CN102119956B - Application of lignum dalbergiae odoriferae oil in preparing medicament for resisting human pathogenic bacteria and lignum dalbergiae odoriferae perfume - Google Patents
Application of lignum dalbergiae odoriferae oil in preparing medicament for resisting human pathogenic bacteria and lignum dalbergiae odoriferae perfume Download PDFInfo
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- CN102119956B CN102119956B CN201110046095A CN201110046095A CN102119956B CN 102119956 B CN102119956 B CN 102119956B CN 201110046095 A CN201110046095 A CN 201110046095A CN 201110046095 A CN201110046095 A CN 201110046095A CN 102119956 B CN102119956 B CN 102119956B
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- FQTLCLSUCSAZDY-SZGZABIGSA-N (E)-Nerolidol Natural products CC(C)=CCC\C(C)=C/CC[C@@](C)(O)C=C FQTLCLSUCSAZDY-SZGZABIGSA-N 0.000 description 1
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- Fats And Perfumes (AREA)
Abstract
The invention discloses an application of lignum dalbergiae odoriferae oil in preparing a medicament for resisting human pathogenic bacteria, and also discloses a lignum dalbergiae odoriferae perfume using the lignum dalbergiae odoriferae oil as an active component. The lignum dalbergiae odoriferae perfume disclosed by the invention utilizes the sterilizing efficacy of the lignum dalbergiae odoriferae oil to achieve the purpose of refreshing air, has a simple formula and elegant fragrance, reserves the original unique fragrance of the lignum dalbergiae odoriferae, and simultaneously has the efficacies of sterilizing bacteria and refreshing air, thus having good market development prospects.
Description
Technical field
The present invention relates to the plant extract technical field, the especially new purposes of Lignum Dalbergiae Odoriferae oil in the anti-human body pathogen medicine of preparation, and the Lignum Dalbergiae Odoriferae perfume that utilizes this new purposes to make.
Background technology
Lignum Dalbergiae Odoriferae wingceltis (Dalbergia odorifera T.Chen) is that pulse family (Leguminosae) wingceltis belongs to (Dalbergia) plant; Have another name called rosewood, Lignum Dalbergiae Odoriferae; Be Hainan endemic plant, be distributed in ground such as white sand, east, Le Dong and Sanya, also there is distribution in Fujian, Guangdong, Guangxi now.Lignum Dalbergiae Odoriferae is the trunk of Lignum Dalbergiae Odoriferae wingceltis and the dry duramen of root, is bronzing, and is savory, can do spice usefulness, also can be used as medicine, and is good analgesics.
By the distillable Lignum Dalbergiae Odoriferae oil of Lignum Dalbergiae Odoriferae heartwood, traditional Chinese medical science effect: regulating QI to relieve pain, change silt hemostasis." herbal classic is met former ": " the Lignum Dalbergiae Odoriferae red complexion, go into blood system and descend, thus for oral administration can the promoting the circulation of blood removing blood stasis with potent drugs, be coated with the analgesic therapy of can stopping blooding outward "; Its chemical constituent has β-heerabolene, trans-β-farnesene, trans-nerolidol etc.Nature and flavor temperature, hot has promoting flow of QI and blood, pain relieving, and the hemostatic effect cures mainly diseases such as epigastric pain, hypochondriac pain due to stagnation of liverQI, thoracic obstruction twinge, injury from falling down, traumatic hemorrhage.Therefore be not only famous and precious fixastive, but also have effects such as blood pressure lowering, relaxing muscles and tendons and activating QI and blood in the collateral, resisting coronary heart disease, as fall blood and glue, blood fat reducing, blood pressure lowering, anticoagulant, microcirculation improvement, performances such as portal hypertension fall in calm, analgesia, anti-frightened.Like one Chinese patent application numbers 200610014233.2,200610014238.5,200610014235.1 etc., the purposes of Chinese medicine composition aspect the treatment cardiovascular and cerebrovascular disease that contains Lignum Dalbergiae Odoriferae oil all disclosed.
Summary of the invention
The purposes that the present invention is directed to existing Lignum Dalbergiae Odoriferae oil is single, proposes the new purposes of Lignum Dalbergiae Odoriferae oil in the anti-human body pathogen medicine of preparation.
In order to realize the foregoing invention purpose, the present invention provides following technical scheme: the application of Lignum Dalbergiae Odoriferae oil in the anti-human body pathogen medicine of preparation.
Preferably, said human body pathogen is staphylococcus aureus or methicillin-resistant staphylococcus aureus.
Compared with prior art; Lignum Dalbergiae Odoriferae oil of the present invention is effective in cure aspect anti-human body pathogen disease; Can suppress the growth of staphylococcus aureus and methicillin-resistant staphylococcus aureus effectively; These antibacterial characteristics of Lignum Dalbergiae Odoriferae oil capable of using are made into perfume class the articles for daily use, play the effect of antibacterial, fresh air.
The present invention also provides a kind of Lignum Dalbergiae Odoriferae perfume, is made up of Lignum Dalbergiae Odoriferae oil and the acceptable organic solvent of perfume of 2.4wt%～8.1wt%.
Preferably, said Lignum Dalbergiae Odoriferae oil is 3.8wt%.
Preferably, said organic solvent is the ethanol of volumn concentration 90%～100%.
Preferably, said Lignum Dalbergiae Odoriferae oil is prepared via steam distillation, organic solvent extraction method or supercritical carbon dioxide extraction method by the Lignum Dalbergiae Odoriferae raw material.
Preferably, said organic solvent extraction method comprises following process: get the Lignum Dalbergiae Odoriferae raw material after the pulverizing, add organic solvent to submergence Lignum Dalbergiae Odoriferae raw material; Water-bath reflux, extract, 3 times is extracted 1h～3h at every turn; Filter merging filtrate, the receipts organic solvent is removed in decompression under water-bath, gets Lignum Dalbergiae Odoriferae oil.
Preferably, said organic solvent extraction method comprises following process: get the Lignum Dalbergiae Odoriferae raw material after the pulverizing, add organic solvent to submergence Lignum Dalbergiae Odoriferae raw material; Cold-maceration extracts, and extracts altogether 3 times, extracts 5d～7d at every turn; Filter merge extractive liquid,, under water-bath, remove organic solvent, get Lignum Dalbergiae Odoriferae oil.
Preferably, said organic solvent is petroleum ether, normal hexane, chloroform, ether or ethyl acetate.
Preferably; Said supercritical carbon dioxide extraction method comprises following process: get the Lignum Dalbergiae Odoriferae raw material after the pulverizing, add 95% ethanol submergence Lignum Dalbergiae Odoriferae raw material, adopt supercritical carbon dioxide to extract after placing 24h; Extraction conditions is: extraction column 26MPa～28MPa, and temperature is 30 ℃～35 ℃; Detached dowel 6MPa～7MPa, temperature is 55 ℃～60 ℃, CO
2Flow is 18L/h～20L/h, and the extraction time is 1.5h～2.5h; Get paste, obtain Lignum Dalbergiae Odoriferae oil with organic solvent extraction.
BA result through utilizing Lignum Dalbergiae Odoriferae perfume of the present invention shows, Lignum Dalbergiae Odoriferae perfume of the present invention is to two kinds of human body pathogen: staphylococcus aureus and methicillin-resistant staphylococcus aureus show the obvious suppression effect.
The present invention has initiated the main material that utilizes Lignum Dalbergiae Odoriferae oil effect perfume; Mix the Lignum Dalbergiae Odoriferae perfume that conventional effumability organic solvent makes; Prescription is simple, and fragrant article are graceful, not only kept the original peculiar fragrance of Lignum Dalbergiae Odoriferae; Also have the effect of antibacterial, fresh air simultaneously, have the good prospect of marketing.
The specific embodiment
Describe the present invention below in conjunction with specific embodiment, the description of this part only is exemplary and explanatory, should any restriction not arranged to protection scope of the present invention.
Embodiment 1
Get Lignum Dalbergiae Odoriferae raw material 134g, put among the steam distillation device after it is pulverized, utilize steam distillation to extract, obtain extract Lignum Dalbergiae Odoriferae oil 2.3g.Through detecting, mainly contain oxidation caryophyllene, 7 in the Lignum Dalbergiae Odoriferae oil, compositions such as 11-dimethyl-10-dodecylene-1-alcohol, nerolidol.
The 2.3g Lignum Dalbergiae Odoriferae oil is dissolved in concentration is 90%, weight is in the ethanol of 105g; Alcoholic solution is filtered, remove the insoluble matter in the solution, then gained filtrating is frozen to 5 ℃ of placement 12h, more refrigerated alcoholic solution is filtered, the filtrating behind the secondary filter is returned to room temperature, promptly obtain Lignum Dalbergiae Odoriferae perfume, bottling promptly can be sold.
Embodiment 2
Get Lignum Dalbergiae Odoriferae raw material 127g; It is pulverized the back add extremely just submergence medical material of low boiling petroleum ether (the boiling range temperature of petroleum ether is 30～60 ℃); Adopt the water-bath reflow method to extract then; Extract altogether 3 times: be respectively 1h, 2h and 3h, again extracting solution obtained Lignum Dalbergiae Odoriferae oil 3.7g behind reclaim under reduced pressure petroleum ether solvent under the water-bath.Through detecting, mainly contain oxidation caryophyllene, 7 in the Lignum Dalbergiae Odoriferae oil, compositions such as 11-dimethyl-10-dodecylene-1-alcohol, nerolidol.
The 3.7g Lignum Dalbergiae Odoriferae oil is dissolved in concentration is 95%, weight is in the ethanol of 100g; Alcoholic solution is filtered, remove the insoluble matter in the solution, then gained filtrating is frozen to 5 ℃ of placement 12h, more refrigerated alcoholic solution is filtered, the filtrating behind the secondary filter is returned to room temperature, bottling promptly obtains Lignum Dalbergiae Odoriferae perfume.
Embodiment 3
Get Lignum Dalbergiae Odoriferae raw material 200g; It is pulverized the back add extremely just submergence medical material of low boiling petroleum ether (the boiling range temperature of petroleum ether is 30～60 ℃); Adopt cold-maceration to extract then; Extract altogether 3 times, extract 5d, 7d and 6d respectively, again extracting solution is obtained Lignum Dalbergiae Odoriferae oil 5.3g behind reclaim under reduced pressure petroleum ether solvent under the water-bath.Through detecting, mainly contain oxidation caryophyllene, 7 in the Lignum Dalbergiae Odoriferae oil, compositions such as 11-dimethyl-10-dodecylene-1-alcohol, nerolidol.
The 5.3g Lignum Dalbergiae Odoriferae oil is dissolved in concentration is 90%, weight is in the ethanol of 94g; Alcoholic solution is filtered, remove the insoluble matter in the solution, then gained filtrating is frozen to 5 ℃ of placement 12h, more refrigerated alcoholic solution is filtered, the filtrating behind the secondary filter is returned to room temperature, bottling promptly obtains Lignum Dalbergiae Odoriferae perfume.
Embodiment 4
Get the Lignum Dalbergiae Odoriferae raw material 164g after the pulverizing, add 95% ethanol submergence medical material, carry out supercritical extraction after placing 24h.Extraction conditions is: extraction column 27MPa, and temperature is 34 ℃; Detached dowel 6.8Mpa, temperature is 60 ℃, CO
2Flow is 20L/h, and the extraction time is 2.5h, and Lignum Dalbergiae Odoriferae extractum reuse low boiling petroleum ether (the boiling range temperature of petroleum ether is 30～60 ℃) extraction that said method obtains obtains Lignum Dalbergiae Odoriferae oil 4.7g.Through detecting, mainly contain oxidation caryophyllene, 7 in the Lignum Dalbergiae Odoriferae oil, compositions such as 11-dimethyl-10-dodecylene-1-alcohol, nerolidol.
The 4.7g Lignum Dalbergiae Odoriferae oil is dissolved in concentration is 100%, weight is in the ethanol of 100g; Alcoholic solution is filtered, remove the insoluble matter in the solution, then gained filtrating is frozen to 5 ℃ of placement 12h, more refrigerated alcoholic solution is filtered, the filtrating behind the secondary filter is returned to room temperature, bottling promptly obtains Lignum Dalbergiae Odoriferae perfume.
The activity test of the anti-human body pathogen of Test Example 5 Lignum Dalbergiae Odoriferae perfume
Adopt the Lignum Dalbergiae Odoriferae perfume (being designated as 1,2,3,4 respectively) of embodiment 1～4; Measure its anti-staphylococcus aureus (Staphylococcus aureus) and methicillin-resistant staphylococcus aureus (Methicillin-resistant Staphylococcus aureus, activity MRSA).
It is active to adopt the filter paper agar diffusion method to measure the anti-human body pathogen of Lignum Dalbergiae Odoriferae perfume.S.aureus and MRSA adopt the NA culture medium.S.aureus and MRSA are processed certain density bacteria suspension (10 respectively
5～10
7Cfu/mL), with cotton swab it is evenly coated and to supply the aseptic flat board of examination, process and contain the bacterium flat board.The concentration that then Lignum Dalbergiae Odoriferae perfume is made into is the sample solution of 50mg/mL, gets 50 μ L and drips respectively on the sterilization filter paper that (Φ=6mm) treats after solvent volatilizes filter paper to be affixed on and contains on the bacterium flat board every processing repetition 3 times.S.aureus and MRSA with kanamycin sulfate as positive control.37 ℃ of constant temperature culture of S.aureus and MRSA.Observed result behind the 24h is measured and the record antibacterial circle diameter.
Result of the test shows that the Lignum Dalbergiae Odoriferae perfume of the inventive method preparation shows the obvious suppression effect to two kinds of human body pathogen.
Lignum Dalbergiae Odoriferae perfume to the antibacterial circle diameters of two kinds of pathogen (mm, n=3)
Annotate: the filter paper dick diameter is 6mm.Kanamycin sulfate is done positive control.What blank used is 100% ethanol.
It below only is preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (1)
1. the application of Lignum Dalbergiae Odoriferae oil in anti-staphylococcus aureus of preparation and methicillin-resistant staphylococcus aureus medicine.
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| CN201110046095A CN102119956B (en) | 2011-02-25 | 2011-02-25 | Application of lignum dalbergiae odoriferae oil in preparing medicament for resisting human pathogenic bacteria and lignum dalbergiae odoriferae perfume |
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| CN201110046095A CN102119956B (en) | 2011-02-25 | 2011-02-25 | Application of lignum dalbergiae odoriferae oil in preparing medicament for resisting human pathogenic bacteria and lignum dalbergiae odoriferae perfume |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210264954.4A Division CN102755276B (en) | 2011-02-25 | 2011-02-25 | Dalbergia wood perfume |
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| CN102119956B true CN102119956B (en) | 2012-09-05 |
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| CN104940971A (en) * | 2015-07-23 | 2015-09-30 | 林贻强 | Natural vehicle-mounted aroma powder |
| CN106726895A (en) * | 2016-12-06 | 2017-05-31 | 蔡日芬 | A kind of Huanghua Pear eyeshield frost and preparation method thereof |
| CN116712481A (en) * | 2023-07-27 | 2023-09-08 | 中科万康药业(广州)有限公司 | Sustained-release antibacterial composition and application thereof |
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| CN101023983B (en) * | 2006-02-23 | 2011-12-21 | 山东绿叶天然药物研究开发有限公司 | Medicine composition and use |
| CN101439076B (en) * | 2007-11-22 | 2012-04-18 | 天津天士力制药股份有限公司 | Preparation method of chinese medicine granule containing dalbergia heartwood oil |
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Non-Patent Citations (2)
| Title |
|---|
| 刘海燕等.降香挥发油的研究进展.《中草药》.2009,第40卷(第11期), * |
| 周吴萍等.中药降香研究概况.《安徽农业科学》.2010,第38卷(第26期), * |
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