CN1256318C - Process for preparing chicoric acid from chicory - Google Patents

Abstract

The present invention provides a preparation process for extracting a high-content and high-purity chicoric acid product from chicory, comprising post-harvest treatment of chicory raw materials, increasing the content of chicoric acid in the harvested chicory, and significantly reducing the cost of raw materials; pre-treating the processed raw materials, preserving the chicoric acid in the plants to the maximum extent without being decomposed; selecting appropriate extraction solvents and separation technologies, extracting and separating active ingredients in the plants to the maximum extent, and reducing production costs; selecting scientifically advanced purification technologies and equipment, effectively removing non-chicoric acid impurities, and increasing the purity and content of chicoric acid; selecting optimized separation carriers and fillers, and realizing industrial production of pure chicoric acid products; selecting appropriate commodity existence forms, and extending the shelf life of chicoric acid. The method of the present invention can produce a variety of products to meet different needs for products.

Description

A kind of technology that utilizes chicory to prepare chicory acid

technical field

The invention belongs to the field of chemical extraction, in particular to a process for producing cichoric acid by using chicory.

Background technique

Chichoric acid, also known as two coffee tartaric acid, is a derivative of caffeic acid. It is an immunostimulator and immunomodulator that is widely valued in the world. In addition, it can stimulate phagocytosis, inhibit the replication of hyaluronidase, protect collagen III from free radicals that can cause degradation, and resist HIV, anti-cancer, anti-aging, etc., are the main ingredients used in pharmaceutical raw materials and health food.

Chichoric acid usually exists in Compositae plants. At present, the international production of cichoric acid is mainly extracted from Echinacea purpurea, Echinacea purpurea, and Echinacea chinensis. Extracting and preserving cichoric acid from plants is very challenging because of its chemical instability. The Chinese patent application number is CN00813200, titled "Echinacea tonic and preparation method", which provides a tonic with at least two components including cichoric acid and its preparation method, and the raw material used is Echinacea Chrysanthemum, the product is aimed at the extraction of various active ingredients. However, in the medicinal plants in our country, this component mainly exists in the plant Chicory. Chicory is a perennial herb of the genus Chicory in the Compositae family. It originates in Europe and is widely used as feed, vegetables and sugar raw materials. variety, widely cultivated.

Most cichoric acid currently on the market exists in the form of a liquid, which contains a large amount of water, and water increases the weight and volume of the cichoric acid product, increasing unnecessary processing and transportation costs. The water in fresh chicory juice provides an active environment for microorganisms and yeasts, making the chicory juice easy to ferment and mildew, and chicory acid to be easily degraded. Therefore, making cichoric acid into powder, tablets, and capsules has become a focus of attention of those skilled in the art to overcome the above problems.

Contents of the invention

The present invention aims to provide a process for extracting caffeic acid derivatives, which can promote health, from chicory, which is a bioactive ingredient that can promote health. cost.

The concrete technical scheme that realizes above-mentioned object is, 1. a kind of technology that utilizes chicory to prepare cichoric acid, is raw material with the root, stem, leaf of chicory, it is characterized in that, this technique comprises the following steps:

1) Post-harvest treatment of chicory

a. Chicory harvesting: choose chicory planted for 1-3 years, select the stems and leaves of the aboveground part as raw materials during the full flowering stage, and dig the roots as raw materials after the stems and leaves of the aboveground parts wither;

b. Crushing: Immediately pulverize the stems and leaves of the harvested aerial parts in a cold room at 4-10°C after removing impurities; quickly wash away the silt and sundries for the root raw materials, quickly air-dry and slice;

c. Control relative humidity, adjust oxygen, and low-temperature treatment: Put the crushed stem and leaf raw materials and sliced root raw materials into a polyethylene bag or burlap bag or paper bag, keep it open, and expose the raw materials to the air completely Among them, there are preferably several openings of 2-3cm on the bag, and then the bag is quickly put into the freezing environment, and its relative humidity is kept at 40-85%, the temperature is between 0-10°C, and the time is 96 hours.

The chicory raw material processed above is directly processed in the next process, or dried by microwave vacuum drying or freeze-sublimation drying, so that the moisture content in the raw material is less than 10%, and it is processed and used later;

2) Extraction step

The extraction is divided into the stem and leaf raw material extraction of the aerial part and the chicory root raw material extraction

a. Stem and leaf raw material extraction of aerial parts:

Add 40%-70% ethanol or methanol solution to the dried chicory raw material as the extraction solvent, the ratio of solid to liquid is 1: 3G/V～1: 20G/V and while stirring, the rate of feeding into nitrogen is 500L/hour to prevent Degradation of cichoric acid; standing at room temperature for 30 hours, then extracting cichoric acid to obtain a cichoric acid extract;

Or add 40%-70% ethanol or methanol solution to the chicory raw material as an extraction solvent, use ultrasonic extraction for 40 minutes, and the ratio of solid to liquid is 1:3G/V～1:10G/V; centrifuge or squeeze to obtain the chicory acid extract, and the residue is then extracted Repeat the extraction once, and the extracts are combined;

b. Raw material extraction of chicory root:

Grind the freeze-treated dried chicory root material again, add boiling water, the ratio of material to liquid is 1:2G/V～1:20G/V, extract in water for 2～4.5 minutes, cool to 60°C and place at this temperature for 4 hours, squeeze the juice through a mechanical screw press, and repeat the leaching of the obtained solid residue twice, and combine the chicory acid extract;

3) Prepare cichoric acid powders with different contents according to different product content requirements from the cichoric acid extract prepared in the above extraction step.

The above-mentioned cichoric acid powders with different contents include cichoric acid product A, cichoric acid product B, and cichoric acid product C, and their preparation methods are respectively:

a. prepare cichoric acid product A

The cichoric acid extract obtained by extracting the stems and leaves of the aerial part is filtered through a hollow inorganic fiber microfiltration membrane to remove proteins, tannins, polysaccharides and other large molecular weight interference components as well as impurities such as microorganisms, bacteria, viruses, etc., and the microfiltration membrane The pore size is between 0.10μm and 0.25μm, and then concentrated by a rotary thin film evaporator at 40°C, dried at 60°C and a vacuum of 0.08MPa to obtain cichoric acid powder. The content of the cichoric acid powder is higher than 30%, which is a cichoric acid product. A;

b. Preparation of Chichoric Acid Product B

①Refinement of chicory acid extract from chicory root raw material:

Concentrate the root chicory acid extract to less than 1/2 of the original extract, then add 1-5 times the amount of 95% alcohol, unit: V/V, after adding alcohol, the alcohol content of the solution is not less than 60%, Unit: V/V, let stand for 2-5 hours, suction filter or centrifuge to remove polysaccharides and other precipitated substances in the juice, and centrifuge to obtain juice with a higher concentration of cichoric acid. A hollow fiber inorganic ultrafiltration membrane with a pore size of 0.05-0.25 μm is preferred to completely remove bacteria, solidified and water-soluble proteins, colloids, pigments and other insoluble substances to obtain a refined extract of cichoric acid;

②Preparation of cichoric acid product B:

Concentrate the refined chicory acid extract under vacuum at 40-50°C until the solid content in the extract is between 20% and 35%, then heat to 80°C, add maltose or maltodextrin, phosphoric acid to the concentrate Tricalcium non-hygroscopic carrier, the ratio of the added amount to the soluble solid is 0.2:1~1.2:1, and then dried by vacuum drying and spray drying, and the inlet temperature is controlled between 149°C and 160°C during the spray drying method , the outlet temperature is controlled between 93°C and 104°C, and the obtained product is filtered through a 100-mesh sieve to ensure that at least 40% of it passes through; or at least 100% passes through a 16-mesh sieve to finally obtain a cichoric acid content of more than 20%. Chichoric acid powder is cichoric acid product B.

c. Preparation of Chichoric Acid Product C

①Macroporous adsorption resin adsorption separation:

Concentrate the above-mentioned cichoric acid refined extract obtained from the aerial parts or roots of chicory to remove alcohol, redissolve it with distilled water or deionized water, and put the sample on a macroporous adsorption resin column at a speed of 10ml/min, and let the resin fully stand Adsorption balance for more than 5 hours, wash with water to remove impurities that are not adsorbed by the resin, and then elute with 5%-10% methanol-water, unit: V/V, collect the eluate, concentrate with a rotary thin film evaporator at 40°C, and remove methanol , to obtain cichoric acid;

②C-18 reverse silica gel column chromatography:

The cichoric acid extract obtained from the purification of the macroporous adsorption resin is dissolved in methanol solution, put on a C-18 silica gel column, and eluted with 50% methanol solution, and developed with toluene:ethyl acetate:formic acid equal to 5:2:0.7 reagent, silica gel GF ₂₅₄ thin plate development, with cichoric acid standard product as a control, detection and combined collection of cichoric acid eluate, concentration, removal of methanol, water recrystallization, freeze-sublimation drying, to obtain pure cichoric acid with a content of more than 90%. That is the cichoric acid product c.

The cichoric acid prepared by the method of the present invention takes water and alcohol-water as the extracting solution, which reduces the production cost, improves the extraction rate, and avoids the decomposition of cichoric acid; the invention effectively removes non-cichoric acid components and prevents other components from affecting Interference with the activity and function of cichoric acid; and the cichoric acid prepared by the method of the invention is in powder form, which prevents fermentation mildew and degradation.

The present invention mainly uses ethanol and water to mix in a certain ratio or directly uses water as the extraction solvent to extract the cichoric acid, and the extraction is relatively thorough. In the process of extracting and stirring, nitrogen gas is introduced simultaneously to prevent the degradation of the cichoric acid, and the juice containing the cichoric acid is obtained. Membrane filtration or membrane filtration after alcohol precipitation can effectively remove proteins, tannins, polysaccharides and other large molecular weight interference components and impurities such as microorganisms, bacteria, viruses, etc., to obtain a refined cichoric acid extract. After concentrating and drying, the cichoric acid powder product is obtained through the following two different processes. One method is membrane-filtered juice to remove impurities, concentrated and dried to obtain cichoric acid powder with humidity less than 8%. Another method is to use alcohol precipitation to remove polysaccharides in the juice, and then concentrate the juice through membrane filtration to remove other impurities, and add maltose, tricalcium phosphate and other carriers during the concentration process, and pass through a sieve after drying to obtain high-purity Chichoric Acid Powder. At present, the content of active ingredients of cichoric acid products sold in the market is relatively low, the highest being no more than 4%. Through the above treatment, the content of cichoric acid in the product can be increased to more than 20%.

Chichoric acid is easy to decompose and has strong polarity. It is very difficult to industrially produce pure chichoric acid. In the present invention, the refined cichoric acid extract obtained above is adsorbed and separated by a macroporous adsorption resin, and then separated and purified by ODS reverse column chromatography to obtain Pure cichoric acid with a purity of over 90%.

Description of drawings

Fig. 1 is process extraction flowchart of the present invention;

Fig. 2 is the postharvest processing flowchart of chicory leaf;

Fig. 3 is the preparation flowchart of powdery cichoric acid product A;

Fig. 4 is the preparation flowchart of cichoric acid product B;

Figure 5 is a flow chart for the preparation of cichoric acid product C.

The process technology of the present invention will be further described in detail below in conjunction with the accompanying drawings and examples.

Detailed ways

In the present invention, at first select each organ of chicory to harvest when its cichoric acid content is the highest, and the chicory after harvest is pulverized immediately, then controls the relative humidity, and carries out post-harvest treatment measures such as low-temperature storage in a semi-aerobic state. Compared with the original chicory, the content of the active ingredient cichoric acid in the post-treated chicory is increased by at least 1%. This is because given suitable conditions, the chichoric acid enzyme in the plant continues to carry out physiological activities, allowing its secondary metabolism to continue and synthesize chichoric acid. However, after 96 hours, the catabolism of chichoric acid takes the dominant position, and the content of chichoric acid begins to decline. Proceed to the next process. This research has greatly reduced the cost of cultivation.

Chicory after the above treatment still contains a lot of water. If the plants are kept in a high humidity state, the cichoric acid will be significantly degraded in a short time. In order to facilitate the production and realize the long-term production of cichoric acid, the chicory that has been treated above needs to be dried immediately. For processing, the present invention adopts freeze-drying method, microwave vacuum drying method or blast drying method to effectively protect cichoric acid and avoid its degradation.

1. Post-harvest treatment of chicory:

The content of cichoric acid is affected by plant species, growth years and growth conditions. In order to produce high concentrations of cichoric acid, many factors must be considered, such as harvesting stage, production practices and extraction liquid.

1) Harvesting of chicory: Chicory is best selected to be planted for 1-3 years. After planting for more than 4 years, the content will decrease; in the full flowering stage, the aboveground part is mainly used as raw material, and about 10-25% of the flowers are in the flowering stage to The best time is when no more than 10% of the flowers wither; the root can only be excavated as raw material after the aboveground part withers and before the new aboveground part germinates in the next year.

2) Pulverization: After harvesting the aerial parts and roots, they are collected separately for later processing. Pick out the weeds and other sundries from the aboveground part, quickly wash away the sediment and other sundries from the root, and quickly dry it, then immediately crush (the aboveground part) and slice (root) so that the length is about 1.4-5.1cm. The thickness is preferably between 0.5-1cm, and the maximum is no more than 2cm. Too thin is not conducive to filtration. For ease of utilization, slices should be of uniform size; in order to prevent the decomposition of cichoric acid, crushing is best carried out in a cold room at 4-10°C, and the time between harvesting and crushing slices should be minimized as much as possible, and fresh slices Quick storage.

3) Control relative humidity, adjust oxygen, and treat at low temperature for a certain period of time: Freshly crushed and sliced materials can be placed in polyethylene bags, burlap bags, or paper bags. It is best to have several openings of 2-3cm on the bag and leave it open so that the contents are fully exposed to the air. The bag should fit the right amount of slices and not squeeze too hard to prevent rotting or loss of cichoric acid. For example, 10kg slices can be placed in a 10kg polyethylene bag. Then quickly put it into a semi-aerobic freezing environment, keep its relative humidity between 40-85%, preferably 84%, and the temperature between 0-10°C, preferably 3°C. After about 24 hours, the chicory Acid levels started to increase and continued to increase until 96 hours, after which cichoric acid levels showed a downward trend. Therefore, the chicory crushed raw materials should be processed in the next step during this time period.

2. Drying treatment

The processed chicory raw materials after harvesting can be directly processed in the next process, but most of the raw materials need to be temporarily stored for long-term production. In order to prevent the degradation of cichoric acid in plants, it needs to be dried so that the moisture content in the raw material is less than 10%. Microwave vacuum drying, freeze-sublimation drying and blast drying at an appropriate temperature can all achieve the purpose of drying, and will not cause decomposition of cichoric acid. The most suitable temperature for blast drying is 40°C, and the effect of drying for 55 hours under this condition is even better than that of microwave vacuum drying for 47 minutes. If air-dried at 25°C, the loss of cichoric acid is 50%, and at 70°C, the loss of cichoric acid will be greater. In short, as the drying temperature increases over 40°C, the drying time decreases and the loss of cichoric acid gradually increases.

3. Extraction

1) Ethanol extraction method for the aerial parts of chicory: method one: add 40%-70% ethanol or methanol solution (v/v) to the dried chicory material after harvesting as the extraction solvent, and the ratio of solid to liquid is 1:3-1: 20 (G/V) and while stirring, feed nitrogen to prevent degradation of cichoric acid. Place at room temperature for 30 hours to extract cichoric acid. If the ethanol concentration is too high, the degradation degree of cichoric acid will increase; if the ethanol concentration is too low, the extraction will be incomplete and the yield will be reduced. Nitrogen was fed at a rate of 500 L/hour. Method 2: 40%-70% ethanol or methanol solution (v/v) is used as the extraction solvent, ultrasonically extracted for 40 minutes, and the ratio of solid to liquid is 1:3-1:10 (G/V). Separation by centrifugation or pressing to obtain a chicory acid extract, the residue is extracted again, and the extracts are combined.

2) Chicory root water leaching method

Grind the dried chicory root material of freeze treatment carefully, add boiling water, the ratio of material to liquid is 1: 2-1: 20 (G/V), and extract in water for 2-4.5 minutes. This water extraction can increase the yield. Compared with alcohol extraction, the water extraction operation method can increase the yield by 12%, or even reach 17%. Cool to 60°C, keep this temperature for 4 hours, the content of cichoric acid will increase. The juice is squeezed by a mechanical screw press, and the obtained solid residue is repeatedly leached twice, and the cichoric acid extract is combined.

4. Preparation of cichoric acid products:

1) Preparation of Chichoric Acid Product A

The cichoric acid extract obtained from the aerial parts of chicory is filtered through a hollow inorganic fiber microfiltration membrane to remove proteins, tannins, polysaccharides and other large molecular weight interfering components, as well as impurities such as microorganisms, bacteria, and viruses. μm, and then condensed by a rotary thin film evaporator at 40°C, dried at 60°C and a vacuum of 0.08MPa to obtain the finished product of cichoric acid, the humidity of which is less than 8%, preferably less than 5%, the cichoric acid powder has a high content of cichoric acid In 30%, namely cichoric acid product A.

2) Preparation of cichoric acid product B:

① Refining of root chicory acid extract: concentrate the root chicory acid extract to less than 1/2 of the original extract, then add 1-5 times the amount of 95% alcohol (V/V), after adding alcohol, the alcohol content of the solution The content is not less than 60% (V/V), standing for 2-5h, suction filtration or centrifugation to remove polysaccharides and other precipitated substances in the juice, and centrifugation to obtain juice with a higher concentration of cichoric acid. The alcohol can be ethanol, methanol, isopropanol, etc. Preferably, the pore size is 0.05-0.25 μm, and it is best to choose a hollow fiber inorganic ultrafiltration membrane with a pore size of 0.05-0.1 μm to completely remove bacteria, coagulation, water-soluble proteins, colloids, pigments and other insoluble substances to obtain refined extraction of cichoric acid liquid.

②Preparation of cichoric acid product B: the extract is vacuum-concentrated at 40-50°C until the content of solids in the extract is between 20%-35%, preferably between 25%-30%, and then heated to 80% ℃, add maltose or maltodextrin, tricalcium phosphate and other non-hygroscopic carriers to the concentrated solution. Preferably 0.25:1. Then it is dried by means of vacuum drying, spray drying and the like. It is best to use the spray drying method, the inlet temperature is controlled between 149-160°C, and the outlet temperature is controlled between 93-104°C. The obtained product is filtered through a 100-mesh sieve to ensure that at least 40% of it passes through, or at least 100% can pass through a 16-mesh sieve, and finally obtain high-purity cichoric acid powder with a cichoric acid content of more than 20%, that is, cichoric acid products b.

This powder can be made into capsules, tablets or combined with other herbal substances.

5. Preparation of cichoric acid product C:

①Macroporous adsorption resin adsorption separation: the above-mentioned refined cichoric acid extract obtained from the aerial parts or roots of chicory, concentrated to remove alcohol, re-dissolved with distilled water or deionized water, and put the sample on the column at a speed of 10ml/min. The resin is allowed to stand for more than 5 hours for adsorption equilibrium. After washing with water to remove impurities not adsorbed by the resin, then elute with 5%-10% (V/V) methanol-water, preferably 7% (V/V) methanol-water, collect the eluent, 40 Concentrate with a rotary thin film evaporator at ℃, remove methanol, and obtain cichoric acid with better purity. The macroporous adsorption resin adsorption, separation and purification of cichoric acid is easy to operate, simple and convenient, with good reproducibility, less consumption, easy activation and regeneration of the resin, and provides raw materials for the next step of preparing high-purity cichoric acid. Choose HPD-100, D-101A, LSA-30 and other non-polar macroporous adsorption resins for adsorption, and HPD-100 is the first choice.

②C-18 reverse silica gel column chromatography: the better purity chicory acid extract obtained by purifying the macroporous adsorption resin is dissolved in methanol solution, put on a C-18 silica gel (40-60 mesh) column, and use 50% Eluted with methanol solution, using toluene-ethyl acetate-formic acid (5:2:0.7) as developing solvent, developed on silica gel GF254 thin plate, using cichoric acid standard as control, detected and combined to collect cichoric acid eluate, concentrated, removed Methanol and water recrystallization, freeze-sublimation drying to obtain pure cichoric acid, the content of which is more than 90%. Store in low temperature freezer.

The following are preferred embodiments given by the inventors.

Embodiment 1: post-harvest treatment of chicory leaves:

Collect fresh chicory leaves in June, pick out weeds, dead branches and other sundries, quickly crush and cut into 1.5cm segments, the humidity is about 73%, and place 10 kg of crushed leaves in a container with a bulk density of 10 kg within 10 hours. Put it in a polyethylene bag, open it completely, and cut two 2.5cm long incisions on both sides, quickly put it into a cold storage at a temperature of 3°C, keep its relative humidity at 84%, and measure the content of cichoric acid by HPLC after 24 hours of freezing treatment It began to rise and reached the highest value in 96 hours, and its content increased by 1.03% compared with the original. Then the treated chicory leaves were air-dried at 40° C. for 55 hours to obtain dried chicory leaves, which were stored for one year, and the content of chicory acid was determined to be substantially unchanged.

Embodiment 2: Preparation of powdery cichoric acid product A:

Harvest the aerial parts of chicory at the full flowering stage in July, and obtain the raw material that the chicory acid content is increased to 1.7% through the same treatment method as in Example 1. Take 100 grams of this raw material, add 500mL50% ethanol solution, ultrasonic extraction for 40min, and vacuum filtration to obtain Chichoric acid extract and residue were extracted once more, and the extracts were combined. Select a microfiltration membrane with a pore size of 0.25 μm to remove impurities to obtain a refined extract of cichoric acid, concentrate it by rotary thin-film evaporation at 40°C, and dry it in vacuum at 60°C to obtain a powdered cichoric acid product A with a moisture content of 6%. The content of cichoric acid was determined by HPLC was 21%.

Embodiment 3: Preparation of cichoric acid product B:

Harvest chicory root in September, obtain the raw material that chicory acid content increases to 0.9% through the same treatment method as Example 1, get this raw material 100 grams, add 400mL boiling water and extract for 3 minutes, cool to 60 ℃, keep this temperature place 4 hours. The juice is squeezed through a screw press to obtain juice and pomace. Re-extract the residue once, combine the extract, concentrate to 1/2 of the extract, add 3 times the amount of 95% ethanol, precipitate to remove polysaccharide and other precipitates, and then pass through a 0.1μm ultrafiltration membrane to remove other interfering components, and then continue to concentrate until soluble The solids content is 20%. Add carrier tricalcium phosphate 0.315g at 80°C, and then vacuum-dry the obtained powder to pass through a 16-mesh sieve to finally obtain high-purity cichoric acid powder, i.e. cichoric acid product B, whose cichoric acid content is as high as 34%.

Embodiment 4: preparation of cichoric acid product C

Get the cichoric acid refining extract that embodiment 2 or embodiment 3 obtains 500mL, concentrate and remove alcohol, redissolve in deionized water, with the speed of 10ml/min, HPD-100 type macroporous adsorption resin column is put on the sample, and the resin is fully static Set for adsorption equilibrium for 5 hours, elute with 900mL water, remove impurities that are not adsorbed by the resin, elute with 7% (V/V) methanol-water, track and detect with TLC, collect the eluate, and concentrate with a rotary thin film evaporator at 40°C , to obtain the better chicory acid extract of purity, the chicory acid extract is dissolved with a small amount of methanol solution, put on a C-18 silica gel (40-60 mesh) column, elute with 50% methanol solution, TLC tracking detection, and collect chicory The acid eluate was concentrated, methanol was removed, recrystallized with water, and freeze-sublimated dried to obtain pure cichoric acid with a content of 92%. Store in low temperature freezer. For TLC detection, toluene-ethyl acetate-formic acid (5:2:0.7) was used as developing solvent, developed on silica gel GF254 thin plate, and chicory acid standard was used as control.

Claims (3)

1. a kind of technique that utilizes chicory to prepare cichoric acid, is raw material with the root, stem, leaf of chicory, it is characterized in that, this technique comprises the following steps: 1) Post-harvest treatment of chicory a. Chicory harvesting: choose chicory planted for 1-3 years, select the stems and leaves of the aboveground part as raw materials during the full flowering stage, and dig the roots as raw materials after the stems and leaves of the aboveground parts wither; b. Grinding: Immediately pulverize the stems and leaves of the harvested aerial parts in a cold room at 4-10°C; quickly wash away the silt and debris, quickly air-dry and slice them into pieces; c. Control relative humidity, adjust oxygen, and low-temperature treatment: Put the crushed stem and leaf raw materials and sliced root raw materials into a polyethylene bag or burlap bag or paper bag, keep it open, and expose the raw materials to the air completely Among them, there are several 2-3cm openings on the bag, and then the bag is quickly placed in a freezing environment, and its relative humidity is maintained at 40-85%, the temperature is between 0-10°C, and the time is 96 hours; The chicory raw material processed above is directly processed in the next process, or dried by microwave vacuum drying or freeze-sublimation drying, so that the moisture content in the raw material is less than 10%, and it is processed and used later; 2) Extraction step The extraction is divided into the stem and leaf raw material extraction of the aerial part and the chicory root raw material extraction a. Stem and leaf raw material extraction of aerial parts: Add 40% by volume to 70% by volume of ethanol or methanol solution to the dried chicory raw material as the extraction solvent, the ratio of solid to liquid is 1:3g/ml～1:20g/ml, and while stirring, the rate of feeding nitrogen is 500L/hour To prevent the degradation of cichoric acid; place it at room temperature for 30 hours, then extract the cichoric acid to obtain a cichoric acid extract; Or add 40 volume %-70 volume % ethanol or methanol solution to the chicory raw material as an extraction solvent, use ultrasonic extraction for 40 minutes, and the material-liquid ratio is 1:3g/ml～1:10g/ml; centrifuge or squeeze to obtain the chicory acid extract, The slag was extracted again, and the extracts were combined; b. Raw material extraction of chicory root: Grind the dried chicory root material that has been frozen, add boiling water, the ratio of material to liquid is 1:2g/ml～1:20g/ml, extract in water for 2～4.5 minutes, cool to 60°C and place it at this temperature for 4 hours, squeeze the juice through a mechanical screw press, and repeat the leaching of the obtained solid residue twice, and combine the chicory acid extract; 3) Prepare cichoric acid powders with different contents according to different product content requirements from the cichoric acid extract prepared in the above extraction step. 2. method as claimed in claim 1, is characterized in that, the cichoric acid powder that described content is different comprises cichoric acid product A, cichoric acid product B, cichoric acid product C, and its preparation method is respectively: a. prepare cichoric acid product A The cichoric acid extract obtained by extracting the stems and leaves of the aerial part is filtered through a hollow inorganic fiber microfiltration membrane to remove proteins, tannins, polysaccharides and other large molecular weight interference components as well as impurities such as microorganisms, bacteria, viruses, etc., and the microfiltration membrane The pore size is between 0.10μm and 0.25μm, and then concentrated by a rotary thin film evaporator at 40°C, dried at 60°C and a vacuum of 0.08MPa to obtain cichoric acid powder. The content of the cichoric acid powder is higher than 30%, which is a cichoric acid product. A; b. Preparation of Chichoric Acid Product B ①Refinement of chicory acid extract from chicory root raw material: Concentrate the root chicory acid extract to less than 1/2 of the original extract, then add 1-5 times the amount of 95% alcohol, unit: volume %, after adding alcohol, the alcohol content of the solution is not less than 60%, unit: % by volume, stand for 2-5h, suction filtration or centrifugal separation, remove polysaccharides and other precipitated substances in the juice, centrifugal filtration to obtain a juice containing a higher concentration of cichoric acid; select hollow fiber inorganic ultrafiltration with a pore size of 0.05-0.25 μm membrane, completely remove bacteria, solidified and water-soluble proteins, colloids, pigments and other insoluble matter, to obtain a refined extract of cichoric acid; ② Preparation of cichoric acid product B: Concentrate the refined cichoric acid extract under vacuum at 40-50°C until the solid content in the extract is between 20% and 35%, then heat to 80°C, add maltose or maltodextrin, triphosphate Calcium non-hygroscopic carrier, the ratio of the added amount to the soluble solid is 0.2:1~1.2:1, and then dried by vacuum drying and spray drying, and the product obtained after drying is filtered through a 100-mesh sieve to ensure that it has at least 40% pass; or 100% pass through a 16-mesh sieve to finally obtain a cichoric acid powder with a cichoric acid content of more than 20%, which is the cichoric acid product B; c. Preparation of Chichoric Acid Product C ①Macroporous adsorption resin adsorption separation: Concentrate the above-mentioned cichoric acid refined extract obtained from the aerial parts or roots of chicory to remove alcohol, redissolve it with distilled water or deionized water, and put the sample on a macroporous adsorption resin column at a speed of 10ml/min, and let the resin fully stand Adsorption balance for more than 5 hours, wash with water to remove impurities that are not adsorbed by the resin, and then elute with 5%-10% methanol-water, unit: volume%, collect the eluate, concentrate with a rotary thin film evaporator at 40°C, and remove methanol. to obtain cichoric acid; ②C-18 reverse silica gel column chromatography: The cichoric acid extract obtained from the purification of the macroporous adsorption resin is dissolved in methanol solution, put on a C-18 silica gel column, and eluted with 50% methanol solution, and developed with toluene:ethyl acetate:formic acid equal to 5:2:0.7 reagent, silica gel GF ₂₅₄ thin plate development, with cichoric acid standard product as a control, detection and combined collection of cichoric acid eluate, concentration, removal of methanol, water recrystallization, freeze-sublimation drying, to obtain pure cichoric acid with a content of more than 90%. That is the cichoric acid product c. 3. The method according to claim 2, wherein the inlet temperature of the cichoric acid product B is controlled between 149°C and 160°C, and the outlet temperature is controlled between 93°C and 104°C when the spray drying method is used to prepare the cichoric acid product B.

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