CN110786329B - Functional preparation for improving strawberry gray mold resistance and preparation method and application thereof - Google Patents
Functional preparation for improving strawberry gray mold resistance and preparation method and application thereof Download PDFInfo
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- CN110786329B CN110786329B CN201911140567.8A CN201911140567A CN110786329B CN 110786329 B CN110786329 B CN 110786329B CN 201911140567 A CN201911140567 A CN 201911140567A CN 110786329 B CN110786329 B CN 110786329B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
Abstract
The invention provides a functional preparation for improving strawberry gray mold resistance and a preparation method and application thereof, belonging to the technical field of strawberry disease control, wherein the functional preparation takes epigallocatechin gallate as an active ingredient; the functional preparation contains 0.5-1.2% of epigallocatechin gallate by weight percent. The functional preparation provided by the invention is convenient to use, low in cost, capable of effectively preventing and treating gray mold of strawberries, and widely applied to strawberry production.
Description
Technical Field
The invention belongs to the technical field of strawberry disease control, and particularly relates to a functional preparation for improving strawberry gray mold resistance, and a preparation method and application thereof.
Background
Gray mold is an important disease in strawberry production, and the disease of greenhouse-cultivated strawberries is particularly serious. Strawberry gray mold mainly damages leaves, flowers and fruits. The quality of the strawberries is seriously influenced when the fruits are attacked, and the production of the strawberries is seriously lost. Since strawberries are generally eaten fresh mainly, the requirements for pesticide residues in strawberry production are very strict. At present, chemical pesticide is mainly used for preventing and treating gray mold of strawberries in production, and due to continuous use for many years, the drug resistance of gray mold germs to medicaments is accelerated, so that the prevention and treatment effect is not ideal, and pesticide residues are easy to generate. Therefore, screening safe functional preparations and application technology thereof to prevent and treat gray mold are important problems in the development of the strawberry industry at present.
Epigallocatechin gallate (EGCG for short) is the main component of tea polyphenols in green tea, and can be separated from tea to obtain catechin monomer. The research widely believes that the biological activity in the tea leaves is mainly caused by the anti-oxidation effect of EGCG. Research in clinical medicine shows that EGCG has the health functions of resisting oxidation, resisting cancer, protecting cell DNA damage, etc. Although the effect of EGCG is well known by relevant researchers at home and abroad in the fields of human and zoology and is widely applied, the research of EGCG in the agricultural field is very little, and no relevant application case is found. In recent years, with the continuous and deep research on EGCG purification technology and equipment, the purification and preparation cost of EGCG is obviously reduced, so that a huge development space is generated for the application of EGCG in the agricultural field.
In addition, because EGCG is easily oxidized in the air and turns into pink, the application effect of the EGCG has certain limitation.
Disclosure of Invention
In view of the above, the present invention aims to provide a functional preparation for improving gray mold resistance of strawberry, and a preparation method and an application thereof; the functional preparation takes EGCG as a main active ingredient, has strong safety, no toxic or side effect, convenient use and low cost.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a functional preparation for improving the gray mold resistance of strawberries, which takes epigallocatechin gallate as an active ingredient; the functional preparation contains 0.5-1.2% of epigallocatechin gallate by weight percent.
Preferably, the functional preparation contains 0.8-1.0% of epigallocatechin gallate in percentage by mass.
Preferably, the functional preparation comprises the following components in 100 mL:
preferably, the functional preparation comprises the following components in 100 mL:
the invention provides a preparation method of the functional preparation, which comprises the following steps:
1) dissolving potassium dihydrogen phosphate and dimethyl hydrogen phosphate trihydrate with water to obtain a salt solution;
2) mixing the epigallocatechin gallate and the ascorbic acid with the salt solution, and diluting to a constant volume to obtain the functional preparation.
The invention provides application of the functional preparation in improving the gray mold resistance of strawberries.
Preferably, the functional preparation is diluted with water and sprayed on the strawberry.
Preferably, the dilution factor is 100-200 times.
Preferably, the dilution factor is 140-160 times.
Preferably, the spraying is carried out once every other day for 3-8 times in total, and the amount of each spraying is that the spraying is carried out until the leaf surfaces are wet and do not drip.
The invention has the beneficial effects that: the functional preparation for improving the gray mold resistance of the strawberry provided by the invention takes epigallocatechin gallate as an active ingredient; the functional preparation contains 0.5-1.2% of epigallocatechin gallate by weight percent. The invention takes the main component of the green tea, namely the epigallocatechin gallate as an active component, is safe, has no toxic or side effect, and has good prevention effect.
Furthermore, the invention takes the monopotassium phosphate and the dipotassium phosphate trihydrate as the auxiliary agents to adjust the pH value of the preparation, and simultaneously takes the ascorbic acid as the auxiliary agent, thereby effectively slowing down the oxidation of the EGCG, increasing the application effect of the EGCG, and ensuring that the EGCG can continuously improve the resistance of the strawberry to the gray mold. The functional preparation provided by the invention is convenient to use, low in cost, capable of effectively preventing and treating gray mold of strawberries, and widely applied to strawberry production.
Detailed Description
The invention provides a functional preparation for improving the gray mold resistance of strawberries, which takes epigallocatechin gallate as an active ingredient; the functional preparation contains 0.5-1.2% of epigallocatechin gallate by weight percent.
In the present invention, the content of epigallocatechin gallate in the functional preparation is preferably 0.8 to 1.0% by mass. The source and specification of epigallocatechin gallate are not particularly limited in the present invention, and commercially available products that are conventional in the art may be used. According to the invention, the epigallocatechin gallate is used as an active ingredient, and the epigallocatechin gallate can improve the resistance of the gray mold of the strawberry and can effectively prevent and treat the gray mold of the strawberry.
In the present invention, the functional preparation is preferably a liquid preparation, and the functional preparation preferably further comprises an auxiliary agent, wherein the auxiliary agent preferably comprises ascorbic acid, potassium dihydrogen phosphate and dipotassium hydrogen phosphate trihydrate; in the invention, the ascorbic acid is used as an auxiliary agent, so that the oxidation of EGCG can be effectively slowed down, the application effect of EGCG is increased, and the EGCG can be ensured to continuously improve the resistance of strawberry to gray mold; the potassium dihydrogen phosphate and the dipotassium hydrogen phosphate trihydrate are used as auxiliary agents, and the pH value of the preparation is adjusted after the potassium dihydrogen phosphate and the dipotassium hydrogen phosphate trihydrate are dissolved in water.
In the present invention, the functional preparation preferably comprises the following components in 100 mL:
more preferably, the following components are included:
the invention provides a preparation method of the functional preparation, which comprises the following steps: 1) dissolving potassium dihydrogen phosphate and dimethyl hydrogen phosphate trihydrate with water to obtain a salt solution; 2) mixing the epigallocatechin gallate and the ascorbic acid with the salt solution, and diluting to a constant volume to obtain the functional preparation.
In the specific implementation process of the invention, the potassium dihydrogen phosphate and the dimethyl hydrogen phosphate trihydrate are preferably mixed with part of water for dissolving, and then the epigallocatechin gallate and the ascorbic acid are mixed with a salt solution for constant volume. In the invention, the constant volume is preferably carried out by using water; the total amount of the water for dissolving the potassium dihydrogen phosphate and the dimethyl hydrogen phosphate trihydrate and the water for constant volume is the amount of the water in the functional preparation.
The invention provides application of the functional preparation in improving the gray mold resistance of strawberries. In the present invention, it is preferable that the functional preparation is diluted with water and then sprayed on the strawberry. In the present invention, the dilution factor is preferably 100 to 200 times, more preferably 140 to 160 times, and most preferably 150 times. In the invention, the spraying is preferably carried out in the morning of fine weather, the spraying is preferably carried out once every other day, the spraying is preferably carried out 3-8 times in total, and the amount of each spraying is preferably that the spraying is carried out until the foliage is wet and does not drip.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
The components and contents of the functional preparation in this example are as follows, per 100mL of the preparation:
the preparation is prepared according to the proportion, firstly, the potassium dihydrogen phosphate and the dipotassium hydrogen phosphate trihydrate are dissolved in water and then are mixed evenly, then, the epigallocatechin gallate and the ascorbic acid are poured into the mixed solution, and finally, the rest water is added to 100mL and mixed evenly.
Taking 100mL of the preparation, adding 15L of clear water, uniformly mixing, and spraying the mixture on the surfaces of strawberry leaves or fruits. The spraying is started at 15 days in 1 month in the year, and is carried out once every 1 day for 5 times in total. The test results are shown in table 1, the preparation can effectively relieve the gray mold of the strawberries after being applied, and compared with a control not applied with the preparation, the gray mold incidence of the strawberries applied with the preparation is reduced by 57.6%.
TABLE 1 disease index and control efficacy of strawberry Botrytis cinerea using clear water control and the functional agent in this example
Wherein: disease index ∑ (number of diseased plants at each stage × disease-grade value)/(total number of investigated plants × highest-grade value) × 100
Control effect (%) - (control disease index-treatment disease index)/control disease index × 100.
Example 2
The components and contents of the formulation in this example are as follows, per 100mL of formulation:
the preparation is prepared according to the proportion, firstly, the potassium dihydrogen phosphate and the dipotassium hydrogen phosphate trihydrate are dissolved in water and then are mixed evenly, then, the epigallocatechin gallate and the ascorbic acid are poured into the mixed solution, and finally, the rest water is added to 100mL and mixed evenly.
Taking 100mL of the preparation, adding 15L of clear water, uniformly mixing, and spraying the mixture on the surfaces of strawberry leaves or fruits. Spraying is started at 2 months and 15 days in the year, and is carried out once every 1 day for 6 times. The test results are shown in table 2, the preparation can effectively relieve the gray mold of the strawberries after being applied, and compared with a control not applied with the preparation, the gray mold incidence of the strawberries applied with the preparation is reduced by 66.5%.
TABLE 2 disease index and control efficacy of strawberry Botrytis cinerea using clear water control and the functional agent in this example
Example 3
The components and contents of the formulation in this example are as follows, per 100mL of formulation:
the preparation is prepared according to the proportion, firstly, the potassium dihydrogen phosphate and the dipotassium hydrogen phosphate trihydrate are dissolved in water and then are mixed evenly, then, the epigallocatechin gallate and the ascorbic acid are poured into the mixed solution, and finally, the rest water is added to 100mL and mixed evenly.
Taking 100mL of the preparation, adding 15L of clear water, uniformly mixing, and spraying the mixture on the surfaces of strawberry leaves or fruits. The spraying is started at 2 months and 15 days in the year, and is carried out once every 1 day for 6 times in total. The test results (table 3) show that the preparation can effectively relieve the gray mold of the strawberries after being applied, and compared with the control without the preparation, the incidence rate of the gray mold of the strawberries applied with the preparation is reduced by 32.9 percent.
TABLE 3 disease index and control efficacy of strawberry Botrytis cinerea using clear water control and the functional agent in this example
Comparative example
5 treatments were set, treatment 1 being a clean water control, treatment 2 being an equivalent amount of water to replace ascorbic acid in the functional preparation of example 1, treatment 3 being an equivalent amount of water to replace epigallocatechin gallate in the functional preparation of example 1, treatment 4 being a functional preparation of example 1, and treatment 5 being an equivalent amount of water to replace ascorbic acid and epigallocatechin gallate in the functional preparation of example 1.
Strawberries were sprayed in the same manner as in example 1, and the results are shown in table 4.
TABLE 4 disease index and controlling effect for treating 1-5 strawberry gray mold
Therefore, epigallocatechin gallate in the functional preparation is taken as a main active ingredient and plays a main role in inhibiting gray mold of strawberry, and the ascorbic acid, the monopotassium phosphate and the dipotassium hydrogen phosphate trihydrate can assist epigallocatechin gallate to play a better role and improve control effect.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. A functional preparation for improving strawberry gray mold resistance is characterized in that epigallocatechin gallate is used as an active ingredient; the functional preparation contains 0.5 to 1.2 percent of epigallocatechin gallate in percentage by mass;
the functional preparation is counted by 100mL and consists of the following components:
2. the functional preparation according to claim 1, wherein the content of epigallocatechin gallate in the functional preparation is 0.8-1.0% by mass.
3. The functional preparation according to claim 1, characterized in that it consists of, on a 100mL basis:
4. a method for preparing the functional preparation according to claim 3, comprising the steps of:
1) dissolving potassium dihydrogen phosphate and dipotassium hydrogen phosphate trihydrate with water to obtain a salt solution;
2) mixing the epigallocatechin gallate and the ascorbic acid with the salt solution, and diluting to a constant volume to obtain the functional preparation.
5. Use of the functional preparation according to any one of claims 1 to 3 for improving the gray mold resistance of strawberry.
6. Use according to claim 5, wherein the functional preparation is diluted with water and sprayed on the strawberries.
7. The use according to claim 6, wherein the dilution is by a factor of 100 to 200.
8. The use according to claim 7, wherein the dilution is in the range of 140 to 160 times.
9. The application of the plant foliar fertilizer as claimed in claim 6, wherein the spraying is carried out once every other day for 3-8 times in total, and the amount of each spraying is that the plant foliar fertilizer is moist and does not drip.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911140567.8A CN110786329B (en) | 2019-11-20 | 2019-11-20 | Functional preparation for improving strawberry gray mold resistance and preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911140567.8A CN110786329B (en) | 2019-11-20 | 2019-11-20 | Functional preparation for improving strawberry gray mold resistance and preparation method and application thereof |
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| CN110786329A CN110786329A (en) | 2020-02-14 |
| CN110786329B true CN110786329B (en) | 2021-06-08 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104095236A (en) * | 2014-07-21 | 2014-10-15 | 江苏天晟药业有限公司 | Health-care oral liquid capable of whitening and delaying aging and preparation method thereof |
| CN104413145A (en) * | 2013-08-19 | 2015-03-18 | 上海市建青实验学校 | Method for inhibiting colony growth of waxberry penicillium and gray mold |
| CN104472551A (en) * | 2014-12-16 | 2015-04-01 | 长阳勤劳农夫农产品有限公司 | Germ-immune type inductive agent and application thereof |
| CN105705013A (en) * | 2013-11-08 | 2016-06-22 | 诺维信生物农业公司 | Compositions and methods for treating pests |
| WO2019157061A2 (en) * | 2018-02-06 | 2019-08-15 | Novozymes Bioag A/S | Methods of protecting a plant from fungal pests |
-
2019
- 2019-11-20 CN CN201911140567.8A patent/CN110786329B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104413145A (en) * | 2013-08-19 | 2015-03-18 | 上海市建青实验学校 | Method for inhibiting colony growth of waxberry penicillium and gray mold |
| CN105705013A (en) * | 2013-11-08 | 2016-06-22 | 诺维信生物农业公司 | Compositions and methods for treating pests |
| CN104095236A (en) * | 2014-07-21 | 2014-10-15 | 江苏天晟药业有限公司 | Health-care oral liquid capable of whitening and delaying aging and preparation method thereof |
| CN104472551A (en) * | 2014-12-16 | 2015-04-01 | 长阳勤劳农夫农产品有限公司 | Germ-immune type inductive agent and application thereof |
| WO2019157061A2 (en) * | 2018-02-06 | 2019-08-15 | Novozymes Bioag A/S | Methods of protecting a plant from fungal pests |
Non-Patent Citations (1)
| Title |
|---|
| 不同浓度EGCG对NaCl胁迫下黄瓜种子萌发及其抗性的影响;李洋等;《浙江农业学报》;20180802(第07期) * |
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