CN110876684A

CN110876684A - Preparation method of washing-free polyhexamethylene guanidine hydrochloride disinfectant

Info

Publication number: CN110876684A
Application number: CN201911200967.3A
Authority: CN
Inventors: 刘宏; 李传碧
Original assignee: Jilin Normal University
Current assignee: Jilin Normal University
Priority date: 2019-11-29
Filing date: 2019-11-29
Publication date: 2020-03-13

Abstract

The invention belongs to a preparation method of a washing-free polyhexamethylene guanidine hydrochloride disinfectant. The preparation method comprises dissolving 0.5-6 g polyhexamethylene guanidine hydrochloride in 92 g medical deionized water, adding 1.5-3 g coconut oil amide betaine (CAB) and 0.8-1.5 g alkyl glycoside (APG), adjusting pH value of the solution to 4.5-5.0 with sodium hydroxide, adding the above materials into a stirring tank, mixing well, and packaging. The disinfectant has broad-spectrum sterilization, quick action, long-lasting sterilization capability, safety, no toxicity and environmental protection. The antibacterial agent has a strong killing effect on brucella, escherichia coli, staphylococcus aureus, candida albicans and the like, is good in stability, has an effective period of two years, and has the advantages of easiness in smearing, rapidness in permeation, quickness in volatilization, no coloring, easiness in cleaning and the like; can be used for skin and wound surface disinfection, hand disinfection, air disinfection, gynecological and urological disinfection, drinking water disinfection and the like, and is a novel and ideal convenient disinfectant.

Description

Preparation method of washing-free polyhexamethylene guanidine hydrochloride disinfectant

Technical Field

The invention belongs to the field of disinfectants, and particularly relates to a preparation method of a washing-free polyhexamethylene guanidine hydrochloride disinfectant.

Background

In public places with relatively concentrated and crowded personnel, such as hospitals, airports, stations, restaurants, office places and the like, the spread of bacteria and viruses is very easy to cause public cross infection. Particularly, hospitals are not only sites for treating diseases but also sites that may become disease transmission sites. The transmission of these bacteria and viruses, especially due to the touch of the hands of the public, such as doctor's palpation of patients, handrails, door handles, handrails in the car, etc., is an important factor contributing to cross infection of the public. The implication of combining the prevalence of SARS with our knowledge, has led us to the recognition of the importance of large-scale prevention of disease in public areas. In this case, the disposable disinfectant is produced at the same time, but is only used in hospitals at present, and is not used in other public places.

Most of the no-clean disinfectants on the market at present adopt ethanol as a disinfection component. Firstly, the sterilization concentration of the ethanol needs to reach 75 percent to play a role, and the ethanol hurts hands; secondly, ethanol is resistant; thirdly, potential safety hazards exist in transportation of the ethanol raw material. The sterilizing capability is lost along with the volatilization of the ethanol! The ethanol is used in a large amount and can be volatilized for a long time. Ethanol, however, has strong side effects on the skin. The ethanol sterilization range is relatively wide. Besides ethanol, other disinfection components are as follows: the disinfectant can be divided into a sterilizing agent, a high-efficiency disinfectant, a middle-efficiency disinfectant and a low-efficiency disinfectant according to the action level. The sterilizing agent can kill all microorganisms to meet the sterilization requirement, and comprises formaldehyde, glutaraldehyde, ethylene oxide, peracetic acid, hydrogen peroxide, chlorine dioxide, chlorine, copper sulfate, quicklime and the like. These disinfecting ingredients have the following drawbacks: the aldehyde disinfectant has good sterilization effect, but has great harm to the environment. Is a type prohibited by the country. The peroxygen such as hydrogen peroxide, peroxyacetic acid and the like are disinfectants but have great corrosivity to the disinfected objects, and the application range is limited. How long they last to sterilize: the peroxide compound can kill bacteria while breaking off and has strong corrosion to the article to be killed. The sterilization range is large: the sterilization range of peroxy and aldehyde is wide, belonging to high-efficiency bactericide. In addition, the thickening agent glycerol is added into the no-clean disinfectant in the prior art, so that the disinfectant has high viscosity and poor hand feeling.

Brucellosis is a common disease in humans and animals, and is a chronic and contagious disease characterized by abortion caused by brucellosis infection. The previous special disinfectant for brucella is lysol, which is prohibited by the nation, and many disinfectant products in the market at present, such as 84 disinfectant, peroxyacetic acid and the like, can kill brucella. However, the special bactericide for the brucella has few types and poor killing effect, and no specific disinfectant exists at present.

Therefore, a disposable disinfectant which can be used in public places and can quickly and conveniently perform the hand disinfection effect is urgently needed to prevent cross infection.

Disclosure of Invention

The invention aims to provide a convenient, quick and wash-free preparation method of polyhexamethylene guanidine hydrochloride disinfectant, which has no drug resistance and can protect the skin while disinfecting.

Technical scheme of the invention

A method for preparing a washing-free polyhexamethylene guanidine hydrochloride disinfectant comprises the following steps,

dissolving 0.5-6 g of polyhexamethylene guanidine hydrochloride in 92 g of medical deionized water, adding 1.5-3 g of coconut oil amide betaine (CAB) and 0.8-1.5 g of alkyl glycoside (APG), adjusting the pH value of the solution to 4.5-5.0 by using sodium hydroxide, adding the raw materials into a stirring tank, uniformly mixing, and then subpackaging.

The optimum weight composition of the following ingredients was 2 grams of polyhexamethylene guanidine hydrochloride, 1.5 grams of cocamide betaine (CAB), 0.8 grams of alkyl glycoside (APG), 92 grams of medical deionized water, and the pH of the solution was adjusted to 5.0 with sodium hydroxide.

The invention has the advantages of

1. The invention provides a washing-free polyhexamethylene guanidine hydrochloride disinfectant and a preparation method thereof, which is a hydrophilic solution prepared by adding polyhexamethylene guanidine hydrochloride, cocamide betaine (CAB) and alkyl glycoside (APG) into medical deionized water and uniformly stirring at normal temperature. The disinfectant has broad-spectrum sterilization, quick action, long-lasting sterilization capability, safety, no toxicity and environmental protection. The antibacterial agent has a strong killing effect on brucella, escherichia coli, staphylococcus aureus, candida albicans and the like, is good in stability, has an effective period of two years, and has the advantages of easiness in smearing, rapidness in permeation, quickness in volatilization, no coloring, easiness in cleaning and the like; can be used for skin and wound surface disinfection, hand disinfection, air disinfection, gynecological and urological disinfection, drinking water disinfection and the like, and is a novel and ideal convenient disinfectant.

2. The disinfectant has the advantages of no washing, wide disinfection range, no effect on other disinfectants, and long action duration, and can disinfect brucella.

3. The polyhexamethylene guanidine hydrochloride does not have the problem of drug resistance, does not generate drug resistance even after being used for a long time, and does not hurt hands.

4. Because the coconut oil amide betaine is added, the disinfectant is in a foam shape after being sprayed out and is easy to volatilize, and the requirement of no-clean is met.

5. The alkyl glycoside is a thickening agent, and the existing technology is glycerol which has the advantages of high viscosity, difficult volatilization and poor hand feeling. The alkyl glycoside is easy to volatilize and has good hand feeling.

6. The application has the advantages that the alkyl glycoside and the cocoamidobetaine have synergistic effect, and the emulsifying effect is good. The emulsifier is cocoanut oil amide betaine; the alkyl glycoside has good compatibility, can be compounded with various ionic and nonionic surfactants to generate synergistic action, has good foamability, rich and fine foam, good solubility, strong alkali and electrolyte resistance, good thickening capability and good skin compatibility, obviously improves the mildness of the formula, is non-toxic, non-irritant, easy to biodegrade, non-irritant and good in antibacterial property; the alkyl glycoside also has very good moisturizing function.

Drawings

Fig. 1 is a bacteriostatic test chart of the disinfectant of the present application.

Detailed Description

Most of the washing-free disinfectants in the prior art are ethanol, and the ethanol can kill various bacteria, fungi and viruses and prevent the bacteria, fungi and viruses from immersing into cells of a human body. Although ethanol is used for surface disinfection in some places, due to the characteristics of strong fat solubility and strong protein denaturation of the ethanol, the ethanol can also cause damage to cells on the surface of the skin while disinfecting and cleaning hands, and causes uncomfortable reactions such as dry skin, itching and the like.

The invention provides a washing-free polyhexamethylene guanidine hydrochloride disinfectant and a preparation method thereof, which is a hydrophilic solution prepared by adding polyhexamethylene guanidine hydrochloride, cocamide betaine (CAB) and alkyl glycoside (APG) into medical deionized water and uniformly stirring at normal temperature. The invention mainly solves the technical problem of providing a convenient and quick washing-free disinfectant. The polyhexamethylene guanidine is adopted for sterilization and disinfection, and meanwhile, the alkyl glycoside moisturizing component is added, so that the skin can be protected when the purposes of disinfection and hand cleaning are achieved.

Example 1

The washing-free polyhexamethylene guanidine hydrochloride disinfectant has the preparation method that,

dissolving 3 g of polyhexamethylene guanidine hydrochloride in 92 g of medical deionized water, adding 1.5 g of coconut oil amide betaine (CAB) and 0.8 g of alkyl glycoside (APG), adjusting the pH value of the solution to 5.0 by using sodium hydroxide, adding the raw materials into a stirring tank, uniformly mixing, and subpackaging.

Example 2

The washed polyhexamethylene guanidine hydrochloride disinfectant has the preparation process including the steps of,

6 g of polyhexamethylene guanidine hydrochloride is dissolved in 92 g of medical deionized water, 3 g of coconut oil amide betaine (CAB) and 1.5 g of alkyl glycoside (APG) are added, the pH value of the solution is adjusted to 4.5 by sodium hydroxide, the raw materials are added into a stirring tank, and the mixture is uniformly mixed and then packaged.

Example 3

dissolving 0.5 g of polyhexamethylene guanidine hydrochloride in 92 g of medical deionized water, adding 2 g of coconut oil amide betaine (CAB) and 1.0 g of alkyl glycoside (APG), adjusting the pH value of the solution to 4.8 by using sodium hydroxide, adding the raw materials into a stirring tank, uniformly mixing, and subpackaging.

The raw materials are mixed evenly and then are subpackaged into 25 ml liquid spray bottles, which are convenient to carry and use. The filling process does not need to add any propellant. The operation steps are reduced, the production cost is reduced, and the sterilization efficiency is improved.

The disinfectant belongs to a cationic disinfection type, and can not be used together with anionic emulsifiers such as sodium stearate, lauric soap and the like so as to avoid reducing the sterilization effect.

Test report of no-clean polyhexamethylene guanidine hydrochloride disinfectant

Experimental drugs: antibacterial detection test of washing-free polyhexamethylene guanidine hydrochloride disinfectant drug

Experimental strains: escherichia coli, Staphylococcus aureus

The experimental process comprises the following steps:

1. weighing the polyhexamethylene guanidine hydrochloride medicaments, weighing the polyhexamethylene guanidine hydrochloride medicaments respectively, adding water to dissolve the polyhexamethylene guanidine hydrochloride medicaments, and preparing an experiment after the polyhexamethylene guanidine hydrochloride medicaments are completely dissolved.

2. Taking the culture medium for the experimental strains to perform the following steps of 1: 1000 and three 96-well plates were simultaneously taken. The diluted bacterial liquid is added into the front seven rows of 96-well plates, the addition amount of each row of each well is 150 microliters, and three 96-well plates are added in total.

3. 150 microliters of diluted bacteria liquid and 30 microliters of drugs are sequentially added into the first three rows of one hole of each 96-plate. The dilution was carried out sequentially backwards using a calandria, for a total of 24 concentration gradients. The seventh row of the well plate is a control of blank bacteria liquid.

4. 150 microliters of medium was added to each eighth row of the well plate as a blank.

5. After all the reagents are added, the 96-well plate is sealed by a sealing film, the information of the three drugs is respectively marked, and the three drugs are put into an incubator at 37 ℃ for culture.

6. After culturing for 2h, 4h, 6h, 8h and 10h, detecting by using a microplate reader and recording the bacteria liquid data (the detection wavelength is 630 nm).

Experimental data:

1. escherichia coli

The antibacterial detection chart of the medicine is shown in figure 1

From the experiments the following conclusions can be drawn:

from the above figure, it can be seen that the concentration of bacteria in the culture solution with the addition of the leave-on disinfectant is constant with the time. When the diluted concentration reaches 10 times, the bacteria grow, and the bactericidal capacity of the disinfectant is marked to be reduced.

The experiment was carried out simultaneously with a comparative experiment at different times, indicating that 10 times dilution is the sterilization limit.

Claims

1. A preparation method of a washing-free polyhexamethylene guanidine hydrochloride disinfectant is characterized by comprising the following steps:

dissolving 0.5-6 g of polyhexamethylene guanidine hydrochloride in 92 g of medical deionized water, adding 1.5-3 g of coconut oil amide betaine and 0.8-1.5 g of alkyl glycoside, adjusting the pH value of the solution to 4.5-5.0 by using sodium hydroxide, adding the raw materials into a stirring tank, uniformly mixing, and subpackaging.

2. The method for preparing the washing-free polyhexamethylene guanidine hydrochloride disinfectant as claimed in claim 1, wherein the method comprises the following steps: the optimum weight composition of the following components is 2 g of polyhexamethylene guanidine hydrochloride, 1.5 g of cocamide betaine, 0.8 g of alkyl glycoside and 92 g of medical deionized water, and the pH value of the solution is adjusted to 5.0 by sodium hydroxide.