CN110810849A - Composition for increasing bone mineral density and application thereof - Google Patents
Composition for increasing bone mineral density and application thereof Download PDFInfo
- Publication number
- CN110810849A CN110810849A CN201911154001.0A CN201911154001A CN110810849A CN 110810849 A CN110810849 A CN 110810849A CN 201911154001 A CN201911154001 A CN 201911154001A CN 110810849 A CN110810849 A CN 110810849A
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- China
- Prior art keywords
- powder
- bone density
- increasing
- health food
- increasing bone
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- Pending
Links
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- CSSYQJWUGATIHM-IKGCZBKSSA-N l-phenylalanyl-l-lysyl-l-cysteinyl-l-arginyl-l-arginyl-l-tryptophyl-l-glutaminyl-l-tryptophyl-l-arginyl-l-methionyl-l-lysyl-l-lysyl-l-leucylglycyl-l-alanyl-l-prolyl-l-seryl-l-isoleucyl-l-threonyl-l-cysteinyl-l-valyl-l-arginyl-l-arginyl-l-alanyl-l-phenylal Chemical compound C([C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 CSSYQJWUGATIHM-IKGCZBKSSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
- A23L33/155—Vitamins A or D
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L15/00—Egg products; Preparation or treatment thereof
- A23L15/30—Addition of substances other than those covered by A23L15/20 – A23L15/25
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a composition for increasing bone density, which comprises the following raw materials: milk mineral salt, hydrolyzed yolk powder, and vitamin D3Micro-capsule powder and whole milk powder. The invention also discloses application of the composition for increasing the bone density in preparing food and health-care food for increasing the bone density. The composition for increasing the bone density provided by the invention is novel in component matching, supplements required functional raw materials from each link of bone formation, promotes bone health from each link of bone growth, formation and metabolism, and is different from calcium supplement products for increasing the bone density sold in the market.
Description
Technical Field
The invention belongs to the technical field of health-care food, and particularly relates to a composition for increasing bone mineral density, a functional active ingredient and application thereof.
Background
The health food belongs to one of special foods and is essentially different from common foods. Health foods are foods claimed to have a specific health function or for the purpose of supplementing vitamins and minerals. The food is suitable for specific people to eat, has the function of regulating the organism, does not aim at treating diseases, does not produce any acute, subacute or chronic harm to the human body, and has the claimed function meeting the requirements of 'inspection and evaluation technical specifications of health food', so the functional active ingredients of the formula of the food must meet the requirements of research and development and evaluation of the health food, ensure the body regulating function of the food in the human body, and have dose-effect relationship. The most essential difference from health food is that the common food is prohibited to claim and has the function of regulating the organism, so the amount of the functional active ingredients in the formula is definitely lower than the amount capable of playing the function of regulating the organism in the human body, and meanwhile, the common food does not have any corresponding legal standard of quality and function evaluation. From the specific product development and orientation, the health food and the common food are essentially different.
At present, products for supplementing calcium and the like which are mostly accepted by middle-aged and elderly people contain milk powder as a basic substance. Health foods on the market, which enhance bone density, are focused almost on the following three aspects. On one hand, the relationship of inorganic calcium crystal forms to strengthening bones is mostly studied, and calcium sources which are identical with the proportion of basic substances forming human bones are ignored. On the other hand, the polypeptide is used together to improve calcium absorption, weaken osteoclast function and inhibit bone resorption, but the development of polypeptide varieties is relatively single, and only casein phosphopeptide and hydrolyzed collagen are used temporarily. In yet another aspect, cartilage repair is provided by supplementation with an amino monosaccharide, such as glucosamine, in combination with calcium; supplementation with amino monosaccharides and glycosaminoglycans, such as glucosamine and chondroitin sulfate, in combination, results in analgesia, reversing damaged cartilage, and neglecting concerns over the overall physiological phase of bone.
The invention provides a 'osteoporosis preventing old-aged formula milk powder' named in 26.04.2017.No. CN106578094, which discloses the advantages of the osteoporosis preventing old-aged formula milk powder, can better improve the dietary calcium source and the calcium absorption and utilization rate, enhance the bone density and prevent and delay osteoporosis of old people. The method has the following defects: firstly, although the claims are dependent on 'lactoferrin, hydrolyzed yolk powder, casein phosphopeptide and milk mineral salt', the method ignores the characteristic that vitamin A participates in endochondral osteogenesis in the preparation of a nutrition evaluation sample and is applied to 233.33-533.34 mug/day of middle-aged and elderly people in health food; fructo-oligosaccharide of common food can promote mineral substance absorption, and the batch is 4-30 g/day for adults when the fructo-oligosaccharide is applied to health food; the calcium for maintaining the bone health is applied to health-care food, and the adult amount of the calcium is 200-800 mg/day mostly; the vitamin D for promoting calcium and phosphorus absorption is applied to health food, namely the middle-aged and elderly people, and the dosage is 3.33-6.67 mu g/day (Yangyuexin, plum, and the like, a nutritional functional component application guide [ M ] Beijing university medical publisher: 2011.). The dependent claims of the invention are therefore considered to be equally effective in the prevention of osteoporosis. Secondly, the grouping of experimental animals is not strict, the scientific requirements of the animal grouping of the health food inspection and evaluation technical specification on the bone density increasing function inspection method are not met, the advantages of the main claim in preventing osteoporosis cannot be proved, the inertia of the dependent claims in preventing osteoporosis cannot be proved, and the advantages of the invention in preventing osteoporosis cannot be defined.
Chinese patent No. CN102763729, published as 11/07/2012, entitled composite bovine colostrum powder for improving bone density, discloses that a developed bovine colostrum product is relatively single in components and functions and mostly focuses on immune characteristics in the technical field of health foods, so that a composite bovine colostrum powder is developed to improve immunity, increase bone density and improve osteoporosis. The method has the following defects: firstly, preparing high-quality calcium source and components for promoting calcium absorption and calcium attachment by using chitosan which is used for regulating immunity, regulating body fluid balance and assisting in improving body acid-base balance by using active factors in colostrum to form a group of compound bovine colostrum powder for improving bone density. Its essence depends on calcium acetate, collagen powder and vitamin D3Can be used together for improving osteoporosis. The effect of bovine colostrum on increasing bone density is not clear in this invention.Secondly, vitamin D is used in the formula3Microcapsule powder (50 ten thousand IU/g). For vitamin D only3Vitamin D in microencapsulated powder3The content is limited, and is not less than 50 ten thousand IU/g. In fact, vitamin D3The micro-capsule powder (10 ten thousand IU/g) or (50 ten thousand IU/g) can achieve the purposes of increasing the bone density and improving the osteoporosis. Finally, a pathogenic pathological animal model is obviously established, an osteoporosis-causing test is used, and the effect of the invention on improving the bone density of rats is investigated. The invention does not meet the experimental principle requirement of health food inspection and evaluation technical specification on a function inspection method for increasing bone mineral density, breaks away from the essential characteristic that health food does not aim at treating diseases, and can not prove that the invention belongs to the technical field of health food and is health food for improving bone mineral density.
Therefore, the invention fills the blank of the product development.
Disclosure of Invention
Based on the above, the present invention aims to overcome the disadvantages of the prior art and provide a composition for increasing bone density and a functional active ingredient, which have novel component matching, supplement the required functional raw materials and functional active ingredients from each link of bone formation, promote bone health from each link of bone growth, formation and metabolism, and are different from the bone density increasing products sold in the market.
In order to achieve the purpose, the invention adopts the technical scheme that: a composition for increasing bone density comprising the following raw materials: milk mineral salt, hydrolyzed yolk powder, and vitamin D3Micro-capsule powder and whole milk powder.
Preferably, every 100 preparation units comprise the following raw materials: 4.32-1.00 kg of milk mineral salt, 1.20-275.60 g of hydrolyzed yolk powder and vitamin D30.005-1.16 g of micro-capsule powder and 0.15-383.30 g of whole milk powder.
The functional active ingredients of the composition for increasing bone density comprise calcium, peptide and vitamin D3Every 100 preparation units contain 11.0-20.0 g of calcium, 8.0-22.0 g of peptide and vitamin D3116.0～216.0μg。
The invention also provides application of the composition for increasing the bone density in preparing health-care food for increasing the bone density.
The composition for increasing the bone density can be used for preparing health-care food for increasing the bone density.
The invention also aims to provide a health food for increasing bone density, which comprises the composition for increasing bone density and auxiliary materials acceptable in the field of health foods.
Preferably, the auxiliary materials acceptable in the field of health-care food comprise solid preparation auxiliary materials and/or adhesives.
Preferably, every 100 preparation units of the health food comprises the following raw materials: 4.32-1.00 kg of milk mineral salt, 1.20-275.60 g of hydrolyzed yolk powder and vitamin D30.005 g-1.16 g of micro-capsule powder, 0.15 g-383.30 g of whole milk powder, 1.52g-5.66g of solid preparation auxiliary material and/or 0.02g-2.00g of adhesive.
Preferably, the solid formulation auxiliary material is selected from at least one of soy protein isolate, maltodextrin, corn starch and resistant dextrin; the adhesive is selected from at least one of sodium carboxymethylcellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, starch slurry, purified water, methyl cellulose, ethyl cellulose, hydroxypropyl methyl cellulose, polyvinylpyrrolidone and sorbitol.
Preferably, the health food dosage forms include powders, tablets and capsules.
The invention also provides a preparation method of the health food for increasing the bone mineral density, and raw materials are mixed in an equivalent increasing and multiple amplifying mode.
Preferably, the raw materials are mixed using at least one of a three-dimensional mixer, a double cone mixer, a V-type mixer, a two-dimensional mixer, a spray dryer, a fluidized bed spray dryer, a powder filling and sealing machine, a tablet press, a coating machine, a granulation machine, a hard capsule machine, and a soft capsule machine.
Preferably, the preparation method of the powder health food comprises the following steps:
(1) weighing the prescription amount of milk mineral salt, hydrolyzed yolk powder and vitaminsElement D3Micro-capsule powder, whole milk powder, and/or solid preparation auxiliary materials and/or adhesives;
(2) putting the hydrolyzed yolk powder, the whole milk powder and/or the solid preparation auxiliary material into a three-dimensional mixer, and uniformly mixing to obtain first-grade premixed powder;
(3) adding milk mineral salt into the first-stage premixed powder, placing the mixture into a three-dimensional mixer, performing second-stage mixing, and uniformly mixing to obtain second-stage premixed powder which is named as mixed powder A;
(4) with vitamin D3The total amount of the microcapsule powder is 1, and the materials with the same weight are taken from the mixed powder A and mixed with the mixed powder A for the 1 st time; on the basis, adding the mixed powder A with the same weight for the 2 nd mixing; and so on. And mixing the two powders completely to obtain the total mixed powder.
(5) And (3) placing the total mixed powder into a powder filling and sealing machine, subpackaging, filling and sealing to obtain the powder health-care food.
Preferably, the preparation method of the tablet health food comprises the following steps:
(1) weighing the prescription amount of milk mineral salt, hydrolyzed yolk powder and vitamin D3Microcapsule powder, whole milk powder, solid preparation auxiliary material and/or adhesive;
(2) putting the solid preparation auxiliary materials into a three-dimensional mixer, uniformly mixing, and then adding the hydrolyzed yolk powder and the whole milk powder to obtain first-grade premixed powder;
(3) adding milk mineral salt into the first-stage premixed powder, performing second-stage mixing, and uniformly mixing to obtain second-stage premixed powder which is named as mixed powder A;
(4) with vitamin D3The total amount of the microcapsule powder is 1, and the materials with the same weight are taken from the mixed powder A and mixed with the mixed powder A for the 1 st time; on the basis, adding the mixed powder A with the same weight for the 2 nd mixing; and so on. Mixing the two powders completely to obtain total mixed powder;
(5) taking the total amount of the binder as 1, and taking the materials with equal weight from the total mixed powder to mix with the binder for the 1 st time; on the basis, adding the 2 nd mixing with the total mixed powder with the same weight; and so on. Mixing the two materials completely to obtain powder to be tabletted;
(6) and tabletting the powder to be tabletted to obtain the tablet health-care food.
Preferably, the preparation method of the capsule health food comprises the following steps:
(1) weighing the prescription amount of milk mineral salt, hydrolyzed yolk powder and vitamin D3Microcapsule powder, whole milk powder, solid preparation auxiliary material and/or adhesive;
(2) putting the solid preparation auxiliary materials into a three-dimensional mixer, uniformly mixing, and then adding the hydrolyzed yolk powder and the whole milk powder to obtain first-grade premixed powder;
(3) adding milk mineral salt into the first-stage premixed powder, performing second-stage mixing, and uniformly mixing to obtain second-stage premixed powder which is named as mixed powder A;
(4) with vitamin D3The total amount of the microcapsule powder is 1, and the materials with the same weight are taken from the mixed powder A and mixed with the mixed powder A for the 1 st time; on the basis, adding the mixed powder A with the same weight for the 2 nd mixing; and so on. Mixing the two powders completely to obtain total mixed powder;
(5) taking the total amount of the binder as 1, and taking the materials with equal weight from the total mixed powder to mix with the binder for the 1 st time; on the basis, adding the 2 nd mixing with the total mixed powder with the same weight; and so on. Mixing the two materials completely to obtain powder to be filled;
(6) and (3) putting the powder to be filled into a hard capsule machine, and filling capsules to obtain the capsule health food.
Compared with the prior art, the invention has the beneficial effects that: (1) the composition for increasing the bone mineral density and the functional active ingredients thereof are novel in collocation, supplement the required functional raw materials from each link of bone formation, promote the bone health from each link of bone growth, formation and metabolism, and are different from the calcium supplement products for increasing the bone mineral density sold in the market; (2) in the composition of the present invention, vitamin D3The microcapsule powder has low proportion and is not easy to be uniformly mixed with other materials, and in order to ensure that the microcapsule powder is uniformly mixed in a prescription, a mixing mode of an equivalent progressive multiple amplification mode is adopted; (3) the density of the hydrolyzed yolk powder is higher than that of the rest materialsThe materials have small density and are easy to scatter, the selectable solid preparation form is limited, and the health food of the invention effectively solves the problem by adding the adhesive.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
Example 1
One embodiment of the bone density increasing composition of the present invention comprises the following raw materials per 100 formulation units: milk mineral salt 180.00g, hydrolyzed yolk powder 50.00g, vitamin D30.21g of micro-capsule powder and 69.79g of whole milk powder.
Example 2
One embodiment of the bone density increasing composition of the present invention comprises the following raw materials per 100 formulation units: 300.00g of milk mineral salt, 83.50g of hydrolyzed yolk powder and vitamin D30.35g of microcapsule powder and 93.07g of whole milk powder.
Example 3
One embodiment of the bone density increasing composition of the present invention comprises the following raw materials per 100 formulation units: 15.00g of milk mineral salt, 4.18g of hydrolyzed yolk powder and vitamin D30.018g of micro-capsule powder and 0.15g of whole milk powder.
Example 4
One embodiment of the bone density increasing composition of the present invention comprises the following raw materials per 100 formulation units: milk mineral salt 4.80g, hydrolyzed yolk powder 1.34g, vitamin D30.0056g of microcapsule powder and 0.34g of whole milk powder.
Example 5
One embodiment of the bone density increasing composition of the present invention comprises the following raw materials per 100 formulation units: milk mineral salt 1.00kg, hydrolyzed yolk powder 275.60g, vitamin D31.16g of microcapsule powder and 383.30g of whole milk powder.
Example 6
One embodiment of the bone density increasing composition of the present invention comprises the following raw materials per 100 formulation units: milk mineral salt 4.32g, hydrolyzed yolk powder 1.20g, vitamin D30.005g of microcapsule powder and 0.15g of whole milk powder.
Example 7
One embodiment of the bone density increasing composition of the present invention comprises the following raw materials per 100 formulation units: 502.16g of milk mineral salt, 138.4g of hydrolyzed yolk powder and vitamin D30.5825g of microcapsule powder and 191.73g of whole milk powder.
Example 8
According to one embodiment of the health food for increasing bone density, the health food is powder and comprises the following raw materials: the bone density increasing composition described in example 1.
The preparation method of the powder health-care food comprises the following steps:
(1) weighing 180.00g of milk mineral salt, 50.00g of hydrolyzed yolk powder and vitamin D according to the prescription amount30.21g of micro-capsule powder and 69.79g of whole milk powder;
(2) putting 50.00g of hydrolyzed yolk powder and 69.79g of whole milk powder into a three-dimensional mixer, and uniformly mixing to obtain first-grade premixed powder;
(3) adding 180.00g of milk mineral salt into the primary premixed powder, placing the mixture into a three-dimensional mixer, carrying out secondary mixing, and uniformly mixing to obtain secondary premixed powder which is named as mixed powder A;
(4) with vitamin D30.21 total amount of microcapsule powder is 1, and equal weight of materials are taken from the mixed powder A and mixed with the powder A for the 1 st time; on the basis, adding the mixed powder A with the same weight for the 2 nd mixing; and so on. Mixing the two powders completely to obtain total mixed powder;
(5) and (3) putting the total mixed powder into a powder filling and sealing machine, subpackaging by 3 g/bag, filling and sealing to obtain the powder health-care food.
Example 9
According to one embodiment of the health food for increasing bone density, the health food is powder and comprises the following raw materials: the bone density increasing composition described in example 2 and maltodextrin 23.08 g.
The preparation method of the powder health-care food comprises the following steps:
(1) balanceTaking 300.00g of milk mineral salt, 83.50g of hydrolyzed yolk powder and vitamin D according to the prescription amount30.35g of microcapsule powder, 93.07g of whole milk powder and 23.08g of maltodextrin;
(2) placing 83.50g of hydrolyzed yolk powder, 93.07g of whole milk powder and 23.08g of maltodextrin in a three-dimensional mixer, and uniformly mixing to obtain first-grade premixed powder;
(3) adding 300.00g of milk mineral salt into the primary premixed powder, placing the mixture into a three-dimensional mixer, performing secondary mixing, and uniformly mixing to obtain secondary premixed powder which is named as mixed powder A;
(4) with vitamin D30.35g of microcapsule powder with the total amount of 1 is taken from the mixed powder A and mixed with the powder A for the 1 st time; on the basis, adding the mixed powder A with the same weight for the 2 nd mixing; and so on. Mixing the two powders completely to obtain total mixed powder;
(5) and (3) putting the total mixed powder into a powder filling and sealing machine, subpackaging by 5 g/bag, filling and sealing to obtain the powder health-care food.
Example 10
According to one embodiment of the health food for increasing bone density, the health food is a tablet and comprises the following raw materials: the bone density increasing composition and solid preparation auxiliary material described in example 3 comprise 1.44g of starch, 1.47g of hydroxypropyl methylcellulose, 1.00g of corn starch, 1.25g of polyvinylpyrrolidone and 0.50g of sorbitol.
The preparation method of the tablet health-care food comprises the following steps:
(1) weighing 15.00g of milk mineral salt, 4.18g of hydrolyzed yolk powder and vitamin D according to the prescription amount30.018g of microcapsule powder, 0.15g of whole milk powder, 1.44g of starch, 1.47g of hydroxypropyl methyl cellulose, 1.00g of corn starch, 1.25g of polyvinylpyrrolidone and 0.50g of sorbitol;
(2) 1.44g of starch, 1.00g of corn starch and 0.50g of sorbitol are put into a three-dimensional mixer to be uniformly mixed, and then 4.18g of hydrolyzed egg yolk powder and 0.15g of whole milk powder are added to obtain first-grade premixed powder;
(3) adding 15.00g of milk mineral salt into the primary premixed powder, performing secondary mixing, and uniformly mixing to obtain secondary premixed powder which is named as mixed powder A;
(4) with vitamin D30.018g of microcapsule powder is 1 in total, and equal weight of materials are taken from the mixed powder A and mixed with the powder A for the 1 st time; on the basis, adding the mixed powder A with the same weight for the 2 nd mixing; and so on. Mixing the two powders completely to obtain total mixed powder;
(5) taking 1.47g of hydroxypropyl methyl cellulose and 1.25g of polyvinylpyrrolidone as a total amount of 1, and taking materials with equal weight from the total mixed powder to mix with the mixture for the 1 st time; on the basis, adding the 2 nd mixing with the total mixed powder with the same weight; and so on. Mixing the two materials completely to obtain powder to be tabletted;
(6) and (3) tabletting the powder to be tabletted into tablets, wherein each tablet is 0.25g, so as to obtain the tablet health-care food.
Example 11
According to one embodiment of the health food for increasing bone density, the health food is a hard capsule and comprises the following raw materials: the bone density increasing composition and solid preparation auxiliary material described in example 4 comprise 0.010g of silicon dioxide, 0.30g of isolated soy protein, 0.30g of resistant dextrin, 0.60g of sodium carboxymethylcellulose and 0.40g of methylcellulose.
The preparation method of the hard capsule health food comprises the following steps:
(1) weighing 4.80g of milk mineral salt, 1.34g of hydrolyzed yolk powder and vitamin D according to the prescription amount30.0056g of microcapsule powder, 0.34g of whole milk powder, 0.010g of silicon dioxide, 0.30g of isolated soy protein, 0.30g of resistant dextrin, 0.60g of sodium carboxymethylcellulose and 0.40g of methyl cellulose;
(2) uniformly mixing 0.010g of silicon dioxide, 0.30g of isolated soy protein and 0.30g of resistant dextrin in a three-dimensional mixer, and then adding 1.34g of hydrolyzed yolk powder and 0.34g of whole milk powder to obtain first-grade premixed powder;
(3) adding 4.80g of milk mineral salt into the first-stage premixed powder, performing second-stage mixing, and uniformly mixing to obtain second-stage premixed powder which is named as mixed powder A;
(4) with vitamin D30.0056g of microcapsule powder (total amount is 1) and 1 st mixing with the same weight of the powder A; on the basis, adding the mixed powder A with the same weight for the 2 nd mixing; and so on. Mixing the two powders completely to obtain total mixed powder;
(5) taking 0.60g of sodium carboxymethylcellulose and 0.40g of methylcellulose as a total amount of 1, and taking materials with equal weight from the total mixed powder to mix with the mixed powder for the 1 st time; on the basis, adding the 2 nd mixing with the total mixed powder with the same weight; and so on. Mixing the two materials completely to obtain powder to be filled;
(6) and (3) putting the powder to be filled into a hard capsule machine, and filling No. 5 hard capsules with 0.08g per capsule to obtain the hard capsule health food.
Example 12
In this example, the health food for increasing bone density in examples 8, 10 and 11 is taken as an example to study the quality of the health food of the present invention.
In this example, 3 batches of health food preparations for increasing bone density were prepared in different formulations as described in examples 8, 10 and 11. The powder prepared in example 8 was used for organoleptic examination; each dosage form was randomly tested for functional active ingredient in 1 lot each.
(1) Sensory requirements
The sensory requirement detection results of the health food for increasing bone density by the powder of the invention are shown in table 1:
TABLE 1 sensory requirements and test results thereof
(2) Functional active ingredient
The detection results of the functional active ingredients of the health food for increasing bone density by powder, tablets and capsules are shown in table 2:
TABLE 2 index of effective components and test results thereof
The results show that the health food prepared by the mixing preparation method of the invention with the equivalent incremental mode and the multiple amplification has stable quality, and the materials with low proportion in the prescription can be uniformly mixed with other materials, thereby ensuring the uniformity of the health food in the prescription and the efficacy of the health food.
Example 13
This example takes the health food for increasing bone density in example 8 as an example, and studies the effect of the health food of the present invention in promoting bone growth.
Test materials: SD rats with weaning period of about 4 weeks are selected, cleaned, female and 70-90g in body weight. The temperature of the breeding room is 20-25 ℃, and the relative humidity is 40-60%. The feed is composed of protein, fat and carbohydrate. The drinking water is deionized water. After the rats acclimatized, the rats were fasted for 12 hours, weighed, measured for body length, and randomly bred in 4 cages, 30 rats/cage.
Dose design: the feed of rats in the blank control group is not added with any other substances. Calcium carbonate control rats were fed 580 mg/kg. Referring to the pre-test dose, the lower and upper sample dose groups were 205.6, 308.5mg/kg, respectively, which is equivalent to 0.67, 1 times the recommended dose for human.
The test method comprises the following steps: the time and mode of feeding the rats are shown in table 3. Feed and deionized water served as the rat survival base. The body weight was weighed every 10 days, 10 rats in each group were sacrificed after measuring the body length, and the bones were examined.
Statistical treatment: all data are expressed as mean ± standard deviation. The differences among the groups are processed by SPSS software and single-factor variance analysis, wherein p is less than 0.05 and has statistical significance, and p is less than 0.01 and has significant statistical significance.
TABLE 3 rat feeding time and mode
And (3) test results:
(1) the low and high dose groups of rats extended bones, inhibited osteoclast differentiation and promoted osteogenic growth compared to the blank control group in all periods of day 10, day 20 and day 30. The bone elongation of the rats in the high dose group was increased by 15% on day 30, and the difference was statistically significant (p < 0.01).
(2) The cell number proliferation rate of osteoblasts was increased to 105% for the low dose group rats on day 10, day 20, and day 30, compared to calcium carbonate control group rats, for which the difference was statistically significant (p < 0.05); the bone elongation is improved by 3.5 percent, and the difference has statistical significance (p is less than 0.05); the activity of bone alkaline phosphatase in serum is improved by 5%, and the difference has statistical significance (p is less than 0.05).
(3) The cell number proliferation rate of the osteoblasts of the rats of the high dose group increased to 160% over the entire period of days 10, 20 and 30, compared to the calcium carbonate control group rats on day 30, the difference being statistically significant (p < 0.01); the bone elongation is improved by 15 percent, and the difference has statistical significance (p is less than 0.01); the activity of bone alkaline phosphatase in serum is improved by 16%, and the difference has statistical significance (p is less than 0.01). Calcification of the bone joints was promoted as observed by electron microscopy.
(4) The rats orally take the sample of example 8, which is equivalent to 0.67 and 1 time of the dose recommended by human bodies, and the final data shows that both dose groups can promote the growth of the bones of the rats, and compared with the low dose group, the effect of the high dose group is more obvious. Indicating that the test result is positive.
And (4) test conclusion:
according to the test results, the novel composition for increasing bone density has the effect of promoting bone growth.
Example 14
This example takes the health food for increasing bone density in example 8 as an example, and studies the effect of the health food of the present invention in promoting bone growth. Experiments are designed according to the scheme of 'testing method for increasing bone mineral density' in 'health food testing and evaluation technical Specification' (2003 edition).
Test materials: SPF grade SD male healthy rats weighing 60-75g were provided by southern university of medical laboratory animal center. The laboratory is a shielding environment, the temperature is 20-26 ℃, and the relative humidity is 40-70%. The pellet feed and the basic feed powder are provided by the experimental animal center of Guangdong province. The animals were randomly fed in 5 dose groups of 10 animals/group.
The test dose is as follows: the low, medium and high sample dosage groups are respectively set as 0.25, 0.50 and 1.50g/kg BW, which are 5, 10 and 30 times of the recommended dosage of human body, and a low calcium control group (150mg/100g feed) and a calcium carbonate control group (0.58g/kg BW, calcium carbonate control group with the same level as the high dosage calcium) are additionally set.
The test method comprises the following steps: the experimental animals were fed daily ad libitum with sufficient feed and water for each mouse, and with deionized water to avoid calcium gain from the water for 3 months.
Observation indexes are as follows: general conditions (general physiological signs, appearance, behavior, stool and urine, fur, etc. in rats); femur weight (animals sacrificed after feeding for 3 months, right femur was dissected off, baked in 105 ℃ oven to constant weight, bone dry weight was weighed); bone density (measured using a dual-energy X-ray bone densitometer for the center and distal end of the left femur); bone calcium content (measured by inductively coupled plasma spectrometer).
Statistical treatment: using SPSS software to process the anova, firstly carrying out anova test according to an anova program, wherein the anova is uniform, calculating an F value, and the F value is less than F0.05, and concluding: the difference between the average numbers of all groups is not significant; f value is more than or equal to F0.05, and statistics is carried out by a pairwise comparison method of mean values between a plurality of experimental groups and a control group; carrying out appropriate variable conversion on the data which are not normal or uneven in variance, and counting by using the converted data after the requirements of normal or uniform variance are met; if the variable still does not reach the goal of being normal or uniform in variance after conversion, the statistics is carried out by using the rank sum test.
The test results are shown in table 4:
(1) general conditions are as follows: the physiological signs, behaviors, stool and urine, fur and the like of the rats are not abnormal generally.
(2) Left femoral bone density: compared with the low-calcium control group, the bone density of the distal end and the bone density of the midpoint of the left femur of each dose group of rats are increased, and the difference has statistical significance (P is less than 0.05); and compared with the calcium carbonate control group, the high-dose group has no statistical significance on the difference between the bone density at the far end of the left femur and the bone density at the midpoint (P is more than 0.05). The test sample is proved to have positive bone density test result.
(3) Calcium content of right femur: compared with the low-calcium control group, the weight of the right femur of each dose group of rats is increased, and the difference is statistically significant (P is less than 0.05); and the weight difference of the right femur in the high dose group compared with the calcium carbonate control group is not statistically significant (P > 0.05). Compared with the low-calcium control group, the calcium content of the right femur in the medium and high dose groups is increased, and the difference has statistical significance (P is less than 0.05); and compared with the calcium carbonate control group, the calcium content difference of the right femur has no statistical significance (P is more than 0.05). The test result of the bone calcium content of the test sample is positive.
Table 4 effect on femur weight, bone calcium content and bone density (n-10,)
note: each dose group was compared to a low calcium control group: p <0.01, P <0.05 test conclusion: according to judgment standard of 'test method for increasing bone mineral density' in 'health food test and evaluation technical Specification' (2003 edition), the novel composition for increasing bone mineral density has the function of increasing bone mineral density.
In summary, the milk mineral salt, the hydrolyzed yolk powder and the vitamin D are used3Micro-capsule powder and whole milk powder as raw materials; is calcium, peptide and vitamin D3Functional active component capable of dissolving calcium continuously and easily absorbed by human bodyCan be absorbed by human body to increase bone density.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (10)
1. A composition for increasing bone density, comprising the following raw materials: milk mineral salt, hydrolyzed yolk powder, and vitamin D3Micro-capsule powder and whole milk powder.
2. The composition for increasing bone density according to claim 1, wherein the following raw materials are included per 100 formulation units: 4.32-1.00 kg of milk mineral salt, 1.20-275.60 g of hydrolyzed yolk powder and vitamin D30.005-1.16 g of micro-capsule powder and 0.15-383.30 g of whole milk powder.
3. Use of the bone density increasing composition according to claim 1 or 2 for the preparation of a health food for increasing bone density.
4. A health food for increasing bone density, comprising the composition for increasing bone density according to claim 1 and an auxiliary material acceptable in the field of health foods.
5. The health food for increasing bone density according to claim 4, wherein the auxiliary materials acceptable in the field of health foods comprise solid preparation auxiliary materials and/or adhesives.
6. The health food for increasing bone density according to claim 5, comprising the following raw materials per 100 formulation units: 4.32-1.00 kg of milk mineral salt, 1.20-275.60 g of hydrolyzed yolk powder and vitamin D30.005 g-1 g of microcapsule powder.16g, 0.15-383.30 g of whole milk powder, 1.52-23.08 g of solid preparation auxiliary material and/or 0.02-2.00 g of adhesive.
7. The health food for increasing bone density according to claim 5 or 6, wherein the solid preparation excipient is at least one selected from the group consisting of soybean protein isolate, maltodextrin, corn starch and resistant dextrin; the adhesive is selected from at least one of sodium carboxymethylcellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, starch slurry, purified water, methyl cellulose, ethyl cellulose, hydroxypropyl methyl cellulose, polyvinylpyrrolidone and sucrose.
8. The health food for increasing bone density according to any one of claims 4 to 6, wherein the health food is in the form of powder, tablet or capsule.
9. The method for preparing a health food for increasing bone density according to any one of claims 4 to 8, wherein the raw materials are mixed in an equivalent incremental multiple amplification manner.
10. The manufacturing method according to claim 9, wherein the raw materials are mixed using at least one of a three-dimensional mixer, a double cone mixer, a V-type mixer, a two-dimensional mixer, a spray dryer, a fluidized bed spray dryer, a powder filling and sealing machine, a tablet press, a coating machine, a granulation machine, a hard capsule machine, and a soft capsule machine.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115399421A (en) * | 2022-07-29 | 2022-11-29 | 海南小小诺生物科技有限公司 | Solid beverage for promoting bone growth and development and preparation method thereof |
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| CN106578094A (en) * | 2016-12-31 | 2017-04-26 | 东北农业大学 | Old-aged formula milk powder for preventing osteoporosis |
| CN107535610A (en) * | 2017-10-28 | 2018-01-05 | 卜球 | A kind of formula milk and its production method for contributing to skeleton health |
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| CN103083648A (en) * | 2013-02-21 | 2013-05-08 | 汤臣倍健股份有限公司 | Troche for enhancing bone mineral density based on milk mineral salt as raw material as well as preparation method thereof |
| CN106578094A (en) * | 2016-12-31 | 2017-04-26 | 东北农业大学 | Old-aged formula milk powder for preventing osteoporosis |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115399421A (en) * | 2022-07-29 | 2022-11-29 | 海南小小诺生物科技有限公司 | Solid beverage for promoting bone growth and development and preparation method thereof |
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