CN110800693A - Production method of antibiotic-free eggs - Google Patents
Production method of antibiotic-free eggs Download PDFInfo
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- CN110800693A CN110800693A CN201911297364.XA CN201911297364A CN110800693A CN 110800693 A CN110800693 A CN 110800693A CN 201911297364 A CN201911297364 A CN 201911297364A CN 110800693 A CN110800693 A CN 110800693A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/195—Antibiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
Abstract
Aiming at the defects of the prior art that Chinese herbal medicines are used for feeding laying hens so as to realize non-antibiotic breeding, the invention provides a production method of non-antibiotic eggs, which is characterized in that active ingredients in crop raw materials are extracted and the Chinese herbal medicines are jointly applied, the active ingredients are doubly added in feeding and drinking water, and the physiological characteristics of the laying hens at different stages are timely adjusted, so that the immunity of the laying hens can be effectively improved, and the real non-antibiotic egg production is realized.
Description
Technical Field
The invention belongs to the technical field of egg production, and particularly relates to a production method of a nonreactive egg.
Background
In recent years, the laying hen breeding technology in China is continuously developed, the egg yield of laying hens is gradually improved, and the benefit of laying hen farmers is further improved. However, the mode of cage-rearing in a shed is commonly adopted in the laying hen cultivation in China, the whole growth stage of the laying hen is always in the limited environment, and the cage-rearing mode is often poor in environmental sanitation, single in feed variety, extensive in overall cultivation management and the like, so that the incidence of diseases and the fatality rate of the laying hen are high, and therefore, in order to avoid the incidence of diseases of the laying hen, a farmer often feeds antibiotics such as penicillin, oxytetracycline, aureomycin and the like to the laying hen, the dosage of the antibiotics is large, the residual antibiotics in the egg are high, and the health of the public is directly influenced.
The Chinese invention patent CN110338141A discloses an application of a whole antibiotic-free breeding technical scheme in the breeding of laying hens, which comprises the following three aspects in the breeding process of the laying hens: (1) mixing the fermented feed and the laying hen compound feed according to the mass ratio (2.5-4): 100 to prepare a mixed daily ration for the laying hens, and feeding the mixed daily ration for the laying hens in the whole process; (2) mixing fermented Chinese herbal medicines with laying hen daily ration according to the mass ratio (1-5): 1000, preparing the health-care daily ration for the laying hens, and continuously feeding the laying hens for 7-10 days in the middle and last ten days of the month; (3) and (3) spraying and drying the microbial deodorizing bactericide once every 5-7 days. Although the Chinese herbal medicine daily ration can improve the survival rate, the laying rate and the egg quality of laying hens, the weight of rejected chickens and prolong the egg laying peak period to a certain extent, the adopted Chinese herbal medicine daily ration can treat diseases when the laying hens get ill, but cannot improve the immunity of the laying hens fundamentally and cannot realize nonreactive feeding really.
Disclosure of Invention
Aiming at the defects of the prior art that Chinese herbal medicines are used for feeding laying hens so as to realize non-antibiotic breeding, the invention provides a production method of non-antibiotic eggs, which is characterized in that active ingredients in crop raw materials are extracted and the Chinese herbal medicines are jointly applied, the active ingredients are doubly added in feeding and drinking water, and the physiological characteristics of the laying hens at different stages are timely adjusted, so that the immunity of the laying hens can be effectively improved, and the real non-antibiotic egg production is realized.
In order to realize the purpose, the invention adopts the following technical scheme:
a production method of non-resistant eggs comprises the following steps:
(1) brood time 0-42 days old: conventional vaccination was performed as required, while feeding and drinking were performed as follows:
feeding: feeding the young egg chickens at the age of 0-30 days, feeding the young egg chickens at the age of 30-42 days, uniformly mixing the feed, the health care daily ration and the cactus pulp in a weight ratio of 3:1:1, continuously feeding for 3-5 days, and uniformly mixing the feed and the health care daily ration in a weight ratio of 3:1 in the rest time, and feeding;
drinking water: diluting the health water by 50-70 times, and using as daily drinking water;
the vaccination is carried out according to the conventional technical means in the field, for example, according to the following procedures, 7 days old: inoculating new, branch and method freeze-dried seedlings, and injecting small triple oil seedlings with dosage and muscle; the age of 10 days: the bursa of Fabricius freeze-dried seedlings are dripped into the nose, dropped into eyes or drunk; age of 14 days: intramuscular injection of avian influenza, the serotype of the vaccine strain should match the serotype of the epidemic; age of 17 days: the bursa of Fabricius seedling is dripped into the nose, dropped into the eyes or drunk water.
(2) Incubation period of 43-97 days old: feeding and drinking water were carried out as follows:
feeding: uniformly mixing the laying hen feed and the health-care daily ration in a weight ratio of 3:1, and feeding;
drinking water: diluting the health water by 30-50 times, and using as daily drinking water;
(3) edd of 98-130 days old: feeding and drinking water were carried out as follows:
feeding: uniformly mixing the pre-laying chicken feed, the health-care daily ration and the cactus pulp in a weight ratio of 3:1:1, and feeding;
drinking water: diluting the health water by 30-50 times, and using as daily drinking water;
(4) and (3) an egg producing period:
initial stage of 131-:
feeding: uniformly mixing the chicken feed in the initial stage and the health-care daily ration in a weight ratio of 3:1, feeding, uniformly mixing the chicken feed in the initial stage and the health-care daily ration in a weight ratio of 2:1 every 15-20 days, and continuously feeding for 3-5 days;
drinking water: diluting the health water by 30-50 times, and using as daily drinking water;
176-315 days old high-yield period:
feeding: uniformly mixing the high-yield chicken feed and the health-care daily ration in a weight ratio of 3:1, and feeding;
drinking water: diluting the health water by 20-30 times, and using as daily drinking water;
end-term at age of 316-:
feeding: feeding with chicken feed at the later stage of high yield;
drinking water: diluting the health water by 20-30 times, and using as daily drinking water;
the health-care daily ration in the steps (1) to (4) is prepared by adopting the following method:
a. uniformly mixing crushed crop straw raw materials (the crop straws can be corn straws, wheat straws and the like) with a sodium bicarbonate solution with the mass concentration of 1.5-3% according to the weight ratio of 1:2-3 (acid-base imbalance can cause a series of diseases, sodium bicarbonate is used as an acid-base balance regulator, and the sodium bicarbonate can prevent organism abnormality or a sub-health state is usually added), placing the mixture into a blasting machine for blasting, uniformly mixing the blasting material obtained by blasting with the sodium bicarbonate solution with the mass concentration of 1.5-3% according to the weight ratio of 1:2-3, treating the mixture for 20-30min at the temperature of 150-170 ℃ under an anaerobic condition, then cooling to 120 ℃ under 100-temperature, and continuously treating for 1.5-3h, and performing solid-liquid separation to obtain a first-treated solid phase and a first-treated liquid phase;
b. uniformly mixing the primary treated solid phase obtained in the step a with the crushed traditional Chinese medicine raw materials to obtain a fermentation substrate, uniformly mixing the fermentation substrate with an inorganic salt solution according to the weight ratio of 1:5-7, adding probiotic components, culturing for 3-5 days at the temperature of 30-40 ℃, and then carrying out solid-liquid separation to obtain a secondary treated solid phase and a secondary treated liquid phase;
c. b, using the secondary treated solid phase obtained in the step b as health-care daily ration for feeding laying hens, combining the primary treated liquid phase obtained in the step a and the secondary treated liquid phase obtained in the step b to be used as health-care water, and diluting the health-care water to replace drinking water of the laying hens;
wherein the weight ratio of the crop straw raw materials to the traditional Chinese medicine raw materials is as follows: 0.3 to 0.5 portion of honeysuckle, 0.5 to 0.7 portion of isatis root, 1.2 to 2.0 portions of dandelion, 0.3 to 0.5 portion of bupleurum and 50 to 80 portions of crop straw.
Preferably, in step a, the specific operation of blasting is as follows: after the pressure is maintained for 100 seconds and 200 seconds under the condition that the pressure is 1.5-2.5 MPa, the pressure is released instantly.
Preferably, in step b, the inorganic salt solution is prepared from (NH)4)2SO41.2g、FeSO47.0mg、MnSO41.7mg、KH2PO42.3g、CaCl20.3g、NaCl 0.6g、ZnCl21.5mg and 1000mL of distilled water were mixed uniformly and then adjusted to neutral with a sodium bicarbonate solution.
Preferably, in the step b, the probiotic component is prepared by mixing bacterial liquids of lactobacillus casei, enterococcus faecalis, bacillus subtilis and saccharomyces boulardii in a volume ratio of 1:1:1:1, and the effective viable count of the bacterial liquids of the lactobacillus casei, the enterococcus faecalis, the bacillus subtilis and the saccharomyces boulardii is 1.0 × 10 cfu/mL.
Preferably, glucose and vitamin complex are added into the drinking water in the brooding period in the step (1), wherein the adding amount of the glucose is 5-7% by weight, and the adding amount of the vitamin complex is 3-5% by weight.
Preferably, the multivitamins comprise the following components: 10-15 parts of vitamin A, 10-30 parts of vitamin C and 7-12 parts of vitamin D.
Preferably, vitamin C is added into the drinking water in the expected delivery period in the step (3), and the addition amount of the vitamin C is 3-5% by weight.
Preferably, vitamin C is added into the drinking water in the initial stage in the step (4), and the addition amount of the vitamin C is 3-5% by weight.
Vitamin C participates in amino acid metabolism, synthesis of neurotransmitter, and synthesis of collagen and intercellular substance, and has effects of reducing capillary permeability, stimulating blood coagulation function, accelerating blood coagulation, promoting absorption of iron in intestinal tract, and increasing resistance to infection. Has effects in removing toxic substances, and has antihistaminic effect, and can be used for the adjuvant treatment of hemopoiesis stimulating, toxic substance removing, summer heat relieving, and hyperpyrexia.
Preferably, the compound amino acid is added into the drinking water with high yield in the step (4), and the adding amount of the compound amino acid is 5-10% by weight.
Preferably, the compound amino acid comprises the following components in parts by weight: 3-7 parts of glycine, 3-5 parts of alanine, 1-3 parts of valine, 4-7 parts of leucine, 2-6 parts of aspartic acid and 3-8 parts of methionine.
The invention has the following beneficial effects:
for laying hens in different growth stages, feeds in different mature stages on the market can be used and are divided into chick feeds, young chick feeds, pre-birth feeds, early-birth feeds, peak feeds and high-yield later feeds according to the growth stages. In order to ensure the most basic nutritional requirements of the growth of the laying hens, the feed of the laying hens in the corresponding growth stage is used as the staple food for feeding, and in order to realize the growth of the non-resistant eggs, the immunity of the chicken flocks needs to be improved fundamentally, and the antibiotics are used for preventing the chicken flocks from being ill. Therefore, the health-care daily ration is added on the basis of the main ration, and the drinking water is replaced by the health-care water, wherein the health-care daily ration is obtained by performing two-stage heat treatment on the blasted crop raw materials and then mixing Chinese herbal medicines for fermentation; and a part of health-care water containing the active ingredients is formed after two-stage heat treatment, then the health-care water is fermented by mixing Chinese herbal medicines to obtain health-care daily ration containing the Chinese herbal medicine ingredients and fermented water, and the fermented water is mixed with the health-care water to obtain the final health-care water. According to the preparation process of the health-care daily ration and the health-care water, the active ingredients contained in the health-care daily ration and the health-care water can improve the immunity of the laying hens and treat diseases when the laying hens are ill, so that the production of non-antibiotic eggs is truly realized.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to specific examples.
Example 1
In this embodiment, the health-care daily ration is prepared by the following method:
d. uniformly mixing the crushed crop straw raw material with a sodium bicarbonate solution with the mass concentration of 1.5% according to the weight ratio of 1:2, placing the mixture in a blasting machine for blasting, uniformly mixing the blasting material obtained by blasting with the sodium bicarbonate solution with the mass concentration of 1.5% according to the weight ratio of 1:2, treating the mixture for 30min at the temperature of 170 ℃ under an anaerobic condition, cooling the mixture to 120 ℃, continuously treating the mixture for 3h, and performing solid-liquid separation to obtain a first-treatment solid phase and a first-treatment liquid phase;
e. uniformly mixing the primary treated solid phase obtained in the step a with the crushed traditional Chinese medicine raw materials to obtain a fermentation substrate, uniformly mixing the fermentation substrate with an inorganic salt solution according to the weight ratio of 1:5, adding probiotic components, culturing for 5 days at the temperature of 30 ℃, and then carrying out solid-liquid separation to obtain a secondary treated solid phase and a secondary treated liquid phase;
f. b, using the secondary treated solid phase obtained in the step b as health-care daily ration for feeding laying hens, combining the primary treated liquid phase obtained in the step a and the secondary treated liquid phase obtained in the step b to be used as health-care water, and diluting the health-care water to replace drinking water of the laying hens;
wherein the weight ratio of the crop straw raw materials to the traditional Chinese medicine raw materials is as follows: 0.3 part of honeysuckle, 0.5 part of isatis root, 1.2 parts of dandelion, 0.3 part of radix bupleuri and 50 parts of crop straw.
In this embodiment, in step a, the blasting specifically includes: after keeping the pressure for 200s under the condition of 1.7MPa, the pressure is released instantly.
In this example, the inorganic salt solution was prepared from (NH) in step b4)2SO41.2g、FeSO47.0mg、MnSO41.7mg、KH2PO42.3g、CaCl20.3g、NaCl 0.6g、ZnCl21.5mg and 1000mL of distilled water were mixed uniformly and then adjusted to neutral with a sodium bicarbonate solution.
In this embodiment, in the step b, the probiotic component is prepared by mixing lactobacillus casei, enterococcus faecalis, bacillus subtilis and saccharomyces boulardii bacteria liquid in a volume ratio of 1:1:1:1, and the effective viable count of the lactobacillus casei, enterococcus faecalis, bacillus subtilis and saccharomyces boulardii bacteria liquid is 1.0 × 10 cfu/mL.
A production method of non-resistant eggs comprises the following steps:
the daily age division of the following stages is only used for reference, the chickens can be flexibly adjusted into groups according to the growth difference of different chickens in actual breeding, and the feed used in the daily ration of the following stages is purchased from conventional feed on the market;
(1) brood time 0-42 days old: conventional vaccination was performed as required, while feeding and drinking were performed as follows:
feeding: feeding the young egg chickens at the age of 0-30 days, feeding the young egg chickens at the age of 30-42 days, uniformly mixing the feed, the health care daily ration and the cactus pulp in a weight ratio of 3:1:1, continuously feeding for 3-5 days, and uniformly mixing the feed and the health care daily ration in a weight ratio of 3:1 in the rest time, and feeding; the immunity of the young laying hens is low, the young laying hens are easy to get ill and get ill in a large range, and the applicant finds that the incidence rate of large-scale ill diseases can be obviously reduced by feeding the cactus pulp in the later brooding period through years of practice, so that the daily ration mixed with part of the cactus pulp is adopted in the later brooding period; the cactus in the cactus food is selected from edible cactus types;
drinking water: in consideration of the delicate intestine and stomach of the laying chicks, the health-care water is diluted by 50-70 times and then used as daily drinking water;
the vaccination is carried out according to the conventional technical means in the field, for example, according to the following procedures, 7 days old: inoculating new, branch and method freeze-dried seedlings, and injecting small triple oil seedlings with dosage and muscle; the age of 10 days: the bursa of Fabricius freeze-dried seedlings are dripped into the nose, dropped into eyes or drunk; age of 14 days: intramuscular injection of avian influenza, the serotype of the vaccine strain should match the serotype of the epidemic; age of 17 days: the bursa of Fabricius seedling is dripped into the nose, dropped into the eyes or drunk water.
(2) Incubation period of 43-97 days old: feeding and drinking water were carried out as follows:
feeding: uniformly mixing the laying hen feed and the health-care daily ration in a weight ratio of 3:1, and feeding; the resistance of the chickens in the breeding period is stronger, so the consumption of the health care daily ration is slightly lower, and the cactus pulp is not needed to be eaten;
drinking water: diluting the health water by 30-50 times, and using as daily drinking water;
(3) edd of 98-130 days old: feeding and drinking water were carried out as follows:
feeding: uniformly mixing the pre-laying chicken feed, the health-care daily ration and the cactus pulp in a weight ratio of 3:1:1, and feeding;
drinking water: diluting the health water by 30-50 times, and using as daily drinking water;
the laying hen body maturation and sexual maturation tasks are carried on the shoulder in the predicted laying period, and special attention needs to be paid to the reasonable supply of nutrition, so that the laying stress is reduced to the maximum extent, the early laying fatigue syndrome is prevented, and the foundation is laid for entering the laying peak period as soon as possible. The estimated delivery period is the key period of sexual maturity of the laying hens. After the laying hens are 14 weeks old, the volume and the weight of an ovary and an oviduct begin to increase rapidly, the growth rate is faster after 17 weeks old, and the reproductive system of most of the 19 weeks old chickens grows to be nearly mature. The rapid development of the reproductive system at this stage can affect the feeding and emotion of the chicken to a certain extent, and has adverse effect on the resistance, so that cactus pulp also needs to be fed at this stage;
initial stage of 131-:
feeding: uniformly mixing the chicken feed in the initial stage and the health-care daily ration in a weight ratio of 3:1, feeding, uniformly mixing the chicken feed in the initial stage and the health-care daily ration in a weight ratio of 2:1 every 15-20 days, and continuously feeding for 3-5 days;
drinking water: diluting the health water by 30-50 times, and using as daily drinking water;
the initial stage of labor: the laying hens are suddenly shifted from a non-laying state to a laying state, hormone secretion in bodies is unstable, abnormal eggs, eggs with blood and the like are frequently generated due to resistance reduction, and even the eggs die suddenly, so that the health-care daily ration is continuously fed for 3-5 days at half a month interval, and the occurrence of oviduct inflammation of the laying hens is avoided.
176-315 days old high-yield period:
feeding: uniformly mixing the high-yield chicken feed and the health-care daily ration in a weight ratio of 3:1, and feeding;
drinking water: diluting the health water by 20-30 times, and using as daily drinking water;
high-yield period: the method is the period with the highest benefit for feeding the laying hens, and the feeding in the period mainly promotes high yield, prolongs high yield period and reduces death and elimination number, so that compared with the initial stage, the proportion of health care daily ration is reduced, the nutritional requirement of the laying hens at the moment is ensured, and the egg yield is prevented from being influenced.
End-term at age of 316-:
feeding: feeding with chicken feed at the later stage of high yield;
drinking water: diluting the health water by 20-30 times, and using as daily drinking water;
final delivery period: at the moment, the laying rate is reduced to be below 80 percent, the laying performance of laying hens is reduced, the quality of eggshells is easy to deteriorate, at the moment, the laying hens are preferably fed with the feed only in the later period of high yield, health-care daily ration is not added, and the concentration of health-care water is properly increased when the laying hens drink water to avoid diseases.
In order to further ensure the good growth of the chicks, in this embodiment, glucose and multivitamins are added to the drinking water during the brooding period in the step (1), wherein the adding amount of the glucose is 5% by weight, and the adding amount of the multivitamins is 5% by weight; the compound vitamin comprises the following components: 10 parts of vitamin A, 30 parts of vitamin C and 7 parts of vitamin D.
In order to enhance the immunity of the chicken in the expected delivery period, in this embodiment, vitamin C is added to the drinking water in the expected delivery period in the step (3), and the addition amount of the vitamin C is 5% by weight.
Similarly, vitamin C is added into the drinking water in the initial stage in the step (4), and the addition amount of the vitamin C is 3-5% by weight.
For chickens with high delivery period, the demand for protein is high, so in this embodiment, the compound amino acid is added into the drinking water with high delivery period in the step (4), and the adding amount of the compound amino acid is 10% by weight; the compound amino acid comprises the following components in parts by weight: 7 parts of glycine, 5 parts of alanine, 1 part of valine, 5 parts of leucine, 4 parts of aspartic acid and 5 parts of methionine; in the form of compound amino acid, the chicken only takes in drinking water, which is more beneficial to the absorption in the body.
Example 2
Example 2 differs from example 1 in that:
the preparation of the health-care daily ration in the embodiment is specifically prepared by the following method:
a. uniformly mixing the crushed crop straw raw material with a sodium bicarbonate solution with the mass concentration of 2% according to the weight ratio of 1:2, placing the mixture in a blasting machine for blasting, uniformly mixing the blasting material obtained by blasting with the sodium bicarbonate solution with the mass concentration of 2% according to the weight ratio of 1:3, treating the mixture for 25min at the temperature of 160 ℃ under anaerobic conditions, cooling the mixture to 110 ℃, continuously treating the mixture for 2h, and performing solid-liquid separation to obtain a first treatment solid phase and a first treatment liquid phase;
b. uniformly mixing the primary treated solid phase obtained in the step a with the crushed traditional Chinese medicine raw materials to obtain a fermentation substrate, uniformly mixing the fermentation substrate with an inorganic salt solution according to the weight ratio of 1:5, adding probiotic components, culturing for 4 days at the temperature of 30 ℃, and then carrying out solid-liquid separation to obtain a secondary treated solid phase and a secondary treated liquid phase;
c. the secondary treatment solid phase obtained in the step b is used as health-care daily ration for feeding laying hens, and the primary treatment liquid phase obtained in the step a and the step
b, merging the secondary treatment liquid phases to be used as health water to be diluted to replace drinking water of the laying hens;
wherein the weight ratio of the crop straw raw materials to the traditional Chinese medicine raw materials is as follows: 0.4 part of honeysuckle, 0.7 part of isatis root, 1.5 parts of dandelion, 0.3 part of radix bupleuri and 60 parts of crop straw.
In this embodiment, in step a, the blasting specifically includes: after keeping the pressure for 170s under the condition of 1.8MPa, the pressure is released instantly.
In this embodiment, in step b, theThe inorganic salt solution is prepared from (NH)4)2SO41.2g、FeSO47.0mg、MnSO41.7mg、KH2PO42.3g、CaCl20.3g、NaCl 0.6g、ZnCl21.5mg and 1000mL of distilled water were mixed uniformly and then adjusted to neutral with a sodium bicarbonate solution.
In this embodiment, in the step b, the probiotic component is prepared by mixing lactobacillus casei, enterococcus faecalis, bacillus subtilis and saccharomyces boulardii bacteria liquid in a volume ratio of 1:1:1:1, and the effective viable count of the lactobacillus casei, enterococcus faecalis, bacillus subtilis and saccharomyces boulardii bacteria liquid is 1.0 × 10 cfu/mL.
Example 3
Example 3 differs from example 1 in that:
in this embodiment, the health-care daily ration is prepared by the following method:
a. uniformly mixing the crushed crop straw raw material with a sodium bicarbonate solution with the mass concentration of 3% according to the weight ratio of 1:3, placing the mixture in a blasting machine for blasting, uniformly mixing the blasting material obtained by blasting with the sodium bicarbonate solution with the mass concentration of 3% according to the weight ratio of 1:2, treating the mixture at the temperature of 150 ℃ for 20min under anaerobic conditions, cooling the mixture to 100 ℃, continuously treating the mixture for 1.5h, and performing solid-liquid separation to obtain a first treatment solid phase and a first treatment liquid phase;
b. uniformly mixing the primary treated solid phase obtained in the step a with the crushed traditional Chinese medicine raw materials to obtain a fermentation substrate, uniformly mixing the fermentation substrate with an inorganic salt solution according to the weight ratio of 1:7, adding probiotic components, culturing for 3 days at the temperature of 40 ℃, and then carrying out solid-liquid separation to obtain a secondary treated solid phase and a secondary treated liquid phase;
c. the secondary treatment solid phase obtained in the step b is used as health-care daily ration for feeding laying hens, and the primary treatment liquid phase obtained in the step a and the step
b, merging the secondary treatment liquid phases to be used as health water to be diluted to replace drinking water of the laying hens;
wherein the weight ratio of the crop straw raw materials to the traditional Chinese medicine raw materials is as follows: 0.5 part of honeysuckle, 0.7 part of isatis root, 2.0 parts of dandelion, 0.3 part of radix bupleuri and 80 parts of crop straw.
In this embodiment, in step a, the blasting specifically includes: and (4) maintaining the pressure for 120s under the condition of 2.5MPa, and then releasing the pressure instantly.
In this example, the inorganic salt solution was prepared from (NH) in step b4)2SO41.2g、FeSO47.0mg、MnSO41.7mg、KH2PO42.3g、CaCl20.3g、NaCl 0.6g、ZnCl21.5mg and 1000mL of distilled water were mixed uniformly and then adjusted to neutral with a sodium bicarbonate solution.
In this embodiment, in the step b, the probiotic component is prepared by mixing lactobacillus casei, enterococcus faecalis, bacillus subtilis and saccharomyces boulardii bacteria liquid in a volume ratio of 1:1:1:1, and the effective viable count of the lactobacillus casei, enterococcus faecalis, bacillus subtilis and saccharomyces boulardii bacteria liquid is 1.0 × 10 cfu/mL.
Example 4
Example 4 differs from example 1 in that:
in this embodiment, the health-care daily ration is prepared by the following method:
a. uniformly mixing the crushed crop straw raw material with a sodium bicarbonate solution with the mass concentration of 2% according to the weight ratio of 1:2.5, placing the mixture in a blasting machine for blasting, uniformly mixing the blasting material obtained by blasting with the sodium bicarbonate solution with the mass concentration of 2% according to the weight ratio of 1:2.5, treating the mixture for 25min at the temperature of 160 ℃ under an anaerobic condition, cooling the mixture to 120 ℃, continuously treating the mixture for 3h, and performing solid-liquid separation to obtain a first-treatment solid phase and a first-treatment liquid phase;
b. uniformly mixing the primary treated solid phase obtained in the step a with the crushed traditional Chinese medicine raw materials to obtain a fermentation substrate, uniformly mixing the fermentation substrate with an inorganic salt solution according to the weight ratio of 1:7, adding probiotic components, culturing for 5 days at the temperature of 35 ℃, and then carrying out solid-liquid separation to obtain a secondary treated solid phase and a secondary treated liquid phase;
c. the secondary treatment solid phase obtained in the step b is used as health-care daily ration for feeding laying hens, and the primary treatment liquid phase obtained in the step a and the step
b, merging the secondary treatment liquid phases to be used as health water to be diluted to replace drinking water of the laying hens;
wherein the weight ratio of the crop straw raw materials to the traditional Chinese medicine raw materials is as follows: 0.5 part of honeysuckle, 0.6 part of isatis root, 1.8 parts of dandelion, 0.5 part of radix bupleuri and 70 parts of crop straw.
In this embodiment, in step a, the blasting specifically includes: and (3) maintaining the pressure for 180s under the condition that the pressure is 2.2MPa, and then releasing the pressure instantly.
In this example, the inorganic salt solution was prepared from (NH) in step b4)2SO41.2g、FeSO47.0mg、MnSO41.7mg、KH2PO42.3g、CaCl20.3g、NaCl 0.6g、ZnCl21.5mg and 1000mL of distilled water were mixed uniformly and then adjusted to neutral with a sodium bicarbonate solution.
In this embodiment, in the step b, the probiotic component is prepared by mixing lactobacillus casei, enterococcus faecalis, bacillus subtilis and saccharomyces boulardii bacteria liquid in a volume ratio of 1:1:1:1, and the effective viable count of the lactobacillus casei, enterococcus faecalis, bacillus subtilis and saccharomyces boulardii bacteria liquid is 1.0 × 10 cfu/mL.
Laying hen feeding test
500 laying hens were selected for the test and randomly divided into 5 groups including 1 blank control group (feeding basal diet and regular drinking water) and 4 test groups, and the laying hens were fed in the test groups 1-4 according to the methods provided in examples 1-4, respectively.
TABLE 1 growth index parameters of layers of examples 1-4 and control
As can be seen from table 1, compared with the control group, examples 1 to 4 of the present invention have almost the same feed-egg ratio, but can significantly reduce the mortality rate of chicken flocks, improve the laying rate of chicken flocks, and increase the yield of eggs, so the present invention not only avoids the use of antibiotics, but also achieves the reduction of mortality rate and the improvement of laying rate, and fundamentally solves the problem that the residue of antibiotics in eggs is harmful to human health, and satisfies the demand of modern people for food health.
Finally, it should be noted that: the above embodiments are merely illustrative and not restrictive of the technical solutions of the present invention, and any equivalent substitutions and modifications or partial substitutions made without departing from the spirit and scope of the present invention should be included in the scope of the claims of the present invention.
Claims (10)
1. A production method of a nonreactive egg is characterized by comprising the following steps:
(1) and (3) in the brooding period: conventional vaccination was performed as required, while feeding and drinking were performed as follows:
feeding: feeding the young egg chickens at the age of 0-30 days, feeding the young egg chickens at the age of 30-42 days, uniformly mixing the feed, the health care daily ration and the cactus pulp in a weight ratio of 3:1:1, continuously feeding for 3-5 days, and uniformly mixing the feed and the health care daily ration in a weight ratio of 3:1 in the rest time, and feeding;
drinking water: diluting the health water by 50-70 times, and using as daily drinking water;
(2) and (3) during the growing period: feeding and drinking water were carried out as follows:
feeding: uniformly mixing the laying hen feed and the health-care daily ration in a weight ratio of 3:1, and feeding;
drinking water: diluting the health water by 30-50 times, and using as daily drinking water;
(3) the predicted delivery period: feeding and drinking water were carried out as follows:
feeding: uniformly mixing the pre-laying chicken feed, the health-care daily ration and the cactus pulp in a weight ratio of 3:1:1, and feeding;
drinking water: diluting the health water by 30-50 times, and using as daily drinking water;
(4) and (3) an egg producing period:
the initial stage of labor:
feeding: uniformly mixing the chicken feed in the initial stage and the health-care daily ration in a weight ratio of 3:1, feeding, uniformly mixing the chicken feed in the initial stage and the health-care daily ration in a weight ratio of 2:1 every 15-20 days, and continuously feeding for 3-5 days;
drinking water: diluting the health water by 30-50 times, and using as daily drinking water;
high-yield period:
feeding: uniformly mixing the high-yield chicken feed and the health-care daily ration in a weight ratio of 3:1, and feeding;
drinking water: diluting the health water by 20-30 times, and using as daily drinking water;
final delivery period:
feeding: feeding with chicken feed at the later stage of high yield;
drinking water: diluting the health water by 20-30 times, and using as daily drinking water;
the health-care daily ration in the steps (1) to (4) is prepared by adopting the following method:
a. uniformly mixing the crushed crop straw raw material with a sodium bicarbonate solution with the mass concentration of 1.5-3% according to the weight ratio of 1:2-3, placing the mixture in a blasting machine for blasting, uniformly mixing the blasting material obtained by blasting with the sodium bicarbonate solution with the mass concentration of 1.5-3% according to the weight ratio of 1:2-3, treating the mixture for 20-30min at the temperature of 170 ℃ under an anaerobic condition, cooling to 120 ℃ under the temperature of 100 ℃, continuously treating for 1.5-3h, and performing solid-liquid separation to obtain a first-treated solid phase and a first-treated liquid phase;
b. uniformly mixing the primary treated solid phase obtained in the step a with the crushed traditional Chinese medicine raw materials to obtain a fermentation substrate, uniformly mixing the fermentation substrate with an inorganic salt solution according to the weight ratio of 1:5-7, adding probiotic components, culturing for 3-5 days at the temperature of 30-40 ℃, and then carrying out solid-liquid separation to obtain a secondary treated solid phase and a secondary treated liquid phase;
c. b, using the secondary treated solid phase obtained in the step b as health-care daily ration for feeding laying hens, combining the primary treated liquid phase obtained in the step a and the secondary treated liquid phase obtained in the step b to be used as health-care water, and diluting the health-care water to replace drinking water of the laying hens;
wherein the weight ratio of the crop straw raw materials to the traditional Chinese medicine raw materials is as follows: 0.3 to 0.5 portion of honeysuckle, 0.5 to 0.7 portion of isatis root, 1.2 to 2.0 portions of dandelion, 0.3 to 0.5 portion of bupleurum and 50 to 80 portions of crop straw.
2. The method for producing eggs without resistance according to claim 1, wherein in the step a, the specific operation of blasting is as follows: after the pressure is maintained for 100 seconds and 200 seconds under the condition that the pressure is 1.5-2.5 MPa, the pressure is released instantly.
3. The method for producing eggs free of antibiotics according to claim 1, wherein the inorganic salt solution is prepared from (NH) in step b4)2SO41.2g、FeSO47.0mg、MnSO41.7mg、KH2PO42.3g、CaCl20.3g、NaCl 0.6g、ZnCl21.5mg and 1000mL of distilled water were mixed uniformly and then adjusted to neutral with a sodium bicarbonate solution.
4. The method for producing the antibiotic-free egg according to the claim 1, wherein in the step b, the probiotic components are prepared by mixing bacterial solutions of lactobacillus casei, enterococcus faecalis, bacillus subtilis and saccharomyces boulardii in a volume ratio of 1:1:1:1, and the effective viable count of the bacterial solutions of the lactobacillus casei, the enterococcus faecalis, the bacillus subtilis and the saccharomyces boulardii is 1.0 x 10 cfu/mL.
5. The method for producing non-resistant eggs according to claim 1, wherein glucose and vitamin complex are added to the drinking water during the brooding period in step (1), wherein the amount of glucose added is 5-7% by weight, and the amount of vitamin complex added is 3-5% by weight.
6. The method for producing a nonreactive egg according to claim 5, wherein the vitamin complex comprises the following components: 10-15 parts of vitamin A, 10-30 parts of vitamin C and 7-12 parts of vitamin D.
7. The method for producing a nonreactive egg according to claim 1, wherein vitamin C is added to the drinking water in the expected production period in step (3) in an amount of 3 to 5% by weight.
8. The method for producing non-resistant eggs according to claim 1, wherein vitamin C is added to the drinking water in the initial stage of the production in step (4), and the amount of the added vitamin C is 3-5% by weight.
9. The method for producing eggs without resistance according to claim 1, wherein the compound amino acid is added into the drinking water in high-yield period in the step (4), and the adding amount of the compound amino acid is 5-10% by weight.
10. The production method of the non-resistant eggs according to claim 9, wherein the compound amino acid comprises the following components in parts by weight: 3-7 parts of glycine, 3-5 parts of alanine, 1-3 parts of valine, 4-7 parts of leucine, 2-6 parts of aspartic acid and 3-8 parts of methionine.
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