CN110771429A - Method for rapidly cultivating poria cocos sporocarp by trichoderma induction - Google Patents

Abstract

The invention discloses a method for rapidly cultivating poria cocos sporocarp by trichoderma induction. The method takes bottle cultivation of the poria cocos sporocarp, takes the wood chip as a growth carrier of the poria cocos sporocarp, adopts trichoderma spores for stimulation, is beneficial to early formation of the sporocarp, only needs 22 days for fast growth of the sporocarp, and is earlier than the common cultivation by more than 15 days for fruiting; the culture medium has good air permeability, fast hypha growth, sufficient nutrition and high tuckahoe fruiting body formation rate (100 percent of fruiting can be realized), and the cultivation technology that tuckahoe fruiting bodies do not use pine is realized, thus being beneficial to the full cultivation, promoting the development of traditional Chinese medicine enterprises which utilize tailing tuckahoe raw materials of various woods, and bringing about good ecological effect, economic effect and social effect.

Description

Method for rapidly cultivating poria cocos sporocarp by trichoderma induction

Technical Field

The invention belongs to the technical field of biology, and particularly relates to a method for rapidly cultivating poria cocos sporocarp by trichoderma induction.

Background

The morphological characteristics of the fruiting body are important basis for classifying tuckahoe, the formation of tuckahoe fruiting body in nature is limited by seasons and environmental conditions, and stable fruiting body is difficult to obtain under laboratory conditions. Xiongjihas been cultured with mycelium obtained by separation of sclerotium tissue of Poria cocos to form a fruiting body, Nippon Fuyong Bao obtained pure culture with test tube and triangular flask and wood chip culture to obtain a fruiting body of Poria cocos, Fuyung Bao obtained optimum solid culture conditions for the fruiting body of Poria cocos by comparing different solid culture media, Houjun obtained the fruiting body of Poria cocos by using the culture medium of Poria cocos. The time for culturing the fruit body is longer, the shortest time is more than 37 days, the formation of the fruit body of 100 percent cannot be ensured, the production cost is increased, and the labor efficiency is reduced.

Disclosure of Invention

In order to solve the technical problems, the invention discloses a method for rapidly cultivating poria cocos sporocarp by trichoderma induction. The method takes bottle cultivation of the poria cocos sporocarp, takes the wood chip as a growth carrier of the poria cocos sporocarp, adopts trichoderma spores for stimulation, is beneficial to early formation of the sporocarp, only needs 22 days for fast growth of the sporocarp, and is earlier than the common cultivation by more than 15 days for fruiting; the culture medium has good air permeability, fast hypha growth, sufficient nutrition and high tuckahoe fruiting body formation rate (100 percent of fruiting can be realized), and the cultivation technology that tuckahoe fruiting bodies do not use pine is realized, thus being beneficial to the full cultivation, promoting the development of traditional Chinese medicine enterprises which utilize tailing tuckahoe raw materials of various woods, and bringing about good ecological effect, economic effect and social effect.

The purpose of the invention is realized by the following technical scheme:

a method for rapidly cultivating Poria cocos fruiting bodies by adopting trichoderma induction comprises the following steps:

step one, preparing a poria cocos sporocarp solid culture medium;

and step two, adding a poria cocos sporophore solid culture medium into a tissue culture bottle, then inoculating a fungus cake containing poria cocos hyphae into the poria cocos sporophore solid culture medium, carrying out dark culture at the temperature of 25-28 ℃, adding 10-1000 trichoderma spores per bottle into the tissue culture bottle with the poria cocos hyphae growing in half of the volume of the solid culture medium, continuously culturing for 10-15 days at the temperature of 28-30 ℃, then, causing hyphae kinks to appear, gradually differentiating protrusions, and continuously culturing for 8-13 days under the condition that the illumination intensity is 300 plus 700lux to obtain dentate poria cocos sporophore.

In a further improvement, the poria cocos fruiting body solid culture medium comprises, by weight, 70% -80% of sawdust, 15% -25% of bran, 1% -5% of corn and 1% -3% of sucrose.

The further improvement comprises the following steps:

adding pine sawdust, bran, corn, cane sugar and water into a tissue culture bottle, wherein the material-liquid ratio is 1:3, the loading amount is 1/3 with the volume, sterilizing at 121 ℃ for 2h, cooling, inoculating a fungus cake containing poria cocos hyphae with the diameter of 5-8mm, carrying out dark culture at 25-28 ℃, and culturing for 4-7 days to ensure that the poria cocos hyphae grow to be full of half of the volume of a solid culture medium for later use;

step two, adjusting the concentration of the trichoderma spore suspension to 1000-10000/mL for later use;

step three, adding 10-1000 trichoderma spores per bottle into a tissue culture bottle with the poria hyphae growing in half of the volume of the solid culture medium until the part is not covered by the poria hyphae temporarily, continuously culturing for 10-15 days at the culture temperature of 28-30 ℃, then kinking the hyphae, gradually differentiating the protrusions, and continuously culturing for 8-13 days under the illumination intensity of 300 plus 700lux to obtain dentate poria cocos sporocarp which is strong; the fruiting bodies can be induced in each bottle, and the fruiting rate reaches 100%.

Drawings

FIG. 1 is a flow chart of the present invention;

FIG. 2 is a diagram of Poria cocos fruiting body.

Detailed Description

The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.

As shown in FIG. 1, a method for rapidly cultivating Poria cocos fruiting body by Trichoderma induction comprises the following steps:

adding pine sawdust, bran, corn, cane sugar and water into a tissue culture bottle, wherein the material-liquid ratio is 1:3, the loading amount is 1/3 with the volume, sterilizing at 121 ℃ for 2h, cooling, inoculating a fungus cake containing poria cocos hyphae with the diameter of 5-8mm, carrying out dark culture at 25-28 ℃, and culturing for 4-7 days to ensure that the poria cocos hyphae grow to be full of half of the volume of a solid culture medium for later use;

step two, adjusting the concentration of the trichoderma spore suspension to 1000-10000/mL for later use;

step three, adding 10-1000 trichoderma spores per bottle into a tissue culture bottle with poria hyphae growing in half of the volume of the solid culture medium until the part is not covered by the poria hyphae temporarily, culturing for 10-15 days at the culture temperature of 28-30 ℃, then generating hypha kinks and gradually differentiating the protrusions, and continuously culturing for 8-13 days under the condition that the illumination intensity is 300 plus 700lux to obtain dentate poria cocos sporocarp which is strong; fruiting bodies can be induced from each bottle, and fruiting rate reaches 100%, as shown in FIG. 2.

While embodiments of the invention have been described above, it is not limited to the applications set forth in the specification and the embodiments, which are fully applicable to various fields of endeavor for which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the specific details shown and described herein without departing from the general concept defined by the claims and their equivalents.

Claims (3)

1. A method for rapidly cultivating poria cocos sporocarp by trichoderma induction is characterized by comprising the following steps:

step one, preparing a poria cocos sporocarp solid culture medium;

and step two, adding a poria cocos sporophore solid culture medium into a tissue culture bottle, then inoculating a fungus cake containing poria cocos hyphae into the poria cocos sporophore solid culture medium, carrying out dark culture at the temperature of 25-28 ℃, adding 10-1000 trichoderma spores per bottle into the tissue culture bottle with the poria cocos hyphae growing in half of the volume of the solid culture medium, continuously culturing for 10-15 days at the temperature of 28-30 ℃, then, causing hyphae kinks to appear, gradually differentiating protrusions, and continuously culturing for 8-13 days under the condition that the illumination intensity is 300 plus 700lux to obtain dentate poria cocos sporophore.

2. The method for rapidly cultivating a poria cocos fruiting body through trichoderma induction according to claim 1, wherein the poria cocos fruiting body solid culture medium comprises 70% -80% of wood flour, 15% -25% of bran, 1% -5% of corn and 1% -3% of sucrose in percentage by weight.

3. The method for inducing the rapid culture of poria cocos fruiting body using trichoderma as claimed in claim 1, comprising the steps of:

adding pine sawdust, bran, corn, cane sugar and water into a tissue culture bottle, wherein the material-liquid ratio is 1:3, the loading amount is 1/3 with the volume, sterilizing at 121 ℃ for 2h, cooling, inoculating a fungus cake containing poria cocos hyphae with the diameter of 5-8mm, carrying out dark culture at 25-28 ℃, and culturing for 4-7 days to ensure that the poria cocos hyphae grow to be full of half of the volume of a solid culture medium for later use;

step two, adjusting the concentration of the trichoderma spore suspension to 1000-10000/mL for later use;

step three, adding 10-1000 trichoderma spores per bottle into a tissue culture bottle with the poria hyphae growing in half of the volume of the solid culture medium until the part is not covered by the poria hyphae temporarily, continuously culturing for 10-15 days at the culture temperature of 28-30 ℃, then kinking the hyphae, gradually differentiating the protrusions, and continuously culturing for 8-13 days under the illumination intensity of 300 plus 700lux to obtain dentate poria cocos sporocarp which is strong; the fruiting bodies can be induced in each bottle, and the fruiting rate reaches 100%.