CN111094324A - 一种溶瘤病毒、合成dna序列及其应用 - Google Patents

一种溶瘤病毒、合成dna序列及其应用 Download PDF

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CN111094324A
CN111094324A CN201980003419.3A CN201980003419A CN111094324A CN 111094324 A CN111094324 A CN 111094324A CN 201980003419 A CN201980003419 A CN 201980003419A CN 111094324 A CN111094324 A CN 111094324A
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oncolytic virus
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CN111094324B (zh
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蔡立刚
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Wuhan Boweide Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/145—Rhabdoviridae, e.g. rabies virus, Duvenhage virus, Mokola virus or vesicular stomatitis virus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
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  • Gastroenterology & Hepatology (AREA)
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  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

本发明公开了一种溶瘤病毒、合成DNA序列及其应用。所述溶瘤病毒其基因组上插入有外源碱性肽段的表达序列，并在生理过程中表达所述碱性肽段，使得其感染的宿主环境pH值增大。所述合成DNA序列用于表达碱性肽段，所述碱性肽段中碱性氨基酸含量超过60％。本发明提供的溶瘤病毒及合成DNA序列，应用于制备抗肿瘤药物，具有良好的抗肿瘤效果。

Description

PCT国内申请，说明书已公开。

Claims (23)

  1. PCT国内申请，权利要求书已公开。
CN201980003419.3A 2018-03-14 2019-03-14 一种溶瘤病毒、合成dna序列及其应用 Active CN111094324B (zh)

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US201862643166P 2018-03-14 2018-03-14
US62/643166 2018-03-14
PCT/CN2019/078117 WO2019174610A1 (zh) 2018-03-14 2019-03-14 一种溶瘤病毒、合成dna序列及其应用

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112538466A (zh) * 2020-12-10 2021-03-23 武汉博威德生物技术有限公司 一种柯萨奇病毒及其在制备抗肝癌药物中的应用

Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005074968A2 (en) * 2004-02-10 2005-08-18 Universiteit Maastricht Medical use of basic peptides
KR20090024387A (ko) * 2007-09-04 2009-03-09 가톨릭대학교 산학협력단 약독화된 콕사키바이러스게놈을 이용한 외래 유전자 발현시스템
US20100178684A1 (en) * 2006-12-21 2010-07-15 Woo Savio L C Transgenic oncolytic viruses and uses thereof
CN102099045A (zh) * 2008-01-29 2011-06-15 拜耳先灵医药股份有限公司 编码禽类细胞因子的减毒溶瘤性副粘病毒
WO2011100688A1 (en) * 2010-02-12 2011-08-18 Dana-Farber Cancer Institute, Inc. Improved antagonists of muc1
CN102548584A (zh) * 2009-05-06 2012-07-04 贝尔韦什生物医学研究学会 用于治疗癌症的溶瘤腺病毒
CN103221423A (zh) * 2010-09-24 2013-07-24 昂克斯治疗有限公司 溶瘤腺病毒载体及与其相关的方法和用途
WO2013110120A1 (en) * 2012-01-24 2013-08-01 Inter-K Pty Limited Peptide agents for cancer therapy
CN103981152A (zh) * 2014-04-16 2014-08-13 武汉博威德生物技术有限公司 一种柯萨奇病毒及其制备抗肿瘤药物之应用
CN104151402A (zh) * 2014-08-06 2014-11-19 苏州大学 病毒性心肌炎环肽疫苗及其制备方法
US20160008480A1 (en) * 2014-07-08 2016-01-14 Samsung Electronics Co., Ltd. Fusion protein comprising targeting moiety, cleavage site, and cell membrane penetrating domain, and use thereof
CN106632613A (zh) * 2017-01-17 2017-05-10 中国药科大学 与柯萨奇病毒腺病毒受体相关的亲和肽
CN107164338A (zh) * 2017-06-27 2017-09-15 镇江市卫克生物科技有限公司 一种重组溶瘤病毒及其应用
WO2018027316A1 (en) * 2016-08-09 2018-02-15 Alkayyal Almohanad Oncolytic rhabdovirus expressing il12

Patent Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005074968A2 (en) * 2004-02-10 2005-08-18 Universiteit Maastricht Medical use of basic peptides
US20100178684A1 (en) * 2006-12-21 2010-07-15 Woo Savio L C Transgenic oncolytic viruses and uses thereof
KR20090024387A (ko) * 2007-09-04 2009-03-09 가톨릭대학교 산학협력단 약독화된 콕사키바이러스게놈을 이용한 외래 유전자 발현시스템
CN102099045A (zh) * 2008-01-29 2011-06-15 拜耳先灵医药股份有限公司 编码禽类细胞因子的减毒溶瘤性副粘病毒
CN102548584A (zh) * 2009-05-06 2012-07-04 贝尔韦什生物医学研究学会 用于治疗癌症的溶瘤腺病毒
WO2011100688A1 (en) * 2010-02-12 2011-08-18 Dana-Farber Cancer Institute, Inc. Improved antagonists of muc1
CN103221423A (zh) * 2010-09-24 2013-07-24 昂克斯治疗有限公司 溶瘤腺病毒载体及与其相关的方法和用途
WO2013110120A1 (en) * 2012-01-24 2013-08-01 Inter-K Pty Limited Peptide agents for cancer therapy
CN103981152A (zh) * 2014-04-16 2014-08-13 武汉博威德生物技术有限公司 一种柯萨奇病毒及其制备抗肿瘤药物之应用
US20160008480A1 (en) * 2014-07-08 2016-01-14 Samsung Electronics Co., Ltd. Fusion protein comprising targeting moiety, cleavage site, and cell membrane penetrating domain, and use thereof
CN104151402A (zh) * 2014-08-06 2014-11-19 苏州大学 病毒性心肌炎环肽疫苗及其制备方法
WO2018027316A1 (en) * 2016-08-09 2018-02-15 Alkayyal Almohanad Oncolytic rhabdovirus expressing il12
CN106632613A (zh) * 2017-01-17 2017-05-10 中国药科大学 与柯萨奇病毒腺病毒受体相关的亲和肽
CN107164338A (zh) * 2017-06-27 2017-09-15 镇江市卫克生物科技有限公司 一种重组溶瘤病毒及其应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DAWN E. POST等: "Cancer Therapy with a Replicating Oncolytic Adenovirus Targeting the Hypoxic Microenvironment of Tumors", vol. 10, pages 8603 - 8612, XP055637037 *
喻启桂;: "肿瘤治疗的新突破:溶瘤病毒治疗", 安徽医药, no. 01, pages 9 - 15 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112538466A (zh) * 2020-12-10 2021-03-23 武汉博威德生物技术有限公司 一种柯萨奇病毒及其在制备抗肝癌药物中的应用

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