CN110982672A - Stem cell separation device - Google Patents
Stem cell separation device Download PDFInfo
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- CN110982672A CN110982672A CN202010014191.2A CN202010014191A CN110982672A CN 110982672 A CN110982672 A CN 110982672A CN 202010014191 A CN202010014191 A CN 202010014191A CN 110982672 A CN110982672 A CN 110982672A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The invention relates to a stem cell separation device, which comprises a shell, a separator and a cleaner, wherein a separation chamber is formed in the separator, a cleaning solution storage chamber is formed in the cleaner, and a discharge solution storage chamber is formed between the shell and the separator as well as between the shell and the cleaner. When the device is matched with a centrifugal machine for stem cell separation, the separation chamber can complete the layering of corresponding mixed liquid and the discharge of waste liquid under the centrifugal action, so that stem cell liquid is separated. And under the centrifugal action, the cleaning solution in the cleaning device flows into the separation chamber, the stem cell solution in the separation chamber is cleaned for multiple times, and the cleaned cleaning solution containing the collagenase is discharged to the discharge solution storage chamber. When the stem cell separation device is used, only the cleaning solution and the adipose tissue mixed solution are needed to be respectively injected into the cleaning device and the separator, and then the cleaning solution and the adipose tissue mixed solution are put into the centrifuge, so that the stem cell separation device is free from multiple times of manual liquid taking and adding, and is simple and convenient to operate. And because the liquid is not required to be taken and added manually for many times, the stem cell liquid can be prevented from being damaged and polluted.
Description
Technical Field
The invention relates to the field of medical instruments, in particular to a stem cell separation device.
Background
Stem cells are primitive cells having self-renewal and multipotential differentiation potential, which are cells of origin of the body and can differentiate into one or more cells constituting human tissues or organs under specific conditions. Adipose-derived stem cells are recently isolated from adipose tissues as stem cells having a multi-differentiation potential, which can be differentiated into osteoblasts, chondroblasts, adipocytes, etc. under specific induction conditions.
In the prior art, the operation of separating stem cells from adipose tissue is generally as follows: placing the fat tissue decomposed by collagenase into a separator, and separating a cell layer, an oil layer and a waste liquid layer in the fat tissue in a centrifugal mode due to different densities of components in the fat tissue; and subjected to repeated centrifugation at least twice. And because the separation operation needs to be repeated for a plurality of times to manually take and add liquid, the mechanical damage of cells is easily caused and the cells are easily polluted, thereby influencing the cell quality and increasing the cell separation cost.
In view of the above, the present inventors have conducted extensive research and development experiments to solve the problems and disadvantages of the conventional stem cell separation apparatus.
Disclosure of Invention
In view of the problems of the prior art, the present invention aims to provide a stem cell separation device which is convenient to operate.
In order to achieve the purpose, the invention adopts the technical scheme that:
a stem cell separation device, comprising: comprises a shell, a separator and a cleaner which are arranged in the shell,
the cleaner comprises a cleaner shell and a cleaning piston which is arranged in the cleaner shell and is hermetically connected with the cleaner shell, wherein the upper end of the cleaning piston is matched with the cleaner shell to form a cleaning solution storage chamber;
the separator comprises a separator shell, a separation piston and a spring, wherein the separator piston and the spring are arranged in the separator shell, one end of the spring is connected with the separation piston, and the other end of the spring is connected with the bottom of the separator shell; the separating piston is connected with the separator shell in a sealing way, and a separating chamber is formed between the separating piston and the separator shell;
a discharged liquid storage chamber is formed among the shell, the cleaner shell and the separator shell, and the discharged liquid storage chamber, the washing liquid storage chamber and the separation chamber are all sealed chambers;
the shell is provided with a first injection port, a second injection port, an extraction port, a waste liquid discharge channel and a waste liquid discharge port; the first injection port is communicated with the cleaning solution storage chamber, and the waste liquid discharge port is communicated with the discharge liquid storage chamber; the second injection port and the extraction port are communicated with the separation chamber, and one-way valves for preventing liquid in the separation chamber from flowing back are arranged in the second injection port and the extraction port; one end of the waste liquid discharge channel is communicated with the separation chamber, and the other end of the waste liquid discharge channel is communicated with the discharge liquid storage chamber; the waste liquid discharge channel is provided with a centrifugal valve for controlling the waste liquid discharge channel to be opened or closed and a one-way valve for preventing liquid in the discharge liquid storage chamber from flowing back.
The waste liquid discharge channel is provided with a channel inlet and a channel outlet, the channel inlet is communicated with the separation chamber, and the channel outlet is communicated with the discharge liquid storage chamber; the centrifugal valve is arranged at the inlet of the channel, and the one-way valve is arranged at the outlet of the channel.
Protective caps are arranged on the first injection port, the second injection port and the extraction port.
The first injection opening and the second injection opening are connected through a conveying pipe, and the two ends of the conveying pipe are respectively connected with the first injection opening protective cap and the second injection opening protective cap.
The separator shell bottom is equipped with a spring and carries, the spring is connected on this spring carries.
A hose is arranged in the cleaner shell and connected with the first filling opening.
The upper end of the separation piston is provided with a conical collection space.
After the scheme is adopted, the stem cell separation device comprises a shell, a separator and a cleaner, wherein a separation chamber is formed in the separator, a cleaning solution storage chamber is formed in the cleaner, and a discharge solution storage chamber is formed between the shell and the separator as well as between the shell and the cleaner. When the device is matched with a centrifugal machine for stem cell separation, the separation chamber can complete the layering of corresponding mixed liquid and the discharge of waste liquid under the centrifugal action, so that stem cell liquid is separated. And under the centrifugal action, the cleaning solution in the cleaning device flows into the separation chamber, the stem cell solution in the separation chamber is cleaned for multiple times, and the cleaned cleaning solution containing the collagenase is discharged to the discharge solution storage chamber. When the stem cell separation device is used, only the cleaning solution and the adipose tissue mixed solution are needed to be respectively injected into the cleaning device and the separator, and then the cleaning solution and the adipose tissue mixed solution are put into the centrifuge, so that the stem cell separation device is free from multiple times of manual liquid taking and adding, and is simple and convenient to operate. And because the liquid is not required to be taken and added manually for many times, the stem cell liquid can be prevented from being damaged and polluted.
Drawings
FIG. 1 is a schematic view of the assembly structure of the separation device of the present invention;
FIG. 2 is an exploded view of the separator of the present invention;
FIG. 3 is a cross-sectional view of the separating apparatus of the present invention with cleaning solution injected;
FIG. 4 is a cross-sectional view of the separation device of the present invention as it is being injected into adipose tissue;
FIG. 5 is a cross-sectional view of the separation device during centrifugation according to the present invention.
Detailed Description
As shown in fig. 1 to 3, the present invention discloses a stem cell separation device, which includes a housing 100, and a separator 200 and a washer 300 disposed in the housing 100.
The cleaner 300 includes a cleaner housing 310 and a cleaning piston 320 disposed in the cleaner housing 310 and hermetically connected to the cleaner housing 310, wherein an upper end of the cleaning piston 320 cooperates with the cleaner housing to form a cleaning solution storage chamber 330.
The separator 200 comprises a separator shell 210, a separating piston 220 and a spring 230, wherein the separator 200 piston and the spring 230 are arranged in the separator shell 210, one end of the spring 230 is connected with the separating piston 220, and the other end of the spring is connected with the bottom of the separator shell 210; the separation piston 220 is sealingly connected to the separator housing 210, and a separation chamber 250 is formed therebetween. The bottom of the separator housing 210 is provided with a spring mount 240, and the spring 230 is connected to the spring mount 240. In order to achieve a better separation effect of the separator 200, a collection space having a conical shape is formed at the upper end of the separation piston 220.
The casing 100, the washer housing 310 and the separator housing 210 form a drain storage chamber 160 therebetween, and the drain storage chamber 160, the cleaning solution storage chamber 330 and the separation chamber 250 are sealed chambers.
The housing 100 is provided with a first inlet 110, a second inlet 120, an extraction port 130, a waste liquid discharge passage 140, and a waste liquid discharge port 150. The waste liquid discharge port 150 and a discharge liquid reservoir 160. The first inlet 110 is connected to the cleaning solution storage chamber 330, the first inlet 110 is provided with a first protective cap 111, and when the separating device is in a rest state, the first protective cap 111 covers the first inlet 110 to prevent external contamination. A hose 340 is disposed in the cleaner housing 310, and the hose 340 is connected to the first injection port 110.
The second injection port 120 and the extraction port 130 are communicated with the separation chamber 250, a first one-way valve 121 for preventing the liquid in the separation chamber 250 from flowing back from the second injection port 120 is arranged in the second injection port 120, and a second protective cap 122 is arranged on the second injection port 120, wherein the second protective cap 122 is also used for protecting the second injection port 120 from external pollution. The second check valve 131 is disposed in the extraction port 130, and the liquid in the separation chamber 250 is prevented from flowing back from the extraction port 130 by the second check valve 131. The extraction opening 130 is further provided with a third protective cap 132, and the third protective cap 132 can protect the extraction opening 130 from outside contamination.
One end of the waste liquid discharge channel 140 communicates with the separation chamber 250, and the other end communicates with the discharge liquid storage chamber 160; the waste liquid discharge channel 140 is provided with a centrifugal valve 141 for controlling the opening or closing of the waste liquid discharge channel 140, and a fourth check valve 142 for preventing the backflow of the liquid in the discharged liquid storage chamber 160. Specifically, the waste liquid discharge channel 140 is provided with a channel inlet communicating with the separation chamber 250 and a channel outlet communicating with the discharge liquid reservoir chamber 160; the centrifugal valve 141 is arranged at the inlet of the channel, and the fourth check valve 142 is arranged at the outlet of the channel.
When the device is used, the first injection port 110 and the second injection port 120 need to be communicated, and for convenience of use, the first injection port 110 and the second injection port 120 are communicated through a delivery pipe 170, and the first protective cap 111 and the second protective cap 122 are respectively connected to two ends of the delivery pipe 170.
The stem cell extraction device is mainly used for extracting stem cells from adipose tissues and can also be used for extracting other types of cells. When the stem cell extraction device is used for extracting stem cells from adipose tissues, the stem cell extraction device is matched with a centrifuge for use. The method comprises the following specific steps:
as shown in fig. 3, a cleaning solution (physiological saline) is injected into the cleaner 300 through the first injection port 110 by a syringe.
As shown in fig. 4, the collagenase decomposed adipose tissue is injected into the separator 200 through the second injection port 120 using a syringe.
The delivery pipe 170 is connected to the first injection port 110 and the second injection port 120.
The centrifugal valve 141 is opened, the extraction device is placed in a centrifuge, and after the door of the centrifuge is closed, centrifugation is performed according to a program set by the centrifuge.
The setting procedure was as follows:
1. accelerating the centrifuge to 1360rpm, and decelerating the centrifuge to 200rpm after centrifuging for 3 minutes;
2. accelerating to 1360rpm, centrifuging for 3 minutes, and decelerating to 200 rpm;
3. accelerating to 1360rpm, centrifuging for 3 minutes, and decelerating to 200 rpm;
4. accelerated to 1360rpm, centrifuged for 3 minutes and then decelerated to 200 rpm.
After centrifugation is finished, extracting stem cell layer liquid through an extraction port 130 by using an injector; the waste liquid in the discharge liquid reservoir 160 is discharged through the waste liquid discharge port 150.
During the above centrifugation, the spring 230 is compressed downward by the centrifugal force, thereby moving the separation piston 220 downward. Downward movement of the separation piston 220 causes a negative pressure to be formed in the separation chamber 250, since the separation chamber 250 is in communication with the cleaning solution storage chamber 330 via the delivery tube 170. Therefore, when a negative pressure is formed in the separation chamber 250, the cleaning liquid in the cleaning liquid storage chamber 330 enters the separation chamber 250 through the first inlet 110, the delivery pipe 170 and the second inlet 120 (as shown in fig. 5), and the cleaning piston 320 moves upwards. Due to the different density of the main components in the mixed liquid of adipose tissues in the separation chamber 250, the mixed liquid of adipose tissues in the separation chamber 250 is layered under the centrifugal force. When the centrifugation is stopped, the separation piston 220 moves upward by the elastic force of the spring 230, and the liquid in the upper layer in the separation chamber 250 is discharged into the discharged liquid storage chamber via the waste liquid discharge channel.
During the first centrifugation, the adipose tissue mixed solution in the separation chamber 250 is layered into a stem cell layer, an oil layer, and a waste liquid layer. When the centrifugation is stopped, the spring 230 pushes the separation piston 220 to move up, and the grease layer, the waste liquid layer, and a small amount of stem cell layer are discharged from the waste liquid discharge channel 140 into the discharge liquid storage chamber 160.
The second to fourth centrifugations are to wash the cell fluid by the washing solution to wash away the residual collagenase in the cell fluid, so as to obtain purer cell fluid. When the negative pressure is formed in the separation chamber 250, the cleaning solution in the cleaner 300 is injected into the separator 200 to be mixed with the stem cell solution, and then centrifugal separation is performed. When the centrifugation is stopped, the spring 230 pushes the separation piston 220 to move up, and the collagenase mixed in the upper layer is discharged to the discharged liquid storage chamber 160.
In summary, the stem cell separation device of the present invention includes a housing 100, a separator 200, and a washer 300, wherein a separation chamber 250 is formed in the separator 200, a cleaning solution storage chamber 330 is formed in the washer 300, and a discharge solution storage chamber 160 is formed between the housing 100 and the separator 200, and the washer 300. When the device is used with a centrifuge for stem cell separation, the separation chamber 250 can complete the layering of the corresponding mixed liquid and the discharge of waste liquid under the centrifugal action, thereby separating out stem cell liquid. The washing liquid in the washer 300 flows into the separation chamber 250 by centrifugation, the stem cell liquid in the separation chamber 250 is washed a plurality of times, and the washed washing liquid containing the collagenase is discharged to the discharge liquid storage chamber. When the stem cell separation device is used, only the cleaning solution and the mixed liquid of the adipose tissues are needed to be respectively injected into the cleaning device 300 and the separator 200 and then placed into the centrifuge, so that the liquid taking and adding are not needed to be manually carried out for many times, and the operation is simple and convenient. And because the liquid is not required to be taken and added manually for many times, the stem cell liquid can be prevented from being damaged and polluted.
The above description is only exemplary of the present invention and is not intended to limit the technical scope of the present invention, so that any minor modifications, equivalent changes and modifications made to the above exemplary embodiments according to the technical spirit of the present invention are within the technical scope of the present invention.
Claims (7)
1. A stem cell separation device, comprising: comprises a shell, a separator and a cleaner which are arranged in the shell,
the cleaner comprises a cleaner shell and a cleaning piston which is arranged in the cleaner shell and is hermetically connected with the cleaner shell, wherein the upper end of the cleaning piston is matched with the cleaner shell to form a cleaning solution storage chamber;
the separator comprises a separator shell, a separation piston and a spring, wherein the separator piston and the spring are arranged in the separator shell, one end of the spring is connected with the separation piston, and the other end of the spring is connected with the bottom of the separator shell; the separating piston is connected with the separator shell in a sealing way, and a separating chamber is formed between the separating piston and the separator shell;
a discharged liquid storage chamber is formed among the shell, the cleaner shell and the separator shell, and the discharged liquid storage chamber, the washing liquid storage chamber and the separation chamber are all sealed chambers;
the shell is provided with a first injection port, a second injection port, an extraction port, a waste liquid discharge channel and a waste liquid discharge port; the first injection port is communicated with the cleaning solution storage chamber, and the waste liquid discharge port is communicated with the discharge liquid storage chamber; the second injection port and the extraction port are communicated with the separation chamber, and one-way valves for preventing liquid in the separation chamber from flowing back are arranged in the second injection port and the extraction port; one end of the waste liquid discharge channel is communicated with the separation chamber, and the other end of the waste liquid discharge channel is communicated with the discharge liquid storage chamber; the waste liquid discharge channel is provided with a centrifugal valve for controlling the waste liquid discharge channel to be opened or closed and a one-way valve for preventing liquid in the discharge liquid storage chamber from flowing back.
2. A stem cell separation device according to claim 1, wherein: the waste liquid discharge channel is provided with a channel inlet and a channel outlet, the channel inlet is communicated with the separation chamber, and the channel outlet is communicated with the discharge liquid storage chamber; the centrifugal valve is arranged at the inlet of the channel, and the one-way valve is arranged at the outlet of the channel.
3. A stem cell separation device according to claim 1, wherein: protective caps are arranged on the first injection port, the second injection port and the extraction port.
4. A stem cell separation device according to claim 3, wherein: the first injection opening and the second injection opening are connected through a conveying pipe, and the two ends of the conveying pipe are respectively connected with the first injection opening protective cap and the second injection opening protective cap.
5. A stem cell separation device according to claim 1, wherein: the separator shell bottom is equipped with a spring and carries, the spring is connected on this spring carries.
6. A stem cell separation device according to claim 1, wherein: a hose is arranged in the cleaner shell and connected with the first filling opening.
7. A stem cell separation device according to claim 1, wherein: the upper end of the separation piston is provided with a conical collection space.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010014191.2A CN110982672A (en) | 2020-01-07 | 2020-01-07 | Stem cell separation device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010014191.2A CN110982672A (en) | 2020-01-07 | 2020-01-07 | Stem cell separation device |
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| Publication Number | Publication Date |
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| CN110982672A true CN110982672A (en) | 2020-04-10 |
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| CN202010014191.2A Pending CN110982672A (en) | 2020-01-07 | 2020-01-07 | Stem cell separation device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112375654A (en) * | 2020-11-20 | 2021-02-19 | 广州穗阳生物学研究有限公司 | Adipose-derived stem cell separation device and method |
-
2020
- 2020-01-07 CN CN202010014191.2A patent/CN110982672A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112375654A (en) * | 2020-11-20 | 2021-02-19 | 广州穗阳生物学研究有限公司 | Adipose-derived stem cell separation device and method |
| CN112375654B (en) * | 2020-11-20 | 2023-12-05 | 广州穗阳生物学研究有限公司 | Adipose-derived stem cell separation device and separation method |
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Effective date of registration: 20200529 Address after: 361000 unit 82, floor 1, No. 2036, wengjiao West Road, Haicang District, Xiamen City, Fujian Province (building A19, Xiamen biomedical industrial park) Applicant after: Xiamen Bosen Regenerative Medicine Engineering Co., Ltd Applicant after: Weikan (Xiamen) Medical Technology Co.,Ltd. Address before: Unit 12, floor 1, No. 2036, wengjiao West Road, Haicang District, Xiamen City, Fujian Province Applicant before: Weikan (Xiamen) Medical Technology Co.,Ltd. |
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Effective date of registration: 20210825 Address after: 361000 units 01-2, 03 and 04, 5th floor, building B14, Xiamen biomedical industrial park, 2076 wengjiao West Road, Haicang District, Xiamen City, Fujian Province Applicant after: Xiamen Bosen Regenerative Medicine Engineering Co., Ltd Address before: 361000 unit 82, floor 1, No. 2036, wengjiao West Road, Haicang District, Xiamen City, Fujian Province (building A19, Xiamen biomedical industrial park) Applicant before: Xiamen Bosen Regenerative Medicine Engineering Co., Ltd Applicant before: Weikan (Xiamen) Medical Technology Co.,Ltd. |