CN111035715A - Application of traditional Chinese medicine composition in preparation of medicine for improving gastrointestinal function and regulating intestinal flora - Google Patents

Abstract

The invention discloses application of a traditional Chinese medicine composition in preparing a medicine for improving gastrointestinal function and regulating intestinal flora, wherein the traditional Chinese medicine composition comprises the following components: astragalus root, white atractylodes rhizome, tangerine peel, lilyturf root, baikal skullcap root, haw and radish seed. The invention can regulate gastrointestinal hormone and neuropeptide related to appetite, improve the micro-ecology of intestinal flora, treat and prevent digestive system diseases which are caused by diet and are mainly characterized by dyspepsia, improve the general physical sign state of infants, relieve symptoms such as anorexia and abnormal defecation, improve the activity of enzymes in the intestinal tract, promote digestive absorption and further improve the nutritional level of organisms.

Description

Application of traditional Chinese medicine composition in preparation of medicine for improving gastrointestinal function and regulating intestinal flora

Technical Field

The invention relates to a new application of a traditional Chinese medicine composition, in particular to an application of a traditional Chinese medicine composition in preparing a medicine for improving gastrointestinal function and regulating intestinal flora.

Background

According to the annual book of national sanitation and family planning statistics in 2015, the prevalence rate of digestive system diseases is ranked the fourth of the prevalence rate of chronic diseases in China and is in the front. According to incomplete statistics, the total incidence rate of digestive system diseases accounts for 30% of the population and the number of deaths caused by the digestive system diseases accounts for 14% of the total deaths on the global scale. Because the digestive system organs of the infants are not developed completely, the digestive and absorptive functions are relatively weak, and besides, improper feeding of parents causes irregular diet, hunger and satiety, partial AIDS and the like of the children, the incidence of digestive system diseases in the infants is high. Digestive system diseases mostly show symptoms such as poor appetite, regurgitation, abdominal pain, dyspepsia, abnormal defecation and the like, can seriously affect the digestion, absorption and metabolism of nutrient substances, and further affect the healthy growth of infants and the quality of family life. The western medicine for treating digestive system diseases is usually applied to the disease, and chemical drugs related to the treatment of the digestive system diseases are sold in the market, and comprise antacids, acid suppressants (H2 receptor antagonist and proton pump inhibitor), selective anticholinergic agents, gastric mucosa protective agents, prostaglandins and derivatives, gastrointestinal spasmolytic agents, digestion-promoting agents, gastrointestinal motility-promoting and antiemetic agents, laxative and antidiarrheal agents, microecological drugs and other ulcer treatment drugs. These drugs often have many and large side effects, and many chemical drugs have been warned and even suggested to be restricted for use many times abroad. The traditional Chinese medicine considers that the spleen is mainly transported and transformed, and dyspepsia is always caused by the spleen, so that traditional Chinese medicines for tonifying spleen, transporting spleen, eliminating dampness, promoting diuresis and the like are often adopted to treat diseases related to digestion. These traditional Chinese medicines are complex in ingredients, but have diverse and integrated efficacies. Traditional Chinese medicine has unique action mechanism for treating digestive system diseases, and often has multiple effects at the same time, so that the traditional Chinese medicine is selected and favored by more and more families of patients.

The intestinal flora is a collective term for a variety of microorganisms that colonize the gastrointestinal tract of healthy humans and animals, and that not only assume digestive and nutritional functions, but also play a role in both immunomodulation and neuromodulation. The intestinal flora is closely related to the functions of the digestive system, and the imbalance of the intestinal flora can cause a series of digestive tract diseases. At present, the methods for regulating the intestinal flora mainly comprise antibiotics, prebiotics (polysaccharides), probiotics, intestinal flora transplantation and the like. The broad sterilization spectrum and drug resistance of the antibiotics make the antibiotics unsuitable for long-term application; the prebiotic preparation of pure oligosaccharide has poor effect and does not have the inhibiting effect on conditional pathogenic bacteria and pathogenic bacteria; probiotic preparations tend to be ineffective due to harsh storage conditions and loss through the stomach. The transplantation of intestinal flora is further at risk of infection and death of multiple drug-resistant bacteria. More and more researches show that the traditional Chinese medicine has natural advantages in the aspect of regulating the intestinal flora and the fine conditioning for a long time.

The astragalus and the white atractylodes rhizome in the traditional Chinese medicines tonify spleen and qi, strengthen exterior and stop sweating; the dried orange peel is used for regulating qi and activating spleen; radish seed and hawthorn fruit can promote digestion; the scutellaria baicalensis and the ophiopogon root have the effects of relieving fever and promoting the secretion of saliva; the compatibility of the medicines achieves the curative effect of strengthening spleen and promoting digestion. Modern pharmacological studies show that the medicines have the effects of resisting inflammation, resisting bacteria, relieving pain, regulating immunity, resisting fatigue, resisting endothelial cell injury, eliminating phlegm, relieving spasm and the like, but the research on whether the medicines influence the neuroendocrine system and the microecology of intestinal flora and on the specific action mechanism of strengthening spleen and helping digestion is less.

The traditional Chinese medicines for treating the digestive system diseases are numerous, but the research on the specific mechanism of the multiple effects of the traditional Chinese medicines is few, so that the mechanism of improving the digestion and treating the digestive system dysfunction of the medicines is researched according to the physiological characteristics of the children and the combination of the traditional Chinese medicine and the western medicine of the digestive system diseases, and the development of the traditional Chinese medicine composition which has comprehensive functions and can regulate the functions of the digestive system has important social significance and economic value.

Disclosure of Invention

In order to solve the technical problems, the invention aims to provide an application of a traditional Chinese medicine composition in preparing medicines for improving gastrointestinal functions and regulating intestinal flora, wherein the traditional Chinese medicine composition can regulate gastrointestinal hormone and appetite-related neuropeptide, improve the micro-ecology of the intestinal flora, and treat and prevent digestive system diseases which are caused by diet and mainly characterized by dyspepsia.

In order to achieve the purpose, the invention provides the following technical scheme:

the application of a traditional Chinese medicine composition in preparing a medicine for improving gastrointestinal function and regulating intestinal flora comprises the following components: astragalus root, white atractylodes rhizome, tangerine peel, lilyturf root, baikal skullcap root, haw and radish seed.

The invention also provides application of the traditional Chinese medicine composition in preparing a medicine for regulating gastrointestinal hormone related to digestion and neuropeptide secretion related to appetite or a medicine for regulating the microecology of intestinal flora and improving the structure of the intestinal flora.

Preferably, the gastrointestinal hormones include motilin, gastrin and somatostatin, and the neuropeptides include β -endorphin and cholecystokinin octapeptide.

As a preferred technical scheme, the dosage forms of the medicines comprise tablets, granules, tea bags, oral liquids, capsules and the like.

As a preferable scheme, the traditional Chinese medicine composition further comprises refined honey, sorbic acid or potassium sorbate, and the weight parts of the components are as follows: 20-500 parts of astragalus membranaceus, 10-250 parts of fried bighead atractylodes rhizome, 10-250 parts of dried orange peel, 20-500 parts of radix ophiopogonis, 10-250 parts of scutellaria baicalensis, 10-250 parts of fried hawthorn, 10-250 parts of fried radish seed, 80-2500 parts of refined honey and 0.1-5 parts of sorbic acid or potassium sorbate.

Preferably, the weight parts of the components are as follows: 50-300 parts of astragalus membranaceus, 25-150 parts of fried bighead atractylodes rhizome, 25-150 parts of dried orange peel, 50-300 parts of radix ophiopogonis, 25-150 parts of scutellaria baicalensis, 25-150 parts of fried hawthorn, 25-150 parts of fried radish seed, 200-1500 parts of refined honey and 0.3-3 parts of sorbic acid or potassium sorbate.

Further preferably, the weight parts of the components are as follows: 66.7 parts of astragalus, 33.3-33.4 parts of fried bighead atractylodes rhizome, 33.3-33.4 parts of dried orange peel, 66.7 parts of dwarf lilyturf tuber, 33.3-33.4 parts of radix scutellariae, 33.3-33.4 parts of fried hawthorn, 33.3-33.4 parts of fried radish seed, 300 parts of refined honey, 0.5 part of sorbic acid or 0.67 part of potassium sorbate.

As a preferred technical scheme, the astragalus, the bighead atractylodes rhizome, the dried orange peel, the dwarf lilyturf tuber, the radix scutellariae, the hawthorn and the radish seed are respectively replaced by extracts thereof;

the extract is water extract or alcohol extract with the volume concentration of 50-90%.

As a preferred technical scheme, the refined honey is used as an excipient, a thickening agent, an auxiliary material and/or a flavoring agent.

As a preferable technical scheme, when the dosage form of the traditional Chinese medicine composition is liquid, the preparation method comprises the following steps:

(1) weighing radix astragali, fried bighead atractylodes rhizome, dried orange peel, dwarf lilyturf tuber, radix scutellariae, fried hawthorn and fried radish seed according to the parts by weight, adding water, decocting for 2-3 times, each time for 2-3 hours, and combining the decoctions;

(2) concentrating the combined decoction to obtain clear paste with the relative density of 1.01-1.05, refrigerating for 24-48 hours, and filtering to obtain filtrate;

(3) adding refined honey, sorbic acid and/or potassium sorbate into the filtrate in parts by weight, adding water to the total volume of 1-800L, shaking up, standing for 24-48 hours, taking the supernatant, and filtering to obtain the product.

As a preferable technical scheme, in the step (1), decoction liquid is carried out in an extraction tank, when the decoction is carried out, the vapor pressure of the extraction tank is less than or equal to 0.15Mpa, the temperature of the extraction liquid is 95-100 ℃, and a liquid circulation pump is started every 20 minutes, and the extraction liquid circulates for 5 minutes up and down;

in the step (2), concentration is carried out in a concentration heater, the vapor pressure of the concentration heater is 0.05-0.15 Mpa, the concentration vacuum degree is 0.06-0.08 Mpa, and paste is collected in a clean area.

Compared with the prior art, the invention has the following advantages:

1. can regulate the secretion of gastrointestinal hormone related to digestion and neuropeptide related to appetite, and is also one of the pharmacodynamic mechanisms of promoting digestion and improving appetite;

2. can regulate the micro-ecology of the intestinal flora and improve the structure of the intestinal flora, and is an important basis for exerting the pleiotropic effect on the aspect of treating digestive system diseases;

3. can improve the general physical sign state of infants, and relieve symptoms such as anorexia and abnormal defecation;

4. can improve the activity of enzymes in intestinal tracts, promote digestion and absorption and further improve the nutritional level of organisms.

Detailed Description

The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto. The reagents, equipment and methods adopted by the invention are all reagents, equipment and methods which are conventionally and commercially available in the technical field and are conventionally used in the technical field.

Example 1

Preparing oral liquid:

(1) taking 66.7g of astragalus root, 33.4g of fried largehead atractylodes rhizome, 33.4g of dried orange peel, 66.7g of dwarf lilyturf tuber, 33.4g of baical skullcap root, 33.4g of fried hawthorn fruit and 33.4g of fried radish seed; decocting in water twice, each time for 2 hours, filtering, combining filtrates, and concentrating to obtain fluid extract with relative density of 1.01-1.05 (measured at 60 deg.C);

(2) refrigerating for 48 hours, and filtering to obtain filtrate;

(3) adding refined honey 300g and potassium sorbate 0.67g (adding appropriate amount of water for hot dissolution), adding water to 1000ml, shaking, standing for 48 hr, collecting supernatant, and filtering;

(4) encapsulating, and sterilizing.

Example 2

Preparing oral liquid:

(1) taking 66.7g of astragalus root, 33.3g of fried largehead atractylodes rhizome, 33.3g of dried orange peel, 66.7g of dwarf lilyturf tuber, 33.3g of baical skullcap root, 33.3g of fried hawthorn fruit and 33.3g of fried radish seed; decocting in water twice, each time for 2 hours, filtering, combining filtrates, and concentrating to obtain fluid extract with relative density of 1.01-1.05 (measured at 60 deg.C);

(2) refrigerating for 48 hours, and filtering to obtain filtrate;

(3) adding refined honey 300g and sorbic acid 0.5g into the filtrate (adding appropriate amount of water for hot dissolution), adding water to 1000ml, shaking, standing for 48 hr, collecting supernatant, and filtering;

(4) encapsulating, and sterilizing.

Example 3

Preparing oral liquid:

(1) 53.36kg of astragalus root, 26.64kg of fried largehead atractylodes rhizome, 26.64kg of dried orange peel, 53.36kg of dwarf lilyturf tuber, 26.64kg of baical skullcap root, 26.64kg of fried hawthorn fruit and 26.64kg of fried radish seed are taken; adding water into the seven raw materials, extracting for three times, namely 3 hours for the first time and 2 hours for the second and third times respectively, mixing decoctions, filtering, and concentrating the filtrate into clear paste with the relative density of 1.01-1.05 (measured at 60 ℃);

(2) refrigerating for 48 hours, and filtering to obtain filtrate;

(3) adding 240kg of refined honey and 0.4kg of sorbic acid into the filtrate obtained in the step (2) (adding a proper amount of water for hot dissolution), adjusting the total amount to 800000ml by using water, uniformly stirring, standing for 48 hours, taking supernatant, and filtering;

(4) encapsulating, and sterilizing.

Test example 1

The influence of the oral liquid obtained in example 3 on the physical state of experimental rats with anorexia due to spleen deficiency and the influence on food intake and body weight are explored

Experiment design: by utilizing the reserpine and the special high-fat high-protein feed to replicate an experimental spleen deficiency anorexia rat model, the spleen deficiency experimental rat shows observable signs of food intake reduction, disordered and loose hair, preference for clustering, narrow-eyed listlessness, abnormal feces and the like, and can well represent the effect of spleen deficiency moulding and the drug effect of the drug on improving spleen deficiency. And decreased food intake and slow or arrested weight gain are other characteristics exhibited by spleen deficiency animal models. Therefore, these signs are often used in the study of spleen deficiency in TCM.

The purpose is as follows: the effect of the traditional Chinese medicine composition on improving the physical sign state of rats with spleen deficiency and anorexia and the influence on the food intake and the body weight of the rats with spleen deficiency and anorexia are researched.

Sample preparation: the oral liquid prepared by the method provided in example 3 was used for the study. The feed also comprises a special high-fat high-protein feed (the formula refers to a Wang institute of teaching and education etiology simulation method, fish meal, milk powder, corn flour, soybean flour, white sugar, whole egg powder and lard oil are 1: 1: 2: 1: 0.36: 2, quality qualification No.44200300017316, and the feed is processed by the Guangdong province medical experimental animal center).

The experimental method comprises the following steps: healthy male SPF young rats were divided into 18 groups at random. Group I was blank, group II was model, and group III was dosing. Injecting reserpine 0.2mg/kg/d into abdominal cavity of rats of other groups except group I, and feeding with special high fat and high protein feed for 14 days after spleen deficiency and modeling; after the molding is finished, the oral liquid prepared in group III intragastric administration example 3 is administered in an amount which is 4 times of the amount of human body. At the same time, groups I and II were given equal volume of distilled water for 14 days. Throughout the animal experiment, the general signs of the rats and food intake and body weight were recorded. The scoring criteria are shown in table 1, and the results are shown in tables 2, 3 and 4.

TABLE 1 evaluation chart of rat sign state

TABLE 2 results of rat sign state improvement experiment

Note: compared with blank group, # p <0.05, # p <0.01

From the experimental results shown in table 2, the oral liquid prepared in example 3 can significantly improve the physical sign status of rats with spleen deficiency.

TABLE 3 Change in food intake (g) in rats

As can be seen from the data in Table 3, the oral liquid prepared in example 3 can increase the food intake of the rats with spleen deficiency.

TABLE 4 variation of rat body weight (g)

Note: compared with blank group, # p <0.05, # p <0.01

As can be seen from the data in Table 4, the oral liquid prepared in example 3 is effective in promoting the normal weight gain of rats to a normal level.

Test example 2

The effect of the oral liquid obtained in example 3 on the D-xylose, salivary amylase and total protein content in rat serum was investigated

The purpose is as follows: in order to further prove the influence of the traditional Chinese medicine composition on digestion, absorption and nutrient metabolism, the oral liquid prepared by the method provided in the example 3 is taken as a research object, and the influence on the content of salivary amylase, D-xylose and total protein in rat serum is researched.

The method comprises the following steps: healthy male SPF young rats 24 were randomly divided into 4 groups. Group I was blank, group II was model, group III was positive control, and group IV was dosing. Rats in each group except group I were injected with reserpine 0.2mg/kg/d intraperitoneally and fed with a special high-fat high-protein diet, and after 14 days, group III and group IV were gavaged with domperidone suspension and the oral liquid prepared in example 3, and the administration amounts were 2 times and 4 times of the human dose, respectively. Meanwhile, the groups I and II are given equal volume of distilled water, the rats are gavaged with equal volume of D-xylose solution after 14 days of administration, chloral hydrate anesthesia is performed after 1 hour, abdominal aorta is subjected to blood sampling, and the content of salivary amylase, D-xylose and total protein in serum is detected by a kit detection method. The results are shown in Table 5. As can be seen from Table 5, the oral liquid can significantly increase the levels of serum D-xylose, amylase and total protein, which indicates that the Chinese medicinal composition can effectively promote the absorption of small intestine, improve the substance metabolic enzyme activity and the nutritional state of the body.

TABLE 5D-xylose, salivary amylase and Total protein content in rat serum

Note: compared with blank group, # p <0.05, # p < 0.01; p <0.05, p <0.01 compared to model group.

Test example 3

The influence of the oral liquid obtained in example 3 on appetite-related neuropeptides β -endorphin and cholecystokinin 8 in rat serum was investigated

The purpose is as follows: to further prove the effect of the present Chinese medicinal composition on the improvement of food intake and appetite regulation, the oral liquid prepared by the method provided in example 3 was used as a sample to study the effect of the present Chinese medicinal composition on the adjuvant therapy of anorexia caused by dyspepsia in rats.

The method comprises the steps of adopting blood of a rat tested in test example 2, detecting the contents of β -endorphin and cholecystokinin 8(CCK-8) in the serum by an enzyme-linked immunosorbent assay, wherein the test result is shown in Table 6.

TABLE 6 Effect on β -endorphin, cholecystokinin 8 in rat serum

Note: compared with blank group, # p <0.05, # p < 0.01; p <0.05, p <0.01 compared to model group.

Test example 4

The influence of the oral liquid obtained in example 3 on rat serum motilin, gastrin and somatostatin was investigated

The purpose is as follows: in order to further prove the influence of the traditional Chinese medicine composition on the gastrointestinal motility function, the oral liquid prepared by the method provided in example 3 is used as a sample to research the influence of the traditional Chinese medicine composition on the secretion of the rat gastrointestinal hormone.

The method comprises the following steps: the blood of the rat tested in test example 2 was used to detect the content of motilin, gastrin and somatostatin in the serum by enzyme-linked immunosorbent assay, and the results are shown in Table 7. The results show that the oral liquid prepared in the example 3 can increase the content of motilin and gastrin to a certain degree and reduce the content of somatostatin, which shows that the traditional Chinese medicine composition can regulate the level of gastrointestinal hormone, further influence the gastrointestinal motility function and promote food digestion.

TABLE 7 Effect on motilin, gastrin, somatostatin in rat serum

Note: compared with blank group, # p <0.05, # p < 0.01; p <0.05, p <0.01 compared to model group.

Test example 5

The influence of the oral liquid obtained in example 3 on the intestinal flora was investigated

The purpose is as follows: in order to further prove the regulation effect of the traditional Chinese medicine composition on the intestinal flora, the oral liquid prepared by the method provided in the embodiment 3 is used as a sample to research the influence of the traditional Chinese medicine composition on the intestinal flora of experimental rats.

The method comprises the following steps: test example 1 the contents of the colon segment of the experimental rat were collected and sequenced by 16S rDNA sequencing. The sequencing procedure was as follows: extracting total DNA of bacteria, and detecting extraction results according to the ratio of 260nm/280nm and 260nm/230 nm. The V3-V4 region of the bacterial 16 srrrna gene was amplified using a primer pair (forward primer: 5'-ACTCCTACGGGAGGCAGCA-3'; reverse primer: 5'-GGACTACHVGGGTWTCTAAT-3') in combination with the adapter sequence and barcode sequence. A total of 50. mu.l of PCR amplification system was composed of 10. mu.l buffer, 0.2. mu. l Q5 high fidelity DNA polymerase, 10. mu.l GC enhancer, 1. mu.l dNTP, 10. mu.M each of forward and reverse primers, and 60ng of genomic DNA. Thermocycling conditions included an initial denaturation at 95 ℃ for 5 minutes, followed by 15 cycles comprising 95 ℃ (1min) to 50 ℃ (1min) to 72 ℃ (1min), and a final extension at 72 ℃ for 7 minutes. The PCR product was then purified by VAHTSTM DNA clean beads. Then 2 XPisuion-HF-M M20. mu.l, 8. mu.l double distilled water, 10. mu.M each of forward and reverse primers and 10. mu.l of the PCR product obtained in the first step were mixed and 40. mu.l of the mixed system was used for the second round of PCR. The thermal cycling conditions were as follows: initial denaturation at 98 ℃ for 30s, followed by 10 cycles comprising 98 ℃ (10s) -65 ℃ (30s) -72 ℃ (30s), final extension at 72 ℃ for 5min, and final extension with Quant-iT^TMThe dsDNA-HS reagents aggregate and quantify the product. Finally, high-throughput sequence analysis of bacterial 16S rDNA genes was performed via Illumina Hiseq 2500 platform of beijing bemaimaiche biotechnology limited. The sequencing results are shown in tables 8, 9 and 10.

TABLE 8 enrichment and diversity of intestinal flora

As can be seen from table 8, the oral liquid prepared in example 3 can increase the OUT number, the Ace index, the Chaos index and the Shannon index of the intestinal flora, and decrease the Simpson index, and further proves that the traditional Chinese medicine composition can increase the diversity and the abundance of the intestinal flora and has an intestinal flora regulating effect.

TABLE 9 phylum distribution of intestinal flora in different groups

As can be seen from Table 9, the Chinese medicinal composition can increase Firmicutes, Saccharomyces, Actinobacterium and Cyanobacterium to a certain extent, and reduce the proportion of bacteria, Proteobacteria, Tenericutes and Deferribacteria, so that the proportion of the bacteria is closer to the proportion of the normal group of mouse intestinal bacteria on the phylum classification level.

TABLE 10 analysis of different groups of different genera of bacteria

Note: compared with the blank group, # p <0.05, # p <0.01, # p < 0.001; p <0.05, p <0.01, p <0.001 compared to the model group.

At the taxonomic genus level, 19 genera with significant differences between the blank group and the model group were found. Wherein, the traditional Chinese medicine composition can obviously reduce the relative abundance of Lachnospiraceae _ NK4A136_ group, Prevotellaceae _ NK3B31_ group, Ruminicystridium _6, Prevotellaceae _ UCG-001, Clostridiales _ vadinBB60_ group, Mycoplasma and Tyzzerella, and can increase the relative abundance of Erysipelotrichaceae, Christenseella _ R-7_ group, Escherichia-Shigella, Bifidobacterium, Candidatus _ Saccharionis, Marvinmilbrunia, Fary _ XIII _ 3011_ group and Senellimasilia, further proves that the traditional Chinese medicine composition has the function of regulating intestinal flora.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and their concepts should be considered to be equivalent or modified within the technical scope of the present invention.

Claims (10)

1. The application of a traditional Chinese medicine composition in preparing a medicine for improving gastrointestinal function and regulating intestinal flora comprises the following components: astragalus root, white atractylodes rhizome, tangerine peel, lilyturf root, baikal skullcap root, haw and radish seed.

2. The application of a traditional Chinese medicine composition in preparing a medicine for regulating gastrointestinal hormone related to digestion and neuropeptide secretion related to appetite or regulating the microecology of intestinal flora and improving the structure of the intestinal flora, wherein the traditional Chinese medicine composition comprises the following components: astragalus root, white atractylodes rhizome, tangerine peel, lilyturf root, baikal skullcap root, haw and radish seed.

3. The use according to claim 2, wherein the gastrointestinal hormones comprise motilin, gastrin and somatostatin and the neuropeptides comprise β -endorphin and cholecystokinin octapeptide.

4. The use of claim 1, 2 or 3, wherein the Chinese medicinal composition further comprises refined honey, sorbic acid or potassium sorbate, and the weight parts of the components are as follows: 20-500 parts of astragalus membranaceus, 10-250 parts of fried bighead atractylodes rhizome, 10-250 parts of dried orange peel, 20-500 parts of radix ophiopogonis, 10-250 parts of scutellaria baicalensis, 10-250 parts of fried hawthorn, 10-250 parts of fried radish seed, 80-2500 parts of refined honey and 0.1-5 parts of sorbic acid or potassium sorbate.

5. The use of claim 1, 2 or 3, wherein the Chinese medicinal composition further comprises refined honey, sorbic acid or potassium sorbate, and the weight parts of the components are as follows: 50-300 parts of astragalus membranaceus, 25-150 parts of fried bighead atractylodes rhizome, 25-150 parts of dried orange peel, 50-300 parts of radix ophiopogonis, 25-150 parts of scutellaria baicalensis, 25-150 parts of fried hawthorn, 25-150 parts of fried radish seed, 200-1500 parts of refined honey and 0.3-3 parts of sorbic acid or potassium sorbate.

6. The use of claim 1, 2 or 3, wherein the Chinese medicinal composition further comprises refined honey, sorbic acid or potassium sorbate, and the weight parts of the components are as follows: 66.7 parts of astragalus, 33.3-33.4 parts of fried bighead atractylodes rhizome, 33.3-33.4 parts of dried orange peel, 66.7 parts of dwarf lilyturf tuber, 33.3-33.4 parts of radix scutellariae, 33.3-33.4 parts of fried hawthorn, 33.3-33.4 parts of fried radish seed, 300 parts of refined honey, 0.5 part of sorbic acid or 0.67 part of potassium sorbate.

7. The use as claimed in claim 1, 2 or 3, wherein the radix astragali, rhizoma Atractylodis Macrocephalae, pericarpium Citri Reticulatae, radix Ophiopogonis, radix Scutellariae, fructus crataegi, and semen Raphani are respectively replaced by their extracts;

the extract is water extract or alcohol extract with the volume concentration of 50-90%.

8. Use according to claim 6, characterized in that the molasses is used as excipient, thickener, adjuvant and/or flavouring agent.

9. The use of claim 1, 2 or 3, wherein when the dosage form of the Chinese medicinal composition is liquid, the preparation method comprises the following steps:

(1) weighing radix astragali, fried bighead atractylodes rhizome, dried orange peel, dwarf lilyturf tuber, radix scutellariae, fried hawthorn and fried radish seed according to the parts by weight, adding water, decocting for 2-3 times, each time for 2-3 hours, and combining the decoctions;

(2) concentrating the combined decoction to obtain clear paste with the relative density of 1.01-1.05, refrigerating for 24-48 hours, and filtering to obtain filtrate;

(3) adding refined honey, sorbic acid and/or potassium sorbate into the filtrate in parts by weight, adding water to the total volume of 1-800L, shaking up, standing for 24-48 hours, taking the supernatant, and filtering to obtain the product.

10. The application of claim 9, wherein in the step (1), the decoction is carried out in an extraction tank, the vapor pressure of the extraction tank is less than or equal to 0.15Mpa, the temperature of the extraction liquid medicine is 95-100 ℃, and a liquid medicine circulating pump is started every 20 minutes, and the extraction liquid circulates up and down for 5 minutes;

in the step (2), concentration is carried out in a concentration heater, the vapor pressure of the concentration heater is 0.05-0.15 Mpa, the concentration vacuum degree is 0.06-0.08 Mpa, and paste is collected in a clean area.