CN110973148A - Application of pyraclostrobin in preventing and treating ampullaria gigas - Google Patents
Application of pyraclostrobin in preventing and treating ampullaria gigas Download PDFInfo
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- CN110973148A CN110973148A CN201911315748.XA CN201911315748A CN110973148A CN 110973148 A CN110973148 A CN 110973148A CN 201911315748 A CN201911315748 A CN 201911315748A CN 110973148 A CN110973148 A CN 110973148A
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- pyraclostrobin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- General Health & Medical Sciences (AREA)
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- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
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- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
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Abstract
The invention provides application of pyraclostrobin in prevention and treatment of ampullaria gigas, belongs to the technical field of ampullaria gigas prevention and treatment, and relates to LC (liquid chromatography) of pyraclostrobin on ampullaria gigas50(48h)、LC50(72h) And LC50(96h) Respectively 0.463mg/L, 0.422mg/L and 0.402mg/L, which are equivalent to the molluscicidal effect of the molluscicidal amine ethanolamine salt. The pyraclostrobin provided by the invention can be used for preventing and treating ampullaria gigas while being used as a bactericide.
Description
Technical Field
The invention belongs to the technical field of ampullaria gigas prevention and treatment, and particularly relates to application of pyraclostrobin in prevention and treatment of ampullaria gigas.
Background
Pomacea canaliculata (Pomacea canaliculata) is a freshwater snail, namely apple snail, big bottle snail and snow snail, belonging to the phylum mollusca, the subclass gill and the family Pomacea. The ampullaria gigas is common in ingestion and large in food consumption, and particularly seriously harms rice, such as staple food of rice, cane shoots, taros and the like. The ampullaria gigas has strong adaptability and fertility, can be quickly dispersed into wetland and other natural fresh water environments from an agricultural region, seriously threatens the normal growth of the invasive field snails and aquatic plants, and is easy to destroy the integrity of the existing ecological system due to the invasion of the ampullaria gigas. The ampullaria gigas is originally produced in the amazon river basin, invades all over the world at present and is listed as one of hundreds of invasive species in the world. The ampullaria gigas are widely distributed in a wide area in the south of the Yangtze river in China, and in 2003, the ampullaria gigas are listed in a list of 16 'most harmful foreign species' which invade China. In addition, the ampullaria gigas is also an intermediate host of fasciolopsis mandshurica and angiostrongylus cantonensis, and has potential threat to human health.
At present, the prevention and treatment measures of ampullaria gigas comprise physical prevention and treatment, agricultural prevention and treatment, biological prevention and treatment and chemical prevention and treatment, but the chemical prevention and treatment still remains a main means for preventing and treating ampullaria gigas due to good prevention and treatment effect, quick response and low cost. 69 pesticide products registered in China for preventing and treating ampullaria gigas comprise niclosamide ethanolamine salt (33), niclosamide (25), metaldehyde (10) and calcium cyanamide (1) as effective components. The current registered pesticides for preventing and treating ampullaria gigas have the problems of few varieties, few formulations, repeated registration, serious product homogenization and the like. Therefore, the research and development of new medicaments for preventing and treating ampullaria gigas have important significance for controlling the diffusion of the ampullaria gigas and preventing the agricultural economy and the ecosystem of China from being damaged.
Disclosure of Invention
In view of the above, the invention aims to provide the application of pyraclostrobin in preventing and treating ampullaria gigas; LC of pyraclostrobin on ampullaria gigas50(48h)、LC50(72h) And LC50(96h) Respectively 0.463mg/L, 0.422mg/L and 0.402mg/L, which are equivalent to the molluscicidal effect of the molluscicidal amine ethanolamine salt. The pyraclostrobin provided by the invention can be used for preventing and treating ampullaria gigas while being used as a bactericide.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides application of pyraclostrobin in preventing and treating ampullaria gigas.
The invention also provides application of a preparation containing the pyraclostrobin in preventing and treating ampullaria gigas.
Preferably, the preparation formulation of the pyraclostrobin comprises a dispersible agent, wettable powder, missible oil, water dispersible granules, an aqueous emulsion, a microemulsion and a suspending agent.
Preferably, the LC of pyraclostrobin on ampullaria gigas50(48h)、LC50(72h) And LC50(96h) 0.463mg/L, 0.422mg/L and 0.402mg/L, respectively.
Preferably, the application is in particular to the application of the pyraclostrobin in paddy fields.
Preferably, the application amount of the pyraclostrobin is 20-40 g a.i./mu.
Preferably, the application amount of the pyraclostrobin is 25-35 g of a.i./mu.
Preferably, the application is carried out by spraying.
Preferably, the pyraclostrobin is diluted by 200-500 times and then is sprayed.
The invention has the beneficial effects that: the pyraclostrobin provided by the invention is applied to prevention and treatment of ampullaria gigas; LC of pyraclostrobin on ampullaria gigas50(48h)、LC50(72h) And LC50(96h) Respectively 0.463mg/L, 0.422mg/L and 0.402mg/L, which are equivalent to the molluscicidal effect of the molluscicidal amine ethanolamine salt. The pyraclostrobin provided by the invention can be used for preventing and treating ampullaria gigas while being used as a bactericide.
Detailed Description
Pyraclostrobin is a methoxy acrylate broad-spectrum bactericide with a pyrazole structure, is a mitochondrial respiration inhibitor, has the functions of protection, treatment and leaf penetration and conduction, and is registered and used on more than 150 crops in more than 60 countries around the world; in addition, pyraclostrobin is also a plant health activator, and is the first product registered for "plant health effects" by the U.S. environmental protection agency, the european union, and china.
The invention provides application of pyraclostrobin in preventing and treating ampullaria gigas.In the invention, the LC of pyraclostrobin on ampullaria gigas50(48h)、LC50(72h) And LC50(96h) Respectively 0.463mg/L, 0.422mg/L and 0.402mg/L, which are equivalent to the molluscicidal effect of the molluscicidal amine ethanolamine salt; can be used for preventing and treating ampullaria gigas.
The invention also provides application of a preparation containing the pyraclostrobin in preventing and treating ampullaria gigas. The invention has no special limitation on the dosage form, the effective component content and the like of the pyraclostrobin preparation, and the preparation form can be prepared by adopting the conventional pyraclostrobin in the field. In the specific implementation process of the invention, the preparation form of the kresoxim-methyl oxazole preparation takes missible oil as an example.
In the invention, the application is to apply the pyraclostrobin in a paddy field. The invention has no special limitation on the types of the crops, and all the crops which can be damaged by the ampullaria gigas can be selected, including the crops of rice, cane shoots, gorgon fruit and the like. In the specific implementation process of the invention, water bamboo is taken as an example. In the invention, the application amount of the pyraclostrobin is preferably 20-40 g/mu, more preferably 25-35 g/mu and most preferably 30 g/mu. In the present invention, the application is preferably performed by a spraying method; more preferably, the pyraclostrobin is diluted by 200-500 times and then is sprayed.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Snail killing activity determination of pyraclostrobin
The method is characterized by adopting a snail dipping method for determination. After being dissolved in methanol to fix volume, the pyraclostrobin original drug is prepared into a solution with the concentration of 2000mg/L, and then is diluted into a series of solutions with the concentrations (respectively 0.100mg/L, 0.150mg/L, 0.225mg/L, 0.338mg/L, 0.506mg/L, 0.759mg/L and 1.139mg/L) by aerated tap water.
Adding 2L of the above test solution into 2L beakers, placing 10 ampullaria gigas into each beaker, covering to prevent escape, culturing at room temperature (24 + -1 deg.C), observing death conditions of ampullaria gigas at 24, 48, 72, and 96 hr respectively, and testing during the test periodNo feeding is needed. The activity of the Chinese medicinal composition is observed by tapping to judge whether the Chinese medicinal composition is dead or not. The test was performed in 3 replicates and the mortality was averaged. Aerated tap water treatment was used as a blank control. By adopting a linear interpolation method, a curve of pyraclostrobin concentration to mortality rate several values is drawn, and LC (liquid chromatography) in 50% death is obtained50The value is obtained. The results are shown in Table 1.
TABLE 1 acute toxicity of pyraclostrobin bulk drug to ampullaria gigas
The results show that the mortality rate of ampullaria gigas in the blank control group is 0%; LC of pyraclostrobin on ampullaria gigas50(48h)、LC50(72h) And LC50(96h) 0.463mg/L, 0.422mg/L and 0.402mg/L, respectively. The result shows that the pyraclostrobin has good molluscicidal activity.
Example 2
Control effect of pyraclostrobin on ampullaria gigas
Materials and methods
Crops and targets
And (3) test crops: water bamboo; the control object is: ampullaria gigas
Reagent for testing
250g/L pyraclostrobin missible oil (BASF plant protection (Jiangsu) Co., Ltd.); 50% niclosamide ethanolamine salt wettable powder (Guangxi rural Biochemical Co., Ltd.)
Design of experiments
Let 3 treatments: 250g/L pyraclostrobin missible oil (the dosage of the preparation is 120 mL/mu); 50% niclosamide ethanolamine salt wettable powder (dosage of the preparation: 60 g/mu); clear water Control (CK). Each treatment was repeated 3 times, and the area of each cell was 30m2And small ridges are built among the communities to prevent water from flowing, and nets with the height of 60cm are used for enclosing the small ridges to prevent the ampullaria gigas from escaping.
Method of administering a drug
After the water bamboos are transplanted for 1 week, 30 ampullaria gigas (the body length is 2-3 cm) with similar size are bred in each district. Keeping a shallow water layer of 2-3cm in the field, and keeping the water for more than 7 days. The spraying method is adopted, and the pesticide is applied after being diluted by 30L of water per mu.
Investigation of drug efficacy
The number of live and dead snails was investigated at 2d and 7d after application, respectively, and the mortality and corrected mortality were calculated. Mortality and corrected mortality calculation:
mortality (%) < number of dead snails/(total number of snails-number of escape snails) × 100%;
control (%) corrected for (mortality of treatment group-mortality of control group)/(mortality of 100-control group) × 100%.
Analysis of results
According to the analysis of test results, the control effect of 250g/L pyraclostrobin missible oil treatment is 76.7% and the control effect of 50% niclosamide ethanolamine salt wettable powder treatment is 85.6% after 2d of pesticide application; at 7d after application, the control effect of 250g/L pyraclostrobin missible oil treatment is 85.4 percent, and the control effect of 50 percent niclosamide ethanolamine salt wettable powder treatment is 93.3 percent.
The test result shows that compared with 50% niclosamide ethanolamine salt wettable powder, 250g/L pyraclostrobin missible oil has a good control effect on ampullaria gigas, and is an ideal medicament for controlling ampullaria gigas in a water bamboo field. The pyraclostrobin missible oil can not be used in the pregnancy of the water bamboo.
Comparative example 1
Molluscicidal activity of niclosamide ethanolamine salts
The method is characterized by adopting a snail dipping method for determination. The niclosamide ethanolamine salt raw material is dissolved by methanol to fix volume to prepare a solution with the concentration of 2000mg/L, and then diluted by aerated tap water to prepare a series of solutions with the concentrations of 0.100mg/L, 0.130mg/L, 0.169mg/L, 0.220mg/L, 0.286mg/L, 0.371mg/L and 0.483mg/L respectively.
2L of the test solution with different concentrations are added into 2L beakers, 10 ampullaria gigas are placed into each beaker, the beakers are covered to prevent the ampullaria gigas from escaping, the ampullaria gigas are cultured at room temperature (24 +/-1 ℃), the death condition of the ampullaria gigas is observed at 24 hours, 48 hours, 72 hours and 96 hours respectively, and the ampullaria gigas are not fed during the test period. The activity of the Chinese medicinal composition is observed by tapping to judge whether the Chinese medicinal composition is dead or not. The test was performed in 3 replicates and the mortality was averaged. Aerated tap water treatment was used as a blank control. Adopting a linear interpolation method to draw the concentration of niclosamide ethanolamine saltDegree vs. mortality several value curve, and LC at 50% death was determined50The value is obtained. The results are shown in Table 2.
TABLE 2 acute toxicity of niclosamide ethanolamine salt bulk drug to ampullaria gigas
The results show that the mortality rate of ampullaria gigas in the blank control group is 0%; LC of niclosamide ethanolamine salt on ampullaria gigas50(24h)、LC50(48h)、LC50(72h) And LC50(96h) 0.347, 0.315, 0.297 and 0.324mg/L, respectively.
The results of the above embodiments show that the pyraclostrobin provided by the invention has equivalent molluscicidal effect on ampullaria gigas and molluscicidal amine ethanolamine salt, and can be used for preventing and treating ampullaria gigas. The pyraclostrobin provided by the invention has broad-spectrum bactericidal effect, and can be used as a bactericide and an ampullaria gigas control agent.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. Application of pyraclostrobin in preventing and treating ampullaria gigas is provided.
2. Use of a formulation comprising pyraclostrobin as claimed in claim 1 for the control of ampullaria gigas.
3. The use according to claim 2, wherein the formulation of pyraclostrobin comprises dispersible agents, wettable powders, emulsifiable concentrates, water dispersible granules, aqueous emulsions, microemulsions and suspending agents.
4. The use according to claim 1, wherein the LC of pyraclostrobin on pomacea canaliculata50(48h)、LC50(72h) And LC50(96h) 0.463mg/L, 0.422mg/L and 0.402mg/L, respectively.
5. The use according to claim 1, characterized in that the use is in particular the application of pyraclostrobin in a paddy field.
6. The use according to claim 5, wherein pyraclostrobin is applied in an amount of 20-40 ga.i./acre.
7. The use according to claim 6, wherein the pyraclostrobin is applied in an amount of 25 to 35 ga.i./acre.
8. Use according to claim 5, characterized in that the application is carried out by spraying.
9. The use according to claim 8, wherein the pyraclostrobin is diluted 200-to 500-fold and then applied by spraying.
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| CN201911315748.XA CN110973148A (en) | 2019-12-19 | 2019-12-19 | Application of pyraclostrobin in preventing and treating ampullaria gigas |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101951772A (en) * | 2008-01-29 | 2011-01-19 | 先正达参股股份有限公司 | Molluscicide |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101951772A (en) * | 2008-01-29 | 2011-01-19 | 先正达参股股份有限公司 | Molluscicide |
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Application publication date: 20200410 |