CN112444438B - Sample detection equipment and mixing device thereof - Google Patents
Sample detection equipment and mixing device thereof Download PDFInfo
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- CN112444438B CN112444438B CN201910795331.1A CN201910795331A CN112444438B CN 112444438 B CN112444438 B CN 112444438B CN 201910795331 A CN201910795331 A CN 201910795331A CN 112444438 B CN112444438 B CN 112444438B
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- 238000002156 mixing Methods 0.000 title claims abstract description 78
- 238000001514 detection method Methods 0.000 title abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 118
- 230000000712 assembly Effects 0.000 claims abstract description 115
- 238000000429 assembly Methods 0.000 claims abstract description 115
- 230000005291 magnetic effect Effects 0.000 claims abstract description 95
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 11
- 238000012360 testing method Methods 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 157
- 238000000034 method Methods 0.000 description 11
- 239000008280 blood Substances 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 239000012488 sample solution Substances 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 239000011324 bead Substances 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 239000004065 semiconductor Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 239000012212 insulator Substances 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- -1 oxide Substances 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
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- Chemical & Material Sciences (AREA)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The application discloses sample detection equipment and a mixing device thereof. The mixing device comprises: the device comprises a sample container, a plurality of electromagnetic assemblies and a control circuit, wherein the sample container is used for accommodating a sample liquid, and the sample liquid at least comprises a magnetic carrier; the plurality of electromagnetic assemblies are arranged outside the sample container; the control circuit is connected with the electromagnetic assemblies and is used for controlling the electromagnetic assemblies to be turned on in turn according to a set time sequence so as to generate magnetic force on the magnetic carrier and further mix the magnetic carrier uniformly. Through the mode, the mixing efficiency can be improved.
Description
Technical Field
The application relates to the technical field of medical equipment, in particular to sample detection equipment and a mixing device thereof.
Background
The sample detection device needs to perform a mixing operation in the process of performing sample analysis.
The mixing mode of the stirring and mixing device for the sample detection equipment in the prior art is mainly divided into contact type mixing and non-contact type mixing.
The contact type mixing is mainly carried out by stirring the stirring rod, and liquid can be splashed in the stirring and mixing process, or part of liquid is brought out of the liquid containing device by the stirring rod to cause pollution, and the stirring rod needs to be cleaned again to influence the measurement time of the whole process. If ultrasonic air bubbles are used for mixing uniformly, the generated air bubbles are difficult to remove, and the manufacturing cost is high.
Disclosure of Invention
The application provides sample detection equipment and a mixing device thereof, which are used for solving the problems of lower mixing efficiency and higher cost of the mixing device in the related technology.
In order to solve the technical problems, the application adopts a technical scheme that: a mixing device is provided. The mixing device comprises a sample container, a plurality of electromagnetic assemblies and a control circuit, wherein the sample container is used for accommodating sample liquid, and the sample liquid at least comprises a magnetic carrier; the plurality of electromagnetic assemblies are arranged outside the sample container; the control circuit is connected with the electromagnetic assemblies and is used for controlling the electromagnetic assemblies to be turned on in turn according to a set time sequence so as to generate magnetic force on the magnetic carrier and further mix the magnetic carrier uniformly.
In order to solve the technical problems, the application adopts a technical scheme that: a sample testing device is provided. The sample detection device comprises a mixing device as described above.
Compared with the prior art, the mixing device provided by the application has the advantages that the plurality of electromagnetic assemblies are arranged on the outer side of the sample container, the control circuit is connected with the plurality of electromagnetic assemblies and controls the plurality of electromagnetic assemblies to be turned on in turn according to the set time sequence, namely, the control circuit controls the plurality of electromagnetic assemblies to be electrified in turn according to a certain time sequence to generate a magnetic field, the magnetic field transmits magnetic force generated by the electromagnetic assemblies on the magnetic carrier in the sample container, so that the magnetic carrier moves towards the electrified electromagnetic assemblies, and when the electromagnetic assemblies arranged in different directions are electrified in turn, the magnetic carrier moves towards the electrified electromagnetic assemblies in different directions, so that the magnetic carrier and the sample solution are fully mixed, and the mixing efficiency can be improved.
In addition, as the mixing device provided by the embodiment is only provided with the plurality of electromagnetic assemblies outside the sample container and is used for controlling the plurality of electromagnetic assemblies to be turned on in turn according to the set time sequence by the control circuit, so that the magnetic carrier and the sample solution can be fully mixed, on one hand, the non-contact mixing can be realized, the risk of sample pollution is reduced, the operation of cleaning contact parts is avoided, and the miniaturization design of equipment is facilitated; on the other hand, the electromagnetic assembly is relatively low in price, and the control method is simple and easy to realize, so that the cost of the mixing device can be reduced.
Drawings
For a clearer description of embodiments of the application or of solutions in the prior art, the drawings that are necessary for the description of the embodiments or of the prior art will be briefly described, it being apparent that the drawings in the description below are only some embodiments of the application, from which, without the inventive effort, other drawings can be obtained for a person skilled in the art, in which:
fig. 1 is a first front view schematically showing an embodiment of a blending apparatus according to the present application;
FIG. 2 is a schematic top view of an embodiment of the blending assembly shown in FIG. 1;
fig. 3 is a second front view schematically showing an embodiment of the mixing device of the present application;
fig. 4 is a schematic structural diagram of a control circuit of an embodiment of the blending device of the present application;
fig. 5 is a first elevational schematic of another embodiment of the blending assembly of the present application;
fig. 6 is a schematic top view of another embodiment of the mixing device shown in fig. 5;
fig. 7 is a second elevational schematic view of another embodiment of the blending assembly of the present application;
fig. 8 is a first elevational schematic view of a mixing device according to another embodiment of the application;
FIG. 9 is a schematic top view of a further embodiment of the blending assembly shown in FIG. 8;
fig. 10 is a second elevational view schematically showing a blending apparatus according to still another embodiment of the present application.
Detailed Description
The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
The terms "first", "second" in embodiments of the application are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. Furthermore, the terms "comprise" and "have," as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those listed steps or elements but may include other steps or elements not listed or inherent to such process, method, article, or apparatus.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.
Referring to fig. 1-2 in combination, fig. 1 is a first front view schematically illustrating a blending apparatus according to an embodiment of the present application. Fig. 2 is a schematic top view of an embodiment of the mixing device shown in fig. 1.
In this embodiment, the mixing device 100 includes: a sample container 10, a plurality of electromagnetic assemblies 20, and a control circuit 30.
The sample container 10 is used for accommodating a sample liquid, and the sample liquid at least comprises a magnetic carrier z.
Alternatively, the material of the magnetic carrier z may be a permanent magnetic material. For example, the magnetic carrier z may be magnetic beads, which are spherical. In other embodiments, the magnetic carrier z may have other shapes, which are not limited in this embodiment of the present application. The magnetic carrier z has paramagnetic property and can be rapidly aggregated in a magnetic field.
A plurality of electromagnetic assemblies 20 are disposed outside the sample container 10.
In this embodiment, "plurality" means at least two, for example, two, three, five, or the like.
The plurality of electromagnetic assemblies 20 are disposed at different positions outside the sample container 10, and the plurality of electromagnetic assemblies 20 may be in contact with the outer side wall of the sample container 10 or may be spaced apart from the outer side wall of the sample container 10.
The control circuit 30 is connected with the electromagnetic assemblies 20, and the control circuit 30 is used for controlling the electromagnetic assemblies 20 to be turned on in turn according to a set time sequence so as to generate magnetic force on the magnetic carrier z and further mix the magnetic carrier z uniformly.
The electromagnetic assembly 20 is a device capable of generating electromagnetic force after being electrified, the electromagnetic assembly 20 can be an electromagnet, the electromagnet comprises an iron core and a conductive coil wound outside the iron core, the iron core is magnetized to be a magnet after the conductive coil is electrified, and therefore magnetic force is generated on the magnetic carrier z, and the magnetic carrier z can be uniformly mixed with the magnetic carrier z. The shape of the core may be cylindrical, rectangular parallelepiped, horseshoe or other shape.
Each electromagnetic assembly 20 may be provided with an interface for connection with the control circuit 30.
In this embodiment, the control circuit 30 is connected to the plurality of electromagnetic assemblies 20 and controls the plurality of electromagnetic assemblies 20 to be turned on in turn according to a set time sequence, that is, the control circuit 30 controls the plurality of electromagnetic assemblies 20 to be turned on in turn according to a certain time sequence to generate a magnetic field, the magnetic field transmits magnetic force generated by the electromagnetic assemblies 20 to the magnetic carrier z in the sample container 10, so that the magnetic carrier z moves towards the electrified electromagnetic assemblies 20, and when the electromagnetic assemblies 20 arranged in different directions are turned on in turn, the magnetic carrier z also moves towards the electrified electromagnetic assemblies 20 in different directions, thereby realizing sufficient mixing of the magnetic carrier z and the sample solution and improving mixing efficiency.
In addition, since the mixing device 100 provided in this embodiment only sets the plurality of electromagnetic assemblies 20 on the outer side of the sample container 10, and is used by the control circuit 30 to control the plurality of electromagnetic assemblies 20 to be turned on in turn according to a set time sequence, so that sufficient mixing of the magnetic carrier z and the sample solution can be achieved, on one hand, contactless mixing can be achieved, the risk of sample pollution is reduced, the operation of cleaning contact parts is avoided, and the miniaturized design of the device is facilitated; on the other hand, since the electromagnetic assembly 20 is relatively inexpensive and the control method is simple and easy to implement, the cost of the mixing apparatus 100 can be reduced.
In an application scenario, the sample container 10 may be a test tube, the sample liquid may be a blood sample, the magnetic carrier z is a magnetic bead, the sample container 10 accommodates the blood sample, the blood sample has biological magnetic beads, the magnetic bead may be used for extracting nucleic acid in the blood sample, the control circuit 30 alternately controls the plurality of electromagnetic assemblies 20 to be turned on according to a set time sequence, so as to generate magnetic force on the magnetic ball, so that the magnetic ball is fully and uniformly mixed in the blood sample, and the nucleic acid extraction efficiency is improved.
Referring to fig. 1-3 in combination, fig. 3 is a second front view of an embodiment of a blending device according to the present application.
In the present embodiment, the number of the plurality of electromagnetic assemblies 20 is two, including the electromagnetic assembly a and the electromagnetic assembly B.
Alternatively, as shown in fig. 1, the electromagnetic assembly a and the electromagnetic assembly B may be disposed on the same plane parallel to the liquid surface L of the sample liquid.
Alternatively, as shown in fig. 3, the electromagnetic assembly a and the electromagnetic assembly B may be disposed on different planes parallel to the liquid surface L of the sample liquid.
The liquid level L of the sample liquid may be the highest liquid level L allowed when the sample container 10 accommodates the sample liquid.
Optionally, electromagnetic assembly a and electromagnetic assembly B are disposed opposite outside of sample container 10.
When the electromagnetic assemblies a and B are disposed on the same plane parallel to the liquid surface L of the sample liquid, the relative disposition of the electromagnetic assemblies a and B outside the sample container 10 means that: electromagnetic assemblies a and B each have an angle of 180 ° with respect to the line connecting the centers of the sample containers 10. For example, when the cross-section of the sample container 10 parallel to the liquid surface L is circular in shape, both the electromagnetic assembly a and the electromagnetic assembly B are located on an extension of the diameter of the cross-section.
When the electromagnetic assemblies a and B are disposed on different planes parallel to the liquid surface L of the sample liquid, the relative disposition of the electromagnetic assemblies a and B outside the sample container 10 means that: the line connecting each of the electromagnetic assemblies a and B with the center of the sample container 10 forms an angle of 180 ° between orthographic projections on the same plane parallel to the liquid surface L.
The center of the sample container 10 may refer to a certain center point of a cross section of the sample container 10 parallel to the liquid level L, or may refer to a center line of the sample container 10, and the line connecting the center of the electromagnetic assembly 20 and the center of the sample container 10 may refer to a perpendicular line connecting the center of the electromagnetic assembly 20 and the center line of the sample container 10.
When the electromagnetic components a and B are disposed on different planes parallel to the liquid surface L of the sample liquid, one of the electromagnetic components a and B is disposed at a position near the liquid surface L of the sample liquid, and the other of the electromagnetic components a and B is disposed at a position near the bottom of the sample container 10.
The position close to the liquid level L of the sample liquid means that the distance between the electromagnetic component A or the electromagnetic component B and the liquid level L of the sample liquid is less than half of the liquid column height of the sample liquid. By a position near the bottom of the sample container 10 is meant that the distance of the electromagnetic assembly a or the electromagnetic assembly B from the bottom of the sample container 10 is less than half the liquid column height of the sample liquid.
When one of the electromagnetic component a and the electromagnetic component B is disposed at a position close to the liquid level L of the sample liquid, the other of the electromagnetic component a and the electromagnetic component B is disposed at a position close to the bottom of the sample container 10, and the electromagnetic component a and the electromagnetic component B are disposed outside the sample container 10 relatively, the control circuit 30 alternately controls the electromagnetic component a and the electromagnetic component B to be turned on according to a set time sequence, so that the magnetic carrier z can move in the sample container 10 for a distance as long as possible under the action of magnetic force, thereby fully and uniformly mixing the magnetic carrier z and the sample liquid, reducing the frequency of turning on and off the electromagnetic component a and the electromagnetic component B, improving the mixing efficiency, and prolonging the service life of the device.
Referring to fig. 4 in combination, fig. 4 is a schematic structural diagram of a control circuit 30 of an embodiment of the blending device according to the present application.
Optionally, the control circuit 30 includes: a power supply 31, a plurality of switch assemblies 32, a controller 33.
The plurality of switch assemblies 32 are in one-to-one correspondence with the plurality of electromagnetic assemblies 20, and each switch assembly 32 is connected to the power supply 31 and the corresponding electromagnetic assembly 20.
The plurality of switch assemblies 32 are in one-to-one correspondence with the plurality of electromagnetic assemblies 20, that is, the number of switch assemblies 32 and the number of electromagnetic assemblies 20 are equal, and one switch assembly 32 is correspondingly connected with one electromagnetic assembly 20.
The controller 33 is connected to the plurality of switch assemblies 32, and the controller 33 is configured to alternately control the on/off of the plurality of switch assemblies 32 according to a set time sequence, wherein the power source 31 provides power to the corresponding electromagnetic assembly 20 when the switch assemblies 32 are turned on.
The power supply 31 supplies power to the corresponding electromagnetic assembly 20, and the electromagnetic assembly 20 generates a magnetic field after being electrified, and the magnetic field transmits the magnetic force generated by the electromagnetic assembly 20 to the magnetic carrier z in the sample container 10.
Alternatively, the controller 33 may be an integrated circuit chip having signal processing capabilities. The controller 33 may also be a Microprocessor (MCU), a Digital Signal Processor (DSP), an Application Specific Integrated Circuit (ASIC), an off-the-shelf programmable gate array (FPGA) or other programmable logic device, discrete gate or transistor logic device, discrete hardware components.
Optionally, referring to fig. 4, the switch component 32 is a MOS transistor, a source of the MOS transistor is connected to the power source 31, a drain of the MOS transistor is connected to the corresponding electromagnetic component 20, and a gate of the MOS transistor is connected to the controller 33.
MOS transistors are metal, oxide, semiconductor field effect transistors, or metal-insulator, semiconductors. The MOS tube has the advantages of small volume and electricity saving, is beneficial to the miniaturization design of the device and reduces the running cost of the device.
Referring to fig. 5, 6 and 7, fig. 5 is a schematic front view of a mixing device according to another embodiment of the application. Fig. 6 is a schematic top view of another embodiment of the mixing device shown in fig. 5. Fig. 7 is a second elevational schematic view of another embodiment of the blending assembly of the present application.
Alternatively, the number of electromagnetic assemblies 20 is three, including electromagnetic assemblies C and D and electromagnetic assembly E.
Please refer to fig. 6. The angle between the line connecting each two adjacent electromagnetic assemblies 20 and the center of the sample container 10 is 120 °.
Alternatively, as shown in fig. 5, the electromagnetic assemblies C and D and the electromagnetic assembly E (which are shielded by the sample container in the drawing) may be disposed on the same plane parallel to the liquid surface L of the sample liquid.
Alternatively, as shown in fig. 7, the electromagnetic assemblies C and D and the electromagnetic assembly E (which are shielded by the sample container in the drawing) may be disposed on different planes parallel to the liquid surface L of the sample liquid.
For example, the electromagnetic assemblies C and E are disposed on a first plane parallel to the liquid surface L of the sample liquid, and the electromagnetic assembly D is disposed on a second plane parallel to the liquid surface L of the sample liquid, the first plane and the second plane being non-coplanar.
Further, the electromagnetic component D may be disposed at a position near the liquid level L of the sample liquid, the electromagnetic component C and the electromagnetic component E may be disposed at a position near the bottom of the sample container 10, and the control circuit 30 alternately controls the electromagnetic component C, the electromagnetic component D, and the electromagnetic component E to be turned on according to a set time sequence, so that the magnetic carrier z may move as long as possible in the sample container 10 under the action of magnetic force, thereby fully mixing the magnetic carrier z and the sample liquid, reducing the frequency of turning on and off the electromagnetic component C, the electromagnetic component D, and the electromagnetic component E, improving the mixing efficiency, and prolonging the service life of the device.
The position close to the liquid level L of the sample liquid means that the distance between the electromagnetic assembly D and the liquid level L of the sample liquid is less than half of the liquid column height of the sample liquid. By a position near the bottom of the sample container 10 is meant that the distance of the electromagnetic assemblies C and E from the bottom of the sample container 10 is less than half the liquid column height of the sample liquid.
In other embodiments, the electromagnetic assemblies C and D and the electromagnetic assembly E may be disposed on three different planes parallel to the liquid surface L of the sample liquid, respectively.
When the electromagnetic assemblies C and D and the electromagnetic assembly E may also be disposed on different planes parallel to the liquid surface L of the sample liquid, an angle of 120 ° between the line connecting the two adjacent electromagnetic assemblies 20 and the center of the sample container 10 means that: the electromagnetic assemblies C and D and the electromagnetic assemblies 20 are equidistantly arranged around the outer side of the sample container 10.
When the electromagnetic assemblies C and D and the electromagnetic assembly E may also be disposed on different planes parallel to the liquid surface L of the sample liquid, an angle of 120 ° between the line connecting the two adjacent electromagnetic assemblies 20 and the center of the sample container 10 means that: in the electromagnetic assemblies C and D and the electromagnetic assembly E, the included angle between the orthographic projections of each two adjacent electromagnetic assemblies 20, each of which is connected to the center of the sample container 10, on the same plane parallel to the liquid surface L is 120 °.
The center of the sample container 10 may refer to a certain center point of a cross section of the sample container 10 parallel to the liquid level L, or may refer to a center line of the sample container 10, and the line connecting the center of the electromagnetic assembly 20 and the center of the sample container 10 may refer to a perpendicular line connecting the center of the electromagnetic assembly 20 and the center line of the sample container 10.
Referring to fig. 8, 9 and 10, fig. 8 is a schematic front view of a blending apparatus 100 according to another embodiment of the present application. Fig. 9 is a schematic top view of another embodiment of the mixing device 100 shown in fig. 8. Fig. 10 is a second front view of a mixing device 100 according to another embodiment of the present application.
In the present embodiment, the number of the plurality of electromagnetic assemblies 20 is four.
Please refer to fig. 9. Every two adjacent electromagnetic assemblies 20 are at an angle of 90 ° to the line connecting the centers of the sample containers 10.
Alternatively, as shown in fig. 8, four and electromagnetic assemblies 20 may each be disposed on the same plane parallel to the liquid surface L of the sample liquid. Then a 90 ° angle between the line connecting each two adjacent electromagnetic assemblies 20 to the center of the sample container 10 means that: four electromagnetic assemblies 20 are equidistantly arranged around the sample container 10.
Alternatively, as shown in fig. 10, four electromagnetic assemblies 20 may be disposed on different planes parallel to the liquid surface L of the sample liquid. Then a 90 ° angle between the line connecting each two adjacent electromagnetic assemblies 20 to the center of the sample container 10 means that: of the four electromagnetic assemblies 20, each two adjacent electromagnetic assemblies 20 each have a 90 ° angle with respect to the orthographic projection of the same plane parallel to the liquid surface L, in the line connecting the centers of the sample containers 10.
The center of the sample container 10 may refer to a certain center point of a cross section of the sample container 10 parallel to the liquid level L, or may refer to a center line of the sample container 10, and the line connecting the center of the electromagnetic assembly 20 and the center of the sample container 10 may refer to a perpendicular line connecting the center of the electromagnetic assembly 20 and the center line of the sample container 10.
Optionally, with continued reference to fig. 10, the plurality of electromagnetic assemblies 20 includes a first electromagnetic assembly 21, a second electromagnetic assembly 22, a third electromagnetic assembly 23, and a fourth electromagnetic assembly 24 disposed adjacent one another in sequence.
Wherein the first and third electromagnetic assemblies 21, 23 are disposed on a first plane parallel to the liquid level L of the sample liquid, and the second and fourth electromagnetic assemblies 22, 24 (obscured by the sample container) are disposed on a second plane parallel to the liquid level L of the sample liquid, the first and second planes not being coplanar.
The plurality of electromagnetic assemblies 20 including sequentially adjacent arrangement means: the first electromagnetic assembly 21, the second electromagnetic assembly 22, the third electromagnetic assembly 23, and the fourth electromagnetic assembly 24 are disposed adjacent to each other in order outside the sample container 10 in the circumferential direction of the sample container 10.
Optionally, the first plane is near the liquid level L of the sample liquid and the second plane is near the bottom of the sample container 10.
Wherein, the liquid level L of the first plane near the sample liquid means: the vertical distance between the first plane and the liquid level L of the sample liquid is less than half the liquid column height of the sample liquid. The second plane near the bottom of the sample container 10 means: the second plane is perpendicular to the bottom of the sample container 10 less than half the height of the liquid column of the sample liquid.
Alternatively, the control circuit 30 alternately controls the first electromagnetic assembly 21, the second electromagnetic assembly 22, the third electromagnetic assembly 23, and the fourth electromagnetic assembly 24 to be turned on according to a set timing.
Because the first electromagnetic component 21 and the third electromagnetic component 23 are arranged on the first plane parallel to the liquid level L of the sample liquid, the second electromagnetic component 22 and the fourth electromagnetic component 24 are arranged on the second plane parallel to the liquid level L of the sample liquid, the first plane is close to the liquid level L of the sample liquid, the second plane is close to the bottom of the sample container 10, and the control circuit 30 alternately controls the first electromagnetic component 21, the second electromagnetic component 22, the third electromagnetic component 23 and the fourth electromagnetic component 24 to be started according to a set time sequence, so that the magnetic carrier z moves in the sample container 10 for a distance as long as possible under the action of magnetic force, thereby fully mixing the magnetic carrier z and the sample liquid, improving the mixing efficiency and prolonging the service life of the device.
Wherein the on-time of the first electromagnetic assembly 21 and the third electromagnetic assembly 23 is greater than the on-time of the second electromagnetic assembly 22 and the fourth electromagnetic assembly 24.
In the vertical direction, since the liquid level L of the sample liquid is necessarily located above the bottom of the sample container 10, the first electromagnetic assembly 21 and the third electromagnetic assembly 23 located on the first plane are also necessarily located above the second electromagnetic assembly 22 and the fourth electromagnetic assembly 24 located on the second plane, when the magnetic carrier z is adsorbed and moved from the position of the lower liquid level L to the position of the higher liquid level L, the resistance force is greater than the resistance force of the magnetic carrier z, and the movement speed is slower, so that the opening time of the first electromagnetic assembly 21 and the third electromagnetic assembly 23 is set to be greater than the opening time of the second electromagnetic assembly 22 and the fourth electromagnetic assembly 24, so that the magnetic carrier z can be ensured to be adsorbed to the preset position, the magnetic carrier z and the sample liquid can be fully mixed, and the mixing efficiency can be improved.
Alternatively, the control circuit 30 alternately controls the first electromagnetic assembly 21, the second electromagnetic assembly 22, the third electromagnetic assembly 23 and the fourth electromagnetic assembly 24 to be turned on according to a set time sequence, wherein the magnetic force provided by the first electromagnetic assembly 21 and the third electromagnetic assembly 23 when turned on is greater than the magnetic force provided by the second electromagnetic assembly 22 and the fourth electromagnetic assembly 24 when turned on.
To make the magnetic force provided by the first electromagnetic assembly 21 and the third electromagnetic assembly 23 when turned on greater than the magnetic force provided by the second electromagnetic assembly 22 and the fourth electromagnetic assembly 24 when turned on, for example, the current when the first electromagnetic assembly 21 and the third electromagnetic assembly 23 are turned on may be controlled by the control circuit 30 to be greater than the current when the second electromagnetic assembly 22 and the fourth electromagnetic assembly 24 are turned on.
Alternatively, the electromagnetic assembly 20 may be an electromagnet. To make the magnetic force provided at the time of opening the first and third electromagnetic assemblies 21 and 23 larger than the magnetic force provided at the time of opening the second and fourth electromagnetic assemblies 22 and 24, for example, the number of turns of the coils wound on the iron core by setting the first and third electromagnetic assemblies 21 and 23 larger than the number of turns of the coils wound on the iron core by setting the first and fourth electromagnetic assemblies 22 and 24 or the distance between the coils wound on the iron core by setting the first and third electromagnetic assemblies 21 and 23 smaller than the distance between the coils wound on the iron core by setting the second and fourth electromagnetic assemblies 22 and 24.
Since the liquid level L of the sample liquid is necessarily located above the bottom of the sample container 10 in the vertical direction, the first and third electromagnetic assemblies 21 and 23 located on the first plane are also necessarily located above the second and fourth electromagnetic assemblies 22 and 24 located on the second plane, and when the magnetic carrier z is adsorbed and moved from the position of the lower liquid level L to the position of the higher liquid level L, the resistance force is greater than the resistance force of the magnetic carrier z to the position of the lower liquid level L by the position of the higher liquid level L, so that the magnetic force provided when the first and third electromagnetic assemblies 21 and 23 are turned on is greater than the magnetic force provided when the second and fourth electromagnetic assemblies 22 and 24 are turned on, the magnetic carrier z can be ensured to be adsorbed to the preset position, and the moving time can be shortened, and the mixing efficiency can be further improved while achieving sufficient mixing of the magnetic carrier z and the sample liquid.
The sample detection apparatus according to the embodiment of the present application includes the mixing device 100 described in the above embodiment.
Alternatively, the sample detection apparatus may include a plurality of mixing devices 100, such as two, three, five or ten.
Optionally, the sample detection device may further comprise a sample introduction device, a sample addition device, a conveying device, a sampling detection device, a reaction device, an incubation device, a magnetic separation device, and the like. For example, before the sample is sent to the sampling and detecting device for sampling and detection, whether the sample is mixed by the mixing device 100 can be determined according to the property of the sample, for example, when the sample is detected by a suspension such as a blood sample, the sample can be mixed by the mixing device 100.
In one application example, the mixing device 100 in this embodiment may be combined with an incubation device, for example, a plurality of electromagnetic assemblies 20 in the mixing device 100 are disposed in a base of the incubation device, and a control circuit 30 is connected to the plurality of electromagnetic assemblies 20, and the control circuit 30 is used for controlling the plurality of electromagnetic assemblies 20 to be turned on in turn according to a set time sequence so as to generate magnetic force on the magnetic carrier z, so that the sample liquid and the magnetic carrier z are fully mixed while the sample liquid is incubated.
In another application example, the sample detection apparatus may add the sample liquid and the magnetic carrier z to the sample container 10 of the mixing device 100 by the sample adding device, and sufficiently mix the sample liquid and the magnetic carrier z by the mixing device 100.
According to the mixing device provided by the application, the plurality of electromagnetic assemblies are arranged on the outer side of the sample container of the reaction container, the control circuit is connected with the plurality of electromagnetic assemblies and controls the plurality of electromagnetic assemblies to be turned on in turn according to the set time sequence, namely, the control circuit controls the plurality of electromagnetic assemblies to be electrified in turn according to a certain time sequence to generate a magnetic field, the magnetic field transmits magnetic force generated by the electromagnetic assemblies on the magnetic carrier in the sample container, so that the magnetic carrier moves towards the electrified electromagnetic assemblies, and when the electromagnetic assemblies arranged in different directions are electrified in turn, the magnetic carrier moves towards the electrified electromagnetic assemblies in different directions, so that the magnetic carrier and the sample solution are fully mixed, and the mixing efficiency can be improved.
In addition, as the mixing device provided by the embodiment is only provided with the plurality of electromagnetic assemblies outside the sample container and is used for controlling the plurality of electromagnetic assemblies to be turned on in turn according to the set time sequence by the control circuit, so that the magnetic carrier and the sample solution can be fully mixed, on one hand, the non-contact mixing can be realized, the risk of sample pollution is reduced, the operation of cleaning contact parts is avoided, and the miniaturization design of equipment is facilitated; on the other hand, the electromagnetic assembly is relatively low in price, and the control method is simple and easy to realize, so that the cost of the mixing device can be reduced.
The foregoing description is only illustrative of the present application and is not intended to limit the scope of the application, and all equivalent structures or equivalent processes or direct or indirect application in other related technical fields are included in the scope of the present application.
Claims (4)
1. A blending device, characterized in that it comprises:
the sample container is used for accommodating a sample liquid, and the sample liquid at least comprises a magnetic carrier;
a plurality of electromagnetic assemblies disposed outside the sample container; the plurality of electromagnetic assemblies comprise a first electromagnetic assembly, a second electromagnetic assembly, a third electromagnetic assembly and a fourth electromagnetic assembly which are sequentially and adjacently arranged on the outer side of the sample container along the circumferential direction of the sample container, and an included angle between a connecting line of every two adjacent electromagnetic assemblies and the center of the sample container is 90 degrees; the first electromagnetic assembly and the third electromagnetic assembly are arranged on a first plane parallel to the liquid level of the sample liquid, the second electromagnetic assembly and the fourth electromagnetic assembly are arranged on a second plane parallel to the liquid level of the sample liquid, the first plane and the second plane are not coplanar, the first plane is close to the liquid level of the sample liquid, and the second plane is close to the bottom of the sample container;
the control circuit is used for controlling the first electromagnetic assembly, the second electromagnetic assembly, the third electromagnetic assembly and the fourth electromagnetic assembly to be turned on in turn according to a set time sequence, wherein magnetic force provided by the first electromagnetic assembly and the third electromagnetic assembly when being turned on is larger than magnetic force provided by the second electromagnetic assembly and the fourth electromagnetic assembly when being turned on, and the turn-on time of the first electromagnetic assembly and the third electromagnetic assembly is larger than the turn-on time of the second electromagnetic assembly and the fourth electromagnetic assembly; the control circuit controls the electromagnetic assemblies to be electrified in turn according to a set time sequence to generate a magnetic field, and the magnetic field transmits magnetic force generated by the electromagnetic assemblies on the magnetic carrier in the sample container so that the magnetic carrier moves towards the electrified electromagnetic assemblies;
the number of turns of the coils wound on the iron core of the first electromagnetic assembly and the third electromagnetic assembly is larger than the number of turns of the coils wound on the iron core of the second electromagnetic assembly and the fourth electromagnetic assembly; or, the distance between the coils wound in the first electromagnetic assembly and the third electromagnetic assembly and the iron core is smaller than the distance between the coils wound in the second electromagnetic assembly and the fourth electromagnetic assembly and the iron core.
2. The blending assembly of claim 1, wherein the control circuit comprises:
a power supply;
the switch assemblies are in one-to-one correspondence with the electromagnetic assemblies, and each switch assembly is connected with the power supply and the corresponding electromagnetic assembly;
and the controller is connected with the switch assemblies and is used for controlling the switch assemblies to be turned on and off in turn according to a set time sequence, wherein when the switch assemblies are turned on, the power supply supplies electric energy to the corresponding electromagnetic assemblies.
3. The mixing device according to claim 2, wherein,
the switch component is an MOS tube, a source electrode of the MOS tube is connected with the power supply, a drain electrode of the MOS tube is connected with the corresponding electromagnetic component, and a grid electrode of the MOS tube is connected with the controller.
4. A sample testing device, characterized in that it comprises a mixing apparatus according to any one of claims 1-3.
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| CN1779430A (en) * | 2004-11-23 | 2006-05-31 | 三星电子株式会社 | Microfluidic device, and methods of mixing sample and lysing cells using it |
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