CN110785654A - 系统级校准 - Google Patents

系统级校准 Download PDF

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Publication number
CN110785654A
CN110785654A CN201880040173.2A CN201880040173A CN110785654A CN 110785654 A CN110785654 A CN 110785654A CN 201880040173 A CN201880040173 A CN 201880040173A CN 110785654 A CN110785654 A CN 110785654A
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CN
China
Prior art keywords
image
channel
calibration
intensity
channels
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201880040173.2A
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English (en)
Chinese (zh)
Inventor
K·E·加尔沙
D·K·哈什曼
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Ventana Medical Systems Inc
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Ventana Medical Systems Inc
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Publication of CN110785654A publication Critical patent/CN110785654A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/90—Dynamic range modification of images or parts thereof
    • G06T5/92—Dynamic range modification of images or parts thereof based on global image properties
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/70—Circuitry for compensating brightness variation in the scene
    • H04N23/73—Circuitry for compensating brightness variation in the scene by influencing the exposure time
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10141—Special mode during image acquisition
    • G06T2207/10144—Varying exposure

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • Optics & Photonics (AREA)
  • Mathematical Physics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Signal Processing (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Microscoopes, Condenser (AREA)
CN201880040173.2A 2017-06-28 2018-06-26 系统级校准 Pending CN110785654A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762525818P 2017-06-28 2017-06-28
US62/525,818 2017-06-28
PCT/EP2018/067095 WO2019002281A1 (en) 2017-06-28 2018-06-26 CALIBRATION OF SYSTEM LEVEL

Publications (1)

Publication Number Publication Date
CN110785654A true CN110785654A (zh) 2020-02-11

Family

ID=62815014

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880040173.2A Pending CN110785654A (zh) 2017-06-28 2018-06-26 系统级校准

Country Status (5)

Country Link
US (2) US11747280B2 (https=)
EP (2) EP4488657A1 (https=)
JP (3) JP7068354B2 (https=)
CN (1) CN110785654A (https=)
WO (1) WO2019002281A1 (https=)

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US10890838B2 (en) * 2018-12-04 2021-01-12 University Of Massachusetts System and methods of fluorescence microscope calibration
US10895537B2 (en) * 2019-04-11 2021-01-19 Perkinelmer Health Sciences, Inc. Laser intensity calibration
EP3961194B1 (en) * 2020-08-25 2023-11-08 Korea Advanced Institute of Science and Technology Method and apparatus for multiplexed imaging of biomolecules through iterative unmixing of fluorophore signals
US20220252514A1 (en) * 2021-02-10 2022-08-11 Star Voltaic, LLC Fluorescent solid-state materials for optical calibration and methods thereof
EP4290220B1 (en) * 2022-06-10 2025-08-27 Leica Microsystems CMS GmbH Controller for an imaging device and method
TWI870685B (zh) * 2022-07-27 2025-01-21 泓偊科技股份有限公司 對光源自動校正之自動光學檢查設備
JP2025533369A (ja) * 2022-09-29 2025-10-07 イルミナ インコーポレイテッド 動的光学システム較正
US12062204B2 (en) * 2022-12-16 2024-08-13 Zomedica Inc. Methods and systems for slide imaging calibration
US20250132018A1 (en) * 2023-10-23 2025-04-24 Pramana, Inc. System and method of digitalizing a slide
WO2025115679A1 (ja) * 2023-12-01 2025-06-05 株式会社エビデント 光学顕微鏡及び性能判定方法
CN117764940B (zh) * 2023-12-19 2024-11-05 珠海圣美生物诊断技术有限公司 一种显微镜状态检测方法、装置、计算机设备及存储介质

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Publication number Priority date Publication date Assignee Title
CN119151830A (zh) * 2024-08-15 2024-12-17 华南师范大学 一种用于定量荧光共振能量转移显微成像的照明光场校正方法及系统

Also Published As

Publication number Publication date
US12320752B2 (en) 2025-06-03
JP2022119771A (ja) 2022-08-17
JP2024024626A (ja) 2024-02-22
EP3646006A1 (en) 2020-05-06
WO2019002281A1 (en) 2019-01-03
JP2020525772A (ja) 2020-08-27
JP7368539B2 (ja) 2023-10-24
US20200080940A1 (en) 2020-03-12
EP3646006B1 (en) 2024-10-23
JP7068354B2 (ja) 2022-05-16
EP4488657A1 (en) 2025-01-08
US20230375477A1 (en) 2023-11-23
US11747280B2 (en) 2023-09-05

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