CN110934146A

CN110934146A - Insecticidal composition containing acetamiprid and celastrus angulatus

Info

Publication number: CN110934146A
Application number: CN201911105468.6A
Authority: CN
Inventors: 蒋兴川; 操海群; 李秀霞; 方庆奎; 盛成旺; 黄勇; 董永成; 廖敏
Original assignee: Anhui Agricultural University AHAU
Current assignee: Anhui Agricultural University AHAU
Priority date: 2019-11-12
Filing date: 2019-11-12
Publication date: 2020-03-31
Anticipated expiration: 2039-11-12
Also published as: CN110934146B

Abstract

The invention provides an insecticidal composition containing acetamiprid and celastrus angulatus, wherein the weight ratio of the acetamiprid to the celastrus angulatus is 15: 1-1: 30, preferably 15: 1 and 1: 5. The composition has obvious synergistic effect, can reduce the use amount of medicaments, reduce the cost, reduce the harm to the environment and is beneficial to delaying the generation of the drug resistance of the insect spodoptera frugiperda.

Description

Insecticidal composition containing acetamiprid and celastrus angulatus

Technical Field

The invention belongs to the field of agricultural chemistry, and particularly relates to an insecticidal composition containing acetamiprid and celastrus angulatus.

Background

Spodoptera frugiperda is a typical long-distance migratory flying pest and can be harmful to more than 80 plants such as grain crops (corn, rice, wheat and the like), economic crops (fruit trees, vegetables, flowers, cotton, peanuts and the like) and the like. Spodoptera frugiperda has become an important harm factor of local corn after 1 month in 2019 and invades the western region of Yunnan in China, and poses a threat to crop production in various provinces in southern China. The chemical pesticide has the characteristics of high efficiency, quick effect and convenient use, and is a main means for emergent prevention and control of explosive and sudden crop diseases and insect pests.

In addition, in order to overcome the defects of large dosage of a single pesticide, serious environmental pollution, high generation speed of germ resistance and the like, the invention provides an efficient and safe insecticidal composition containing acetamiprid and celastrus angulatus. Under the optimal proportion, the composition has obvious synergistic effect, reduces the dosage of the medicament under the condition of ensuring the optimal control effect, reduces the cost, reduces the harm to the environment and is beneficial to delaying the generation of drug resistance.

The prior art does not report the combined use of acetamiprid and celastrus angulatus.

Disclosure of Invention

In order to solve the problems in the prior art, the invention provides the following technical scheme:

an insecticidal composition containing acetamiprid and celastrus angulatus is characterized in that active ingredients comprise the acetamiprid and the celastrus angulatus, and the weight ratio of the acetamiprid to the celastrus angulatus is 15: 1-1: 30.

The insecticidal composition is characterized in that the weight ratio of the acetamiprid to the celastrus angulatus is 15: 1 and 1: 5.

The insecticidal composition is characterized by further comprising agriculturally acceptable auxiliaries, wherein the weight percentage of the active ingredients is 1-50%.

The insecticidal composition is characterized in that the auxiliary agent comprises a dispersing agent, an emulsifying agent, a disintegrating agent, a stabilizing agent, an antifreezing agent, a wetting agent, a synergist, a penetrating agent, a filler and a solvent.

The insecticidal composition is characterized in that: the dosage forms which can be prepared from the composition are wettable powder, water dispersible granules and aqueous emulsion.

The invention also relates to the use of the insecticidal composition according to the invention for controlling crop pests. Preferably spodoptera frugiperda.

Compared with the prior art, the invention has the following beneficial effects:

the insecticidal composition of the invention consists of two effective components with different action mechanisms, has obvious synergistic interaction after mixing, improves the control effect, and is beneficial to overcoming and delaying the generation of drug resistance of pests.

The composition of the invention is mixed with pesticide to reduce the dosage, thereby reducing the cost and lightening the pollution to the environment, having good safety and meeting the safety requirement of pesticide preparations.

The composition has excellent insecticidal and ovicidal effects on spodoptera frugiperda, and improves the control effect of the spodoptera frugiperda.

Detailed Description

The following detailed description of specific embodiments of the present invention is provided in conjunction with examples, but it should be understood that the scope of the present invention is not limited to the specific embodiments.

Indoor bioassay 1: indoor insecticidal toxicity determination of acetamiprid and celastrus angulatus on spodoptera frugiperda

Test subjects: the Spodoptera frugiperda collected in the field is bred and propagated indoors by using corn leaves, and F1 generation egg masses and hatched 2-instar larvae bred by using corn leaves are used as experimental insect sources.

The test method comprises the following steps: the insecticidal activity of the medicament is determined by adopting a leaf soaking method. Preparing a series of drug solutions to be tested with concentration, cutting corn leaves into 5cm leaves, soaking the leaves in the drug solution for 10s, naturally drying the leaves, and placing the leaves in a circular plastic box. And selecting 2-instar larvae, performing starvation treatment for 4 hours, and inoculating the treated leaves. Clear water was used as control. Larvae were treated 20 at a time, 3 replicates. And checking the survival state of the larvae 48h after treatment, judging the larvae to die by no reaction when the brush lightly touches the body surface of the larvae, recording the death number and survival number, and calculating and correcting the death rate.

The treatment-corrected mortality was converted to a probability value, the concentration was converted to a logarithmic value, and the virulence equation and the inhibitory median concentration LC50 were calculated by the least squares method. Calculating the virulence index and the cotoxicity coefficient (CTC) of the medicament according to the Sun Yunpei method.

When the CTC is less than or equal to 80, the composition shows antagonism, when the CTC is more than 80 and less than 120, the composition shows additive action, and when the CTC is more than or equal to 120, the composition shows synergistic action.

Measured virulence index (ATI) ═ standard medicament LC 50/test medicament LC50 × 100

Theoretical virulence index (TTI) ═ A agent virulence index x percent of A in the mixture + B agent virulence index x percent of B in the mixture

Co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ].

The results of the virulence determination are shown in table 1 below.

TABLE 1 indoor insecticidal toxicity assay of acetamiprid and Celastrus angulatus against Spodoptera frugiperda

As shown in Table 1, the weight ratio of the acetamiprid to the celastrus angulatus in the insecticidal composition is in the range of 1: 30-30: 1, and the composition can be used for preventing and treating Spodoptera frugiperda and has excellent insecticidal activity. The preferred weight ratio of acetamiprid to celastrus angulatus is 30: 1-15: 1, 1: 5.

Indoor bioassay 2: indoor ovicidal toxicity determination of acetamiprid and celastrus angulatus on spodoptera frugiperda

The test method comprises the following steps: the ovicidal activity of the agent was determined by egg immersion. Selecting light green egg masses which are originally laid in the current day, dispersing the egg masses into single egg grains by using a brush, and uniformly adhering the egg grains on a transparent adhesive tape to prepare egg cards; soaking the egg card in the liquid medicine for 10s, sucking the excessive liquid medicine by using filter paper, drying the liquid medicine, and then putting the liquid medicine into a 50mL centrifuge tube. Clear water was used as control. Each 20 eggs were treated, 3 replicates. After the treatment 1d, the number of hatched and unhatched eggs was checked using a split dissecting microscope, and the hatching inhibition rate was calculated and corrected. The co-toxicity coefficient calculation method is the same as above.

TABLE 2 indoor ovicidal toxicity assay of acetamiprid and Celastrus angulatus against Spodoptera frugiperda

As shown in Table 2, the weight ratio of the acetamiprid to the celastrus angulatus in the insecticidal composition is within the range of 1: 30-30: 1, the composition can be used for preventing and treating Spodoptera frugiperda, and the weight ratio of the acetamiprid to the celastrus angulatus is preferably 15: 1-1: 30.

It is found from tables 1 and 2 that when the weight ratio of the acetamiprid to the celastrus angulatus is 15: 1 and 1: 5, the composition has excellent insecticidal and ovicidal effects on spodoptera frugiperda and shows remarkable synergistic effects.

Determination of field drug Effect 1

Test subjects: spodoptera frugiperda (Spodoptera frugiperda) in corn field

The test method comprises the following steps: test set 2 medicament treatment, using clear water as a control, repeating the treatment for 3 times, setting a protection row with the area of each cell being 25m2, and randomly arranging and combining the cells. The medicine is applied in a stem and leaf spraying mode and is uniformly sprayed. And (3) respectively surveying the population density of each cell by adopting a five-point sampling method 1d before pesticide application and 7d after pesticide application, surveying 1m2 per point, recording the population number and damage condition of each point, and respectively calculating the population decline rate and the control effect.

Test agents:

agent 1: 16% acetamiprid-celastrus angulatus aqueous emulsion (15: 1)

Medicament 2: 3% acetamiprid-celastrus angulatus water dispersible granule (1: 5)

TABLE 3 field control efficacy assay results for acetamiprid and celastrus angulatus against Spodoptera frugiperda

Treatment of	Dilution factor	Prevention effect/%)
			Agent 1	2500	89.60
Medicament 2	1000	85.34
			Control	-	-

Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. All percentages in the formulation are by weight. The processing technology of each preparation of the composition is the prior art, and can be changed according to different conditions.

Claims

1. An insecticidal composition containing acetamiprid and celastrus angulatus is characterized in that active ingredients comprise the acetamiprid and the celastrus angulatus, and the weight ratio of the acetamiprid to the celastrus angulatus is 15: 1-1: 30.

2. The insecticidal composition according to claim 1, wherein the weight ratio of acetamiprid to celastrol is 15: 1, 1: 5.

3. An insecticidal composition according to any one of claims 1 to 2 further comprising agriculturally acceptable adjuvants, the active ingredients being present in an amount of from 1 to 50% by weight.

4. An insecticidal composition as defined in claim 3, wherein said adjuvants include dispersing agents, emulsifiers, disintegrants, stabilizers, anti-freezing agents, wetting agents, synergists, penetrants, fillers, solvents.

5. The insecticidal composition according to claim 4, wherein: the dosage forms which can be prepared from the composition are wettable powder, water dispersible granules and aqueous emulsion.

6. Use of the pesticidal composition according to any one of claims 1 to 5 for controlling crop pests.

7. Use according to claim 6, characterized in that it is used for the control of Spodoptera frugiperda.