CN110916188A - Composition containing probiotics and konjak extract and preparation method and application thereof - Google Patents
Composition containing probiotics and konjak extract and preparation method and application thereof Download PDFInfo
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- CN110916188A CN110916188A CN201911335089.6A CN201911335089A CN110916188A CN 110916188 A CN110916188 A CN 110916188A CN 201911335089 A CN201911335089 A CN 201911335089A CN 110916188 A CN110916188 A CN 110916188A
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- 229920002752 Konjac Polymers 0.000 title claims abstract description 93
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- 239000006041 probiotic Substances 0.000 title claims abstract description 39
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title abstract description 6
- 238000000605 extraction Methods 0.000 title description 2
- 230000000694 effects Effects 0.000 claims abstract description 25
- 239000002994 raw material Substances 0.000 claims abstract description 21
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims abstract description 17
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- 235000013305 food Nutrition 0.000 claims abstract description 11
- 230000036541 health Effects 0.000 claims abstract description 11
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- 239000000252 konjac Substances 0.000 claims description 32
- 238000007873 sieving Methods 0.000 claims description 19
- 238000010438 heat treatment Methods 0.000 claims description 18
- 238000004108 freeze drying Methods 0.000 claims description 17
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- 230000007062 hydrolysis Effects 0.000 claims description 14
- 238000006460 hydrolysis reaction Methods 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 14
- MMXZSJMASHPLLR-UHFFFAOYSA-N pyrroloquinoline quinone Chemical group C12=C(C(O)=O)C=C(C(O)=O)N=C2C(=O)C(=O)C2=C1NC(C(=O)O)=C2 MMXZSJMASHPLLR-UHFFFAOYSA-N 0.000 claims description 14
- 108090000145 Bacillolysin Proteins 0.000 claims description 11
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- 238000001291 vacuum drying Methods 0.000 claims description 9
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- 239000000843 powder Substances 0.000 description 15
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- 241001465754 Metazoa Species 0.000 description 9
- QGMRQYFBGABWDR-UHFFFAOYSA-M Pentobarbital sodium Chemical compound [Na+].CCCC(C)C1(CC)C(=O)NC(=O)[N-]C1=O QGMRQYFBGABWDR-UHFFFAOYSA-M 0.000 description 7
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- 238000010521 absorption reaction Methods 0.000 description 3
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 3
- 229940029339 inulin Drugs 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229960001412 pentobarbital Drugs 0.000 description 3
- 239000004375 Dextrin Substances 0.000 description 2
- 229920001353 Dextrin Polymers 0.000 description 2
- 241000194033 Enterococcus Species 0.000 description 2
- 238000012449 Kunming mouse Methods 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 235000019425 dextrin Nutrition 0.000 description 2
- 235000003642 hunger Nutrition 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 229940039696 lactobacillus Drugs 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 201000010538 Lactose Intolerance Diseases 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
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- 229920002678 cellulose Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000002641 glycemic effect Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
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- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004719 natural immunity Effects 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to a composition containing probiotics and konjak extract and a preparation method and application thereof, wherein the composition containing the probiotics and the konjak extract adopts lactobacillus rhamnosus, the konjak extract and vitamin coenzyme as raw materials and is prepared by proper proportion, and the finally prepared composition containing the probiotics and the konjak extract has better effects of regulating intestinal flora and improving sleep under the synergistic action of the raw material components, and the composition containing the probiotics and the konjak extract is added into health products, foods or medicines, can effectively improve sub-health problems caused by intestinal flora, immune dysfunction, sleep disorder and the like by supplementing probiotics in vitro and proliferating intestinal beneficial bacteria in vivo, and does not contain hormone and sweetening agent, is safe and nontoxic, has better social benefit and economic benefit.
Description
Technical Field
The invention belongs to the technical field of compositions, and particularly relates to a composition containing probiotics and a konjak extract, and a preparation method and application thereof.
Background
With the improvement of life quality, people pay more and more attention to their health. At present, the main disease types harmful to health in China are cardiovascular and cerebrovascular health diseases and digestive system health diseases, the digestive system is the basis and origin of human health, and the physical condition of people is better and better only if the digestive system is healthy. While intestinal problems are the most common of the problems of the digestive system.
The application of probiotics in food and medicine is very wide, and the main effects are shown in the following aspects: inhibiting the propagation of pathogenic bacteria and conditioned pathogenic bacteria in the intestinal tract; synthesizing some vitamins to promote lactose decomposition and absorption; decomposing cellulose, creating a healthy intestinal environment, and contributing to the treatment of constipation and diarrhea; improve the digestion and absorption of the human body; prevention of lactose intolerance; in addition, the acidic state of the intestinal tract can be maintained, and the absorption of calcium is facilitated. Medical research shows that lactic acid bacteria serve as beneficial bacteria in human bodies among thousands of bacteria in human intestinal tracts, and the micro-ecological balance is restored and maintained by supplementing the beneficial bacteria in the intestinal tracts, so that the effect of regulating the host intestinal flora is achieved. In addition, the lactobacillus is fixedly planted in the intestinal tract of a human body, is equivalent to natural immunity, can activate the phagocytic activity of the body, is used as immunogen to induce specific and non-specific immune reaction, generates specific antibody and sensitized lymphocyte, regulates the immune response of the body, enhances the immunity of the body, and improves the anti-infection capacity.
Konjak has all the beneficial physiological functions in dietary fiber definition without side effects, and has good taste and good compatibility with other food materials and food industry raw materials. The konjak extract can effectively inhibit the activity of amylase, thereby reducing the glycemic index of food, reducing the sugar intake of human body, lowering the peak value of blood sugar, relieving the pancreatic gland burden, and keeping proper hunger feeling without hunger, which has very special significance to human health.
Disclosure of Invention
In order to solve the above problems of the prior art, the present invention provides a composition comprising probiotics and a konjac extract, and a method for preparing the same. The composition is suitable for food, medicine or health care products, does not contain hormone or sweetener, is suitable for long-term use, and has good effects of regulating intestinal flora and improving sleep.
The technical scheme adopted by the invention is as follows:
a composition contains probiotic bacteria and rhizoma Amorphophalli extract, and comprises Lactobacillus rhamnosus, rhizoma Amorphophalli extract and vitamin coenzyme.
Further preferably, the raw material components of the composition containing probiotics and konjak extract comprise:
10-30 parts of lactobacillus rhamnosus;
20-35 parts by weight of konjak extract;
1-2 parts of vitamin coenzyme.
Further preferably, the raw material components of the composition containing probiotics and konjak extract comprise:
20 parts of lactobacillus rhamnosus;
28 parts of konjak extract;
1.5 parts of vitamin coenzyme.
Further preferably, the raw material components of the composition containing the probiotics and the konjak extract also comprise 33-69 parts by weight of food auxiliary materials.
The food auxiliary material is one or a mixture of more of inulin, xylo-oligosaccharide and resistant dextrin.
The lactobacillus rhamnosus is lactobacillus rhamnosus XM106, which is preserved in the microbiological laboratory of the national institute of Life sciences of university of science and technology in 2017, 3 months and 10 days, and the preservation unit address is as follows: anhui province Hefei city Jinzhai Lu 96 with the collection number of XM 106.
The vitamin coenzyme is pyrroloquinoline quinone.
The konjak extract is prepared by the following method:
(1) freeze drying rhizoma Amorphophalli, pulverizing, sieving, soaking in water, and heating;
(2) cooling the konjac subjected to the heating treatment in the step (1), adding neutral protease for first enzymolysis, adjusting the pH value to acidity, adding pepsin for second enzymolysis, adjusting the pH value to neutrality, and filtering to obtain a hydrolysis extract;
(3) and drying the hydrolysis extracting solution to obtain the konjak extract.
In the step (1), the temperature for carrying out the freeze drying is-55 to-25 ℃, and the time for carrying out the freeze drying is 2 to 5 hours;
the particle size after crushing and sieving is 30-50 meshes, the temperature for carrying out the heating treatment is 55-75 ℃, and the time for carrying out the heating treatment is 45-90 min.
In the step (2), the temperature is reduced to 50-60 ℃;
the enzyme activity of the neutral protease is 20-40 ten thousand IU, the addition amount of the neutral protease accounts for 0.05-0.08% of the dry weight of the konjak, the temperature for carrying out the first enzymolysis is 50-60 ℃, and the time for carrying out the first enzymolysis is 4-8 h; the enzyme activity of the pepsin is 5-20 ten thousand IU, the addition amount of the pepsin accounts for 0.05-0.08% of the dry weight of the konjak, the enzymolysis temperature is 40-50 ℃, and the enzymolysis time is 12-24 h.
In the step (3), the drying is to freeze and dry the extracting solution firstly and then to carry out vacuum drying.
The temperature for carrying out the freeze drying is-30 to-25 ℃, and the time for carrying out the freeze drying is 2 to 5 hours;
the temperature for carrying out the vacuum drying is 20-40 ℃, and the time for carrying out the vacuum drying is 2-5 h.
The preparation method of the composition containing the probiotics and the konjak extract comprises the following steps:
weighing the raw material components, and fully and uniformly mixing to obtain the composition containing the probiotics and the konjak extract.
The composition is applied to health care products for regulating intestinal flora and improving sleep. The composition may be used in food and pharmaceutical products.
The invention has the beneficial effects that:
the composition containing the probiotics and the konjak extract is prepared by adopting lactobacillus rhamnosus, the konjak extract and vitamin coenzyme as raw materials and carrying out proper proportioning, has better effects of regulating intestinal flora and improving sleep under the synergistic effect of the raw material components, can effectively improve sub-health problems caused by intestinal flora, immune dysfunction, sleep disorder and the like by adding the composition containing the probiotics and the konjak extract into health products, foods or medicines and supplementing the probiotics in vitro and proliferating the intestinal beneficial bacteria in vivo, and is safe and non-toxic without hormone and sweetener and has better social benefit and economic benefit.
Detailed Description
The present invention will be described in detail with reference to specific examples. In the following examples, 1 part by weight represents 1 g.
Example 1
The embodiment provides a composition containing probiotics and a konjak extract, and the raw material components comprise:
20 parts of lactobacillus rhamnosus XM 106;
25 parts by weight of konjak extract;
1.5 parts by weight of pyrroloquinoline quinone;
resistant dextrin, 53.5 parts by weight.
Wherein the konjak extract is prepared by the following method:
(1) freeze drying rhizoma Amorphophalli at-30 deg.C, pulverizing, sieving to obtain 30 mesh fine powder, soaking in 2 times of water, and heating at 55 deg.C for 90 min;
(2) cooling the konjac subjected to the heating treatment in the step (1) to 50 ℃, adding neutral protease accounting for 0.05% of the dry weight of the konjac (the mass of the konjac fine powder obtained after the sieving treatment in the step 1) and having 40 ten thousand IU of enzyme activity into the konjac for carrying out first enzymolysis for 8 hours at 50 ℃, adjusting the pH value to 2 to be acidic, adding pepsin accounting for 0.05% of the dry weight of the konjac (the mass of the konjac fine powder obtained after the sieving treatment in the step 1) and having 20 ten thousand IU of enzyme activity into the konjac for carrying out second enzymolysis for 24 hours, adjusting the pH value to 6.5 to be neutral by using a sodium bicarbonate solution, and filtering the mixture to obtain a hydrolysis extract;
(3) freeze-drying the hydrolysis extract at-55 deg.C for 5h, and vacuum-drying at 20 deg.C for 5h to obtain rhizoma Amorphophalli extract.
The composition containing the probiotics and the konjak extract is prepared by the following method:
weighing the raw material components, and fully and uniformly mixing to obtain the composition containing the probiotics and the konjak extract.
Example 2
The embodiment provides a composition containing probiotics and a konjak extract, and the raw material components comprise:
25 parts of lactobacillus rhamnosus XM 106;
32 parts of konjak extract;
2 parts of pyrroloquinoline quinone;
inulin 41 parts by weight.
Wherein the konjak extract is prepared by the following method:
(1) freeze drying rhizoma Amorphophalli at-25 deg.C, pulverizing, sieving to obtain 50 mesh fine powder, soaking in 4 times of water, and heating at 75 deg.C for 45 min;
(2) cooling the konjac subjected to the heating treatment in the step (1) to 60 ℃, adding neutral protease accounting for 0.08% of the dry weight of the konjac (the mass of the konjac fine powder obtained after the sieving treatment in the step 1) and having an enzyme activity of 40 ten thousand IU into the konjac for carrying out first enzymolysis for 4 hours at 60 ℃, adjusting the pH value to 3 to be acidic, adding pepsin accounting for 0.08% of the dry weight of the konjac (the mass of the konjac fine powder obtained after the sieving treatment in the step 1) and having an enzyme activity of 20 ten thousand IU into the konjac for carrying out second enzymolysis for 24 hours, adjusting the pH value to 7.5 by using a sodium bicarbonate solution to be neutral, and filtering the mixture to obtain a hydrolysis extract;
(3) freeze-drying the hydrolysis extract at-25 deg.C for 2 hr, and vacuum-drying at 40 deg.C for 2 hr to obtain rhizoma Amorphophalli extract.
The composition containing the probiotics and the konjak extract is prepared by the following method:
weighing the raw material components, and fully and uniformly mixing to obtain the composition containing the probiotics and the konjak extract.
Example 3
The embodiment provides a composition containing probiotics and a konjak extract, and the raw material components comprise:
20 parts of lactobacillus rhamnosus XM 106;
28 parts of konjak extract;
1.5 parts by weight of pyrroloquinoline quinone;
inulin, 50.5 parts by weight.
Wherein the konjak extract is prepared by the following method:
(1) freeze drying rhizoma Amorphophalli at-28 deg.C, pulverizing, sieving to obtain 40 mesh fine powder, soaking in 3 times of water, and heating at 65 deg.C for 70 min;
(2) cooling the konjac subjected to the heating treatment in the step (1) to 55 ℃, adding neutral protease accounting for 0.065% of the dry weight of the konjac (the mass of the konjac fine powder obtained after the sieving treatment in the step 1) and having an enzyme activity of 30 ten thousand IU at 55 ℃ for carrying out first enzymolysis for 6 hours, adjusting the pH value to 2 to be acidic, adding pepsin accounting for 0.065% of the dry weight of the konjac (the mass of the konjac fine powder obtained after the sieving treatment in the step 1) and having an enzyme activity of 12.5 ten thousand IU for carrying out second enzymolysis for 18 hours, adjusting the pH value to 7 to be neutral by using a sodium bicarbonate solution, and filtering to obtain a hydrolysis extract;
(3) freeze-drying the hydrolysis extract at-40 deg.C, and vacuum drying at 30 deg.C for 3.5h to obtain rhizoma Amorphophalli extract.
The composition containing the probiotics and the konjak extract is prepared by the following method:
weighing the raw material components, and fully and uniformly mixing to obtain the composition containing the probiotics and the konjak extract.
Example 4
The embodiment provides a composition containing probiotics and a konjak extract, and the raw material components comprise:
10 parts of lactobacillus rhamnosus XM 106;
35 parts by weight of konjak extract;
1 part by weight of pyrroloquinoline quinone;
and 69 parts by weight of xylo-oligosaccharide.
Wherein the konjak extract is prepared by the following method:
(1) freeze drying rhizoma Amorphophalli at-25 deg.C, pulverizing, sieving to obtain 40 mesh fine powder, soaking in 3 times of water, and heating at 70 deg.C for 50 min;
(2) cooling the konjac subjected to the heating treatment in the step (1) to 55 ℃, adding neutral protease accounting for 0.065% of the dry weight of the konjac (the mass of the konjac fine powder obtained after the sieving treatment in the step 1) and having an enzyme activity of 30 ten thousand IU at 55 ℃ for carrying out first enzymolysis for 6 hours, adjusting the pH value to 2 to be acidic, adding pepsin accounting for 0.065% of the dry weight of the konjac (the mass of the konjac fine powder obtained after the sieving treatment in the step 1) and having an enzyme activity of 12.5 ten thousand IU for carrying out second enzymolysis for 18 hours, adjusting the pH value to 7 to be neutral by using a sodium bicarbonate solution, and filtering to obtain a hydrolysis extract;
(3) freeze-drying the hydrolysis extract at-40 deg.C, and vacuum drying at 30 deg.C for 3.5h to obtain rhizoma Amorphophalli extract.
The composition containing the probiotics and the konjak extract is prepared by the following method:
weighing the raw material components, and fully and uniformly mixing to obtain the composition containing the probiotics and the konjak extract.
Example 5
The embodiment provides a composition containing probiotics and a konjak extract, and the raw material components comprise:
30 parts of lactobacillus rhamnosus XM 106;
20 parts of konjak extract;
2 parts of pyrroloquinoline quinone;
and 33 parts of xylo-oligosaccharide.
Wherein the konjak extract is prepared by the following method:
(1) freeze drying rhizoma Amorphophalli at-30 deg.C, pulverizing, sieving to obtain 40 mesh fine powder, soaking in 3 times of water, and heating at 60 deg.C for 60 min;
(2) cooling the konjac subjected to the heating treatment in the step (1) to 55 ℃, adding neutral protease accounting for 0.06% of the dry weight of the konjac (the mass of the konjac fine powder obtained after the sieving treatment in the step 1) and having an enzyme activity of 30 ten thousand IU for carrying out first enzymolysis for 6 hours at 55 ℃, adjusting the pH value to 2.5 to be acidic, adding pepsin accounting for 0.06% of the dry weight of the konjac (the mass of the konjac fine powder obtained after the sieving treatment in the step 1) and having an enzyme activity of 12 ten thousand IU for carrying out second enzymolysis for 16 hours, adjusting the pH value to 7 to be neutral by using a sodium bicarbonate solution, and filtering to obtain a hydrolysis extract;
(3) freeze-drying the hydrolysis extract at-45 deg.C, and vacuum-drying at 30 deg.C for 3.5h to obtain rhizoma Amorphophalli extract.
The composition containing the probiotics and the konjak extract is prepared by the following method:
weighing the raw material components, and fully and uniformly mixing to obtain the composition containing the probiotics and the konjak extract.
The recommended consumption amount of the composition containing the probiotics and the konjak extract is 5g/60kg per day for human bodies.
Examples of the experiments
(1) An animal experiment for detecting the function of the composition for regulating the intestinal flora in embodiment 3 of the invention specifically comprises the following steps:
60 healthy Kunming mice are selected as experimental objects and divided into four groups, and a low dose group, a medium dose group and a high dose group are set, wherein the three dose groups are respectively 0.5 g/kg, 0.8 g/kg and 1.2g/kg, and a normal saline control group is additionally arranged.
The changes of intestinal microorganisms of the mice before and after feeding are detected and are shown in the table 1 and the table 2.
TABLE 1 Effect of the compositions on Lactobacillus plantarum at different additive amounts
| Group of | Number of animals | Before sample (CFU/g) | After sample preparation (CFU/g) |
| Control group | 15 | 1.23×108 | 1.10×108 |
| 0.5g/kg | 15 | 1.15×108 | 1.70×108 |
| 0.8g/kg | 15 | 1.33×108 | 1.95×108 |
| 1.2g/kg | 15 | 1.28×108 | 2.18×108 |
TABLE 2 Effect of the compositions on enterococcus in animals at different additive amounts
| Group of | Number of animals | Before sample (CFU/g) | After sample feeding(CFU/g) |
| Control group | 15 | 2.23×104 | 2.35×104 |
| 0.5g/kg | 15 | 2.52×104 | 2.17×104 |
| 0.8g/kg | 15 | 1.98×104 | 2.16×104 |
| 1.2g/kg | 15 | 2.09×104 | 1.99×104 |
As can be seen from tables 1-2, the composition can obviously increase the number of lactobacillus in the intestinal tract of mice and has obvious influence on enterococcus, which indicates that the composition has the function of regulating the intestinal flora.
(2) An animal experiment for detecting the sleep improvement function of the composition of embodiment 3 of the invention specifically comprises the following steps:
60 healthy Kunming mice are selected as experimental objects and divided into four groups, and a low dose group, a medium dose group and a high dose group are set, wherein the three dose groups are respectively 0.5 g/kg, 0.8 g/kg and 1.2g/kg, and a normal saline control group is additionally arranged.
And detecting the change conditions of prolonging the sleep time of the pentobarbital sodium, hypnotizing the dose under the pentobarbital sodium valve and the sleep latency of the pentobarbital sodium by different additive doses. See tables 3-5 for details.
TABLE 3 Effect of sub-threshold dose of sodium pentobarbital on inducing sleep time in mice
| Group of | Number of animals | Time to sleep second | Sleep time second |
| Control group | 15 | 260±102 | 2155±430 |
| 0.5g/kg | 15 | 233±180 | 2230±640 |
| 0.8g/kg | 15 | 205±130 | 2385±510 |
| 1.2g/kg | 15 | 215±140 | 2195±670 |
TABLE 4-Effect of sub-threshold dose of sodium pentobarbital on the incidence of sleep in mice
TABLE 5 Effect of sodium pentobarbital on sleep latency in animals
| Group of | Number of animals | Sleep latency (second) |
| Control group | 15 | 1895±430 |
| 0.5g/kg | 15 | 1810±350 |
| 0.8g/kg | 15 | 1678±510 |
| 1.2g/kg | 15 | 1458±230 |
As can be seen from tables 3-5, the dose of pentobarbital sodium under the valve of the mouse in the groups with different additive doses of the composition of the invention induces the sleep time and sleep time of the mouse under sleep, the number of animals falling asleep and the sleep latency period to be obviously different from those of the control group. This indicates that the sample has a sleep improving function.
The experimental results show that the composition has the functions of regulating intestinal flora and improving sleep.
The present invention is not limited to the above-mentioned preferred embodiments, and any other products in various forms can be obtained by anyone in the light of the present invention, but any changes in the shape or structure thereof, which have the same or similar technical solutions as those of the present application, fall within the protection scope of the present invention.
Claims (10)
1. A composition containing probiotics and konjak extract is characterized in that raw material components comprise lactobacillus rhamnosus, konjak extract and vitamin coenzyme.
2. The composition of claim 1, wherein the feedstock components comprise:
10-30 parts of lactobacillus rhamnosus;
20-35 parts by weight of konjak extract;
1-2 parts of vitamin coenzyme.
3. The composition of claim 1, wherein the feedstock components comprise:
20 parts of lactobacillus rhamnosus;
28 parts of konjak extract;
1.5 parts of vitamin coenzyme.
4. The composition of claim 1, further comprising 33-69 parts by weight of a food adjuvant.
5. The composition of claim 1, wherein the lactobacillus rhamnosus is lactobacillus rhamnosus XM 106.
6. The composition of claim 1, wherein the vitamin-coenzyme is pyrroloquinoline quinone.
7. The composition as claimed in claim 1, wherein the konjak extract is prepared by the following method:
(1) freeze drying rhizoma Amorphophalli, pulverizing, sieving, soaking in water, and heating;
(2) cooling the konjac subjected to the heating treatment in the step (1), adding neutral protease for first enzymolysis, adjusting the pH value to acidity, adding pepsin for second enzymolysis, adjusting the pH value to neutrality, and filtering to obtain a hydrolysis extract;
(3) and drying the hydrolysis extracting solution to obtain the konjak extract.
8. The composition comprising probiotics and konjak extract according to claim 7, wherein in the step (1), the freeze-drying is carried out at a temperature of-30 to-25 ℃ for 2 to 5 hours;
the particle size after crushing and sieving is 30-50 meshes, the temperature for carrying out the heating treatment is 55-75 ℃, and the time for carrying out the heating treatment is 45-90 min;
in the step (2), the temperature is reduced to 50-60 ℃;
the enzyme activity of the neutral protease is 20-40 ten thousand IU, the addition amount of the neutral protease accounts for 0.05-0.08% of the dry weight of the konjak, the temperature for carrying out the first enzymolysis is 50-60 ℃, and the time for carrying out the first enzymolysis is 4-8 h; the enzyme activity of the pepsin is 5-20 ten thousand IU, the addition amount of the pepsin accounts for 0.05-0.08% of the dry weight of the konjak, the enzymolysis temperature is 40-50 ℃, and the enzymolysis time is 12-24 hours;
in the step (3), the drying is to freeze and dry the extracting solution firstly and then to carry out vacuum drying.
9. The method for preparing a composition comprising a probiotic and an extract of konjak according to any one of claims 1 to 8, comprising the steps of:
weighing the raw material components, and fully and uniformly mixing to obtain the composition containing the probiotics and the konjak extract.
10. Use of a composition according to any one of claims 1 to 8 in a health product for regulating the intestinal flora and improving sleep.
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| WO2022042293A1 (en) * | 2020-08-25 | 2022-03-03 | 浙江李子园食品股份有限公司 | Method for preparing reproduced konjac granules |
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| CN106619743A (en) * | 2016-12-28 | 2017-05-10 | 山东龙力生物科技股份有限公司 | Probiotic solid drink with hypoglycaemic effect and preparation method of probiotic solid drink |
| CN110179121A (en) * | 2019-05-13 | 2019-08-30 | 湖南唯乐可健康产业有限公司 | It is a kind of to improve the 5-linked probiotic composition of enteron aisle, compound solid beverage and its preparation method and application |
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| CN103436582A (en) * | 2013-07-10 | 2013-12-11 | 马杰坤 | Konjac polypeptide extract, as well as preparation method and application thereof |
| CN106619743A (en) * | 2016-12-28 | 2017-05-10 | 山东龙力生物科技股份有限公司 | Probiotic solid drink with hypoglycaemic effect and preparation method of probiotic solid drink |
| CN110179121A (en) * | 2019-05-13 | 2019-08-30 | 湖南唯乐可健康产业有限公司 | It is a kind of to improve the 5-linked probiotic composition of enteron aisle, compound solid beverage and its preparation method and application |
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