CN110772528B

CN110772528B - Application of iris polysaccharide as medicine in preparing medicine for preventing and treating leptospirosis

Info

Publication number: CN110772528B
Application number: CN201911265637.2A
Authority: CN
Inventors: 曹永国; 宋宁; 吴殿君; 张文龙; 谢旭峰; 吕天宝; 王佳琪; 丁越; 张石磊
Original assignee: Jilin University
Current assignee: Jilin University
Priority date: 2019-12-11
Filing date: 2019-12-11
Publication date: 2023-03-14
Anticipated expiration: 2039-12-11
Also published as: CN110772528A

Abstract

The invention provides an application of tectori polysaccharide in preparing a medicament for preventing and treating leptospirosis, which adopts a standard substance of astragalus polysaccharide to inject the astragalus polysaccharide into golden yellow mice sensitive to leptospirosis in advance at a dose of 50mg/kg, can effectively control the infection of leptospirosis after infecting leptospirosis of 2X108/ml, and has a survival rate of 100 percent. The invention expands the medicinal range of the astragalus polysaccharide and provides a basis for developing new application of the iris polysaccharide.

Description

Application of iris polysaccharide as medicine in preparing medicine for preventing and treating leptospirosis

Technical Field

Leptospirosis (Leptospirosis) is a worldwide common zoonotic disease, called Leptospirosis for short, that is associated with occupational or living contacts and with exposure to leptospira, and is highly prevalent in flood-prone areas, and is particularly widespread in developing countries and tropical regions. The disease is susceptible to human and livestock, and the clinical manifestations after infection are various, so that diagnosis is not easy. The pathogen of the leptospirosis can continuously exist in the host, further spread to pollute the environment, infect other people and animals, and cause serious harm. Currently, the methods for preventing and treating leptospira are the injection of vaccines and the use of antibiotics.

Iris, namely Indian iris, fanglauca herbs, rhizoma anemarrhenae and the like, are originally produced in China and Japan, are perennial herbaceous plants with bitter, pungent and mild properties, and are common Chinese herbal medicines in China. Has the main functions of promoting blood circulation to remove blood stasis, dispelling wind and promoting diuresis, detoxifying and removing food retention. Can be used for treating traumatic injury, rheumatalgia, sore throat, food stagnation, abdominal distention, malaria, carbuncle, furuncle, toxic swelling, and traumatic hemorrhage. The research on iris polysaccharide in China is less, and the iris lactuca polysaccharide is especially the ananas comosus in the aspect of preventing and treating leptospira.

Background

The invention discloses application of tectori polysaccharide in preparation of a medicament for preventing and treating leptospirosis, provides new medical application of tectori polysaccharide, and belongs to the technical field of medicines.

Disclosure of Invention

The invention aims to provide application of tectori polysaccharide as a medicament in preparing medicaments for preventing and treating leptospirosis, including the prevention of leptospirosis and the treatment after infection. The application of the tectoridin can reduce the abuse of antibiotics and can also effectively prevent and control leptospira outbreak.

The invention aims to provide application of tectori polysaccharide as a medicament in preparing medicaments for preventing and treating leptospirosis, including the prevention of leptospirosis and treatment after infection.

The iris polysaccharide substance is used as a medicament for preventing and treating leptospirosis.

The iris polysaccharide substance is used as a medicine for preventing and treating leptospira, and is suitable for any pharmaceutically acceptable carrier.

The pharmaceutical preparation is standard liquid preparation of tectorigenin, and the dosage of the pharmaceutical preparation is 5mg/ml by dissolving and diluting tectorigenin powder with normal saline, namely 10mg/kg.

The tectorigenin can effectively control the infection of leptospira, reduce the inflammatory reaction of each organ and improve the survival rate of golden hamster to 100 percent.

The invention has the positive effects that:

provides a new medical application of the tectorigenin for preparing a medicine for preventing and treating leptospira; carrying out secondary development on the pharmacological action of the compound; the medicinal range of the iris polysaccharide is expanded; the invention can effectively reduce the use of antibiotics when treating and preventing leptospirosis, can also effectively prevent and control leptospirosis during outbreak, and has great effect on the healthy development of animal husbandry.

Description of the drawings:

FIG. 1 is a graph of the resistance of golden hamster to leptospira infection after injection of tectorigenin;

FIG. 2 is a graph of the scavenging effect of tectorigenin on leptospira within the body;

FIG. 3 shows the protective effect of tectorigenin on the major organs of golden hamster;

FIG. 4 shows that tectorigenin causes the expression level of important inflammatory factors in the body.

The specific implementation mode is as follows:

the present invention is further illustrated by the following examples, which do not limit the present invention in any way, and any modifications or changes which can be easily made by a person skilled in the art without departing from the technical solution of the present invention will fall within the scope of the claims of the present invention.

Example 1

The polysaccharide extract of Iris tectorum can be used as medicine for preventing and treating leptospirosis.

Example 2

The tectorigenin used as a medicine for preventing and treating leptospira is suitable for any pharmaceutically acceptable carrier.

Example 3

The pharmaceutical preparation is an iris polysaccharide standard liquid preparation, and the iris polysaccharide powder is dissolved and diluted by using normal saline according to dosage.

Example 4

The pharmaceutical preparation is standard liquid preparation of tectorigenin, and normal saline is used for dissolving and diluting tectorigenin powder according to dosage, wherein the dosage is 5mg/ml, and the dosage of the tectorigenin is 10mg/kg.

Test example 1

Application of tectorum polysaccharide in preventing leptospira infection

Experimental drugs:

the standard substance of iris polysaccharide was diluted with physiological saline at 5mg/ml and injected subcutaneously at a dose of 50mg/kg based on the body weight of golden hamster.

The experimental method comprises the following steps:

the experiment was divided into two groups, i.e., an experimental group and a control group, each of eight four-week-old golden hamster. The experimental group was injected with irises at a dose according to body weight one week before infection, and the control group was injected with physiological saline. One week later, 2 × 108 leptospira were intraperitoneally injected. And observing the survival rate.

The experimental results are as follows:

referring to fig. 1, the survival rate of the experimental group injected with iris polysaccharide was 100%, while all of the golden yellow mice of the control group died. Indicating that the tectorigenin can effectively prevent the infection of leptospira.

Test example 2

Iris polysaccharide has effect in clearing leptospira in organism

The experimental method comprises the following steps:

the golden hamster of the control group and the experimental group in the experimental example 1 was sampled, and the liver, kidney and lung of each group of golden hamster were collected, and subjected to DNA extraction of leptospira in the tissue, and then to a fluorescent quantitative PCR experiment to analyze the load of leptospira in each organ of golden hamster.

The experimental results are as follows:

referring to fig. 2, compared to the control group injected with physiological saline only, the mice injected with tectori polysaccharide had a significant decrease in the leptospira loading in liver, kidney and lung.

Test example 3

The control and experimental groups of golden hamster in test example 1 were sampled for liver, kidney and lung, RNA was extracted, and then fluorescence quantitative PCR was performed to analyze the expression of Tlr2, tlr4, IL-1. Beta. And iNOS 2 and 5 days after the attack of bacteria in each organ of golden hamster.

The experimental results are as follows:

referring to fig. 3, in the experimental group injected with irises, the mRNA expression of TLR2 in the liver and kidney was significantly higher at 2dpi and lower at 5dpi, compared to the control group. However, in the experimental group, the expression of TLR2 mRNA in lung was significantly reduced. Meanwhile, in the experimental group, the mRNA expression of TLR4 is reduced or induced to be reduced at 2dpi, and is obviously reduced at 5 dpi. In the experiment group injected with tectoridin, the mRNA expression of IL-1. Beta. Was higher at 2dpi and lower at 5dpi in all organs, compared to the infection control group. These results indicate that irises caused mRNA expression of large amounts of TLR2 and IL-1 β in the organ at 2dpi, and reduced expression of TLR2, TLR4 and IL-1 β at 5 dpi. After the irises attack bacteria, 2dpi can lead inflammation storm to arrive early, and the inflammation storm is reduced at 5dpi, so that the irises have a protective effect on golden hamster.

Test example 4

The golden hamster of the control group and the golden hamster of the experimental group in test example 1 was sampled for liver, kidney and lung to prepare pathological sections, and HE staining was performed. Observation was performed by an optical microscope at 200 times.

The experimental results are as follows:

referring to fig. 4, the liver, kidney and lung of the golden hamster of the control group all have different degrees of bleeding and inflammatory lesions, while the pathological changes of the liver, kidney and lung of the golden hamster of the experimental group are not particularly obvious and have substantially no bleeding points and inflammatory lesions.

Claims

1. Use of tectori polysaccharide in preparing medicine for preventing and treating leptospira disease is provided.