CN219356288U - Efficient separation test tube for cell tissue fluid - Google Patents

Abstract

The utility model discloses a test tube for efficiently separating cell and tissue fluid, which comprises: a tube body, a cover body, a liquid outlet mechanism, a pressure relief mechanism and a suction tube; Partition; the cover is set on the opening of the pipe body; the liquid outlet mechanism is arranged on the partition, and the first cavity and the second cavity are communicated through the liquid outlet mechanism; the pressure relief mechanism is set On the upper part of the cover body; one end of the suction pipe is connected with the liquid outlet mechanism, and the other end of the suction pipe is arranged at the bottom of the pipe body. In the utility model, the deposition liquid enters the second cavity through the liquid outlet hole, and when the deposition liquid enters the second cavity, the air inlet hole is opened to balance the air pressure in the second cavity. At the bottom of the tube body, stop pumping to complete the separation of tissue fluid. During this process, the supernatant and sedimentation fluid can be separated more thoroughly, reducing contact with the outside world, and preventing contact with the inside of the test tube to avoid contamination.

Description

A test tube for efficient separation of cell and tissue fluid

technical field

The utility model relates to the field of cell tissue fluid separation, in particular to a test tube for efficiently separating cell tissue fluid.

Background technique

The composition of body fluid includes extracellular fluid and intracellular fluid. The composition of extracellular fluid includes: cells, tissue fluid and lymphocytes. In the experiment on cells, it is necessary to use mechanical or enzymatic methods to disperse the tissue into individual cells, wash , centrifuge, collect the precipitate, and then sonicate, centrifuge and layer, and then extract the tissue fluid, and then use the remaining layered cells for the experiment.

The patent document (CN213245011U) provides a double-chamber centrifuge tube that integrates the functions of suspension separation and supernatant extraction. By arranging a partition in the test tube, an elastic one-way valve is arranged on the partition, and the test tube is vertically separated. The chamber is divided into a sample chamber and a supernatant collection chamber. The elastic one-way valve is provided with a separation hole, a baffle, a mass block, and an elastic structure. As long as the double-chamber centrifuge tube is inserted into the fixed-angle rotor of the centrifuge, the position of the sample chamber and the supernatant collection chamber can be changed. Realize the separation of the liquid area inside the centrifuge tube. The present invention uses centrifugal force as the switch for opening and closing the one-way valve to realize the separation and extraction of the supernatant, and the method is extremely efficient, fast and pure when applied to occasions where the supernatant needs to be extracted.

In the above public documents, the one-way valve switch is opened by centrifugation to separate and extract the supernatant, but there are also the following disadvantages:

(1) Under the action of centrifugal force, the one-way valve is opened to allow the supernatant liquid to enter the collection chamber, but only part of the supernatant liquid enters the collection chamber during actual operation, and cannot be completely separated.

(2) Manual separation is still required between the supernatant liquid and the deposition liquid remaining in the sample chamber.

Utility model content

An object of the present utility model is to provide a test tube for efficiently separating cell and tissue fluid that at least solves any aspect of the above-mentioned technical problems.

A further object of the present invention is to avoid that the supernatant remains mixed with the sedimentation liquid.

Another further purpose of the utility model is to improve the separation degree of sedimentation liquid and supernatant liquid.

In particular, the utility model provides a test tube for efficient separation of cell and tissue fluid, including: a tube body, a cover, a liquid outlet mechanism, a pressure relief mechanism, and a suction tube. Partition of the second cavity; the cover is set on the opening of the tube; the liquid outlet mechanism is set on the partition, and the first cavity and the second cavity pass through the The liquid outlet mechanism is connected; the pressure relief mechanism is arranged on the upper part of the cover body; one end of the suction pipe is connected with the liquid outlet mechanism, and the other end of the suction pipe is arranged at the bottom of the pipe body.

Further, the section of the partition is "L".

Further, the liquid outlet mechanism is arranged on the partitioned transverse partition, the liquid outlet mechanism includes: a liquid outlet hole, a fixed frame, a valve plate and a spring, and a movable chamber is arranged in the liquid outlet hole; The fixing frame is arranged on the upper part of the movable chamber; the valve plate is against the opening of the liquid outlet hole towards the second cavity; the spring is arranged between the fixing frame and the valve plate.

Further, the diameter of the active chamber is larger than the diameter of the liquid outlet hole.

Further, the straw is arranged close to the side wall of the tube body.

Further, the pressure relief mechanism includes: an air inlet and an air inlet, the air inlet is facing the first cavity, and a cork is arranged in the air inlet; the air inlet is facing the first cavity. Two cavities, the air inlet is provided with a one-way air inlet valve.

Further, the soft plug is a rubber plug.

Technical effect and advantage of the present utility model:

1. The utility model divides the test tube into the first cavity and the second cavity, and injects the blood to be separated into the second cavity. After centrifugation, the blood is layered, the upper part is the supernatant, and the lower part is the sedimentary liquid , connect the needle tube to the first perforation to pump air, at this time, the air pressure in the second cavity becomes smaller, and the valve plate against the liquid outlet hole opens, and the deposition liquid enters the second cavity through the liquid outlet hole, and deposits When the liquid enters the second cavity, the air inlet hole is opened to balance the air pressure in the second cavity. When the supernatant liquid completely fills the bottom of the tube body, the suction is stopped to complete the separation of tissue fluid. It can make the supernatant and sedimentation liquid separate more thoroughly, reduce the contact with the outside world, and prevent the inside of the test tube from being polluted.

2. After the separation is completed, the utility model can be directly stored without opening, which is convenient to use.

Description of drawings

Hereinafter, some specific embodiments of the present utility model will be described in detail in an exemplary rather than restrictive manner with reference to the accompanying drawings. The same reference numerals in the drawings designate the same or similar parts or parts. Those skilled in the art will appreciate that the drawings are not necessarily drawn to scale. In the attached picture:

Fig. 1 is the structural representation of the utility model.

Fig. 2 is a schematic diagram of the structure of the utility model without a cover.

Fig. 3 is a schematic diagram of the front view structure of the utility model.

Fig. 4 is a schematic diagram of the sectional structure along the A-A direction in Fig. 3 of the present invention.

Fig. 5 is a partial enlarged structural schematic diagram of B in Fig. 4 of the present utility model.

In the figure: 1, pipe body, 101, first cavity, 102, second cavity, 103, partition, 2, cover, 201, air suction port, 202, air inlet, 3, liquid outlet mechanism, 301, Liquid outlet, 302, fixed frame, 303, spring, 304, valve plate, 305, diversion groove, 306, movable cavity, 4, suction pipe.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

Fig. 1 is the structural representation of the utility model. Fig. 2 is a schematic diagram of the structure of the utility model without a cover. Fig. 3 is a schematic diagram of the front view structure of the utility model. Fig. 4 is a schematic diagram of the sectional structure along the A-A direction in Fig. 3 of the present invention. Fig. 5 is a partial enlarged structural schematic diagram of B in Fig. 4 of the present utility model.

The scheme of this embodiment provides a test tube for efficient separation of cell and tissue fluid, including a tube body 1, a cover body 2, a liquid outlet mechanism 3, a pressure relief mechanism and a suction tube 4. The inside of the tube body 1 has a tube body 1 divided into The partition 103 of the first cavity 101 and the second cavity 102, the section of the partition 103 is "L" shape, so that the bottom of the pipe body 1 forms a larger space, specifically as shown in Figure 4; The cover body 2 is covered on the opening of the tube body 1. It should be noted here that the cover body 2 is provided with a sealing sheet on the side facing the test tube, and the sealing sheet is used to prevent the first cavity 101 and the second cavity The body 102 leaks air or liquid from each other; the liquid outlet mechanism 3 is arranged on the partition 103, specifically the liquid outlet mechanism 3 is arranged on the transverse partition of the partition 103, and the first cavity 101 It communicates with the second cavity 102 through the liquid outlet mechanism 3; the pressure relief mechanism is arranged on the upper part of the cover body 2; one end of the suction pipe 4 is connected with the liquid outlet mechanism 3, and the suction pipe The other end of 4 is arranged at the bottom of the pipe body 1, the suction pipe 4 is arranged close to the side wall of the pipe body 1, the suction pipe 4 is integrally arranged with the bottom of the pipe body 1, and the suction pipe 4 The tonight port is set at the center of the bottom of the pipe body 1, and can completely absorb the deposition liquid at the bottom during use; the liquid outlet mechanism 3 includes: a liquid outlet hole 301, a fixing frame 302, a valve plate 304 and a spring 303, the liquid outlet hole 301 is provided with a movable chamber 306; the fixed frame 302 is arranged at the upper part of the movable chamber 306; the valve plate 304 resists the liquid outlet hole 301 and faces the second cavity 102 opening; the spring 303 is arranged between the fixed frame 302 and the valve plate 304;

In this embodiment, the specific use process is to inject the blood that needs to be separated into the second cavity. After centrifugation, the blood is separated into layers, the upper part is the supernatant, and the lower part is the sedimentation liquid. Connect with the first perforation to pump air, at this moment, the air pressure in the second cavity becomes smaller, and the valve plate 304 against the liquid outlet hole 301 opens, and the deposition liquid enters the second cavity through the liquid outlet hole 301, and deposits When the liquid enters the second cavity, the air inlet hole is opened to balance the air pressure in the second cavity. After the first cavity sucks the deposition liquid through the suction tube 4, when the supernatant liquid completely covers the bottom of the test tube, stop Aspirate, the valve plate 304 is pressed against the liquid outlet hole 301 again under the action of the spring 303, thereby completing the separation of tissue fluid; in this process, the supernatant liquid and the deposition liquid can be separated more thoroughly, reducing the contact with the outside world, and preventing contact with the tissue fluid. The interior of the test tube is protected from contamination.

As a preferred embodiment, the diameter of the active chamber 306 is larger than the diameter of the liquid outlet hole 301, the diameter of the valve plate 304 is larger than the diameter of the liquid outlet hole 301 and smaller than the diameter of the active chamber 306, in the valve plate Diversion grooves 305 are provided on both sides of 304 to facilitate deposition liquid entering the first cavity when the valve plate 304 is opened. It should be noted that the above structure is a simple one-way valve.

As a preferred embodiment, the pressure relief mechanism includes: an air suction port 201 and an air inlet 202, the air suction port 201 is facing the first cavity 101, a soft plug is arranged in the air suction port 201, The structure of the soft plug is a prior art and will not be described here. The soft plug is made of rubber plug or silicone material, and is pumped through a syringe or a small pump when in use; the air inlet 202 is facing the first The second cavity 102, the air inlet 202 is provided with a one-way air intake valve, when the deposition liquid enters the first cavity 101, the internal pressure of the second cavity 102 becomes smaller, the external pressure becomes larger, and the one-way air intake The valve is opened to inhale air to keep the pressure balance in the second cavity 102 .

The working principle of the utility model:

The utility model divides the test tube into the first cavity and the second cavity, injects the blood to be separated into the second cavity, and after centrifugation, the blood is layered, the upper part is the supernatant liquid, and the lower part is the sedimentary liquid. The needle tube is connected to the first perforation for pumping air. At this time, the air pressure in the second cavity becomes smaller, and the valve plate 304 against the liquid outlet hole 301 is opened, and the deposition liquid enters the second cavity through the liquid outlet hole 301. When the deposition liquid enters the second cavity, the air intake hole is opened to balance the air pressure in the second cavity. When the supernatant liquid completely fills the bottom of the tube body, the pumping is stopped to complete the separation of the interstitial fluid. The medium can make the supernatant and sedimentation liquid separate more thoroughly, reduce the contact with the outside world, and prevent the inside of the test tube from being polluted.

Finally, it should be noted that: the above is only a preferred embodiment of the utility model, and is not intended to limit the utility model, although the utility model has been described in detail with reference to the foregoing embodiments, for those skilled in the art , it can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present utility model, All should be included within the protection scope of the present utility model.

Claims (7)

1. A high efficiency separation tube for tissue fluid, comprising:

the pipe body (1), the inside of the pipe body (1) is provided with a partition (103) for dividing the pipe body (1) into a first cavity (101) and a second cavity (102);

the cover body (2) is arranged on the opening of the pipe body (1) in a covering manner;

the liquid outlet mechanism (3), the liquid outlet mechanism (3) is arranged on the partition (103), and the first cavity (101) and the second cavity (102) are communicated through the liquid outlet mechanism (3);

the pressure release mechanism is arranged at the upper part of the cover body (2);

and one end of the suction pipe (4) is connected with the liquid outlet mechanism (3), and the other end of the suction pipe (4) is arranged at the bottom of the pipe body (1).

2. The high-efficiency separation test tube for cell tissue fluid according to claim 1, wherein the cross section of the partition (103) is L-shaped.

3. The high-efficiency separation test tube for cell tissue fluid according to claim 1, wherein the fluid outlet mechanism (3) is arranged on a transverse partition plate of the partition (103), and the fluid outlet mechanism (3) comprises:

a liquid outlet hole (301), wherein a movable cavity (306) is arranged in the liquid outlet hole (301);

a fixed frame (302), wherein the fixed frame (302) is arranged at the upper part in the movable cavity (306);

a valve plate (304), wherein the valve plate (304) is propped against the liquid outlet hole (301) and faces to the opening of the second cavity (102);

-a spring (303), said spring (303) being arranged between said fixed frame (302) and said valve plate (304).

4. A tube for efficient separation of tissue fluid according to claim 3, characterized in that the diameter of said movable chamber (306) is larger than the diameter of said outlet opening (301).

5. A high efficiency separation test tube for cellular tissue fluid according to claim 1, characterized in that said suction tube (4) is arranged in close proximity to the side wall of said tube body (1).

6. The high efficiency separation tube of cellular tissue fluid of claim 1, wherein the pressure relief mechanism comprises:

the air extraction opening (201) is opposite to the first cavity (101), and a soft plug is arranged in the air extraction opening (201);

the air inlet (202) is opposite to the second cavity (102), and a one-way air inlet valve is arranged in the air inlet (202).

7. The high efficiency separation tube of cellular tissue fluid of claim 6 wherein the flexible plug is a rubber plug.