CN110922238A - Liquid fertilizer - Google Patents
Liquid fertilizer Download PDFInfo
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- CN110922238A CN110922238A CN201911233283.3A CN201911233283A CN110922238A CN 110922238 A CN110922238 A CN 110922238A CN 201911233283 A CN201911233283 A CN 201911233283A CN 110922238 A CN110922238 A CN 110922238A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- General Chemical & Material Sciences (AREA)
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- Fertilizers (AREA)
Abstract
The invention relates to a liquid fertilizer which is characterized by comprising the following steps: (a) preparing a fermentation base material: adding feather into culture medium according to a certain proportion, and sterilizing to obtain fermentation substrate; (b) inoculating a strain capable of degrading the feather, and inoculating a certain content of a strain capable of degrading the feather to the obtained fermentation substrate; (c) fermenting under the condition of proper strain growth, and after fermenting for a period of time, completing fermentation to obtain fermentation liquor; the liquid fertilizer provided by the invention has a good promotion effect on plant growth, is mainly prepared from feathers serving as raw materials, effectively recycles the feathers, reduces the waste of the feathers, solves the problem that the feathers are difficult to treat, and basically does not pollute the environment in the production process of the fertilizer.
Description
Technical Field
The invention relates to the technical field of bioengineering, in particular to a liquid fertilizer.
Background
In recent years, the poultry breeding industry in China is rapidly developed, feathers can account for 5% -7% of the weight of broiler chickens, the yield per year exceeds 100 million tons, most feathers are not utilized and are usually buried or burned, so that the huge waste of resources is caused, the environment is seriously polluted, even diseases are spread, the newcastle disease, the air sac inflammation, the keratoconjunctivitis and the like are caused, in addition, harmful gases are released by burning, and the discharged ammonia and the malodorous hydrogen sulfide cause health problems such as headache, nausea, diarrhea and the like. At present, the feathers are treated by physicochemical methods such as high temperature and high pressure, strong acid and strong alkali, extrusion and expansion and the like, the process is complex, the energy consumption is high, and the environmental pollution is easy to cause. Therefore, if the feather can be recycled, the environmental pollution is reduced, the resources are saved, and the sustainable development of the resources in China is promoted.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and provides a liquid fertilizer by using feathers as raw materials and utilizing microorganisms for fermentation.
The technical scheme of the invention is as follows:
a liquid fertilizer comprising the steps of:
(a) making fermentation base material
Adding feather into culture medium according to a certain proportion, and sterilizing to obtain fermentation substrate;
(b) inoculating strain capable of degrading feather
Inoculating the obtained fermentation substrate with a certain content of strain capable of degrading feather, wherein the nucleotide sequence of the strain is shown as SEQ ID NO. 1;
(c) fermenting the mixture
Fermenting under the condition suitable for the growth of the strains, and after fermenting for a period of time, completing the fermentation to obtain the fermentation liquor.
Preferably, the culture medium is water: k2HPO 4: KH2PO 4: NaCl: MgSO4 ═ 150: 10: 5: 2: 1-1000:10:0.5:0.5:0.1.
Preferably, the culture medium is added according to a certain proportion, and the feather is added according to the mass percentage: medium 1: 200-1:5.
Further, the nucleotide sequence of the strain is shown as SEQ ID NO.1, and the preservation number of the strain is GDMCCNo: 60687, deposited by Guangdong province microorganism culture collection center, with the date of 2019, 6.13.7, the address of No. 59, No. 5, of the institute of Torilow 100, Guangzhou city, and the classification category of Bacillus paramycoides, wherein the literal name is Bacillus mycoides.
Preferably, the conditions suitable for growth of the strain include controlling the temperature, wherein the temperature is 25-45 ℃.
Preferably, the conditions suitable for growth of the bacterial species include controlling the pH, said pH being in the range of 5 to 8.
Preferably, the period of time is from 24H to 80H.
Preferably, the liquid fertilizer further comprises a drying step, wherein the drying step is performed on the feathers before the feathers are sterilized.
Preferably, the liquid fertilizer further comprises a crushing step of crushing the dried feathers.
Preferably, the liquid fertilizer further comprises a filtering step, wherein the fermentation liquor is filtered to obtain the liquid fertilizer.
Further, the fertilizer can be compounded into other types of fertilizers on the basis of liquid fertilizers, and other mineral elements such as magnesium are added.
The invention has the beneficial effects that: the liquid fertilizer provided by the invention has a good promotion effect on plant growth, is mainly prepared from feathers serving as raw materials, effectively recycles the feathers, reduces the waste of the feathers, solves the problem that the feathers are difficult to treat, and basically does not pollute the environment in the production process of the fertilizer.
Drawings
FIG. 1 is a set of experimental drawings for promoting the growth of wheat by using the liquid fertilizer of the invention
FIG. 2 is a bar graph of wheat plant height measurement
FIG. 3 is a set of experimental drawings for promoting corn growth by using the liquid fertilizer of the invention
FIG. 4 is a column diagram for measuring plant height of corn
FIG. 5 is a diagram of experiment for promoting growth of pepper by using the liquid fertilizer of the present invention
Detailed Description
The following describes an implementation structure of the present invention with reference to the drawings.
In order to more clearly explain the objects, technical solutions and points of the present invention, the present invention is further described in detail with reference to the accompanying drawings. The specific embodiments described herein are merely illustrative of the invention.
Example 1
As shown in fig. 1 to 2, the technical solution of the present invention is: the materials and experimental method adopted by the invention comprise the following steps:
a liquid fertilizer comprising the steps of:
(a) making fermentation base material
Drying, crushing and sterilizing the feathers, and adding the feathers into a culture medium according to a certain proportion, wherein the proportion is that the feathers are in percentage by mass: medium 1: 200 to obtain the fermentation substrate. Wherein the culture medium comprises the following components in percentage by mass: k2HPO 4: KH2PO 4: NaCl: MgSO4 ═ 150: 10: 5: 2:1, the function of the culture medium is to provide nutrient substances for the growth of strains.
(b) Inoculating strain capable of degrading feather
Inoculating the obtained fermentation substrate with a certain content of strain capable of degrading feather, wherein the nucleotide sequence of the strain is shown as SEQ ID NO.1, and the preservation number of the strain is GDMCC No: 60687, deposited by Guangdong province microorganism strain collection center, the date of deposit is 2019, 6.13.7, the address of deposit is No. 59, 5, of the institute of Torilow, Middleyao, 100, and the classification is Bacillus paramycoides.
(c) Fermenting the mixture
Fermenting under the condition of proper strain growth, wherein the condition of proper strain growth comprises temperature control and pH value control, the temperature is 25 ℃, and the pH value is 5.
Fermenting for 24H to obtain fermentation liquor.
And filtering the fermentation liquor, and removing filter residues to obtain a liquid fertilizer stock solution.
The obtained liquid fertilizer stock solution was subjected to a test,
the method comprises the steps of carrying out surface sterilization on wheat (30 s of 75% alcohol, 3 times of sterile water washing, 10min of 1% sodium hypochlorite sterilization and 5 times of sterile water washing), then carrying out sterile soaking for 2h, putting wheat seeds in dark for moisturizing for 12h by using gauze, then carrying out culture in light until the seeds germinate, selecting the seeds with consistent growth vigor, transferring the seeds into plastic pots filled with a sterilized culture medium and vermiculite in a mass ratio of 2:1, wherein each pot contains 400g of 6 seedlings of wheat. Adding 4mL of liquid fertilizer stock solution with different dilution times (stock solution, 5-time dilution, 10-time dilution and 20-time dilution) into each pot, inoculating bacteria for 1 time every 10 days, taking inoculated equivalent un-inoculated culture medium as a control, repeating the treatment for 3 times, randomly grouping and arranging, placing in a greenhouse and culturing under natural illumination. Data determination is carried out 40 days after inoculation, the results are averaged, the growth diagram of wheat is shown in figure 1, the corresponding height measurement column diagram is shown in figure 2, and as is apparent from figures 1 and 2, the height of a wheat plant planted by applying the stock solution of the liquid fertilizer is 18cm higher than that of a wheat plant in a control group and is 16cm, so that the wheat growth is promoted by adopting the stock solution of the liquid fertilizer, wherein the height of the wheat plant planted by diluting the liquid fertilizer by 5 times is the highest and reaches 25cm, the height of the wheat plant planted by diluting the liquid fertilizer by 10 times and 20 times reaches 24cm, and is 8-9cm higher than that of the wheat plant in the control group and is 16cm higher than that of the wheat plant in the control group, so that the wheat growth promotion effect is realized by using the liquid fertilizer.
Example 2
As shown in fig. 3 to 4, the technical solution of the present invention is: the materials and experimental method adopted by the invention comprise the following steps:
a liquid fertilizer comprising the steps of:
(a) making fermentation base material
Drying, crushing and sterilizing the feathers, and adding the feathers into a culture medium according to a certain proportion, wherein the proportion is that the feathers are in percentage by mass: medium 1: 80, obtaining the fermentation substrate. Wherein the culture medium comprises the following components in percentage by mass: k2HPO 4: KH2PO 4: NaCl: MgSO4 ═ 500: 10: 3: 2: 0.5, the function of the culture medium is to provide nutrient substances for the growth of the strains.
(b) Inoculating strain capable of degrading feather
Inoculating the obtained fermentation substrate with a certain content of strain capable of degrading feather, wherein the nucleotide sequence of the strain is shown as SEQ ID NO.1, and the preservation number of the strain is GDMCC No: 60687, deposited by Guangdong province microorganism strain collection center, the date of deposit is 2019, 6.13.7, the address of deposit is No. 59, 5, of the institute of Torilow, Middleyao, 100, and the classification is Bacillus paramycoides.
(c) Fermenting the mixture
Fermenting under the condition of proper strain growth, wherein the condition of proper strain growth comprises temperature control and pH value control, the temperature is 35 ℃, and the pH value is 6.5.
Fermenting for 50H to obtain fermentation liquor.
And filtering the fermentation liquor, and removing filter residues to obtain a liquid fertilizer stock solution.
The obtained liquid fertilizer stock solution was subjected to a test,
the method comprises the steps of performing surface sterilization on corn (30 s of 75% alcohol, 3 times of sterile water washing, 10min of 1% sodium hypochlorite sterilization and 5 times of sterile water washing), then performing sterile soaking for 2h, placing corn seeds in dark for gauze moisture preservation for 12h, then performing culture in light until the seeds germinate, selecting the seeds with consistent growth vigor, transferring the seeds into plastic pots filled with sterilized culture substrates and mixed vermiculite according to the mass ratio of 2:1, wherein each pot contains 400g of corn seedlings, and each pot contains 6 corn seedlings. Adding 4mL of liquid fertilizer stock solution with different dilution times (stock solution, 5-time dilution, 10-time dilution and 20-time dilution) into each pot, inoculating bacteria for 1 time every 10 days, taking inoculated equivalent un-inoculated culture medium as a control, repeating the treatment for 3 times, randomly grouping and arranging, placing in a greenhouse and culturing under natural illumination. Data determination is carried out 40 days after inoculation, the results are averaged, the growth diagram of the corn is shown in figure 3, the corresponding height measurement column diagram is shown in figure 4, and as is apparent from figures 3 and 4, the height of the corn plant planted by applying the stock solution of the liquid fertilizer is 50.8cm higher than that of the wheat plant in the control group by 46cm, which shows that the corn plant planted by applying the stock solution of the liquid fertilizer has certain promotion effect on the growth of the corn, wherein the height of the corn plant planted by diluting the liquid fertilizer by 5 times is the highest, reaches 66cm higher than that of the corn plant in the control group by nearly 20cm, the height of the corn plant planted by diluting the liquid fertilizer by 10 times reaches 65.3cm higher than that of the corn plant in the control group by 19cm, the height of the corn plant planted by diluting the liquid fertilizer by 20 times reaches 60.8cm higher than that of the corn plant in the control group by nearly 15cm, thereby illustrating the growth promoting effect of the liquid fertilizer on the corn.
Example 3
As shown in fig. 5, the technical solution of the present invention is: the materials and experimental method adopted by the invention comprise the following steps:
a liquid fertilizer comprising the steps of:
(a) making fermentation base material
Drying, crushing and sterilizing the feathers, and adding the feathers into a culture medium according to a certain proportion, wherein the proportion is that the feathers are in percentage by mass: medium 1: and 5, obtaining the fermentation substrate. Wherein the culture medium comprises the following components in percentage by mass: k2HPO 4: KH2PO 4: NaCl: MgSO4 is 1000:10:0.5:0.5:0.1, and the function of the culture medium is to provide nutrient for the growth of the strain.
(b) Inoculating strain capable of degrading feather
Inoculating the obtained fermentation substrate with a certain content of strain capable of degrading feather, wherein the nucleotide sequence of the strain is shown as SEQ ID NO.1, and the preservation number of the strain is GDMCC No: 60687, deposited by Guangdong province microorganism strain collection center, the date of deposit is 2019, 6.13.7, the address of deposit is No. 59, 5, of the institute of Torilow, Middleyao, 100, and the classification is Bacillus paramycoides.
(c) Fermenting the mixture
Fermenting under the condition of proper strain growth, wherein the condition of proper strain growth comprises temperature control and pH value control, the temperature is 45 ℃, and the pH value is 8.
Fermenting for 80H to obtain fermentation liquor.
And filtering the fermentation liquor, and removing filter residues to obtain a liquid fertilizer stock solution.
The obtained liquid fertilizer stock solution was subjected to a test,
the method comprises the steps of performing surface sterilization on the peppers (30 s of 75% alcohol, 3 times of sterile water washing, 10min of 1% sodium hypochlorite sterilization, 5 times of sterile water washing), then performing sterile soaking for 2h, placing the pepper seeds in dark, keeping moisture for 12h by gauze, then performing culture in light until the seeds germinate, selecting the seeds with consistent growth vigor, transferring the seeds into plastic pots filled with a sterilized culture medium and vermiculite in a mass ratio of 2:1, wherein each pot contains 400g of pepper seedlings, and each pot contains 6 seedlings. Adding 4mL of liquid fertilizer stock solution with different dilution times (stock solution, 5-time dilution, 10-time dilution and 20-time dilution) into each pot, inoculating bacteria for 1 time every 10 days, taking inoculated equivalent un-inoculated culture medium as a control, repeating the treatment for 3 times, randomly grouping and arranging, placing in a greenhouse and culturing under natural illumination. Data are measured after 40 days of inoculation, the results are averaged, the growth chart of the pepper is shown in fig. 5, and as is obvious from fig. 5, the growth situation of the pepper planted by applying the stock solution of the liquid fertilizer is better than that of a control group, wherein the growth situation is best when the liquid fertilizer is applied and diluted by 5 times, the plant height is also highest, and the growth situation is good when the experimental group is also grown by 10 times and 20 times when the liquid fertilizer is applied and diluted by 20 times compared with that of the control group, so that the effect of promoting the growth of the pepper by using the liquid fertilizer is demonstrated.
The foregoing is only illustrative of the present invention. The scope of the present invention is not limited thereto, and any changes or simple substitutions which are not thought of through creative efforts should be covered within the scope of the present invention.
SEQUENCE LISTING
<110> Guangxi national university
<120> a liquid fertilizer
<130>2019
<160>1
<170>PatentIn version 3.3
<210>1
<211>1409
<212>DNA
<213> Bacteroides (Bacillus paramycoides)
<400>1
tgcagtcgag cgaatggatt aagagcttgc tcttatgaag ttagcggcgg acgggtgagt 60
aacacgtggg taacctgccc ataagactgg gataactccg ggaaaccggg gctaataccg 120
gataacattt tgaaccgcatggttcgaaat tgaaaggcgg cttcggctgt cacttatgga 180
tggacccgcg tcgcattagc tagttggtga ggtaacggct caccaaggca acgatgcgta 240
gccgacctga gagggtgatc ggccacactg ggactgagac acggcccaga ctcctacggg 300
aggcagcagt agggaatctt ccgcaatgga cgaaagtctg acggagcaac gccgcgtgag 360
tgatgaaggc tttcgggtcg taaaactctg ttgttaggga agaacaagtg ctagttgaat 420
aagctggcac cttgacggta cctaaccaga aagccacggc taactacgtg ccagcagccg 480
cggtaatacg taggtggcaa gcgttatccg gaattattgg gcgtaaagcg cgcgcaggtg 540
gtttcttaag tctgatgtga aagcccacgg ctcaaccgtg gagggtcatt ggaaactggg 600
agacttgagt gcagaagagg aaagtggaat tccatgtgta gcggtgaaat gcgtagagat 660
atggaggaac accagtggcg aaggcgactt tctggtctgt aactgacact gaggcgcgaa 720
agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgagtgct 780
aagtgttaga gggtttccgc cctttagtgc tgaagttaac gcattaagca ctccgcctgg 840
ggagtacggc cgcaaggctg aaactcaaag gaattgacgg gggcccgcac aagcggtgga 900
gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca tcctctgaca 960
accctagaga tagggcttct ccttcgggag cagagtgaca ggtggtgcat ggttgtcgtc 1020
agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt gatcttagtt 1080
gccatcatta agttgggcac tctaaggtga ctgccggtga caaaccggag gaaggtgggg 1140
atgacgtcaa atcatcatgc cccttatgac ctgggctaca cacgtgctac aatggacggt 1200
acaaagagct gcaagaccgc gaggtggagc taatctcata aaaccgttct cagttcggat 1260
tgtaggctgc aactcgccta catgaagctg gaatcgctag taatcgcgga tcagcatgcc 1320
gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgag agtttgtaac 1380
acccgaagtc ggtggggtaa ccttttgga 1409
Claims (9)
1. A liquid fertilizer is characterized by comprising the following steps:
(a) making fermentation base material
Adding feather into culture medium according to a certain proportion, and sterilizing to obtain fermentation substrate;
(b) inoculating strain capable of degrading feather
Inoculating the obtained fermentation substrate with a strain with a certain content capable of degrading feather, wherein the nucleotide sequence of the strain is shown as SEQ ID NO.1, and the preservation number of the strain is GDMCC No: 60687, which is preserved by Guangdong province microorganism strain preservation center, wherein the preservation date is 2019, 6 and 13 months, the preservation address is No. 59, 5 th floor of Mirabilitum 100, Guangzhou city, and the classification category is Bacillussparamycodes;
(c) fermenting the mixture
Fermenting under the condition suitable for the growth of the strains, and after fermenting for a period of time, completing the fermentation to obtain the fermentation liquor.
2. The liquid fertilizer as claimed in claim 1, wherein the culture medium comprises the following components in percentage by mass: k2HPO 4: KH2PO 4: NaCl: MgSO4 ═ 150: 10: 5: 2: 1-1000:10:0.5:0.5:0.1.
3. The liquid fertilizer as claimed in claim 1, wherein the culture medium is added in a proportion of feather: medium 1: 200-1:5.
4. The liquid fertilizer as claimed in claim 1, wherein said conditions suitable for growth of bacterial species include a controlled temperature, said temperature being in the range of 25 to 45 degrees.
5. The liquid fertilizer as claimed in claim 1, wherein said conditions suitable for growth of bacterial species include controlling the pH of said liquid fertilizer to a range of 5 to 8.
6. The liquid fertilizer according to claim 1, wherein said period of time is 24H to 80H.
7. The liquid fertilizer of claim 1, further comprising a drying step of drying the feathers prior to sterilizing the feathers.
8. The liquid fertilizer as claimed in claim 7, further comprising a pulverization step of pulverizing the dried feathers.
9. The liquid fertilizer according to claim 1, further comprising a filtration step of filtering the fermentation broth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911233283.3A CN110922238A (en) | 2019-12-05 | 2019-12-05 | Liquid fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911233283.3A CN110922238A (en) | 2019-12-05 | 2019-12-05 | Liquid fertilizer |
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| CN110922238A true CN110922238A (en) | 2020-03-27 |
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| CN201911233283.3A Pending CN110922238A (en) | 2019-12-05 | 2019-12-05 | Liquid fertilizer |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110804573A (en) * | 2019-12-05 | 2020-02-18 | 广西民族大学 | Bacilloid fungoides fermentation method |
| CN110819572A (en) * | 2019-12-05 | 2020-02-21 | 广西民族大学 | Bacillusbacteroides |
| CN113897306A (en) * | 2021-09-13 | 2022-01-07 | 广西民族大学 | Compound microbial agent and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US4959311A (en) * | 1988-03-31 | 1990-09-25 | North Carolina State University | Method of degrading keratinaceous material and bacteria useful therefore |
| CN106116765A (en) * | 2016-06-28 | 2016-11-16 | 泰山医学院 | A kind of method utilizing fermentable poultry feather to produce liquid organic fertilizer |
| CN107285815A (en) * | 2017-06-20 | 2017-10-24 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of compound amino acid fertilizer and its production method |
| CN109355228A (en) * | 2018-11-20 | 2019-02-19 | 南京林业大学 | One plant of forest rhizosphere bacteria intends bacillus mycoides JYZ-SD5 and its application |
-
2019
- 2019-12-05 CN CN201911233283.3A patent/CN110922238A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4959311A (en) * | 1988-03-31 | 1990-09-25 | North Carolina State University | Method of degrading keratinaceous material and bacteria useful therefore |
| CN106116765A (en) * | 2016-06-28 | 2016-11-16 | 泰山医学院 | A kind of method utilizing fermentable poultry feather to produce liquid organic fertilizer |
| CN107285815A (en) * | 2017-06-20 | 2017-10-24 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of compound amino acid fertilizer and its production method |
| CN109355228A (en) * | 2018-11-20 | 2019-02-19 | 南京林业大学 | One plant of forest rhizosphere bacteria intends bacillus mycoides JYZ-SD5 and its application |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110804573A (en) * | 2019-12-05 | 2020-02-18 | 广西民族大学 | Bacilloid fungoides fermentation method |
| CN110819572A (en) * | 2019-12-05 | 2020-02-21 | 广西民族大学 | Bacillusbacteroides |
| CN110804573B (en) * | 2019-12-05 | 2022-10-21 | 广西民族大学 | Bacilloid fungoides fermentation method |
| CN110819572B (en) * | 2019-12-05 | 2022-10-21 | 广西民族大学 | Bacillusmycoides |
| CN113897306A (en) * | 2021-09-13 | 2022-01-07 | 广西民族大学 | Compound microbial agent and application thereof |
| CN113897306B (en) * | 2021-09-13 | 2023-09-29 | 广西绿友农生物科技股份有限公司 | Composite microbial agent and application thereof |
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