CN110938540A

CN110938540A - Enzymolysis device is used in polypeptide production

Info

Publication number: CN110938540A
Application number: CN201911229422.5A
Authority: CN
Inventors: 谢双
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-12-04
Filing date: 2019-12-04
Publication date: 2020-03-31

Abstract

The invention discloses an enzymolysis device for polypeptide production, which belongs to the technical field of bioengineering and comprises a raw material crushing mechanism, a multi-stage grinding mechanism, a homogenate mixing mechanism, a grinding box, a packing auger feeding machine, a filtering and drying mechanism and an enzymolysis tank, wherein the top of the grinding box is provided with the crushing box, the top of the grinding box is provided with a guide hopper, the guide hopper is provided with a screening hole, the side of the crushing box is provided with a large-particle secondary crushing component, the multi-stage grinding mechanism comprises a grinding shell, a multi-stage grinding block and a rotating component, and the bottom of the grinding shell is provided with a grinding chamber. The invention has the advantages that the raw material can be automatically crushed by the raw material crushing mechanism, raw material particles which are not completely crushed can be conveyed into the crushing box again under the action of the large-particle secondary crushing assembly, the vibration of the drying basin can be realized by the filtering and drying mechanism, and the polypeptide concentrated solution in the drying basin can be spray-dried under the action of the spray drying of the spray nozzle.

Description

Enzymolysis device is used in polypeptide production

Technical Field

The invention relates to the technical field of biological engineering, in particular to an enzymolysis device for polypeptide production.

Background

The enzymatic hydrolysis of animal and plant proteins is a process of hydrolyzing animal and plant proteins at a certain temperature under the action of enzyme. The traditional enzymolysis process of animal and plant protein mostly adopts intermittent operation, namely, one-time feeding, enzyme adding, hydrolysis, separation, enzyme inactivation and the like, and the enzyme method polypeptide is polypeptide obtained by degrading protein with protease. The enzyme method for producing polypeptide represents the level and trend of polypeptide research, development, production and innovation in the world today.

The invention with the publication number of CN107354091A discloses a soybean polypeptide enzymolysis device, which comprises an enzymolysis tank body; a stirring device is arranged in the center of the interior of the enzymolysis tank body, a feed inlet is arranged on one side of the top of the enzymolysis tank body, and a discharge outlet is arranged in the center of the bottom of the enzymolysis tank body; the stirring device comprises a rotating shaft and stirring blades spirally arranged by taking the rotating shaft as a center, and one end of the stirring device is connected with the compressed air tank through an air pipe and is driven to rotate through a power device; the ultrasonic generator is annularly arranged in the center of the inner wall of the tank body by taking the center of the enzymolysis tank body as a circle center. The method is simple to operate, can fully and uniformly mix the materials, and improves the enzymolysis reaction efficiency; the enzymolysis of the soybean polypeptide is effectively promoted by using ultrasonic waves, and the enzymolysis effect is ensured.

However, the above apparatus still has the following problems: firstly, raw materials for preparing biological polypeptide are various, most of the raw materials need to be crushed to facilitate later enzymolysis operation, so that the extraction rate of the biological polypeptide is integrally improved, and although the device can fully and uniformly mix materials, the device cannot finish multi-stage grinding of the materials, and cannot meet the requirements of different users; secondly, the mixture after enzymolysis cannot be effectively spray-dried to obtain the required biological polypeptide powder, so that the mixture after enzymolysis needs to be manually subjected to subsequent drying by workers, and great troubles are brought to the workers.

Disclosure of Invention

The invention aims to solve the technical problem of providing an enzymolysis device for polypeptide production, which aims to solve the technical problems that multi-stage grinding of materials cannot be completed and effective spray drying of a mixture after enzymolysis cannot be realized.

In order to solve the technical problems, the invention provides the following technical scheme: comprises a raw material crushing mechanism, a multi-stage grinding mechanism, a homogenate mixing mechanism, a grinding box, an auger feeding machine, a filtering and drying mechanism and an enzymolysis tank, wherein the filtering and drying mechanism is arranged below the enzymolysis tank, the top of the grinding box is provided with the crushing box communicated with the inside of the grinding box, the raw material crushing mechanism and the multi-stage grinding mechanism are respectively arranged inside the crushing box and the grinding box, the homogenate mixing mechanism is arranged below the inside of the grinding box, the auger feeding machine is arranged between the grinding box and the enzymolysis tank, the top of the grinding box is provided with a guide hopper which is obliquely arranged, the guide hopper is provided with a screening hole, the side of the grinding box is provided with a large-particle secondary crushing component, the multi-stage grinding mechanism comprises a grinding shell, a multi-stage grinding block and a rotating component for driving the multi-stage grinding block to rotate, the bottom of the grinding shell is provided with a grinding chamber, the multistage grinding block is arranged below the grinding shell.

Further, multistage grinding piece is including last grinding piece, well grinding piece and the lower grinding piece that sets gradually along vertical direction, the diameter of going up grinding piece, well grinding piece and lower grinding piece increases progressively along vertical direction in proper order, the top of going up grinding piece is equipped with the guide inclined plane, grind the room including last grinding region, well grinding region and lower grinding region, it cooperatees with last grinding piece to go up grinding region, well grinding region and well grinding piece cooperate, lower grinding region cooperatees with lower grinding piece, it is the round platform structure to go up grinding piece, well grinding piece and lower grinding piece, the diameter of grinding piece upper surface in the diameter of going up grinding piece lower surface, the diameter of well grinding piece lower surface is less than the diameter of grinding piece upper surface down.

Further, the runner assembly sets up the lift cylinder at the mount frame top including the lifting disk that the level set up, setting at the inside mounting bracket of grinding case and two intervals, the top of mounting bracket is equipped with the column spinner of vertical setting, the top of column spinner and the bottom fixed connection who grinds the piece down, the bottom of column spinner passes through the bearing with the top of lifting disk and is connected, the bottom of lifting disk is equipped with step motor, the cover is equipped with the driving gear on step motor's the output, the cover is equipped with the driven gear with driving gear engagement on the column spinner, the top of lifting disk is equipped with the spacing ring, the bottom of grinding the piece down is equipped with three and spacing ring sliding fit's support slide bar.

Further, smash the top of case and be equipped with the rotating electrical machines, prevent the funnel and set up the support frame at crushing incasement portion, the rotating electrical machines sets up on the lateral wall of smashing the case, the top of support frame is equipped with rather than normal running fit's rotation post, the cover is equipped with the crisscross crushing blade that sets up of a plurality of on the rotation post, the cover is equipped with driving pulley on the output of rotating electrical machines, the bottom cover that rotates the post is equipped with driven pulley, be connected through belt transmission between driving pulley and the driven pulley.

Further, large granule secondary crushing subassembly is including conveying motor, a transport section of thick bamboo and the vertical transport axle that sets up in a transport section of thick bamboo, it is equipped with helical blade to carry epaxial cover, conveying motor sets up the top at a transport section of thick bamboo, conveying motor's output and the top fixed connection who carries the axle, the top of a transport section of thick bamboo and the inside of smashing the case are equipped with feed channel, the inside bottom of smashing the case be equipped with the inside discharge passageway who communicates of a transport section of thick bamboo.

Further, homogenate mixing mechanism is including agitator motor, the play hopper that is the slope and sets up the (mixing) shaft at grinding the case hypomere, agitator motor sets up on grinding the lateral wall of case, agitator motor's output and the tip fixed connection of (mixing) shaft, the cover is equipped with a plurality of stirring paddle leaf on the (mixing) shaft, the both sides of grinding the case all are equipped with sterilamp.

Further, it is including uncovered ascending dry basin and the three dead lever that is the circumference and distributes in dry basin below to filter dry mechanism, the top of dead lever is equipped with rather than sliding fit's slide bar, the top of slide bar and the bottom fixed connection of dry basin, the cover is equipped with the vibrations spring on the slide bar, the both ends of vibrations spring respectively with the top of dead lever and the bottom fixed connection of dry basin, the below of dry basin is equipped with the vibrations cylinder of vertical setting, be equipped with the vibrations dish on the output of vibrations cylinder, the side of dry basin is equipped with the support, the support top is equipped with dry pipeline, the last fog nozzle that is equipped with of dry pipeline.

Further, the top of enzymolysis tank is equipped with the mixing motor with enzymolysis tank lateral wall fixed connection and with the charge-in pipeline of auger material loading machine output intercommunication, be equipped with the conveying pipeline rather than inside intercommunication on the enzymolysis tank, the inside of enzymolysis tank is equipped with the link that two levels set up, two be equipped with between the link with both normal running fit's axis of rotation, the cover is equipped with the mixing paddle in the axis of rotation, the cover is equipped with first belt pulley on the output of mixing motor, the top cover of axis of rotation is equipped with the second belt pulley, be connected through belt transmission between first belt pulley and the second belt pulley, the inside bottom of enzymolysis tank is equipped with the molecular membrane filter, the lateral wall of enzymolysis tank is equipped with the heater.

Compared with the prior art, the invention has the beneficial effects that:

firstly, the invention is provided with a raw material crushing mechanism and a multi-stage grinding mechanism, the raw material can be automatically crushed by the raw material crushing mechanism, so that the raw material can be crushed into required particles, and the crushed raw material particles can enter the grinding box through the screening holes, the raw material particles which are not completely crushed can be conveyed into the crushing box again under the action of the large particle secondary crushing component to crush the raw material into material particles with a certain size, can grind the powder process once more to the material granule after smashing automatically through multistage grinding mechanism, make the material granule can reach the required particle size of user, be convenient for grind the biological raw materials of different grade type, not only make things convenient for the enzymolysis operation in later stage, can also wholly improve biological polypeptide's extraction rate to can not accomplish among the solution prior art and carry out multistage grinding's technical problem to the material.

Secondly, the biological polypeptide powder preparation device is provided with a filtering and drying mechanism, vibration of a drying basin can be realized through the filtering and drying mechanism, polypeptide concentrated solution in the drying basin can be vibrated, the polypeptide concentrated solution is heated uniformly, a spray nozzle can blow hot air, and the polypeptide concentrated solution in the drying basin can be spray-dried under the action of spray drying of the spray nozzle to obtain biological polypeptide powder; the technical problem that the mixture after enzymolysis cannot be effectively spray-dried in the prior art is solved.

Thirdly, the grinding box is provided with a homogenizing and mixing mechanism, ground raw material powder and solution can be fully homogenized and mixed through the homogenizing and mixing mechanism, and the ultraviolet sterilizing lamps on the two sides of the grinding box can sterilize biological homogenate.

The biological homogenate liquid can be conveyed to the interior of the enzymolysis tank under the action of the auger feeding machine, can be subjected to heat preservation and enzymolysis in the enzymolysis tank, and can be filtered under the action of the molecular membrane filter to obtain the polypeptide concentrated solution.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic top view of the present invention;

FIG. 3 is a cross-sectional view taken at A-A of FIG. 2;

FIG. 4 is a partial cross-sectional view of the first embodiment of the present invention;

FIG. 5 is a partial sectional view of the second embodiment of the present invention;

FIG. 6 is an enlarged view of FIG. 5 at B;

FIG. 7 is a schematic perspective view of the filter-drying mechanism and the enzymolysis tank;

fig. 8 is a partial cross-sectional view of the third embodiment of the present invention.

The reference numbers in the figures are:

the raw material crushing mechanism 1, the multistage grinding mechanism 2, the grinding shell 21, the grinding chamber 22, the multistage grinding block 23, the upper grinding block 24, the material guide slope 241, the middle grinding block 25, the lower grinding block 26, the upper grinding area 27, the middle grinding area 271, the lower grinding area 272, the rotating component 28, the lifting disc 281, the mounting rack 282, the lifting cylinder 283, the rotating column 284, the stepping motor 285, the driving gear 286, the driven gear 287, the limiting ring 29, the supporting slide bar 291, the homogenizing and mixing mechanism 3, the stirring motor 31, the discharging hopper 32, the stirring shaft 33, the stirring paddle 34, the ultraviolet sterilizing lamp 35, the grinding box 4, the auger feeder 5, the filtering and drying mechanism 6, the drying basin 61, the fixing rod 62, the sliding rod 63, the vibrating spring 64, the vibrating cylinder 65, the vibrating disc 66, the bracket 67, the drying pipeline 68, the spray nozzle 69, the enzymolysis tank 7, the blending motor 71, the feeding pipeline 72 and the material conveying pipeline 73, link 74, axis of rotation 75, first belt pulley 76, mixing paddle 77, second belt pulley 78, molecular membrane filter 79 smashes case 8, rotating electrical machines 81, leak protection fill 82, support frame 83, rotation post 84, smash blade 85, drive pulley 86, driven pulley 87, guide hopper 88, screening hole 881, large granule regrinding subassembly 9, conveying motor 91, transport cylinder 92, carry axle 93, helical blade 94, charge-in pipeline 95, discharge passage 96.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

Referring to fig. 1 to 8, the device comprises a raw material crushing mechanism 1, a multi-stage grinding mechanism 2, a homogenate mixing mechanism 3, a grinding box 4, an auger feeding machine 5, a filtering and drying mechanism 6 and an enzymolysis tank 7, wherein the filtering and drying mechanism 6 is arranged below the enzymolysis tank 7, the top of the grinding box 4 is provided with a crushing box 8 communicated with the inside of the grinding box 4, the raw material crushing mechanism 1 and the multi-stage grinding mechanism 2 are respectively arranged inside the crushing box 8 and the grinding box 4, the homogenate mixing mechanism 3 is arranged below the inside of the grinding box 4, the auger feeding machine 5 is arranged between the grinding box 4 and the enzymolysis tank 7, the top of the crushing box 8 is provided with a guide hopper 88 arranged obliquely, the guide hopper 88 is provided with a screening hole 881, the side of the crushing box 8 is provided with a large-particle secondary crushing assembly 9, the multi-stage grinding mechanism 2 comprises a grinding shell 21, A multi-stage grinding block 23 and a rotating assembly 28 for driving the multi-stage grinding block 23 to rotate, wherein the grinding chamber 22 is arranged at the bottom of the grinding shell 21, and the multi-stage grinding block 23 is arranged below the grinding shell 21. The raw material can be automatically crushed by the raw material crushing mechanism 1, so that the raw material can be crushed into required particles, the crushed raw material particles can enter the grinding box 4 through the screening hole 881, the raw material particles which are not completely crushed can be conveyed into the grinding box 8 again under the action of the large particle secondary crushing component 9 to crush the raw material into material particles with certain size, the crushed material particles can be automatically ground again to prepare powder by the multistage grinding mechanism 2, the material particles can reach the particle size required by a user, the biological raw materials of different types can be conveniently ground, the later enzymolysis operation is facilitated, the extraction rate of biological polypeptide can be integrally improved, the ground raw material powder and solution can be fully homogenized and mixed by the homogenizing and mixing mechanism 3, and the sterilization operation on the biological uniform slurry can be realized by the ultraviolet germicidal lamps 35 at the two sides of the grinding box 4, the vibration of the drying basin 61 can be realized through the filtering and drying mechanism 6, so that the polypeptide concentrated solution in the drying basin 61 can vibrate, the spray nozzle 69 can blow hot air, and the polypeptide concentrated solution in the drying basin 61 can be spray-dried under the spray drying effect of the spray nozzle 69 to obtain biological polypeptide powder.

The multistage grinding block 23 comprises an upper grinding block 24, a middle grinding block 25 and a lower grinding block 26 which are sequentially arranged along the vertical direction, the diameters of the upper grinding block 24, the middle grinding block 25 and the lower grinding block 26 are sequentially increased along the vertical direction, a material guide inclined plane 241 is arranged at the top of the upper grinding block 24, the grinding chamber 22 comprises an upper grinding area 27, a middle grinding area 271 and a lower grinding area 272, the upper grinding area 27 is matched with the upper grinding block 24, the middle grinding area 271 is matched with the middle grinding block 25, the lower grinding area 272 is matched with the lower grinding block 26, the upper grinding block 24, the middle grinding block 25 and the lower grinding block 26 are all in a circular truncated cone structure, the diameter of the upper surface of the grinding block 25 in the diameter of the lower surface of the upper grinding block 24, and the diameter of the lower surface of the middle grinding block 25 is smaller than the diameter of the upper surface of the lower grinding block 26; can grind the powder process once more automatically to the material granule after smashing through multistage grinding mechanism 2, make the material granule can reach the required particle size of user, be convenient for grind the biological raw materials of different grade type, not only make things convenient for the enzymolysis operation in later stage, can also wholly improve biological polypeptide's extraction rate, lift cylinder 283 work can drive lifter plate 281 and go up and down, make lifter plate 281 can drive abrasive brick 24, well abrasive brick 25 and the whole upward movement of lower abrasive brick 26, make the worker can be to the distance between last abrasive brick 24 and the last grinding region 27, the distance between well abrasive brick 25 and the well grinding region 271 and the distance between lower abrasive brick 26 and the lower grinding region 272 are adjusted, the worker of being convenient for can produce the raw materials of required particle size.

The rotating assembly 28 comprises a horizontally arranged lifting disc 281, an installation frame 282 arranged inside the grinding box 4 and two lifting cylinders 283 arranged at the top of the installation frame 282 at intervals, wherein a vertically arranged rotating column 284 is arranged at the top of the installation frame 282, the top end of the rotating column 284 is fixedly connected with the bottom of the lower grinding block 26, the bottom end of the rotating column 284 is connected with the top end of the lifting disc 281 through a bearing, a stepping motor 285 is arranged at the bottom of the lifting disc 281, a driving gear 286 is sleeved on the output end of the stepping motor 285, a driven gear 287 meshed with the driving gear 286 is sleeved on the rotating column 284, a limit ring 29 is arranged at the top of the lifting disc 281, and three support sliding rods 291 slidably matched with the limit ring 29 are arranged at the bottom of the lower grinding block 26; stepping motor 285 can drive and can drive driving gear 286 and rotate, make driving gear 286 can drive rather than the driven gear 287 of meshing and take place to rotate, realize the rotation of column spinner 284, make column spinner 284 can drive down grind piece 26 and take place to rotate, make and go up grind piece 24, well grind piece 25 and grind piece 26 and rotate simultaneously down, come to carry out multistage grinding operation to the raw materials granule, support slide bar 291 can with spacing ring 29 sliding fit, make down grind piece 26 can rotate along the axis of column spinner 284, make and go up grind piece 24, well grind piece 25 and grind piece 26 and rotate simultaneously down, come to carry out multistage grinding operation to the raw materials granule.

The top of the crushing box 8 is provided with a rotating motor 81, a leakage-proof hopper 82 and a support frame 83 arranged inside the crushing box 8, the rotating motor 81 is arranged on the side wall of the crushing box 8, the top of the support frame 83 is provided with a rotating column 84 in rotating fit with the support frame, a plurality of crushing blades 85 arranged in a staggered mode are sleeved on the rotating column 84, a driving belt pulley 86 is sleeved on the output end of the rotating motor 81, a driven belt pulley 87 is sleeved at the bottom end of the rotating column 84, and the driving belt pulley 86 is in transmission connection with the driven belt pulley 87 through a belt; rotating electrical machines 81 work can drive belt pulley 86 and take place to rotate, make drive belt pulley 86 drive driven pulley 87 through the belt and take place to rotate, make rotation post 84 can drive the crisscross crushing blade 85 that sets up of a plurality of rotate, crushing blade 85 can carry out preliminary crushing to the material that gets into to crushing incasement 8 at the pivoted in-process, the material after smashing can get into to grinding chamber 22 in through the sieve mesh, the great material of granule can get into to discharge passage 96 under the guide fill 88 effect that the slope set up.

The large particle secondary crushing assembly 9 comprises a conveying motor 91, a conveying cylinder 92 and a conveying shaft 93 vertically arranged in the conveying cylinder 92, wherein a spiral blade 94 is sleeved on the conveying shaft 93, the conveying motor 91 is arranged at the top of the conveying cylinder 92, the output end of the conveying motor 91 is fixedly connected with the top end of the conveying shaft 93, a material guide pipeline 95 is arranged between the top of the conveying cylinder 92 and the inside of the crushing box 8, and a material discharge channel 96 communicated with the inside of the conveying cylinder 92 is arranged at the bottom end of the inside of the crushing box 8; large granule material can enter into to transport section of thick bamboo 92 in through discharge passage 96, and axle 93 takes place to rotate can be driven in the work of conveying motor 91, makes to carry axle 93 to drive helical blade 94 and takes place to rotate, makes helical blade 94 can drive the material that enters into to transport section of thick bamboo 92 and upwards carries, makes the material can carry to the inside of smashing case 8 through guide pipe 95, is convenient for smash blade 85 and smashes the operation once more to large granule material.

The homogenate mixing mechanism 3 comprises a stirring motor 31, a discharge hopper 32 arranged in an inclined manner and a stirring shaft 33 arranged at the lower section of the grinding box 4, wherein the stirring motor 31 is arranged on the side wall of the grinding box 4, the output end of the stirring motor 31 is fixedly connected with the end part of the stirring shaft 33, a plurality of stirring blades 34 are sleeved on the stirring shaft 33, and ultraviolet sterilizing lamps 35 are arranged on two sides of the grinding box 4; can realize carrying out abundant homogenate through homogenate mixing mechanism 3 and mixing to the former feed powder after grinding with solution to grind the sterilamp 35 of 4 both sides of case and can realize the operation of disinfecting to the equal thick liquid of organism, agitator motor 31 work can drive (mixing) shaft 33 and take place to rotate, makes (mixing) shaft 33 can drive stirring paddle leaf 34 and takes place to rotate, makes a plurality of stirring paddle leaf 34 can be to the abundant equal thick liquid of solution and former feed powder and mix.

The filtering and drying mechanism 6 comprises a drying basin 61 with an upward opening and three fixing rods 62 distributed circumferentially below the drying basin 61, a sliding rod 63 in sliding fit with the fixing rod 62 is arranged at the top of the fixing rod 62, the top end of the sliding rod 63 is fixedly connected with the bottom end of the drying basin 61, a vibration spring 64 is sleeved on the sliding rod 63, two ends of the vibration spring 64 are fixedly connected with the top end of the fixing rod 62 and the bottom of the drying basin 61 respectively, a vertically arranged vibration cylinder 65 is arranged below the drying basin 61, a vibration disc 66 is arranged at the output end of the vibration cylinder 65, a support 67 is arranged beside the drying basin 61, a drying pipeline 68 is arranged at the top of the support 67, and a spray nozzle 69 is arranged on the drying pipeline 68; the vibration of the drying basin 61 can be realized through the filtering and drying mechanism 6, so that the polypeptide concentrated solution in the drying basin 61 can vibrate, the spray nozzle 69 can blow hot air, and the polypeptide concentrated solution in the drying basin 61 can be spray-dried under the spray drying effect of the spray nozzle 69 to obtain biological polypeptide powder.

The top of the enzymolysis tank 7 is provided with a mixing motor 71 fixedly connected with the side wall of the enzymolysis tank 7 and a feeding pipeline 72 communicated with the output end of the auger feeding machine 5, the enzymolysis tank 7 is provided with a conveying pipeline 73 communicated with the inside of the enzymolysis tank 7, the inside of the enzymolysis tank 7 is provided with two horizontally arranged connecting frames 74, a rotating shaft 75 in running fit with the connecting frames 74 is arranged between the two connecting frames 74, the rotating shaft 75 is sleeved with a mixing paddle 77, the output end of the mixing motor 71 is sleeved with a first belt pulley 76, the top end of the rotating shaft 75 is sleeved with a second belt pulley 78, the first belt pulley 76 is connected with the second belt pulley 78 through belt transmission, the bottom end of the inside of the enzymolysis tank 7 is provided with a molecular membrane filter 79, and the side wall of the enzymolysis tank 7 is provided with a heater; can carry the inside of the equal thick liquid of biology to enzymolysis tank 7 under auger material loading machine 5's effect, the equal thick liquid can keep warm the enzymolysis in enzymolysis tank 7, and can filter the equal thick liquid under molecular film filter 79's effect, obtain the polypeptide concentrate, mixing motor 71 work can drive first belt pulley 76 and take place to rotate, make first belt pulley 76 can drive second belt pulley 78 through the belt and take place to rotate, make second belt pulley 78 can drive axis of rotation 75 and take place to rotate, make axis of rotation 75 can drive mixing paddle 77 and take place to rotate, make mixing paddle 77 can carry out the mixing to enzymolysis liquid and biological polypeptide mixed liquid, be convenient for carry out the enzymolysis operation to the polypeptide.

The working principle of the invention is as follows: the worker manually puts the raw material for preparing biological polypeptide into the crushing box 8, the raw material can be automatically crushed by the raw material crushing mechanism 1, the raw material can be crushed into required particles, the crushed raw material particles can enter the grinding box 4 through the screening hole 881, the incompletely crushed raw material particles can be conveyed into the crushing box 8 again under the action of the large particle secondary crushing component 9 to crush the raw material into material particles with certain size, the rotary motor 81 can drive the driving belt pulley 86 to rotate when working, the driving belt pulley 86 can drive the driven belt pulley 87 to rotate through a belt, so that the rotating column 84 rotates, the rotating column 84 can drive a plurality of crushing blades 85 which are arranged in a staggered mode to rotate, and the crushing blades 85 can primarily crush the material entering the crushing box 8 in the rotating process, the crushed material can enter the grinding chamber 22 through the sieve holes, the material with larger particles can enter the discharge passage 96 under the action of the guide hopper 88 arranged obliquely, so that the large-particle material can enter the conveying cylinder 92 through the discharge passage 96, the conveying motor 91 can drive the conveying shaft 93 to rotate when working, the conveying shaft 93 can drive the helical blade 94 to rotate, the helical blade 94 can drive the material entering the conveying cylinder 92 to be conveyed upwards, the material can be conveyed to the inside of the crushing box 8 through the guide pipeline 95, the crushing blade 85 can conveniently crush the large-particle material again, the crushed material particles can be automatically ground again through the multistage grinding mechanism 2 to prepare powder, the material particles can reach the particle size required by a user, the biological raw materials of different types can be conveniently ground, and the later enzymolysis operation is facilitated, the extraction rate of biological polypeptide can be integrally improved, the lifting cylinder 283 can drive the lifting disc 281 to lift, the lifting disc 281 can drive the upper grinding block 24, the middle grinding block 25 and the lower grinding block 26 to integrally move upwards, a worker can adjust the distance between the upper grinding block 24 and the upper grinding area 27, the distance between the middle grinding block 25 and the middle grinding area 271 and the distance between the lower grinding block 26 and the lower grinding area 272, the worker can conveniently manufacture raw materials with required particle size, the stepping motor 285 can drive the driving gear 286 to rotate, the driving gear 286 can drive the driven gear 287 engaged with the driving gear 286 to rotate so as to realize the rotation of the rotating column 284, the rotating column 284 can drive the lower grinding block 26 to rotate, so that the upper grinding block 24, the middle grinding block 25 and the lower grinding block 26 rotate simultaneously to carry out multi-stage grinding operation on the raw material particles, the ground raw material powder and the solution can be fully homogenized and mixed through the homogenizing and mixing mechanism 3, the ultraviolet sterilizing lamps 35 on the two sides of the grinding box 4 can sterilize the biological homogenate liquid, the stirring shaft 33 can be driven to rotate by the operation of the stirring motor 31, the stirring shaft 33 can drive the stirring blades 34 to rotate, so that the stirring blades 34 can fully homogenize and mix the solution and the raw material powder, the biological homogenate liquid can be conveyed to the inside of the enzymolysis tank 7 under the action of the auger feeder 5, the homogenate liquid can be subjected to heat preservation enzymolysis in the enzymolysis tank 7, the homogenate liquid can be filtered under the action of the molecular membrane filter 79, so that the polypeptide concentrated liquid can be obtained, the vibration of the drying basin 61 can be realized through the filtering and drying mechanism 6, the polypeptide concentrated liquid in the drying basin 61 can be vibrated, the spray nozzle 69 can blow hot air, the polypeptide concentrated solution in the drying basin 61 can be spray-dried under the action of the spray nozzle 69 to obtain biological polypeptide powder.

Claims

1. The enzymolysis device for polypeptide production is characterized by comprising a raw material crushing mechanism (1), a multi-stage grinding mechanism (2), a homogenate mixing mechanism (3), a grinding box (4), a packing auger feeding machine (5), a filtering and drying mechanism (6) and an enzymolysis tank (7), wherein the filtering and drying mechanism (6) is arranged below the enzymolysis tank (7), the top of the grinding box (4) is provided with a crushing box (8) communicated with the inside of the grinding box, the raw material crushing mechanism (1) and the multi-stage grinding mechanism (2) are respectively arranged inside the grinding box (8) and the grinding box (4), the homogenate mixing mechanism (3) is arranged below the inside of the grinding box (4), the packing auger feeding machine (5) is arranged between the grinding box (4) and the enzymolysis tank (7), the top of the grinding box (8) is provided with a guide hopper (88) which is obliquely arranged, be equipped with screening hole (881) on guide hopper (88), the side of smashing case (8) is equipped with large granule secondary crushing subassembly (9), multistage grinding mechanism (2) are including grinding shell (21), multistage grinding piece (23) and be used for driving multistage grinding piece (23) and take place rotatory runner assembly (28), the bottom of grinding shell (21) is equipped with grinds room (22), multistage grinding piece (23) set up the below at grinding shell (21).

2. The enzymolysis device for polypeptide production according to claim 1, wherein said multi-stage grinding block (23) comprises an upper grinding block (24), a middle grinding block (25) and a lower grinding block (26) which are sequentially arranged along a vertical direction, the diameters of said upper grinding block (24), said middle grinding block (25) and said lower grinding block (26) are sequentially increased along the vertical direction, the top of said upper grinding block (24) is provided with a material guiding bevel (241), said grinding chamber (22) comprises an upper grinding region (27), a middle grinding region (271) and a lower grinding region (272), said upper grinding region (27) and said upper grinding block (24) are matched, said middle grinding region (271) and said middle grinding block (25) are matched, said lower grinding region (272) and said lower grinding block (26) are matched, said upper grinding block (24), said middle grinding block (25) and said lower grinding block (26) are all in a circular truncated cone structure, the diameter of the lower surface of the upper grinding block (24) is equal to that of the upper surface of the grinding block (25), and the diameter of the lower surface of the middle grinding block (25) is smaller than that of the upper surface of the lower grinding block (26).

3. The enzymolysis device for polypeptide production according to claim 2, wherein said rotating assembly (28) comprises a horizontally disposed lifting plate (281), a mounting frame (282) disposed inside the grinding box (4), and two lifting cylinders (283) spaced apart from each other and disposed on the top of the mounting frame (282), the top of the mounting frame (282) is provided with a vertically disposed rotating column (284), the top of the rotating column (284) is fixedly connected with the bottom of the lower grinding block (26), the bottom of the rotating column (284) is connected with the top of the lifting plate (281) through a bearing, the bottom of the lifting plate (281) is provided with a stepping motor (285), the output end of the stepping motor (285) is sleeved with a driving gear (286), the rotating column (284) is sleeved with a driven gear (287) engaged with the driving gear (286), the top of the lifting plate (281) is provided with a limit ring (29), the bottom of the lower grinding block (26) is provided with three supporting slide rods (291) which are in sliding fit with the limiting ring (29).

4. The enzymolysis device for polypeptide production according to claim 1, wherein the top of the crushing box (8) is provided with a rotating motor (81), a leakage preventing bucket (82) and a support frame (83) arranged inside the crushing box (8), the rotating motor (81) is arranged on the side wall of the crushing box (8), the top of the support frame (83) is provided with a rotating column (84) matched with the rotating column in rotation, the rotating column (84) is sleeved with a plurality of crushing blades (85) which are arranged in a staggered mode, the output end of the rotating motor (81) is sleeved with a driving pulley (86), the bottom end of the rotating column (84) is sleeved with a driven pulley (87), and the driving pulley (86) is connected with the driven pulley (87) through belt transmission.

5. The enzymolysis device for polypeptide production according to claim 1, wherein the large-particle secondary crushing assembly (9) comprises a conveying motor (91), a conveying cylinder (92) and a conveying shaft (93) vertically arranged in the conveying cylinder (92), the conveying shaft (93) is sleeved with a spiral blade (94), the conveying motor (91) is arranged at the top of the conveying cylinder (92), the output end of the conveying motor (91) is fixedly connected with the top end of the conveying shaft (93), a material guide pipeline (95) is arranged between the top of the conveying cylinder (92) and the inside of the crushing box (8), and a material discharge channel (96) communicated with the inside of the conveying cylinder (92) is arranged at the bottom end of the inside of the crushing box (8).

6. The enzymolysis device for polypeptide production according to claim 1, wherein said homogenizing and mixing mechanism (3) comprises a stirring motor (31), an inclined discharge hopper (32) and a stirring shaft (33) disposed at the lower section of the grinding box (4), said stirring motor (31) is disposed on the sidewall of the grinding box (4), the output end of said stirring motor (31) is fixedly connected with the end of the stirring shaft (33), said stirring shaft (33) is sleeved with a plurality of stirring blades (34), and both sides of said grinding box (4) are provided with UV sterilizing lamps (35).

7. The enzymolysis device for polypeptide production according to claim 1, wherein said filtering and drying mechanism (6) comprises an open upward drying basin (61) and three fixing rods (62) distributed circumferentially below the drying basin (61), a sliding rod (63) sliding fitted with the fixing rods (62) is arranged at the top of the fixing rods (62), the top end of the sliding rod (63) is fixedly connected with the bottom end of the drying basin (61), a vibration spring (64) is sleeved on the sliding rod (63), the two ends of the vibration spring (64) are respectively fixedly connected with the top end of the fixing rod (62) and the bottom end of the drying basin (61), a vibration cylinder (65) vertically arranged is arranged below the drying basin (61), a vibration disc (66) is arranged on the output end of the vibration cylinder (65), and a support (67) is arranged at the side of the drying basin (61), the top of the support (67) is provided with a drying pipeline (68), and the drying pipeline (68) is provided with a spray nozzle (69).

8. The enzymolysis device for polypeptide production according to claim 1, wherein the top of the enzymolysis tank (7) is provided with a blending motor (71) fixedly connected with the side wall of the enzymolysis tank (7) and a feeding pipeline (72) communicated with the output end of the auger feeder (5), the enzymolysis tank (7) is provided with a feeding pipeline (73) communicated with the inside of the enzymolysis tank, the inside of the enzymolysis tank (7) is provided with two horizontally arranged connecting frames (74), a rotating shaft (75) in rotary fit with the two connecting frames (74) is arranged between the two connecting frames, the rotating shaft (75) is sleeved with blending paddles (77), the output end of the blending motor (71) is sleeved with a first belt pulley (76), the top end of the rotating shaft (75) is sleeved with a second belt pulley (78), the first belt pulley (76) is connected with the second belt pulley (78) through belt transmission, the inside bottom of enzymolysis tank (7) is equipped with molecular membrane filter (79), the lateral wall of enzymolysis tank (7) is equipped with the heater.