CN220371064U - Microfluidic device and cell enrichment and dyeing integrated equipment - Google Patents
Microfluidic device and cell enrichment and dyeing integrated equipment Download PDFInfo
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- CN220371064U CN220371064U CN202321916277.XU CN202321916277U CN220371064U CN 220371064 U CN220371064 U CN 220371064U CN 202321916277 U CN202321916277 U CN 202321916277U CN 220371064 U CN220371064 U CN 220371064U
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- 238000009825 accumulation Methods 0.000 claims abstract description 31
- 238000007599 discharging Methods 0.000 claims description 8
- 238000004891 communication Methods 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 238000010186 staining Methods 0.000 claims description 2
- 241000521257 Hydrops Species 0.000 abstract description 11
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- 230000035508 accumulation Effects 0.000 description 16
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a microfluidic device and integrated cell enrichment and dyeing equipment, wherein the microfluidic device comprises: liquid accumulation lining table, filtering component and lining table net. The inner part of the hydrops lining table is of a funnel-shaped structure, and a flow guide strip protruding from the surface of the funnel-shaped structure is arranged for guiding the filtrate; the filtering component is arranged above the liquid accumulation lining table and is used for filtering a liquid sample; the lining table net is arranged at one end of the liquid accumulation lining table, one side of the lining table net is contacted with the filtering component, and the other side of the lining table net is contacted with the liquid accumulation lining table and is used for supporting the filtering component; and a diversion cavity is formed between the effusion lining table and the lining table net. According to the utility model, the lining table net, the flow guide strips arranged on the effusion lining table and the flow guide cavity formed between the lining table net and the effusion lining table are arranged below the filtering component, so that the filtrate can quickly pass through the flow guide cavity.
Description
Technical Field
The utility model belongs to the technical field of integrated cell enrichment and dyeing equipment, and particularly relates to a microfluidic device and integrated cell enrichment and dyeing equipment.
Background
The pharmaceutical industry and the biomedical engineering industry are two main posts of modern medicine industry, the biomedical industry consists of biotechnology industry and medicine industry, the biomedical engineering is the principle and method of comprehensive application life science and engineering science, the structure, the function and other life phenomena of human body are known in multiple levels in molecules, cells, tissues, organs and even the whole human body system from engineering perspective, and the total name of artificial materials, products, devices and system technology for disease prevention, disease treatment, human body function assistance and health care is researched.
In recent years, in the field of biomedical engineering, several emerging tumor diagnostic detection techniques, such as detection methods for circulating tumor DNA, circulating Tumor Cells (CTCs), known as liquid biopsies, have emerged. According to researches, the existence of the circulating tumor cells can be found in peripheral blood before solid tumor formation, so that the circulating tumor cell detection is very suitable for early screening and early diagnosis of malignant tumors, and has good effects on prognosis of malignant tumors, disease progress monitoring, recurrence prediction, postoperative tiny focus monitoring of malignant tumors and design and treatment effect monitoring of targeted drug treatment, and is an advanced method for early screening and diagnosis of malignant tumors at present.
In the detection of circulating tumor cells, filtration of blood samples is a common experimental means. The current use and operation of filtering devices are more important to the filtering of the obtained experimental samples, and the diversion and collection of the filtrate produced after the filtering are not important. In the process of filtering a blood sample, the filtered filtrate is often discharged out of the filtering device in time, so that the filtered filtrate is accumulated in the filtering device, the working efficiency of the filtering device is reduced, the quality of an experimental sample is poor, and the detection of relevant circulating tumor cells is adversely affected.
In addition, if a microfluidic device capable of effectively enriching target substances is designed, the microfluidic device plays an important role in various detection and research in the biomedical field. For example, in immunological research, genetic research or microbial detection, the efficiency of sorting target cells, organisms or biomacromolecules can be improved, and further, the research and development of biochips and the research and development of organoids are facilitated.
The present utility model has been made in view of this.
Disclosure of Invention
The utility model aims to overcome the defects of the prior art and provides a microfluidic device and cell enrichment dyeing integrated equipment, wherein a lining table net and a flow guide strip protruding from the inner surface of a funnel-shaped structure on a hydrops lining table are arranged below a filtering component, and a flow guide cavity is formed between the lining table net and the hydrops lining table, so that a filtrate produced after filtering can quickly pass through the flow guide cavity.
In order to solve the technical problems, the utility model adopts the basic conception of the technical scheme that: a microfluidic device, comprising:
the inner part of the effusion lining table is of a funnel-shaped structure and is used for collecting filtrate;
the filtering component is arranged above the liquid accumulation lining table and is used for filtering the liquid sample;
the lining table net is arranged at one end of the liquid accumulation lining table, one side of the lining table net is contacted with the filtering component, and the other side of the lining table net is contacted with the liquid accumulation lining table and is used for supporting the filtering component;
and a diversion cavity is formed between the effusion lining table and the lining table net and is used for diversion of the filtrate.
Further, a guide strip is arranged at one end of the hydrops lining table, which is close to the lining table net, and is arranged at the bottom of the guide cavity, the guide strip is in a rod shape, protrudes from the surface of the funnel-shaped structure, and gradually increases in height along the direction from the edge of the funnel-shaped structure inside the hydrops lining table to the center.
Preferably, the guide strips are annularly arranged on the surface of the funnel-shaped structure and are used for guiding the filtrate flowing through the lining table net so that the filtrate can quickly pass through the guide cavity.
Further, the surface of the lining table net is provided with a strip-shaped opening, the flow guide strip is at least partially arranged in the strip-shaped opening, and the strip-shaped opening is used for fixing the lining table net on the flow guide strip.
Preferably, the strip openings are distributed uniformly on the surface of the backing net in a ring shape, and at least two lengths of strip openings are provided for preventing the flow guide strips from completely blocking the strip openings, so that enough flowing space of the filtrate is ensured to pass through the backing net.
Further, one end of the hydrops lining table, which is close to the lining table net, is provided with an annular protruding structure, and the protruding structure is used for being clamped with the lining table net.
Preferably, the filter assembly at least partially enters a central cylindrical space defined by the projection structure, and is used for radially fixing the filter assembly to avoid rotation of the filter assembly during installation.
Furthermore, the hydrops lining table is also provided with a diversion hole, and the diversion hole is arranged at the bottom of the diversion cavity and is used for discharging the filtrate out of the diversion cavity.
Furthermore, the hydrops lining table is also provided with a diversion frustum which is arranged below the diversion cavity and communicated with the diversion hole, and the diversion frustum is used for converging and discharging the filtrate.
Furthermore, the microfluidic device of the integrated cell enrichment and dyeing equipment further comprises a communicating bottom pipe, wherein the communicating bottom pipe is arranged below the diversion cone, is communicated with the diversion cone and is used for discharging filtrate.
Further, the microfluidic device of the integrated cell enrichment and dyeing equipment further comprises a sample feeding pipe, one end of the sample feeding pipe is provided with a buckling part protruding from the side wall of the sample feeding pipe, and the inner space of the buckling part is used for installing the filter assembly.
Preferably, the outer diameter of the lower annular edge of the buckling part is not larger than the outer diameter of the protruding structure, so as to avoid leakage of the liquid sample from the edge of the protruding structure.
Further, the microfluidic device of the integrated cell enrichment and dyeing equipment further comprises a buckle cover, the buckle cover is in threaded connection with the liquid accumulation lining table, a limiting groove is formed in the buckle cover, the inner surface of the limiting groove is in contact with the outer surface of the buckling part, and the sample inlet pipe and the filtering component are tightly pressed on the top of the liquid accumulation lining table.
An integrated cell enrichment and staining device is provided with a microfluidic device.
After the technical scheme is adopted, compared with the prior art, the utility model has the following beneficial effects:
the utility model provides a microfluidic device and cell enrichment dyeing integrated equipment, which are characterized in that a lining table net and a flow guide strip protruding from the inner surface of a funnel-shaped structure on a hydrops lining table are arranged below a filtering component, and a flow guide cavity is formed between the lining table net and the hydrops lining table, so that filtrate produced after filtering can quickly pass through the flow guide cavity.
The following describes the embodiments of the present utility model in further detail with reference to the accompanying drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the utility model. It is evident that the drawings in the following description are only examples, from which other drawings can be obtained by a person skilled in the art without the inventive effort. In the drawings:
FIG. 1 is an exploded schematic view of a microfluidic device according to an embodiment of the present utility model;
FIG. 2 is a schematic view of the appearance of a microfluidic device according to the embodiment of the present utility model;
FIG. 3 is a cross-sectional view of a liquid product liner station according to an embodiment of the utility model;
FIG. 4 is a top view of a liquid product liner station according to an embodiment of the utility model;
FIG. 5 is a top view of a backing web according to an embodiment of the utility model;
FIG. 6 is a schematic view in the direction A of a front view of a buckle closure for connecting a bottom pipe with a rubber hose according to the embodiment of the present utility model;
FIG. 7 is a top view of a communication bottom tube according to the embodiment of the present utility model;
FIG. 8 is a cross-sectional view of a sample injection tube according to an embodiment of the present utility model;
FIG. 9 is a top view of a sample injection tube according to an embodiment of the present utility model;
FIG. 10 is a cross-sectional view of a buckle cover according to the embodiment of the present utility model;
FIG. 11 is a schematic view of the buckle closure according to the embodiment of the present utility model;
FIG. 12 is a front view of the embodiment of the utility model in communication with a base pipe and stationary table mounting location;
FIG. 13 is a top view of the mounting position of the connecting bottom tube and the stationary table according to the embodiment of the present utility model.
In the figure: 201. a sample inlet tube; 2012. a buckling part; 202. a buckle cover; 203. a liquid accumulation lining table; 2031. a threaded portion; 2032. an operation unit; 205. a backing net; 2051. a strip-shaped opening; 206. a flow guiding strip; 207. a limit groove; 208. a diversion frustum; 209. a communicating bottom pipe; 210. a fixed table; 211. a rubber hose; 212. microfiltration membrane; 213. a water-absorbing layer; 214. a filter assembly; 215. and a deflector hole.
It should be noted that these drawings and the written description are not intended to limit the scope of the inventive concept in any way, but to illustrate the inventive concept to those skilled in the art by referring to the specific embodiments.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions in the embodiments will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present utility model, and the following embodiments are used to illustrate the present utility model, but are not intended to limit the scope of the present utility model.
In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
In medical and biological experiments, filtering liquid samples is a common experimental means, and the use and operation of filtering equipment are more important to the filtering of the obtained experimental samples, and the diversion, collection and the like of filtrate generated after the filtering are not important. In the experimental process, the filtered filtrate is often discharged out of the filtering device in time, so that the filtered filtrate is accumulated in the filtering device, the working efficiency of the filtering device is reduced, the quality of experimental samples is poor, and the related experiments and researches are adversely affected. In order to solve the problem, the utility model provides a microfluidic device and a cell enrichment and dyeing integrated device, wherein a lining table net and a flow guide strip protruding from the inner surface of a funnel-shaped structure on a effusion lining table are arranged below a filtering component, and a flow guide cavity is formed between the lining table net and the effusion lining table, so that a filtrate produced after filtering can quickly pass through the flow guide cavity, and the lining table net can also support the filtering component. The method comprises the following steps:
as shown in fig. 1 to 13, the present utility model provides a microfluidic device comprising: liquid product backing 203, filter assembly 214, backing web 205. The effusion lining stand 203 comprises a screw thread part 2031, an operation part 2032, a diversion hole 215 and a diversion frustum 208; the screw 2031 is provided integrally with the operation portion 2032; the guide strips 206 are arranged on the surface of the funnel-shaped structure of the hydrops lining table 203; the diversion hole 215 is communicated with the diversion frustum 208 and is arranged in the effusion lining table 203; the filter assembly 214 includes a microfiltration membrane 212 and a water absorbing layer 213; the backing net 205 is disposed on top of the liquid accumulation backing 203, and the surface of the backing net 205 is provided with an elongated opening 2051.
As an embodiment, the present utility model provides a microfluidic device, as shown in fig. 1 to 13, comprising: liquid product backing 203, filter assembly 214, backing web 205.
The inner part of the effusion lining table 203 is in a funnel-shaped structure and is used for collecting filtrate;
the filtering component 214 is arranged above the liquid accumulation lining table 203 and is used for filtering a liquid sample;
the backing net 205 is disposed at one end of the liquid accumulation backing 203, one side of the backing net 205 contacts with the filter assembly 214, and the other side contacts with the liquid accumulation backing 203, for supporting the filter assembly 214;
a diversion cavity is formed between the effusion lining table 203 and the lining table net 205, and the diversion cavity is used for diversion of the filtrate.
In the above solution, the shape of the flow guiding cavity is in a shape of a reverse cone, and the flow guiding cavity is disposed above the funnel-shaped structure inside the effusion lining table 203, and the filtrate flows along the inner surface of the funnel-shaped structure and gradually converges at the bottom of the flow guiding cavity after entering the flow guiding cavity, so as to guide the filtrate out of the flow guiding cavity.
As shown in fig. 3, a flow guiding strip 206 is disposed at one end of the liquid accumulation lining table 203 near the lining table mesh 205, the flow guiding strip 206 is disposed at the bottom of the flow guiding cavity, and the flow guiding strip 206 is rod-shaped, protrudes from the surface of the funnel-shaped structure, and gradually increases in height along the edge of the funnel-shaped structure inside the liquid accumulation lining table 203 toward the center.
As shown in fig. 4, the flow guiding strips 206 are arranged in a ring shape on the surface of the funnel-shaped structure, and are used for guiding the filtrate flowing through the backing net 205, so that the filtrate can quickly pass through the flow guiding cavity.
It should be noted that the surfaces of the funnel-shaped structure and the guide strips 206 that contact the filtrate are smooth, do not adhere to the filtrate, and are not chemically reactive with the filtrate.
In the above solution, the height of the protrusion of the guide strip 206 along the edge of the inner funnel-shaped structure of the liquid accumulation lining table 203 towards the center is gradually increased, so that when the filtrate is less in a drop shape and enters the guide cavity, the drop-shaped filtrate drops and contacts with the guide strip 206, under the action of gravity and liquid tension, the filtrate flows on the surfaces of the guide strip 206 and the funnel-shaped structure along the direction of gradually increasing the protrusion height of the guide strip 206, and finally flows to the lowest position of the funnel-shaped structure.
As shown in fig. 5, the surface of the backing net 205 is provided with elongated openings 2051, and the guide strips 206 are at least partially disposed inside the elongated openings 2051, and the elongated openings 2051 are used to ensure that the filtrate can pass through the backing net 205 and to fix the backing net 205 to the guide strips 206.
In the above solution, the elongated opening 2051 contacts the bottom of the filter assembly 214 to increase the friction between the backing net 205 and the filter assembly 214, so that the filter assembly 214 is not easy to rotate relative to the backing net 205.
Preferably, the filter assembly 214 includes a microfiltration membrane 212, the microfiltration membrane 212 being used to filter circulating tumor cells in the liquid sample; the filter assembly 214 further includes a water-absorbing layer 213 disposed below the micro-filtration membrane 212, for maintaining the posture of the micro-filtration membrane 212 and rapidly absorbing the filtered sample filtrate or staining solution.
The end of the effusion lining table 203, which is close to the lining table net 205, is provided with an annular protruding structure, and the protruding structure is used for being clamped with the lining table net 205.
It should be noted that, the height of the protruding structure is not less than the thickness of the backing net 205, so as to avoid unstable installation of the backing net 205 in the protruding structure, and also avoid easy sliding when the filter assembly 214 contacts with the upper surface of the backing net 205, which affects installation.
As shown in fig. 3, the liquid accumulation lining table 203 is further provided with a diversion hole 215, and the diversion hole 215 is disposed at the bottom of the diversion cavity and is used for discharging the filtrate out of the diversion cavity.
The effusion lining stand 203 is further provided with a diversion frustum 208, which is arranged below the diversion cavity and is communicated with the diversion hole 215, and the diversion frustum 208 is used for converging and discharging the filtrate.
It should be noted that, the inner wall of the flow guiding cone 208 extends vertically downward from the flow guiding hole 215 to form a first hollow circular tube structure, the inner wall of the flow guiding cone 208 extends toward the central axis of the first circular tube structure to form a circular plane, the inner wall of the flow guiding cone 208 continues to extend vertically downward at the center of the circular plane to form a second hollow circular tube structure, the outer diameter of the second circular tube structure is smaller than the inner diameter of the first circular tube structure, and the extension length of the second circular tube structure does not exceed the plane where the annular bottom of the liquid product lining table 203 is located.
As shown in fig. 6 to 7, the microfluidic device further includes a communicating bottom pipe 209 disposed below the flow guiding frustum 208 and in communication with the flow guiding frustum 208, and a rubber hose 211 is further disposed below the communicating bottom pipe 209 and used for discharging the filtrate.
As shown in fig. 8 to 9, the microfluidic device further includes a sample tube 201, one end of the sample tube 201 is provided with a fastening portion 2012 protruding from a side wall of the sample tube 201, and an inner space of the fastening portion 2012 is used for mounting the filter assembly 214.
As shown in fig. 10 to 11, the microfluidic device further includes a cover 202 screwed to the liquid accumulation substrate 203, a limit groove 207 is provided in the cover 202, and an inner surface of the limit groove 207 contacts with an outer surface of the fastening portion 2012, and the sample injection tube 201 and the filter assembly 214 are pressed against the top of the liquid accumulation substrate 203.
As shown in fig. 12 to 13, the microfluidic device further includes a fixing stage 210 for fixing the liquid-product liner stage 203.
The upper surface of the fixing table 210 is provided with a through hole, and the communicating bottom pipe 209 is inserted into the through hole.
The integrated cell enrichment and dyeing equipment comprises the microfluidic device, a reagent taking and placing device and a blood sample adding device, wherein the reagent taking and placing device is used for adding reagents required for filtering and enriching cells into the microfluidic device, and the blood sample adding device is used for sending blood samples of a patient into the microfluidic device, so that cells to be detected are obtained after the filtration is completed. The reagent taking and placing device, the blood sample adding device and the microfluidic device are all arranged on the same fixed frame to form an integrated device for enriching and dyeing cells.
The foregoing description is only a preferred embodiment of the present utility model, and the present utility model is not limited to the above-mentioned embodiment, but is not limited to the above-mentioned embodiment, and any simple modification, equivalent change and modification made by the technical matter of the present utility model can be further combined or replaced by the equivalent embodiment without departing from the scope of the technical solution of the present utility model.
Claims (10)
1. A microfluidic device, comprising:
a liquid accumulation lining table (203) with a funnel-shaped structure inside for collecting filtrate;
a filter assembly (214) arranged above the liquid accumulation lining table (203) and used for filtering the liquid sample;
a backing net (205) arranged at one end of the liquid accumulation backing table (203), wherein one side of the backing net (205) is contacted with the filter assembly (214), and the other side is contacted with the liquid accumulation backing table (203) for supporting the filter assembly (214);
a diversion cavity is formed between the effusion lining table (203) and the lining table net (205), and the diversion cavity is used for diversion of filtrate.
2. The microfluidic device according to claim 1, wherein a flow guiding strip (206) is arranged at one end of the liquid accumulation lining table (203) close to the lining table net (205), and is arranged at the bottom of the flow guiding cavity, and the flow guiding strip (206) is in a rod shape, protrudes from the surface of the funnel-shaped structure, and gradually increases in height along the direction from the edge of the funnel-shaped structure inside the liquid accumulation lining table (203) to the center.
3. A microfluidic device according to claim 2, wherein the surface of the backing web (205) is provided with elongated openings (2051), the flow strips (206) being at least partially arranged inside the elongated openings (2051), the elongated openings (2051) being used for fixing the backing web (205) to the flow strips (206).
4. A microfluidic device according to claim 3, wherein the end of the liquid accumulation backing (203) adjacent to the backing web (205) is provided with an annular protrusion structure for clamping the backing web (205).
5. The microfluidic device according to claim 4, wherein the liquid accumulation backing (203) is further provided with a diversion hole (215), and the diversion hole (215) is arranged at the bottom of the diversion cavity and is used for discharging the filtrate out of the diversion cavity.
6. The microfluidic device according to claim 5, wherein the liquid accumulation lining table (203) is further provided with a diversion frustum (208) which is arranged below the diversion cavity and is communicated with the diversion hole (215), and the diversion frustum (208) is used for converging and discharging the filtrate.
7. The microfluidic device according to claim 6, further comprising a communication bottom tube (209) disposed below the flow cone (208), in communication with the flow cone (208), for draining filtrate.
8. The microfluidic device according to any one of claims 1-7, further comprising a sample tube (201), wherein one end of the sample tube (201) is provided with a buckling part (2012) protruding from a side wall of the sample tube (201), and an inner space of the buckling part (2012) is used for mounting the filter assembly (214).
9. The microfluidic device according to claim 8, further comprising a buckle cover (202) in threaded connection with the liquid accumulation lining table (203), wherein a limit groove (207) is formed in the buckle cover (202), an inner surface of the limit groove (207) is in contact with an outer surface of the buckling part (2012), and the sample injection tube (201) and the filter assembly (214) are pressed on top of the liquid accumulation lining table (203).
10. A cell enrichment staining apparatus comprising a microfluidic device according to any of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321916277.XU CN220371064U (en) | 2023-07-20 | 2023-07-20 | Microfluidic device and cell enrichment and dyeing integrated equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321916277.XU CN220371064U (en) | 2023-07-20 | 2023-07-20 | Microfluidic device and cell enrichment and dyeing integrated equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220371064U true CN220371064U (en) | 2024-01-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321916277.XU Active CN220371064U (en) | 2023-07-20 | 2023-07-20 | Microfluidic device and cell enrichment and dyeing integrated equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220371064U (en) |
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- 2023-07-20 CN CN202321916277.XU patent/CN220371064U/en active Active
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