CN111003815A - Domestication method of pyridine organic wastewater treatment strains - Google Patents
Domestication method of pyridine organic wastewater treatment strains Download PDFInfo
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- CN111003815A CN111003815A CN201911385845.6A CN201911385845A CN111003815A CN 111003815 A CN111003815 A CN 111003815A CN 201911385845 A CN201911385845 A CN 201911385845A CN 111003815 A CN111003815 A CN 111003815A
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- organic wastewater
- sewage treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Activated Sludge Processes (AREA)
Abstract
The invention provides a method for domesticating pyridine organic wastewater treatment strains, which relates to the technical field of sewage treatment microorganisms and comprises the following steps: 1) adding distilled water into the sludge containing the sewage treatment bacteria, adding dipotassium hydrogen phosphate, acetaldehyde, methanol, magnesium sulfate, sodium acetate, triammonium citrate and manganese sulfate into the sludge, adjusting the COD of mixed effluent to 490-500ppm, and culturing the sewage treatment bacteria for 48-72 hours; 2) adding a proper amount of pyridine organic wastewater to adjust COD to 1500-2000ppm, and culturing the sewage treatment bacteria for 48-96 h; 3) adding a proper amount of pyridine organic wastewater to adjust COD to 4500-5000ppm, and culturing the sewage treatment bacteria for 48-96 h; 4) adding a proper amount of pyridine organic wastewater to adjust COD to 6500-7000ppm, and culturing the sewage treatment bacteria for 48-96 h. The sewage treatment bacteria cultured by the domestication method not only ensures the biochemical activity of the microbial bacteria in the high-concentration organic wastewater, but also can effectively reduce COD, ammonia nitrogen and the like in the pyridine organic wastewater.
Description
Technical Field
The invention relates to the technical field of sewage treatment microorganisms, in particular to a domestication method of pyridine organic wastewater treatment strains.
Background
The sewage treatment strain is derived from nature, a large number of various sewage strains are artificially screened out to obtain high-quality strains in a laboratory, and then the high-quality strains are cultured through a series of domestication to obtain the sewage treatment strain with salt resistance, impact resistance and strong stability. The strain plays a role in repairing life and producing organic pollution of the river course, and conforms to the principles of no toxicity, no public nuisance, no secondary pollution and no harm to human bodies. Can effectively remove ammonia nitrogen, BOD, COD, SS, nitrate radical, sulfate radical, chromaticity, odor, toxic substances, chemical pollutants and the like, and can effectively degrade pollutants in water through pretreatment at the front end of the sewage treatment process and then biochemical anaerobic and aerobic stages. The sweet environment sewage treatment strain solves the problem of sewage treatment.
At present, the domestication method of the strain for treating the sewage of the pyridine organic wastewater is not reported.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides the domestication method of the pyridine organic wastewater treatment strain, and the sewage treatment strain obtained by the domestication method of the invention, which is cultured, not only ensures the biochemical activity of microorganism bacteria in high-concentration organic wastewater, improves the adaptability, impact resistance, stability and salt tolerance of the sewage treatment strain, but also can effectively reduce COD, ammonia nitrogen and the like in the pyridine organic wastewater.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme:
a domestication method of pyridine organic wastewater treatment strains comprises the following steps:
1) adding 900-1100 parts of distilled water into sludge containing sewage treatment bacteria, adding 1-3 parts of dipotassium hydrogen phosphate, 3-10 parts of acetaldehyde, 3-10 parts of methanol, 0.1-0.3 part of magnesium sulfate, 4-6 parts of sodium acetate, 1-3 parts of triammonium citrate, 0.03-0.06 part of manganese sulfate and a certain amount of pyridine organic wastewater, adjusting the COD of mixed effluent to 500ppm of 490-1100-one-phase water, and culturing the sewage treatment bacteria for 48-72 hours;
2) adding a proper amount of pyridine organic wastewater to adjust COD to 1500-2000ppm, and culturing the sewage treatment bacteria for 48-96 h;
3) adding a proper amount of pyridine organic wastewater to adjust COD to 4500-5000ppm, and culturing the sewage treatment bacteria for 48-96 h;
4) adding a proper amount of pyridine organic wastewater to adjust COD to 6500-7000ppm, and culturing the sewage treatment bacteria for 48-96 h.
Further, the water content of the sludge containing the sewage treatment bacteria in the step 1) is 50-75%.
Further, the adding amount of the pyridine organic wastewater in the step 1) is 1-10% of the mass of the sludge.
Further, in the process of domesticating the sewage treatment bacteria, the mixed liquid of the activated sludge and the wastewater needs to be stirred for 16-20 hours, the mixed liquid after stirring is kept stand and is aerated for 2-4 hours, and the dissolved oxygen after aeration is 1.0 +/-0.1 mg/L.
Further, the pH value of the solution is 6.5-8.0 in the acclimation process of the sewage treatment bacteria.
Further, in the acclimatization process of the sewage treatment bacteria, the proportion of carbon, nitrogen and phosphorus in the mixed solution is 1000:5: 1.
(III) advantageous effects
The invention provides a domestication method of pyridine organic wastewater treatment strains, and as the activity of sewage treatment strains is lower under high COD concentration initially, the sewage treatment strains cultured by the domestication method not only ensure the biochemical activity of microorganism bacteria in the high-concentration organic wastewater, improve the adaptability, impact resistance, stability and salt resistance of the sewage treatment strains, but also can effectively reduce COD, ammonia nitrogen and the like in the pyridine organic wastewater.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
a domestication method of pyridine organic wastewater treatment strains comprises the following steps:
1) adding 1000ml of distilled water into 100g of sludge of the sewage treatment bacteria, adding 1.5g of dipotassium phosphate, 3.0g of acetaldehyde, 3.0g of methanol, 0.1g of magnesium sulfate, 4.0g of sodium acetate, 1.0g of triammonium citrate, 0.03g of manganese sulfate and a certain amount of pyridine organic wastewater, adjusting COD (chemical oxygen demand) of mixed effluent to 499mg/L, and culturing the sewage treatment bacteria for 72 hours;
2) adding 120ml pyridine organic wastewater to adjust COD to 1998mg/L, culturing the sewage treatment bacteria for 72h, and reducing the COD of the effluent to 988 mg/L;
3) adding 360ml of pyridine organic wastewater to adjust COD to 4999mg/L, culturing the sewage treatment bacteria for 96h, and reducing the COD of the effluent to 851 mg/L;
4) adding 550ml of pyridine organic wastewater to adjust COD to be 6987mg/L, culturing the sewage treatment bacteria for 96h, and reducing the COD of the effluent to be 985 mg/L.
The COD content of the pyridine organic wastewater in the invention is 20000 mg/L; the water content of the sludge containing the sewage treatment bacteria is 50-75 percent; the adding amount of the pyridine organic wastewater in the step 1) is 3% of the mass of the sludge; in the process of domesticating the sewage treatment bacteria, the mixed liquid of the activated sludge and the wastewater needs to be stirred for 18 hours, the mixed liquid after stirring is kept stand and is aerated by introducing oxygen for 2 hours, and the dissolved oxygen after aeration is 1.0 +/-0.1 mg/L; the pH value of the solution is 6.5-8.0 in the process of domesticating the sewage treatment bacteria; the proportion of carbon, nitrogen and phosphorus in the mixed solution is 1000:5:1 in the acclimation process of the sewage treatment bacteria.
Example 2:
a domestication method of pyridine organic wastewater treatment strains comprises the following steps:
1) adding 150g of sludge containing sewage treatment bacteria into 2000ml of distilled water, adding 2.1g of dipotassium hydrogen phosphate, 3.5g of acetaldehyde, 4.0g of methanol, 0.2g of magnesium sulfate, 8.0g of sodium acetate, 2.0g of triammonium citrate, 0.06g of manganese sulfate and a certain amount of pyridine organic wastewater, adjusting COD (chemical oxygen demand) of mixed effluent to 492mg/L, and culturing the sewage treatment bacteria for 72 hours;
2) adding 300ml of pyridine organic wastewater to adjust COD to 1886mg/L, culturing the sewage treatment bacteria for 72 hours, and reducing the COD of the effluent to 863 mg/L.
3) Adding 700ml pyridine organic wastewater to adjust COD to 4857mg/L, culturing sewage treatment bacteria for 96h, and reducing COD in effluent to 851 mg/L.
4) 1200ml of pyridine organic wastewater is added to adjust COD to 6644mg/L, the sewage treatment bacteria are cultured for 96 hours, and the COD of the effluent is reduced to 985 mg/L.
The COD content of the pyridine organic wastewater in the invention is 20000 mg/L; the water content of the sludge containing the sewage treatment bacteria is 50-75 percent; the adding amount of the pyridine organic wastewater in the step 1) is 6% of the mass of the sludge; in the process of domesticating the sewage treatment bacteria, the mixed liquid of the activated sludge and the wastewater needs to be stirred for 20 hours, the mixed liquid after stirring is kept stand and is aerated by introducing oxygen for 2 hours, and the dissolved oxygen after aeration is 1.0 +/-0.1 mg/L; the pH value of the solution is 6.5-8.0 in the process of domesticating the sewage treatment bacteria; the proportion of carbon, nitrogen and phosphorus in the mixed solution is 1000:5:1 in the acclimation process of the sewage treatment bacteria.
Example 3:
a domestication method of pyridine organic wastewater treatment strains comprises the following steps:
1) adding 120g of sludge containing sewage treatment bacteria into 1400ml of distilled water, adding 2.0g of dipotassium hydrogen phosphate, 6.0g of acetaldehyde, 5.0g of methanol, 0.3g of magnesium sulfate, 8.0g of sodium acetate, 3.0g of ammonium citrate tribasic, 0.05g of manganese sulfate and a certain amount of pyridine organic wastewater, adjusting COD of mixed effluent to 495mg/L, and culturing the sewage treatment bacteria for 72 hours.
2) Adding 140ml pyridine organic wastewater to adjust COD to 1955mg/L, culturing the sewage treatment bacteria for 72h, and reducing the COD of the effluent to 906 mg/L.
3) 380ml of pyridine organic wastewater is added to adjust COD to 4987mg/L, the sewage treatment bacteria are cultured for 96 hours, and the COD of the effluent is reduced to 655 mg/L.
4) Adding 570ml pyridine organic wastewater to adjust COD to be 6988mg/L, culturing the sewage treatment bacteria for 96h, and reducing the COD of the effluent to be 704 mg/L.
The COD content of the pyridine organic wastewater in the invention is 20000 mg/L; the water content of the sludge containing the sewage treatment bacteria is 50-75 percent; the adding amount of the pyridine organic wastewater in the step 1) is 8% of the mass of the sludge; in the process of domesticating the sewage treatment bacteria, the mixed liquid of the activated sludge and the wastewater needs to be stirred for 16 hours, the mixed liquid after stirring is kept stand and is aerated by introducing oxygen for 4 hours, and the dissolved oxygen is 1.0 +/-0.1 mg/L after aeration; the pH value of the solution is 6.5-8.0 in the process of domesticating the sewage treatment bacteria; the proportion of carbon, nitrogen and phosphorus in the mixed solution is 1000:5:1 in the acclimation process of the sewage treatment bacteria.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (6)
1. A domestication method of pyridine organic wastewater treatment strains is characterized by comprising the following steps:
1) adding 2000 parts of 900-2000 parts of distilled water into 150 parts of 100-150 parts of sludge containing sewage treatment bacteria, adding 1-3 parts of dipotassium hydrogen phosphate, 3-10 parts of acetaldehyde, 3-10 parts of methanol, 0.1-0.3 part of magnesium sulfate, 4-8 parts of sodium acetate, 1-3 parts of triammonium citrate, 0.03-0.06 part of manganese sulfate and a certain amount of pyridine organic wastewater, adjusting COD (chemical oxygen demand) of mixed effluent to 500ppm of 490-charge bacteria, and culturing the sewage treatment bacteria for 48-72 hours;
2) adding a proper amount of pyridine organic wastewater to adjust COD to 1500-2000ppm, and culturing the sewage treatment bacteria for 48-96 h;
3) adding a proper amount of pyridine organic wastewater to adjust COD to 4500-5000ppm, and culturing the sewage treatment bacteria for 48-96 h;
4) adding a proper amount of pyridine organic wastewater to adjust COD to 6500-7000ppm, and culturing the sewage treatment bacteria for 48-96 h.
2. The method for domesticating strains of pyridine organic wastewater treatment according to claim 1, wherein the water content of the sludge containing the wastewater treatment strains in step 1) is 50% -75%.
3. The domestication method of pyridine organic wastewater treatment bacteria according to claim 1, wherein the amount of pyridine organic wastewater added in step 1) is 1% -10% of the mass of sludge.
4. The method for acclimatizing pyridine organic wastewater treatment bacteria according to claim 1, wherein the activated sludge wastewater mixed solution is stirred for 16-20 hours during the acclimatization process of the wastewater treatment bacteria, the mixed solution is kept still after stirring and aerated with oxygen for 2-4 hours, and the dissolved oxygen is 1.0 ± 0.1mg/L after aeration.
5. The method for acclimatizing pyridine organic wastewater treatment bacteria according to claim 1, wherein the pH of the solution during the acclimatization process of the wastewater treatment bacteria is 6.5-8.0.
6. The domestication method of pyridine organic wastewater treatment bacteria according to claim 1, wherein the ratio of carbon, nitrogen and phosphorus in the mixed solution is 1000:5:1 in the domestication process of sewage treatment bacteria.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111808751A (en) * | 2020-06-10 | 2020-10-23 | 广州市东鹏食品饮料有限公司 | Microorganism, preparation method thereof and application thereof in organic wastewater treatment |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914441A (en) * | 2010-07-06 | 2010-12-15 | 武汉钢铁(集团)公司 | Method for culturing microbial flora for purifying waste water |
| CN103173400A (en) * | 2013-03-04 | 2013-06-26 | 上海其新生物科技有限公司 | Culturing method of strain used for treating high-concentration tert-butyl alcohol wastewater |
| CN104355403A (en) * | 2014-11-27 | 2015-02-18 | 新疆环境工程技术有限责任公司 | Activated sludge culture acclimatization method for chemical industry wastewater treatment |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914441A (en) * | 2010-07-06 | 2010-12-15 | 武汉钢铁(集团)公司 | Method for culturing microbial flora for purifying waste water |
| CN103173400A (en) * | 2013-03-04 | 2013-06-26 | 上海其新生物科技有限公司 | Culturing method of strain used for treating high-concentration tert-butyl alcohol wastewater |
| CN104355403A (en) * | 2014-11-27 | 2015-02-18 | 新疆环境工程技术有限责任公司 | Activated sludge culture acclimatization method for chemical industry wastewater treatment |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111808751A (en) * | 2020-06-10 | 2020-10-23 | 广州市东鹏食品饮料有限公司 | Microorganism, preparation method thereof and application thereof in organic wastewater treatment |
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Application publication date: 20200414 |