CN111808751A - Microorganism, preparation method thereof and application thereof in organic wastewater treatment - Google Patents
Microorganism, preparation method thereof and application thereof in organic wastewater treatment Download PDFInfo
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- CN111808751A CN111808751A CN202010527610.2A CN202010527610A CN111808751A CN 111808751 A CN111808751 A CN 111808751A CN 202010527610 A CN202010527610 A CN 202010527610A CN 111808751 A CN111808751 A CN 111808751A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/308—Dyes; Colorants; Fluorescent agents
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The invention provides a microorganism, a preparation method thereof and application thereof in organic wastewater treatment, wherein the microorganism is obtained by combining artificial screening and physical mutagenesis, can be used for degrading organic dye wastewater, can degrade naphthalene, basic light yellow, basic orange I, basic orange II and acid yellow 36 through biochemical detection, and can be applied to the treatment of the organic dye wastewater, and when the microorganism is used for the treatment of the wastewater containing the organic dye, the chromaticity and COD of the organic dye wastewater can be effectively reducedCr。
Description
Technical Field
The invention relates to the field of organic wastewater treatment, in particular to a microorganism, a preparation method thereof and application thereof in organic wastewater treatment.
Background
With the rapid development of economy, environmental problems are increasingly prominent. The environmental pollution of a series of sewage discharged by large-scale industrial production, especially the dyeing sewage discharged by the dyeing industry, is particularly obvious, the difficult problem of difficult treatment of the dyeing sewage becomes a worldwide problem in view of the characteristics of high COD, high chroma, high toxicity, difficult biodegradation and the like of the dyeing sewage, the development of the dyeing industry is restricted to a certain extent, and the demand for practically finding a method for solving the difficult problem of difficult treatment of the dyeing sewage is urgent.
At present, the main treatment methods of the wastewater of the dyeing industry and the wastewater of other chemical industries comprise: activated sludge process (most common), biofilm process, reverse osmosis process, ion exchange process. The effect is better by a biological treatment technology, the technical method has low cost and obvious effect, but the existing microorganisms have poor treatment effect on the wastewater in the dyeing industry.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a microorganism, a preparation method thereof and application thereof in organic wastewater treatment.
In order to achieve the purpose, the invention adopts the technical scheme that: a bacterium, the preparation method of the bacterium comprises the following steps:
(1) collecting sludge at a wastewater drainage port in the dyeing industry, and performing amplification culture on original flora in the sludge to obtain a microorganism suspension, wherein a culture medium for the amplification culture is a beef extract peptone culture medium;
(2) inoculating the microorganism suspension obtained by amplification culture to an agar solid culture medium colored by dye sewage, culturing at 22-28 ℃ under the irradiation of ultraviolet rays with the wavelength of 200-300 nm, and screening out microorganisms in a transparent ring with lighter color in the agar solid culture medium;
(3) and (3) diluting the microorganisms obtained in the step (2), inoculating the diluted microorganisms to an agar solid culture medium colored by dye sewage, screening out the microorganisms in a transparent ring with lighter color in the culture medium, and repeatedly screening the microorganisms according to the step (3) to obtain the bacteria.
When the bacteria are used for treating sewage containing organic dye, the chroma and COD of the sewage containing the organic dye can be effectively reducedCr。
Preferably, the beef extract peptone medium comprises the following components in parts by weight: 2.5-3.5 parts of beef extract, 4.5-5.5 parts of peptone, 8-12 parts of sodium chloride, 18-22 parts of agar, 850-950 parts of water and 80-120 parts of dye sewage, wherein the pH value of a beef extract peptone medium is 6.8-7.3.
Preferably, the beef extract peptone medium comprises the following components in parts by weight: 3 parts by weight of beef extract, 5 parts by weight of peptone, 10 parts by weight of sodium chloride, 20 parts by weight of agar, 900 parts by weight of water and 100 parts by weight of dye wastewater, wherein the pH value of the beef extract peptone medium is 7.1.
Preferably, the preparation method of the bacteria comprises the following steps: collecting sludge at a wastewater drainage port in the dyeing industry, and performing amplification culture on an original flora in the sludge to obtain a microorganism suspension, wherein a culture medium for the amplification culture is a beef extract peptone culture medium which comprises the following components: beef extract, peptone, sodium chloride, agar, water and dye sewage, wherein the pH value of a beef extract peptone culture medium is 6.8-7.3, and the beef extract peptone culture medium is sterilized for 20min at 121 ℃ before amplification culture; inoculating the microorganism suspension obtained by amplification culture to an agar solid culture medium colored by dye sewage, culturing for several days in a constant-temperature incubator at the temperature of 25 ℃ under the irradiation of ultraviolet rays of 200-300 nm, screening out the microorganisms in a transparent ring with lighter color in the culture medium, diluting the flora, inoculating the diluted flora to the agar solid culture medium colored by dye sewage again, screening out the microorganisms in the transparent ring with lighter color in the culture medium, and repeating the steps to obtain the bacteria.
The invention also provides a preparation method of any one of the bacteria, which comprises the following steps:
(1) collecting sludge at a wastewater drainage port in the dyeing industry, and performing amplification culture on original flora in the sludge to obtain a microorganism suspension, wherein a culture medium for the amplification culture is a beef extract peptone culture medium;
(2) inoculating the microorganism suspension obtained by amplification culture to an agar solid culture medium colored by dye sewage, culturing at 22-28 ℃ under the irradiation of ultraviolet rays with the wavelength of 200-300 nm, and screening out microorganisms in a transparent ring with lighter color in the agar solid culture medium;
(3) and (3) diluting the microorganisms obtained in the step (2), inoculating the diluted microorganisms to an agar solid culture medium colored by dye sewage, screening out the microorganisms in a transparent ring with lighter color in the culture medium, and repeatedly screening the microorganisms according to the step (3) to obtain any one of the bacteria.
The invention also provides application of any one of the bacteria in degrading organic matters containing benzene ring structures.
The inventors have found through studies that any of the above-mentioned bacteria can degrade an organic substance having a benzene ring structure.
The invention also provides application of any one of the bacteria in degrading naphthalene, basic bright yellow, basic orange I, basic orange II or acid yellow 36.
The inventor finds that any one of the bacteria can degrade naphthalene, basic bright yellow, basic orange I, basic orange II or acid yellow 36, and the structural formula is as follows:
the invention also provides a sewage treatment method containing the organic dye, which comprises the following steps:
mixing the sewage containing the organic dye with any one of the bacteria, wherein the organic dye is an organic matter containing a benzene ring structure.
The inventor finds that any one of the bacteria can effectively reduce the chroma and COD of the organic dye sewage when being used for treating the sewage containing the organic dyeCr。
Preferably, the organic dye-containing wastewater contains at least one compound selected from naphthalene, basic bright yellow, basic orange I, basic orange II, and acid yellow 36.
The invention also provides a microbial inoculum for degrading sewage containing organic dyes, which is prepared by fermenting any one of the bacteria to obtain fermentation liquor, and performing spray drying to obtain a dry powder microbial inoculum.
The invention has the beneficial effects that: the invention provides a microorganism and application thereof in organic wastewater treatment, the microorganism can degrade naphthalene, basic bright yellow, basic orange I, basic orange II or acid yellow 36 through biochemical detection, and can be applied to the treatment of organic dye wastewater, and when the microorganism is used for the treatment of wastewater containing organic dye, the microorganism can effectively reduce the chromaticity and COD of the organic dye wastewaterCr。
Drawings
FIG. 1 is a schematic flow chart of naphthalene degradation by bacteria according to an embodiment of the present invention.
FIG. 2 is a schematic diagram of a process for preparing bacteria according to an embodiment of the present invention.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
Example 1
The screening method of the bacteria for degrading the sewage containing the organic dye comprises the following steps:
(1) collecting 10g of activated sludge containing original flora at a dyeing sewage port;
(2) inoculating the activated sludge containing the original flora in a beef extract peptone culture medium for amplification culture; the beef extract peptone medium formula further comprises the following components: beef extract: 3g, peptone: 5g, sodium chloride: 10g, agar: 20g, water: 900mL and dye wastewater: 100ml, the pH value of the beef extract peptone medium is 7.1, and the beef extract peptone medium is sterilized for 20min at 121 ℃ before amplification culture;
(3) and (3) inoculating the microorganism suspension subjected to amplification culture in the step (2) to an agar solid culture medium colored by dye sewage, culturing for 5 days in a constant-temperature incubator with the temperature set to 25 ℃ under the irradiation of 254nm ultraviolet rays, screening out the microorganisms in a transparent ring with a lighter color in the culture medium, diluting the obtained microorganisms, inoculating the diluted microorganisms to the agar solid culture medium colored by the dye sewage again, screening out the microorganisms in the transparent ring with the lighter color in the culture medium, repeating the steps, and repeatedly screening the microorganisms according to the step (3) to obtain the bacteria for efficiently treating the dyed sewage.
The parameters of the supernatant of the dye wastewater in the step (3) after the step (3) is finished are shown in the following table:
| item | Chroma (double) | CODCr(mg/L) | pH |
| Raw sewage | 1000 | 1527.6 | 7.1 |
| Supernatant fluid | 25 | 60 | 7.0 |
Example 2
The only difference between the screening method for degrading the bacteria containing the organic dye sewage and the embodiment 1 is as follows:
(1) collecting 1000g of activated sludge containing original flora at a dyeing sewage port;
(2) inoculating the original flora in a beef extract peptone culture medium for amplification culture; the beef extract peptone medium formula compound medium: beef extract: 300g, peptone: 500g, sodium chloride: 1000g, agar: 2000g, water: 90L and dye wastewater: 10L, the pH value of the beef extract peptone medium is 7.1, and the beef extract peptone medium is sterilized for 20min at 121 ℃ before amplification culture.
The parameters of the supernatant of the dye wastewater in the step (3) after the step (3) is finished are shown in the following table:
| item | Chroma (double) | CODCr(mg/L) | pH |
| Raw sewage | 900 | 1400 | 7.3 |
| Supernatant fluid | 20 | 50 | 6.9 |
Example 3
The method for treating organic wastewater provided by the embodiment of the invention comprises the following steps: the bacteria selected in example 1 were added to organic wastewater containing naphthalene and mixed well.
After adding the bacteria and mixing, sampling at 0min, 0.5min, 1min, 2min, 5min, 10min, 20min and 1h to detect the concentration of naphthalene in water. The results showed that the naphthalene concentration decreased gradually with the passage of time, indicating that the bacteria selected in example 1 were able to degrade naphthalene. Through research, the scheme of the naphthalene degradation process of the bacteria in example 1 is shown in FIG. 1.
Example 4
The method for treating organic wastewater provided by the embodiment of the invention comprises the following steps: the bacteria selected in example 1 were added to the organic wastewater containing the alkaline light yellow and mixed well.
After adding the bacteria and mixing, sampling and detecting the concentration of the alkaline light yellow in the water at 0min, 0.5min, 1min, 2min, 5min, 10min, 20min and 1 h. The results showed that the concentration of alkaline yellowing gradually decreased with time, indicating that the bacteria selected in example 1 were able to degrade alkaline yellowing.
Example 5
The method for treating organic wastewater provided by the embodiment of the invention comprises the following steps: the bacteria selected in example 1 were added to the organic wastewater containing basic orange and mixed well.
After adding the bacteria and mixing, sampling and detecting the concentration of the basic orange in the water at 0min, 0.5min, 1min, 2min, 5min, 10min, 20min and 1 h. The results showed that the concentration of basic orange was gradually reduced with the passage of time, indicating that the bacteria selected in example 1 were able to degrade basic orange.
Example 6
The method for treating organic wastewater provided by the embodiment of the invention comprises the following steps: the bacteria selected in example 1 were added to the organic wastewater containing basic orange I and mixed well.
After adding the bacteria and mixing, sampling and detecting the concentration of the basic orange I in the water at 0min, 0.5min, 1min, 2min, 5min, 10min, 20min and 1 h. The results show that the concentration of basic orange I gradually decreases with time, indicating that the bacteria selected in example 1 are able to degrade basic orange I.
Example 7
The method for treating organic wastewater provided by the embodiment of the invention comprises the following steps: the bacteria selected in example 1 were added to the organic wastewater containing basic orange II and mixed well.
After adding the bacteria and mixing, sampling and detecting the concentration of the basic orange II in the water at 0min, 0.5min, 1min, 2min, 5min, 10min, 20min and 1 h. The results showed that the concentration of basic orange II decreased gradually over time, indicating that the bacteria selected in example 1 were able to degrade basic orange II.
Example 8
The method for treating organic wastewater provided by the embodiment of the invention comprises the following steps: the bacteria selected in example 1 were added to the organic wastewater containing acid yellow 36 and mixed well.
After adding the bacteria and mixing, sampling and detecting the concentration of acid yellow 36 in water at 0min, 0.5min, 1min, 2min, 5min, 10min, 20min and 1 h. The results showed that the concentration of acid yellow 36 decreased gradually with the passage of time, indicating that the bacteria selected in example 1 were able to degrade acid yellow 36.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (10)
1. The preparation method of the bacterium is characterized by comprising the following steps:
(1) collecting sludge at a wastewater drainage port in the dyeing industry, and performing amplification culture on original flora in the sludge to obtain a microorganism suspension, wherein a culture medium for the amplification culture is a beef extract peptone culture medium;
(2) inoculating the microorganism suspension obtained by amplification culture to an agar solid culture medium colored by dye sewage, culturing at 22-28 ℃ under the irradiation of ultraviolet rays with the wavelength of 200-300 nm, and screening out microorganisms in a transparent ring with lighter color in the agar solid culture medium;
(3) and (3) diluting the microorganisms obtained in the step (2), inoculating the diluted microorganisms to an agar solid culture medium colored by dye sewage, screening out the microorganisms in a transparent ring with lighter color in the culture medium, and repeatedly screening the microorganisms according to the step (3) to obtain the bacteria.
2. The bacterium of claim 1, wherein the beef extract peptone medium comprises the following components in parts by weight: 2.5-3.5 parts of beef extract, 4.5-5.5 parts of peptone, 8-12 parts of sodium chloride, 18-22 parts of agar, 850-950 parts of water and 80-120 parts of dye sewage, wherein the pH value of a beef extract peptone medium is 6.8-7.3.
3. The bacterium of claim 2, wherein the beef extract peptone medium comprises the following components in parts by weight: 3 parts by weight of beef extract, 5 parts by weight of peptone, 10 parts by weight of sodium chloride, 20 parts by weight of agar, 900 parts by weight of water and 100 parts by weight of dye wastewater, wherein the pH value of the beef extract peptone medium is 7.1.
4. A method for producing the bacterium according to any one of claims 1 to 3, comprising the steps of:
(1) collecting sludge at a wastewater drainage port in the dyeing industry, and performing amplification culture on original flora in the sludge to obtain a microorganism suspension, wherein a culture medium for the amplification culture is a beef extract peptone culture medium;
(2) inoculating the microorganism suspension obtained by amplification culture to an agar solid culture medium colored by dye sewage, culturing at 22-28 ℃ under the irradiation of ultraviolet rays with the wavelength of 200-300 nm, and screening out microorganisms in a transparent ring with lighter color in the agar solid culture medium;
(3) diluting the microorganism obtained in the step (2), inoculating the diluted microorganism to an agar solid culture medium colored by dye sewage, screening out the microorganism in a transparent ring with lighter color in the culture medium, and repeatedly screening the microorganism according to the step (3) to obtain the bacterium as claimed in any one of claims 1 to 3.
5. Use of the bacterium according to any one of claims 1 to 3 for degrading organic substances containing benzene ring structures.
6. Use of a bacterium according to any one of claims 1 to 3 for degrading naphthalene, basic bright yellow, basic orange i, basic orange ii or acid yellow 36.
7. The use according to claim 6, wherein the method for degrading naphthalene, basic bright yellow, basic orange I, basic orange II or acid yellow 36 by the bacteria as described in any one of claims 1 to 3 comprises the following steps: mixing the bacterium of any one of claims 1 to 3 with naphthalene, basic bright yellow, basic orange I, basic orange II or acid yellow 36 in water.
8. A method for treating sewage containing organic dyes, characterized in that the method comprises the following steps:
mixing the sewage containing the organic dye with the bacterium as defined in any one of claims 1 to 3, wherein the organic dye is an organic matter containing a benzene ring structure.
9. The method according to claim 8, wherein the organic dye-containing wastewater contains at least one compound selected from the group consisting of naphthalene, basic bright yellow, basic orange I, basic orange II, and acid yellow 36.
10. A microbial inoculum for degrading organic dye-containing sewage, which is characterized in that the strain as claimed in any one of claims 1 to 3 is fermented to obtain fermentation liquor, and the fermentation liquor is spray-dried to obtain a dry powder microbial inoculum.
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Citations (6)
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| KR20010084759A (en) * | 2000-02-29 | 2001-09-06 | 권영순 | Process of dyeing wastewater treatment by Anaerobic-Aerobic-Coagulation |
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| CN103896408A (en) * | 2014-02-13 | 2014-07-02 | 温州科技职业学院 | Method for efficiently degrading azo dye in dye wastewater by using bacteria |
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| CN110510727A (en) * | 2019-09-11 | 2019-11-29 | 辽宁大学 | A method of utilizing ultraviolet oxidation sodium sulfite degradating organic dye |
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2020
- 2020-06-10 CN CN202010527610.2A patent/CN111808751A/en not_active Withdrawn
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|---|---|---|---|---|
| KR20010084759A (en) * | 2000-02-29 | 2001-09-06 | 권영순 | Process of dyeing wastewater treatment by Anaerobic-Aerobic-Coagulation |
| CN101914440A (en) * | 2010-07-06 | 2010-12-15 | 浙江省环境保护科学设计研究院 | Method for massively culturing decolorizing bacteria of dyeing and printing wastewater |
| CN103896408A (en) * | 2014-02-13 | 2014-07-02 | 温州科技职业学院 | Method for efficiently degrading azo dye in dye wastewater by using bacteria |
| CN108395002A (en) * | 2017-02-08 | 2018-08-14 | 广州中国科学院先进技术研究所 | Degradation of Azo Dyes Decolourization Bacteria and its application |
| CN110510727A (en) * | 2019-09-11 | 2019-11-29 | 辽宁大学 | A method of utilizing ultraviolet oxidation sodium sulfite degradating organic dye |
| CN111003815A (en) * | 2019-12-29 | 2020-04-14 | 安徽国星生物化学有限公司 | Domestication method of pyridine organic wastewater treatment strains |
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