CN110754468A - Screening method and application of striped rice borer control drug penetrant - Google Patents
Screening method and application of striped rice borer control drug penetrant Download PDFInfo
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- 241000426497 Chilo suppressalis Species 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 28
- 239000003814 drug Substances 0.000 title claims abstract description 24
- 238000012216 screening Methods 0.000 title claims abstract description 12
- 229940079593 drug Drugs 0.000 title claims abstract description 10
- 241000209094 Oryza Species 0.000 claims abstract description 53
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 53
- 235000009566 rice Nutrition 0.000 claims abstract description 53
- 239000007788 liquid Substances 0.000 claims abstract description 39
- 230000000149 penetrating effect Effects 0.000 claims abstract description 14
- 230000003405 preventing effect Effects 0.000 claims abstract description 12
- 239000003086 colorant Substances 0.000 claims abstract description 10
- 239000000126 substance Substances 0.000 claims abstract description 10
- 241000758794 Asarum Species 0.000 claims abstract description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 22
- 238000004040 coloring Methods 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000000080 wetting agent Substances 0.000 claims description 6
- 239000011358 absorbing material Substances 0.000 claims description 4
- 238000009966 trimming Methods 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 230000002745 absorbent Effects 0.000 claims 1
- 239000002250 absorbent Substances 0.000 claims 1
- 239000002357 osmotic agent Substances 0.000 claims 1
- 230000002195 synergetic effect Effects 0.000 abstract description 11
- 230000001276 controlling effect Effects 0.000 abstract description 8
- 239000012752 auxiliary agent Substances 0.000 abstract description 6
- 230000002265 prevention Effects 0.000 abstract description 3
- 230000035515 penetration Effects 0.000 description 20
- 230000000694 effects Effects 0.000 description 9
- 239000000575 pesticide Substances 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- 239000013043 chemical agent Substances 0.000 description 6
- 238000001764 infiltration Methods 0.000 description 5
- 230000008595 infiltration Effects 0.000 description 5
- 239000000976 ink Substances 0.000 description 5
- AMFGTOFWMRQMEM-UHFFFAOYSA-N triazophos Chemical compound N1=C(OP(=S)(OCC)OCC)N=CN1C1=CC=CC=C1 AMFGTOFWMRQMEM-UHFFFAOYSA-N 0.000 description 5
- 230000035699 permeability Effects 0.000 description 4
- 239000005660 Abamectin Substances 0.000 description 3
- 229950008167 abamectin Drugs 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 150000001412 amines Chemical class 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- 231100000225 lethality Toxicity 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- IBSREHMXUMOFBB-JFUDTMANSA-N 5u8924t11h Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1[C@@H](OC)C[C@H](O[C@@H]2C(=C/C[C@@H]3C[C@@H](C[C@@]4(O3)C=C[C@H](C)[C@@H](C(C)C)O4)OC(=O)[C@@H]3C=C(C)[C@@H](O)[C@H]4OC\C([C@@]34O)=C/C=C/[C@@H]2C)/C)O[C@H]1C.C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 IBSREHMXUMOFBB-JFUDTMANSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- MBNMHBAJUNHZRE-UHFFFAOYSA-M thiosultap monosodium Chemical compound [Na+].OS(=O)(=O)SCC(N(C)C)CSS([O-])(=O)=O MBNMHBAJUNHZRE-UHFFFAOYSA-M 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- -1 abamectin compound Chemical class 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- QXAITBQSYVNQDR-ZIOPAAQOSA-N amitraz Chemical compound C=1C=C(C)C=C(C)C=1/N=C/N(C)\C=N\C1=CC=C(C)C=C1C QXAITBQSYVNQDR-ZIOPAAQOSA-N 0.000 description 1
- 229960002587 amitraz Drugs 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/26—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-nitrogen bonds
- A01N57/30—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-nitrogen bonds containing aromatic radicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/15—Medicinal preparations ; Physical properties thereof, e.g. dissolubility
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Abstract
The invention discloses a screening method of a striped rice borer prevention and control drug penetrant, which is characterized in that a colorant is added into different penetrant liquids with the same concentration, and the time of each penetrant penetrating through a rice stem with a specific length is measured, wherein the penetrant liquid with short penetrating time has a penetrating effect superior to that of the penetrant liquid with long penetrating time. The method screens the penetrant with synergistic effect on the chemical for preventing and controlling chilo suppressalis by measuring the penetrant capability of the penetrant to the rice stem sheath, obtains the auxiliary agent with better penetrant capability to the rice leaf sheath and the heart leaf, and provides possibility for improving the preventing and controlling effect of the chemical on the chilo suppressalis and reducing the dosage of the chemical.
Description
Technical Field
The invention relates to the field of pesticides, and particularly relates to a screening method of a striped rice borer control pesticide penetrant and an application penetrant.
Background
Chilo suppressalis Chlio suppersalis (walker) is an important pest on rice and is harmful to larvae boring stalks to take food. At present, chemical control is an important measure for controlling chilo suppressalis, but pests, the pesticide liquid of which cannot permeate into rice stalks, are harmful, so that pesticide application is wasted and the control effect is low. The prior art generally improves the efficacy of the drug by increasing the attachment of the drug to the plant surface, but this method has no effect on boring chilo suppressalis larvae. Therefore, if a certain auxiliary agent can be added into the control agent, the auxiliary agent can improve the dosage of the chemical agent entering the habitat parts (in the leaf sheath and the heart leaf) of the chilo suppressalis larvae, so that the exposure chance of the chemical agent to the chilo suppressalis larvae can be increased, the dosage of the chemical agent to the chilo suppressalis larvae is improved, and the chemical agent can exert better control effect. However, the variety of the auxiliary agents is various, the cost is too high through a complex or direct prevention and control experiment, how to adopt a simple screening method to screen the auxiliary agents, and the screening result has reliable correlation with a real result is a key problem to be solved.
Disclosure of Invention
The invention aims to establish a screening method of a penetrant with a synergistic effect on a chemical for preventing and treating chilo suppressalis. The method screens the penetrant with synergistic effect on the chemical for preventing and controlling chilo suppressalis by measuring the penetrant capability of the penetrant to the rice stem sheath, obtains the auxiliary agent with better penetrant capability to the rice leaf sheath and the heart leaf, and provides possibility for improving the preventing and controlling effect of the chemical on the chilo suppressalis and reducing the dosage of the chemical.
The purpose of the invention can be realized by the following technical scheme: a method for screening the penetrant for preventing and controlling striped rice borer includes such steps as adding colouring agent to the liquid of different penetrants with same concentration, and measuring the time for each penetrant to penetrate through the rice stem with a certain length.
The invention also provides a more specific screening method of the striped rice borer control drug penetrant, which comprises the following steps:
(1) taking rice stems with specific length, trimming rice leaves, only keeping core and leaf parts of the rice stems, wrapping a circle of water absorbing material in the middle of an outer ring of the rice stems, and placing the base parts of the rice stems under the liquid level of water;
(2) adding coloring agent into different penetrant liquids with the same concentration, placing the capillary end of a capillary gun head connected with a capillary hose at the bottom of the penetrant liquid, placing the nozzle of the capillary gun at or above the central leaf of the rice stem, adjusting the height of the penetrant liquid to be higher than that of the capillary gun head, and enabling the penetrant liquid to automatically drop to the central leaf of the rice stem from the nozzle of the capillary gun and infiltrate downwards along the inside of the rice stem;
(3) the time required for the coloring penetrant to seep out from the rice stem base incision from the beginning of dripping to the rice heart leaf was observed and recorded, wherein the penetrant liquid having a short permeation time was more effective in permeation than the penetrant liquid having a long permeation time.
The colorant of the present invention is a colorant that is visible to the naked eye and does not affect a penetrant, and from the viewpoint of cost reduction and a combined effect, the present invention is preferably an ink, and more preferably a red ink for easy observation. The final concentration of the colorant can be adjusted for different colorants, preferably with the lowest volume fraction that can be clearly seen, typically in the range of 0.5% to 2.0%, more preferably 1.0% in the case of red inks.
The concentration of the penetrant liquid is preferably 0.6-1.0%, more preferably 0.8%, and the correlation between the measured result and the actual result is better.
The specific length of the invention is preferably 8-15 cm, preferably 10cm, and the performance of each penetrating agent can be effectively distinguished in the range.
The water absorbing material of the present invention may be a common water absorbing material which is convenient to fix and is used for preventing the liquid medicine from flowing down from the outside, such as paper towels, cotton, etc., and the paper towels are preferred.
The invention also provides a chilo suppressalis control medicament screened by the method, which comprises an effective amount of an active medicament and a penetrant, wherein the penetrant is selected from a rapid penetrant T70, a wetting agent GY-WS10, a super penetrant JFC-E or a penetrant GY-W07.
Penetrant the penetrant of the present invention is selected to have a good penetration rate to rice stems according to the penetration time. The invention verifies the synergistic capability of the permeation aid to chemical agents and the correlation between the synergistic effect of the permeation aid to the agents and the infiltration rate of the permeation aid to the agents in rice stem sheaths by utilizing an indoor bioassay experiment, and finally verifies the synergistic effect of the effective permeation aid screened by utilizing the method to the chemical agents again by utilizing a field plot experiment, thereby verifying that the permeation aid screened by utilizing the rice stem permeation rate method to screen the permeation aid for the synergism of the pesticide for preventing and controlling rice stem borers is feasible and effective. The method is simple and easy to implement, is convenient to operate, and has important significance for improving the control effect of the pesticide in the chilo suppressalis.
Penetrant
Drawings
FIG. 1 is a schematic view of a device for measuring the permeability of a penetrant to rice stalks.
Detailed Description
The following examples are given to facilitate a better understanding of the invention, but do not limit the invention. The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples were purchased from a conventional biochemical reagent store unless otherwise specified.
Example 1 screening of penetration aid by Rice straw penetration Rate method
1. Formulating pigmented penetrant liquids
The coloring liquid is prepared by ultrapure water from red ink and penetrant to be selected, the final concentration of the red ink is 1%, and the final concentrations of different penetrants are all 0.8% (most penetrants are recommended to use). An appropriate amount of the prepared coloring penetrant liquid is taken into a 10ml centrifuge tube for standby.
2. Installation measuring device
Firstly, taking rice seedlings cultured for about 10 days, cutting rice stalks with the length of 10cm, trimming rice leaves, only keeping heart and leaf parts, wrapping a circle of paper towel (preventing coloring liquid from flowing down along the outer part of the stalks) at the middle parts of the outer rings of the stalks, and placing the basal parts of the stalks below the liquid level of ultrapure water.
Then, the prepared coloring penetrant liquid is placed on a centrifuge tube frame, one end of a capillary hose is placed at the bottom of the centrifuge tube frame, the other end of the hose sucks the liquid medicine by an ear washing ball, the liquid medicine flows down along the tube and is quickly connected with a tip of the capillary tip, and the liquid level and the height of the tip of the capillary tip are adjusted, so that the liquid medicine can slowly flow out from the tip of the capillary tip. The rice stem is placed in the capillary gun head opening, the coloring liquid medicine can automatically drop into rice heart leaves (bell mouths) by the siphon principle, the coloring liquid medicine seeps downwards along the inside of the stem, and the paper towel wrapped outside the stem can prevent the liquid medicine from flowing downwards from the outer side (see figure 1).
Finally, the time required for the coloring penetrant to bleed out from the rice stalk base cut, starting from dripping to the rice heart leaves, was observed and recorded (three replicates of each penetrant). According to the time length of different penetration assisting agents penetrating from the inside of 10cm rice stems, 15 tested penetration agents can be divided into 4 types, the first type has strong penetration capacity on rice stem sheaths, the penetration time is less than 10min, and the penetration assisting agents are quick penetration agents T70, wetting agents GY-WS10, super-strong penetration agents JFC-E and penetration agents GY-W07; the second type has strong permeability to rice stem sheath, the exudation time is between 10min and 20min, and the second type is penetrant JFC-1, low-foam penetrant SF, fast penetrant JFC-M, organosilicon synergist GY-S903, alkali-resistant penetrant AEP and penetrant JFC; the third type has general permeability to rice stem sheath, the seepage time is about 1 hour, and the third type is alkali-resistant penetrant OEP-70; the fourth type has poor permeability to rice stem sheath, the exudation time is more than 8 hours, and the penetration agents are spray synergist GY-T1602, spray synergist GY-Spry, penetration agent JFC-2 and penetration agent OE-35. See table 1 for details.
TABLE 1 statistics of the efflux time of a 10cm vertical infiltration of a 0.8% concentration solution of different penetrants
3. Laboratory bioassay experiment
6 (quick penetrant T70, wetting agent GY-WS10, quick penetrant JFC-M, low foam penetrant SF, organic silicon synergist GY-S903 and alkali-resistant penetrant OEP-70) selected by the rice stem penetration method belong to strong, strong and general three types of penetration aids, and are respectively added with the insecticidal single abamectin pesticide for compounding and combination. The final concentration of each penetrant is 0.8%, the concentration of the monosultap and abamectin compound is 114.5mg/L (110mg/L monosultap +4.5mg/L abamectin), and the pesticide compound without penetrant is used as a control group. And (3) respectively spraying the 7 kinds of liquid medicines to the chilo suppressalis primary-hatched larvae, treating 30 larvae with each liquid medicine, repeating the treatment for three times, and observing and counting the death number of the larvae after 72 hours. The results show that: the lethality rates of 6 different auxiliary penetrant added medicaments to Chilo suppressalis larvae are respectively 97%, 94%, 93%, 91%, 96% and 67%, and compared with the lethality rate (44%) of a control group medicament without the auxiliary penetrant to the Chilo suppressalis, the lethality rates are obviously improved; meanwhile, the infiltration rate of 0.8 percent of the penetration assisting agent in the rice stem has obvious positive correlation with the synergistic effect on the medicament, and the correlation coefficient is 0.94. The infiltration rate of the penetrant in the rice stem is an important factor influencing the synergistic capability of the penetrant on medicaments, and the fact that the penetrant with the synergistic effect on the prevention and the treatment of chilo suppressalis is screened by the rice stem infiltration method is reliable.
4. Plot experiment
Respectively adding 200 ml/mu of 30% of hydramine-triazophos EC into a rapid penetrant T70, a wetting agent GY-WS10, a super-strong penetrant JFC-E and a penetrant GY-W07 respectively, wherein each penetrant isThe final concentration of the penetrant is 0.8%, 30% of water amine triazophos EC 200 ml/mu without penetrant is used as a control group, and clear water spray is used as a blank control group. The 6 kinds of liquid medicines are sprayed at the terminal stage of rice booting (at the time of chilo suppressalis egg hatching peak period), the area of each treated cell is 90 square meters, and the steps are repeated three times. A field survey was conducted 23 days after the application of the drug, and rice in the center area of the plot was used as a survey subject (survey area 4 m.times.13 m-42 m)2) And (4) surveying and counting all the white ear plants in the white ear, and calculating the ear protection effect.
The results show that: after the 30% of the aqueous amine triazophos EC 200 ml/mu is mixed with the rapid penetrant T70, the wetting agent GY-WS10, the super-strong penetrant JFC-E or the penetrant GY-W07, the control effect on chilo suppressalis is obviously improved, the ear protection effect is between 58.4% and 70.1%, and the ear protection effect is obviously higher than that of the 30% of the aqueous amine triazophos EC 200 ml/mu (42.3%). The 4 penetration assisting agents are shown to have obvious synergistic effect on 30% of hydroamine triazophos EC, and specific results are shown in Table 2.
And field plot experiments prove that the auxiliary penetrant with synergistic effect on preventing and controlling chilo suppressalis is screened by a rice stem penetration method to be reliable.
In table 230%, the land control effect of the amitraz triazophos EC on Chilo suppressalis after being tank-mixed with different additives
The letters after the same column of data indicate significant differences from each other (P <0.05)
Claims (10)
1. A method for screening the penetrant for preventing and controlling striped rice borer features that the colouring agent is added to the liquid of different penetrants with same concentration, and the time for each penetrant to penetrate through the rice stem with a certain length is measured, where the penetrant with short penetrating time has higher penetrating effect than the penetrant with long penetrating time.
2. A method for screening a striped rice borer control drug penetrant is characterized by comprising the following steps:
(1) taking rice stems with specific length, trimming rice leaves, only keeping core and leaf parts of the rice stems, wrapping a circle of water absorbing material in the middle of an outer ring of the rice stems, and placing the base parts of the rice stems under the liquid level of water;
(2) adding coloring agent into different penetrant liquids with the same concentration, placing the capillary end of a capillary gun head connected with a capillary hose at the bottom of the penetrant liquid, placing the nozzle of the capillary gun at or above the central leaf of the rice stem, adjusting the height of the penetrant liquid to be higher than that of the capillary gun head, and enabling the penetrant liquid to automatically drop to the central leaf of the rice stem from the nozzle of the capillary gun and infiltrate downwards along the inside of the rice stem;
(3) the time required for the coloring penetrant to seep out from the rice stem base incision from the beginning of dripping to the rice heart leaf was observed and recorded, wherein the penetrant liquid having a short permeation time was more effective in permeation than the penetrant liquid having a long permeation time.
3. A method according to claim 1 or 2, wherein the colorant is an ink.
4. The method of claim 3 wherein said colorant is a red ink.
5. A method according to claim 1 or 2, wherein the volume fraction of the colourant is in the range 0.5% to 2.0%.
6. The method of claim 5, wherein the volume fraction is 1.0% when the colorant is a red ink.
7. A method according to claim 1 or 2, wherein the concentration of the osmotic agent liquid is between 0.6% and 1.0%, preferably 0.8%.
8. A method according to claim 1 or 2, wherein the specific length is 8-15 cm, preferably 10 cm.
9. The method of claim 2, wherein the water absorbent material is paper towel or cotton.
10. The chemical for preventing and controlling chilo suppressalis is characterized by comprising an effective amount of an active chemical and a penetrating agent, wherein the penetrating agent is selected from a rapid penetrating agent T70, a wetting agent GY-WS10, a super-strong penetrating agent JFC-E or a penetrating agent GY-W07.
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CN114894675A (en) * | 2022-05-06 | 2022-08-12 | 江苏省农业科学院 | Comparison method for penetration behavior of pesticide auxiliary agent on surface of plant leaf |
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CN114894675B (en) * | 2022-05-06 | 2024-04-19 | 江苏省农业科学院 | Comparison method of penetration behaviors of pesticide auxiliary agent on plant leaf surfaces |
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