CN110739058B - Mesenchymal stem cell management system and shaking device and method - Google Patents

Mesenchymal stem cell management system and shaking device and method Download PDF

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CN110739058B
CN110739058B CN201911131968.7A CN201911131968A CN110739058B CN 110739058 B CN110739058 B CN 110739058B CN 201911131968 A CN201911131968 A CN 201911131968A CN 110739058 B CN110739058 B CN 110739058B
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module
umbilical cord
clamping seat
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frame
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CN110739058A (en
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王少波
王雅茹
杜明
王国栋
刘刚
贾静辉
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Tangshan Shinow Technology Co ltd
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Abstract

The invention relates to a mesenchymal stem cell management system and a shaking device and method, comprising an acquisition table module, a receiving module, a separation module, a culture module, a user management module, an umbilical cord library module, a permission management module, a checking module, a quality evaluation module, a warehousing module, a cell module, a micro-experiment-free module and an inventory inquiry module; the inspection module comprises a cell inspection module and a micro-inspection-free module; the acquisition meter module is used for inputting the acquisition information of the umbilical cord; the receiving module records the receiving information of the umbilical cord library module and marks the abnormal information in the umbilical cord library module; the invention has reasonable design, compact structure and convenient use.

Description

Mesenchymal stem cell management system and shaking device and method
Technical Field
The invention relates to a mesenchymal stem cell management system, a shaking device and a shaking method.
Background
The mesenchymal stem cells are clinically applied to solve various blood system diseases, cardiovascular diseases, liver cirrhosis, nervous system diseases, knee joint meniscus partial excision injury repair, autoimmune diseases and the like, and have made a great breakthrough, thus saving the lives of more patients. In addition, mesenchymal stem cells have long-term development prospects in the aspects of nervous system repair and more. A relatively perfect stem cell full life cycle management information system is established to meet the requirements of stem cell collection, storage, retrieval, processing and statistical analysis, and the provision of information source service support for stem cell transformation medical research becomes a technical problem to be solved urgently.
Over time, umbilical cord inspection technology is continuously improved, inspection equipment is also continuously upgraded, and the existing working mode flow of an umbilical cord blood bank is unreasonable, so that supervision and business control are not facilitated; most of the data are recorded by paper, so that the data are inconvenient to inquire, the labor cost is high, the informatization management is not facilitated, a complete and clear business management system is urgently needed, the data can be recorded by laboratory staff conveniently, various reports can be exported by clinical application, and the data are in accordance with the national inspection standard. The existing mesenchymal stem cell business management system mainly comprises CRM+pretreatment management+sample library, so that the follow-up and service quality of clients cannot be improved, the cooperation between the market and a laboratory cannot be realized, and the cell storage management is disordered. The information input points in the prior art are lagged, the responsibility is unclear, part of the flow is long, the approval efficiency is low, the optimization processing is required, the flow is unreasonable, and supervision and business control are not facilitated; lack of sharing of information, underutilization of information resources: various business statistics depend on manual operation, and the due value of an information system is not exerted; repeating the following steps: and a large number of repeated works exist in each department, so that data are repeatedly recorded, and the consistency of the data is difficult to be ensured.
The mesenchymal stem cell batch culture equipment is a device for culturing the separated mesenchymal stem cells again so as to improve the yield of the mesenchymal stem cells and obtain more mesenchymal stem cells. However, the existing mesenchymal stem cell culture equipment is inconvenient to fix, the culture flask is easy to collide in the shaking process, so that the culture flask is damaged, and the shaking efficiency is low. In order to solve the above problems, although CN201821633939.1 is an innovative design of a mesenchymal stem cell batch culture apparatus based on the original mesenchymal stem cell batch culture apparatus, the structure is complex and the shaking efficiency is low.
Disclosure of Invention
The invention aims to provide a mesenchymal stem cell management system, a shaking device and a shaking method.
In order to solve the problems, the invention adopts the following technical scheme:
a mesenchymal stem cell management system comprises an acquisition table module, a receiving module, a separation module, a culture module, a user management module, an umbilical cord library module, a right management module, a checking module, a quality evaluation module, a warehousing module, a cell module, a micro-experiment-free module and an inventory inquiry module; the inspection module comprises a cell inspection module and a micro-inspection-free module;
the acquisition meter module is used for inputting the acquisition information of the umbilical cord;
the receiving module records the receiving information of the umbilical cord library module and marks the abnormal information in the umbilical cord library module;
the separation module records the result of separating the umbilical cord from the umbilical cord library module;
the culturing module is used for recording the result of culturing the umbilical cord of the umbilical cord library module;
the cell detection module comprises a bacteria detection module, a mycoplasma detection module and a flow detection module;
the bacteria detection module is used for detecting aerobic bacteria and anaerobic bacteria and fungi of cells of the umbilical cord library module;
the mycoplasma detection module is used for detecting mycoplasma in cells of the umbilical cord library module;
the flow detection module is used for detecting phenotype items of the umbilical cord library module;
the micro-experiment module is used for inquiring the umbilical cord micro-experiment result, the bacterial detection and the mycoplasma detection result of the umbilical cord library module;
the quality evaluation module monitors whether the umbilical cord receiving, separating, culturing and detecting links of the umbilical cord library module are finished and generates evaluation information;
the warehousing module records the warehoused umbilical cord of the umbilical cord library module;
the user management module is used for managing the umbilical cord library module by division, different management personnel are arranged on different modules, and when an operator is selected, the operator is searched according to the module name;
and the authority management module is used for setting the authority of the manager to log in the umbilical cord library module.
As a further improvement of the above technical scheme:
phenotypic items include CD73, CD90, CD105, CD45, CD34, CD11b, CD29, HLA-DR.
The umbilical cord library module is provided with a customer information management module, a contract auditing and project reserving module, a sample receiving and quality testing module, a cell intelligent warehouse-in and warehouse-out module and an information security module;
in the client information management module, client profiling and follow-up are carried out, client self-defined labels and screening search are carried out, and a contract service project is self-defined;
the contract auditing and project reserving module is used for executing sampling handover and submitting tasks to a laboratory;
the sample receiving and quality testing module is used for carrying out pretreatment informatization flow, carrying out sample task receiving and distribution, carrying out sample custom labeling, carrying out laboratory preparation archive recording and uploading, and carrying out sample quality testing registration and auditing;
the method comprises the steps of customizing a label, and establishing a visual cell bank interface and a custom cell bank model;
the cell intelligent warehouse-in and warehouse-out module is used for carrying out a cell warehouse-in and warehouse-out informatization process; warehousing, namely performing intelligent recommendation position warehousing, importing samples in batches for warehousing, printing labels, and distinguishing single tube IDs; a warehouse-out operation, multi-field retrieval is carried out, a storage path is automatically prompted, and codes are scanned and the warehouse-out operation is carried out;
the information security module is used for setting a high security level by adopting an Arian Mysql database;
and the acquisition table module is used for: and managing the information of the puerpera.
In the receiving, separating and culturing module, using unique bar code for each umbilical cord, and using bar code scanning gun to identify bar code;
the detection module is used for detecting the umbilical cord and comprises the steps of collecting and managing experimental data of a micro-immune laboratory and a cell laboratory;
the quality evaluation module is used for automatically evaluating multiple aspects of umbilical cord quality, storability and applicability through preset evaluation conditions on all key data in the acquisition, preparation and detection processes, recording abnormal information in the umbilical cord full-service process and issuing relevant quality notification; and control umbilical cord discard; when the quality problem is required to occur, the quality problem can be reported to the management layer through the intelligent terminal.
The system manager of the user management module can uniformly manage personnel authority, short message sending and function running conditions.
A mesenchymal stem cell temporary shaking device, which comprises a rotary chassis;
a rotating frame is rotatably arranged on the rotating chassis, a supporting frame is arranged on the rotating chassis, and a rotating driving head for driving the rotating frame to rotate is arranged on the supporting frame; the bottom array of the outer side wall of the driving rotating frame is radially distributed with hinged lower spring rods, the upper array of the outer side wall of the driving rotating frame is radially distributed with hinged upper spring rods, the hinged lower spring rods are connected with vertical auxiliary frames, the hinged upper spring rods are connected with upper auxiliary frames, and an intermediate auxiliary frame is connected between the vertical auxiliary frames and the upper auxiliary frames;
an upper rotating sleeve is arranged on the upper auxiliary frame, an upper U-shaped clamping seat is rotatably arranged on the upper rotating sleeve, a lower rotating sleeve is arranged on the vertical auxiliary frame, and the lower rotating sleeve is connected with a lower U-shaped clamping seat through a rotating shaft;
the outer side walls of test tubes for culturing umbilical cords are clamped in the upper U-shaped clamping seat and the lower U-shaped clamping seat;
the upper U-shaped clamping seat and the lower U-shaped clamping seat for clamping the same test tube are vertically staggered on the rotating frame; the test tube is arranged obliquely.
As a further improvement of the above technical scheme:
the lower end of the lower U-shaped clamping seat is connected with a lower bracket, and the lower end of the lower bracket is connected with a lower U-shaped bottom bracket; the lower U-shaped bottom bracket lifts the lower bottom of the test tube.
The method is applied to the umbilical cord culture process of a mesenchymal stem cell management system.
The mesenchymal stem cell management method comprises the following steps that firstly, donation records are carried out, and after the acquisition list is recorded, an umbilical cord receives the record;
step two, umbilical cord separation is carried out;
step three, umbilical cord culture is carried out;
and step four, warehousing the umbilical cord, and recording an inventory standing book.
As a further improvement of the above technical scheme:
in the second step, after the umbilical cord is separated, umbilical cord culture is carried out, umbilical cord warehousing is carried out, meanwhile, management personnel carry out flow inquiry and quality evaluation, and after the umbilical cord passes the auditing, isolated distribution is carried out; then, shifting the application, and discarding the application for delivery after checking;
in the third step, after umbilical cord culture, carrying out flow detection and auditing on a sample, and generating a quality notification when a disqualified item exists; then, generating an autologous quality notification;
in the third step, after umbilical cord culture, firstly, bacteria detection and auditing are carried out on the distributed samples, meanwhile, mother blood receiving is carried out, mother blood sample retention is carried out on one part, and primary screening detection is carried out on one part; then, when the primary screening detection finds positive, rechecking detection is carried out; secondly, carrying out confirmation detection on syphilis, HIV and positive; secondly, checking bacteria detection, preliminary screening detection, rechecking qualification and confirmation detection; thirdly, generating a micro-exemption conclusion after the auditing result is qualified, and generating a quality notification when a disqualified item exists; then, generating an autologous quality notification;
in the fourth step, the abnormal umbilical cord is isolated, firstly, shift isolation is carried out, and when a abandoned notice of a mass and management department is received; the discarded umbilical cord is then taken out of stock.
A mesenchymal stem cell shaking method, which is used for an umbilical cord culture process; firstly, adjusting the angles of an upper U-shaped clamping seat and a lower U-shaped clamping seat, clamping a test tube in the upper U-shaped clamping seat and the lower U-shaped clamping seat, and lifting the lower bottom of the test tube through a lower U-shaped bottom support; then, starting the rotary driving head, and rotating the rotary frame; secondly, the lower spring rod is started to rotate and the rotating speed is adjusted by the upper spring rod, and the test tube is rotated by utilizing centrifugal force, so that rotation and rotation tangential shaking in the test tube are realized.
The invention has reasonable design, low cost, firmness, durability, safety, reliability, simple operation, time and labor saving, fund saving, compact structure and convenient use.
Drawings
Fig. 1 is a schematic structural diagram of the management system of the present invention.
Fig. 2 is a schematic illustration of the flow of the present invention.
FIG. 3 is a schematic diagram of a process according to an embodiment of the present invention.
FIG. 4 is a schematic diagram of a process according to the second embodiment of the present invention.
Fig. 5 is a schematic diagram of a process of the present invention in a third embodiment.
Fig. 6 is a schematic structural view of the shaking device of the present invention.
Fig. 7 is a schematic structural view of a supporting frame of the shaking device of the present invention.
Wherein: 1. rotating the chassis; 2. a rotating frame; 3. a support frame; 4. rotating the drive head; 5. a lower spring rod is hinged; 6. a spring rod is hinged; 7. a vertical auxiliary frame; 8. a middle auxiliary frame; 9. an upper auxiliary frame; 10. an upper rotating sleeve; 11. a U-shaped clamping seat is arranged; 12. a lower rotating sleeve; 13. a lower U-shaped clamping seat; 14. a lower bracket; 15. a lower U-shaped bottom bracket.
Detailed Description
Referring to FIGS. 1 to 7, the mesenchymal stem cell shaking device of the present embodiment, as shown in FIGS. 6 to 7, comprises a rotary chassis 1; a rotary frame 2 is rotatably arranged on the rotary chassis 1, a support frame 3 is arranged on the rotary chassis 1, and a rotary driving head 4 for driving the rotary frame 2 to rotate is arranged on the support frame 3; the bottom array of the outer side wall of the driving rotating frame 2 is radially distributed with hinged lower spring rods 5, the upper array of the outer side wall of the driving rotating frame 2 is radially distributed with hinged upper spring rods 6, the hinged lower spring rods 5 are connected with vertical auxiliary frames 7, the hinged upper spring rods 6 are connected with upper auxiliary frames 9, and middle auxiliary frames 8 are connected between the vertical auxiliary frames 7 and the upper auxiliary frames 9;
an upper rotary sleeve 10 is arranged on the upper auxiliary frame 9, an upper U-shaped clamping seat 11 is rotatably arranged on the upper rotary sleeve 10, a lower rotary sleeve 12 is arranged on the vertical auxiliary frame 7, and the lower rotary sleeve 12 is connected with a lower U-shaped clamping seat 13 through a rotary shaft;
the outer side walls of test tubes for culturing umbilical cords are clamped in the upper U-shaped clamping seat 11 and the lower U-shaped clamping seat 13;
the upper U-shaped clamping seat 11 and the lower U-shaped clamping seat 13 for clamping the same test tube are vertically staggered on the rotating frame 2; the test tube is arranged obliquely.
The lower end of the lower U-shaped clamping seat 13 is connected with a lower bracket 14, and the lower end of the lower bracket 14 is connected with a lower U-shaped bottom bracket 15; the lower U-shaped bottom bracket 15 lifts the lower bottom of the test tube.
The method is applied to the umbilical cord culture process of a mesenchymal stem cell management system.
When the invention is used, the rotary chassis 1 is used as a support, the rotary frame 2 is rotated to realize driving, the support frame 3 is used for realizing auxiliary support, the rotary driving head 4 is used for realizing rotary driving, and through rotary shaking, the lower spring rod 5 is hinged, the upper spring rod 6 is hinged to extend out of a corresponding distance under the action of centrifugal force, the vertical auxiliary frame 7, the middle auxiliary frame 8 and the upper auxiliary frame 9 are connected in an auxiliary manner, the upper spring rod and the lower spring rod are guaranteed to rotate at the same speed at the same time, the upper rotary sleeve 10, the lower rotary sleeve 12 are rotatably supported, the upper U-shaped clamping seat 11 and the lower U-shaped clamping seat 13 are internally provided with buffer cushions, so that a test tube which revolves obliquely is rotated, the up-and-down shaking in the test tube is realized through rotating speed adjustment, and meanwhile, the radial movement is realized, the full shaking of the test tube is realized, the shaking effect is improved, and the bottom lifting is realized through the lower U-shaped bottom support 15.
The mesenchymal stem cell management method comprises the following steps that firstly, donation records are carried out, and after the acquisition list is recorded, an umbilical cord receives the record;
step two, umbilical cord separation is carried out;
step three, umbilical cord culture is carried out;
and step four, warehousing the umbilical cord, and recording an inventory standing book.
In the second step, after the umbilical cord is separated, umbilical cord culture is carried out, umbilical cord warehousing is carried out, meanwhile, management personnel carry out flow inquiry and quality evaluation, and after the umbilical cord passes the auditing, isolated distribution is carried out; then, shifting the application, and discarding the application for delivery after checking;
in the third step, after umbilical cord culture, carrying out flow detection and auditing on a sample, and generating a quality notification when a disqualified item exists; then, generating an autologous quality notification;
in the third step, after umbilical cord culture, firstly, bacteria detection and auditing are carried out on the distributed samples, meanwhile, mother blood receiving is carried out, mother blood sample retention is carried out on one part, and primary screening detection is carried out on one part; then, when the primary screening detection finds positive, rechecking detection is carried out; secondly, carrying out confirmation detection on syphilis, HIV and positive; secondly, checking bacteria detection, preliminary screening detection, rechecking qualification and confirmation detection; thirdly, generating a micro-exemption conclusion after the auditing result is qualified, and generating a quality notification when a disqualified item exists; then, generating an autologous quality notification;
in the fourth step, the abnormal umbilical cord is isolated, firstly, shift isolation is carried out, and when a abandoned notice of a mass and management department is received; the discarded umbilical cord is then taken out of stock.
The mesenchymal stem cell shaking method of the embodiment is used for an umbilical cord culture process; firstly, adjusting the angles of an upper U-shaped clamping seat 11 and a lower U-shaped clamping seat 13, clamping a test tube in the upper U-shaped clamping seat 11 and the lower U-shaped clamping seat 13, and lifting the lower bottom of the test tube through a lower U-shaped bottom bracket 15; then, the rotary driving head 4 is started, and the rotary frame 2 rotates; secondly, the hinged lower spring rod 5 and the hinged upper spring rod 6 are started to rotate and adjust the rotating speed, and the rotation of the test tube is realized by utilizing the centrifugal force, so that the rotation and the rotation tangential shaking in the test tube are realized.
1-5, the mesenchymal stem cell management system of the embodiment comprises an acquisition table module, a receiving module, a separation module, a culturing module, a user management module, an umbilical cord library module, a right management module, a checking module, a quality evaluation module, a warehousing module, a cell module, a micro-experiment-free module and an inventory query module; the inspection module comprises a cell inspection module and a micro-inspection-free module;
the acquisition meter module is used for inputting the acquisition information of the umbilical cord;
the receiving module records the receiving information of the umbilical cord library module and marks the abnormal information in the umbilical cord library module;
the separation module records the result of separating the umbilical cord from the umbilical cord library module;
the culturing module is used for recording the result of culturing the umbilical cord of the umbilical cord library module;
the cell detection module comprises a bacteria detection module, a mycoplasma detection module and a flow detection module;
the bacteria detection module is used for detecting aerobic bacteria and anaerobic bacteria and fungi of cells of the umbilical cord library module;
the mycoplasma detection module is used for detecting mycoplasma in cells of the umbilical cord library module;
the flow detection module is used for detecting phenotype items of the umbilical cord library module; phenotypic items include CD73, CD90, CD105, CD45, CD34, CD11b, CD29, HLA-DR;
the micro-experiment module is used for inquiring the umbilical cord micro-experiment result, the bacterial detection and the mycoplasma detection result of the umbilical cord library module;
the quality evaluation module monitors whether the umbilical cord receiving, separating, culturing and detecting links of the umbilical cord library module are finished and generates evaluation information;
the warehousing module records the warehoused umbilical cord of the umbilical cord library module;
the user management module is used for managing the umbilical cord library module by division, different management personnel are arranged on different modules, and when an operator is selected, the operator is searched according to the module name;
and the authority management module is used for setting the authority of the manager to log in the umbilical cord library module.
The umbilical cord library module is provided with a customer information management module, a contract auditing and project reserving module, a sample receiving and quality testing module, a cell intelligent warehouse-in and warehouse-out module and an information security module;
in the client information management module, client profiling and follow-up are carried out, client self-defined labels and screening search are carried out, and a contract service project is self-defined;
the contract auditing and project reserving module is used for executing sampling handover and submitting tasks to a laboratory;
the sample receiving and quality testing module is used for carrying out pretreatment informatization flow, carrying out sample task receiving and distribution, carrying out sample custom labeling, carrying out laboratory preparation archive recording and uploading, and carrying out sample quality testing registration and auditing;
the method comprises the steps of customizing a label, and establishing a visual cell bank interface and a custom cell bank model;
the cell intelligent warehouse-in and warehouse-out module is used for carrying out a cell warehouse-in and warehouse-out informatization process; warehousing, namely performing intelligent recommendation position warehousing, importing samples in batches for warehousing, printing labels, and distinguishing single tube IDs; a warehouse-out operation, multi-field retrieval is carried out, a storage path is automatically prompted, and codes are scanned and the warehouse-out operation is carried out;
the information security module is used for setting a high security level by adopting an Arian Mysql database;
and the acquisition table module is used for: and managing the information of the puerpera.
In the receiving, separating and culturing module, each umbilical cord is identified using a unique barcode, which is identified using a barcode scanning gun.
The umbilical cord during the preparation process provides a checking mechanism to ensure the correctness of the bar code when it is filled into a new bag. To ensure that the preparation process is as automated as possible, the system provides a variety of interfaces associated with the preparation equipment. To improve the stability and recovery rate of cell separation, the safety of the umbilical cord blood preparation process is ensured. The system can generate early warning information according to the abnormal condition of the experimental data.
The detection module is used for detecting the umbilical cord and comprises the steps of collecting and managing experimental data of a micro-immune laboratory and a cell laboratory;
the detection instruments used by different detection projects are different, the user management module system supports manual data input, and also supports acquisition of test data through an automatic acquisition interface, so that manual links are reduced, workload is reduced, and accuracy is improved.
The quality evaluation module is used for automatically evaluating multiple aspects of umbilical cord quality, storability and applicability through preset evaluation conditions on all key data in the acquisition, preparation and detection processes, recording abnormal information in the umbilical cord full-service process and issuing relevant quality notification; and control umbilical cord discard; when the quality problem is required to occur, the quality problem can be reported to the management layer through the intelligent terminal.
The authority management module enables a system manager of the user management module to uniformly manage personnel authority, short message sending and function running conditions;
the collaboration capacity of each department is greatly improved; the quality control department can monitor the whole life cycle of the business process.

Claims (3)

1. The mesenchymal stem cell temporary shaking device is characterized by comprising a rotary chassis (1);
a rotating frame (2) is rotatably arranged on the rotating chassis (1), a supporting frame (3) is arranged on the rotating chassis (1), and a rotating driving head (4) for driving the rotating frame (2) to rotate is arranged on the supporting frame (3); the bottom array of the outer side wall of the driving rotating frame (2) is radially distributed with hinged lower spring rods (5), the upper array of the outer side wall of the driving rotating frame (2) is radially distributed with hinged upper spring rods (6), the hinged lower spring rods (5) are connected with vertical auxiliary frames (7), the hinged upper spring rods (6) are connected with upper auxiliary frames (9), and an intermediate auxiliary frame (8) is connected between the vertical auxiliary frames (7) and the upper auxiliary frames (9);
an upper rotary sleeve (10) is arranged on the upper auxiliary frame (9), an upper U-shaped clamping seat (11) is rotatably arranged on the upper rotary sleeve (10), a lower rotary sleeve (12) is arranged on the vertical auxiliary frame (7), and the lower U-shaped clamping seat (13) is connected with the lower rotary sleeve (12) through a rotary shaft;
the outer side walls of test tubes for culturing umbilical cords are clamped in the upper U-shaped clamping seat (11) and the lower U-shaped clamping seat (13);
an upper U-shaped clamping seat (11) and a lower U-shaped clamping seat (13) for clamping the same test tube are vertically staggered on the rotating frame (2); the test tube is arranged obliquely.
2. The mesenchymal stem cell temporary shaking device according to claim 1 is characterized in that the lower end of the lower U-shaped clamping seat (13) is connected with a lower bracket (14), and the lower end of the lower bracket (14) is connected with a lower U-shaped bottom bracket (15); the lower U-shaped bottom bracket (15) lifts the lower bottom of the test tube.
3. The device of claim 2, wherein the device is used in umbilical cord culture process of mesenchymal stem cell management system.
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