CN110915598A - Leaf surface spraying liquid, method and illumination device for photoautotrophic rooting of sugarcane test-tube plantlets - Google Patents
Leaf surface spraying liquid, method and illumination device for photoautotrophic rooting of sugarcane test-tube plantlets Download PDFInfo
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Abstract
The invention belongs to the technical field of sugarcane planting, and particularly discloses a foliar spray liquid, a method and an illumination device for photoautotrophic rooting of sugarcane test-tube plantlets. The foliar spray liquid comprises indoleacetic acid, naphthylacetic acid, monopotassium phosphate, sucrose, L-proline and tween, and the combination of the raw materials effectively promotes the rooting effect of the sugarcane test-tube plantlet and improves the survival rate of the test-tube plantlet; the invention also provides a photoautotrophic rooting method for sugarcane test-tube plantlets and an illumination device, wherein the illumination device comprises electric telescopic vertical rods and transverse rods which are mutually connected, a connecting rod is arranged between the transverse rods, a lamp holder group is connected onto the connecting rod, four lamp holders are arranged on the lamp holder group, and a fluorescent lamp, a red light lamp, a blue light lamp and a near ultraviolet lamp are respectively arranged on the four lamp holders; a control system is also included. According to the method, the specially-made leaf surface spraying liquid and the illumination device are combined to process the sugarcane test-tube plantlets, so that the survival rate and the quality of the sugarcane test-tube plantlets are effectively improved.
Description
[ technical field ] A method for producing a semiconductor device
The invention belongs to the technical field of sugarcane planting, and particularly relates to a leaf surface spraying liquid, a method and an illumination device for photoautotrophic rooting of sugarcane test-tube plantlets.
[ background of the invention ]
At present, the method of stem tip tissue culture is generally adopted at home and abroad to obtain detoxified healthy seedlings, and the production scale of healthy sugarcane seedlings in Guangxi areas is increased year by year. As an effective detoxification technology, the tissue culture technology of the sugarcane can weaken the diseases such as mosaic disease, perennial root dwarfing disease and the like on sugarcane plants which are difficult to prevent and control by using a conventional method, and the produced healthy seedlings are used for production, so that the yield increasing and sugar increasing effects are obvious. Because the production process of the healthy sugarcane seedlings is complex and time-consuming, it is important to simplify the production and obtain a large number of healthy sugarcane seedlings through rapid breeding. However, most sugarcane test-tube plantlets are rooted in the bottle and then transplanted to the external environment. Because the growth cycle of the sugarcane test-tube plantlet in the bottle is long, and the transplanting survival rate is low, the production cost is high, and the popularization and the planting of the sugarcane test-tube plantlet are hindered.
The ex vitro rooting technology of the test-tube plantlet, namely the photoautotrophic rooting of sugarcane is an advanced tissue culture rooting technology which is successfully researched in recent years and is an important component of simplified production technology of the plant test-tube plantlet. The sugarcane photoautotrophic rooting method is characterized in that after the rootless test-tube plantlets are sprayed with the liquid surface rooting solution, the rootless test-tube plantlets are directly cultivated in a greenhouse nursery garden for rooting, the traditional rooting stage and the domestication stage are combined, the traditional procedure that the rootless test-tube plantlets grow roots in test tubes is omitted, the autotrophic rooting replaces the traditional heterotrophic rooting, the seedling culture period is shortened, and the production cost is saved. However, the photoautotrophic roots are greatly influenced by environmental conditions, so the photoautotrophic roots are basically carried out in spring and summer with proper temperature, humidity and illumination (namely the average daily temperature exceeds 23 ℃), and in winter, the transplanting survival rate and rooting rate of the sugarcane test-tube plantlets are generally low in the environment with the average daily temperature lower than 15 ℃ and low humidity and illumination.
There are also related studies to address the above problems. For example, Chinese patent application CN 109042666A discloses a foliar spray liquid formula for improving the survival rate of sugarcane test-tube plantlets and a use method for applying the foliar spray liquid formula to the rooting of sugarcane test-tube plantlets, so that the transplanting survival rate of the sugarcane rootless test-tube plantlets is as high as 82.3 percent; similarly, the chinese patent application CN 104920211 a discloses a growth promoting solution and a method for improving the photoautotrophic rooting efficiency of sugarcane test-tube plantlets, which also optimizes the formula of the foliar spray solution and applies it to the sugarcane test-tube plantlets, so that the highest survival rate of the transplanted sugarcane test-tube plantlets is 92%, although the survival rate of the test-tube plantlets is improved to a certain extent by the above two methods, the effect is still not ideal; the Chinese patent application CN 104273030A discloses a method for transplanting sugarcane test-tube plantlets after soaking by sequentially adopting plant growth regulator and auxin mixed liquor and then carrying out humidity management, and although the transplanting survival rate of the sugarcane test-tube plantlets is up to 95 percent by the method, the rooting efficiency of the test-tube plantlets is still low.
[ summary of the invention ]
In view of the above, in order to improve the transplanting effect of the sugarcane test-tube plantlet in winter, improve the annual use efficiency of the greenhouse and provide a large number of cluster-planted plantlets for the separate plant transplanting in spring of the next year, it is necessary to provide a foliar spray solution, a method and an illumination device for the photoautotrophic rooting of the sugarcane test-tube plantlet, wherein the foliar spray solution can effectively improve the rooting efficiency and the survival rate of the sugarcane test-tube plantlet, and meanwhile, the survival rate and the quality of the sugarcane test-tube plantlet are further improved by combining a specific illumination device and a special foliar spray solution for the photoautotrophic rooting management and cultivation of the sugarcane test-tube plantlet.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a sugarcane test-tube plantlet photoautotrophic rooting foliar spray liquid comprises the following raw material components: 45-55mg/L of indoleacetic acid, 15-25mg/L of naphthylacetic acid, 2-4g/L of monopotassium phosphate, 15-25 g/L, L g of sucrose, 90-110mg/L of proline and 1-3ml/L of Tween.
Further, the sugarcane test-tube plantlet photoautotrophic rooting foliar spray liquid comprises the following raw material components in parts by weight: 50mg/L of indoleacetic acid, 20mg/L of naphthylacetic acid, 3g/L of monopotassium phosphate, 20g/L, L g/100 mg/L of sucrose and 2ml/L of Tween.
The invention also provides a photoautotrophic rooting method for sugarcane test-tube plantlets, which is to spray the sugarcane test-tube plantlets by using the foliar spray liquid as claimed in claim 1 or 2.
Further, the photoautotrophic rooting method for the sugarcane test-tube plantlets comprises the following steps:
(1) hardening seedlings: moving the rootless subculture sugarcane test-tube plantlets to a shade place, then opening a test-tube cover, spraying the leaf surface spraying liquid on the test-tube plantlets, and then hardening the plantlets in a greenhouse for 24 hours;
(2) grouping and sterilizing: dividing the test-tube plantlets after hardening into 4-10 plants/cluster, then putting the test-tube plantlet cluster into clear water for cleaning for 2-3 times, and draining; soaking the drained test-tube plantlet in disinfectant for 10-20 min;
(3) planting: planting the sterilized test-tube plantlets in a planting tray with a planting matrix, and pouring enough root fixing water;
(4) and (3) rooting management: moving the planting tray into a seedling raising greenhouse provided with an illumination device, and placing the planting tray according to three trays/rows; after placing the completion, surround at the outer plastic film of illumination device, adopt again illumination device shines the processing to test tube seedling, specifically is:
1-5d after planting, firstly adopting white light with the illumination intensity of 5000-;
6-10d after the planting, firstly adopting white light with the illumination intensity of 6000-;
after the 10 th day, white light with the illumination intensity of 5000-.
Further, in the step (2), the disinfectant is a thiophanate solution with a concentration of 15%.
Further, in the step (3), the planting substrate is prepared by mixing yellow mud, peat soil and calcium magnesium phosphate fertilizer according to a weight ratio of 50 kg: 500 g: 100g of the raw materials are mixed; the planting tray is 6-9 holes in specification.
The invention also provides the illumination device, which comprises four electric telescopic vertical rods and two cross rods, wherein two ends of one cross rod are respectively connected with the top ends of the two electric telescopic vertical rods, and two ends of the other cross rod are respectively connected with the top ends of the other two electric telescopic vertical rods; a plurality of connecting rods are arranged between the two cross rods at equal intervals, two ends of each connecting rod are respectively connected with the rod bodies of the two cross rods, a plurality of lamp holder groups are connected to the connecting rods at equal intervals, four lamp holders are arranged on the lamp holder groups at equal intervals, the four lamp holders are arranged along the length direction of the connecting rods, and a fluorescent lamp, a red light lamp, a blue light lamp and a near ultraviolet lamp are respectively arranged on the four lamp holders; still include control system, control system includes control operation panel, warning light and distance sensor, be equipped with controller and time-recorder in the control operation panel, the control operation panel upper surface is control panel, the warning light install in control panel is last, distance sensor install in the top of electronic flexible montant or on the connecting rod, the controller respectively with control panel the warning light the time-recorder distance sensor, four electronic flexible montant and four the lamp stand is connected.
By adopting the technical scheme of the invention, the invention has the following beneficial effects:
firstly, the foliar spray liquid of the invention effectively enhances the stress resistance of the test-tube plantlet and promotes the growth of the test-tube plantlet by compounding the potassium dihydrogen phosphate and the combination of the heteroauxin, the naphthylacetic acid, the sucrose, the L-proline and the Tween, thereby effectively improving the survival rate and the rooting effect of the test-tube plantlet. The content of the monopotassium phosphate is controlled to be 2-4g/L, the survival rate of the sugarcane test-tube plantlet can be effectively improved, the quality of the test-tube plantlet can be improved to a certain extent, and experimental tests show that when the content of the monopotassium phosphate in the foliar spray liquid is lower than 2g/L, the stem thickness of the test-tube plantlet planted for 45d is remarkably reduced to 6.83mm, and when the content of the monopotassium phosphate is higher than 4g/L, the number of the test-tube plantlets planted for 45d is only 9.3, and the number of the test-tube plantlets is remarkably reduced.
In addition, the illumination device irradiates the sugarcane test-tube plantlet by adopting different light combinations in stages, so that the temperature, humidity and illumination intensity in the enclosure can be improved, the leaf extension and root development of the sugarcane test-tube plantlet are effectively promoted, and the survival rate and quality of the test-tube plantlet are effectively improved.
Thirdly, the invention adjusts the irradiation of the sugarcane test-tube plantlet through the matched illumination system, has high automation degree and convenient use, thereby improving the survival rate of the test-tube plantlet and promoting the growth of the test-tube plantlet, and has obvious effect.
[ description of the drawings ]
Fig. 1 is a schematic structural view of an illumination device of the present invention.
Fig. 2 is a schematic structural diagram of the lamp socket set in fig. 1.
Fig. 3 is a schematic diagram of the connection structure of the relevant components of the control system of the present invention.
Description of the main elements
In the figure, 1-electric telescopic vertical rod, 11-base, 2-cross rod, 3-connecting rod, 4-lamp holder group, 41-fluorescent lamp, 42-red lamp, 43-blue lamp, 44-near ultraviolet lamp, 5-distance sensor, 6-control console, 61-control panel, 611-display screen, 612-touch key area and 7-warning lamp.
The following detailed description will further illustrate the invention in conjunction with the above-described figures.
[ detailed description ] embodiments
The invention will now be further described with reference to specific examples.
The specific structure of the illumination device used in the following examples is as follows:
as shown in fig. 1-3, in a preferred embodiment of the present invention, the illumination device includes four electric telescopic vertical bars 1 and two cross bars 2, wherein two ends of one cross bar 2 are respectively vertically connected to top ends of two electric telescopic vertical bars 1, two ends of the other cross bar 2 are respectively vertically connected to top ends of the other two electric telescopic vertical bars 1, and bottom ends of the four electric telescopic vertical bars 1 are respectively connected to a base 11; two the equidistance is equipped with a plurality of connecting rods 3 between the horizontal pole 2, the both ends of connecting rod 3 respectively with two the pole body of horizontal pole 2 is connected perpendicularly, the equidistance is connected with a plurality of lamp stand group 4 on the bottom surface of connecting rod 3, equidistant four lamp stands, four being equipped with on the lamp stand group 4 the lamp stand is followed the length direction of connecting rod 3 sets up, four install fluorescent lamp 41, red light lamp 42, blue light lamp 43 and near ultraviolet lamp 44 on the lamp stand respectively, establish ties between the lamp stand of the same colour lamp of installation.
The illumination device further comprises a control system, the control system comprises a control operation platform 6, a warning lamp 7 and a distance sensor 5, a controller and a timer are arranged in the control operation platform 6, the upper surface of the control operation platform 6 is provided with a control panel 61, the controller is respectively electrically connected with the control panel 61, the warning lamp 7, the timer, the distance sensor 5, the four electric telescopic vertical rods 1 and the four lamp holders, and the control panel 61 comprises a display screen 611 and a touch setting key area 612 and is used for selecting corresponding functions to operate according to needs; the warning lamp 7 is mounted on the control panel 61 and used for displaying different colors according to different light irradiation, so that an operator can directly know the light irradiation state conveniently, in the invention, when the fluorescent lamp 41 is used for irradiation, the warning lamp 7 displays yellow, when the red light lamp 42 is used for irradiation, the warning lamp 7 displays red, when the blue light lamp 43 is used for irradiation, the warning lamp 7 displays blue, and when the near ultraviolet lamp 44 is used for irradiation, the warning lamp 7 displays purple; the distance sensor 5 is arranged on the connecting rod 3 and used for detecting the distance between the connecting rod 3 and the bottom end of the electric telescopic vertical rod 1 and transmitting a distance signal to the controller; the timer is used for timing the illumination time of each lamp.
The use method of the illumination device comprises the following steps:
(1) stably placing the bases 11 of the four electric telescopic rods 1 on the ground of a seedling raising greenhouse, enabling each connecting rod 3 to be correspondingly positioned right above each seed sowing tray, and then surrounding the top, the periphery and the top of the illumination device by using a plastic film to finish installation;
(2) pressing a power-on button on the touch button area 612, wherein the illumination device is in a power-on state at this time;
(3) selecting an illumination program to be operated, setting corresponding parameters, and starting to operate. For example:
white light-red light-near ultraviolet light irradiation procedure: pressing a 'first-stage irradiation program' key on the touch key area 612, then setting the time of the whole irradiation process through a total operation time button, setting the irradiation time of each light through an illumination time setting button, setting the irradiation intensity of each light through an illumination intensity setting button, and setting the height of the connecting rod through a height setting button, and then pressing a start button on the touch key to start entering a white light-red light-near ultraviolet irradiation program, namely, the controller adjusts the state of the corresponding appliance according to the set parameters to enable the operation state of the corresponding appliance to be consistent with the set parameters, in the operation state, when the fluorescent lamp starts to irradiate according to the set illumination intensity, the controller sends a timing start instruction to the timer, the timer starts to time, when the counted time of the timer reaches a set value, the controller sends a fluorescent lamp power-off instruction, a red light lamp connection instruction and a re-timing instruction according to a received timing signal fed back by the timer, at the moment, the fluorescent lamp stops irradiation when the power is off, the red light lamp starts irradiation, and the timer restarts timing, when the time counted by the timer reaches a set value, the controller sends a red light lamp power-off instruction, a near ultraviolet lamp connection instruction and a re-timing instruction according to the received timing signal fed back by the timer, at the moment, the red light lamp stops irradiation when the power is off, the near ultraviolet lamp starts irradiation, and the timer restarts timing, and when the time counted by the timer reaches the set value, the controller starts to cycle again until the total operation time is finished.
When other illumination programs need to be operated, the corresponding buttons are pressed and relevant parameters are set.
The types, structures and action principles of the controller, the timer, the distance sensor, the electric telescopic vertical rod, the fluorescent lamp, the red light lamp, the near ultraviolet lamp and the blue light lamp, an electric connection method among the controller and the control method of the controller belong to the prior art, and are not repeated herein for saving space.
In addition, in the invention, the variety of the sugarcane test-tube plantlet is New Tabane No. 22, which is provided by the research institute of sugarcane of Guangxi academy of agricultural sciences; indolylacetic acid, naphthylacetic acid and potassium dihydrogen phosphate are purchased from national medicine group chemical reagent limited, L-proline is purchased from Kyowa Kong chemical company, Tween is purchased from Guangxi Deng Fine chemical research institute in Tianjin, sucrose is purchased from Nanning sugar industry Co., Ltd, peat soil is purchased from Changchun Seisaku agricultural development Limited liability company, and calcium phosphate fertilizer is purchased from Yunnan Kunming phosphate fertilizer company.
Example 1
The leaf surface spraying liquid for the sugarcane test-tube plantlets comprises the following raw material components in parts by weight: 45mg/L of indoleacetic acid, 15mg/L of naphthylacetic acid, 2g/L of monopotassium phosphate, 15 g/L, L g/L of sucrose and 90mg/L of proline and 1ml/L of Tween.
A photoautotrophic rooting method for sugarcane test-tube plantlets by using the liquid level spraying liquid comprises the following steps:
(1) hardening seedlings: moving the rootless subculture sugarcane test-tube plantlets to a shade place, then opening a test-tube cover, spraying the leaf surface spraying liquid on the test-tube plantlets, and then hardening the plantlets for 24 hours in the natural environment of a greenhouse;
(2) grouping and sterilizing: dividing the test-tube plantlets after hardening into 4 plants/clumps, then putting the test-tube plantlet clumps into clear water for cleaning for 2 times, and draining; soaking the drained test-tube plantlet in a thiophanate solution with the concentration of 15% for 10 min;
(3) planting: planting the disinfected test-tube plantlets in a container containing 50kg of yellow mud, peat soil and calcium magnesium phosphate fertilizer: 500 g: mixing 100g of the planting substrate in a planting tray (6 x 9 holes) and pouring enough root fixing water;
(4) and (3) rooting management: moving the planting tray into a seedling raising greenhouse provided with an illumination device, and placing the planting tray according to three trays/rows; after placing the completion, adopt illumination device shines the processing to the test tube seedling, specifically is:
irradiating the planted 1 st to 5 th days by using white light with the illumination intensity of 5000lx for 8h, then irradiating the planted plants by using red light with the illumination intensity of 3000lx for 3h, and then irradiating the planted plants by using near ultraviolet light with the illumination intensity of 2000lx for 1h, and circulating the irradiation operation;
irradiating the planted plants at 6-10d for 5h by using white light with the illumination intensity of 6000lx, irradiating the plants for 2h by using red light with the illumination intensity of 2000lx, irradiating the plants for 4h by using white light with the illumination intensity of 6000lx, and irradiating the plants for 1h by using blue light with the illumination intensity of 1000lx, and circulating the irradiation operation;
after the 10 th day of planting, white light with the illumination intensity of 5000lx is adopted for illumination.
Example 2
The leaf surface spraying liquid for the sugarcane test-tube plantlets comprises the following raw material components in parts by weight: 50mg/L of indoleacetic acid, 20mg/L of naphthylacetic acid, 3g/L of monopotassium phosphate, 20g/L, L g/100 mg/L of sucrose and 2ml/L of Tween.
A photoautotrophic rooting method for sugarcane test-tube plantlets by using the liquid level spraying liquid comprises the following steps:
(1) hardening seedlings: moving the rootless subculture sugarcane test-tube plantlets to a shade place, then opening a test-tube cover, spraying the leaf surface spraying liquid on the test-tube plantlets, and then hardening the plantlets for 24 hours in the natural environment of a greenhouse;
(2) grouping and sterilizing: dividing the test-tube plantlets after hardening into 8 plants/cluster, then putting the test-tube plantlet cluster into clear water for cleaning for 3 times, and draining; soaking the drained test-tube plantlet cluster in a thiophanate solution with the concentration of 15% for 15 min;
(3) planting: planting the disinfected test-tube plantlets in a container containing 50kg of yellow mud, peat soil and calcium magnesium phosphate fertilizer: 500 g: mixing 100g of the planting substrate in a planting tray (6 x 9 holes) and pouring enough root fixing water;
(4) and (3) rooting management: moving the planting tray into a seedling raising greenhouse provided with an illumination device, and placing the planting tray according to three trays/rows; after placing the completion, adopt illumination device shines the processing to the test tube seedling, specifically is:
irradiating the planted 1 st to 5 th days by using white light with the illumination intensity of 6500lx for 8h, then irradiating the planted plants by using red light with the illumination intensity of 3500lx for 3h, and then irradiating the planted plants by using near ultraviolet light with the illumination intensity of 2500lx for 1h, and circulating the irradiation operation;
irradiating the planted plants at 6-10d by adopting white light with the illumination intensity of 7000lx for 5h, then irradiating the plants by adopting red light with the illumination intensity of 2500lx for 2h, then irradiating the plants by adopting white light with the illumination intensity of 7000lx for 4h, finally irradiating the plants by adopting blue light with the illumination intensity of 1500lx for 1h, and circulating the irradiation operation;
after the 10 th planting, white light with the illumination intensity of 7000lx is adopted for illumination.
Example 3
The leaf surface spraying liquid for the sugarcane test-tube plantlets comprises the following raw material components in parts by weight: 55mg/L of indoleacetic acid, 25mg/L of naphthylacetic acid, 4g/L of monopotassium phosphate, 25 g/L, L g/L of sucrose and 110mg/L of proline and 3ml/L of Tween.
A photoautotrophic rooting method for sugarcane test-tube plantlets by using the liquid level spraying liquid comprises the following steps:
(1) hardening seedlings: moving the rootless subculture sugarcane test-tube plantlets to a shade place, then opening a test-tube cover, spraying the leaf surface spraying liquid on the test-tube plantlets, and then hardening the plantlets for 24 hours in the natural environment of a greenhouse;
(2) grouping and sterilizing: dividing the test-tube plantlets after hardening into 10 plants/cluster, then putting the test-tube plantlet cluster into clear water for cleaning for 3 times, and draining; soaking the drained test-tube plantlet in a thiophanate solution with the concentration of 15% for 20 min;
(3) planting: planting the disinfected test-tube plantlets in a container containing 50kg of yellow mud, peat soil and calcium magnesium phosphate fertilizer: 500 g: mixing 100g of the planting substrate in a planting tray (6 x 9 holes) and pouring enough root fixing water;
(4) and (3) rooting management: moving the planting tray into a seedling raising greenhouse provided with an illumination device, and placing the planting tray according to three trays/rows; after placing the completion, adopt illumination device shines the processing to the test tube seedling, specifically is:
irradiating the planted 1 st to 5 th days by adopting white light with the illumination intensity of 8000lx for 8h, then irradiating by adopting red light with the illumination intensity of 4000lx for 3h, and then irradiating by adopting near ultraviolet light with the illumination intensity of 3000lx for 1h, and circulating the irradiation operation;
irradiating the planted plants in 6 th to 10 th days by adopting white light with the illumination intensity of 8000lx for 5h, then irradiating the plants by adopting red light with the illumination intensity of 3000lx for 2h, then irradiating the plants by adopting white light with the illumination intensity of 8000lx for 4h, and finally irradiating the plants by adopting blue light with the illumination intensity of 1000-;
after the 10 th day of planting, white light with an illumination intensity of 8000lx is used for illumination.
Effect verification
1. Influence of foliar spray liquid on sugarcane test-tube plantlet
control group 1: the test-tube plantlet obtained by the photoautotrophic rooting method of the sugarcane test-tube plantlet which is sprayed by adopting the foliage spray liquid basically the same as that of the embodiment 2 of the invention is different in that: no monopotassium phosphate was included, i.e. the group of foliar sprays included: 50mg/L of indoleacetic acid, 20mg/L of naphthylacetic acid, 20g/L, L g/100 mg/L of sucrose-proline and 2ml/L of Tween;
control group 2: the test-tube plantlet obtained by the photoautotrophic rooting method of the sugarcane test-tube plantlet which is sprayed by the foliage spraying liquid (namely the spraying liquid recorded in the Chinese patent application CN 109042666A) basically same as the leaf surface spraying liquid of the control group 1 of the invention is different only in part of raw material contents: 20mg/L of indoleacetic acid, 10mg/L of naphthylacetic acid, 20g/L, L g/100 mg/L of sucrose-proline and 2ml/L of Tween;
control group 3: the test-tube plantlet obtained by the photoautotrophic rooting method of the sugarcane test-tube plantlet which is sprayed by the foliar spray liquid basically the same as that of the embodiment 2 of the invention is different only in that the contents of part of raw materials are different: 20mg/L of indolebutyric acid, 10mg/L of naphthylacetic acid, 3g/L of monopotassium phosphate, 20g/L of sucrose, 100mg/L of proline and 802 ml/L of tween-L;
control group 4: the test-tube plantlet obtained by the photoautotrophic rooting method of the sugarcane test-tube plantlet which is sprayed by adopting the foliage spray liquid basically the same as that of the embodiment 2 of the invention is different in that: potassium sulfate is used for replacing monopotassium phosphate, namely the group of foliar spray liquid comprises: 50mg/L of indoleacetic acid, 20mg/L of naphthylacetic acid, 3g/L of potassium sulfate, 20g/L, L g/100 mg/L of sucrose and 2ml/L of tween;
control group 5: the test-tube plantlet obtained by the photoautotrophic rooting method of the sugarcane test-tube plantlet which is sprayed by adopting the foliage spray liquid basically the same as that of the embodiment 2 of the invention is different in that: the calcium superphosphate is used for replacing potassium dihydrogen phosphate, namely the group of foliar spray liquid comprises: 50mg/L of indoleacetic acid, 20mg/L of naphthylacetic acid, 3g/L of calcium superphosphate, 20g/L, L g/100 mg/L of sucrose and 2ml/L of tween;
control group 6: the test-tube plantlet obtained by the photoautotrophic rooting method of the sugarcane test-tube plantlet which is sprayed by adopting the foliage spray liquid basically the same as that of the embodiment 2 of the invention is different in that: ammonium sulfate was used instead of monopotassium phosphate, i.e. the group of foliar spray solutions included: 50mg/L of indoleacetic acid, 20mg/L of naphthylacetic acid, 3g/L of ammonium sulfate, 20g/L, L g of sucrose, 100mg/L of proline and 2ml/L of tween;
control group 7: the test-tube plantlet obtained by the photoautotrophic rooting method of the sugarcane test-tube plantlet which is sprayed by adopting the foliage spray liquid basically the same as that of the embodiment 2 of the invention is different in that: gibberellin is used instead of naphthylacetic acid, i.e. the group of foliar spray solutions comprises: 50mg/L of indoleacetic acid, 20mg/L of gibberellin, 3g/L of ammonium sulfate, 20g/L, L g of sucrose, 100mg/L of proline and 2ml/L of Tween.
The photoautotrophic rooting methods for the sugarcane test-tube plantlets in the experimental groups 1-3 are respectively as in examples 1-3, while the photoautotrophic rooting methods for the sugarcane test-tube plantlets in the control groups 1-6 are all the same as in example 2 (i.e., the operation steps are the same except that the foliar spray fertilizers used in each group are different). 256 plants are planted in each group, and the survival rate of each group of test-tube plantlets is investigated after the test-tube plantlets are planted for 30 days (the statistical results are shown in table 1); after planting the test-tube plantlet 45d, randomly extracting 30 plants from each group of test-tube plantlets, cleaning each test-tube plantlet and sucking water, and investigating the root growth condition of each group of test-tube plantlets (the statistical result is shown in table 2), wherein:
(1) the survival rate calculation formula is as follows: survival (%) — number of surviving plants/number of investigated plants × 100%;
(2) root length determination: measuring the length of the longest root of each test-tube plantlet by using a ruler, adding the longest root lengths of 30 test-tube plantlets, and dividing by 30 to obtain the root length of the test-tube plantlet;
(3) and (3) root number determination: and (4) counting the large root number of each test-tube plantlet, adding the large root numbers of 30 test-tube plantlets, and dividing by 30 to obtain the root number of the test-tube plantlet.
TABLE 1 influence of foliar spray on the survival rate of sugarcane test-tube plantlets
| Group of | Number of plants/plant | Number of surviving plants/plant | Survival rate/%) |
| |
256 | 247 | 96.4 |
| |
256 | 251 | 98.0 |
| |
256 | 243 | 94.9 |
| |
256 | 210 | 82.0 |
| |
256 | 220 | 85.9 |
| |
256 | 213 | 83.2 |
| |
256 | 198 | 77.3 |
| |
256 | 206 | 80.5 |
| |
256 | 191 | 74.6 |
| |
256 | 179 | 69.9 |
As can be seen from Table 1, the survival rate of the sugarcane test-tube plantlets in the experimental groups 1-3 is as high as 97.3%, which is obviously superior to that of the control groups 1-7, and the foliar spray liquid provided by the invention can effectively promote the growth of the sugarcane test-tube plantlets and has high transplanting survival rate. As can be seen from comparison between the experimental group 2 and the control groups 1 to 3, in the absence of monopotassium phosphate, the survival rate of the sugarcane test-tube plantlet is higher under the contents of the indoleacetic acid and the naphthylacetic acid of the control group 2, but the survival rate of the sugarcane test-tube plantlet is reduced by adding the monopotassium phosphate under the contents of the indoleacetic acid and the naphthylacetic acid, so that the indoleacetic acid and the naphthylacetic acid (namely the control group 1) under the contents of the invention can interact with the monopotassium phosphate, and the survival rate of the sugarcane test-tube plantlet can be effectively improved; it can be known from comparison of experimental group 2, control group 1 and control group 4-7 that the survival rate of the sugarcane test-tube plantlet obtained by replacing potassium dihydrogen phosphate with a chemical fertilizer with the same property and replacing naphthylacetic acid with a growth promoter with the same property is not improved but is reduced compared with that of control group 1, so that the method can be used for demonstrating that the synergistic effect is achieved between the potassium dihydrogen phosphate (if only potassium dihydrogen phosphate solution is adopted for soaking and spraying, the survival rate of the sugarcane test-tube plantlet is lower than 37%) and the combination formed by indoleacetic acid, naphthylacetic acid, sucrose, L-proline and tween, the survival rate of the sugarcane test-tube plantlet can be effectively improved, and the components can not be replaced randomly and routinely.
TABLE 2 influence of foliar spray on root System of sugarcane test-tube plantlets
| Group of | Root length/cm | Number of root/root |
| |
25.33 | 11.8 |
| |
25.67 | 10.6 |
| |
25.19 | 11.3 |
| |
24.08 | 9.6 |
| |
24.18 | 9.8 |
| |
23.87 | 9.2 |
| |
21.59 | 8.2 |
| |
22.73 | 8.5 |
| |
21.49 | 8.0 |
| |
22.87 | 8.7 |
As can be seen from Table 2, the root length and the number of the test-tube plantlets of the sugarcane in the experimental groups 1 to 3 are obviously superior to those of the control groups 1 to 7, so that the potassium dihydrogen phosphate, the combination of the indoleacetic acid, the naphthylacetic acid, the sucrose, the L-proline and the Tween have a synergistic effect, the root growth of the test-tube plantlets of the sugarcane can be effectively promoted, and the quality of the test-tube plantlets of the sugarcane is improved.
2. Influence of illumination on sugarcane test-tube plantlet
Experimental groups: the method for the photoautotrophic rooting of the sugarcane test-tube plantlets in the embodiment 1 of the invention is adopted to cultivate the obtained test-tube plantlets;
control group 1: the test-tube plantlet obtained by the method basically the same as the photoautotrophic rooting method of the sugarcane test-tube plantlet in the embodiment 1 of the invention is cultivated, and the difference is that the irradiation treatment is as follows: after planting, firstly adopting white light with the illumination intensity of 5000lx to irradiate for 8h, then adopting red light with the illumination intensity of 3000lx to irradiate for 3h, then adopting near ultraviolet light with the illumination intensity of 2000lx to irradiate for 1h, and continuously circulating the irradiation operation;
control group 2: the test-tube plantlet obtained by the method basically the same as the photoautotrophic rooting method of the sugarcane test-tube plantlet in the embodiment 1 of the invention is cultivated, and the difference is that the irradiation treatment is as follows: after planting, firstly irradiating for 5 hours by adopting white light with the illumination intensity of 6000lx, then irradiating for 2 hours by adopting red light with the illumination intensity of 2000lx, then irradiating for 4 hours by adopting white light with the illumination intensity of 6000lx, and finally irradiating for 1 hour by adopting blue light with the illumination intensity of 1000lx, and continuously circulating the irradiation operation;
control group 3: the test-tube plantlet obtained by the method basically the same as the photoautotrophic rooting method of the sugarcane test-tube plantlet in the embodiment 1 of the invention is cultivated, and the difference is that the irradiation treatment is as follows: after planting, white light with the illumination intensity of 5000lx is adopted for illumination.
200 test-tube plantlets are planted in each group, and the survival rate of each test-tube plantlet group is investigated after the test-tube plantlets are planted for 30 days (the statistical result is shown in table 3); after 45d of test-tube plantlets were planted, 30 plantlets were randomly extracted from each group of test-tube plantlets, each test-tube plantlet was cleaned and water was sucked dry, and the growth of each test-tube plantlet was investigated (see table 4 for statistical results), wherein:
(1) the survival rate calculation formula is as follows: survival (%) — number of surviving plants/number of investigated plants × 100%;
(2) and (3) measuring the seedling height: measuring the length from the root of the stem to the tip of the longest blade of each test-tube seedling as the height of the seedling, adding the heights of 30 test-tube seedlings, and dividing the sum by 30 to obtain the height of the test-tube seedling;
(3) stem thickness measurement: measuring the thickest stem part of each test-tube plantlet, adding the thickest stem parts of 30 test-tube plantlets, and dividing the added thickest stem parts by 30 to obtain the stem thickness of the test-tube plantlet.
TABLE 3 Effect of light on survival of test-tube plantlets
| Group of | Number of plants/plant | Number of surviving plants/plant | Survival rate/%) |
| Experimental group | 200 | 191 | 95.5 |
| |
200 | 181 | 90.5 |
| |
200 | 173 | 86.5 |
| |
200 | 176 | 88.0 |
As can be seen from Table 3, the survival rate of the test-tube plantlets in the experimental group is obviously superior to that of the control groups 1-3, which shows that the survival rate of the test-tube plantlets can be effectively improved by irradiating the sugarcane test-tube plantlets with different lights in stages.
TABLE 4 Effect of light on growth of test-tube plantlets
| Group of | Height/cm of seedling | Thickness of stem/mm |
| Experimental group | 79.4 | 7.38 |
| |
67.8 | 7.03 |
| |
65.9 | 7.14 |
| |
71.5 | 6.97 |
As can be seen from Table 4, the seedling height and stem thickness of the test-tube plantlet of the experimental group are obviously superior to those of the control group 1-3, which shows that the method of the invention adopts different lights to irradiate the sugarcane test-tube plantlet in stages to effectively promote the growth of the test-tube plantlet and the test-tube plantlet has high quality.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (8)
1. The photosynthetic autotrophic rooting foliar spray liquid for the sugarcane test-tube plantlets is characterized by comprising the following raw material components in parts by weight: 45-55mg/L of indoleacetic acid, 15-25mg/L of naphthylacetic acid, 2-4g/L of monopotassium phosphate, 15-25 g/L, L g of sucrose, 90-110mg/L of proline and 1-3ml/L of Tween.
2. The photoautotrophic rooting foliar spray solution for sugarcane test-tube plantlets according to claim 1 is characterized by comprising the following raw material components in parts by weight: 50mg/L of indoleacetic acid, 20mg/L of naphthylacetic acid, 3g/L of monopotassium phosphate, 20g/L, L g/100 mg/L of sucrose and 2ml/L of Tween.
3. A photoautotrophic rooting method for sugarcane test-tube plantlets, characterized in that the sugarcane test-tube plantlets are sprayed with the foliar spray of claim 1 or 2.
4. The photoautotrophic rooting method for sugarcane test-tube plantlets according to claim 3 is characterized by comprising the following steps:
(1) hardening seedlings: moving the rootless subculture sugarcane test-tube plantlets to a shade place, then opening a test-tube cover, spraying the leaf surface spraying liquid on the test-tube plantlets, and then hardening the plantlets in a greenhouse for 24 hours;
(2) grouping and sterilizing: dividing the test-tube plantlets after hardening into 4-10 plants/cluster, then putting the test-tube plantlet cluster into clear water for cleaning for 2-3 times, and draining; soaking the drained test-tube plantlet in disinfectant for 10-20 min;
(3) planting: planting the sterilized test-tube plantlets in a planting tray with a planting matrix, and pouring enough root fixing water;
(4) and (3) rooting management: moving the planting tray into a seedling raising greenhouse provided with an illumination device, and placing the planting tray according to three trays/rows; after placing the completion, surround at the outer plastic film of illumination device, adopt again illumination device shines the processing to test tube seedling, specifically is:
1-5d after planting, firstly adopting white light with the illumination intensity of 5000-;
6-10d after the planting, firstly adopting white light with the illumination intensity of 6000-;
after the 10 th day, white light with the illumination intensity of 5000-.
5. The photoautotrophic rooting method for sugarcane test-tube plantlets according to claim 4, characterized in that in the step (2), the disinfectant is a solution of thiophanate with a concentration of 15%.
6. The photoautotrophic rooting method for sugarcane test-tube plantlets according to claim 4, characterized in that in step (3), the planting substrate mainly comprises yellow mud, peat soil and calcium magnesium phosphate fertilizer, wherein the weight ratio of yellow mud to peat soil is 50 kg: 500 g: 100g of the raw materials are mixed; the planting tray is 6-9 holes in specification.
7. The photoautotrophic rooting method for sugarcane test-tube plantlets according to claim 4, characterized in that in step (4), the illumination device comprises four electrically telescopic vertical rods and two cross rods, wherein two ends of one cross rod are respectively connected with top ends of two electrically telescopic vertical rods, and two ends of the other cross rod are respectively connected with top ends of the other two electrically telescopic vertical rods; a plurality of connecting rods are arranged between the two cross rods at equal intervals, two ends of each connecting rod are respectively connected with the rod bodies of the two cross rods, a plurality of lamp holder groups are connected to the connecting rods at equal intervals, four lamp holders are arranged on the lamp holder groups at equal intervals, the four lamp holders are arranged along the length direction of the connecting rods, and a fluorescent lamp, a red light lamp, a blue light lamp and a near ultraviolet lamp are respectively arranged on the four lamp holders; still include control system, control system includes control operation panel, warning light and distance sensor, be equipped with controller and time-recorder in the control operation panel, the control operation panel upper surface is control panel, the warning light install in control panel is last, distance sensor install in the top of electronic flexible montant or on the connecting rod, the controller respectively with control panel the warning light the time-recorder distance sensor, four electronic flexible montant and four the lamp stand is connected.
8. An illumination device as claimed in claim 7.
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