CN110934140A

CN110934140A - Sex pheromone analogue disorientation technology for preventing and treating Spodoptera litura

Info

Publication number: CN110934140A
Application number: CN201911285163.8A
Authority: CN
Inventors: 梅向东; 李慧; 王留洋; 马好运; 折冬梅; 宁君
Original assignee: Institute of Plant Protection of Chinese Academy of Agricultural Sciences
Current assignee: HEBEI YIHANG NEW ENERGY TECHNOLOGY Co.,Ltd.
Priority date: 2019-12-13
Filing date: 2019-12-13
Publication date: 2020-03-31
Anticipated expiration: 2039-12-13
Also published as: CN110934140B

Abstract

The invention discloses a sex pheromone analogue disorientation technology for preventing and treating Spodoptera litura. The sex pheromone is characterized in that the disorienting agent of the Spodoptera frugiperda is cis-11-hexadecenyl-2-chloropropionate which is a sex pheromone analogue component of the Spodoptera frugiperda. Cis-11-hexadecenyl-2-chloropropionate is added into trans-rubber head to prepare the luring core of the Heliothis spinosa disorientation agent, and the luring core has good disorientation effect on Heliothis spinosa in fields. The sex attractant for the Spodoptera litura can more effectively interfere the mating between male and female Spodoptera litura, the mating rate of adults is reduced to the maximum extent, the number of reproduction is reduced, the dual effects of interference and disorientation are achieved, the aim of preventing and controlling the Spodoptera litura is achieved, and a new way is developed for green prevention and control of the Spodoptera litura.

Description

Sex pheromone analogue disorientation technology for preventing and treating Spodoptera litura

Technical Field

The invention belongs to the technical field of biological control, and particularly relates to a sex pheromone analogue disorientation technology for controlling Spodoptera litura.

Background

Spodoptera litura (Scotogramma trifolii Rottemeberg) belongs to Lepidoptera (Lepidotera) and Noctuidae, is an agricultural pest which is locally outbreak and is harmful in northern agricultural and pastoral areas in China, and the harm gradually rises in recent years, and Spodoptera litura is a polyphagic pest which can harm peas, flax, broad beans, sugar beets, potatoes and the like. The adult insects emerge at night in the daytime and are hidden in soil seams or the back of plant leaves, and the adult insects move at night, and the larvae have the characteristics of concealment, outbreak, migration hazard and the like. Through analysis of dynamic growth and migratory flight sources under the overwintering generation of Spodoptera litura, the overwintering state of the Spodoptera litura is found to have an obvious migratory flight phenomenon. The agricultural control comprises deep tillage of soil in autumn and winter, adherence to winter irrigation, cleaning of a field garden, weed removal and reduction of overwintering base number; the physical prevention and control mainly comprises the steps of trapping and killing the plants by using light traps so as to reduce the population quantity of the plants; the main control agents are BT emulsion, chlorbenzuron, etc. With the enhancement of awareness of food safety, biological pest control is becoming an urgent problem. Therefore, the urgent need of solving the current environmental pollution and food safety crisis is to find an efficient and green prevention and control technology which is specific in prevention and control objects, harmless to the environment and natural enemies, free of residues and safe to people and livestock. The insect disorientation agent has the characteristics of high efficiency, greenness and environmental protection, releases artificially synthesized disorientation compounds through the lure core, slowly releases the disorientation compounds to the field, can effectively interfere the orientation of males to females, and reduces the encounter probability of the males and females, thereby destroying the mating of the males and the females and finally achieving the purpose of prevention and treatment.

Disclosure of Invention

The invention aims to provide a sex pheromone analogue disorientation technology for preventing and treating Spodoptera litura. Various analogues are obtained by optimizing the chemical structure of the pheromone active ingredient, so that various components of the sex pheromone are more stable, and the release rate is slowed down. The disorientation agent in the method has strong specificity and has no influence on natural enemies in nature; the raw materials of the release device are easy to obtain, simple and feasible; the influence of natural conditions is small; the mating interference ability is strong; the persistent period is long, the control effect is outstanding, the mating rate of the Spodoptera litura can be reduced by 80%, and the population quantity of the Spodoptera litura can be reduced by more than 80% by long-term release.

In order to achieve the purpose, the following technical scheme is adopted:

a sex pheromone analogue disorientation control technology for Spodoptera frugiperda is disclosed, wherein the sex pheromone analogue of Spodoptera frugiperda is cis-11-hexadecenyl-2-chloropropionate.

The mass of the sex pheromone analogue cis-11-hexadecenyl-2-chloropropionate of the Spodoptera litura for disorientation is 0.5-2 mg;

preferably, the sex pheromone analogue cis-11-hexadecenyl-2-chloropropionate used for disorientation is 1-2 mg;

most preferably, the sex pheromone analogue cis-11-hexadecenyl-2-chloropropionate used for disorientation is 1.5 mg.

The sex pheromone analogue can be used for preparing the athyria spinosa disorientation lure, and the preparation method comprises the following steps: adding n-hexane solution containing Heliothis spinosa sex pheromone analogue cis-11-hexadecenyl-2-chloropropionate into trans-rubber head to obtain disorientation agent core, wherein the effective component of single disorientation agent lure core is 0.5-2mg, and storing the lure core in a refrigerator at-20 deg.C for use. The lure core of the disorientation agent is arranged in a box-shaped trap, the trap is hung in a region where the Spodoptera frugiperda occurs or is likely to occur, and the placement density of the trap in the field is 300-²The dosage of the sex information analog of the Spodoptera litura for disorientation is 150-1000mg/hm²。

Compared with the prior art, the invention has the following beneficial effects: compared with the sex pheromone of the Spodoptera litura, the sex pheromone analogue of the Spodoptera litura is more stable, can slow down the release rate and can achieve the disorientation effect. The sex information analogue disorienting agent is used to interfere the mating of the imagoes and reduce the egg laying amount of the overwintering generation imagoes, so that the number of the 1 st generation larva, the 2 nd generation larva and the imagoes is reduced, meanwhile, the imagoes trapped by the trap physically kill the imagoes, and the number of pests is correspondingly reduced. In addition, the sex pheromone analogue of the Spodoptera litura has strong specificity, does not generate resistance, has no harm to natural enemies, is nontoxic to human and livestock, is environment-friendly, can be accurately applied to the interference mating of the Spodoptera litura, and has important significance for comprehensively treating the Spodoptera litura.

Drawings

FIG. 1 is a schematic diagram of the chemical structure of cis-11-hexadecenyl-2-chloropropionate

FIG. 2 is a schematic diagram of the synthetic route of cis-11-hexadecenyl-2-chloropropionate

Detailed Description

The present invention is described in detail below with reference to the drawings and examples, but it should be understood that the scope of the present invention is not limited to the specific embodiments.

EXAMPLE 1 preparation of cis-11-hexadecenyl-2-chloropropionate

The synthetic route of the compound cis-11-hexadecenyl-2-chloropropionate is shown in figure 2.

Dissolving 0.5g (2.08mmol) of cis-11-hexadecenol in 20mL of anhydrous dichloromethane, adding 0.42g (4.16mmol) of Triethylamine (TEA) as an acid-binding agent, stirring for 10min under an ice bath condition, slowly dropwise adding a dichloromethane solution containing 0.4g (3.12mmol) of 2-chloropropionyl chloride, reacting the system for about 30min, removing the ice bath, slowly heating to room temperature, continuing to react for about 2h, monitoring by TLC in the reaction process, adding a small amount of deionized water after the raw materials are not reduced, and quenching the reaction. The extract was washed with a saturated saline solution, dried over anhydrous magnesium sulfate for 40min, filtered and rotary-evaporated under reduced pressure. Silica gel column chromatography (ethyl acetate: petroleum ether ═ 1: 20) afforded the title compound. The product was a yellow oil in 73% yield.

EXAMPLE 2 Interval bioactivity assay

Vegetable fields with basically the same sowing time, variety and daily management measures and consistent growth vigor are selected as test areas. Will be located at 0.4hm of the upper tuyere²The vegetable field is used as a contrast area, 3 traps containing sex pheromone lure cores of the Spodoptera scoparia are respectively and uniformly placed in the center of the vegetable field in a regular triangle way as contrast, and the height of the traps is 100-150 cm; 0.4hm of lower tuyere²The vegetable field is used as a disorientation area, 300-500 box-shaped trapping cores containing sex pheromone analogue disorientation agents of the Spodoptera nutans are evenly placed in each hectare3 traps containing sex pheromone attracting cores of the Spodoptera litura are respectively and uniformly placed in the center of the disorientation area in a regular triangle manner to be used as monitoring; 0.2hm in the middle²The vegetable field is used as an isolation area without any traps.

Wherein 3 replicates of each disorienting agent formula are set in the disorienting area, the disorienting rate is averaged (analyzed by SPSS data processing software), and the specific dosage and placement quantity of the disorienting agent and the disorienting rate effect are shown in table 1.

(wherein the expression of the disorientation rate (%) is (1-amount of moth-trapping in the disorientation area/amount of moth-trapping in the control area) × 100%)

TABLE 1

The experimental result shows that the disorientation rate is gradually increased along with the increase of the dosage of the athetis lepigone disorientation agent by comparing the formulas 1, 2, 3 and 4; comparing the formulas 3, 5, 6 and 7, it can be seen that the disorientation rate is gradually increased with the increase of the number of traps; as can be seen by comparing the formulations 3, 4, 6 and 7, although the disorientation rate tends to increase with the dosage of the disorientation agent and the number of traps, the disorientation rate is increased to a certain extent at P>0.05, there is no significant difference, and from the economical point of view of agricultural use, the dosage of the disorienting agent added to a single trap is 1.5mg, and the number of traps set is 500 per hm²The disorientation effect is the best, the disorientation rate can reach more than 87%, and simultaneously under the guidance of the scheme, the purposes of the lowest cost and the best prevention and treatment effect are achieved. The cis-11-hexadecenyl-2-chloropropionate is used as a disorientation agent of the Spodoptera frugiperda, and provides a powerful and feasible strategy for green and efficient control of the Spodoptera frugiperda.

The above disclosure is only for the specific embodiment of the present invention, but the present invention is not limited thereto, and any variations that can be made by those skilled in the art should fall within the scope of the present invention.

Claims

1. A sex pheromone analog disorientation technology for preventing and treating Spodoptera frugiperda is characterized in that the sex pheromone analog disorientation agent of Spodoptera frugiperda is cis-11-hexadecylene-2-chloropropionate.

2. A sex pheromone analog disorientation technology for preventing and treating the Spodoptera frugiperda is characterized in that a sex pheromone analog cis-11-hexadecylene-2-chloropropionate of the Spodoptera frugiperda is added into a trans-rubber head to prepare a Spodoptera frugiperda disorientation lure.

3. The sex pheromone analog disorientation technology for controlling the noctuids according to claim 1, wherein the sex pheromone analog cis-11-hexadecenyl-2-chloropropionate contained in the single noctuid disorientation lure is 0.5-2mg in mass.

4. The sex pheromone analog disorientation technique for preventing and treating Heliothis volubilis according to claim 1, wherein the lure is placed in a box-type trap, the trap is placed in a region where Heliothis volubilis occurs or is likely to occur, and the number of the trap is 300-²。

5. The sex pheromone analog disorientation technique for preventing and treating Heliothis spinosa as claimed in claim 1, wherein the amount of disorientation agent for preventing and treating Heliothis spinosa is 150-1000mg/hm²。