CN110804573B - Bacilloid fungoides fermentation method - Google Patents
Bacilloid fungoides fermentation method Download PDFInfo
- Publication number
- CN110804573B CN110804573B CN201911232924.3A CN201911232924A CN110804573B CN 110804573 B CN110804573 B CN 110804573B CN 201911232924 A CN201911232924 A CN 201911232924A CN 110804573 B CN110804573 B CN 110804573B
- Authority
- CN
- China
- Prior art keywords
- fermentation
- culture solution
- feather
- fermenting
- degrading
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a fermentation method of bacteroides fungoides, which is characterized by comprising the following steps of: (a) Preparing a fermentation substrate, sterilizing the feathers, and adding a culture solution according to a certain proportion to obtain the fermentation substrate; (b) inoculating a strain capable of degrading the feather; inoculating the obtained fermentation substrate with a strain capable of degrading feather in a certain proportion; (c) Fermenting at a certain temperature and pH value for 20-80H; the invention provides a fermentation method of bacteroides fungoides, and provides a fermentation method capable of quickly and efficiently degrading feathers, which can quickly degrade feathers and can obtain amino acid and other nutritional ingredients after fermentation is completed, so that the problem that feathers are difficult to treat is solved, and the environment is not polluted basically in the fermentation process.
Description
Technical Field
The invention relates to the technical field of bioengineering, in particular to a fermentation method of bacillus mycoides.
Background
In recent years, the poultry breeding industry in China is rapidly developed, feathers can account for 5% -7% of the weight of broiler chickens, the yield per year exceeds 100 million tons, most feathers are not utilized and are usually buried or burned, so that the huge waste of resources is caused, the environment is seriously polluted, even diseases are spread, the newcastle disease, the air sac inflammation, the keratoconjunctivitis and the like are caused, in addition, harmful gases are released by burning, and the discharged ammonia and the malodorous hydrogen sulfide cause health problems such as headache, nausea, diarrhea and the like. At present, the feathers are treated by physicochemical methods such as high temperature and high pressure, strong acid and strong alkali, extrusion and expansion and the like, the process is complex, the energy consumption is high, and the environmental pollution is easy to cause. Therefore, if the feather can be recycled, the environmental pollution is reduced, the resources are saved, and the sustainable development of the resources in China is promoted.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and provides a fermentation method of bacillus mycoides for fermentation by using feathers as raw materials so as to efficiently degrade the feathers.
The technical scheme of the invention is as follows:
a fermentation method of Bacteroides mycoides comprises the following steps:
(a) Making fermentation base material
Sterilizing the feathers, and adding a culture solution according to a certain proportion to obtain a fermentation substrate;
(b) Inoculating strain capable of degrading feather
Inoculating the obtained fermentation substrate with a strain capable of degrading feather in a certain proportion;
(c) Fermenting the mixture
Fermenting at certain temperature and pH value for 20-80H.
Preferably, the culture medium contains a carbon source in an amount of 2 to 20g per liter of the culture medium.
Further, the carbon source is glucose or soluble starch or sucrose or fructose or maltose, and fructose is preferably selected as the carbon source.
Preferably, the culture medium contains a nitrogen source in an amount of 2 to 20g per liter of the culture medium.
Further, the nitrogen source is beef powder, yeast powder, corn steep liquor, peptone, casein, ammonium sulfate or ammonium nitrate, and preferably corn steep liquor is used as the carbon source.
Preferably, the culture solution also contains inorganic salt, and each liter of the culture solution contains 0.05-0.5g of the inorganic salt.
Further, the inorganic salt is anhydrous magnesium sulfate, copper sulfate, manganese sulfate, zinc sulfate, calcium chloride, ferrous sulfate or aluminum chloride, and calcium chloride is preferably selected.
Preferably, the culture solution is added according to a certain proportion and is feather by mass percent: culture solution =1:100-10:1.
Preferably, the nucleotide sequence of the feather-degrading strain is shown in SEQ ID NO.1, the strain is deposited with the number GDMCC 60687 and is deposited by Guangdong province microbial strain collection center with the preservation date of 2019, 6 and 13 days, the preservation address is No. 59, no. 5, of the Michelia furiosa No. 100, guangzhou city, and the classification type is Bacillus paramycoides.
Preferably, the temperature is 25-45 degrees.
Preferably, the pH is 5 to 8.
Preferably, the fermentation also comprises an oxygen increasing and increasing condition, and a proper amount of oxygen is added in the fermentation process for the growth and the propagation of strains.
The invention has the beneficial effects that: the invention provides a fermentation method of bacteroides fungoides, and provides a fermentation method capable of quickly and efficiently degrading feathers, which can quickly degrade feathers and can obtain amino acid and other nutritional ingredients after fermentation is completed, so that the problem that feathers are difficult to treat is solved, and the environment is not polluted basically in the fermentation process.
Drawings
FIG. 1 is an experimental diagram of Bacillus mycoides of the present invention before fermentation
FIG. 2 is a graph showing the results of fermentation of Bacillus mycoides 20H according to the present invention
FIG. 3 is a graph showing the results of fermentation of Bacillus mycoides 40H according to the present invention
FIG. 4 is a graph showing the results of fermentation of Bacillus mycoides of the present invention for 60H
FIG. 5 is a report of the test of crude protein after 40H fermentation of Bacillus mycoides of the present invention
FIG. 6 is a report of amino acid tests after 40H fermentation of Bacillus mycoides according to the present invention
Detailed Description
The following describes an implementation structure of the present invention with reference to the drawings.
In order to more clearly explain the objects, technical solutions and points of the present invention, the present invention is further described in detail with reference to the accompanying drawings. The specific embodiments described herein are merely illustrative of the invention.
Example 1
As shown in fig. 1 to 2, the technical solution of the present invention is: the materials and experimental method adopted by the invention comprise the following steps:
a fermentation method of Bacillussinoides comprises the following steps:
(a) Making fermentation base material
Sterilizing the feathers, and adding a culture solution according to a certain proportion to obtain a fermentation substrate; the culture solution contains a carbon source, wherein each liter of the culture solution contains 2g of carbon source, and the carbon source is glucose; the culture solution contains a nitrogen source, wherein each liter of the culture solution contains 2g of nitrogen source, and the nitrogen source is beef powder; the culture solution also contains inorganic salt, wherein each liter of the culture solution contains 0.05g of the inorganic salt, and the inorganic salt is anhydrous magnesium sulfate.
(b) Inoculating strain capable of degrading feather
Inoculating the obtained fermentation substrate with a strain capable of degrading feather in a certain proportion; the feather is added with culture solution according to a certain proportion by mass percent: culture solution =1:100, the nucleotide sequence of the strain capable of degrading the feathers is shown as SEQ ID NO.1, the preservation number of the strain is GDMCC 60687, the strain is preserved by Guangdong provincial microorganism strain preservation center, the preservation date is 2019, 6 and 13 days, the preservation address is No. 59 Lou 5 of Michelia Tokyo No. 100 in Guangzhou city, and the classification is Bacillus paramycoides.
(c) Fermenting the mixture
Fermenting for 20H at a certain temperature and pH value, wherein the temperature is 25 ℃, and the pH value is 5.
In order to better promote the growth of the bacillus paratungensis, the fermentation also comprises an oxygen increasing and increasing condition, and a proper amount of oxygen is added in the fermentation process for the growth and the propagation of strains.
FIG. 2 is a graph after fermentation is completed, which illustrates that the Bacillus mycoides fermentation method of the present invention can degrade feathers rapidly and efficiently, solves the problem that feathers are difficult to treat, and basically causes no pollution to the environment during fermentation.
Example 2
As shown in fig. 1, fig. 3, fig. 5 and fig. 6, the technical solution of the present invention is: the materials and experimental method adopted by the invention comprise the following steps:
a fermentation method of Bacteroides mycoides comprises the following steps:
(a) Making fermentation base material
Adding feather into culture solution according to a certain proportion, and sterilizing to obtain fermentation substrate; the culture solution contains a carbon source, wherein each liter of the culture solution contains 10g of carbon source, and the carbon source is soluble starch; the culture solution contains a nitrogen source, wherein each liter of the culture solution contains a nitrogen source of 10g and yeast powder of the nitrogen source; the culture solution also contains inorganic salt, wherein each liter of the culture solution contains 0.2g of the inorganic salt, and the inorganic salt is copper sulfate.
(b) Inoculating strain capable of degrading feather
Inoculating the obtained fermentation substrate with a strain capable of degrading feather in a certain proportion; the feather is added with culture solution according to a certain proportion by mass percent: the culture solution =1, the nucleotide sequence of the feather-degrading strain is shown in SEQ ID No.1, the strain is deposited with the number GDMCC 60687 and is deposited by Guangdong province microbial strain collection center with the preservation date of 2019, 6-13 months, the preservation address of No. 59, no. 5, of the college No. 100 of the Pieli Zhongluo, guangzhou city, and the classification of the feather-degrading strain is Bacillus paramycoides.
(c) Fermenting the mixture
Fermenting for 40H at a certain temperature and pH value, wherein the temperature is 35 ℃, and the pH value is 6.
In order to better promote the growth of the bacillus paratungensis, the fermentation also comprises an oxygen increasing and increasing condition, and a proper amount of oxygen is added in the fermentation process for the growth and the propagation of strains.
FIG. 3 is a graph after fermentation, which illustrates that the Bacillus mycoides fermentation method of the present invention can degrade feathers rapidly and efficiently, the fermentation speed is fast, the fermentation liquid after fermentation is inspected, as shown in FIG. 5, the fermentation liquid contains 0.98% crude protein; as shown in figure 6, the fermentation liquor contains 31.9g/L of organic matter, 4.02g/L of free amino acid, 6.2g/L of potassium and other components which are decomposed from the feathers, thereby further proving that the strain has the effect of efficiently degrading the feathers, and meanwhile, the fermentation method can be used for obtaining the components such as the amino acid, effectively recycling the feathers, reducing the waste of the feathers, solving the problem that the feathers are difficult to treat, and basically causing no pollution to the environment in the fermentation process.
Example 3
As shown in FIG. 1 and FIG. 4, a fermentation method of Bacteroides mycoides comprises the following steps:
(a) Making fermentation base material
Sterilizing the feathers, and adding a culture solution according to a certain proportion to obtain a fermentation substrate; the culture solution contains a carbon source, wherein each liter of the culture solution contains 15g of carbon source, namely sucrose; the culture solution contains a nitrogen source, wherein each liter of the culture solution contains 15g of nitrogen source, and the nitrogen source is corn steep liquor; the culture solution also contains inorganic salt, wherein each liter of the culture solution contains 0.3g of inorganic salt, and the inorganic salt is manganese sulfate.
(b) Inoculating strain capable of degrading feather
Inoculating the obtained fermentation substrate with a strain capable of degrading feather in a certain proportion; the feather is added with culture solution according to a certain proportion by mass percent: the culture solution =1, the nucleotide sequence of the feather-degrading strain is shown in SEQ ID No.1, the strain is deposited with the number GDMCC 60687 and is deposited by Guangdong province microbial strain collection center with the preservation date of 2019, 6-13 months, the preservation address of No. 59, no. 5, of the college No. 100 of the Pieli Zhongluo, guangzhou city, and the classification of the feather-degrading strain is Bacillus paramycoides.
(c) Fermenting the mixture
Fermenting for 60H at a certain temperature and pH value, wherein the temperature is 40 ℃, and the pH value is 7.
In order to better promote the growth of the bacillus paratungensis, the fermentation also comprises an oxygen increasing and increasing condition, and a proper amount of oxygen is added in the fermentation process for the growth and the propagation of strains.
Example 4
A fermentation method of Bacteroides mycoides comprises the following steps:
(a) Making fermentation base material
Adding feather into culture solution according to a certain proportion, and sterilizing to obtain a fermentation substrate; the culture solution contains a carbon source, wherein each liter of the culture solution contains 20g of carbon source, and the carbon source is fructose; the culture solution contains a nitrogen source, wherein each liter of the culture solution contains 20g of nitrogen source, and the nitrogen source is peptone; the culture solution also contains inorganic salt, wherein each liter of the culture solution contains 0.5g of the inorganic salt, and the inorganic salt is calcium chloride.
(b) Inoculating strain capable of degrading feather
Inoculating the obtained fermentation substrate with a strain capable of degrading feather in a certain proportion; the feather is added with culture solution according to a certain proportion by mass percent: the culture solution =10, the nucleotide sequence of the feather-degrading strain is shown in SEQ ID No.1, the strain is deposited with the number GDMCC 60687 and is deposited by Guangdong province microbial strain collection center with the preservation date of 2019, 6-13 months, the preservation address of No. 59, no. 5, of the college No. 100 of the Pieli Zhongluo, guangzhou city, and the classification of the feather-degrading strain is Bacillus paramycoides.
(c) Fermenting the mixture
Fermenting for 80H at a certain temperature and pH value, wherein the temperature is 45 ℃, and the pH value is 8.
In order to better promote the growth of the bacillus paratungensis, the fermentation also comprises an oxygen increasing and increasing condition, and a proper amount of oxygen is added in the fermentation process for the growth and the propagation of strains.
The above description is only a specific embodiment of the present invention. The scope of the present invention is not limited thereto, and any changes or simple substitutions which are not thought of through creative efforts should be covered within the scope of the present invention.
SEQUENCE LISTING
<110> Guangxi national university
<120> fermentation method of Bacillussiliensis
<130> 2019
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1409
<212> DNA
<213> Bacteroides (Bacillus paramycoides)
<400> 1
tgcagtcgag cgaatggatt aagagcttgc tcttatgaag ttagcggcgg acgggtgagt 60
aacacgtggg taacctgccc ataagactgg gataactccg ggaaaccggg gctaataccg 120
gataacattt tgaaccgcat ggttcgaaat tgaaaggcgg cttcggctgt cacttatgga 180
tggacccgcg tcgcattagc tagttggtga ggtaacggct caccaaggca acgatgcgta 240
gccgacctga gagggtgatc ggccacactg ggactgagac acggcccaga ctcctacggg 300
aggcagcagt agggaatctt ccgcaatgga cgaaagtctg acggagcaac gccgcgtgag 360
tgatgaaggc tttcgggtcg taaaactctg ttgttaggga agaacaagtg ctagttgaat 420
aagctggcac cttgacggta cctaaccaga aagccacggc taactacgtg ccagcagccg 480
cggtaatacg taggtggcaa gcgttatccg gaattattgg gcgtaaagcg cgcgcaggtg 540
gtttcttaag tctgatgtga aagcccacgg ctcaaccgtg gagggtcatt ggaaactggg 600
agacttgagt gcagaagagg aaagtggaat tccatgtgta gcggtgaaat gcgtagagat 660
atggaggaac accagtggcg aaggcgactt tctggtctgt aactgacact gaggcgcgaa 720
agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgagtgct 780
aagtgttaga gggtttccgc cctttagtgc tgaagttaac gcattaagca ctccgcctgg 840
ggagtacggc cgcaaggctg aaactcaaag gaattgacgg gggcccgcac aagcggtgga 900
gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca tcctctgaca 960
accctagaga tagggcttct ccttcgggag cagagtgaca ggtggtgcat ggttgtcgtc 1020
agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt gatcttagtt 1080
gccatcatta agttgggcac tctaaggtga ctgccggtga caaaccggag gaaggtgggg 1140
atgacgtcaa atcatcatgc cccttatgac ctgggctaca cacgtgctac aatggacggt 1200
acaaagagct gcaagaccgc gaggtggagc taatctcata aaaccgttct cagttcggat 1260
tgtaggctgc aactcgccta catgaagctg gaatcgctag taatcgcgga tcagcatgcc 1320
gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgag agtttgtaac 1380
acccgaagtc ggtggggtaa ccttttgga 1409
Claims (11)
1. A Bacilloid (B. Mycoides)Bacillus paramycoides) A fermentation process, comprising the steps of:
(a) Making fermentation base material
Adding feather into culture solution according to a certain proportion for sterilization to obtain a fermentation substrate;
(b) Inoculating strain capable of degrading feather
Inoculating the obtained fermentation substrate with a strain capable of degrading the feather in a certain proportion, wherein the preservation number of the strain is GDMCC No:60687, which is preserved by Guangdong province microorganism strain preservation center, wherein the preservation date is 2019, 6 and 17 days, and the preservation address is No. 59 building 5 of Jie No. 100 college of Jieli Zhonglu, guangzhou city;
(c) Fermenting the mixture
Fermenting for 20-80 h at a certain temperature and p H value.
2. The method of claim 1, wherein the culture solution contains a carbon source in an amount of 2-20 g/L.
3. The method of claim 2, wherein the carbon source is glucose or soluble starch or sucrose or fructose.
4. The method of claim 1, wherein the culture medium contains a nitrogen source in an amount of 2-20g per liter.
5. The method for fermenting Bacteroides mushroom according to claim 4, wherein the nitrogen source is beef powder, yeast powder or corn steep liquor or ammonium nitrate.
6. The method of claim 1, wherein the culture medium further comprises inorganic salts, and the amount of inorganic salts is 0.05-0.5g per liter of the culture medium.
7. The method for fermenting Bacillus mycoides according to claim 6, wherein said inorganic salt is anhydrous magnesium sulfate or copper sulfate or manganese sulfate or calcium chloride.
8. The method for fermenting Bacteroides mushroom according to claim 1, wherein the culture solution is added according to a certain proportion according to the mass percentage of feather: culture solution =1:100-10:1.
9. The method of claim 1, wherein the temperature is about 25-45 degrees.
10. The method of claim 1, wherein the p H value is 5-8.
11. The method as claimed in claim 1, wherein the fermentation further comprises oxygen enrichment, and a proper amount of oxygen is added during the fermentation process for the growth and propagation of the bacterial species.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911232924.3A CN110804573B (en) | 2019-12-05 | 2019-12-05 | Bacilloid fungoides fermentation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911232924.3A CN110804573B (en) | 2019-12-05 | 2019-12-05 | Bacilloid fungoides fermentation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110804573A CN110804573A (en) | 2020-02-18 |
| CN110804573B true CN110804573B (en) | 2022-10-21 |
Family
ID=69492506
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911232924.3A Active CN110804573B (en) | 2019-12-05 | 2019-12-05 | Bacilloid fungoides fermentation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110804573B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110819572B (en) * | 2019-12-05 | 2022-10-21 | 广西民族大学 | Bacillusmycoides |
| CN116218739B (en) * | 2023-03-30 | 2024-06-04 | 中国科学院近代物理研究所 | Bacillus paramycoides and application thereof in sewage treatment |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104745504A (en) * | 2015-03-06 | 2015-07-01 | 华南理工大学 | Bacillus cereus from deep sea-based sources and application thereof in producing neutral proteinase |
| CN109355228A (en) * | 2018-11-20 | 2019-02-19 | 南京林业大学 | One plant of forest rhizosphere bacteria intends bacillus mycoides JYZ-SD5 and its application |
| CN110819572A (en) * | 2019-12-05 | 2020-02-21 | 广西民族大学 | Bacillusbacteroides |
| CN110839758A (en) * | 2019-12-05 | 2020-02-28 | 广西民族大学 | Feed |
| CN110922238A (en) * | 2019-12-05 | 2020-03-27 | 广西民族大学 | Liquid fertilizer |
| CN112646746A (en) * | 2021-01-04 | 2021-04-13 | 济南大学 | Bacillus firmus for efficiently degrading donkey hair |
| WO2021247915A1 (en) * | 2020-06-03 | 2021-12-09 | Sound Agriculture Company | Compounds and methods for stimulating plants |
| CN113897306A (en) * | 2021-09-13 | 2022-01-07 | 广西民族大学 | Compound microbial agent and application thereof |
-
2019
- 2019-12-05 CN CN201911232924.3A patent/CN110804573B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104745504A (en) * | 2015-03-06 | 2015-07-01 | 华南理工大学 | Bacillus cereus from deep sea-based sources and application thereof in producing neutral proteinase |
| CN109355228A (en) * | 2018-11-20 | 2019-02-19 | 南京林业大学 | One plant of forest rhizosphere bacteria intends bacillus mycoides JYZ-SD5 and its application |
| CN110819572A (en) * | 2019-12-05 | 2020-02-21 | 广西民族大学 | Bacillusbacteroides |
| CN110839758A (en) * | 2019-12-05 | 2020-02-28 | 广西民族大学 | Feed |
| CN110922238A (en) * | 2019-12-05 | 2020-03-27 | 广西民族大学 | Liquid fertilizer |
| WO2021247915A1 (en) * | 2020-06-03 | 2021-12-09 | Sound Agriculture Company | Compounds and methods for stimulating plants |
| CN112646746A (en) * | 2021-01-04 | 2021-04-13 | 济南大学 | Bacillus firmus for efficiently degrading donkey hair |
| CN113897306A (en) * | 2021-09-13 | 2022-01-07 | 广西民族大学 | Compound microbial agent and application thereof |
Non-Patent Citations (6)
| Title |
|---|
| Jianjun Ren等.Biodegradation of acephate by Bacillus paramycoides NDZ and its degradation pathway.《World Journal of Microbiology and Biotechnology》.2020,第36卷(第10期), * |
| Shen,N.等.Bacillus paramycoides strain Gxun-30 16S ribosomal RNA gene, partial sequence.《GenBank》.2020, * |
| Zeiad Moussa等.Innovative Artificial-Intelligence- Based Approach for the Biodegradation of Feather Keratin by Bacillus paramycoides, and Cytotoxicity of the Resulting Amino Acids.《Front Microbiol》.2021,第12卷 * |
| 张奇等.芽孢杆菌L_4菌株角蛋白酶的酶学性质研究.《农业环境科学学报》.2009,第28卷(第10期), * |
| 张妮等.一株海洋来源高效产角蛋白酶菌株的筛选、鉴定及其酶学性质研究.《食品与发酵工业》.2020,第46卷(第18期), * |
| 张红岩等.拟蕈状芽孢杆菌Gxun-30产角蛋白酶液体发酵条件优化.《食品与发酵工业》.2020,第47卷(第4期), * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110804573A (en) | 2020-02-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110804573B (en) | Bacilloid fungoides fermentation method | |
| WO2009070966A1 (en) | Microorganisms, microbial phosphate fertilizers and methods for preparing such microbial phosphate fertilizers | |
| CN106636226B (en) | Method for preparing butanol by fermenting lignocellulose | |
| CN112011491B (en) | Kitchen waste degrading agent | |
| CN107130001B (en) | A kind of method of coculture and its wheat stalk methane phase that ferments | |
| CN113046278B (en) | Composite microbial agent for fermenting and degrading cellulose in straws | |
| Cheng et al. | Continuous pullulan fermentation in a biofilm reactor | |
| CN109095955A (en) | A kind of preparation method and applications of magnesium salts modification biological charcoal | |
| CN112806486A (en) | Ruminant fermented feed and preparation method thereof | |
| CN102617198B (en) | Microbial fertilizer for promoting plant growth and culture method of microbial fertilizer | |
| CN105255771A (en) | Staphylococcus succinus for generating collagenase and application thereof | |
| CN110713939A (en) | Strain for degrading lignocellulose source inhibitor under extremely low pH condition and application | |
| CN109650957A (en) | A kind of superhigh temperature pretreatment compost method reducing nitrogen gas loss | |
| CN110839758B (en) | Feed | |
| CN106987572A (en) | A kind of method that anaerobic fermentation maize straw produces zytase | |
| CN113481103B (en) | Penicillium griseofulvum (L.) Gray | |
| CN110055195A (en) | The microbial strains BF-1801 of one high-efficiency degradation cellulose | |
| CN109797121A (en) | The microbial strains LM-1801 of one plant of degraded cellulose and its application | |
| WO2022104772A1 (en) | Cyanobacteria composting composite microbial agent and preparation method therefor | |
| CN108913629B (en) | Bacterium for producing cellulase, preparation method and application thereof | |
| WO2024139079A1 (en) | Lactobacillus plantarum and use thereof in preparation of sesame meal with low phytic acid, high acid-soluble protein and high organic acid | |
| CN103451123A (en) | Macrococcus caseolyticus and preparation method as well as application thereof | |
| CN101838620B (en) | Bacillus subtilis and alkali-resisting and salt-resisting oil field fracturing enzyme and application thereof | |
| CN114262676B (en) | Method for efficiently degrading feather keratin to produce amino acid by using bacillus | |
| CN107177537B (en) | Bacterial strains, microbial agents and uses thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |