CN110915809A - Preparation method of disinfectant capable of killing brucella - Google Patents

Preparation method of disinfectant capable of killing brucella Download PDF

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CN110915809A
CN110915809A CN201911237784.9A CN201911237784A CN110915809A CN 110915809 A CN110915809 A CN 110915809A CN 201911237784 A CN201911237784 A CN 201911237784A CN 110915809 A CN110915809 A CN 110915809A
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disinfectant
aminopropyl
bis
brucella
killing
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李传碧
韩爽
刘春玲
张首才
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Jilin Normal University
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Jilin Normal University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
    • A01N33/02—Amines; Quaternary ammonium compounds
    • A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/13—Amines
    • A61K31/131—Amines acyclic
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/13—Amines
    • A61K31/155—Amidines (), e.g. guanidine (H2N—C(=NH)—NH2), isourea (N=C(OH)—NH2), isothiourea (—N=C(SH)—NH2)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Dentistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Epidemiology (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Environmental Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Molecular Biology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a preparation method of a disinfectant capable of killing brucella. Firstly, adding a certain amount of solvent into an enamel kettle, heating the solvent to 20-40 ℃, adding the polyguanidine compound and the bis (3-aminopropyl) alkylamine compound in proportion, continuing stirring for 5-30 minutes after the addition is finished, adjusting the pH value to 4.5-7 by using 15-30% hydrochloric acid, and stirring for 5-15 minutes to obtain the disinfectant. The invention takes polymethylene guanidine hydrochloride and bis (3-aminopropyl) alkylamine as main components and water as a solvent, and the prepared disinfectant can be applied to disinfection of various livestock breeding places. Especially has high killing capacity to Brucella, has good killing effect to hepatitis B virus, spore, fungus and germ, and has obvious treatment effect to foot-and-mouth disease, onychomycosis, collunarium and the like.

Description

Preparation method of disinfectant capable of killing brucella
Technical Field
The invention relates to a disinfectant for animals or medical use, in particular to a preparation method of a disinfectant capable of killing brucella.
Background
Modern chemical disinfectants have been developed rapidly and widely used since the advent, and at present, there are roughly several major classes, namely acids, alcohols, halogens, gaseous volatile alkanes, and the like. The disinfectants have the common defects of poor stability, high corrosiveness and strong irritation to human bodies, and some of the disinfectants have certain toxicity and are harmful to human and livestock. The quaternary ammonium salt disinfectant which appears in recent years has wide application, but has poor quick killing capability, poor organic compound interference resistance and higher cost. Brucellosis, also known as flexblet, is a zoonosis caused by brucellosis. The previous special disinfectant for brucella is lysol, which is prohibited by the nation, and many disinfectant products in the market at present, such as 84 disinfectant, peroxyacetic acid and the like, can kill brucella. However, the special bactericide for the brucella has few types and poor killing effect, and no specific disinfectant exists at present.
Disclosure of Invention
The invention aims to provide a preparation method of a disinfectant capable of killing brucella, the disinfectant can be used for sterilization and disinfection in public places, the disinfection and bactericidal power is higher than that of the existing disinfectant, and the disinfectant especially has very high killing capability on the brucella.
Technical scheme of the invention
A method for preparing a disinfectant capable of killing Brucella comprises the steps of firstly adding a certain amount of solvent into an enamel kettle, heating the solvent to 20-40 degrees, adding a polyguanidine compound and a bis (3-aminopropyl) alkylamine compound in proportion, continuing stirring for 5-30 minutes after the addition is finished, adjusting the pH value to 4.5-7 by using 15-30% hydrochloric acid, and stirring for 5-15 minutes to obtain the disinfectant.
The polyguanidine compound is guanidine polymer containing 4-6 methylene groups, the polymerization degree n is more than or equal to 10, and the structural formula is shown in the specification
Figure BDA0002304433300000011
Wherein m is more than or equal to 4 and less than or equal to 6, and n is more than or equal to 10.
The structural formula of the bis (3-aminopropyl) alkylamine is shown in the following formula, wherein R is alkyl with 8-12 carbon atoms
Figure BDA0002304433300000021
The content of the polyguanidine compound is 5-35 parts by weight, and the content of the bis (3-aminopropyl) alkylamine compound is 1-15 parts by weight.
The optimal weight ratio of the polyguanidine compound to the bis (3 aminopropyl) alkylamine is 2: 1.
the polyguanidine compound is polyhexamethylene guanidine hydrochloride, the bis (3-aminopropyl) alkylamine compound is bis (3-aminopropyl) dodecylamine, the solvent is water, and the mass ratio of the polyhexamethylene guanidine hydrochloride to the bis (3-aminopropyl) dodecylamine to the water is 2: 1.5: 96.5.
the invention has the advantages of
The invention takes polymethylene guanidine hydrochloride and bis (3-aminopropyl) alkylamine as main components and water as a solvent, and the prepared disinfectant can be applied to disinfection of various livestock breeding places. The disinfection and sterilization capacity is higher than that of the existing disinfectant. Meanwhile, the disinfectant has the characteristics of high efficiency, broad spectrum, no color, no odor, no toxicity, no stimulation, no residue, no corrosion, strong bactericidal power and the like, and can be widely applied to the sterilization and disinfection of other public places. Especially has high killing capacity to Brucella, has good killing effect to hepatitis B virus, spore, fungus and germ, and has obvious treatment effect to foot-and-mouth disease, onychomycosis, collunarium and the like.
Drawings
FIG. 1 is a graph comparing the bactericidal effect of polyhexamethylene guanidine hydrochloride, bis (3-aminopropyl) alkylamine and the disinfectant of the present application on Brucella.
Detailed Description
The applicant invented the present application against the problem in the background art, and against the brucella disinfectant.
A method for preparing a disinfectant capable of killing Brucella comprises the steps of firstly adding a certain amount of solvent into an enamel kettle, heating the solvent to 20-40 degrees, adding a polyguanidine compound and a bis (3-aminopropyl) alkylamine compound in proportion, continuing stirring for 5-30 minutes after the addition is finished, adjusting the pH value to 4.5-7 by using 15-30% hydrochloric acid, and stirring for 5-15 minutes to obtain the disinfectant.
The polyguanidine compound is guanidine polymer containing 4-6 methylene groups, the polymerization degree n is more than or equal to 10, and the structural formula is shown in the specification
Figure BDA0002304433300000022
Wherein m is more than or equal to 4 and less than or equal to 6, and n is more than or equal to 10.
The structural formula of the bis (3-aminopropyl) alkylamine is shown in the following formula, wherein R is alkyl with 8-12 carbon atoms
Figure BDA0002304433300000023
The content of the polyguanidine compound is 5-35 parts by weight, and the content of the bis (3-aminopropyl) alkylamine compound is 1-15 parts by weight.
The optimal weight mixing ratio of the polyguanidine compound to the bis (3 aminopropyl) alkylamine is 2: 1.
the polyguanidine compound is polyhexamethylene guanidine hydrochloride, the bis (3-aminopropyl) alkylamine compound is bis (3-aminopropyl) dodecylamine, the solvent is water, and the mass ratio of the polyhexamethylene guanidine hydrochloride to the bis (3-aminopropyl) dodecylamine to the water is 2: 1.5: 96.5.
when in use, the composite disinfectant is diluted by water or ethanol solvent.
Example 1:
firstly, 35000kg of medical deionized water is added into an enamel kettle, the solvent is heated to 40 ℃, 200kg of polyguanidine compound and 150kg of bis (3-aminopropyl) alkylamine compound are added according to the proportion, stirring is continued for 30 minutes after the addition is finished, and the pH value is adjusted to 4.5-7, preferably 5 by 30% medical hydrochloric acid. And stirring for 15 minutes to obtain the disinfectant.
Example 2:
firstly, 40000kg of medical deionized water is added into an enamel kettle, the solvent is heated to 20 ℃, 210kg of polyguanidine compound and 140kg of bis (3-aminopropyl) alkylamine compound are added according to the proportion, the mixture is continuously stirred for 5 minutes after the addition is finished, and the pH value is adjusted to 4.5 by 15 percent of medical hydrochloric acid. And stirring for 10 minutes to obtain the disinfectant.
Example 3:
firstly, 36000 kg of medical deionized water is added into an enamel kettle, the solvent is heated to 30 ℃, 220kg of polyhexamethylene hydrochloride guanidine compounds and 130kg of bis (3-aminopropyl) alkylamine compounds are added according to the proportion, the stirring is continued for 20 minutes after the addition is finished, and the pH value is adjusted to 7 by using 35% medical hydrochloric acid. And stirring for 5 minutes to obtain the disinfectant.
Example 4:
firstly, 96.5 kg of medical deionized water is added into an enamel kettle, the solvent is heated to 30 ℃, 2kg of polyhexamethylene guanidine hydrochloride and 1.5kg of bis (3-aminopropyl) dodecylamine are added according to the proportion, the stirring is continued for 20 minutes after the addition is finished, and the pH value is adjusted to 5 by using 35% medical hydrochloric acid. And stirring for 15 minutes to obtain the disinfectant.
Experiment:
the composite disinfectant synthesized by the application is prepared into a 1% solution (experiment medicine three) to be compared with a 1% polyhexamethylene guanidine hydrochloride (experiment medicine two) and a 1% bis (3-aminopropyl) alkylamine (experiment medicine one) disinfectant sterilization experiment.
Experimental drugs: experiment medicine one, experiment medicine two and experiment medicine three
Experimental strains: brucella
The experimental process comprises the following steps:
1. weighing the three medicines, weighing the three medicines respectively, adding water to dissolve the three medicines, and preparing the experiment after the three medicines are completely dissolved.
2. Taking the culture medium for the experimental strains to perform the following steps of 1: 1000 and three 96-well plates were simultaneously taken. The diluted bacterial liquid is added into the front seven rows of 96-well plates, the addition amount of each row of each well is 150 microliters, and three 96-well plates are added in total.
3. 150 microliters of diluted bacteria liquid and 30 microliters of drugs are sequentially added into the first three rows of one hole of each 96-plate.
The dilution was carried out sequentially backwards using a calandria, for a total of 24 concentration gradients. The seventh row of the well plate is a control of blank bacteria liquid.
4. 150 microliters of medium was added to each eighth row of the well plate as a blank.
5. After all the reagents are added, the 96-well plate is sealed by a sealing film, the information of the three drugs is respectively marked, and the three drugs are put into an incubator at 37 ℃ for culture.
6. After culturing for 2h, 4h, 6h, 8h and 10h, detecting by using a microplate reader and recording the bacteria liquid data (the detection wavelength is 630 nm).
From fig. 1 it can be seen that the leftmost 1% bis (3-aminopropyl) alkylamine (mono) disinfectant loses bacteriostatic activity upon 8-fold dilution; the 1% polyhexamethylene guanidine hydrochloride (II) series 2 middle curve shows that the disinfectant loses antibacterial activity when diluted by 12 times, and the antibacterial effect is better than that of a bis (3-aminopropyl) alkylamine (I) disinfectant with the same concentration, but the antibacterial and antibacterial activity of the most right side of the 1% composite disinfectant (III) with the same concentration is best, and the disinfectant still has the antibacterial and antibacterial activity when diluted by 19 times (so that the supposedly strong antibacterial effect exists when the concentration of the composite disinfectant is 0.5%).

Claims (6)

1. A preparation method of a disinfectant capable of killing Brucella is characterized by comprising the following steps:
firstly, adding a certain amount of solvent into an enamel kettle, heating the solvent to 20-40 ℃, adding the polyguanidine compound and the bis (3-aminopropyl) alkylamine compound in proportion, continuing stirring for 5-30 minutes after the addition is finished, adjusting the pH value to 4.5-7 by using 15-30% hydrochloric acid, and stirring for 5-15 minutes to obtain the disinfectant.
2. The method for preparing a disinfectant for killing brucella as claimed in claim 1, wherein: the polyguanidine compound is guanidine polymer containing 4-6 methylene groups, the polymerization degree n is more than or equal to 10, and the structural formula is shown in the specification
Figure FDA0002304433290000011
Wherein m is more than or equal to 4 and less than or equal to 6, and n is more than or equal to 10.
3. The method for preparing a disinfectant for killing brucella as claimed in claim 1, wherein: the structural formula of the bis (3-aminopropyl) alkylamine is shown in the following formula, wherein R is alkyl with 8-12 carbon atoms
Figure FDA0002304433290000012
4. The method for preparing a disinfectant for killing brucella as claimed in claim 1, wherein: the content of the polyguanidine compound is 5-35 parts by weight, and the content of the bis (3-aminopropyl) alkylamine compound is 1-15 parts by weight.
5. The method for preparing a disinfectant for killing brucella as claimed in claim 1, wherein: the optimal weight ratio of the polyguanidine compound to the bis (3 aminopropyl) alkylamine is 2: 1.
6. the method for preparing a disinfectant for killing brucella as claimed in claim 1, wherein: the polyguanidine compound is polyhexamethylene guanidine hydrochloride, the bis (3-aminopropyl) alkylamine compound is bis (3-aminopropyl) dodecylamine, the solvent is water, and the mass ratio of the polyhexamethylene guanidine hydrochloride to the bis (3-aminopropyl) dodecylamine to the water is 2: 1.5: 96.5.
CN201911237784.9A 2019-12-05 2019-12-05 Preparation method of disinfectant capable of killing brucella Pending CN110915809A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2006122738A (en) * 2006-06-27 2008-01-10 Общество с ограниченной ответственностью "Международный институт эколого-технологических проблем" (RU) METHOD FOR PRODUCING BIOCIDE POLIGUANIDINE AND BIOCIDE POLIGUANIDINE
RU2317950C1 (en) * 2006-07-17 2008-02-27 Общество с ограниченной ответственностью "Международный институт эколого-технологических проблем" Disinfecting agent
CN101578045A (en) * 2006-12-26 2009-11-11 斯特里莱克斯科技有限责任公司 Antimicrobial compositions
RU2632461C1 (en) * 2016-07-15 2017-10-05 Региональная общественная организация - Институт эколого-технологических проблем (РОО ИЭТП) Disinfectant
CN108739855A (en) * 2018-06-05 2018-11-06 陈琪峰 A kind of mthod of white water from paper making fungicide and preparation method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2006122738A (en) * 2006-06-27 2008-01-10 Общество с ограниченной ответственностью "Международный институт эколого-технологических проблем" (RU) METHOD FOR PRODUCING BIOCIDE POLIGUANIDINE AND BIOCIDE POLIGUANIDINE
RU2324478C2 (en) * 2006-06-27 2008-05-20 Общество с ограниченной ответственностью "Международный институт эколого-технологических проблем" Method of biocide polyguanidine production and biocide polyguanidine
RU2317950C1 (en) * 2006-07-17 2008-02-27 Общество с ограниченной ответственностью "Международный институт эколого-технологических проблем" Disinfecting agent
CN101578045A (en) * 2006-12-26 2009-11-11 斯特里莱克斯科技有限责任公司 Antimicrobial compositions
RU2632461C1 (en) * 2016-07-15 2017-10-05 Региональная общественная организация - Институт эколого-технологических проблем (РОО ИЭТП) Disinfectant
CN108739855A (en) * 2018-06-05 2018-11-06 陈琪峰 A kind of mthod of white water from paper making fungicide and preparation method thereof

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Application publication date: 20200327