CN110892097A - 用于制备核酸文库的组合物和方法 - Google Patents

用于制备核酸文库的组合物和方法 Download PDF

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Publication number
CN110892097A
CN110892097A CN201980002533.4A CN201980002533A CN110892097A CN 110892097 A CN110892097 A CN 110892097A CN 201980002533 A CN201980002533 A CN 201980002533A CN 110892097 A CN110892097 A CN 110892097A
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primer
sequence
linker
tail
kit
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English (en)
Chinese (zh)
Inventor
张之宏
郑涛
李冰思
邓望龙
汉雨生
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Guangzhou Burning Rock DX Co Ltd
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Guangzhou Burning Rock DX Co Ltd
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Priority to CN202110396910.6A priority Critical patent/CN113106145B/zh
Publication of CN110892097A publication Critical patent/CN110892097A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201980002533.4A 2018-04-03 2019-04-02 用于制备核酸文库的组合物和方法 Pending CN110892097A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110396910.6A CN113106145B (zh) 2018-04-03 2019-04-02 用于制备核酸文库的组合物和方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/CN2018/081748 WO2019191900A1 (en) 2018-04-03 2018-04-03 Compositions and methods for preparing nucleic acid libraries
CNPCT/CN2018/081748 2018-04-03
PCT/CN2019/081059 WO2019192489A1 (en) 2018-04-03 2019-04-02 Compositions and methods for preparing nucleic acid libraries

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CN110892097A true CN110892097A (zh) 2020-03-17

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CN202110396910.6A Active CN113106145B (zh) 2018-04-03 2019-04-02 用于制备核酸文库的组合物和方法

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US (2) US20210040475A1 (enExample)
EP (1) EP3740604A4 (enExample)
JP (2) JP2021517556A (enExample)
CN (2) CN110892097A (enExample)
AU (1) AU2019248276A1 (enExample)
BR (1) BR112020020207A2 (enExample)
CA (1) CA3095837A1 (enExample)
SG (1) SG11202009774XA (enExample)
WO (2) WO2019191900A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
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CN111455469A (zh) * 2020-04-07 2020-07-28 深圳易倍科华生物科技有限公司 一种单链快速建库方法及建库仪器
WO2023193456A1 (zh) * 2022-04-07 2023-10-12 广州燃石医学检验所有限公司 一种生物组合物、其制备方法及应用

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CN116829730A (zh) * 2020-11-10 2023-09-29 美国政府卫生与公众服务部 染色质占据和rna的单细胞分析测序
CN112538657B (zh) * 2020-12-25 2021-08-17 北京吉因加医学检验实验室有限公司 一种脑脊液基因测序建库、检测方法及其应用
CN113564226A (zh) * 2021-07-26 2021-10-29 深圳泰莱生物科技有限公司 一种捕获cfDNA5mC片段的检测方法
CN114736951B (zh) * 2022-04-20 2024-11-12 深圳大学 一种小分子rna的高通量测序文库构建方法
CN116287124A (zh) * 2023-05-24 2023-06-23 中国农业科学院农业基因组研究所 单链接头预连接方法、高通量测序文库的建库方法及试剂盒
WO2025072689A1 (en) * 2023-09-28 2025-04-03 The Regents Of The University Of California Methods and compositions for producing sequencing libraries
WO2025147442A1 (en) * 2024-01-02 2025-07-10 Grail, Inc. Rapid bisulfite conversion and recovery of nucleic acid molecules

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CA2439074A1 (en) 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
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EP2619327B1 (en) 2010-09-21 2014-10-22 Population Genetics Technologies LTD. Increasing confidence of allele calls with molecular counting
SG11201404243WA (en) * 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP2825675B1 (en) 2012-03-13 2017-12-27 Patel, Abhijit Ajit Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
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JP6998404B2 (ja) * 2017-08-01 2022-02-04 深▲セン▼恒特基因有限公司 標的ヌクレオチド配列の富化及び決定方法

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111455469A (zh) * 2020-04-07 2020-07-28 深圳易倍科华生物科技有限公司 一种单链快速建库方法及建库仪器
CN111455469B (zh) * 2020-04-07 2023-08-18 深圳易倍科华生物科技有限公司 一种单链快速建库方法及建库仪器
WO2023193456A1 (zh) * 2022-04-07 2023-10-12 广州燃石医学检验所有限公司 一种生物组合物、其制备方法及应用

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Publication number Publication date
SG11202009774XA (en) 2020-10-29
EP3740604A4 (en) 2021-12-29
JP2024096852A (ja) 2024-07-17
EP3740604A1 (en) 2020-11-25
CN113106145A (zh) 2021-07-13
CA3095837A1 (en) 2019-10-10
JP2021517556A (ja) 2021-07-26
US20210254051A1 (en) 2021-08-19
WO2019192489A1 (en) 2019-10-10
CN113106145B (zh) 2024-10-11
WO2019191900A1 (en) 2019-10-10
AU2019248276A1 (en) 2020-10-22
US12460202B2 (en) 2025-11-04
US20210040475A1 (en) 2021-02-11
BR112020020207A2 (pt) 2021-01-19

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