CN110915726A - Method and device for collecting live bee venom and detecting weight of bee venom - Google Patents
Method and device for collecting live bee venom and detecting weight of bee venom Download PDFInfo
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- CN110915726A CN110915726A CN201911362611.XA CN201911362611A CN110915726A CN 110915726 A CN110915726 A CN 110915726A CN 201911362611 A CN201911362611 A CN 201911362611A CN 110915726 A CN110915726 A CN 110915726A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K59/00—Honey collection
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
Abstract
The invention discloses a live bee venom collection method, which comprises the following steps: s1, preparing a gel plate with the pH value of 4.5-7.0, wherein a hydrophobic elastic film is arranged on the surface of gel in the gel plate; s2 pricking the gel plate with alive bee sting, and keeping the bee needle in the gel via hydrophobic elastic membrane, and continuously injecting bee venom; s3 after bee venom injection, removing the hydrophobic elastic membrane and bee needle thereon, and sealing the gel plate for storage. The invention also discloses a method and a device for detecting the bee venom weight of the live bees. Compared with the prior art, the bee venom which is obtained by sealing the bee stings after the bee stings and stings by using the gel can be used for detecting the weight of the bee venom on one hand, and can be directly used as a corresponding processing raw material of the bee venom product together with the gel on the other hand, and the weight of the bee venom in the gel is detected by using a weight detection method when the bee venom is used. By arranging the hydrophobic elastic membrane on the surface of the gel, the bee needles of the live bees can be actively left in the gel plate and continuously injected into bee venom, so that the operation is convenient, and the collection of the bee venom is more complete.
Description
Technical Field
The invention belongs to the field of bee venom collection and detection, and particularly relates to a method and a device for bee venom collection and bee venom weight detection.
Background
The bee venom is clinically applied to treating rheumatoid arthritis, atrophic arthritis, malformed arthritis and other polyarthritis, has certain curative effect, and the curative effect is gradually obvious with the passage of treatment time. Bee venom is also applied to the treatment of various inflammations in ophthalmology, stomatology, dermatology and the like, can effectively inhibit the growth of tumors, and has obvious effect.
Bee venom is venom in bee venom sac, and is discharged out of body through a chela needle when bee stings. The bees researched at present mainly comprise Chinese bees, Italian bees, Canniella bees, European black bees and the like, and bee venom of the bees is a very complex mixture of components, and mainly comprises active enzymes, polypeptides, amines, saccharides, esters, amino acids and a small amount of choline and volatile compounds (pheromone).
The existing bee venom collection method is to keep the bee venom after stinging on a glass plate, and collect the powder after drying in the shade. However, the ingredients of bee venom are very complex, and in the process of drying in the shade, partial substances in the bee venom are volatilized and degraded, so that partial effective substances are lost, so that the clinical effect of the bee venom products produced by the current pharmaceutical factories is not ideal after use, and the bee venom products are far from being used for stinging by live bees. At present, no better collection mode of live bee venom and a method for detecting the weight of the bee venom exist at home and abroad, the application of the bee venom is severely restricted, and the development of industries such as live bee treatment, bee venom analysis research, production of related derivatives and the like is limited.
For live bees, different bee species, different breeding environments, different feeding methods, even by feeding sugar water containing medicinal components (such as Chinese herbal decoction), can affect the bee venom amount and bee venom activity of the live bees, so that there is a difference in bee venom weight of average individuals of the same age of day among live bees of different populations.
The existing production method of bee venom products, especially the method for producing liquid medicine or suppository by using powdery bee venom raw materials, loses part of effective substances in the powder preparation process, and the component proportion of the product is calculated by the powder, so that the bee venom product has poor effect. Even if the stability of products produced in different batches is influenced to a certain extent by the same formula, the effect of the final bee venom product can be influenced by different production processes or manufacturers and different quality control or factory detection standards. Some liquid bee venom products prepared by the existing manufacturers, even bee venom with the same volume, have great difference in the content and activity of bee venom. The volume of bee venom product produced by manufacturers can be known when the bee venom product is extracted by using a syringe, but the moisture in the bee venom is extremely volatile, so that the weight of the bee venom product is difficult to be objectively measured.
The method is characterized by completely collecting the live bee venom and screening the bees with stable quality through weight detection, and is favorable for improving the effect and the stability of related products in the bee venom product industry. However, there is no effective solution in the prior art.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a method for collecting live bee venom, which has the advantages of comprehensive bee venom substance preservation, low cost, simple operation, capability of directly detecting the weight of the bee venom on the basis of the method and high result accuracy, and is realized by the following technical scheme:
a live bee venom collection method comprises the following steps:
s1, preparing a gel plate with the pH value of 4.5-7.0, wherein a hydrophobic elastic film is arranged on the surface of gel in the gel plate;
s2 pricking the gel plate with alive bee sting, and keeping the bee needle in the gel via hydrophobic elastic membrane, and continuously injecting bee venom;
s3 after bee venom injection, removing the hydrophobic elastic membrane and bee needle thereon, and sealing the gel plate for storage.
Optionally, in step S1, the gel plate may be an agar plate, a medical gelatin plate, or a solid collagen egg white plate. The collected bee venom can be directly stored for producing related products, and can be used without peeling the bee venom out. For example, the solid collagen plate can be directly used in the formula of the bee venom facial mask, or the solid collagen plate is made of human absorbable collagen, such as solid collagen made of catgut material. In addition, the gel can be used together with venenum Apis for grinding and making into suppository.
Optionally, in the step S1, the solid gel in the gel plate is selected from a gel that turns into a liquid state when heated to 42-60 ℃.
Optionally, the live bee venom collection method further comprises a step S4 of heating the gel in the gel plate to 42-60 ℃, and sealing and storing the gel after the gel is converted from a solid state to a liquid state. The liquid bee venom-containing gel can be directly used as raw material or processed into suppository.
Optionally, in step S1, the hydrophobic elastic membrane is wetted by double distilled water or deionized water, and can be tightly attached to the surface of the gel plate.
Alternatively, in step S2, a greater amount of bee venom can be obtained by using multiple bees to sting in the same gel plate at a time.
Optionally, in step S2, the bee venom is continuously injected into the bee needles for 15-35 min. Because the venom sac glands at the tail ends of the bee needles after bee sting can continuously move for a certain time, bee venom in the bee venom sacs is continuously injected into the gel plate, the bee needles are pulled out after staying for 15-35 min in the gel plate, and the obtained bee venom is more and more complete.
Optionally, in step S2, the bee venom is continuously injected into the bee needles for 20-30 min. Bee venom is basically injected after 20min, the injected bee venom amount is less after 30min, the influence on the whole weight of bee venom is less, the activity of bee needles is basically lost, and bee venom in bee sacs cannot be injected continuously.
Compared with the prior art, the live bee venom collection method provided by the invention has the advantages that the gel is used for sealing and storing the bee venom after the live bee sting is stinged, on one hand, the gel can be used for bee venom weight detection, on the other hand, the gel and the live bee venom can be directly used as corresponding bee venom product processing raw materials together, and in addition, the weight of the bee venom in the gel is detected and obtained through a weight detection method during use. By arranging the hydrophobic elastic membrane on the surface of the gel, the bee needles of the live bees can be actively left in the gel plate and continuously injected into bee venom, so that the operation is convenient, and the collection of the bee venom is more complete. The pH value of the gel plate and the pH value of the bee venom are in the same range, so that the degradation rate of substances in the bee venom is reduced, and the storage time of active substances in the bee venom is prolonged.
The invention also provides a method for detecting the weight of live bee venom, which can comprehensively, conveniently and quickly retain bee venom substances and can be directly used as a raw material for producing bee venom products after weight detection, and the method is realized by the following technical scheme:
a method for detecting the weight of live bee venom comprises the following steps:
s10 preparing a gel plate with a pH value of 4.5-7.0, and weighing the gel plate to obtain m0The surface of the gel in the gel plate is provided with a hydrophobic elastic membrane;
s20 pricking the gel plate with alive bee sting, and keeping the bee needle in the gel via hydrophobic elastic membrane, and continuously injecting bee venom;
s30 removing the hydrophobic elastic membrane and the bee needle, weighing the gel plate to m1Sealing and storing the gel plate;
s40 calculating bee venom weight m2Is m1-m0。
Alternatively, in step S20, a plurality of bees can be respectively stunted in the same gel plate at a time to obtain a larger amount of bee venom. The accumulation of a plurality of bee venom increases the specific gravity of the bee venom relative to the gel plate, reduces the requirement of a balance, and enlarges the application range.
Optionally, in step S20, the one or more of the selected bee population, the working age, the feeding conditions and the feeding drugs that are stinging in the same gel plate are the same or similar. The total weight of the bee venom of the bees under the limited condition can be counted conveniently, and the average weight of the bee venom of each bee can be calculated according to the number of the bees.
Optionally, in step S10, the gel plate may be an agar plate, a medical gelatin plate, or a solid collagen egg white plate. The collected bee venom can be directly stored for producing related products after weighing, and can be used without peeling the bee venom out. For example, the solid collagen egg white plate can be directly used in the formula of the bee venom facial mask.
Optionally, in the step S10, the solid gel in the gel plate is selected from a gel that turns into a liquid state when heated to 42-60 ℃.
Optionally, in step S30, the sealed gel plate is sealed and stored when heated to be liquid gel.
Optionally, in step S10, the hydrophobic elastic membrane is wetted by double distilled water or deionized water, and can be tightly attached to the surface of the gel plate.
Alternatively, in step S20, a greater amount of bee venom can be obtained by using multiple bees to sting in the same gel plate at a time.
Optionally, in step S20, the bee venom is continuously injected into the bee needles for 15-35 min.
Optionally, in step S20, the bee venom is continuously injected into the bee needles for 20-30 min.
Optionally, the method further includes step S50, when the number of bees in step S20 is n, the average weight of each bee venom is m, and the total amount of the bee venom obtained in step S40 is obtained when a plurality of bees are respectively stunted in the same gel plate to obtain a larger amount of bee venom2/n。
Compared with the prior art, the live bee venom weight detection method provided by the invention has the advantages that the bee venom after the live bee sting is sealed and stored by the gel, the bee venom can be stored more completely and can be stored in the gel plate by the bee venom, and the bee venom weight data is obtained by weighing the mass change before and after the gel plate. In addition, the gel is used for sealing and storing the bee venom which is stinging by the alive bee, and the bee venom can be directly used as a production raw material of a bee venom product after weighing is finished.
The invention also discloses a device which has comprehensive bee venom substance collection, low cost and simple operation, can directly detect the weight of bee venom on the basis of the method and has high result accuracy, and the device is realized by the following technical scheme:
the bee venom collecting and weight detecting device comprises a gel plate, wherein the pH value of the gel plate is 4.5-7.0, and a hydrophobic elastic membrane is arranged on the surface of the gel.
Optionally, the hydrophobic elastic membrane is a rubber membrane or a latex membrane, and other membranes capable of sealing the gel surface and separating the bee needle from the body can be replaced.
Optionally, the hydrophobic elastic membrane is a single layer or multiple layers.
Optionally, the hydrophobic elastic membrane is wetted by double distilled water or deionized water and attached to the surface of the gel. The interfacial tension of make full use of water for when living bee stings the thorn, the barb structure on bee needle surface can be blocked to the hydrophobic membrane of elasticity, makes bee needle and sealed body separation, stays in the agar-agar plate.
Optionally, the gel plate is one of an agar plate, a medical gelatin plate or a solid collagen egg white plate.
Optionally, the pH value of the gel plate is 4.5-7.0.
Optionally, the gel plate contains chloramphenicol, so that on one hand, growth of microorganisms is effectively inhibited, the service life of the gel plate and the shelf life of the gel plate filled with bee venom are prolonged, on the other hand, the use range of the gel plate is expanded, and the gel plate can be used and stored in a non-strict laboratory environment.
Optionally, the diameter of the gel plate is 2-8 cm. The gel plates have different diameters and are suitable for different detection objects. Such as: a 2cm diameter gel plate suitable for measuring the weight of 1 live bee or 1 needle of bee venom sample; gel plates of 8cm diameter, suitable for simultaneous detection of the weight of 10 live bees or 10 needles of bee venom samples.
When in use, bee venom is injected into the gel of the gel plate via micro-injector or live bee sting. The weight of bee venom can be detected or substances in the gel plate can be materialized or replaced as required to realize other functions, for example, the gel plate is an agar plate containing red blood cells, after bee venom is injected, a hemolytic ring is generated, and after incubation for a period of time, the hemolytic activity of the bee venom can be reflected by observing and comparing the size of the hemolytic ring.
Compared with the prior art, the bee venom detection device can completely collect live bee venom by using the gel plate, so that the situation that bee venom components are volatilized or degraded is avoided; the device can be used for detecting the weight and activity of bee venom products, and when the activity of the bee venom products is semi-quantitatively detected, the gel plate is an agar plate with red blood cells. The design of the hydrophobic elastic membrane can ensure that the humidity and the pH value of the gel plate are relatively constant, and bee needles can be left on the gel plate.
Drawings
FIG. 1 is a schematic view of the microstructure of a bee needle;
FIG. 2 is a block diagram illustrating a flow chart of a method for collecting bee venom of live bees according to an embodiment;
FIG. 3 is a block diagram illustrating a flowchart of a method for detecting bee venom weight of a live bee according to an embodiment;
fig. 4 is a schematic structural diagram of the bee venom collection and weight detection device in the embodiment.
In the figures, the reference numbers are: 1-bee and bee needle, 2-barb structure, 3-agar plate, 31-agar, 4-rubber film and 7-sealing cover.
Detailed Description
The following examples are given to illustrate specific embodiments of the present invention, but the present invention is not limited to the following examples.
Examples
The bee venom collection device shown in FIG. 4 comprises an agar plate 3, agar 31 disposed therein, a rubber membrane 4 disposed on the surface of the agar 31, and a sealing cap 7 for sealing the agar plate 3. The rubber film 4 is soaked in deionized water or double distilled water and then is tightly attached to the surface of the agar 31. The agar plate 3 is prepared by adopting agar, and chloramphenicol is added in the preparation process.
Wherein the diameter of the agar plate 3 is 2-8 cm, and the thickness of the agar is 2-6 mm.
As shown in fig. 2 and 3, the bee venom collecting and weight detecting device comprises the following steps:
s1 preparing agar plate 3 with pH value of 4.5-7.0, weighing the weight of the agar plate 3 at the moment by using an electronic balance, and marking as m0The surface of the agar 31 in the agar plate 3 is provided with a rubber film 4;
s2, the bee needle 1 is left in the agar plate 3 through the rubber film 4 by using the live bee sting to sting the agar plate 3, and bee venom is continuously injected;
s3 after bee venom injection, the weight of the agar plate 3 was measured to be m1And taking down the rubber film 4 and the bee needle 1 thereon, and sealing and storing the agar plate 3.
In the process of detecting the bee venom weight, the method further comprises the step S4 of calculating the bee venom weight m2Is m1-m0。
In the step S2, when the bee venom of the live bee is selected to be put into the agar 31 of the agar plate 3, the tail of the live bee is controlled to sting the agar 31 through the rubber film 4, because the inverted hook structure 2 arranged on the bee needle 1 is shown in figure 1, the rubber film 4 can clamp the bee needle 1, so that the bee needle 1 is separated from the bee body, at the moment, the bee needle 1 can continuously move for 15-30 min to continuously inject the bee venom in the venom sac into the agar 31, the step S3 is continued, after the injection of the bee venom is finished, the rubber film 4 and the bee needle 1 are taken down together, and the agar plate 3 is sealed and stored. The collected bee venom can be directly stored for producing related products, and can be used without peeling the bee venom out.
In step S1, the agar plate 3 is selected from the agar 31 formula which is transformed into a liquid state when heated to 42 to 60 ℃. The live bee venom collection method further comprises the step of S4, heating the agar 31 in the agar plate 3 to 42-60 ℃, and sealing and storing the agar 31 after the agar is converted from a solid state to a liquid state. The liquid agar containing bee venom can be directly used as raw material or processed into suppository.
In the process of detecting the weight of the bee venom, the method further comprises a step S5, when a plurality of bees are respectively sting in the same agar plate 3 in the step S2 to obtain a larger amount of bee venom, the total amount of the bee venom obtained in the step S4 is obtained, the number of the bees is n, and the average weight of the bee venom of each bee is m2/n。
Since the collecting method and the weight detecting method are similar in part of the steps, they are described in the same embodiment.
It should be noted that the embodiment of the present invention is only one embodiment of the core idea of the present invention, and all the substantially same schemes such as equivalent replacement or simple improvement made on the basis of the method of the present invention belong to the protection scope of the present invention.
Claims (10)
1. A live bee venom collection method is characterized by comprising the following steps: preparing a gel plate with the pH value of 4.5-7.0, wherein a hydrophobic elastic membrane is arranged on the surface of gel in the gel plate; the gel plate is punctured by using live bee sting, bee needles are remained in the gel through a hydrophobic elastic membrane, and bee venom is continuously injected into the bee needles; and after bee venom injection is completed, taking down the hydrophobic elastic membrane and the bee needles on the hydrophobic elastic membrane, and sealing and storing the gel plate.
2. The method for collecting bee venom of a live bee according to claim 1, wherein the gel plate is selected from the group consisting of an agar plate, a medical gelatin plate, and a solid collagen egg white plate.
3. The method for collecting bee venom of live bees according to claim 1, wherein the gel in the gel plate is selected from gels that turn into a liquid state when heated to 42-60 ℃.
4. The method of claim 1, wherein a plurality of bees are separately stunted in the same gel plate.
5. The method for collecting bee venom of a live bee according to claim 1, wherein the bee venom is continuously injected into the bee needle for 15-35 min.
6. A method for detecting the weight of live bee venom is characterized by comprising the following steps:
preparing a gel plate with the pH value of 4.5-7.0, and weighing the gel plate to obtain m0The surface of the gel in the gel plate is provided with a hydrophobic elastic membrane; the gel plate is punctured by using live bee sting, bee needles are left in the gel through a hydrophobic elastic membrane, and bee venom is continuously injected; after the bee venom injection is completed, the hydrophobic elastic membrane and bee needles thereon are taken down, and the weight of the gel plate is weighed to be m1Sealing and storing the gel plate; calculating to obtain bee venom weight m2Is m1-m0。
7. The method of claim 6, wherein a plurality of bees are separately stunted in the same gel plate.
8. The method of claim 7, further comprising the step of obtaining the total amount of the bee venom from a plurality of bees when they sting in the same gel sheet, respectively, wherein the average weight of the bee venom is m when the number of the bees is n2/n。
9. A bee venom collection device capable of realizing the bee venom weight detection method according to any one of claims 1 to 8, comprising: the gel plate comprises a gel plate, wherein the pH value of the gel plate is 4.5-7.0, and a hydrophobic elastic membrane is arranged on the surface of the gel.
10. The bee venom collection device according to claim 9, wherein: the gel plate is an agar plate, a medical gelatin plate or a solid collagen egg white plate.
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| CN201911362611.XA CN110915726B (en) | 2019-12-26 | 2019-12-26 | Method and device for collecting live bee venom and detecting weight of bee venom |
| CN202111202896.8A CN113826566A (en) | 2019-12-26 | 2019-12-26 | Bee venom collection system |
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| CN110915726B (en) | 2021-09-10 |
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