CN1110717A - Extracting polyse protein from - Google Patents
Extracting polyse protein from Download PDFInfo
- Publication number
- CN1110717A CN1110717A CN 94115876 CN94115876A CN1110717A CN 1110717 A CN1110717 A CN 1110717A CN 94115876 CN94115876 CN 94115876 CN 94115876 A CN94115876 A CN 94115876A CN 1110717 A CN1110717 A CN 1110717A
- Authority
- CN
- China
- Prior art keywords
- prozyme
- protein
- technique according
- consumption
- accounts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Polyose protein is extracted from champignon by use of compound enzyme that is prepared with pectase, cellulase, amylase, hemicellulase, protease and glucosidase through such technological steps as adding the prepared compound enzyme in cooled lixiviated liquid, enzymlysis, deactivating and concentration. The polyose matter is dried by spraying and then loaded in capsules. Its advantages include high content of active components and extraction rate up to 80%, and strong physiologic and immune activities.
Description
The present invention relates to a kind of novel method of polysaccharide protein in the prozyme extraction mushroom and polysaccharide protein that utilization extracts of utilizing and make the capsular technology of lentinan protein
Mushroom [Lenlinus edodes (Berk) sing] belongs to the fungi of Basidiomycetes Agaricaceae one class, originates from China, and cultivation and edible Xianggu-mushroom become a kind of popular food in China's history of existing nearly one thousand years.Contain nearly 65% carbohydrate in the dried thin mushroom (cap and mushroom handle), wherein have part to exist, also contain a certain amount of protein, VITAMIN, organic acid, trace element simultaneously with the polysaccharide form.Modern biology and medical research show that mushroom has nutrition and edible, also contains multiple active medicinal matter except self, as lentinan and protein polymer thereof.That such material has is antiviral, antitumor, strengthen body immunity and stimulate multiple functions such as Interferon, rabbit formation, be particularly suitable for the treatment of patient after immunologic hypofunction, cancer return and the chemotherapy, be a kind of immunological adjuvant without any side effects, be one of present known best immunopotentiating agent.
For this reason, the extracts active ingredients of lentinan has been carried out extensive studies both at home and abroad.In the 60 to 70's, extensively carried out lentinan based on many countries of Japan and extracted and drug efficacy study.Nineteen sixty-eight, the doctor of medicine thousand former Wu youths at Japanese state-run tumor research center utilize hot water at first from mushroom fruiting body lixiviate go out lentinan outside 6 kinds of born of the same parents, wherein 2 kinds have obvious antineoplastic; 1970, the Chihard and the SaSaki of Japan also adopted hot water lixiviate, the isolating method of organic solvent deposit to extract four kinds of polysaccharide materials from mushroom; Before 1976 the sub-comprehensive dispatch of good fortune the polysaccharide material that arrives with the similar approach extracting; People such as Shida utilized the trichoroacetic acid(TCA) lixiviate in 1971, and intermediate processings such as methyl alcohol extract other three kinds of water-soluble polysaccharide materials from mushroom.Enter the eighties, the extraction of lentinan and the research in source have been strengthened in countries in the world.Discovery is merely hit from the mushroom mycelium of submerged fermentation and can be extracted intracellular polyse as Schully, and also there is similar report in China Henan medical professionals institute; U.S.'s report in 1984 is cultivated mushroom with the solid medium that is rich in wood sugar, and can use as above affiliated similar approach to extract from the mycelium culture obtain a kind of be the polysaccharide protein of main composition with wood sugar.
In sum, the separation and Extraction of lentinan and purifying thereof mainly adopt the method for hot water lixiviate in the prior art, and big to process: processes such as boiling water extraction, ethanol sedimentation, dialysis and column chromatography obtain the raw product of polysaccharide protein, extract yield below 10%.Also have the method for report in recent years with enzyme-added isolating protein, as with the polysaccharide raw product through processing such as streptomyces proteinase, stomach en-, papoid or trypsinase, through dialysis, can there be the lentinan mixture of protein and small molecular weight impurity so substantially again.
Though adopt the hot water leach extraction method extracting to go out multiple lentinan, it is very numerous and diverse to extract the shunting process, used alcohol and soda acid liquid measure are big, and the extraction yield of lentinan is low, generally can only reach 10%.Because the polysaccharide protein molecular structure that uses acid-base solution, lixiviate to go out wrecks to a certain extent relatively largely, cause protein-active to reduce, influence the drug effect of polysaccharide protein.
Task of the present invention provides a kind ofly to be utilized prozyme to extract the lentinan material and makes the capsular method of polysaccharide protein.This method can be extracted exocellular polysaccharide, protein, amino acid in the born of the same parents simply efficiently, can keep the biological activity of extract again.
Task of the present invention is finished with following scheme: mushroom fruiting body (comprising mushroom lid and the mushroom handle) lapping powder of getting oven dry is broken into the above mushroom dry powder of 30 orders, add the water of 7 times of amounts and be heated to 85～93 ℃ of backs that stir and kept this temperature 60 minutes, be cooled to 40～45 ℃, add the prozyme liquid for preparing in advance, add-on accounts for 3～7% of mushroom dry powder amount, and optimal addn is 5%.The back that stirs keeps this temperature to carry out enzymolysis in 60 minutes, is warming up to 90～100 ℃ then rapidly and carries out deactivation in 1 minute, the cooling after-filtration, concentrates, and 70 ℃ are dried to dry product, incapsulate, and can obtain the lentinan protein capsule product.Prozyme is formulated in following ratio: polygalacturonase 65～85%, cellulase 3～8%, amylase 2～8%, hemicellulase 2～4%, proteolytic enzyme 2～8%, saccharifying enzyme 4～10%.
Technical scheme of the present invention is further specified by following examples
Embodiment one,
1. raw material is handled
Get mushroom body 1000 grams of having dried, grind to the mushroom powder more than 30 orders.
2. the preparation of prozyme
Get polygalacturonase 33.5 and restrain, cellulase 4.0 grams, amylase 2.5 grams, hemicellulase 2.5 grams, proteolytic enzyme 2.5 grams, it is stand-by that saccharifying enzyme 5 restrains the prozyme liquid that is mixed into 50 grammes per square metres.
3. hot water lixiviate
The mushroom powder of 1000 grams are added 7000 milliliters water, be heated to 85 ℃, the back that stirs keeps 85 ℃ to carry out the hot water lixiviate in 60 minutes.
4. enzymolysis, deactivation
The lixiviate mixed solution is cooled to 40 ℃ from 85 ℃, adds prozyme liquid 50 grams that prepare in advance, the back that stirs keeps 40 ℃ and carries out 60 fens clock time enzymolysis.Be cooled to 90 ℃ then rapidly and carry out quick inactivating, deactivation is the naturally cooling cooling after 1 minute.
5. centrifugal, concentrated
Utilize the frame whizzer, get rid of filter, remove slag with the 3000r.p.m rotating speed; Filtrate concentrates 7～8 hours with film under vacuum.
6. dry, glue capsule
Under 70 ℃ of conditions, carry out spraying drying after adding appropriate amount of starch in the concentrated solution, obtain the polysaccharide protein dry product, in incapsulating, can obtain the capsule product of lentinan protein.
Embodiment two
1. raw material is handled
Get the mushroom body of having dried 1000 grams, grind to 30 orders and become the mushroom powder.
2. the preparation of prozyme
Get polygalacturonase 25.5 and restrain, cellulase 1.5 grams, amylase 0.5 gram, hemicellulase 0.5 gram, proteinase-10 .5 gram, it is stand-by that saccharifying enzyme 1.5 restrains the prozyme liquid that is mixed into 30 grammes per square metres.
3. hot water lixiviate
The water that adds 7000 milliliters in the mushroom powder of 1000 grams is heated to 93 ℃, and the back that stirs keeps 93 ℃ to carry out the hot water lixiviate in 60 minutes.
4. enzymolysis, deactivation
The lixiviate mixed solution is cooled to 45 ℃, prozyme liquid 30 grams that adding prepares in advance, 45 ℃ of enzymolysis that carry out 60 minutes of maintenance after stirring from 93 ℃.Be warming up to 100 ℃ then rapidly prozyme carried out quick inactivating, deactivation is the naturally cooling cooling after 1 minute.
5. centrifugal, concentrated
Utilize the frame whizzer, get rid of filter, remove slag with the 3000r.p.m. rotating speed; Filtrate concentrates 7～8 hours with film under vacuum.
6. dry, glue capsule carry out spraying drying after the adding appropriate amount of starch in the concentrated solution under 70 ℃ of conditions, obtain the polysaccharide protein dry product, in incapsulating, can obtain the capsule product of lentinan protein.
Embodiment three
1. raw material is handled and to be got mushroom body 1000 grams of having dried, grinds to 30 orders to become the mushroom powder.
2. the preparation of prozyme
Get polygalacturonase 59 gram, cellulase 2.0 grams, amylase 1.5 grams, hemicellulase 3.5 grams, proteolytic enzyme 40 grams, it is stand-by that saccharifying enzyme 2.0 grams are mixed into the prozyme liquid of 70 grammes per square metres.
3. hot water lixiviate
The water that adds 7000 milliliters in the mushroom powder of 1000 grams is heated to 90 ℃, and the back that stirs keeps 90 ℃ to carry out the hot water lixiviate in 60 minutes.
4. enzymolysis deactivation
The lixiviate mixed solution is cooled to 43 ℃, prozyme liquid 70 grams that adding prepares in advance, 43 ℃ of enzymolysis that carry out 60 minutes of maintenance after stirring from 90 ℃.Be warming up to 95 ℃ then rapidly prozyme carried out quick inactivating, deactivation is the naturally cooling cooling after 1 minute.
5. centrifugal, concentrated
Utilize the frame whizzer, get rid of filter, remove slag with the 300r.p.m rotating speed; Filtrate concentrates 7～8 hours with film under vacuum.
6. dry, glue capsule
Under 70 ℃ of conditions, carry out spraying drying after adding appropriate amount of starch in the concentrated solution, obtain the dry product of lentinan after, incapsulate, can obtain the capsule product of lentinan protein.
Extractive technique of the present invention, effective constituent-polysaccharide that is extracted and protein etc., its content height, extraction efficiency reaches more than 80%, more especially the sulfur-containing amino acid amplification that has antineoplastic polysaccharide material and needed by human is obvious especially, also kept simultaneously the solid and the optically-active structure of extract to greatest extent, made it have extremely strong physiologically active and immunocompetence.
Claims (10)
1, utilize mushroom fruiting body (comprising mushroom lid and mushroom handle) to be raw material, through grinding, heat lixiviate, the concentrated technology of centrifuging, it is characterized in that this technology adds a certain amount of prozyme that is mixed with by polygalacturonase, cellulase, amylase, hemicellulase, proteolytic enzyme and saccharifying enzyme and carries out enzymolysis after the heating lixiviate under 40-45 ℃ condition with the extraction lentinan protein.
2, extractive technique according to claim 1 is characterized in that the consumption of prozyme accounts for 3～7% of grinding back mushroom dry powder amount, and optimum amount is 5%.
3, extractive technique according to claim 1 is characterized in that adding the enzymolysis that carried out behind the prozyme 60 minutes, is warming up to 90～100 ℃ of deactivations of carrying out 1 minute then rapidly.
4, extractive technique according to claim 1 is characterized in that the consumption of polygalacturonase accounts for 65～85% of prozyme weight.
5, extractive technique according to claim 1 is characterized in that the consumption of cellulase accounts for 3～8% of prozyme weight.
6, extractive technique according to claim 1 is characterized in that diastatic consumption accounts for 2～8% of prozyme weight.
7, extractive technique according to claim 1 is characterized in that the consumption of hemicellulase accounts for 2～4% of prozyme weight.
8, extractive technique according to claim 1 is characterized in that the consumption of proteolytic enzyme accounts for 2～8% of prozyme weight.
9, extractive technique according to claim 1 is characterized in that the consumption of saccharifying enzyme accounts for 4～10% of prozyme weight.
10, utilize the technology of the described extraction lentinan protein of claim 1, it is characterized in that being spray dried to dry product after the polysaccharide protein that extracts adds an amount of starch, incapsulate and make the lentinan protein capsule product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94115876A CN1045472C (en) | 1994-09-15 | 1994-09-15 | Extracting polyse protein from |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94115876A CN1045472C (en) | 1994-09-15 | 1994-09-15 | Extracting polyse protein from |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1110717A true CN1110717A (en) | 1995-10-25 |
| CN1045472C CN1045472C (en) | 1999-10-06 |
Family
ID=5037690
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN94115876A Expired - Fee Related CN1045472C (en) | 1994-09-15 | 1994-09-15 | Extracting polyse protein from |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1045472C (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095471C (en) * | 1998-11-06 | 2002-12-04 | 陈哲超 | Method for processing lentinan protein |
| CN101828686A (en) * | 2010-04-26 | 2010-09-15 | 浙江温州轻工研究院 | Mushroom seasoning juice and preparation method thereof |
| CN102242100A (en) * | 2011-04-20 | 2011-11-16 | 威海康博尔生物药业有限公司 | Method for using compound biological enzyme in plant extraction process, and conditions thereof |
| CN104231109A (en) * | 2014-10-20 | 2014-12-24 | 哈尔滨派特纳生物科技开发有限公司 | Method for extracting undaria pinnatifida polysaccharide |
| CN104856150A (en) * | 2015-04-26 | 2015-08-26 | 哈尔滨伟平科技开发有限公司 | Manufacturing method of lentinan health-care beverage |
| CN104922161A (en) * | 2015-06-01 | 2015-09-23 | 吉林农业大学 | Lentinus lepideus polysaccharide protein compound, and preparation method and application thereof |
| CN105063129A (en) * | 2015-08-07 | 2015-11-18 | 广西南宁派腾科技有限公司 | Ultrasonic extraction process of kudzuvine root fructose |
| CN106279446A (en) * | 2016-08-29 | 2017-01-04 | 唐汉军 | Starch, fibrous polysaccharaide and the method for water solublity isoflavone is extracted from Radix Puerariae |
| CN108783387A (en) * | 2017-05-04 | 2018-11-13 | 仲景食品股份有限公司 | A kind of mushroom zymolyte of the fresh flavor of natural mushroom |
| CN113106138A (en) * | 2021-03-16 | 2021-07-13 | 中国农业科学院农产品加工研究所 | Preparation method for extracting and separating anti-tumor active protein from shiitake mushrooms |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1028715C (en) * | 1989-12-01 | 1995-06-07 | 李全才 | Process for forced extraction of mushroom juice with enzyme preparation |
-
1994
- 1994-09-15 CN CN94115876A patent/CN1045472C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095471C (en) * | 1998-11-06 | 2002-12-04 | 陈哲超 | Method for processing lentinan protein |
| CN101828686A (en) * | 2010-04-26 | 2010-09-15 | 浙江温州轻工研究院 | Mushroom seasoning juice and preparation method thereof |
| CN101828686B (en) * | 2010-04-26 | 2012-09-05 | 浙江温州轻工研究院 | Mushroom seasoning juice and preparation method thereof |
| CN102242100A (en) * | 2011-04-20 | 2011-11-16 | 威海康博尔生物药业有限公司 | Method for using compound biological enzyme in plant extraction process, and conditions thereof |
| CN104231109A (en) * | 2014-10-20 | 2014-12-24 | 哈尔滨派特纳生物科技开发有限公司 | Method for extracting undaria pinnatifida polysaccharide |
| CN104856150A (en) * | 2015-04-26 | 2015-08-26 | 哈尔滨伟平科技开发有限公司 | Manufacturing method of lentinan health-care beverage |
| CN104922161A (en) * | 2015-06-01 | 2015-09-23 | 吉林农业大学 | Lentinus lepideus polysaccharide protein compound, and preparation method and application thereof |
| CN105063129A (en) * | 2015-08-07 | 2015-11-18 | 广西南宁派腾科技有限公司 | Ultrasonic extraction process of kudzuvine root fructose |
| CN106279446A (en) * | 2016-08-29 | 2017-01-04 | 唐汉军 | Starch, fibrous polysaccharaide and the method for water solublity isoflavone is extracted from Radix Puerariae |
| CN108783387A (en) * | 2017-05-04 | 2018-11-13 | 仲景食品股份有限公司 | A kind of mushroom zymolyte of the fresh flavor of natural mushroom |
| CN113106138A (en) * | 2021-03-16 | 2021-07-13 | 中国农业科学院农产品加工研究所 | Preparation method for extracting and separating anti-tumor active protein from shiitake mushrooms |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1045472C (en) | 1999-10-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102847134B (en) | Preparation method for chlorella polypeptide microcapsule | |
| CN105111325B (en) | A kind of continuous method for extracting polysaccharide, protein and dietary fiber in pin from needle mushroom | |
| CN104232718B (en) | The preparation method and application of dendrobium candidum antineoplastic polypeptide | |
| CN102838686B (en) | Method for preparing high-purity agaricus blazei murrill polysaccharide | |
| CN1045472C (en) | Extracting polyse protein from | |
| CN101469338A (en) | Enzymolysis preparation of Cordyce militaris polypeptide and product | |
| WO2012013112A1 (en) | Method for extracting effective ingredients from sea cucumber by salting out | |
| CN103271951A (en) | Method for preparing cordyceps preparations with high adenosine contents | |
| CN107602719B (en) | Ganoderma lucidum fruiting body refined polysaccharide with significant auxiliary anti-tumor activity and preparation method and application thereof | |
| CN111004829A (en) | Preparation method of black fungus active peptide | |
| CN107119096B (en) | Preparation method and application of pholiota nameko active peptide | |
| CN113249421A (en) | Golden-silk-royal chrysanthemum protein polypeptide and preparation and application thereof | |
| CN104762356B (en) | A kind of pumpkin leaf polyose and polypeptide and preparation method thereof | |
| CN113106138A (en) | Preparation method for extracting and separating anti-tumor active protein from shiitake mushrooms | |
| CN109180829A (en) | A kind of preparation method and application of Pueraria Flavonid and polyoses extract | |
| CN111838673A (en) | Sunflower pollen extract and preparation method and application thereof | |
| CN1181208C (en) | Production method of oligose and beverage containing same | |
| CN1256318C (en) | Process for preparing chicoric acid from chicory | |
| CN114560959B (en) | Preparation method of mushroom extract | |
| CN116196281A (en) | Preparation method of water-soluble powder of cordyceps militaris extract | |
| CN109680030A (en) | A kind of inoxidizability coconut polypeptide and the preparation method and application thereof | |
| CN115381861B (en) | Extraction method and application of mushroom nutrient solution | |
| CN110881629A (en) | Red date zymolyte and preparation method and application thereof | |
| CN107973845B (en) | Preparation method of Guangxi golden camellia polysaccharide peptide | |
| CN1350861A (en) | Process for preparing health-care oral liquid to prevent cancer and delay sanility |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| C10 | Entry into substantive examination | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |