CN110823875A - Chemiluminescence analyzer and reaction device thereof, and magnetic shaking device - Google Patents
Chemiluminescence analyzer and reaction device thereof, and magnetic shaking device Download PDFInfo
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- CN110823875A CN110823875A CN201911072866.2A CN201911072866A CN110823875A CN 110823875 A CN110823875 A CN 110823875A CN 201911072866 A CN201911072866 A CN 201911072866A CN 110823875 A CN110823875 A CN 110823875A
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- reaction
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/28—Moving reactors, e.g. rotary drums
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
Abstract
The application relates to a magnetic force shakes even device for chemiluminescence analysis appearance reaction unit, including a set of solenoid and the control circuit who corresponds, solenoid evenly sets up the periphery in each reaction cup in chemiluminescence analysis appearance reaction unit, control circuit connects the both ends at corresponding solenoid for the solenoid that the control corresponds changes the current direction. According to the invention, by changing the current direction of the electromagnetic coil, the reactants are mixed more uniformly, the mixed liquid is prevented from splashing on the cup wall, and the test result is more accurate and reliable.
Description
Technical Field
The application belongs to the technical field of medical instruments, and particularly relates to a chemiluminescence analyzer and a reaction device and a magnetic shaking device thereof.
Background
The diagnosis method, in vitro diagnosis, which is the most commonly used method in the medical field at present, refers to collecting body fluid, excrement and secretion of a human body to perform chemical composition or chemical reaction analysis so as to judge human body pathological changes. Such as chemiluminescence analysis and molecular diagnosis. The diagnosis methods adopt automatic or semi-automatic instruments to sample and analyze, and give a diagnosis report.
The reaction stage of chemiluminescence immunoassay is an important stage, and whether reagents, magnetic beads and samples react sufficiently or not determines whether the value measured by the instrument can correctly reflect the current state of a patient or not. In the reaction stage, the method mainly used in the current stage is mechanical mixing, the cup-connected reaction cups are moved back and forth by the reaction device and are mixed uniformly, the single-hole reaction cup is shaken by the motor and is mixed uniformly, and the mechanical mixing has the risk of splashing the mixed liquid onto the cup wall to cause inaccurate results.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: in order to solve the problem that the mixed liquid is easy to splash to the cup wall when the mixed liquid in the reaction cup is shaken up in the prior art, so that the detection and analysis results are inaccurate, the chemiluminescence analyzer, the reaction device thereof and the magnetic shaking up device are provided.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the invention provides a magnetic shaking device for a reaction device of a chemiluminescence analyzer, which comprises a group of electromagnetic coils and a control circuit for controlling the on-off of each electromagnetic coil, wherein the group of electromagnetic coils are respectively arranged at two sides of each reaction cup in the reaction device of the chemiluminescence analyzer;
the control circuit comprises a microcontroller and switch circuits connected in series in the energizing loops of the electromagnetic coils, and the microcontroller is used for controlling the alternate on-off of the switch circuits corresponding to the electromagnetic coils on the two sides of the reaction cup.
Further, according to the magnetic shaking device provided by the first aspect of the present invention, the central position of each electromagnetic coil faces the nanometer-scale magnetic particle gathering area in the reaction cup.
As a possible implementation, in the magnetic shaking-up device provided in the first aspect of the present invention, the switch circuit includes an optical coupler and an MOS circuit, the MOS circuit includes an MOS transistor, an electrical signal output end of the optical coupler is connected to a control end of the MOS transistor, and an electrical input end of the optical coupler is connected to a signal output end of the microcontroller.
Further, as a possible embodiment, the magnetic shaking apparatus provided by the first aspect of the present invention, one switch port of the MOS transistor is connected to ground, and the other switch port is connected to the electromagnetic coil; the optocoupler comprises a light emitting diode, the anode of the light emitting diode is connected with the anode of the power supply, and the cathode of the light emitting diode is connected with the signal output end of the microcontroller.
In another possible embodiment, the magnetic shaking apparatus provided by the first aspect of the present invention, the optical coupler includes a light emitting diode, an anode of the light emitting diode is connected to the signal output terminal of the microcontroller, and a cathode of the light emitting diode is connected to ground.
Furthermore, the magnetic shaking device provided by the first aspect of the invention further comprises a heat dissipation device, wherein the heat dissipation device is used for cooling the reaction device for the chemiluminescence analyzer.
The invention provides a reaction device of a chemiluminescence analyzer, which comprises the magnetic shaking-up device.
In a third aspect, the present invention provides a chemiluminescent analyzer comprising the reaction apparatus described above.
The invention has the beneficial effects that: electromagnetic coils are symmetrically arranged on two sides of each reaction cup on the reaction device in an electromagnetic generating mode, and in a reagent reaction stage, the electromagnetic coils on two sides of the reaction cups are alternately controlled by a microcontroller to be electrified, so that magnetic beads continuously run in the reaction cups, reactants are fully reacted by continuous motion of the magnetic beads, the mixing is uniform, the test and analysis results are more reliable, and meanwhile, the mixed liquid is prevented from splashing on the cup walls.
Drawings
The technical solution of the present application is further explained below with reference to the drawings and the embodiments.
FIG. 1 is a schematic structural diagram of an embodiment of the present application;
FIG. 2 is a schematic diagram of a control circuit of an embodiment of the present application;
fig. 3 is a schematic diagram of a control circuit according to another embodiment of the present application.
In the figure, 1 is an electromagnetic coil, 2 is a reaction cup, and 3 is a nano-scale magnetic particle gathering area in the reaction cup
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict.
The technical solutions of the present application will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
The electromagnetic coils are symmetrically arranged on two sides of each reaction cup on the reaction device in an electromagnetic generating mode, and the electromagnetic coils on two sides of the reaction cups are alternately controlled by the microcontroller to be electrified in a reagent reaction stage, so that the nano-scale magnetic particles continuously run in the reaction cups, and reactants are fully reacted.
Example 1:
this example 1 provides a magnetic shaking device for a reaction device of a chemiluminescence analyzer, which is shown in fig. 1 and comprises electromagnetic wires 1 arranged at two sides of each reaction cup 2 of the reaction device and a control circuit for controlling the electromagnetic coils at two sides to be alternatively electrified. The electromagnetic coil is positioned at the middle lower part of the reaction cup 2, and the central position of the electromagnetic coil is over against the nano-scale magnetic particle gathering area 3 in the reaction cup.
The control circuit includes an MCU and a switching circuit connected to a corresponding solenoid coil energizing circuit, as shown in fig. 2, the switching circuit of this embodiment includes an optocoupler U1 and an MOS transistor Q1, the optocoupler of this embodiment employs a TLP785 optocoupler, and the MOS transistor Q1 employs an N communication MOS transistor. The TLP785 comprises a light emitting diode and a phototriode, wherein the anode of the light emitting diode is connected with a power supply end, the cathode of the light emitting diode is connected with the signal output end of the MCU, and the signal output end of the MCU outputs a driving signal SIG1 of the switching circuit. The collector of the phototriode is connected with the positive electrode of a 24V power supply, the emitter of the phototriode is connected with the grid electrode of the MOS tube Q1, the source electrode of the Q1 is grounded, and the drain electrode of the Q1 is connected to the electromagnetic coil.
According to fig. 2, when the driving signal SIG1 output by the MCU is a low level signal, the light emitting diode in the optocoupler is turned on, the phototransistor outputs an electrical signal to the gate of the MOS transistor Q1 to control the conduction of the MOS transistor Q1, so that the switching circuit is turned on and the corresponding electromagnetic coil is energized; when the driving signal SIG1 output by the MCU is a high-level signal, the light emitting diode in the optical coupler is cut off, the MOS tube Q1 is cut off, the switch circuit is disconnected, and the corresponding electromagnetic coil is powered off.
When the shaking device works, the MCU alternately outputs low-level signals to the switch circuits corresponding to the electromagnetic coils at the two sides, so that the alternate conduction of the switch circuits at the two sides can be controlled, the alternate electrification of the electromagnetic coils at the two sides is controlled, the continuous operation of the nano-scale magnetic particles in the reaction cup is realized, and reactants are fully reacted.
As another embodiment, as shown in fig. 3, the switching circuit of this embodiment includes an optocoupler U2 and an MOS transistor Q2, in this embodiment, a TLP785 optocoupler is used as the optocoupler, and an N communication MOS transistor is used as the MOS transistor Q2.
An anode of a light emitting diode included in the optocoupler TLP785 is connected with the driving signal SIG2, a cathode of the light emitting diode of the optocoupler TLP785 is grounded, a collector of a phototransistor included in the optocoupler TLP785 is connected with a positive electrode of a power supply, an emitter of the phototransistor is connected with a grid electrode of the MOS tube Q2, a source of Q2 is connected with a positive electrode of a 24V power supply, and a drain of the Q2 is connected with the electromagnetic coil.
According to fig. 3, when the driving signal SIG2 output by the MCU is a high level signal, the light emitting diode in the optocoupler is turned on, the phototriode outputs an electrical signal to the gate of the MOS transistor Q2, the MOS transistor Q2 is controlled to be turned on, and the corresponding electromagnetic coil is energized; when the driving signal SIG2 output by the MCU is a low-level signal, the light-emitting diode in the optical coupler is cut off, the MOS tube Q2 is cut off, the switch circuit is disconnected, and the corresponding electromagnetic coil is powered off.
When the shaking device works, the MCU alternately outputs high-level signals to the switch circuits corresponding to the electromagnetic coils at the two sides, so that the alternate conduction of the switch circuits at the two sides can be controlled, the alternate electrification of the electromagnetic coils at the two sides is controlled, the continuous operation of the nano-scale magnetic particles in the reaction cup is realized, and reactants are fully reacted.
In a further embodiment, a heat dissipation fan is further provided in this embodiment, because a certain amount of heat is generated during the operation of the electromagnetic coil, the temperature of the reaction device during the chemical reaction needs to be kept within a set temperature range (e.g., 37 ± 0.5 ℃), and when the reaction temperature of the reaction device exceeds the set temperature range, the temperature of the reaction device needs to be reduced by using the heat dissipation fan.
Example 2
The embodiment provides a reaction device of a chemiluminescence analyzer, which comprises a reaction cup and a magnetic shaking device, wherein the magnetic shaking device is the same as the reaction device in the embodiment 1 and is not repeated.
Example 3:
this embodiment provides a chemiluminescence analyzer, which includes a reaction apparatus, and the reaction apparatus is the same as the reaction apparatus described in embodiment 2, and is not described again.
In light of the foregoing description of the preferred embodiments according to the present application, it is to be understood that various changes and modifications may be made without departing from the spirit and scope of the invention. The technical scope of the present application is not limited to the contents of the specification, and must be determined according to the scope of the claims.
Claims (8)
1. A magnetic shaking device for a reaction device of a chemiluminescence analyzer is characterized by comprising a group of electromagnetic coils and a control circuit for controlling the on-off of each electromagnetic coil, wherein the group of electromagnetic coils are respectively arranged on two sides of each reaction cup in the reaction device of the chemiluminescence analyzer;
the control circuit comprises a microcontroller and switch circuits connected in series in the energizing loops of the electromagnetic coils, and the microcontroller is used for controlling the alternate on-off of the switch circuits corresponding to the electromagnetic coils on the two sides of the reaction cup.
2. The magnetic shaking apparatus for a chemiluminescent analyzer reaction unit of claim 1 wherein the center of each electromagnetic coil is directly opposite to the nanoscale magnetic particle accumulation zone in the reaction cup.
3. The magnetic shaking-up device for the reaction device of the chemiluminescence analyzer according to claim 1, wherein the switch circuit comprises an optical coupler and an MOS circuit, the MOS circuit comprises an MOS tube, the electrical signal output end of the optical coupler is connected with the control end of the MOS tube, and the electrical input end of the optical coupler is connected with the signal output end of the microcontroller.
4. The magnetic shaking apparatus for a chemiluminescence analyzer reaction apparatus according to claim 3, wherein one switch port of the MOS tube is grounded, and the other switch port is connected with the electromagnetic coil; the optocoupler comprises a light emitting diode, the anode of the light emitting diode is connected with the anode of the power supply, and the cathode of the light emitting diode is connected with the signal output end of the microcontroller.
5. The magnetic shaking apparatus for a chemiluminescent analyzer reaction unit according to claim 3 wherein the optical coupler comprises a light emitting diode, the anode of the light emitting diode is connected to the signal output terminal of the microcontroller and the cathode of the light emitting diode is grounded.
6. The magnetic shaking apparatus for a chemiluminescent analyzer reaction unit according to any one of claims 1 to 5 further comprising a heat sink for cooling the chemiluminescent analyzer reaction unit.
7. A chemiluminescent analyzer reaction device comprising the magnetic shaking device of claim 6.
8. A chemiluminescent analyzer comprising the reaction device of claim 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911072866.2A CN110823875A (en) | 2019-11-05 | 2019-11-05 | Chemiluminescence analyzer and reaction device thereof, and magnetic shaking device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911072866.2A CN110823875A (en) | 2019-11-05 | 2019-11-05 | Chemiluminescence analyzer and reaction device thereof, and magnetic shaking device |
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| Publication Number | Publication Date |
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| CN110823875A true CN110823875A (en) | 2020-02-21 |
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| CN201911072866.2A Pending CN110823875A (en) | 2019-11-05 | 2019-11-05 | Chemiluminescence analyzer and reaction device thereof, and magnetic shaking device |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5466574A (en) * | 1991-03-25 | 1995-11-14 | Immunivest Corporation | Apparatus and methods for magnetic separation featuring external magnetic means |
| CN1779430A (en) * | 2004-11-23 | 2006-05-31 | 三星电子株式会社 | Microfluidic device, and methods of mixing sample and lysing cells using it |
| CN102847477A (en) * | 2012-09-25 | 2013-01-02 | 中国科学院电工研究所 | Magnetic stirring device and stirring method thereof |
| CN106111329A (en) * | 2016-08-16 | 2016-11-16 | 闫维新 | A kind of actuation means of miniature magnetic bead |
| CN207352398U (en) * | 2017-09-13 | 2018-05-11 | 深圳市雷赛智能控制股份有限公司 | General digital input and output multiplex circuit and input-output control unit |
-
2019
- 2019-11-05 CN CN201911072866.2A patent/CN110823875A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5466574A (en) * | 1991-03-25 | 1995-11-14 | Immunivest Corporation | Apparatus and methods for magnetic separation featuring external magnetic means |
| CN1779430A (en) * | 2004-11-23 | 2006-05-31 | 三星电子株式会社 | Microfluidic device, and methods of mixing sample and lysing cells using it |
| CN102847477A (en) * | 2012-09-25 | 2013-01-02 | 中国科学院电工研究所 | Magnetic stirring device and stirring method thereof |
| CN106111329A (en) * | 2016-08-16 | 2016-11-16 | 闫维新 | A kind of actuation means of miniature magnetic bead |
| CN207352398U (en) * | 2017-09-13 | 2018-05-11 | 深圳市雷赛智能控制股份有限公司 | General digital input and output multiplex circuit and input-output control unit |
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Application publication date: 20200221 |