CN111053044A

CN111053044A - Behavioural device for detecting mating willingness of rodent

Info

Publication number: CN111053044A
Application number: CN201911277964.XA
Authority: CN
Inventors: 李蕾; 杨青宁; 王立平; 申培磊; 林子逸; 李栩琳; 李子杰; 纪树钦
Original assignee: Shenzhen Institute of Advanced Technology of CAS
Current assignee: Shenzhen Institute of Advanced Technology of CAS
Priority date: 2019-12-12
Filing date: 2019-12-12
Publication date: 2020-04-24

Abstract

The invention discloses a behavioural device for detecting mating willingness of rodents, which comprises a box body with an opening at the top end and a partition plate, wherein the box body is provided with a clamping groove, the partition plate is detachably connected with the box body through the clamping groove, the partition plate is provided with a female rodent channel, and the partition plate is a transparent perforated partition plate. The invention can more accurately reflect the mating willingness of the animals. The method comprises the following specific steps: 1) the animal activity space simulates the size of a feeding environment, and the stability of animal behaviors is ensured. 2) The baffle is transparent ventilates, guarantees that the animal can obtain sufficient vision and smell information, does benefit to the output and the observation of action. 3) The baffle is removable, and the baffle of optional equidimension passageway adapts to the not animal of equidimension, guarantees the stable output of animal action. 4) The device is simple in design and easy to clean. 5) The invention can determine the mating willingness of the female rodent and eliminate the possibility of forced mating, thereby laying a foundation for accurate behavior analysis.

Description

Behavioural device for detecting mating willingness of rodent

Technical Field

The invention belongs to a rodent ethology device, and particularly relates to a ethology device for detecting mating willingness of rodents.

Background

With the rapid development of economic society, environmental deterioration and improvement of the proportion of late marriage and late rearing, a great number of reproductive health problems continuously appear. Relevant investigation reports show that the infertility rate of the population of child bearing age in China is continuously increased. The modern society is sterile and the related reproductive diseases and disorders are high, so that the research on the reproductive diseases has very important significance.

The use of rodents as experimental animals is a practical approach for ethical reasons. Rodents such as mice have the following characteristics compared with other animals 1. small size, mild temperament, easy feeding management; 2. the breeding cycle is short; 3. the feeding cost is low; 4. belongs to mammals and has higher gene similarity with human; 5. as experimental animals have been used for a long time, different strains (genetic backgrounds) and disease research models exist, and corresponding experimental operation and analysis processes are mature. Rodents are therefore a widely used model animal.

The research on the reproduction behavior of the rodent is of great significance for analyzing reproduction-related diseases. By analyzing the mating behavior of the rodent, the mating willingness of the animal under different conditions, specific mating behavior and the like can be further researched. The corresponding ethological research has important reference value for the research of human reproduction related diseases. Therefore, the research device for the corresponding behavior of the rodent is of great significance.

Disclosure of Invention

The invention aims to provide a rodent mating intention behavior detection device with stable behavioral output, which achieves the purpose that the mating intention of rodents (mainly female) needs to be detected in a behavioral experiment and more accurately reflects the mating intention of the rodents. Through rodent mating wish action detection device, provide safe good experimental environment for the rodent for stable behavioural data can be gathered.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows: the utility model provides a behavioural device that rodent mating wish detected, includes open-ended box and baffle in the top, is equipped with the draw-in groove on the box, and the baffle passes through the draw-in groove and can dismantle with the box and be connected, is equipped with female rodent passageway on the baffle, and the baffle is the transparent tape hole baffle.

Furthermore, the clamping groove is an inner clamping groove or an outer clamping groove.

Further, the partition includes a plurality of partitions provided with female rodent passageways of different sizes.

Further, the body weight and chest circumference of the rodents are measured before the formal experiment, and the partition plates provided with the passages for the female rodents with different sizes are selected according to the rodents with different body types.

Further, a female rodent passageway is provided in the bottom of the partition.

Further, the female rodent passageway is a semicircular aperture.

Further, the box body 1 and the partition plate 2 are made of acrylic plates, and the acrylic plates used by the box body are opaque.

Furthermore, the space size behind the baffle partition box body simulates the size of a rodent feeding environment cage, and the animal is ensured to have enough activity space.

The existing device for detecting the mating behavior of the rodent cannot completely and accurately reflect the mating desire of the rodent. Compared with the prior art, the invention has the beneficial effects that:

the invention can reflect the mating willingness of the animals more accurately. The method comprises the following specific steps: 1) the animal activity space simulates the size of a feeding environment, and the stability of animal behaviors is ensured. 2) The baffle is transparent ventilates, guarantees that the animal can acquire sufficient vision and smell information, does benefit to the output and the observation of action, has guaranteed the stability of animal action. 3) The baffle is removable, and the baffle of optional equidimension passageway adapts to the not animal of equidimension, guarantees the stable output of animal action. 4) The device design is succinct, can make things convenient for, swiftly carry out experimental operation, easily cleanness. 5) The invention prevents the situation that the female rodent is forced to mate under a plurality of conditions because the male rodent is stronger, can determine the mating willingness of the female rodent, and eliminates the possibility of forced mating, thereby laying a foundation for accurate behavior analysis.

Drawings

FIG. 1 is a schematic diagram of the configuration of a behavioral apparatus for testing mating willingness of rodents according to the invention;

FIG. 2 is a schematic structural view of the card slot being an inner card slot;

fig. 3 is a schematic structural view of the card slot being an external card slot.

Description of reference numerals:

1. the box, 2, baffle, 3, the draw-in groove, 4, female rodent passageway.

Detailed Description

An embodiment of the present invention will be described in detail below with reference to the accompanying drawings, but it should be understood that the scope of the present invention is not limited to the embodiment.

The utility model provides a behavioural device that rodent mating wish detected, as shown in fig. 1-3, includes open-ended box 1 and baffle 2 in the top, is equipped with draw-in groove 3 on the box 1, and baffle 2 passes through draw-in groove 3 and can dismantle with box 1 and be connected, is equipped with female rodent passageway 4 on the baffle 2, and baffle 2 is the transparent foraminiferous baffle. The box body 1 is used as the experimental environment of the experimental animal. The transparent partition plate 2 with the holes is adopted, so that the rodent can acquire the visual and olfactory information of the other space. The rodent is separated into two spaces, and the two spaces are connected through a channel, so that the mating behavior of the female rat is ensured to be carried out at will.

Further, the card slot 3 is an inner card slot or an outer card slot.

Further, the partition 2 includes a plurality of partitions 2 provided with female rodent passageways 4 of different sizes. The partition plates 2 with the female rodent channels 4 are designed into an alternative mode, and the partition plates 2 with the female rodent channels 4 can be selected according to the body sizes of male rodents and female rodents which are actually detected in behavior, so that the male rodents can only freely move in one side space, and the female rodents can freely pass through the channels to enter the moving range of the male rodents besides freely moving in one side space.

Further, the body weight and chest circumference of the rodents are measured before the official experiment, and the partition plates 2 provided with the female rodent passages 4 of different sizes are selected according to the rodents of different body types.

Further, a female rodent passageway 4 is provided at the bottom of the partition 2.

Further, the female rodent passageway 4 is a semi-circular aperture.

Furthermore, the box body 1 and the partition plate 2 are made of acrylic plates, and the acrylic plates used by the box body are opaque. The acrylic plate is light in weight, low in price, non-toxic, easy to form, good in weather resistance, acid and alkali resistance, high in toughness and not easy to damage; the repairing performance is strong, and the like, and the cleaning after the experiment only needs to be carried out by using 20% alcohol and wiping the paper with 20% alcohol.

Furthermore, the space size behind the partition plate 2 separating the box body 1 simulates the size of a rodent feeding environment cage, and the sufficient activity space of the animals is ensured.

Taking a mouse as an example, the surface of the box body 1 except the top surface is left empty, the other five surfaces are opaque square acrylic plates with the thickness of 0.5cm, and the height of the box body 1 and the partition plate 2 is 50 cm. The left side of the clamping groove 3 is taken as the left side and the right side, and the other two sides are taken as the front side and the back side. Wherein the bottom surface is 40cm × 30cm, the left and right side surfaces are 40cm × 50cm, and the front and back side surfaces are 30cm × 50 cm. The middle transparent porous partition 2 has the size of 40cm multiplied by 50cm and the thickness of 0.5 cm. The radius of the semicircular hole of the female rodent passageway 4 is 1cm, 1.2cm, 1.4cm and 1.6cm respectively. Through the selection of the size, the experimental animal feeding environment can be simulated on the one hand, and meanwhile, the sufficient activity space of the experimental animal feeding environment in the box body 1 is ensured, so that the influence of strange environment on animal behaviors is reduced.

Because of the difference in the body types of the mice, before the mice are used formally, the weight of the mice and the chest circumference of the mice should be measured, and the replaceable partition plate 2 with the corresponding aperture should be selected. In the copulation willingness behavior detection, male and female mice are respectively placed at two sides of the box body, and if the female mice have copulation willingness, the female mice can go to the area where the male mice are located through the bottom channel. If the female mouse has no copulation intention, the female mouse can not go to the area where the male mouse is located, and the male mouse can not go to the area where the female mouse is located through a small hole to force the female mouse to copulate.

The invention is designed to be suitable for rodents represented by mice, but the device can be suitable for other experimental animals to carry out experimental operation after the sizes of all parts of the device are modified. In addition, the device is suitable for experiments of a pair of (two) animals, and a plurality of devices can be placed under the condition of permission of laboratory space, so that the experimental efficiency is improved.

The above description is only a specific embodiment of the present invention, and not all embodiments, and any equivalent modifications of the technical solutions of the present invention, which are made by those skilled in the art through reading the present specification, are covered by the claims of the present invention.

Claims

1. The utility model provides a behavioural device that rodent mating wish detected, a serial communication port, including top end open-ended box (1) and baffle (2), be equipped with draw-in groove (3) on box (1), baffle (2) can be dismantled with box (1) through draw-in groove (3) and be connected, be equipped with female rodent passageway (4) on baffle (2), baffle (2) are transparent foraminiferous baffle.

2. A behavioural device for rodent mating willingness test according to claim 1, characterised in that the card slot (3) is an internal or external card slot.

3. A behavioural device for rodent mating willingness detection according to claim 1, characterised in that the partition (2) comprises a plurality of partitions (2) provided with female rodent channels (4) of different sizes.

4. Behavioural device for rodent mating willingness detection according to claim 3, characterised in that the weight and chest circumference of rodents are measured before the official experiment, and that the spacer (2) is selected with female rodent channels (4) of different sizes according to the rodent size.

5. A behavioural device for rodent mating willingness test according to claim 1, characterised in that the female rodent passageway (4) is provided at the bottom of the partition (2).

6. A behavioural device for testing the mating willingness of rodents according to claim 1, wherein said female rodent pathway (4) is a semi-circular hole.

7. A behavioural device for detecting the mating willingness of rodents according to claim 1, wherein the box body (1) and the partition plate (2) are both made of acrylic plates, and the acrylic plates used by the box body are opaque.

8. A behavioural device for testing the mating willingness of rodents according to any one of claims 1 to 7, wherein the space after the partition boards (2) divide the box body (1) simulates the size of a cage of a rodent breeding environment, and the sufficient activity space of the rodents is ensured.