CN111000939A

CN111000939A - Essential oil for treating depression and preparation method and application thereof

Info

Publication number: CN111000939A
Application number: CN201911153593.4A
Authority: CN
Inventors: 王恩瀚; 陈汉坤; 叶木英
Original assignee: Guangzhou Qinglan Biotechnology Co ltd
Current assignee: Guangzhou Qinglan Biotechnology Co ltd
Priority date: 2019-11-07
Filing date: 2019-11-07
Publication date: 2020-04-14

Abstract

The invention discloses essential oil for treating depression, and a preparation method and application thereof, and is characterized in that the essential oil is prepared from 290-310 parts of beautiful leopard flower, 200-360 parts of pleione bicolor, 140-160 parts of praecox and 140-160 parts of plumeria rubra by mass. The essential oil raw materials are easy to obtain, and the preparation method is convenient and fast. The results of clinical trials prove that: the essential oil has good effect on treating depression, is easy to be accepted by consumers, and has good application prospect and market prospect.

Description

Essential oil for treating depression and preparation method and application thereof

Technical Field

The invention relates to essential oil and a preparation method and application thereof, in particular to cosmetic essential oil with depression treatment effect and a preparation method and application thereof.

Background

Depression is an affective (mood) disorder caused by various reasons, also known as depressive disorder, which is manifested by mood depression, thought retardation, cognitive impairment and mental hypoactivity, and many patients are accompanied by physical symptoms, and serious ones can lead to suicide. The core symptoms of depression mainly include: decreased interest, depressed mood and insufficient motivation, which may be accompanied by no somatic symptoms and suicidal thoughts or behaviors. WHO studies indicate about 3.5 billion of patients with global depression, and found that more than 20% of the average population in their lives suffer from at least one type of psychiatric disorder, with 6% -15% of patients suicidal death. By 2030, the disease burden of depression will be first. In recent years, the incidence of depression in our country has increased year by year. The 2009 survey result shows that about 4 million of depression patients in China are treated according to clinical regulations, wherein the treatment cost of depression accounts for 5.9% of the total disease cost, and huge mental and economic burden is caused to society and families. The method for treating depression by western medicine usually adopts antidepressant drugs to cooperate with psychological treatment, the curative effect of the antidepressant drugs with single component has certain limitation, the onset period is longer, the antidepressant drugs need to be taken for a long time, most of the antidepressant drugs have adverse reactions with different degrees, patients are easy to generate dependence, and part of the patients have no curative effect reaction on the drugs. There is no name for depression in TCM, and the clinical symptoms of this disease belong to emotional diseases in TCM, similar to ancient books, such as "depression", "lily disease", "hysteria", "globus", "mania", etc. The traditional Chinese medicine has unique advantages in the treatment of depression through accumulation and precipitation in thousands of years of practice. Through a plurality of clinical researches, the curative effect of the traditional Chinese medicine for treating mild and moderate depression is basically the same as that of an antidepressant western medicine, but the traditional Chinese medicine has less adverse reaction and higher safety in treatment, and in conclusion, the development of a novel essential oil product for treating depression with a remarkable effect has a wide market.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provide novel essential oil with depression treatment effect and a preparation process thereof.

The essential oil with depression treatment effect is prepared from raw materials of beautiful leopard flower, pleione bulbocodioides, Stachys praecox and plumeria rubra, wherein the weight percentage of any component is not more than 30% of the total weight.

The essential oil for treating depression is prepared from the following raw materials in parts by mass: 290-310 parts of beautiful leopard flower, 200-260 parts of two-leaf amaranth, 140-160 parts of western region praecox and 140-160 parts of plumeria rubra.

Preferably, the essential oil with the skin moisturizing effect is prepared from the following raw materials in parts by weight: 300 parts of beautiful leopard flower, 250 parts of two-leaf amaranth, 150 parts of western domain praecox and 150 parts of plumeria rubra.

In order to solve the technical problems, the invention also provides a method for preparing the essential oil.

The method for preparing the essential oil comprises the following steps:

(1) ultrahigh pressure treatment: 290-310 parts of beautiful leopard flower, 200-260 parts of two-leaf Dutch, 140-160 parts of Schiff odorata, 140-160 parts of plumeria rubra, and 140-160 parts of plumeria rubra are uniformly mixed, placed in an oven for drying for 24 hours, crushed, sieved by a 60-mesh sieve, then placed in a vacuum packaging bag for vacuum sealing, and then placed in an ultrahigh pressure device of Tianjin Huataisen vast biotechnology limited company, the pressure is set at 300MPa, and the pressure maintaining time is 20min for ultrahigh pressure treatment;

(2) extraction: placing the plant composition in the step (1) in an SFE130-50-02C supercritical fluid extraction device of Huaxing petroleum Co., Ltd. of south China, Jiangsu, as shown in figure 1, setting the flow of CO2 to be 30L/h, the pressure of a separator 1 to be 8MPa, the temperature to be 30 ℃, the pressure of a separator 2 to be 6MPa, the temperature to be 25 ℃, the pressure of the separator 1 to be 4.5MPa, and the temperature to be 15 ℃ to obtain an extract;

(3) molecular distillation separation and purification: dissolving the extract obtained by the supercritical extraction in the step (2) in 50 ℃ absolute ethyl alcohol with the volume 10 times that of the extract, cooling, filtering by using a filter screen with the diameter of 1 mu m to remove most of wax, then carrying out vacuum distillation at 70 ℃ to obtain an ethanol concentrated solution, then placing the ethanol concentrated solution in a KDL5 type wiped film type molecular distillation device of the Germany UIC-Gmb H company, setting the fixed feeding flow rate to be 1mL/min, the wiped film rotation speed to be 150r/min, the distillation temperature to be 90 ℃ and the distillation pressure to be 120Pa, setting the condensation surface temperature to be 4 ℃, and completely removing the ethanol and the water in the concentrated solution. And finally, filling the concentrated solution without the ethanol and the water into a material bottle, and setting separation process parameters as follows: the fixed feeding flow is 1mL/min, the rotating speed of a film scraping is 350r/min, the distillation temperature is 150 ℃, the vacuum degree is 3.0Pa, and the temperature of a condensation surface is 4 ℃ to obtain the refined essential oil.

FIG. 1 schematic diagram of SFE130-50-02C supercritical fluid extraction apparatus

In our country, aromatherapy, also known as aromatherapy, is a natural therapy of using plant essential oils to relieve stress and promote health. The commonly used essential oil can be one or a mixture of several kinds of essential oils to improve the curative effect. Conventional 2-5 monosodium glutamate oil can form a formula, and a finished product is prepared by combining and blending according to the characteristics and chemical properties of various essential oils. There are many ways of treating essential oil, including fumigating, bathing, massaging, scraping, inhaling, and orally taking, through the olfactory, gustatory, and tactile functions of human body, plant hormones are absorbed through skin, digestive system and respiratory system, and the central nervous system, blood circulation, endocrine system, etc. of human body are regulated, so as to restore the coordination of body and mind, and eliminate the feelings of emotion and fatigue such as melancholy, anxiety, vexation, anger, etc. According to literature records and a large number of experimental screens, the essential oil with the effect of treating depression is prepared and obtained by selecting beautiful leopard flower, pleione bicolor, Stachys sieboldii and plumeria rubra as raw materials from a large number of natural plant essential oils, wherein the beautiful leopard flower is dried flower of beautiful leopard flower Nomocharsii basilissa Farrer.ex W.E.Evans of the leopard flower of Liliaceae; the Pleione bulbocodioides L.is dried flower of Pleione bulborum W.W.Smith of Pleione bulbocodioides L.of Pleione of Orchidaceae; the Soprago hispida is selected from Soprago hispida S.himalaicus of Staprago of Stapralidaceae, and dried flower of Soprago hispidus hook.f.et Thoms; the flos Pruni Pseudocerasi is dried leaf of flos Pruni Pseudocerasi of genus Prunus of family Saxifragaceae, Parnassia crassifolia Franchet. All the raw material medicines are subjected to base source identification by a pharmacist in Yuanxiaohong Master, the traditional Chinese medicine of Guangdong province, the quality of all the raw material medicines accords with the use standard, and the sample certificate is stored in a sample chamber of Guangzhou Qinglan Biotechnology Limited company.

According to the literature:

the beautiful leopard flower is a dried flower of a plant of the genus leopard of the family liliaceae, namely the beautiful leopard flower Nomocharysia farrer.

The Pleione bicolor is dried flower of Pleione bicolor Hyssopus cuspidatus Boris of Pleione of Orchidaceae, and is distributed on multiple gravel grass land under coniferous forest, rock wall beside brook or subalpine shrub grassland with elevation above 2500 in northwest of Yunnan and southeast of Tibet. The pleione bicolor belongs to the type of blooming in spring, has unique flower shape, gorgeous flower color and pleasant flower fragrance, and is a precious ornamental flower.

The praecox in western region is praecox of praecox family, is shrub or small arbor, is widely distributed in various places of Yunnan, has white stem marrow, is used as traditional Chinese medicine 'medulla Tetrapanacis' in folk, and has the effects of promoting urination, promoting lactation, clearing damp heat, treating edema, gonorrhea and the like. The praecox flowers in western regions are arranged into spike-shaped inflorescences, the inflorescence stems are short, one large bract and two small bracts are arranged, the large bracts are triangular to nearly triangular, and the small bracts are elliptical. The anther T is born in two chambers, cracks longitudinally, is in an ovary position, has 4 chambers, has more ovules, is born on a midaxial placenta, has a longer style and is in a stigma head shape.

The flos et folium Pruni mume is dried leaf of flos et folium Pruni mume (Panax crassifolia Franch) of genus Meyer of family Saxifragaceae. The plumeria rubra is perennial herb, is 17-55 cm high, upright and strong. The rootstock is thick, nearly spherical or blocky, a plurality of slender roots and mutually interlaced fibrous roots grow downwards, a plurality of membranous brown scales are arranged on the rootstock, and slender stolons grow in axilla of the scales. The plumeria rubra grows at the ditch side with the elevation of 2500 + 3300 meters and in the humid valley.

The depression is used as a multi-occurrence mental disease and has the characteristics of high morbidity, complex pathogenesis, various symptoms, long disease duration and easy relapse. In recent years, scholars at home and abroad find that the traditional Chinese medicine has remarkable clinical effect of preventing and treating the depression, is more and more emphasized by doctors and patients, and plays an irreplaceable role clinically. In summary, the inventor consults a large number of medical monographs and literatures related to depression, screens a plurality of natural plants in sequence, and finally screens the essential oil capable of effectively treating depression. At present, no report about the application of the composition of the beautiful leopard flower, the pleione bulbocodioides, the Stachys sieboldii and the plumeria rubra in treating the depression is found at home and abroad. The essential oil raw materials are easy to obtain, and the preparation method is convenient and fast. The results of clinical trials prove that: the essential oil disclosed by the invention is fragrant in smell, has a good effect on treating depression, is easy to accept by wide consumers, and has a good application prospect and a good market prospect.

The technical solutions of the present invention have been described in detail in the above summary of the invention, and those skilled in the art can repeat the present invention and obtain the technical effects of the present invention based on the above description. The present invention is further explained below by the following detailed description so that the present invention can be more clearly understood by those skilled in the art, and the present invention is not limited thereto, and any equivalent replacement or known modification is within the scope of the present invention.

Detailed Description

Example 1

300 g of beautiful leopard flower, 250 g of two-leaf kadsura longipedunculata, 150 g of western flower grass and 150 g of plumeria rubra are uniformly mixed, placed in an oven for drying for 24h, crushed, sieved by a 60-mesh sieve, then placed in a vacuum packaging bag for vacuum sealing, and then the vacuum bag is placed in an ultrahigh pressure device of Tianjin Huataisen vast biotechnology limited, the pressure is set at 300MPa, and the pressure maintaining time is set for 20min for ultrahigh pressure treatment; placing the plant composition in SFE130-50-02C supercritical fluid extraction device of Huaxing petroleum Co., Ltd, south China, Jiangsu, as shown in FIG. 1, setting CO2 flow rate at 30L/h, pressure at separator 1 at 8MPa, temperature at 30 deg.C, pressure at separator 2 at 6MPa, temperature at 25 deg.C, pressure at separator 1 at 4.5MPa, and temperature at 15 deg.C to obtain extract; then dissolving the extract obtained by supercritical extraction in 50 ℃ absolute ethyl alcohol with the volume 10 times that of the extract, cooling, filtering by a filter screen with the diameter of 1 mu m to remove most of wax, then carrying out vacuum distillation at 70 ℃ to obtain an ethanol concentrated solution, then placing the ethanol concentrated solution in a wiped film type molecular distillation device of KDL5 of the Germany UIC-Gmb H company, setting the fixed feeding flow rate to be 1mL/min, the wiped film rotation speed to be 150r/min, the distillation temperature to be 90 ℃, the distillation pressure to be 120Pa, and the condensation surface temperature to be 4 ℃, and completely removing the ethanol and the water in the concentrated solution. And finally, filling the concentrated solution without the ethanol and the water into a material bottle, and setting separation process parameters as follows: the fixed feeding flow is 1mL/min, the rotating speed of a film scraping is 350r/min, the distillation temperature is 150 ℃, the vacuum degree is 3.0Pa, and the temperature of a condensation surface is 4 ℃ to obtain the refined essential oil. Finally, the essential oil is stored in a brown sealed glass bottle at room temperature and is stored away from light at room temperature.

Example 2

The essential oil obtained in example 1 is used for preparing aromatherapy liquid for treating depression, and the components in percentage by weight are as follows:

the production process comprises the following steps: all the components are stirred evenly at the temperature of 30 ℃.

As described above, the base component used in the present embodiment can optimize the effect of the essential oil of the present invention. However, the conventional substrates which are produced by other manufacturers and can be applied to cosmetics can also be used in the invention, and the dosage of the substrates only meets the dosage standard of national cosmetic additives, and the effect of the invention is not influenced, so the substrates are not listed. The homogenizing and emulsifying equipment used by the invention is an FV-30L FISCO vacuum homogenizing and emulsifying machine produced by Shanghai Fracuk fluid machinery manufacturing Limited company, and has the functions of homogenizing, stirring, temperature control and the like. Other homogeneous emulsifying devices for cosmetic preparation manufactured by other manufacturers can also be used in the invention, and the effects of the invention can be achieved as long as the homogeneous emulsifying devices are operated according to the technological parameters of the invention.

Example 3

In the study, the effect of the essential oil on depression is observed, the curative effect of the essential oil on depression is verified at a human body level, and the material used in the experiment is the aromatherapy liquid prepared in the example 2.

1. Materials and methods

1.1 general data observation cases of depression patients are 80 depression patients who visit the department of Chinese medicine and cantonese and frontier fortunes in Guangzhou in 2016 (3 months) to 2019 (6 months), wherein 36 male patients and 46 female patients are 21.2-40.1 (27.33 +/-8.24) years old. All patients signed informed consent before the experiment began. Random card envelopes are used to group and treat the patients strictly according to the card serial number. Patients meeting inclusion criteria were divided into treatment groups and control groups, 40 each. 18 of 40 patients in the treatment group, 22 of women, the mean age of the patients is (23.91 +/-7.07) years, the mean course of the diseases is (2.35 +/-1.03) years, 18 of 40 patients in the control group, 22 of women, the mean age of the patients is (20.07 +/-7.56) years, the mean course of the diseases is (2.47 +/-0.96) years, and compared with the sex, the age, the course of the diseases and the Hamilton depression scores of the patients in the 2 groups, the difference between the differences without statistical significance is not statistically significant (P > 0.05), and the patients have comparability. The two groups of patients have good compliance, no adverse events occur, and no shedding occurs in the research process.

1.2 diagnostic criteria referring to the diagnostic criteria for depression in "practical internal sciences", patients present with typical symptoms of depressed mood, loss of interest and pleasure, poor energy or fatigue. It is often accompanied by a decrease in concentration, a decrease in self-evaluation, a pessimistic mind, and a sleep disorder. The course of the disease lasts for at least two weeks.

1.3 inclusion criteria 1) compliance with the diagnostic criteria for depression; 2) the Hamilton Depression Scale score is more than or equal to 8 points; 3) the age is 18-50 years old, and the nature is not limited; 4) the patient himself and his immediate relatives agree with the informed consent and sign an informed consent form.

1.4 exclusion criteria 1) those who do not meet the diagnostic criteria; 2) patients with suicidal tendencies; 3) psychotic patients; 4) pregnant and lactating women; 5) the person who can not follow; 6) other drugs were taken which had an effect on the study.

1.5 methods of treatment the aromatherapy liquid used in the treatment group was provided by guangzhou qinglan biotechnology limited, the method of use was that the aromatherapy liquid prepared in example 2 was added into the liquid storage bottle of the K401 aromatherapy machine (guangzhou arnoma environmental protection technology limited), the aromatherapy machine was started at regular time every day, the power was set to 15 w, spraying was started by pressing the spray button for a long time, and 3 hours at regular time/day were used. The control group only adds purified water into the liquid storage bottle of the K401 aromatherapy machine, the other conditions are the same as those of the treatment group, the two groups observe the curative effect after being treated for 60 days, evaluate the curative effect and record the adverse reaction.

1.6 therapeutic effect observation indexes and scoring standards: the evaluation standard of the curative effect is as follows: referring to the classification and diagnosis standard of Chinese mental disorders, the clinical effects are classified into significant effect, effective effect and ineffective effect. The effect is shown: the symptoms completely disappear, the insomnia is obviously improved, and the Hamilton reduction rate is more than or equal to 90 percent; the method has the following advantages: the symptoms are improved, the insomnia is slightly relieved, and the Hamilton reduction rate is 30-89%; and (4) invalidation: no improvement in symptoms, no improvement or aggravation of insomnia, and a Hamilton reduction rate of less than 30%, or death. Hamilton reduction (%) ═ pre-treatment score-post-treatment score// pre-treatment score × 100%. The results were recorded and the total effective rate was calculated, which is (effective + effective)/total number of cases × 100%. The depression state of the patient is evaluated by a Hamilton depression rating scale before and after treatment of Hamilton depression, the rating content comprises 17 problems of sleep onset condition, suicide condition, physical condition and the like, the total score is 0-56, and the higher the score is, the more obvious the depression state of the patient is. If the score is more than or equal to 8 points, the patient is shown to have depression, and the higher the score is, the more serious the disease condition is. Score grading on hamilton depression scale: (1) score < 8, no depression; (2) the score is more than or equal to 8 points, and the mild depression is marked; (3) the score is more than or equal to 17 points, and the patient is moderate depression; (4) score greater than or equal to 24 points, which is considered major depression.

2. Results

After 60 days of treatment, the depression of patients in a treatment group is obviously improved, the total effective rate is 83.33 percent, the total effective rate of a control group is only 30.00 percent, and the total effective rate of the treatment group is obviously higher than that of the control group (P is less than 0.01), which is detailed in table 1.

Table 12 clinical efficacy comparison N (p/%)

Note: p < 0.05, P < 0.01 compared to treatment group.

The difference was not statistically significant (P > 0.05) when compared to the Hamilton Depression Scale scores of two groups of patients before treatment. The Hamilton depression scale score of the treatment group is obviously reduced compared with that before treatment, and the difference has statistical significance (P is less than 0.01) compared with that before treatment. The change of Hamilton depression scale score of the treated group is compared with that of the control group, and the differences have statistical significance (P is less than 0.01), and the difference is shown in a table 2.

TABLE 2 comparison of Hamilton Depression scores for two groups of patients

Note: p < 0.05, P < 0.01, compared to pre-treatment; compared with the control group, the delta P is less than 0.05, and the delta P is less than 0.01.

Adverse reactions did not occur before and after the product safety evaluation treatment, and no 1 patient dropped off. Adverse events related to the aromatherapy liquid containing the essential oil of the invention did not occur in the whole research process.

In conclusion, the cause of depression is complex, the disease course is long, and an effective treatment means is lacked at present. The research result shows that the essential oil can obviously improve the sleep quality of depression patients, has high safety and high clinical application value, and is worthy of popularization and application.

The present invention is not limited to the above-described embodiments, and any variations, modifications, and substitutions which may occur to those skilled in the art may be made without departing from the spirit of the invention.

Claims

1. Essential oil for treating depression, characterized in that: is prepared from beautiful leopard flower, two-leaf pleione, western-style praecox and plumeria rubra by weight percentage of no more than 30% of the total amount.

2. The essential oil for treating depression according to claim 1, which is prepared from the following raw materials in parts by mass: 290-310 parts of beautiful leopard flower, 200-260 parts of two-leaf amaranth, 140-160 parts of western region praecox and 140-160 parts of plumeria rubra.

3. The essential oil for treating depression according to claim 2, which is prepared from the following raw materials in parts by mass: 300 parts of beautiful leopard flower, 250 parts of two-leaf amaranth, 150 parts of western domain praecox and 150 parts of plumeria rubra.

4. The use as claimed in claims 1 to 3, wherein the preparation method of the essential oil comprises the following steps:

(1) ultrahigh pressure treatment: uniformly mixing 290-310 parts of beautiful leopard flower, 200-260 parts of two-leaf amaranth, 140-160 parts of siberian praecox and 140-160 parts of plumeria rubra, drying in an oven for 24 hours, crushing, sieving by a 60-mesh sieve, then placing in a vacuum packaging bag for vacuum sealing, then placing the vacuum bag in ultrahigh pressure equipment of Tianjin Huataisen vast biotechnology limited, setting the pressure to be 300MPa, and keeping the pressure for 20min for ultrahigh pressure treatment;