CN110923114A

CN110923114A - 96 supplementary application of sample sampling device of hole PCR board

Info

Publication number: CN110923114A
Application number: CN201911137968.8A
Authority: CN
Inventors: 李学刚; 孙淑娟; 李延坤; 姜辉
Original assignee: Binzhou Agricultural And Rural Bureau
Current assignee: Binzhou Agricultural And Rural Bureau
Priority date: 2019-11-20
Filing date: 2019-11-20
Publication date: 2020-03-27

Abstract

The invention discloses an auxiliary sample adding and sampling device for a 96-hole PCR plate, which belongs to the technical field of polymerase chain reaction experimental equipment and comprises a placing box, wherein a placing opening is formed in the middle of the top end of the placing box, a PCR plate is placed in the placing opening, and positioning mechanisms are arranged at four corners of the placing opening, can shelter from laser, laser illumination is outside to the work area when avoiding using.

Description

96 supplementary application of sample sampling device of hole PCR board

Technical Field

The invention relates to the technical field of polymerase chain reaction experimental equipment, in particular to a 96-hole PCR plate auxiliary sample adding and sampling device.

Background

The Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying and amplifying specific DNA fragments, and can be regarded as special DNA replication in vitro, and the biggest characteristic of the PCR is that trace amount of DNA can be greatly increased. Therefore, even if only a single spot of DNA can be isolated from the hairs, skin or blood left by the murder in the murder case, or the remains of the historic creatures and the historical characters in the fossil, the DNA can be amplified by PCR and compared.

In the polymerase chain reaction, the PCR plate serves as a carrier for primers, Taq DNA polymerase, dNTPs, template nucleic acid, Mg, buffer, and the like, which participate in the amplification reaction. The PCR plate is provided with a plurality of reaction tubes with closed bottoms and open tops, and the commonly used PCR plates are divided into 96-well PCR plates and 384-well PCR plates according to the different numbers of the reaction tubes. In the case of performing the polymerase chain reaction, it is necessary to separately load the components such as primers, TaqDNA polymerase, dNTP, nucleic acid template, Mg, buffer solution, etc. into each reaction tube of the PCR plate, and then add different nucleic acid templates into each reaction tube. In order to prevent errors, a row of transverse marks and a column of longitudinal marks are arranged on the PCR plate and used for positioning the reaction tubes by operators.

However, because the number of reaction tubes is too large, the operator can easily read the wrong identification, and the nucleic acid template is added in a wrong way, so that the experiment fails. Moreover, because the distance between the reaction tubes is small, marks cannot be made at each reaction tube opening.

Based on the above, the invention designs an auxiliary sample adding and sampling device for a 96-well PCR plate, so as to solve the problems.

Disclosure of Invention

The invention aims to provide an auxiliary sample adding and sampling device for a 96-well PCR plate, which aims to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: a96-hole PCR plate auxiliary sample adding and sampling device comprises a placing box, wherein a placing opening is formed in the middle of the top end of the placing box, a PCR plate is placed in the placing opening, positioning mechanisms are arranged at four corners of the placing opening, and the PCR plate is positioned and placed in the placing opening through the positioning mechanisms;

the automatic test tube placing device is characterized in that a first mounting groove is longitudinally formed in the top end of the placing box close to the left side of the placing opening, a second mounting groove is transversely formed in the top end of the placing box close to the front side of the placing opening, laser lamp assemblies are mounted in the first mounting groove and the second mounting groove, the laser lamp assemblies are provided with multiple groups, the multiple groups of laser lamp assemblies positioned on the first mounting groove are aligned with test tube holes transversely distributed on a PCR plate, the multiple groups of laser lamp assemblies positioned on the second mounting groove are aligned with test tube holes longitudinally distributed on the PCR plate, a first light barrier is arranged on the rear side of the top end of the placing box, and a second light;

four corners of the bottom end of the placing box are provided with buffering support legs.

Preferably, the laser lamp component comprises a sliding block, a laser lamp is arranged on one side, close to the placing port, of the sliding block, a button switch is arranged on the laser lamp, a limiting screw rod is arranged at the top end of the sliding block, and a threaded through hole matched with the limiting screw rod is formed in the sliding block.

Preferably, the laser lamp is a battery-replaceable mini laser lamp.

Preferably, the positioning mechanism comprises a fixed plate, a threaded rod is connected to the fixed plate in a penetrating manner, and a clamping gasket is arranged at the bottom end of the threaded rod.

Preferably, the clamping washer is made of elastic plastic.

Preferably, the buffering stabilizer blade is including the sleeve pipe, the sleeve pipe bottom is provided with the sucking disc, it is provided with the support column to slide in the sheathed tube hole, be provided with the spring between support column bottom and the sheathed tube hole, the support column top with place box bottom rigid coupling.

Compared with the prior art, the invention has the beneficial effects that: according to the invention, through the two groups of laser lamp assemblies which are vertically arranged, a user can more accurately find the reagent bottles on the PCR plate, so that the experiment failure caused by the misoperation of mistakenly-looking reagent bottles is avoided, the laser lamp assemblies can flexibly adjust the positions, so that the accurate alignment of the laser lamp assemblies and the reagent holes can be kept according to the size change of the PCR plate, the practicability is stronger, the PCR plate can be stably fixed through the arranged positioning mechanism, the laser alignment is convenient, the buffering shock resistance of the device can be enhanced through the arranged buffering support legs, and the laser can be shielded through the arranged first light barrier and the second light barrier, so that the laser is prevented from irradiating outside a working area when in use.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a top view of the present invention;

FIG. 2 is a top view of the present invention with the PCR plate removed;

FIG. 3 is a three-dimensional schematic view of the present invention with the PCR plate and laser light assembly removed;

FIG. 4 is an enlarged view taken at A in FIG. 1 according to the present invention;

FIG. 5 is an enlarged view of the invention at B of FIG. 3;

FIG. 6 is a schematic view of a buffer foot according to the present invention.

In the drawings, the components represented by the respective reference numerals are listed below:

1-placing box, 2-placing opening, 3-PCR plate, 4-positioning mechanism, 5-first mounting groove, 6-second mounting groove, 7-laser lamp assembly, 8-first light barrier, 9-second light barrier, 10-buffer supporting leg, 41-fixing plate, 42-threaded rod, 43-clamping gasket, 71-sliding block, 72-limiting screw rod, 73-laser lamp, 74-button switch, 101-sleeve, 102-sucking disc, 103-supporting column and 104-spring.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-6, the present invention provides a technical solution: a96-hole PCR plate auxiliary sample adding and sampling device comprises a placing box 1, wherein a placing opening 2 is formed in the middle of the top end of the placing box 1, a PCR plate 3 is placed in the placing opening 2, positioning mechanisms 4 are arranged at four corners of the placing opening 2, and the PCR plate 3 is positioned and placed in the placing opening 2 through the positioning mechanisms 4;

a first mounting groove 5 is longitudinally arranged at the top end of the placing box 1 close to the left side of the placing port 2, a second mounting groove 6 is transversely arranged at the top end of the placing box 1 close to the front side of the placing port 2, laser lamp assemblies 7 are mounted in the first mounting groove 5 and the second mounting groove 6, a plurality of groups of laser lamp assemblies 7 are arranged on the first mounting groove 5 and are aligned with test tube holes transversely distributed on the PCR plate 3, a plurality of groups of laser lamp assemblies 7 on the second mounting groove 6 are arranged and are aligned with test tube holes longitudinally distributed on the PCR plate 3, a first light barrier 8 is arranged at the rear side of the top end of the placing box 1, and a second light barrier 9 is arranged at the right side of the top end of;

four corners of the bottom end of the placing box 1 are provided with buffering support legs 10.

As shown in fig. 4, the laser lamp assembly 7 includes a sliding block 71, a laser lamp 73 is disposed on one side of the sliding block 71 close to the placing port 2, a button switch 74 is disposed on the laser lamp 73, a limit screw 72 is disposed on the top end of the sliding block 71, and a threaded through hole matched with the limit screw 72 is disposed on the sliding block 71.

Wherein, the laser lamp 73 is a battery-replaceable mini laser lamp.

As shown in fig. 5, the positioning mechanism 4 includes a fixing plate 41, a threaded rod 42 is connected to the fixing plate 41, and a clamping pad 43 is disposed at a bottom end of the threaded rod 42.

The clamping pad 43 is made of elastic plastic.

As shown in fig. 6, the buffering support leg 10 includes a sleeve 101, a suction cup 102 is disposed at the bottom end of the sleeve 101, a supporting column 103 is slidably disposed in an inner hole of the sleeve 101, a spring 104 is disposed between the bottom end of the supporting column 103 and the inner hole of the sleeve 101, and the top end of the supporting column 103 is fixedly connected to the bottom end of the placing box 1.

One specific application of this embodiment is: the device is an auxiliary sample adding and sampling device for a 96-hole PCR plate, is mainly suitable for the 96-hole PCR plate, and is provided with a row of 1-12 digital marks and a column of A-H letter marks. During the use, arrange the PCR board in placing mouthful 2, through screwing threaded rod 42, press from both sides the white limit of PCR board tightly, then the user is according to horizontal array through-hole on the PCR board, adjust laser lamp subassembly 7 on the first mounting groove 5 one by one, make its horizontal alignment PCR board go up the test tube through-hole, through rotating limit screw 71, fix or loosen laser lamp subassembly 7, adjust laser lamp subassembly 7 on the second mounting groove 6 on the same reason, make its vertical alignment PCR board go up the test tube through-hole. During the use, according to test tube position serial number (vertical coordinate is a certain number in 1 ~ 12, horizontal coordinate is a certain letter in A ~ H), press button switch 74 on the laser lamp subassembly 7 of corresponding position respectively for laser lamp 73 sends bright light, and the position that light vertically and horizontally intersects is the test tube position that needs promptly.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. The utility model provides a 96 hole PCR board sample loading sampling device that assists which characterized in that: the device comprises a placing box (1), wherein a placing opening (2) is formed in the middle of the top end of the placing box (1), a PCR plate (3) is placed in the placing opening (2), positioning mechanisms (4) are arranged at four corners of the placing opening (2), and the PCR plate (3) is placed in the placing opening (2) in a positioning mode through the positioning mechanisms (4);

a first mounting groove (5) is longitudinally formed in the top end of the placing box (1) close to the left side of the placing port (2), a second mounting groove (6) is transversely formed in the top end of the placing box (1) close to the front side of the placing port (2), laser lamp assemblies (7) are mounted in the first mounting groove (5) and the second mounting groove (6), a plurality of groups of laser lamp assemblies (7) are arranged on the first mounting groove (5) and are aligned with test tube holes transversely distributed on the PCR plate (3), a plurality of groups of laser lamp assemblies (7) are arranged on the second mounting groove (6) and are aligned with test tube holes longitudinally distributed on the PCR plate (3), a first light barrier (8) is arranged on the rear side of the top end of the placing box (1), and a second light barrier (9) is arranged on the right side of the top end of the placing box (1);

four corners of the bottom end of the placing box (1) are provided with buffering support legs (10).

2. The sample-adding and sampling device for assisting 96-well PCR plate according to claim 1, wherein: laser banks spare (7) are including slider (71), slider (71) are close to and place mouthful (2) one side and are provided with laser lamp (73), be equipped with button switch (74) on laser lamp (73), slider (71) top is provided with stop screw (71), and is equipped with the screw thread through-hole that matches stop screw (71) on slider (71).

3. The sample-adding and sampling device for assisting 96-well PCR plate according to claim 2, wherein: the laser lamp (73) is a mini laser lamp with a replaceable battery.

4. The sample-adding and sampling device for assisting 96-well PCR plate according to claim 1, wherein: the positioning mechanism (4) comprises a fixing plate (41), a threaded rod (42) is connected to the fixing plate (41) in a penetrating mode, and a clamping gasket (43) is arranged at the bottom end of the threaded rod (42).

5. The sample-adding and sampling device for assisting 96-well PCR plate according to claim 4, wherein: the clamping gasket (43) is made of elastic plastic.

6. The sample-adding and sampling device for assisting 96-well PCR plate according to claim 1, wherein: buffering stabilizer blade (10) is including sleeve pipe (101), sleeve pipe (101) bottom is provided with sucking disc (102), it is provided with support column (103) to slide in the hole of sleeve pipe (101), be provided with spring (104) between the hole of support column (103) bottom and sleeve pipe (101), support column (103) top with place box (1) bottom rigid coupling.