CN111073797A

CN111073797A - Circulating tumor cell step-by-step separation device

Info

Publication number: CN111073797A
Application number: CN202010143319.5A
Authority: CN
Inventors: 李胜; 郝继辉; 王振丹; 李�浩; 夏梅; 马莹
Original assignee: Shandong First Medical University and Shandong Academy of Medical Sciences
Current assignee: Shandong First Medical University and Shandong Academy of Medical Sciences
Priority date: 2020-03-04
Filing date: 2020-03-04
Publication date: 2020-04-28

Abstract

The invention provides a device for separating circulating tumor cells step by step, which separates and collects the circulating tumor cells step by step according to the size, and comprises a conical shell and an adjusting cone, wherein the adjusting cone is positioned in the shell and can move along the axial direction, an inlet is formed between the upper end of the adjusting cone and the upper end of the shell, and an outlet is formed between the lower end of the adjusting cone and the lower end of the shell.

Description

Circulating tumor cell step-by-step separation device

Technical Field

The invention belongs to the field of circulating tumor cell detection, and particularly relates to a circulating tumor cell step-by-step separation device.

Background

Circulating tumor cells generally refer to cells that are derived from primary tumor tissue, are exfoliated and stained into peripheral blood of a human body, can colonize different tissues during the process of circulating cell metastasis, and can be found on a plurality of solid-phase tumors after metastasis, so that the circulating tumor cells can be used as an important marker for early tumor diagnosis. In addition, circulating tumor cells play an important role in the metastasis and recurrence of tumors. Therefore, the method has very important significance for the research of the physiological mechanism of the circulating tumor cells. However, the number of circulating tumor cells and their absolute concentration in human peripheral blood is very low, making the study of circulating tumor cells very difficult. Sorting and capturing of circulating tumor cells has therefore become an important issue of research.

The detection of CTCs is to separate tumor cells from blood cells in blood, wherein one separation method is to separate and enrich CTCs and blood cells according to their physical characteristics. For example, in the microfluidic cell capture chip in the prior art, a human body fluid sample passes through the microfluidic cell capture chip, so as to collect circulating tumor cells with a specific size, and then perform the next experiment. The size of circulating tumor cells is also different, and if the circulating tumor cells can be collected separately for research and attack, the circulating tumor cells play an important role in inhibiting the clinical application of CTCs. However, the existing microfluidic cell capture chip does not have the function of gradually separating tumor cells.

Disclosure of Invention

The invention aims to provide a device for separating circulating tumor cells step by step, which separates and collects the circulating tumor cells step by step according to the size.

The invention is realized by the following technical scheme:

the device for separating the circulating tumor cells step by step is characterized by comprising a conical shell and an adjusting cone, wherein the adjusting cone is positioned in the shell and can move along the axial direction, an inlet is formed between the upper end of the adjusting cone and the upper end of the shell, and an outlet is formed between the lower end of the adjusting cone and the lower end of the shell.

The fine adjustment assembly comprises an adjusting screw rod, a nut block, a support upright post and a transverse cantilever, the support upright post is fixed on the bearing plate, and a sliding groove is processed on the side wall of the support upright post; the cantilever is fixedly arranged at the upper end of the support upright post, and the upper end of the adjusting screw rod is rotatably arranged on the cantilever; the lower end of the nut block is fixed at the central position of the upper part of the adjusting cone, and the upper end of the nut block is in threaded connection with the adjusting screw rod; the lateral wall of nut piece passes through slider sliding fit and installs in the spout on the support post.

Further, still include the support, the support fixed mounting is on the outer wall of casing.

Further, the size of the gap of the outlet is 10-40 um.

Compared with the prior art, the invention has the following beneficial effects:

1. the adjusting cone is moved along the axial direction relative to the shell through the adjusting screw rod, so that the gap size of the outlet is adjusted within the range of 10-40 um. The human body liquid sample flows in from the inlet of the shell, circulating tumor cells can be sorted step by step due to the limitation of the fluid space, and the circulating tumor cells are sorted out from the outlet, so that the research on the circulating tumor cells is facilitated;

2. the design of the fine adjustment assembly is matched with the nut block through the adjusting screw rod, so that the accuracy is high, and the operation is convenient;

3. the invention has simple structure, low manufacturing cost and convenient operation and use.

Drawings

FIG. 1 is a schematic view of the overall structure of the progressive separation apparatus according to the present invention;

FIG. 2 is a side view of the progressive separation apparatus of the present invention;

in the figure: 1. the device comprises a shell, 2, an adjusting cone, 3, a support, 4, an outlet, 5, an adjusting screw rod, 6, a nut block, 7, a supporting upright post, 8 and a cantilever.

Detailed Description

The invention provides a device for separating circulating tumor cells step by step, which mainly comprises a conical shell 1, an adjusting cone 2, a fine adjustment assembly and a bracket 3, as shown in figures 1-2. The upper end diameter of casing 1 is greater than the lower extreme diameter, and the outer wall cooperation support 3 of casing 1 can be steady place on the plane. The adjusting cone 2 is positioned in the shell 1 and distributed coaxially, an outlet 4 is formed by a gap between the lower end of the adjusting cone 2 and the lower end of the shell 1, and an inlet is formed between the upper end of the adjusting cone 2 and the upper end of the shell 1. The fine tuning assembly is arranged at the upper end of the shell 1 through a bearing plate and comprises an adjusting screw rod 5, a nut block 6, a supporting upright post 7 and a transverse cantilever 8, the supporting upright post 7 is fixed on the bearing plate, and a sliding groove is processed on the side wall of the supporting upright post 7. Cantilever 8 fixed mounting is in the upper end of support post 7, and adjusting screw 5's upper end is rotatory to be installed on cantilever 8. The lower end of the nut block 6 is fixed at the central position of the upper part of the adjusting cone 2, and the upper end is in threaded connection with the adjusting screw rod 5. The lateral wall of the nut block 6 is installed in the sliding groove on the supporting upright post 7 in a sliding fit manner through the sliding block, so that the adjusting cone 2 can move up and down by rotating the adjusting screw rod 5, and the size of the gap of the outlet 4 is finely adjusted within the range of 10-40um by controlling the gap between the lower end of the adjusting cone 2 and the lower end of the shell 1, thereby realizing the step-by-step separation of circulating tumor cells.

Taking the separation of tumor cells in the range of 10-20um and tumor cells in the range of 20-30um as an example, the circulating tumor cell progressive separation device provided by the invention has the following specific use method:

1. firstly, the adjusting cone is moved by the adjusting screw rod, the gap of the outlet is 10um, a human body liquid sample flows in from the inlet, and cells with the inner diameter smaller than 10um flow out and are collected due to the limitation of the fluid space;

2. a vessel for collecting cells is arranged below the outlet, the adjusting cone is moved by the adjusting screw rod, the gap of the outlet is 20um, and 10-20um cells in the liquid flow out and are collected into the vessel;

3. another vessel for collecting cells is placed below the outlet, the adjusting cone is moved by the adjusting screw rod, the gap of the outlet is 30um, and 20-30um cells in the liquid flow out and are collected in the vessel.

By the method, tumor cells in the range of 10-20um and tumor cells in the range of 20-30um can be respectively separated and collected for special research.

Claims

1. The device for separating the circulating tumor cells step by step is characterized by comprising a conical shell (1) and an adjusting cone (2), wherein the adjusting cone is positioned in the shell and can move along the axial direction, an inlet is formed between the upper end of the adjusting cone (2) and the upper end of the shell (1), and an outlet (4) is formed between the lower end of the adjusting cone (2) and the lower end of the shell (1).

2. The progressive separation device according to claim 1, further comprising a fine adjustment assembly mounted at the upper end of the housing (1) through a bearing plate, wherein the fine adjustment assembly comprises an adjusting screw (5), a nut block (6), a support column (7) and a transverse cantilever (8), the support column (7) is fixed on the bearing plate, and a sliding groove is processed on the side wall of the support column (7); the cantilever (8) is fixedly arranged at the upper end of the support upright post (7), and the upper end of the adjusting screw rod (5) is rotatably arranged on the cantilever (8); the lower end of the nut block (6) is fixed at the central position of the upper part of the adjusting cone (2), and the upper end of the nut block is in threaded connection with the adjusting screw rod (5); the side wall of the nut block (6) is installed in a sliding groove in the supporting upright post (7) in a sliding fit mode through a sliding block.

3. Progressive separation device according to claim 1, further comprising a bracket (3), the bracket (3) being fixedly mounted on an outer wall of the casing (1).

4. A progressive separation device according to any one of claims 1 to 3 wherein the size of the gap at the outlet is 10 to 40 um.