyceryl stearate.

The grease can be synthetic grease and/or vegetable grease; preferably, the synthetic grease is one or more of isononyl isononanoate, caprylic/capric triglyceride and isooctyl palmitate; the vegetable oil is one or more of rose fruit oil, sea buckthorn fruit oil, tea tree oil and wheat germ oil.

In a preferred embodiment, the composition comprises troxerutin, ferulic acid, resveratrol, baicalin; preferably, the content of the troxerutin is 9-40 wt%, preferably 14-30 wt%, based on the total weight of the composition; the content of the ferulic acid is 5-30 wt%, preferably 6-20 wt%; the content of the resveratrol is 0.8-20 wt%, preferably 2-10 wt%; the content of baicalin is 0.8-10 wt%, preferably 2-8 wt%; wherein, the sum of the components of the composition is one hundred percent.

Preferably, the content of said troxerutin in said composition may be from 12 to 40% by weight, preferably from 15 to 30% by weight; the ferulic acid may be present in the composition in an amount of 7-30 wt%, preferably 8-20 wt%; the resveratrol may be present in the composition in an amount of 1-20 wt%, preferably 2-10 wt%; the baicalin may be contained in the composition in an amount of 1-10 wt%, preferably 2-8 wt%.

The inventor finds that the composition has better ultraviolet resistance and blue light resistance.

In order to further enhance the anti-uv and anti-blue light effects, preferably, the composition comprises troxerutin, ferulic acid, resveratrol, baicalin; preferably, the content of the troxerutin is 9-40 wt%, preferably 14-30 wt%, based on the total weight of the composition; the content of the ferulic acid is 5-30 wt%, preferably 6-20 wt%; the content of the resveratrol is 0.8-20 wt%, preferably 2-10 wt%; the content of baicalin is 0.8-10 wt%, preferably 2-8 wt%; wherein the sum of all the components is one hundred percent.

In order to further enhance the anti-UV and anti-blue light effect, in a preferred embodiment, the composition further comprises one or more of quercetin, 4-hydroxy-3-butylphyllolide, emodin and baicalein.

In order to further enhance the anti-uv and anti-blue light effects, in a preferred embodiment, the composition further comprises quercetin, 4-hydroxy-3-butylphilin, emodin and baicalein; preferably, the content of quercetin is 1-10 wt% of the troxerutin, the content of 4-hydroxy-3-butyl-dense lactone is 1-10 wt% of the ferulic acid, the content of emodin is 1-10 wt% of the resveratrol, and the content of baicalein is 1-10 wt% of the baicalin.

In order to further enhance the anti-uv and anti-blue light effects, in a preferred embodiment, the troxerutin is derived from sophora flower bud extract, the ferulic acid is derived from ligusticum chuanxiong hort extract, the resveratrol is derived from polygonum cuspidatum extract, and the baicalin is derived from scutellaria baicalensis extract.

In one embodiment, the sophora flower bud extract is present in an amount of 10-45 wt%, preferably 15-30 wt%, based on the total weight of the composition; the content of the ligusticum chuanxiong hort extract is 7-35 wt%, and preferably 10-25 wt%; the content of the giant knotweed extract is 1-25%, preferably 2-20% by weight; the content of the scutellaria baicalensis extract is 1-15 wt%, preferably 2-10 wt%.

The flos Sophorae Immaturus extract mainly contains troxerutin (rutin), quercetin, etc. The flos Sophorae Immaturus extract has effects of scavenging free radicals, enhancing skin vessel wall elasticity and toughness, reducing permeability, relieving red blood streak, and resisting bacteria and inflammation.

The main components of rhizoma Ligustici Chuanxiong extract are ferulic acid, 4-hydroxy-3-butyl-concentrated lactone, etc. The rhizoma Ligustici Chuanxiong extract has antioxidant activity, can promote the generation of free radical scavenging enzyme, relieve radiation injury, inhibit tyrosinase activity, and has antibacterial and antiinflammatory effects.

The rhizoma Polygoni Cuspidati extract mainly contains resveratrol and emodin. The rhizoma Polygoni Cuspidati extract has effects of inhibiting lipid peroxidation, inhibiting melanin generation, promoting collagen synthesis, and resisting inflammation.

The Scutellariae radix extract mainly contains baicalin, baicalein, etc. The Scutellariae radix extract has effects in resisting inflammation and anaphylaxis, inhibiting bacteria, and scavenging free radicals.

The flos Sophorae Immaturus extract, rhizoma Ligustici Chuanxiong extract, rhizoma Polygoni Cuspidati extract, and Scutellariae radix extract can be obtained commercially.

In order to promote the dissolution of the ligusticum chuanxiong hort extract, the polygonum cuspidatum extract or the scutellaria baicalensis extract in the composition, in a preferred embodiment, the ligusticum chuanxiong hort extract, the polygonum cuspidatum extract or the scutellaria baicalensis extract is present in the form of microcapsules. Microcapsules of these extracts can be prepared according to the prior art, wherein the capsule material can be any material from which microcapsules are prepared, preferably hydroxypropyl cyclodextrin and/or chitosan.

In a preferred embodiment, the scutellaria baicalensis extract is in the form of microcapsule, and the capsule material is hydroxypropyl cyclodextrin and/or chitosan.

In a preferred embodiment, the microcapsule containing scutellaria baicalensis extract comprises the following ingredients: based on the total weight of the microcapsule containing the scutellaria baicalensis extract, the content of baicalin is 10-20 wt%, the content of capsule wall material is 80-90 wt%, the content of acid is 0.3-0.7 wt%, and the total weight of each component is one hundred percent. The capsule wall material can be hydroxypropyl cyclodextrin and chitosan; the acid may be organic and inorganic acids such as acetic acid, hydrochloric acid, sulfuric acid, phosphoric acid, and the like. In a preferred embodiment, the acid is arginine.

In a preferred embodiment, the microcapsule containing the scutellaria baicalensis extract is prepared by the following method: dispersing Scutellariae radix extract in solvent, adding acid to adjust pH to 6-7.5, preferably 6.5-7, heating to 45-60 deg.C, preferably 50-60 deg.C, adding capsule wall material, stirring to dissolve completely, cooling, filtering, and drying.

The solvent is various solvents that can dissolve the scutellaria baicalensis extract, but is preferably water, and more preferably deionized water.

The inventors found that the dissolution effect of the scutellaria baicalensis extract is very good when the pH of the solution is 6 to 7.5.

The temperature of cooling may be room temperature, but is preferably 5-10 deg.C, for example 6 deg.C.

The temperature for drying may be 50-80 deg.C, but is preferably 50-60 deg.C.

The capsule wall material can be any material for preparing microcapsules, preferably hydroxypropyl cyclodextrin and/or chitosan; preferably, the capsule wall material is hydroxypropyl cyclodextrin and/or chitosan.

The acid may be any organic and inorganic acid, such as acetic acid, hydrochloric acid, sulfuric acid, phosphoric acid, and the like. In a preferred embodiment, the acid is arginine.

In one embodiment, the composition further comprises a solvent; preferably, the composition is prepared by the steps of: heating solvent to 40-50 deg.C, adding flos Sophorae Immaturus extract, rhizoma Ligustici Chuanxiong extract and rhizoma Polygoni Cuspidati extract, stirring for 5-10 min, adding microcapsule containing Scutellariae radix extract, and stirring to dissolve completely.

In one embodiment, the composition comprises 10-17 wt% of sophora flower bud extract, 7-11 wt% of ligusticum chuanxiong hort extract, 1-5 wt% of polygonum cuspidatum extract, 10-22 wt% of microcapsule containing scutellaria baicalensis extract, 27-33 wt% of ethoxydiglycol, and 20-25 wt% of water.

The solvent may be any solvent used in cosmetics, and in a preferred embodiment, the solvent is ethoxydiglycol and water; when ethoxydiglycol and water are used as solvents, the sophora flower bud extract, the ligusticum chuanxiong hort extract, the polygonum cuspidatum extract and the microcapsule containing the scutellaria baicalensis extract are very well dissolved. In a preferred embodiment, the ethoxydiglycol is present in an amount of 25 to 35 wt.%, and the water is present in an amount of 20 to 25 wt.%, based on the total weight of the composition.

The composition may also contain antiseptic, which may be one or more of phenoxyethanol, ethyl hexyl glycerol, methyl paraben, p-hydroxyacetophenone, pentanediol, caprylyl glycol, hexanediol, etc. at any ratio. The preservative may be present in an amount of 0.1 to 1% by weight, for example, 0.5% by weight, based on the total weight of the composition.

In a preferred embodiment, the liquid crystal nano-liposome liquid further contains one or more of an antioxidant, a humectant and a preservative.

In a preferable embodiment, the liquid crystal nano lipid liquid further contains an antioxidant, a humectant and a preservative, wherein the liquid crystal emulsifier is 1-5 wt%, the oil and fat is 6-8 wt%, the ultraviolet and blue light resistant composition is 2-10 wt%, the antioxidant is 1-4 wt%, the humectant is 4-8 wt%, the preservative is 0.3-0.7 wt%, and the water is 60-86 wt%.

The antioxidant can be one or more of vitamin E acetate, pentaerythritol tetra (di-tert-butyl hydroxy hydrocinnamate), dibutyl hydroxy toluene and the like.

The humectant can be one or more of glycerol, sodium hyaluronate, 1, 2-propylene glycol, hydroxyethyl urea, sodium polyglutamate, trehalose, etc.

The antiseptic may be one or more of phenoxyethanol, ethyl hexyl glycerol, methyl paraben, p-hydroxyacetophenone, pentanediol, caprylyl glycol, hexanediol, etc.

Preferably, the preparation method of the liquid crystal nano-liposome liquid comprises the following steps:

(1) adding a liquid crystal emulsifier into water, and heating and dissolving to form a water phase;

(2) slowly adding the oil and fat into the water phase under homogenizing conditions, and homogenizing for a period of time, such as 3-8 minutes, such as 5 minutes;

(3) cooling the system of step (2) to 40-50 deg.C, adding the anti-UV and anti-blue light composition, and homogenizing for a period of time, e.g. 1-2 minutes, such as 1 minute;

(4) and (4) homogenizing the system in the step (3) under high pressure.

Preferably, the temperature in steps (1) and (2) is 70-90 ℃, e.g. 80 ℃.

When the liquid crystal nano-liposome liquid also contains a humectant, the humectant is added into the water phase in the step (1)

When the liquid crystal nano-liposome liquid also contains antioxidant, the antioxidant is added into the grease, and is heated to be dissolved, wherein the heating temperature is, for example, 60-70 ℃, such as 65 ℃.

When the liquid crystal nano lipid liquid further contains a preservative, the preservative is added in the step (3).

The invention also provides application of the ultraviolet-resistant and blue-resistant liquid crystal nano-liposome liquid in cosmetics.

The content of the anti-ultraviolet and anti-blue liquid crystal nano-liposome liquid in the cosmetic is 2-30 wt%.

The cosmetic may be a lotion, cream product. In the cosmetic, lecithin, glyceryl stearate, ceteareth-25 may be used as an emulsifier; isononyl isononanoate can be used as an oil; glycerin and hydroxyethyl urea can be used as moisturizers; tocopherol acetate can also be added as an antioxidant; an ammonium acryloyldimethyltaurate/VP copolymer may be used as the thickener.

The invention also provides an anti-ultraviolet and anti-blue-light cream, wherein the cream contains 2-30 wt% of anti-ultraviolet and anti-blue-light liquid crystal nano lipid liquid.

In order to facilitate a better understanding of the invention, reference is made to the following examples. These examples belong to the scope of protection of the present invention, but do not limit the scope of protection of the present invention.

Example 1

1. Preparing a microcapsule containing a scutellaria baicalensis extract: dispersing 15 g of scutellaria baicalensis extract powder (14 g of baicalin) into a proper amount of deionized water, adding 0.5 g of arginine to adjust the pH value of the solution to be 6.5, heating the solution to 60 ℃, adding 84.5 g of hydroxypropyl cyclodextrin, stirring until the hydroxypropyl cyclodextrin is completely dissolved, placing the mixture at 6 ℃ and cooling to obtain the microcapsule containing the scutellaria baicalensis extract. After filtration, the mixture was rotary evaporated at 60 ℃ to a dry powder.

2. Preparation of uv-and blue-light-resistant compositions: mixing 30 g of ethoxydiglycol and 23 g of water uniformly, heating to 45 ℃, keeping the temperature constant, stirring, slowly adding 15 g of sophora flower bud extract powder (14 g of troxerutin), 9 g of ligusticum chuanxiong hort extract powder (8 g of ferulic acid) and 3 g of polygonum cuspidatum extract powder (2.7 g of resveratrol), stirring for 5 minutes, adding 20 g of microcapsules containing scutellaria baicalensis extracts (2.7 g of baicalin), and continuously stirring until the microcapsules are completely dissolved;

3. preparing the liquid crystal nano lipid liquid with ultraviolet resistance and blue light resistance:

a. adding 0.9 g of lecithin, 0.45 g of caprylic/capric triglyceride, 0.45 g of behenyl alcohol, 0.45 g of stearyl alcohol, 0.3 g of PEG-20 phytosterol, 0.3 g of cetyl alcohol and 0.15 g of glyceryl stearate into 81 g of water, heating to 80 ℃ to dissolve to form a water phase;

b. at the temperature of 80 ℃, under the condition of homogenization, 7 g of isononyl isononanoate and 2 g of rosehip oil are put into an aqueous phase and homogenized for 5 minutes;

c. cooling the mixture prepared in the step b to 45 ℃, adding 6 g of the anti-ultraviolet and anti-blue light composition prepared in the step 2, and homogenizing for 1 minute;

d. and c, homogenizing the mixture prepared in the step c for 3 times at high pressure and 850bar to obtain the anti-ultraviolet and anti-blue-light liquid crystal nano-liposome liquid.

Example 2

1. Preparing microcapsules containing scutellaria baicalensis extracts: dispersing 15 g of scutellaria baicalensis extract powder (14 g of baicalin) into a proper amount of deionized water, adding 0.5 g of arginine to adjust the pH value of the solution to be 6.5, heating the solution to 60 ℃, adding 84.5 g of hydroxypropyl cyclodextrin, stirring until the hydroxypropyl cyclodextrin is completely dissolved, and cooling at 6 ℃ to obtain the microcapsule containing the scutellaria baicalensis extract. After filtration, the mixture was rotary evaporated at 60 ℃ to a dry powder.

a. adding 0.5 g lecithin, 0.3 g caprylic/capric triglyceride, 0.4 g stearyl alcohol, 0.3 g cetyl alcohol, 0.5 g glyceryl stearate to 86 g water, heating to 80 deg.C to dissolve to form water phase;

b. under the condition of keeping the temperature at 80 ℃, 6 g of caprylic/capric triglyceride and 2 g of seabuckthorn fruit oil are put into an aqueous phase and homogenized for 5 minutes;

c. cooling the mixture prepared in the step b to 45 ℃, adding 4 g of the anti-ultraviolet and anti-blue light composition prepared in the step 2, and homogenizing for 1 minute;

Example 3

a. adding 1.5 g of lecithin, 1 g of caprylic/capric triglyceride, 0.5 g of behenyl alcohol, 1 g of stearyl alcohol and 1 g of PEG-20 phytosterol into 76 g of water, and heating to 80 ℃ to dissolve to form a water phase;

b. under the condition of keeping the temperature at 80 ℃, 8 g of isooctyl palmitate and 2 g of tea tree oil are put into a water phase and homogenized for 5 minutes;

c. cooling the mixture prepared in the step b to 45 ℃, adding 9 g of the anti-ultraviolet and anti-blue light composition prepared in the step 2, and homogenizing for 1 minute;

Example 4

1. Preparing microcapsules containing scutellaria baicalensis extracts: dispersing 15 g of scutellaria baicalensis extract powder (14 g of baicalin) into a proper amount of deionized water, adding 0.5 g of arginine to adjust the pH value of the solution to be 6.5, heating the solution to 60 ℃, adding 84.5 g of hydroxypropyl cyclodextrin, stirring until the hydroxypropyl cyclodextrin is completely dissolved, and cooling at 6 ℃ to obtain the microcapsule containing the scutellaria baicalensis extract. Filtering, and rotary evaporating at 60 deg.C to obtain dry powder;

2. preparation of uv-and blue-light-resistant compositions: mixing 38 g of ethoxydiglycol and 26 g of water, heating to 45 ℃, keeping the temperature constant, stirring, slowly adding 12 g of sophora flower bud extract powder (11 g of troxerutin), 7 g of ligusticum chuanxiong hort extract powder (6 g of ferulic acid) and 2 g of polygonum cuspidatum extract powder (1.8 g of resveratrol), stirring for 10 minutes, adding 15 g of microcapsules containing scutellaria baicalensis extracts (2 g of baicalin), and continuously stirring until the microcapsules are completely dissolved.

a. adding 1.05 g of lecithin, 0.6 g of caprylic/capric triglyceride, 0.45 g of behenyl alcohol and 0.6 g of glyceryl stearate into 81 g of water, and heating to 80 ℃ to dissolve to form a water phase;

b. at a temperature of 80 ℃, under the condition of homogenization, 7 g of isononyl isononanoate and 2 g of rose hip oil are put into an aqueous phase and homogenized for 5 minutes;

And (3) performance detection:

UV detection spectra of UV and blue light resistant compositions prepared in examples 1 and 4

1. Instruments and materials

An ultraviolet/visible spectrophotometer; an analytical balance; quartz pool

2. Experimental procedure

(1) Taking a proper amount of ethanol, and preparing 0.01 per mill of ethylhexyl methoxycinnamate (MC80) solution serving as a control group;

(2) taking a proper amount of water, preparing 0.02 per mill, 0.04 per mill and 0.06 per mill of solutions of the anti-ultraviolet and anti-blue light composition prepared in the example 1 into an experimental group;

(2) switching on a power supply of an ultraviolet spectrophotometer instrument and preheating, and setting a detection wavelength: 280 nm-400 nm;

(3) placing the quartz cuvette filled with the solvent in a photometer, and adjusting the zero point of the instrument;

(4) and sequentially detecting ultraviolet absorption peak images of a control group and an experimental group with the prepared gradient concentration content.

3. Results of the experiment

The results of the uv detection spectra of the uv-resistant and blue-resistant compositions prepared in examples 1 and 4 are shown in figures 1 and 9, respectively: the compositions prepared in examples 1 and 4 have higher absorption at 280nm to 400nm, and especially after 340nm, the absorption value is obviously higher than that of a standard sample.

In vitro blue light protection efficacy of the UV and blue light resistant compositions prepared in example 1 were tested

1. Instruments and materials

An ultraviolet/visible spectrophotometer; an analytical balance; quartz pool

2. Experimental procedure

(1) Taking a proper amount of deionized water, and preparing 0.1%, 0.25%, 0.7%, 2% and 5% concentration of an aqueous solution of the ultraviolet-resistant and blue-light-resistant composition prepared in the example 1;

(2) switching on a power supply of an ultraviolet spectrophotometer instrument and preheating, and setting a detection wavelength: 400 nm-500 nm;

(3) placing the quartz cuvette filled with deionized water in a photometer, and adjusting the zero point of the photometer;

(4) the transmittance of the aqueous solution of the prepared graded concentration samples of the uv-and blue-resistant compositions of example 1 was sequentially measured.

3. The results of the detection are shown in FIG. 2.

As can be seen from FIG. 2, when the concentration of the UV and blue light resistant composition prepared in example 1 was 0.25%, the transmittance at a wavelength of 400nm was zero and the effect of protecting against blue light was initially achieved; when the concentration of the uv and blue light resistant composition prepared in example 1 was 5%, the light transmittance at a wavelength of 440nm was nearly zero, and the blue light was substantially completely absorbed, and the blue light was substantially completely protected in the wavelength range where damage to the skin occurred.

Detection of particle size detection and polarized light microscope detection of the UV-and blue-resistant liquid Crystal Nanohipids prepared in examples 1-4

The particle size detection experiment method comprises the following steps:

1. taking a proper amount of the liquid crystal nano lipid liquid sample prepared in the example 1-4, diluting the liquid crystal nano lipid liquid sample by 100 times with deionized water in a 50ml plastic centrifuge tube, uniformly mixing the liquid crystal nano lipid liquid sample with a vortex instrument, and standing the liquid crystal nano lipid liquid sample for 3 minutes;

2. and taking a proper amount of diluted to-be-detected sample which is uniformly mixed, placing the diluted to-be-detected sample in a particle size instrument detection dish, and detecting the particle size of the sample by using a Malvern particle size instrument.

The liquid crystal structure polarizing microscope observation method of the liquid crystal nano lipid liquid comprises the following steps:

1. placing the liquid crystal nano lipid liquid samples prepared in the examples 1-4 in a constant temperature box at 25 ℃ for 12 hours, taking a small amount of samples on a glass slide by using a glass rod, covering a cover glass and slightly pressing, wherein the strength should be paid attention during the pressing process so that the liquid crystal structure in the emulsion is convenient to observe and the liquid crystal structure in the emulsion is prevented from being damaged;

2. and observing and shooting liquid crystal structure images and quantity distribution of different samples under polarized light.

The detection results are as follows:

the results of the particle size detection graph and the polarized light microscope liquid crystal structure detection graph of the ultraviolet and blue light resistant liquid crystal nano lipid solution prepared in example 1 are shown in fig. 3 and 4, and the particle size of the liquid crystal nano lipid solution is substantially distributed between about 80-500 nm; the liquid crystal structure has a large number, uniform size and complete structure.

The results of the particle size detection graph and the polarized light microscope liquid crystal structure detection graph of the ultraviolet and blue light resistant liquid crystal nano lipid liquid prepared in example 2 are shown in fig. 5 and 6, and the particle sizes of the liquid crystal nano lipid liquid are substantially distributed between about 100-1000 nm; the liquid crystal structure has more number, more uniform size and complete structure.

The results of the particle size detection graph and the polarized light microscope liquid crystal structure detection graph of the ultraviolet and blue light resistant liquid crystal nano lipid liquid prepared in example 3 are shown in fig. 7 and 8, and the particle sizes of the liquid crystal nano lipid liquid are substantially distributed between about 100-900 nm; the liquid crystal structure has less number, uneven size, less liquid crystal and incomplete structure.

The results of the particle size detection graph and the polarized light microscope liquid crystal structure detection graph of the ultraviolet and blue light resistant liquid crystal nano lipid liquid prepared in example 4 are shown in fig. 10 and 11, and the particle sizes of the liquid crystal nano lipid liquid are substantially distributed between about 100-900 nm; the liquid crystal structure has small number, uneven size, a large amount of large liquid crystal and incomplete partial structure.

Claims

1. An anti-ultraviolet and anti-blue-light liquid crystal nano-liposome liquid, which is characterized by comprising the following components: based on the total weight of the liquid crystal nano-liposome liquid, 1-10 wt% of liquid crystal emulsifier, 5-15 wt% of grease, 2-20 wt% of ultraviolet-resistant and blue-resistant composition and 60-86 wt% of water are used, and the sum of all the components of the liquid crystal nano-liposome liquid is one hundred percent; wherein the anti-UV and anti-blue light composition comprises troxerutin, ferulic acid, resveratrol and baicalin; based on the total weight of the composition, the content of troxerutin is 9-40 wt%, the content of ferulic acid is 5-30 wt%, the content of resveratrol is 0.8-20 wt%, and the content of baicalin is 0.8-10 wt%; wherein, the sum of the components of the composition is one hundred percent; wherein the liquid crystal emulsifier is lecithin and a group consisting of one or more of caprylic/capric triglyceride, behenyl alcohol, stearyl alcohol, PEG-20 phytosterol, cetyl alcohol and glyceryl stearate; the preparation method of the liquid crystal nano-liposome liquid comprises the following steps:

(2) slowly adding the grease into the water phase under the homogenization condition, and homogenizing for a period of time;

(3) cooling the system in the step (2) to 40-50 ℃, adding the anti-ultraviolet and anti-blue light composition, and homogenizing for a period of time;

(4) and (4) homogenizing the system in the step (3) under high pressure.

2. The anti-ultraviolet and anti-blue-light liquid crystal nanoliposome liquid as claimed in claim 1, wherein the content of troxerutin is 14-30 wt%, the content of ferulic acid is 6-20 wt%, the content of resveratrol is 2-10 wt%, the content of baicalin is 2-8 wt%.

3. The UV and blue light resistant liquid crystal nanoliposome liquid according to claim 1, wherein the oil is synthetic oil and/or vegetable oil.

4. The UV and blue light resistant liquid crystal nanoliposome liquid according to claim 3, wherein the synthetic oil is one or more of isononyl isononanoate, caprylic/capric triglyceride and isooctyl palmitate; the vegetable oil is one or more of rose fruit oil, sea buckthorn fruit oil, tea tree oil and wheat germ oil.

5. The UV and blue light resistant liquid crystal nanoliposome liquid according to claim 1, wherein the composition further comprises one or more of quercetin, 4-hydroxy-3-butyl-concentrated lactone, emodin and baicalein.

6. The UV and blue light resistant liquid crystal nanoliposome according to any one of claims 1 to 5, wherein the troxerutin is derived from flos Sophorae Immaturus extract, the ferulic acid is derived from rhizoma Ligustici Chuanxiong extract, the resveratrol is derived from rhizoma Polygoni Cuspidati extract, and the baicalin is derived from radix Scutellariae extract.

7. The UV and blue light resistant liquid crystal nanoliposome liquid according to claim 6, wherein the Ligusticum chuanxiong Hort extract, the Polygonum cuspidatum extract or the Scutellaria baicalensis extract is in the form of microcapsule.

8. The UV and blue light resistant liquid crystal nano lipid liquid according to any one of claims 1 to 5, wherein the liquid crystal nano lipid liquid further comprises one or more of an antioxidant, a humectant and a preservative.

9. Use of the UV and blue light resistant liquid crystalline nano-liposome solution according to any one of claims 1 to 8 in the preparation of cosmetics.

10. The use of claim 9, wherein the uv and blue resistant liquid crystalline nano lipidic solution is present in the cosmetic in an amount of 2 to 30% by weight.