CN110755532A - 一种对大蒜皮进行提取的方法、提取得到的大蒜皮提取物及其用途 - Google Patents
一种对大蒜皮进行提取的方法、提取得到的大蒜皮提取物及其用途 Download PDFInfo
- Publication number
- CN110755532A CN110755532A CN201911103402.3A CN201911103402A CN110755532A CN 110755532 A CN110755532 A CN 110755532A CN 201911103402 A CN201911103402 A CN 201911103402A CN 110755532 A CN110755532 A CN 110755532A
- Authority
- CN
- China
- Prior art keywords
- garlic skin
- garlic
- ethanol
- extracting
- extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 240000002234 Allium sativum Species 0.000 title claims abstract description 101
- 235000004611 garlic Nutrition 0.000 title claims abstract description 100
- 239000000284 extract Substances 0.000 title claims abstract description 28
- 238000000605 extraction Methods 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 108
- 239000000843 powder Substances 0.000 claims abstract description 44
- 239000000243 solution Substances 0.000 claims abstract description 43
- 230000002378 acidificating effect Effects 0.000 claims abstract description 30
- 238000006243 chemical reaction Methods 0.000 claims abstract description 15
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 14
- 238000002156 mixing Methods 0.000 claims abstract description 11
- 239000000469 ethanolic extract Substances 0.000 claims abstract description 7
- 239000011259 mixed solution Substances 0.000 claims abstract description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 27
- 235000013305 food Nutrition 0.000 claims description 6
- 238000002386 leaching Methods 0.000 claims description 6
- 239000002537 cosmetic Substances 0.000 claims description 5
- 239000001509 sodium citrate Substances 0.000 claims description 5
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000002137 ultrasound extraction Methods 0.000 claims description 3
- 239000000825 pharmaceutical preparation Substances 0.000 claims 1
- 229940127557 pharmaceutical product Drugs 0.000 claims 1
- 239000003963 antioxidant agent Substances 0.000 abstract description 5
- 229930014626 natural product Natural products 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 description 15
- 238000011160 research Methods 0.000 description 6
- 235000010208 anthocyanin Nutrition 0.000 description 5
- 229930002877 anthocyanin Natural products 0.000 description 5
- 239000004410 anthocyanin Substances 0.000 description 5
- 150000004636 anthocyanins Chemical class 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 238000001291 vacuum drying Methods 0.000 description 5
- 238000005303 weighing Methods 0.000 description 5
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 4
- 235000006708 antioxidants Nutrition 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 229930003935 flavonoid Natural products 0.000 description 4
- 235000017173 flavonoids Nutrition 0.000 description 4
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 229930003944 flavone Natural products 0.000 description 3
- 150000002212 flavone derivatives Chemical class 0.000 description 3
- 235000011949 flavones Nutrition 0.000 description 3
- 150000002215 flavonoids Chemical class 0.000 description 3
- 239000000049 pigment Substances 0.000 description 3
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 3
- 239000013543 active substance Substances 0.000 description 2
- 238000002481 ethanol extraction Methods 0.000 description 2
- -1 flavonoid compounds Chemical class 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 2
- 230000002000 scavenging effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000234282 Allium Species 0.000 description 1
- 241000383638 Allium nigrum Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 229940079877 pyrogallol Drugs 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8962—Allium, e.g. garden onion, leek, garlic or chives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/18—Antioxidants, e.g. antiradicals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Birds (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Cosmetics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
本发明涉及天然化合物提取技术领域,具体涉及一种对大蒜皮进行提取的方法、提取得到的大蒜皮提取物及其用途。该对大蒜皮进行提取的方法,其特征在于,包括如下步骤:(1)向盛有大蒜皮粉末的反应瓶中加入酸性乙醇对所述大蒜皮粉末进行提取,提取结束后分离得到酸性乙醇提取液;(2)向所述反应瓶中加入碱性乙醇,使其与大蒜皮粉末混合后混合液的pH=6.5‑8.0,并对所述大蒜皮粉末进行第二次提取,得到第二提取液;(3)向反应瓶中加入乙醇,对大蒜皮粉末进行第三次提取,得到第三提取液;(4)浓缩所述酸性乙醇提取液、第二提取液和/或第三提取液得到大蒜皮提取物。采用该方法提取得到的大蒜皮提取物的抗氧剂性能更好。
Description
技术领域
本发明涉及天然化合物提取技术领域,具体涉及一种对大蒜皮进行提取的方法、提取得到的大蒜皮提取物及其用途。
背景技术
大蒜为百合科葱属植物蒜(Allium Sativum L.)的鳞茎,在我国多个地区广泛种植,是一种药食两用的植物资源。大蒜各部位含有多种活性成分,被誉为“地里长出来的抗生素”。诸多研究表明,大蒜具有抗菌消炎、防治癌症、抗氧化、增强免疫力等生理活性,已广泛应用于医药、食品、化妆品等领域。相比之下,大蒜皮作为加工副产物常被抛弃,造成了资源浪费。
研究表明,大蒜皮中含有丰富的生物活性物质,紫蒜皮和黑蒜皮中还含有花色苷等黄酮类化合物,具有良好的抗氧化活性,可用于食品、药品、化妆品等领域。因此,对大蒜皮提取物进行研究不仅可减少资源浪费和环境污染,而且有助于提高企业的经济效益。
文献《大蒜皮总黄酮的提取及其抗氧化作用的研究》对从大蒜皮中提取黄酮的工艺进行了研究,结果表明,超声波乙醇浸提法提取大蒜皮总黄酮的最佳提取工艺为乙醇浓度80%,超声时间60min,超声温度80℃,料液比1:40(m/V);抗氧化能力评估实验表明:大蒜皮总黄酮具有良好的清除超氧离子和羟基自由基的能力。虽然该研究表明,在超声时间为50min内时,大蒜皮总黄酮的得率不断增大,但提取过程中大蒜皮中的花色苷会被破坏,造成其抗氧化活性的损失。
发明内容
本发明提供一种对大蒜皮进行提取的方法,以解决现有技术中采用超声提取法对大蒜皮进行提取时造成的花色苷类物质被破坏而造成的抗氧化活性降低的问题。
本发明的对大蒜皮进行提取的方法采用如下技术方案:一种对大蒜皮进行提取的方法,包括如下步骤:(1)向盛有大蒜皮粉末的反应瓶中加入酸性乙醇对所述大蒜皮粉末进行提取,提取结束后分离得到酸性乙醇提取液;(2)向所述反应瓶中加入碱性乙醇,使其与大蒜皮粉末混合后混合液的pH=6.5-8.0,并对所述大蒜皮粉末进行第二次提取,得到第二提取液;(3)向反应瓶中加入乙醇,对大蒜皮粉末进行第三次提取,得到第三提取液;(4)浓缩所述酸性乙醇提取液、第二提取液和/或第三提取液得到大蒜皮提取物。
优选的,所述酸性乙醇由体积分数为60%-80%的乙醇和柠檬酸配制而成。
优选的,所述碱性乙醇由体积分数为60%-80%的乙醇和柠檬酸钠配制而成。
优选的,采用浸提法对所述大蒜皮粉末进行提取,提取温度为50-80℃,提取时间2-4h。
优选的,所述步骤(2)和步骤(3)中采用超声提取法进行提取,超声功率为150-350W,温度为20-40℃,提取时间10-20min。
本发明还提供了采用如上述任意一项所述的方法制备得到的大蒜皮提取物。
本发明还提供了如上所述的大蒜皮提取物的用途,具体技术方案为:所述大蒜皮提取物在制备具有抗氧化活性的化妆品、食品或药品中的应用。
本发明的有益效果是:本发明在对大蒜皮进行提取时,通过采用酸性乙醇对大蒜皮进行提取,可有效保护在提取过程中大蒜皮所具有的色素(花色苷)不被破坏,维持其抗氧化活性;之后再分别加入碱性乙醇,调节pH,以使具有抗氧化活性的黄酮类物质更多的进入乙醇溶液中,提高抗氧化活性物质的提取率。
采用柠檬酸配制酸性乙醇,有助于大蒜皮色素的稳定。
本发明的对大蒜皮进行提取的提取方法简单,得到的大蒜皮提取物的抗氧化活性高,可用于制备具有抗氧化活性的化妆品、食品、药品等。
具体实施方式
下面将结合本发明的具体实施例对本发明的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。
一、制备大蒜皮提取物
实施例1
将大蒜皮清洗、干燥后粉碎成大蒜皮粉末备用。
(1)称取5g大蒜皮粉末置于反应瓶中,向反应瓶中加入200mL酸性乙醇(体积分数为70%的乙醇,用柠檬酸调节pH至4.0),70℃浸提3h,过滤,得到酸性乙醇提取液;
(2)向经酸性乙醇提取后的大蒜皮粉末中加入200mL含有柠檬酸钠的碱性乙醇(体积分数为70%),使其与大蒜皮粉末混合后测pH=7.0;设定超声功率200W、温度30℃提取10min,过滤,得到第二提取液;
(3)向经碱性乙醇提取后的大蒜皮粉末中加入200mL体积分数为70%的乙醇,设定超声功率200W、温度30℃提取15min,过滤,得到第三提取液;
(4)合并酸性提取液、第二提取液和第三提取液,减压浓缩、真空干燥得到0.095g大蒜皮提取物。
实施例2
将大蒜皮清洗、干燥后粉碎成大蒜皮粉末备用。
(1)称取5g大蒜皮粉末置于反应瓶中,向反应瓶中加入200mL酸性乙醇(体积分数为60%的乙醇,用柠檬酸调节pH至4.0),50℃浸提4h,过滤,得到酸性乙醇提取液;
(2)向经酸性乙醇提取后的大蒜皮粉末中加入200mL含有柠檬酸钠的碱性乙醇(体积分数为80%),使其与大蒜皮粉末混合后测pH=6.5;设定超声功率350W、温度20℃提取15min,过滤,得到第二提取液;
(3)向经碱性乙醇提取后的大蒜皮粉末中加入200mL体积分数为60%的乙醇,设定超声功率200W、温度20℃提取10min,过滤,得到第三提取液;
(4)合并酸性提取液、第二提取液和第三提取液,减压浓缩、真空干燥得到0.093g大蒜皮提取物。
实施例3
将大蒜皮清洗、干燥后粉碎成大蒜皮粉末备用。
(1)称取5g大蒜皮粉末置于反应瓶中,向反应瓶中加入200mL酸性乙醇(体积分数为80%的乙醇,用柠檬酸调节pH至4.0),80℃浸提2h,过滤,得到酸性乙醇提取液;
(2)向经酸性乙醇提取后的大蒜皮粉末中加入200mL含有柠檬酸钠的碱性乙醇(体积分数为80%),使其与大蒜皮粉末混合后测pH=8.0;设定超声功率150W、温度40℃提取10min,过滤,得到第二提取液;
(3)向经碱性乙醇提取后的大蒜皮粉末中加入200mL体积分数为70%的乙醇,设定超声功率200W、温度30℃提取20min,过滤,得到第三提取液;
(4)合并酸性提取液、第二提取液和第三提取液,减压浓缩、真空干燥得到0.091g大蒜皮提取物。
对比例1
将大蒜皮清洗、干燥后粉碎成大蒜皮粉末备用。
(1)称取5g大蒜皮粉末置于反应瓶中,向反应瓶中加入200mL酸性乙醇(体积分数为70%的乙醇,用柠檬酸调节pH至4.0),70℃浸提3h,过滤,得到酸性乙醇提取液;
(2)向经酸性乙醇提取后的大蒜皮粉末中加入200mL酸性乙醇(体积分数为70%的乙醇,用柠檬酸调节pH至4.0),设定超声功率200W、温度30℃提取10min,过滤,得到第二提取液;
(3)向经步骤(2)提取后的大蒜皮粉末中加入200mL酸性乙醇(体积分数为70%的乙醇,用柠檬酸调节pH至4.0),设定超声功率200W、温度30℃提取15min,过滤,得到第三提取液;
(4)合并酸性提取液、第二提取液和第三提取液,减压浓缩、真空干燥得到0.083g大蒜皮提取物。
对比例2
将大蒜皮清洗、干燥后粉碎成大蒜皮粉末备用。
(1)称取5g大蒜皮粉末置于反应瓶中,向反应瓶中加入200mL乙醇(体积分数为70%),70℃浸提3h,过滤,得到第一提取液;
(2)向经步骤(1)提取后的大蒜皮粉末中加入200mL乙醇(体积分数为70%的乙醇),设定超声功率200W、温度30℃提取10min,过滤,得到第二提取液;
(3)向经步骤(2)提取后的大蒜皮粉末中加入200mL乙醇(体积分数为70%的乙醇),设定超声功率200W、温度30℃提取15min,过滤,得到第三提取液;
(4)合并第一提取液、第二提取液和第三提取液,减压浓缩、真空干燥得到0.071g大蒜皮提取物。
二、对提取得到的大蒜皮提取物的抗氧化性能进行检测
1.测定方法:利用邻苯三酚氧化法测定大蒜皮提取物清除超氧阴离子的能力,在320nm波长下测定0.3mg/mL大蒜皮提取物的吸光值,计算超氧阴离子的清除率。计算公式如下:
其中:A0为添加蒸馏水的吸光度;An为添加抗氧化活性物质后的吸光度。
测定结果见下表1:
表1制备得到的大蒜皮提取物的超氧阴离子清除率
样品(0.3mg/mL) | 实施例1 | 实施例2 | 实施例3 | 对比例1 | 对比例2 |
超氧阴离子清除率 | 88.1% | 88.5% | 88.2% | 85.2% | 83.5% |
以上所述仅为本发明的较佳实施例而已,并不用以限制本发明,凡在本发明的精神和原则之内,所作的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。
Claims (7)
1.一种对大蒜皮进行提取的方法,其特征在于,包括如下步骤:(1)向盛有大蒜皮粉末的反应瓶中加入酸性乙醇对所述大蒜皮粉末进行提取,提取结束后分离得到酸性乙醇提取液;(2)向所述反应瓶中加入碱性乙醇,使其与大蒜皮粉末混合后混合液的pH=6.5-8.0,并对所述大蒜皮粉末进行第二次提取,得到第二提取液;(3)向反应瓶中加入乙醇,对大蒜皮粉末进行第三次提取,得到第三提取液;(4)浓缩所述酸性乙醇提取液、第二提取液和/或第三提取液得到大蒜皮提取物。
2.根据权利要求1所述的对大蒜皮进行提取的方法,其特征在于,所述酸性乙醇由体积分数为60%-80%的乙醇和柠檬酸配制而成。
3.根据权利要求1所述的对大蒜皮进行提取的方法,其特征在于,所述碱性乙醇由体积分数为60%-80%的乙醇和柠檬酸钠配制而成。
4.根据权利要求1或2所述的对大蒜皮进行提取的方法,其特征在于,采用浸提法对所述大蒜皮粉末进行提取,提取温度为50-80℃,提取时间2-4h。
5.根据权利要求1或3所述的对大蒜皮进行提取的方法,其特征在于,所述步骤(2)和步骤(3)中采用超声提取法进行提取,超声功率为150-350W,温度为20-40℃,提取时间10-20min。
6.采用如权利要求1-5中任意一项所述的方法制备得到的大蒜皮提取物。
7.根据权利要求6所述的大蒜皮提取物的用途,其特征在于,所述大蒜皮提取物在制备具有抗氧化活性的化妆品、食品或药品中的应用。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201911103402.3A CN110755532A (zh) | 2019-11-12 | 2019-11-12 | 一种对大蒜皮进行提取的方法、提取得到的大蒜皮提取物及其用途 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201911103402.3A CN110755532A (zh) | 2019-11-12 | 2019-11-12 | 一种对大蒜皮进行提取的方法、提取得到的大蒜皮提取物及其用途 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN110755532A true CN110755532A (zh) | 2020-02-07 |
Family
ID=69337504
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201911103402.3A Pending CN110755532A (zh) | 2019-11-12 | 2019-11-12 | 一种对大蒜皮进行提取的方法、提取得到的大蒜皮提取物及其用途 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110755532A (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112957417A (zh) * | 2021-03-02 | 2021-06-15 | 上海芝道实业有限公司 | 一种具有养生效果的紫色大蒜皮粉末的制备方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106418111A (zh) * | 2016-10-08 | 2017-02-22 | 江苏大学 | 一种来源于大蒜秸秆的抗氧化提取物及其制备方法 |
-
2019
- 2019-11-12 CN CN201911103402.3A patent/CN110755532A/zh active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106418111A (zh) * | 2016-10-08 | 2017-02-22 | 江苏大学 | 一种来源于大蒜秸秆的抗氧化提取物及其制备方法 |
Non-Patent Citations (3)
Title |
---|
吴冬青 等: "大蒜皮红色素理化性质研究", 《食品工业科技》 * |
姜薇薇: "大蒜皮总黄酮的提取及其抗氧化作用的研究", 《中国调味品》 * |
李亚利 等: "大蒜皮提取物抗氧化活性初步研究", 《食品科技》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112957417A (zh) * | 2021-03-02 | 2021-06-15 | 上海芝道实业有限公司 | 一种具有养生效果的紫色大蒜皮粉末的制备方法 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106955250B (zh) | 一种利用葡萄酒糟制备面膜的方法及根据此方法得到的一种面膜 | |
CN101897656A (zh) | 雪莲培养物作为新原料在日化用品中的应用 | |
CN103251532A (zh) | 一种含茶树花的茶花护肤霜 | |
CN105147550A (zh) | 一种含有葛仙米提取物的美白保湿面膜液及其制备方法 | |
AU2020101467A4 (en) | Preparation method of lycium ruthenicum murr spray powder capsule | |
CN107737061B (zh) | 一种化妆品组合物及其制备方法 | |
CN105001348A (zh) | 一种高得率高岩藻糖比例的岩藻多糖的提取方法 | |
CN110755532A (zh) | 一种对大蒜皮进行提取的方法、提取得到的大蒜皮提取物及其用途 | |
CN106344429A (zh) | 一种金针菇精华液及其制备方法 | |
CN109810812B (zh) | 一种藏红花氨基酸皂及其制备方法 | |
CN104523500A (zh) | 一种含有双效植物活性成分的组合物及其制备工艺 | |
CN107468568A (zh) | 一种具有美白防晒活性的柿叶多酚及其制备方法和应用 | |
CN109744540A (zh) | 一种黑蒜中类黑精的提取方法 | |
CN112107522A (zh) | 美白组合物、美白面膜及其制备方法 | |
CN104921997B (zh) | 一种牡丹花超高压提取法及其制备的美白、抗氧化精华液 | |
CN108721192A (zh) | 一种天然植物提取精华面膜液及其提取工艺 | |
CN108653037B (zh) | 皮肤调理剂组合物、其应用及美白面膜 | |
CN107913203B (zh) | 一种银耳保湿抗皱剂及其制备方法和应用 | |
KR0168485B1 (ko) | 산성다당체를 다량 함유하는 홍삼엑기스 제조방법 | |
CN103751061A (zh) | 番茄叶提取物及在化妆品中的应用 | |
CN108542823A (zh) | 一种美白淡斑精华素微胶囊及其制备方法 | |
CN105902577A (zh) | 低糖蛹虫草口服片剂及其制备方法 | |
CN105596230A (zh) | 一种鱼胶原蛋白保湿面膜及其制备方法 | |
CN105496885A (zh) | 一种含植物元素的化妆品及其生产方法 | |
CN112545943B (zh) | 一种包含植物提取物的sod类化妆品添加剂 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200207 |
|
RJ01 | Rejection of invention patent application after publication |