CN113826566A - Bee venom collection system - Google Patents

Bee venom collection system Download PDF

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Publication number
CN113826566A
CN113826566A CN202111202896.8A CN202111202896A CN113826566A CN 113826566 A CN113826566 A CN 113826566A CN 202111202896 A CN202111202896 A CN 202111202896A CN 113826566 A CN113826566 A CN 113826566A
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China
Prior art keywords
bee venom
gel
bee
plate
gel plate
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Pending
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CN202111202896.8A
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Chinese (zh)
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杨威
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Individual
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K59/00Honey collection
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56Materials from animals other than mammals
    • A61K35/63Arthropods
    • A61K35/64Insects, e.g. bees, wasps or fleas

Abstract

The invention discloses a bee venom collection device, wherein the pH value of a gel plate is 4.5-7.0, a hydrophobic elastic membrane is arranged on the surface of the gel, the hydrophobic elastic membrane is wetted by double distilled water or deionized water, and solid gel in the gel plate is selected from gel which is converted into liquid when heated to 42-60 ℃. In order to solve the problems in the prior art, the invention provides the bee venom collection device which is comprehensive in bee venom substance preservation, low in cost and simple in operation.

Description

Bee venom collection system
Technical Field
The invention relates to the technical field of bee venom collection, in particular to a bee venom collection device.
Background
The bee venom is clinically applied to treating rheumatoid arthritis, atrophic arthritis, malformed arthritis and other polyarthritis, has certain curative effect, and the curative effect is gradually obvious with the passage of treatment time. Bee venom is also applied to the treatment of various inflammations in ophthalmology, stomatology, dermatology and the like, can effectively inhibit the growth of tumors, and has obvious effect.
Bee venom is venom in bee venom sac, and is discharged out of body through a chela needle when bee stings. The bees researched at present mainly comprise Chinese bees, Italian bees, Canniella bees, European black bees and the like, and bee venom of the bees is a very complex mixture of components, and mainly comprises active enzymes, polypeptides, amines, saccharides, esters, amino acids and a small amount of choline and volatile compounds (pheromone).
The existing bee venom collection method is to keep the bee venom after stinging on a glass plate, and collect the powder after drying in the shade. However, the ingredients of bee venom are very complex, and in the process of drying in the shade, partial substances in the bee venom are volatilized and degraded, so that partial effective substances are lost, so that the clinical effect of the bee venom products produced by the current pharmaceutical factories is not ideal after use, and the bee venom products are far from being used for stinging by live bees. At present, no better collection mode of live bee venom and a method for detecting the weight of the bee venom exist at home and abroad, the application of the bee venom is severely restricted, and the development of industries such as live bee treatment, bee venom analysis research, production of related derivatives and the like is limited.
For live bees, different bee species, different breeding environments, different feeding methods, even by feeding sugar water containing medicinal components (such as Chinese herbal decoction), can affect the bee venom amount and bee venom activity of the live bees, so that there is a difference in bee venom weight of average individuals of the same age of day among live bees of different populations.
The existing production method of bee venom products, especially the method for producing liquid medicine or suppository by using powdery bee venom raw materials, loses part of effective substances in the powder preparation process, and the component proportion of the product is calculated by the powder, so that the bee venom product has poor effect. Even if the stability of products produced in different batches is influenced to a certain extent by the same formula, the effect of the final bee venom product can be influenced by different production processes or manufacturers and different quality control or factory detection standards. Some liquid bee venom products prepared by the existing manufacturers, even bee venom with the same volume, have great difference in the content and activity of bee venom. The volume of bee venom product produced by manufacturers can be known when the bee venom product is extracted by using a syringe, but the moisture in the bee venom is extremely volatile, so that the weight of the bee venom product is difficult to be objectively measured.
The method is characterized by completely collecting the live bee venom and screening the bees with stable quality through weight detection, and is favorable for improving the effect and the stability of related products in the bee venom product industry. However, there is no effective solution in the prior art.
Disclosure of Invention
1. Technical problem to be solved by the invention
In order to solve the problems in the prior art, the invention provides the bee venom collection device which is comprehensive in bee venom substance preservation, low in cost and simple in operation.
2. Technical scheme
In order to solve the problems, the technical scheme provided by the invention is as follows:
a bee venom collection device is characterized in that the pH value of a gel plate is 4.5-7.0, a hydrophobic elastic membrane is arranged on the surface of the gel, the hydrophobic elastic membrane is wetted by double-distilled water or deionized water, and solid gel in the gel plate is selected from gel which is converted into liquid when heated to 42-60 ℃.
Optionally, the gel plate is one of an agar plate, a medical gelatin plate, or a solid collagen egg white plate.
Optionally, the gel plate comprises chloramphenicol.
Optionally, the diameter of the gel plate is 2-8 cm.
Optionally, the hydrophobic elastic membrane is a rubber membrane or a latex membrane.
Optionally, the hydrophobic elastic membrane is a single layer or a plurality of layers
Optionally, the gel plate is an agar plate with agar in the agar plate.
Optionally, the agar has a thickness of 2-6 mm
3. Advantageous effects
Compared with the prior art, the technical scheme provided by the invention has the following beneficial effects:
(1) the bee venom collection device can completely collect live bee venom by using the gel plate, so that the situation that bee venom components are volatilized or degraded is avoided; the device can be used for detecting the weight and activity of bee venom products, and when the activity of the bee venom products is semi-quantitatively detected, the gel plate is an agar plate with red blood cells. The design of the hydrophobic elastic membrane can ensure that the humidity and the pH value of the gel plate are relatively constant, and bee needles can be left on the gel plate.
Drawings
FIG. 1 is a schematic view of the microstructure of a bee needle;
FIG. 2 is a block diagram illustrating a flow chart of a method for collecting bee venom of live bees according to an embodiment;
FIG. 3 is a block diagram illustrating a flowchart of a method for detecting bee venom weight of a live bee according to an embodiment;
fig. 4 is a schematic structural diagram of the bee venom collection and weight detection device in the embodiment.
In the figures, the reference numbers are: 1-bee and bee needle, 2-barb structure, 3-agar plate, 31-agar, 4-rubber film and 7-sealing cover.
Detailed Description
The following examples are given to illustrate specific embodiments of the present invention, but the present invention is not limited to the following examples.
The bee venom collection device shown in FIG. 4 comprises an agar plate 3, agar 31 disposed therein, a rubber membrane 4 disposed on the surface of the agar 31, and a sealing cap 7 for sealing the agar plate 3. The rubber film 4 is soaked in deionized water or double distilled water and then is tightly attached to the surface of the agar 31. The agar plate 3 is prepared by adopting agar, and chloramphenicol is added in the preparation process.
Wherein the diameter of the agar plate 3 is 2-8 cm, and the thickness of the agar is 2-6 mm.
A live bee venom collection method comprises the following steps: s1, preparing a gel plate with the pH value of 4.5-7.0, wherein a hydrophobic elastic film is arranged on the surface of gel in the gel plate; s2 pricking the gel plate with alive bee sting, and keeping the bee needle in the gel via hydrophobic elastic membrane, and continuously injecting bee venom; s3 removing the hydrophobic elastic membrane and the bee needle, and sealing the gel plate; in step S1, the gel plate may be an agar plate, a medical gelatin plate, or a solid collagen egg white plate. The collected bee venom can be directly stored for producing related products, and can be used without peeling the bee venom out. For example, the solid collagen plate can be directly used in the formula of the bee venom facial mask, or the solid collagen plate is made of human absorbable collagen, such as solid collagen made of catgut material. In addition, the gel can be used together with venenum Apis for grinding and making into suppository; in the step S1, selecting solid gel in the gel plate, wherein the solid gel is changed into liquid gel when being heated to 42-60 ℃; the live bee venom collection method further comprises the step S4 of heating the gel in the gel plate to 42-60 ℃, and sealing and storing the gel after the gel is converted from a solid state to a liquid state. The liquid bee venom containing gel can be directly used as raw material or processed into suppository; in the step S1, the hydrophobic elastic membrane is wetted by double distilled water or deionized water and can be tightly attached to the surface of the gel plate; optionally, in step S2, a plurality of bees can be respectively stung and stinging in the same gel plate at a time to obtain a larger amount of bee venom; in the step S2, the bee venom is continuously injected into the bee needles for 15-35 min. The bee venom glands at the tail ends of the bee needles after bee sting continuously move for a certain time, and bee venom in the bee venom glands is continuously injected into the gel plate, so that the bee needles are pulled out after staying for 15-35 min in the gel plate, and the obtained bee venom is more and more complete; in the step S2, the bee venom is continuously injected into the bee needles for 20-30 min. Bee venom is basically injected after 20min, the injected bee venom amount is less after 30min, the influence on the whole weight of bee venom is less, the activity of bee needles is basically lost, and bee venom in bee sacs cannot be injected continuously.
Compared with the prior art, the live bee venom collection method provided by the invention has the advantages that the gel is used for sealing and storing the bee venom after the live bee sting is stinged, on one hand, the gel can be used for bee venom weight detection, on the other hand, the gel and the live bee venom can be directly used as corresponding bee venom product processing raw materials together, and in addition, the weight of the bee venom in the gel is detected and obtained through a weight detection method during use. By arranging the hydrophobic elastic membrane on the surface of the gel, the bee needles of the live bees can be actively left in the gel plate and continuously injected into bee venom, so that the operation is convenient, and the collection of the bee venom is more complete. The pH value of the gel plate is in the same range as that of bee venom, so as to reduce the degradation rate of the internal substances of bee venom and prolong the storage time of the active substances in bee venom
A method for detecting the weight of live bee venom comprises the following steps:
the method comprises the following steps: s1, preparing a gel plate with the pH value of 4.5-7.0, weighing the gel plate to be m0, and arranging a hydrophobic elastic film on the surface of gel in the gel plate; s2 pricking the gel plate with alive bee sting, and keeping the bee needle in the gel via hydrophobic elastic membrane, and continuously injecting bee venom; s3, after bee venom injection is completed, taking down the hydrophobic elastic membrane and bee needles on the hydrophobic elastic membrane, weighing the weight of the gel plate at the moment to be m1, and sealing and storing the gel plate; s4, calculating the bee venom weight m2 to be m1-m 0; in step S20, a plurality of bees can sting each other in the same gel plate at a time to obtain a larger amount of bee venom. The accumulation of a plurality of bee venom increases the specific gravity of the bee venom relative to the gel plate, reduces the requirement of a balance, and enlarges the application range; in step S2, the selected bee populations, working ages, feeding conditions and/or feeding drugs that are sting in the same gel sheet are the same or similar. The total weight of the bee venom of the bees under the limited condition is convenient to count, and the average weight of the bee venom of each bee is calculated according to the number of the bees; in step S1, the gel plate may be an agar plate, a medical gelatin plate, or a solid collagen egg white plate. The collected bee venom can be directly stored for producing related products after weighing, and can be used without peeling the bee venom out. For example, the solid collagen egg white plate can be directly used in the formula of the bee venom facial mask; in the step S1, selecting solid gel in the gel plate, wherein the solid gel is changed into liquid gel when being heated to 42-60 ℃; in the step S3, the hermetically stored gel sheet is hermetically stored when heated to be liquid gel; in the step S1, the hydrophobic elastic membrane is wetted by double distilled water or deionized water and can be tightly attached to the surface of the gel plate; in step S2, a plurality of bees can be respectively sting in the same gel plate at one time to obtain a larger amount of bee venom; in the step S2, the bee venom is continuously injected into the bee needles for 15-35 min; in the step S2, the bee venom is continuously injected into the bee needles for 20-30 min; further comprising a step S5, when the bees are respectively stunted by the bees in the same gel plate in the step S2 to obtain more bee venom, the total amount of the bee venom obtained in the step S4 is obtained, and the average weight of the bee venom per bee is m2/n when the number of the bees is n.
Compared with the prior art, the live bee venom weight detection method provided by the invention has the advantages that the bee venom after the live bee sting is sealed and stored by the gel, the bee venom can be stored more completely and can be stored in the gel plate by the bee venom, and the bee venom weight data is obtained by weighing the mass change before and after the gel plate. In addition, the bee venom stinged by the live bees is sealed by the gel and can be directly used as a production raw material of a bee venom product after weighing is finished, in step S2, when the bee venom of the live bees is selected to be put into the agar 31 of the agar plate 3, the tail of the live bees is controlled to sting the agar 31 through the rubber film 4, because the inverted hook structure 2 arranged on the bee needle 1 is shown in figure 1, the bee needle 1 can be clamped by the rubber film 4, so that the bee needle 1 is separated from the bee body, at the moment, the bee needle 1 can continuously move for 15-30 min to continuously inject the bee venom in the venom sac into the agar 31, the step S3 is continued, after the injection of the bee venom is finished, the rubber film 4 and the bee needle 1 are taken down together, and the agar plate 3 is sealed and stored. The collected bee venom can be directly stored for producing related products, and can be used without peeling the bee venom out.
Since the collecting method and the weight detecting method are similar in part of the steps, they are described in the same embodiment.
It should be noted that the embodiment of the present invention is only one embodiment of the core idea of the present invention, and all the substantially same schemes such as equivalent replacement or simple improvement made on the basis of the method of the present invention belong to the protection scope of the present invention.

Claims (8)

1. The utility model provides a bee venom collection system which characterized in that: the gel plate comprises a gel plate, the pH value of the gel plate is 4.5-7.0, a hydrophobic elastic membrane is arranged on the surface of the gel, the hydrophobic elastic membrane is wetted by double-distilled water or deionized water, and solid gel in the gel plate is selected from gel which is converted into liquid when heated to 42-60 ℃.
2. The bee venom collection device according to claim 1, wherein: the gel plate is one of an agar plate, a medical gelatin plate or a solid collagen egg white plate.
3. The bee venom collection device according to claim 1, wherein: the gel plate contains chloramphenicol.
4. The bee venom collection device according to claim 1, wherein: the diameter of the gel plate is 2-8 cm.
5. The bee venom collection device according to claim 1, wherein: the hydrophobic elastic membrane is a rubber membrane or a latex membrane.
6. The bee venom collection device according to claim 1, wherein: the hydrophobic elastic membrane is a single layer or multiple layers.
7. A bee venom collection device according to claim 2, wherein: the gel plate is an agar plate, and agar is arranged in the agar plate.
8. The bee venom collection device according to claim 7, wherein: the thickness of the agar is 2-6 mm.
CN202111202896.8A 2019-12-26 2019-12-26 Bee venom collection system Pending CN113826566A (en)

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CN201911362611.XA CN110915726B (en) 2019-12-26 2019-12-26 Method and device for collecting live bee venom and detecting weight of bee venom
CN202111202896.8A CN113826566A (en) 2019-12-26 2019-12-26 Bee venom collection system

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US4739531A (en) * 1986-08-04 1988-04-26 Robson Charles H Bee venom collection apparatus
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CN204670114U (en) * 2015-05-20 2015-09-30 福建省神蜂科技开发有限公司 A kind of extractor of bee venon structure
CN106212395A (en) * 2016-08-30 2016-12-14 惠州学院 Red fire ant takes poison device
CN205813219U (en) * 2016-05-10 2016-12-21 云南胡飞飞农业科技有限公司 Bee venom collecting device

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FR453407A (en) * 1912-01-22 1913-06-07 Siemens Schuckertwerke Gmbh Tractor device for boats
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Publication number Priority date Publication date Assignee Title
US4739531A (en) * 1986-08-04 1988-04-26 Robson Charles H Bee venom collection apparatus
CN204047595U (en) * 2014-09-04 2014-12-31 杨仁勇 A kind of honeybee venon extractor
CN204670114U (en) * 2015-05-20 2015-09-30 福建省神蜂科技开发有限公司 A kind of extractor of bee venon structure
CN205813219U (en) * 2016-05-10 2016-12-21 云南胡飞飞农业科技有限公司 Bee venom collecting device
CN106212395A (en) * 2016-08-30 2016-12-14 惠州学院 Red fire ant takes poison device

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D.J.PALMER: "Extraction of Bee Venom for Research", BEE WORLD, vol. 42, no. 09, pages 225 *
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Application publication date: 20211224