CN110862935B - High-temperature-resistant bacillus Gongbenniang and high-temperature compost fermentation method - Google Patents
High-temperature-resistant bacillus Gongbenniang and high-temperature compost fermentation method Download PDFInfo
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Abstract
The invention relates to the field of microbial technology and environmental engineering, and discloses high-temperature-resistant longevity bacillus and a high-temperature compost fermentation method, wherein the high-temperature-resistant longevity bacillus is named as NH-1, the preservation number of the high-temperature-resistant longevity bacillus is CGMCC 17919, and the classified name of microorganisms is GongshobacillusBacillus hisashii. The strain can normally metabolize and grow at the temperature of 35-100 ℃, the pH value of 6-11 and the salinity of 0-5 percent, can grow by utilizing various carbon sources and nitrogen sources, and has a very broad substrate spectrum. The strain can be used for performing high-temperature aerobic composting treatment on a plurality of organic matters such as kitchen waste, agricultural product tailings and slaughterhouse waste by matching with the special high-temperature composting process, so that the organic matters are completely humified, dehydrated and dried, and the weight of the organic matters is reduced by 65-90%, and finally the organic fertilizer with excellent quality is formed.
Description
Technical Field
The invention relates to the field of microbial technology and environmental engineering, in particular to high-temperature-resistant bacillus gongyou and a high-temperature compost fermentation method.
Background
High-temperature composting is a procedural noun, in particular to a method for treating garbage, and the high-temperature composting technology is a process for biologically degrading organic matters capable of being decomposed in the garbage by using microorganisms under certain external conditions to enable the organic matters to become substances similar to fertile humus soil. The high-temperature composting mode has certain characteristics on garbage, and organic garbage is a precondition for microorganisms to live and propagate. High-temperature composting is classified into aerobic high-temperature composting technology and anaerobic high-temperature composting technology according to the preference and life habits of different microorganisms. Among them, the aerobic high-temperature composting technology is the main development direction, because the aerobic high-temperature composting technology has the following advantages for the anaerobic high-temperature composting technology: firstly, the degradation of the aerobic high-temperature composting technology is sufficient, the organic matters which can be degraded by the aerobic high-temperature composting technology are far higher than those of the anaerobic high-temperature composting technology in the same time, and the aerobic high-temperature composting technology can degrade the organic matters more quickly and more thoroughly on the premise of degrading the same amount of organic matters. Meanwhile, the aerobic high-temperature composting technology also has the following characteristics: the aerobic high-temperature compost can release a large amount of heat in the fermentation process, the reactor body is always kept in a relatively high-temperature state, the high temperature can promote the further acceleration of the fermentation process, and meanwhile, a part of bacteria and viruses can be killed, so that the harmless treatment of garbage is facilitated. In addition, the aerobic high-temperature composting technology is beneficial to mechanized operation, and the advantages establish the status of the aerobic high-temperature composting technology in the high-temperature composting field of China.
The maximum temperature of the conventional aerobic fermentation is generally maintained at 50-70 ℃, so the aerobic fermentation technology is also called high-temperature (aerobic) composting. The temperature is an important factor influencing the aerobic fermentation process, and the whole fermentation process can be divided into 4 stages according to the temperature: low temperature stage, heating stage, high temperature stage and cooling stage. Traditional composting practice considers that the composting fermentation temperature cannot be higher than 70 ℃, otherwise, functional microorganisms die in a large amount, the fermentation decomposition process is slowed down, and the fermentation is finally incomplete.
In recent years, researches show that increasing the temperature of a compost can accelerate the humification process and shorten the fermentation period.
Disclosure of Invention
In order to solve the technical problems, the invention provides high-temperature resistant Bacillus longevity and a high-temperature compost fermentation method,
the specific technical scheme of the invention is as follows: the invention provides high-temperature-resistant Podospora gondii, which is named as NH-1, is preserved in China general microbiological culture Collection center (CGMCC) in 6, 12 and 2019, has a preservation number of CGMCC 17919, and is named as Podospora gondii in a microorganism classification wayBacillus hisashii(ii) a The gene sequence of the 16s ribosome subunit of the NH-1 is shown in SEQ ID NO. 1.
The strain is a single facultative aerobic Bacillus palaestis strain HN-1 separated from hot spring high-temperature hot spring environment of volcanic rock mine of southern Hainan lake, and the strain with the most similar 16s ribosome subunit gene sequence of the HN-1 strain is Bacillus palaestis (Bacillus (Gongshoyu) ((China) through sequencing identificationBacillus hisashii). The characteristics are as follows:
(1) and screening and subculturing by using a TM culture medium. (Na is contained per liter of TM medium:. RTM.)2HPO4·12H2O 0.43g、KH2PO4 54.0mg、MgCl2·6H2O 0.2g、CaSO4·2H2O40.0 mg, 0.5mL of 0.01M ferric citrate solution, 1.0mL of trace element solution, 0.1g of nitrilotriacetic acid, 2.5g of tryptone, 2.5g of yeast extract, 15g of agar (prepared with solid culture medium) and pH7.2, wherein each liter of the 1000-fold trace element solution contains: cocl (cobalt carbide)2·6H2O 45.0mg、Na2MoO4·2H2O 25.0mg、CuSO4·5H2O 25.0mg、H3BO3 0.5g、ZnSO4·7H2O 0.5g、MnSO4·H2O 2.28g、Srcl2 34.0mg、Na2SiO34.0mg, NaF 2.4mg, concentrated sulfuric acid 0.5 mL)
(2) Strain HN-1 required growth conditions in TM medium: the temperature is 35-100 deg.C (most suitable for 75 deg.C), pH is 6-11 (most suitable for 8.5), and salinity is 0-5% (w/v, most suitable for 1%).
(3) And the strain HN-1 is gram-positive bacillus, has the size of 0.5-1 multiplied by 2-5 (mu m) and has no flagella.
(4) The generation time of strain HN-1 in TM medium was about 0.6 h.
(5) And the bacterial strain HN-1 is sensitive to various antibiotics such as penicillin, vancomycin, streptomycin, erythromycin, chloramphenicol, kanamycin, neomycin and the like.
(6) The strain HN-1 mainly contains saturated fatty acid C13:0、C14:0、C15:0。
(7) The strain HN-1 has alkaline phosphatase activity.
(8) And the methyl red test and the hydrogen sulfide production test of the strain HN-1 are positive.
(9) The indole-producing test, the V-P test, the catalytic activity and the oxidase activity of the strain HN-1 were negative.
As described in the background art, the maximum temperature of the conventional aerobic fermentation is generally maintained at 50-70 ℃, and when the compost fermentation temperature is higher than 70 ℃, the fresh functional microorganisms can live or keep high activity. For this reason, the present inventors found that the Bacillus palaestis NH-1 of the present invention can survive and maintain high activity in an environment of 35-100 ℃ temperature (most suitable for 75 ℃), pH 6-11 (most suitable for 8.5), and salinity 0-5% (w/v, most suitable for 1%) during screening. The high-temperature composting by adopting the strain can improve the temperature of a compost, accelerate the humification process and shorten the fermentation period.
Preferably, the high-temperature-resistant brevibacillus uteri NH-1 can also be a culture of the brevibacillus uteri NH-1 or a culture after passage.
The invention also provides a mutant of the high-temperature-resistant longevity bacillus, which is obtained by carrying out mutagenesis, domestication, gene recombination or natural mutation on the longevity bacillus NH-1.
The invention also provides a thallus culture containing the high-temperature-resistant Gongshobacillus or the mutant.
The invention also provides a bacterial culture which is a bacterial liquid, a microbial inoculum or activated sludge and the like.
The invention also provides an enzyme preparation or single-cell protein containing the high-temperature-resistant brevibacillus cervicalis or the mutant.
The invention also discloses application of the high-temperature-resistant Gongshou bacillus or the mutant in sewage treatment, garbage treatment, composting, enzyme preparation production and single-cell protein production.
The invention also provides a high-temperature compost fermentation method, which comprises the following steps:
1) and (4) pretreating the first batch of materials.
2) And (3) putting strains: and fully mixing the fermentation liquor obtained after the fermentation of the high-temperature-resistant Gongshou bacillus or the mutant is completed with wood chips, drying at 55-65 ℃ to form a microbial inoculum, and putting the microbial inoculum into the first batch of materials.
3) And (4) heating the materials.
4) High-temperature fermentation: the fermentation temperature is 70-100 ℃.
5) And (5) semi-continuously feeding and discharging.
Preferably, step 1) is specifically: cutting and crushing an original organic garbage material as a first batch of material into fragments with the diameter of 3-7cm, mixing the fragments with quicklime with the mass of 2-5%, and controlling the water content of the obtained mixture to be 75-85%; 0.5-1.0g of alkaline amylase, 0.2-0.6g of alkaline protease and 0.4-1.2g of esterase are added into each ton of original organic garbage material, fully mixed and kept stand for 0.5-1 h.
The invention adopts special pretreatment means such as physical cutting, heating, chemical hydrolysis, enzymatic hydrolysis and the like to quickly heat the compost to over 50 ℃, so that the compost quickly enters a high-temperature stage, HN-1 strains quickly become absolute dominant strains in a compost system, and on the basis of the absolute dominant strains, a continuous compost fermentation process capable of feeding and discharging materials every day is realized.
In the pretreatment of the present invention, the reason for the physical cutting is that the smaller the particle size of the material, the more easily it is decomposed, but considering that while the surface area of the material is increased, a certain degree of porosity must be maintained in order to allow ventilation so that the material can obtain a sufficient supply of oxygen, the organic material is cut and pulverized here into pieces of 3 to 7 cm. 2-5% of quicklime is added into the material, and the quicklime can react with excessive moisture in the material to release heat, so that the material is rapidly heated, and simultaneously, the moisture content of the material can be controlled at the optimal reaction humidity of 75-85%. The alkaline amylase, alkaline protease and esterase added into the material can assist in hydrolyzing starch, protein and lipid substances in the material into small molecules which are easy to be utilized by high-temperature strains in advance, so that the compost can enter a high-temperature stage quickly.
Preferably, in the step 2), the volume ratio of the fermentation liquor to the wood chips is 1: 0.8-1.2; the addition amount of the microbial inoculum is at least 30 percent of the weight of the first batch of materials.
Preferably, step 3) is specifically: heating the first batch of materials and the microbial inoculum to above 40 ℃ within 1h, maintaining the heating for 2-4h, and stopping heating after the temperature rises to 55-65 ℃.
Preferably, in the step 4), the humidity of the air in the fermentation environment is controlled to be 85-90%, and if the humidity is insufficient, water is supplemented; the high-temperature fermentation time is 48-72 h.
After the microbial inoculum is activated at the temperature of 40 ℃, the microbial inoculum rapidly breeds and decomposes organic matters to generate a large amount of heat, so that the temperature of materials is raised to 70-100 ℃, and the high temperature can be maintained until the materials are humified and most of water is gasified. After fermentation is completed, the weight of the material can be reduced by 65-90%. In the fermentation process, the equipment controls the air humidity in the fermentation container to be 85-90%, and if the humidity is insufficient, the equipment replenishes water by spraying water. HN-1 bacteria can inhibit growth of harmful bacteria, and does not produce odor such as malodor and rancidness.
Preferably, the step 5) is specifically as follows: completing humification after the high-temperature fermentation in the step 4), and starting discharging and feeding; wherein the discharging proportion is not more than 20 wt%; then adding new organic garbage materials which are not pretreated, wherein the adding amount is not more than 20wt% of the materials obtained in the step 4); and (4) performing secondary discharging and material supplementing within 18-24 hours after the new organic garbage material is supplemented, and performing semi-continuous feeding and discharging according to repeated operation.
Preferably, each batch of the microbial inoculum is fed to meet the requirement of 15-30 times of discharging feeding operation, and then the fermentation period is restarted according to the steps 2) -5).
Compared with the prior art, the invention has the beneficial effects that:
1) the strain can normally metabolize and grow at the temperature of 35-100 ℃, the pH value of 6-11 and the salinity of 0-5% (w/v), various monosaccharides, oligosaccharides, polysaccharides, organic acids and alcohols can be used as carbon sources, various composite nitrogen sources such as tryptone, fish peptone and beef extract can be used for growing, and the substrate spectrum is very wide.
2) The strain can be used for performing high-temperature aerobic composting treatment on a plurality of organic matters such as kitchen waste, agricultural product tailings and slaughterhouse waste by matching with the special high-temperature composting process, so that the organic matters are completely humified, dehydrated and dried, and reduced in weight by 65-90%, and finally the organic fertilizer with excellent quality is formed.
3) The high-temperature composting process also relates to special pretreatment means such as physical cutting, heating, chemical hydrolysis, enzymatic hydrolysis and the like, so that the compost can be heated quickly to be higher than 50 ℃, the high-temperature composting stage is quickly started, HN-1 strains are quickly changed into absolute dominant strains in a compost system, and on the basis, the continuous composting fermentation process capable of feeding and discharging materials every day is realized.
Drawings
FIG. 1 is a graph showing the growth of Bacillus gongyuensis NH-1 at different temperatures.
Detailed Description
The present invention will be further described with reference to the following examples.
General examples
The high-temperature-resistant palace-longevity bacillus is named as NH-1, is preserved in China general microbiological culture Collection center (CGMCC) in 6, 12 and 2019 in 12 months, has the preservation number of CGMCC 17919, and is classified and named as palace-longevity bacillusBacillus hisashii(ii) a The gene sequence of the 16s ribosome subunit of the NH-1 is shown in SEQ ID NO. 1.
Preferably, the high-temperature-resistant brevibacillus uteri NH-1 can also be a culture of the brevibacillus uteri NH-1 or a culture after passage.
A mutant of high-temperature-resistant Gongshou bacillus is obtained by carrying out mutagenesis, domestication, gene recombination or natural mutation on the Gongshou bacillus NH-1.
A cell culture containing the high-temperature-resistant Bacillus palaestis or the mutant is provided.
A bacterial culture is a bacterial liquid, a bacterial agent or activated sludge.
An enzyme preparation or single-cell protein containing the high-temperature-resistant brevibacillus cervi or the mutant.
The invention also discloses application of the high-temperature-resistant Gongshou bacillus or the mutant in sewage treatment, garbage treatment, composting, enzyme preparation production and single-cell protein production.
The growth curves of the strains of the invention at different temperatures (70 ℃, 80 ℃, 90 ℃, 100 ℃) are shown in FIG. 1. As can be seen from the growth curve of NH-1 at 70 ℃, 80 ℃, 90 ℃ and 100 ℃, the metabolic activity of the high-temperature strain is slightly reduced along with the temperature rise at 70-90 ℃, but the overall metabolic activity is better, and the activity at 100 ℃ can still reach 75% of the activity at 70 ℃.
A high-temperature compost fermentation method comprises the following steps:
1) pretreatment of first batch of materials: cutting and crushing an original organic garbage material as a first batch of material into fragments with the diameter of 3-7cm, mixing the fragments with quicklime with the mass of 2-5%, and controlling the water content of the obtained mixture to be 75-85%; 0.5-1.0g of alkaline amylase, 0.2-0.6g of alkaline protease and 0.4-1.2g of esterase are added into each ton of original organic garbage material, fully mixed and kept stand for 0.5-1 h.
2) And (3) putting strains: and (3) fully mixing the fermentation liquor obtained after the fermentation of the high-temperature-resistant Gongshou bacillus or the mutant is completed with wood chips, drying at 55-65 ℃ to form a microbial inoculum, and putting the microbial inoculum into the first batch of materials. Wherein the volume ratio of the fermentation liquor to the wood chips is 1: 0.8-1.2; the addition amount of the microbial inoculum is at least 30 percent of the weight of the first batch of materials.
3) Heating materials: putting the first batch of materials and strains into a fermentation device with electric heating, stirring, ventilating and water replenishing functions, heating the materials to be above 40 ℃ within 1 hour by adopting an electric heating mode, maintaining the heating for 2-4 hours, and stopping the electric heating after the temperature of the materials is raised to 55-65 ℃.
4) High-temperature fermentation: the fermentation temperature is 70-100 ℃, the air humidity of the fermentation environment is controlled at 85-90%, and if the humidity is insufficient, water is supplemented; the high-temperature fermentation time is 48-72 h.
5) Semi-continuous feeding and discharging: completing humification after the high-temperature fermentation in the step 4), and starting discharging and feeding; the discharging proportion is not more than 20%, then new organic garbage materials are put in, the new materials can be directly put in without pretreatment or water proportion control, and the putting proportion is not more than 20% of the original weight of the fertilizer pile. And the material can be discharged and supplemented for the second time within 18-24h after the new material is supplemented, and the semi-continuous fermentation can be realized by repeated operation. Wherein, after each batch of microbial inoculum is added, the requirement of 15-30 times of discharging and feeding operations can be met, and then the fermentation period is restarted according to the steps 2) -5).
Example 1: kitchen waste composting treatment
Adding 5 tons of kitchen waste into a fermentation device with cutting and stirring functions to continuously cut and stir the kitchen waste into fragments of about 5cm, adding 3% of quicklime, controlling the water content of the materials to be 80%, adding 2.5g of alkaline amylase, 1g of alkaline protease and 2.5g of esterase after uniformly mixing, continuously mixing and stirring, starting electric heating, heating to 35 ℃, and maintaining for 1 hour. 2 tons of HN-1 microbial inoculum wood chips are added, the volume ratio of the liquid microbial inoculum to the dry wood chips is 1: 1.1 during preparation, the mixture is fully stirred, the electric heating power is increased, the temperature is raised to 45 ℃, and high-temperature composting is started. After starting for 4 hours, the temperature of the fertilizer pile is increased to 65 ℃, the temperature is gradually increased within 24 hours of starting, finally, the temperature reaches 85-100 ℃, and the humidity is gradually reduced from the initial 80 percent to about 50 percent. The temperature then slowly dropped, falling back to 65 ℃ within 48 hours of start-up, at which time 20% by volume of old material was discharged, and 1 ton of fresh material was replenished and fermentation continued. And continuously increasing the temperature of the fertilizer pile, maintaining the temperature between 70 and 100 ℃ according to the organic matter content condition of fed materials, and discharging and feeding materials in the same way again after feeding for 24 hours. After discharging and feeding for 10 times, 2.3 tons of organic fertilizer are obtained in total, the water content is 35 percent, and the weight of the material is reduced by 84.7 percent.
Example 2: solid waste compost for cultivation
Adding 4% of quicklime into 10 tons of pig farm manure, uniformly stirring, controlling the water content of the material to be 82%, adding 5.5g of alkaline amylase, 5.0g of alkaline protease and 10g of esterase, continuously mixing and stirring, starting electric heating, heating to 40 ℃, and maintaining for 1 hour. 4 tons of HN-1 microbial inoculum wood chips are added, the volume ratio of the liquid microbial inoculum to the dry wood chips is 1:0.8 during preparation, the mixture is fully stirred, the electric heating power is increased, the temperature is raised to 45 ℃, and high-temperature composting is started. After starting for 4 hours, the temperature of the fertilizer pile is increased to 65 ℃, the temperature is gradually increased within 24 hours of starting, finally, the temperature reaches 85-100 ℃, and the humidity is gradually reduced from the initial 80 percent to about 55 percent. The temperature then slowly dropped, falling back to 65 ℃ within 48 hours of start-up, at which time 20% by volume of old material was discharged, and 2 tons of fresh material were replenished and fermentation continued. And continuously increasing the temperature of the fertilizer pile, maintaining the temperature between 70 and 100 ℃ according to the organic matter content condition of fed materials, and discharging and feeding materials in the same way again after feeding for 24 hours. After discharging and feeding for 10 times, the total amount of the obtained organic fertilizer is 4.5 tons, the water content is 38 percent, and the weight of the material is reduced by 85.0 percent.
Example 3: compost of agricultural and sideline products
Taking 5 tons of straws, adding the straws into a fermentation device with cutting and stirring functions, continuously cutting and stirring the straws into fragments of about 6cm, adding 3 percent of quicklime, uniformly stirring the mixture, controlling the water content of the materials to be 85 percent, adding 3.0g of alkaline amylase, 2.5g of alkaline protease and 4.0g of esterase, continuously mixing and stirring the mixture, starting electric heating, raising the temperature to 40 ℃, and maintaining the temperature for 2 hours. 2.5 tons of HN-1 microbial inoculum wood chips are added (during preparation, the volume ratio of the liquid microbial inoculum to the dry wood chips is 1: 1.2), the mixture is fully stirred, the electric heating power is increased, the temperature is raised to 45 ℃, and high-temperature composting is started. After starting for 6h, the temperature of the fertilizer pile is increased to 65 ℃, the temperature is gradually increased within 24 hours of starting, and finally reaches 85-100 ℃, and the humidity is gradually reduced from the initial 85 percent to about 55 percent. The temperature was then slowly reduced and dropped back to 65 ℃ over 60 hours of start-up, at which time 25% of the volume of old material was discharged and 1.25 tonnes of fresh material was replenished and fermentation continued. And continuously increasing the temperature of the fertilizer pile, maintaining the temperature between 70 and 100 ℃ according to the organic matter content condition of fed materials, and discharging and feeding materials in the same way again after feeding for 24 hours. After discharging and feeding for 10 times, 2.78 tons of organic fertilizer are obtained totally, the water content is 35 percent, and the weight of the material is reduced by 84.1 percent.
The raw materials and equipment used in the invention are common raw materials and equipment in the field if not specified; the methods used in the present invention are conventional in the art unless otherwise specified.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and all simple modifications, alterations and equivalents of the above embodiments according to the technical spirit of the present invention are still within the protection scope of the technical solution of the present invention.
Sequence listing
<110> Zhejiang Jiahe green environmental science and technology Co Ltd
<120> high-temperature-resistant bacillus palaestis and high-temperature compost fermentation method
<130> 2019
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1473
<212> DNA
<213> Bacillus hysterophorus NH-1(Bacillus hisashii HN-1)
<400> 1
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ttttgttggt tagcggcgga cgggtgagta acacgtgggt aacctgcctg taagaccggg 120
ataactccgg gaaaccggtg ctaataccgg atagattatc tttccgcctg gagagataag 180
gaaagatggc tattgccatc acttacagat gggcccgcgg cgcattagct agttggtgag 240
gtaacggctc accaaggcga cgatgcgtag ccgacctgag agggtgatcg gccacactgg 300
gactgagaca cggcccagac tcctacggga ggcagcagta gggaatcttc cgcaatggac 360
gaaagtctga cggagcaacg ccgcgtgagc gaagaaggtc ttcggatcgt aaagctctgt 420
tgttagggaa gaacaagtat cggaggaaat gccggtacct tgacggtacc tgacgagaaa 480
gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc gttgtccgga 540
tttattgggc gtaaagcgcg cgcaggcggt cctttaagtc tgatgtgaaa tcttgcggct 600
caaccgcaag cggtcattgg aaactggggg acttgagtgc agaagaggaa agcggaattc 660
cacgtgtagc ggtgaaatgc gtagagatgt ggaggaacac cagtggcgaa ggcggctttc 720
tggtctgtaa ctgacgctga ggcgcgaaag cgtggggagc aaacaggatt agataccctg 780
gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc ttcagtgctg 840
cagctaacgc attaagcact ccgcctgggg agtacggtcg caagactgaa actcaaagga 900
attgacgggg cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac 960
cttaccaggt cttgacatct cctgaccgcc ctggagacag ggtcttccct tcggggacag 1020
gatgacaggt ggtgcatgga tgtcgtcagc tcgtgtcgtg agatgttggc gttaagtccc 1080
gcaacgagcg caacccttgg ttctagttgc cagcattcag ttgggcactc tagagggact 1140
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ccgcccgtca caccacgaga gtttgtaaca cccgaagtcg gtgaggtaac cgcaaggagc 1440
cagccgccga aggtgggaca gatgattggg gtg 1473
Claims (8)
1. A high temperature resistant Bacillus palaestis is characterized in that: root of uterus and longevity budBacillus (A), (B), (C)Bacillus hisashii) NH-1 is preserved in China general microbiological culture Collection center (CGMCC) on 12.6.2019 with the preservation number of CGMCC No. 17919.
2. A bacterial culture comprising the thermophilic Auricularia palaestina strain of claim 1.
3. The microbial culture according to claim 2, which is a bacterial solution, a microbial inoculum or an activated sludge.
4. An enzyme preparation comprising the refractory Bacillus macerans of claim 1.
5. The use of the high temperature resistant Bacillus uterochrous of claim 1 in organic waste treatment, composting, enzyme preparation production, single cell protein production.
6. A high-temperature compost fermentation method is characterized by comprising the following steps:
1) preprocessing a first batch of materials;
2) and (3) putting strains: fully mixing fermentation liquor obtained after the fermentation of the high-temperature-resistant Gongshou bacillus in claim 1 with wood chips, drying at 55-65 ℃ to form a microbial inoculum, and putting the microbial inoculum into a first batch of materials;
3) heating the materials;
4) high-temperature fermentation: the fermentation temperature is 70-100 ℃;
5) and (5) semi-continuously feeding and discharging.
7. A high temperature compost fermentation process as claimed in claim 6 wherein:
the step 1) is specifically as follows: cutting and crushing an original organic garbage material as a first batch of material into fragments with the diameter of 3-7cm, mixing the fragments with quicklime with the mass of 2-5%, and controlling the water content of the obtained mixture to be 75-85%; adding 0.5-1.0g of alkaline amylase, 0.2-0.6g of alkaline protease and 0.4-1.2g of esterase into each ton of original organic garbage material, fully mixing, and standing for 0.5-1 h; and/or
In the step 2), the volume ratio of the fermentation liquor to the wood chips is 1: 0.8-1.2; the addition amount of the microbial inoculum is at least 30% of the weight of the first batch of materials;
the step 3) is specifically as follows: heating the first batch of materials and the microbial inoculum to above 40 ℃ within 1h, maintaining the heating for 2-4h, and stopping heating after the temperature rises to 55-65 ℃; and/or
In the step 4), the air humidity of the fermentation environment is controlled to be 85-90%, and if the air humidity is insufficient, water is supplemented; the high-temperature fermentation time is 48-72 h; and/or
The step 5) is specifically as follows: completing humification after the high-temperature fermentation in the step 4), and starting discharging and feeding; wherein the discharging proportion is not more than 20 wt%; then adding new organic garbage materials which are not pretreated, wherein the adding amount is not more than 20wt% of the materials obtained in the step 4); and (4) performing secondary discharging and material supplementing within 18-24 hours after the new organic garbage material is supplemented, and performing semi-continuous feeding and discharging according to repeated operation.
8. A high temperature composting fermentation process as claimed in claim 7 wherein: and adding the microbial inoculum again after 15-30 times of discharging and feeding operations.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018216818A1 (en) * | 2017-05-26 | 2018-11-29 | 日環科学株式会社 | Muscle quality improvement agent |
| CN109337904A (en) * | 2018-11-02 | 2019-02-15 | 中国科学院动物研究所 | Genome editing system and method based on C2c1 nuclease |
| CN109370944A (en) * | 2018-11-20 | 2019-02-22 | 江南大学 | One plant of bacillus for producing protease and its application in compost |
| CN109402014A (en) * | 2018-11-20 | 2019-03-01 | 江南大学 | A kind of bacillus of cellulase-producing and its application |
-
2019
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018216818A1 (en) * | 2017-05-26 | 2018-11-29 | 日環科学株式会社 | Muscle quality improvement agent |
| CN109337904A (en) * | 2018-11-02 | 2019-02-15 | 中国科学院动物研究所 | Genome editing system and method based on C2c1 nuclease |
| CN109370944A (en) * | 2018-11-20 | 2019-02-22 | 江南大学 | One plant of bacillus for producing protease and its application in compost |
| CN109402014A (en) * | 2018-11-20 | 2019-03-01 | 江南大学 | A kind of bacillus of cellulase-producing and its application |
Non-Patent Citations (2)
| Title |
|---|
| Bacillus hisashii sp. nov., isolated from the caeca of gnotobiotic mice fed with thermophile-fermented compost;Ayaka Nishida;《Int J Syst Evol Microbio》;20150812;第65卷(第11期);第56-63页 * |
| 利用微生物和高性能生物发酵;宫本浩邦;《生物工学会志》;20181231;第96卷(第2期);第3944-3949页 * |
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