CN111119817A - Method for compositely displacing oil by internal and external source functional microorganisms - Google Patents
Method for compositely displacing oil by internal and external source functional microorganisms Download PDFInfo
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- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention belongs to the technical field of tertiary oil recovery, and particularly relates to a method for microbial compound oil displacement by internal and external source functions. The method comprises the following steps: screening a test oil reservoir; screening an endogenous functional microbial activator system; screening exogenous functional microorganisms; preparing an exogenous functional microorganism slow-release system; determining an injection process of an exogenous functional microorganism slow-release system; and (4) field test and evaluation of field test effect. The invention has simple implementation process and strong pertinence and reliability; meanwhile, the method has the advantages of low investment cost and good field test effect, and the input-output ratio is greater than 1: 5, increasing the recovery rate value by more than 20%. Therefore, the invention can be widely applied to the technical field of microbial oil recovery.
Description
Technical Field
The invention belongs to tertiary oil recovery, and particularly relates to a method for microbial compound oil displacement by internal and external source functions.
Background
The microbial oil displacement technology is an environment-friendly and sustainable oil extraction technology, and provides a new technical choice for further improving the recovery ratio of the oil field. The technology can be divided into endogenous microbial oil displacement technology and exogenous microbial oil displacement technology according to different microbial sources. Wherein, the endogenous microorganism oil extraction technology has low cost, strong adaptability and wider application prospect. The early-stage oil reservoir microbial community general investigation finds that most of oil reservoir environments contain abundant endogenous microbes, and the endogenous microbes can be directly utilized to drive oil. But part of the oil reservoirs lack microorganisms with oil displacement function or the microorganisms with oil displacement function in the oil reservoirs are difficult to be effectively and directionally activated, thereby limiting the application range and the effect of the endogenous microbial oil displacement technology.
Aiming at the oil reservoirs, the aim of improving the crude oil recovery ratio by microorganisms can be fulfilled by adding exogenous functional microorganisms to perfect a microorganism population structure. At present, exogenous functional microorganisms are often directly injected into an oil reservoir, and due to the detention and limited migration capacity of the microorganisms in the oil reservoir, most of the exogenous functional microorganisms are enriched and propagated in a stratum with low oil saturation near an injection well, the exogenous functional microorganisms cannot reach the deep part of the oil reservoir and cannot fully contact with residual oil with high oil saturation in the deep part of the oil reservoir, so that the crude oil action efficiency of the microorganisms is greatly reduced, and the oil displacement effect of the microorganisms is influenced.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a method for internal and external source function microbial compound oil displacement. The invention has simple implementation process, strong pertinence and reliability, and effectively enlarges the application range and application effect of the endogenous microbial oil displacement technology.
The invention discloses a method for internal and external source function microorganism composite oil displacement, which is characterized by comprising the following steps:
(1) screening of test reservoirs
The screening criteria for the test reservoirs were: oil deposit temperature is less than 95 ℃, stratum crude oil viscosity is less than 5000mPa.s, stratum water mineralization degree is less than 50000mg/L, and permeability is more than 100 multiplied by 10-3μm2And one or two endogenous functional microorganisms are present in the reservoir.
The endogenous functional microorganisms are biosurfactant-producing microorganisms, biopolymer-producing microorganisms and biogas-producing microorganisms.
The biosurfactant producing microorganism is one of geobacillus, bacillus, pseudomonas, acinetobacter and rhodococcus; the biopolymer-producing microorganism is one of sphingomonas, alcaligenes, xanthomonas and yeast; the microorganism producing the biogas is one of methanogen and hydrogen producing bacteria.
(2) Screening of endogenous functional microbial activator systems
The screening of the endogenous functional microbial activator system comprises the following specific steps: placing 100mL of formation water of a test oil reservoir into a culture bottle, optimizing the concentration of a long-acting carbon source, a nitrogen source and a phosphorus source of the microorganism by adopting an orthogonal experiment, carrying out static culture for 20-30 d at the temperature of the test oil reservoir, and determining a microorganism activator system with the endogenous function of the test oil reservoir according to the number of activated microorganisms.
(3) Screening of exogenous functional microorganisms
The screening of exogenous functional microorganisms comprises the following specific steps: placing 100mL of test oil reservoir formation water into a culture bottle, and adding 2-8% of exogenous functional microorganisms and nutrient solution thereof and 10-15% of crude oil; then carrying out static culture for 10-15 d at the temperature of the test oil reservoir; screening and evaluating the experimental result, and screening the exogenous functional microorganism according to the experimental result.
The exogenous functional microorganisms include biosurfactant-producing microorganisms, biopolymer-producing microorganisms and biogas-producing microorganisms.
The screening and evaluation indexes of the biosurfactant-producing microorganism are that the emulsification index is more than 90 percent, and the dominance ratio of the biosurfactant-producing microorganism in the whole microorganism population is more than 30 percent; the screening and evaluation indexes of the biopolymer-producing microorganism are that the solution viscosity is more than 20mPa & s, and the biopolymer-producing microorganism accounts for more than 30% of the whole microorganism population; the screening and evaluation indexes of the microorganisms generating the biogas are that the air pressure is more than 0.5MPa, and the ratio of the microorganisms generating the biogas in the whole microbial population is more than 30%.
(4) Preparation of exogenous functional microbial slow-release system
The preparation method of the exogenous functional microorganism slow-release system comprises the following steps:
① selecting a beaker with the volume of 1L, and adding the formation water of the test oil reservoir into the beaker;
② adding the carrier 1 into the beaker, and stirring uniformly at the rotation speed of 300-500 rpm;
③ adding the carrier 2 and the fermentation liquid of the exogenous functional microorganism into the beaker in sequence, stirring uniformly at 800-1000 rpm, and then pasting for 4-8 h at 60-80 ℃;
④ cooling to 35-45 deg.C, adding cross-linking agent for cross-linking, extracting with chloroform, drying and grinding to obtain the sustained-release system.
Wherein the carrier 1 is one of xanthan gum, welan gum, gellan gum and pullulan, and the addition amount is 0.5-2 g/L.
The carrier 2 is one of cassava flour, amylopectin and wheat middling, and the adding amount is 2-8 g/L.
The cross-linking agent is one of borax and methylene bisacrylamide, and the addition amount is 0.1-1 g/L.
The addition amount of the fermentation liquor of the exogenous functional microorganisms is 50-100 g/L.
(5) In-situ implant process determination
Injecting an exogenous functional microorganism slow-release system into a water injection well of a test oil reservoir by using a high-pressure pump truck, wherein the injection amount is 0.2-0.3 PV, and the injection speed is 10-20 m3H; secondly, injecting an endogenous functional microbial activator system, wherein the injection amount is 0.1-0.2 PV, and the injection speed is 5-15 m3/h。
(6) On-site test and evaluation of on-site test Effect
And (5) carrying out a field test according to the process determined in the step (5), and evaluating the field test effect after the field test is finished, wherein the evaluation indexes comprise the dominance ratio of the functional microorganisms, the yield improvement value and the input-output ratio.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the invention has the characteristic of wide oil reservoir application range, and is suitable for oil reservoir temperature of less than 95 ℃, crude oil viscosity of less than 5000mPa.s, stratum water mineralization degree of less than 50000mg/L, and permeability of more than 100 multiplied by 10-3μm2The reservoir of (a);
(2) the method has the advantages of simple implementation process, strong pertinence and reliability, and effectively expands the application range and the application effect of the endogenous microbial oil displacement technology;
(3) the invention effectively enhances the swept volume of the functional microorganism, thereby effectively increasing the contact area with crude oil, further improving the crude oil action efficiency of the functional microorganism, and the dominant ratio of internal and external functional bacteria in the microbial population structure is more than 60%; meanwhile, the method has the advantages of low investment cost and good field test effect, and the input-output ratio is greater than 1: 5, increasing the recovery rate value by more than 20%.
According to the invention, firstly, exogenous functional microorganisms lacking in the oil reservoir are injected into the water injection well of the oil reservoir, and the exogenous functional microorganisms are prepared into a slow release system, so that the exogenous microorganisms cannot be greatly enriched in a zone with low oil saturation near the well, and can be slowly released along with the process that the injected water enters the deep part of the oil reservoir, thereby being fully and effectively contacted with the residual oil in a high saturation zone; and an activator system suitable for the growth of the endogenous microorganisms is injected, and because the activator system contains high-molecular long carbon chain substances, the consumption and utilization of the microorganisms are slow, the microorganisms cannot be metabolized and exhausted by the endogenous microorganisms in a near-wellbore region, and the endogenous microorganisms in the deep oil reservoir can be effectively activated, so that the effective utilization rate of the activator system and the activation efficiency of the endogenous microorganisms in the deep oil reservoir are improved. By the process, exogenous functional microorganisms lacking in an oil reservoir can be effectively supplemented, the oil displacement microorganism population structure is improved, the number and metabolic activity of functional bacteria in the oil reservoir are improved, endogenous microorganisms in the deep part of the oil reservoir can be fully activated, the crude oil action effect of the endogenous microorganisms is exerted, the microbial oil displacement is carried out by combining the synergistic effect of the endogenous and exogenous microorganisms, and the field test effect of the microbial oil displacement is integrally improved.
Detailed Description
Example 1: take a block A of the Shengli oil field as an example
The oil reservoir has the buried depth of 1362-1650 m, the oil reservoir temperature of 75 ℃, the oil layer pressure of 10.07MPa and the permeability of 2870 multiplied by 10-3μm2The salinity of formation water is 3327mg/L, the viscosity of crude oil is 1586mPa.s, and the pore volume is 5.2 multiplied by 105m3Geological reserve of 1.2X 105t, the steps for implementing the invention are as follows:
(1) screening of test reservoirs
Test block a: oil reservoir temperature of 75 ℃, crude oil viscosity of 1586mPa.s, stratum water mineralization degree of 3327mg/L, and permeability of 2870 multiplied by 10-3μm2The existence of methane-producing microorganism methane-producing bacteria of 1.0 x 10 in the formation water22.0X 10 of the microorganism sphingomonas campestris/mL and producing biopolymer2one/mL, lack of biosurfactant-producing microorganisms, meeting the reservoir screening criteria of the present invention.
(2) Screening of endogenous functional microbial activator systems
100mL of formation water of the test block A was taken and placed in a culture flask, and the concentration of pectin, corn steep liquor dry powder and dipotassium hydrogen phosphate was optimized by an orthogonal experiment as shown in Table 1.
TABLE 1 endogenous microbial multiple activator System optimization factor-horizon
L9 (3) is selected4) Orthogonal table, see table 2.
TABLE 2 optimization of the endogenous microbial activator System orthogonal Experimental tables
The above combinations were subjected to static culture at 75 ℃ for 25 days, and the number of microorganisms activated with the activator was evaluated, and Table 3 shows the results of experiments using the number of microorganisms as an index.
TABLE 3 orthogonal experimental design and experimental results using the number of activated microorganisms as an index
According to the results of the orthogonal experiments and the analysis of mean and range of the results in Table 3, the endogenous microorganism activator system in Block A consisted of 0.5% by mass of pectin, 0.3% by mass of dry corn steep liquor and 0.03% by mass of dipotassium hydrogen phosphate, and the activated microorganism concentration was 6.0X 108one/mL.
(3) Screening of exogenous functional microorganisms
Placing 100mL of stratum water of the test block A into a culture bottle, and adding 5% of surfactant-producing microorganisms and nutrient solution thereof and 10% of crude oil; then, the mixture is subjected to static culture at 75 ℃ for 10 days, and the emulsion index and the microbial population structure ratio are analyzed.
TABLE 4 screening and evaluation indexes of biosurfactant-producing microorganisms
| Biosurfactant-producing microorganisms | Emulsification index (%) | Percentage of population structure (%) |
| Geobacillus | 92 | 42 |
| Bacillus | 96 | 45 |
| Pseudomonas sp | 90 | 40 |
| Acinetobacter | 87 | 36 |
| Rhodococcus sp | 82 | 33 |
As can be seen from table 4: the bacillus emulsification index and the population structure ratio are the highest, and are respectively 96% and 45%, so that the screened exogenous functional microorganism producing the biosurfactant is bacillus.
(4) Preparation of exogenous functional microbial slow-release system
① selecting a beaker with the volume of 1L, and adding the formation water of the test block A into the beaker;
② adding 1g/L xanthan gum into the beaker, and stirring uniformly at the rotation speed of 300 rpm;
③ adding 2g/L of cassava powder and 50g/L of bacillus fermentation liquor into a beaker in sequence, stirring uniformly at 800rpm, and then standing at 60 ℃ for gelatinization for 8 h;
④ cooling to 45 deg.C, adding 1g/L borax for crosslinking, extracting with chloroform, drying, and grinding to obtain Bacillus slow release system.
(5) In-situ implant process determination
Injecting a bacillus slow-release system into a water injection well of the test block A by using a high-pressure pump truck, wherein the injection amount is 1.04 multiplied by 105m3The injection speed is 10m3H; secondly, injecting an endogenous functional microbial activator system: the mass concentration of pectin is 0.5%, the mass concentration of corn steep liquor dry powder is 0.3%, the mass concentration of dipotassium hydrogen phosphate is 0.03%, and the injection amount is 0.52 multiplied by 105m3The injection speed is 5m3/h。
(6) On-site test and evaluation of on-site test Effect
Evaluation of test results: in the test block A, the microbial oil displacement field test is carried out by utilizing the compounding of the internal and external functional microorganisms, the proportion of the functional microorganisms in the microbial population structure is 78 percent, and the cumulative oil increase is 2.544 multiplied by 104t, the recovery ratio of the crude oil is improved by 21.2 percent, and the input-output ratio is 1: 5.8, obtaining good economic benefit.
Example 2: take a block G of the Shengli oil field as an example
The oil reservoir has the buried depth of 1054-1220 m, the oil reservoir temperature of 63 ℃, the oil layer pressure of 9.25MPa and the permeability of 950 multiplied by 10-3μm2The salinity of the formation water is 18526mg/L, the viscosity of the crude oil is 1870mPa.s, and the geological reserve of a test block is 2.5 multiplied by 105t, pore volume 7.2X 105m3The method comprises the following steps:
(1) reservoir screening
Test block G: oil reservoir temperature of 63 ℃, crude oil viscosity of 1870mPa.s, formation water mineralization of 18526mg/L, permeability of 950 multiplied by 10-3μm2The presence of the biopolymer producing microorganism Xanthomonas (2.0X 10) in the formation water2Microbial bacillus 3.0 x 10 strain/ml and biosurfactant producer2Per ml, lack of biogas-producing microorganisms, and meet the reservoir screening criteria of the present invention.
(2) Screening of endogenous functional microbial activator systems
100mL of stratum water of a test block G is placed in a culture bottle, an endogenous microorganism activator system consisting of the sphingosine gum, the peptone and the diammonium hydrogen phosphate is subjected to orthogonal experiment, and an orthogonal experiment table with three factors and three levels is designed, and is shown in table 5.
TABLE 5 endogenous microbial activator System optimization factor-horizon
L9 (3) is selected4) Orthogonal table, see table 6.
TABLE 6 optimization of the endogenous microbial activator System orthogonal Experimental tables
The above combinations were subjected to static culture at 63 ℃ for 30 days, and the concentrations of the microorganisms activated with the activators were evaluated, and Table 7 shows the results of experiments using the concentrations of the microorganisms as indices.
TABLE 7 orthogonal experimental design and experimental results using the number of activated microorganisms as an index
| In the column | 1 | 2 | 3 | Microorganism concentration (10)8one/mL) |
| Factors of the fact | Sphingol gum | Peptone | Diammonium hydrogen phosphate | Results of the experiment |
| Experiment 1 | 1 | 1 | 1 | 2.9 |
| Experiment 2 | 1 | 2 | 2 | 4.6 |
| Experiment 3 | 1 | 3 | 3 | 1.5 |
| Experiment 4 | 2 | 1 | 2 | 5.2 |
| Experiment 5 | 2 | 2 | 3 | 7.6 |
| Experiment 6 | 2 | 3 | 1 | 4.0 |
| Experiment 7 | 3 | 1 | 3 | 3.6 |
| Experiment 8 | 3 | 2 | 1 | 2.8 |
| Experiment 9 | 3 | 3 | 2 | 6.6 |
| Mean value 1 | 3.000 | 3.900 | 3.233 | |
| Mean value 2 | 5.600 | 5.000 | 5.467 | |
| Mean value 3 | 4.333 | 4.033 | 4.233 | |
| Extreme difference | 2.600 | 1.100 | 2.234 |
According to orthogonal experimental results and analysis of mean and range, the endogenous microorganism activator system of the test block G consists of 0.4 percent of sphingan gum, 0.15 percent of peptone and 0.01 percent of diammonium hydrogen phosphate, and the activated microorganism concentration is 7.6 multiplied by 108one/mL.
(3) Screening of exogenous functional microorganisms
Placing 100mL of stratum water of a test block G in a culture bottle, and adding 2% of biogas-producing microorganisms and nutrient solution thereof and 15% of crude oil; and then carrying out static culture for 15d at the temperature of the test oil reservoir, and carrying out air pressure and microbial population structure ratio analysis.
TABLE 8 screening and evaluation indexes of biogas-producing microorganisms
| Producing biogas microorganisms | Air pressure (MPa) | Percentage of population structure (%) |
| Methanogenic bacteria | 0.8 | 42 |
| Hydrogen producing bacteria | 0.7 | 35 |
As can be seen from table 8: the gas pressure and the population structure of methanogens are respectively 0.8MPa and 42%, and the gas pressure and the population structure of hydrogen-producing bacteria are respectively 0.7MPa and 35%, so that the screened exogenous functional microorganism producing biogas is methanogens.
(4) Preparation of exogenous functional microbial slow-release system
① selecting a beaker with the volume of 1L, and adding the formation water of the test block A into the beaker;
② adding 2g/L welan gum into the beaker, and stirring uniformly at the rotation speed of 500 rpm;
③ sequentially adding 8g/L wheat middling and 80g/L methanogen fermentation liquor into a beaker, uniformly stirring at 1000rpm, and then standing at 70 ℃ for gelatinization for 4 h;
④ cooling to 40 deg.C, adding 0.1g/L borax for crosslinking, extracting with chloroform, drying, and grinding to obtain methanogen slow release system.
(5) In-situ implant process determination
Injecting a methanogen slow release system into a water injection well of the test block G by using a high-pressure pump truck, wherein the injection amount is 0.75 multiplied by 105m3The injection speed is 20m3H; secondly, injecting an endogenous functional microbial activator system: the mass concentration of the sphingol gum is 0.4 percent, and the proteinPeptone concentration of 0.15 wt%, diammonium hydrogen phosphate concentration of 0.01 wt%, and injection amount of 0.5 × 105m3The injection speed is 15m3/h。
(6) On-site test and evaluation of on-site test Effect
Evaluation of test results: in the test block G, the microbial oil displacement field test is carried out by utilizing the compounding of the internal and external functional microbes, the proportion of the functional microbes in the microbial population structure is 75 percent, and the accumulated oil increase is 0.54 multiplied by 105t, the recovery ratio of the crude oil is improved by 21.5 percent, and the input-output ratio is 1: 6.2, obtaining good economic benefit.
Example 3: take a block H of the Shengli oil field as an example
The oil reservoir has the buried depth of 940-1120 m, the oil reservoir temperature of 57 ℃, the oil layer pressure of 8.25MPa and the permeability of 1250 multiplied by 10-3μm2The salinity of formation water is 3546mg/L, the viscosity of crude oil is 960mPa.s, and the geological reserve of a test block is 1.1 multiplied by 105t, pore volume 5.0X 105m3The method comprises the following steps:
(1) reservoir screening
And (3) reservoir block H: oil reservoir temperature 57 ℃, crude oil viscosity 960mPa.s, formation water mineralization degree 3546mg/L, penetration rate 1250 multiplied by 10-3μm2In the formation water, Bacillus subtilis (5.0X 10) as biosurfactant-producing microorganism is present22.0X 10 methanogen bacteria per mL and gas-producing microorganisms2one/mL, lack of biopolymer-producing microorganisms, meet the reservoir screening criteria of the present invention.
(2) Screening of endogenous functional microbial activator systems
100mL of stratum water of a test block H is taken and placed in a culture bottle, an endogenous microorganism activator system consisting of xylitol, yeast powder and dipotassium hydrogen phosphate is subjected to orthogonal experiment, and an orthogonal experiment table with three factors and three levels is designed, and is shown in table 9.
TABLE 9 endogenous microorganism multiple activator System optimization factor-level Table
L9 (3) is selected4) Orthogonal table, see table 10.
TABLE 10 optimization of the orthogonal Experimental tables for the endogenous microbial activator System
The above combinations were subjected to static culture at 57 ℃ for 20 days, and the concentrations of the microorganisms activated with the activator were evaluated, and Table 11 shows the results of experiments using the number of microorganisms as an index.
TABLE 11 orthogonal experimental design and experimental results using number of activated microorganisms as an index
| In the column | 1 | 2 | 3 | Microorganism concentration (10)8one/mL) |
| Factors of the fact | Xylitol, its preparation method and use | Yeast powder | Dipotassium hydrogen phosphate | Results of the experiment |
| Experiment 1 | 1 | 1 | 1 | 2.9 |
| Experiment 2 | 1 | 2 | 2 | 3.2 |
| Experiment 3 | 1 | 3 | 3 | 1.9 |
| Experiment 4 | 2 | 1 | 2 | 4.0 |
| Experiment 5 | 2 | 2 | 3 | 4.8 |
| Experiment 6 | 2 | 3 | 1 | 3.5 |
| Experiment 7 | 3 | 1 | 3 | 3.8 |
| Experiment 8 | 3 | 2 | 1 | 3.0 |
| Experiment 9 | 3 | 3 | 2 | 2.6 |
| Mean value 1 | 2.667 | 3.567 | 3.133 | |
| Mean value 2 | 4.100 | 3.667 | 3.267 | |
| Mean value 3 | 3.133 | 2.667 | 3.500 | |
| Extreme difference | 1.433 | 1.000 | 0.367 |
According to the results of orthogonal experiments and the analysis of mean and range of differences in table 11, the H-block endogenous microorganism activator system consisted of 0.8% by mass xylitol, 0.1% by mass yeast powder, 0.01% by mass dipotassium hydrogen phosphate, and 4.8 × 10% by mass activated microorganism8one/mL.
(3) Screening of exogenous functional microorganisms
Placing 100mL of stratum water of a test block H into a culture bottle, and adding 8% of biopolymer-producing microorganisms and nutrient solution thereof and 12% of crude oil; then, standing and culturing the mixture for 12d at the temperature of 57 ℃, and respectively carrying out the index analysis of the aerogenic microorganism and the biopolymer-producing microorganism.
TABLE 12 screening and evaluation indexes of biopolymer-producing microorganisms
| Biopolymer producing microorganisms | Viscosity (mPa.s) | Percentage of population structure (%) |
| Sphingomonas reinhardtii | 72 | 35 |
| Alcaligenes sp | 70 | 32 |
| Xanthomonas campestris | 105 | 45 |
| Yeast | 63 | 37 |
As can be seen from table 12: the viscosity and the population structure of the Xanthomonas are the highest, respectively 105mPa.s and 45%, so that the screened exogenous functional microorganism producing the biopolymer microorganism is Xanthomonas.
(4) Preparation of exogenous functional microbial slow-release system
① selecting a beaker with the volume of 1L, and adding the formation water of the test block A into the beaker;
② adding 0.5g/L pullulan into the beaker, and stirring uniformly at the rotation speed of 400 rpm;
③ adding 4g/L amylopectin and 70g/L xanthomonas fermentation liquid into the beaker in sequence, stirring uniformly at 900rpm, and then standing at 80 ℃ for gelatinization for 5 h;
④ cooling to 35 deg.C, adding 0.5g/L methylene bisacrylamide for crosslinking, extracting with chloroform, drying, and grinding to obtain xanthomonas sustained-release system.
(5) In-situ implant process determination
Injecting a xanthomonas sustained-release system into a water injection well of the test block H by using a high-pressure pump truck, wherein the injection amount is 1.25 multiplied by 105m3The injection speed is 15m3H; secondly, injecting an endogenous functional microbial activator system: xylitol 0.8 wt%, yeast powder 0.1 wt%, dipotassium hydrogen phosphate 0.01 wt%, and injection amount 0.75 × 105m3The injection speed is 10m3/h。
(6) On-site test and evaluation of on-site test Effect
The statistics of test results shows that the microbial oil displacement field test is carried out in the test block H by utilizing the compounding of the internal and external source functional microorganisms, the proportion of the functional microorganisms in the microbial population structure is 80 percent, and the cumulative oil increment is 2.45 multiplied by 104t, enhanced crude oil recoveryThe yield reaches 22.3 percent, and the input-output ratio reaches 1: 6.8, obtaining good economic benefit.
Claims (15)
1. The method for internal and external source functional microorganism composite oil displacement is characterized by comprising the following steps:
(1) screening a test oil reservoir;
(2) screening an endogenous functional microbial activator system;
(3) screening exogenous functional microorganisms;
(4) preparing an exogenous functional microorganism slow-release system;
(5) determining an in-situ implantation process;
(6) and (4) field test and evaluation of field test effect.
2. The method for the complex oil displacement by the internal and external source functional microorganisms according to claim 1, characterized in that the screening standard of the test oil reservoir is as follows: oil deposit temperature is less than 95 ℃, stratum crude oil viscosity is less than 5000mPa.s, stratum water mineralization degree is less than 50000mg/L, and permeability is more than 100 multiplied by 10-3μm2And one or two endogenous functional microorganisms are present in the reservoir.
3. The method for the complex flooding by internal and external source functional microorganisms of claim 2, wherein the internal source functional microorganisms comprise biosurfactant-producing microorganisms, biopolymer-producing microorganisms and biogas-producing microorganisms.
4. The method for complex flooding by using internal and external source functional microorganisms as claimed in claim 3, wherein the biosurfactant-producing microorganism is one of Geobacillus, Bacillus, Pseudomonas, Acinetobacter and Rhodococcus.
5. The method for complex oil displacement by internal and external functional microorganisms according to claim 3, wherein the biopolymer-producing microorganism is one of sphingomonas, alcaligenes, xanthomonas and yeast.
6. The method for internal and external source function microorganism complex oil displacement according to claim 3, wherein the gas-producing microorganism is one of methanogen and hydrogenogen.
7. The method for the complex oil displacement by the internal and external source functional microorganisms according to claim 1, wherein the specific method for screening the internal source functional microorganism activator system is as follows: placing 100mL of formation water of a test oil reservoir into a culture bottle, optimizing the concentration of a long-acting carbon source, a nitrogen source and a phosphorus source of the microorganism by adopting an orthogonal experiment, carrying out static culture for 20-30 d at the temperature of the test oil reservoir, and determining a microorganism activator system with the endogenous function of the test oil reservoir according to the number of activated microorganisms.
8. The method for the composite oil displacement by the internal and external functional microorganisms according to claim 1, which is characterized in that the specific method for screening the external functional microorganisms is as follows: placing 100mL of test oil reservoir formation water into a culture bottle, and adding 2-8% of exogenous functional microorganisms and nutrient solution thereof and 10-15% of crude oil; then carrying out static culture for 10-15 d at the temperature of the test oil reservoir; screening and evaluating the experimental result, and screening the exogenous functional microorganisms according to the experimental result;
the exogenous functional microorganisms include biosurfactant-producing microorganisms, biopolymer-producing microorganisms and biogas-producing microorganisms.
9. The method for internal and external source microbial complex flooding according to claim 8, wherein the screening and evaluation indexes of biosurfactant-producing microbes are that the emulsification index is > 90%, and the dominance ratio of the biosurfactant-producing microbes in the whole microbial population is > 30%; the biological screening and evaluation indexes of the biopolymer are that the solution viscosity is more than 20mPa & s, and the ratio of biopolymer-producing microorganisms in the whole microbial population is more than 30%; the screening and evaluation indexes of the microorganisms generating the biogas are that the air pressure is more than 0.5MPa, and the ratio of the microorganisms generating the biogas in the whole microbial population is more than 30%.
10. The method for the composite oil displacement by the internal and external functional microorganisms according to claim 1, which is characterized in that the preparation of the external functional microorganism slow-release system comprises the following steps:
① selecting a beaker with the volume of 1L, and adding the formation water of the test oil reservoir into the beaker;
② adding the carrier 1 into the beaker, and stirring uniformly at the rotation speed of 300-500 rpm;
③ adding the carrier 2 and the fermentation liquid of the exogenous functional microorganism into the beaker in sequence, stirring uniformly at 800-1000 rpm, and then pasting for 4-8 h at 60-80 ℃;
④ cooling to 35-45 deg.C, adding cross-linking agent for cross-linking, extracting with chloroform, drying and grinding to obtain the sustained-release system.
11. The method for complex oil displacement by internal and external source functional microorganisms of claim 10, wherein the carrier 1 is one of xanthan gum, welan gum, gellan gum and pullulan, and the addition amount is 0.5-2 g/L.
12. The method for internal and external source function microbial compound oil displacement according to claim 10, wherein the carrier 2 is one of tapioca flour, amylopectin and wheat middling, and the addition amount is 2-8 g/L.
13. The method for internal and external source function microbial compound oil displacement according to claim 10, wherein the cross-linking agent is one of borax and methylene bisacrylamide, and the addition amount is 0.1-1 g/L; the adding amount of the exogenous functional microorganism fermentation liquor is 50-100 g/L.
14. The method for the microbial compound flooding with internal and external source functions according to claim 1, wherein the field injection process specifically comprises the following steps: height of utilizationInjecting an exogenous functional microorganism slow-release system into a water injection well of a test oil reservoir by a pressure pump truck, wherein the injection amount is 0.2-0.3 PV, and the injection speed is 10-20 m3H; secondly, injecting an endogenous functional microbial activator system, wherein the injection amount is 0.1-0.2 PV, and the injection speed is 5-15 m3/h。
15. The method for the composite oil displacement by the internal and external source functional microorganisms, according to claim 1, is characterized in that the field test and the evaluation of the field test effect comprise the following specific steps: and (5) carrying out a field test according to the process determined in the step (5), and evaluating the field test effect after the field test is finished, wherein the evaluation indexes comprise the dominance ratio of the oil displacement functional microorganisms, the improvement of the recovery rate value and the input-output ratio.
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