CN110731919A - Application of Mirabilis jalapa extracts and skin external preparation containing the same - Google Patents
Application of Mirabilis jalapa extracts and skin external preparation containing the same Download PDFInfo
- Publication number
- CN110731919A CN110731919A CN201810804107.XA CN201810804107A CN110731919A CN 110731919 A CN110731919 A CN 110731919A CN 201810804107 A CN201810804107 A CN 201810804107A CN 110731919 A CN110731919 A CN 110731919A
- Authority
- CN
- China
- Prior art keywords
- mirabilis jalapa
- skin
- extract
- percentage
- mirabilis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000022198 Mirabilis jalapa Species 0.000 title claims abstract description 146
- 235000009053 Mirabilis jalapa Nutrition 0.000 title claims abstract description 146
- 239000000284 extract Substances 0.000 title claims abstract description 131
- 238000002360 preparation method Methods 0.000 title claims description 71
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 33
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 32
- 239000003112 inhibitor Substances 0.000 claims abstract description 20
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 19
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 16
- 230000007062 hydrolysis Effects 0.000 claims abstract description 14
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 14
- 210000004177 elastic tissue Anatomy 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 39
- 108010035532 Collagen Proteins 0.000 claims description 30
- 102000008186 Collagen Human genes 0.000 claims description 30
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 30
- 229920001436 collagen Polymers 0.000 claims description 30
- 230000032683 aging Effects 0.000 claims description 28
- 238000000605 extraction Methods 0.000 claims description 26
- 108090000654 Bone morphogenetic protein 1 Proteins 0.000 claims description 21
- 239000008367 deionised water Substances 0.000 claims description 18
- 229910021641 deionized water Inorganic materials 0.000 claims description 18
- 239000003755 preservative agent Substances 0.000 claims description 18
- 238000010438 heat treatment Methods 0.000 claims description 17
- 230000002335 preservative effect Effects 0.000 claims description 17
- 239000004480 active ingredient Substances 0.000 claims description 16
- YPFDHNVEDLHUCE-UHFFFAOYSA-N propane-1,3-diol Chemical group OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 claims description 16
- 239000002904 solvent Substances 0.000 claims description 16
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 claims description 15
- 230000003078 antioxidant effect Effects 0.000 claims description 14
- 230000009759 skin aging Effects 0.000 claims description 14
- SNPLKNRPJHDVJA-ZETCQYMHSA-N D-panthenol Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCCO SNPLKNRPJHDVJA-ZETCQYMHSA-N 0.000 claims description 12
- 238000004587 chromatography analysis Methods 0.000 claims description 12
- 235000011187 glycerol Nutrition 0.000 claims description 12
- 229940101267 panthenol Drugs 0.000 claims description 12
- 235000020957 pantothenol Nutrition 0.000 claims description 12
- 239000011619 pantothenol Substances 0.000 claims description 12
- 239000000839 emulsion Substances 0.000 claims description 11
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical group O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 11
- 230000008099 melanin synthesis Effects 0.000 claims description 11
- 239000003921 oil Substances 0.000 claims description 11
- 102000012422 Collagen Type I Human genes 0.000 claims description 10
- 108010022452 Collagen Type I Proteins 0.000 claims description 10
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 10
- 230000003712 anti-aging effect Effects 0.000 claims description 10
- 239000011347 resin Substances 0.000 claims description 10
- 229920005989 resin Polymers 0.000 claims description 10
- 239000000230 xanthan gum Substances 0.000 claims description 10
- 229920001285 xanthan gum Polymers 0.000 claims description 10
- 229940082509 xanthan gum Drugs 0.000 claims description 10
- -1 polyethylene Polymers 0.000 claims description 9
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 claims description 9
- 235000010493 xanthan gum Nutrition 0.000 claims description 9
- BANXPJUEBPWEOT-UHFFFAOYSA-N 2-methyl-Pentadecane Chemical compound CCCCCCCCCCCCCC(C)C BANXPJUEBPWEOT-UHFFFAOYSA-N 0.000 claims description 8
- BIVBRWYINDPWKA-VLQRKCJKSA-L Glycyrrhizinate dipotassium Chemical compound [K+].[K+].O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@H]1CC[C@]2(C)[C@H]3C(=O)C=C4[C@@H]5C[C@](C)(CC[C@@]5(CC[C@@]4(C)[C@]3(C)CC[C@H]2C1(C)C)C)C(O)=O)C([O-])=O)[C@@H]1O[C@H](C([O-])=O)[C@@H](O)[C@H](O)[C@H]1O BIVBRWYINDPWKA-VLQRKCJKSA-L 0.000 claims description 8
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 claims description 8
- 229940101029 dipotassium glycyrrhizinate Drugs 0.000 claims description 8
- 229960003415 propylparaben Drugs 0.000 claims description 8
- 239000004094 surface-active agent Substances 0.000 claims description 8
- 239000003995 emulsifying agent Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 7
- 238000001179 sorption measurement Methods 0.000 claims description 7
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 claims description 6
- 239000013543 active substance Substances 0.000 claims description 6
- 229940082500 cetostearyl alcohol Drugs 0.000 claims description 6
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 6
- 239000003480 eluent Substances 0.000 claims description 6
- 229940093629 isopropyl isostearate Drugs 0.000 claims description 6
- 239000002480 mineral oil Substances 0.000 claims description 6
- 235000010446 mineral oil Nutrition 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- GLDOVTGHNKAZLK-UHFFFAOYSA-N octadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCO GLDOVTGHNKAZLK-UHFFFAOYSA-N 0.000 claims description 6
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 6
- 229920005862 polyol Polymers 0.000 claims description 6
- 150000003077 polyols Chemical class 0.000 claims description 6
- NEOZOXKVMDBOSG-UHFFFAOYSA-N propan-2-yl 16-methylheptadecanoate Chemical compound CC(C)CCCCCCCCCCCCCCC(=O)OC(C)C NEOZOXKVMDBOSG-UHFFFAOYSA-N 0.000 claims description 6
- OULAJFUGPPVRBK-UHFFFAOYSA-N tetratriacontyl alcohol Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCO OULAJFUGPPVRBK-UHFFFAOYSA-N 0.000 claims description 6
- 229940042585 tocopherol acetate Drugs 0.000 claims description 6
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 claims description 5
- 239000006071 cream Substances 0.000 claims description 5
- 238000010828 elution Methods 0.000 claims description 5
- 239000003205 fragrance Substances 0.000 claims description 5
- 229960005323 phenoxyethanol Drugs 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims description 4
- 229940043268 2,2,4,4,6,8,8-heptamethylnonane Drugs 0.000 claims description 4
- OMIGHNLMNHATMP-UHFFFAOYSA-N 2-hydroxyethyl prop-2-enoate Chemical compound OCCOC(=O)C=C OMIGHNLMNHATMP-UHFFFAOYSA-N 0.000 claims description 4
- 239000004925 Acrylic resin Substances 0.000 claims description 4
- 229920000178 Acrylic resin Polymers 0.000 claims description 4
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 claims description 4
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 4
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 4
- 229960000458 allantoin Drugs 0.000 claims description 4
- 229920001577 copolymer Polymers 0.000 claims description 4
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims description 4
- KUVMKLCGXIYSNH-UHFFFAOYSA-N isopentadecane Natural products CCCCCCCCCCCCC(C)C KUVMKLCGXIYSNH-UHFFFAOYSA-N 0.000 claims description 4
- 238000002386 leaching Methods 0.000 claims description 4
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 claims description 4
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 claims description 4
- 229960002216 methylparaben Drugs 0.000 claims description 4
- 229940049964 oleate Drugs 0.000 claims description 4
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims description 4
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 3
- 208000002197 Ehlers-Danlos syndrome Diseases 0.000 claims description 3
- 229940122858 Elastase inhibitor Drugs 0.000 claims description 3
- 229940123457 Free radical scavenger Drugs 0.000 claims description 3
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 3
- 229960003237 betaine Drugs 0.000 claims description 3
- 239000003602 elastase inhibitor Substances 0.000 claims description 3
- 239000006210 lotion Substances 0.000 claims description 3
- 239000002304 perfume Substances 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 239000002516 radical scavenger Substances 0.000 claims description 3
- 230000002087 whitening effect Effects 0.000 claims description 3
- LXAHHHIGZXPRKQ-UHFFFAOYSA-N 5-fluoro-2-methylpyridine Chemical compound CC1=CC=C(F)C=N1 LXAHHHIGZXPRKQ-UHFFFAOYSA-N 0.000 claims description 2
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 2
- 101710147108 Tyrosinase inhibitor Proteins 0.000 claims description 2
- 239000004359 castor oil Substances 0.000 claims description 2
- 235000019438 castor oil Nutrition 0.000 claims description 2
- SZXQTJUDPRGNJN-UHFFFAOYSA-N dipropylene glycol Chemical compound OCCCOCCCO SZXQTJUDPRGNJN-UHFFFAOYSA-N 0.000 claims description 2
- 239000000945 filler Substances 0.000 claims description 2
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 claims description 2
- WCVRQHFDJLLWFE-UHFFFAOYSA-N pentane-1,2-diol Chemical compound CCCC(O)CO WCVRQHFDJLLWFE-UHFFFAOYSA-N 0.000 claims description 2
- 239000000049 pigment Substances 0.000 claims description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 2
- 229940068977 polysorbate 20 Drugs 0.000 claims description 2
- 239000012535 impurity Substances 0.000 claims 4
- 102100028728 Bone morphogenetic protein 1 Human genes 0.000 claims 3
- 238000000746 purification Methods 0.000 claims 2
- 235000010254 Jasminum officinale Nutrition 0.000 claims 1
- 240000005385 Jasminum sambac Species 0.000 claims 1
- 229920002334 Spandex Polymers 0.000 claims 1
- 235000010232 propyl p-hydroxybenzoate Nutrition 0.000 claims 1
- 239000004405 propyl p-hydroxybenzoate Substances 0.000 claims 1
- 229940127408 Melanin Synthesis Inhibitors Drugs 0.000 abstract description 2
- 210000003491 skin Anatomy 0.000 description 71
- 239000000243 solution Substances 0.000 description 30
- 230000000694 effects Effects 0.000 description 28
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 25
- 102000004152 Bone morphogenetic protein 1 Human genes 0.000 description 18
- 238000002835 absorbance Methods 0.000 description 15
- 239000002537 cosmetic Substances 0.000 description 14
- 210000002950 fibroblast Anatomy 0.000 description 13
- 102000003425 Tyrosinase Human genes 0.000 description 11
- 108060008724 Tyrosinase Proteins 0.000 description 11
- 239000000047 product Substances 0.000 description 11
- 102000016387 Pancreatic elastase Human genes 0.000 description 10
- 108010067372 Pancreatic elastase Proteins 0.000 description 10
- 235000006708 antioxidants Nutrition 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 9
- 230000005764 inhibitory process Effects 0.000 description 9
- 150000003254 radicals Chemical class 0.000 description 9
- 239000000523 sample Substances 0.000 description 9
- 239000012488 sample solution Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 7
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- OCZVHBZNPVABKX-UHFFFAOYSA-N 1,1-diphenyl-2-(2,4,6-trinitrophenyl)hydrazine;ethanol Chemical compound CCO.[O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1NN(C=1C=CC=CC=1)C1=CC=CC=C1 OCZVHBZNPVABKX-UHFFFAOYSA-N 0.000 description 6
- 230000002401 inhibitory effect Effects 0.000 description 6
- 210000004207 dermis Anatomy 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 102000016942 Elastin Human genes 0.000 description 4
- 108010014258 Elastin Proteins 0.000 description 4
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 4
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 4
- 235000010323 ascorbic acid Nutrition 0.000 description 4
- 229960005070 ascorbic acid Drugs 0.000 description 4
- 239000011668 ascorbic acid Substances 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 230000002500 effect on skin Effects 0.000 description 4
- 229920002549 elastin Polymers 0.000 description 4
- 210000002744 extracellular matrix Anatomy 0.000 description 4
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 239000012088 reference solution Substances 0.000 description 4
- 239000012086 standard solution Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000037303 wrinkles Effects 0.000 description 4
- 241000219470 Mirabilis Species 0.000 description 3
- 206010051246 Photodermatosis Diseases 0.000 description 3
- ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 description 3
- 239000003463 adsorbent Substances 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 238000002242 deionisation method Methods 0.000 description 3
- 230000007760 free radical scavenging Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 230000008845 photoaging Effects 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- IJWCGVPEDDQUDE-YGJAXBLXSA-N (2s)-2-[[(1s)-2-[[(2s)-5-amino-1,5-dioxo-1-[[(2s)-1-oxopropan-2-yl]amino]pentan-2-yl]amino]-1-[(6s)-2-amino-1,4,5,6-tetrahydropyrimidin-6-yl]-2-oxoethyl]carbamoylamino]-4-methylpentanoic acid Chemical compound O=C[C@H](C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)N[C@@H](CC(C)C)C(O)=O)[C@@H]1CCN=C(N)N1 IJWCGVPEDDQUDE-YGJAXBLXSA-N 0.000 description 2
- IJWCGVPEDDQUDE-UHFFFAOYSA-N Elastatinal Natural products O=CC(C)NC(=O)C(CCC(N)=O)NC(=O)C(NC(=O)NC(CC(C)C)C(O)=O)C1CCN=C(N)N1 IJWCGVPEDDQUDE-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 239000004354 Hydroxyethyl cellulose Substances 0.000 description 2
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 description 2
- 102000003820 Lipoxygenases Human genes 0.000 description 2
- 108090000128 Lipoxygenases Proteins 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- 208000012641 Pigmentation disease Diseases 0.000 description 2
- 108010050808 Procollagen Proteins 0.000 description 2
- 230000002292 Radical scavenging effect Effects 0.000 description 2
- 102000001732 Small Leucine-Rich Proteoglycans Human genes 0.000 description 2
- 108010040068 Small Leucine-Rich Proteoglycans Proteins 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 229960000271 arbutin Drugs 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000012490 blank solution Substances 0.000 description 2
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000000287 crude extract Substances 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 108010039262 elastatinal Proteins 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- BJRNKVDFDLYUGJ-UHFFFAOYSA-N p-hydroxyphenyl beta-D-alloside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-UHFFFAOYSA-N 0.000 description 2
- 150000002989 phenols Chemical class 0.000 description 2
- 230000019612 pigmentation Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 150000005846 sugar alcohols Polymers 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 1
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- 241000195940 Bryophyta Species 0.000 description 1
- 206010007247 Carbuncle Diseases 0.000 description 1
- AHMIDUVKSGCHAU-UHFFFAOYSA-N Dopaquinone Natural products OC(=O)C(N)CC1=CC(=O)C(=O)C=C1 AHMIDUVKSGCHAU-UHFFFAOYSA-N 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 206010014970 Ephelides Diseases 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 244000061408 Eugenia caryophyllata Species 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 206010023232 Joint swelling Diseases 0.000 description 1
- 208000001126 Keratosis Diseases 0.000 description 1
- AHMIDUVKSGCHAU-LURJTMIESA-N L-dopaquinone Chemical compound [O-]C(=O)[C@@H]([NH3+])CC1=CC(=O)C(=O)C=C1 AHMIDUVKSGCHAU-LURJTMIESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 206010027145 Melanocytic naevus Diseases 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- 208000007256 Nevus Diseases 0.000 description 1
- 241000192656 Nostoc Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 241000245063 Primula Species 0.000 description 1
- 235000000497 Primula Nutrition 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000123725 Sophora tonkinensis Species 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 235000016639 Syzygium aromaticum Nutrition 0.000 description 1
- 206010047141 Vasodilatation Diseases 0.000 description 1
- 241000589634 Xanthomonas Species 0.000 description 1
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N benzo-alpha-pyrone Natural products C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000005200 bud stage Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229940097217 cardiac glycoside Drugs 0.000 description 1
- 239000002368 cardiac glycoside Substances 0.000 description 1
- 230000001925 catabolic effect Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000008341 cosmetic lotion Substances 0.000 description 1
- 235000001671 coumarin Nutrition 0.000 description 1
- 150000004775 coumarins Chemical class 0.000 description 1
- 229920006037 cross link polymer Polymers 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 230000014155 detection of activity Effects 0.000 description 1
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 1
- 208000028659 discharge Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 230000002849 elastaseinhibitory effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000012259 ether extract Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000004761 fibrosis Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229940075529 glyceryl stearate Drugs 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229940093915 gynecological organic acid Drugs 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 150000002596 lactones Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 210000002752 melanocyte Anatomy 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 235000011929 mousse Nutrition 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 239000007764 o/w emulsion Substances 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 229940100460 peg-100 stearate Drugs 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 229930002534 steroid glycoside Natural products 0.000 description 1
- 150000008143 steroidal glycosides Chemical class 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 230000024883 vasodilation Effects 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 239000007762 w/o emulsion Substances 0.000 description 1
- 238000004078 waterproofing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
Abstract
The invention provides application of Mirabilis jalapa extracts and a skin external agent containing the same, wherein the application specifically comprises application of or more of Mirabilis jalapa extracts in preparing antioxidants, melanin synthesis inhibitors, elastic fiber hydrolysis inhibitors and anti-skin-aging protein expression up-regulating agents.
Description
Technical Field
The invention relates to application of Mirabilis jalapa extracts and a skin external preparation containing the same.
Background
With the continuous development and the gradual maturity of the skin care product market, more and more brands emerge, and the requirement of consumers for the efficacy of the skin care product is expected to see the practical and effective skin care effect, so the research level of the efficacy mechanism of the active substances of the skin care product needs to be improved, the explanation of the efficacy mechanism becomes deeper and deeper, the simple description of the images cannot be realized, the advanced biotechnology needs to be fused, the discovery of the skin biology is deepened continuously with the continuous development of the cytobiology technical level, and more targets and regulation mechanisms related to the functional structure of the skin are discovered.
The skin is the largest organ of the human body, covers the whole body, and mainly plays a role in protecting the body, removing sweat, sensing cold and heat, pressure and the like, so that various tissues and organs in the body are prevented from being invaded by physical, mechanical, chemical and pathogenic microorganisms.
Skin aging is classified into endogenous aging and exogenous aging. Intrinsic aging is natural aging, an aging process caused by irresistible physiological factors with increasing age, belongs to gene-level aging, and is obviously characterized by the appearance of wrinkles and the laxity of the skin. Extrinsic aging is mainly aging caused by environmental factors, such as ultraviolet radiation, high temperature, cold, environmental pollution, nutritional deficiency, diseases, bad living habits, and the like, wherein ultraviolet radiation is the most important factor, and is therefore called photoaging, which is expressed by rough and dry skin exposed parts, wrinkle deepening and thickening, irregular pigmentation, vasodilatation, epidermal keratosis, dermal elasticity fibrosis, accumulation of degradation products, and the like, and can be theoretically prevented and slowed down. The theory of free radical aging states that excessive free radicals are considered to be an important cause of skin photoaging, and because the skin is exposed to external environments such as ultraviolet rays, active oxygen generated by the ultraviolet rays and active oxygen in other external environments generate various free radicals in the skin, so that the skin is damaged by oxidative stress, and finally, the skin is aged.
in general, dermal aging is manifested by a decrease in thickness, a decrease in synthesis and degradation of collagen and elastin, an increase in degradation of catabolic enzyme activity, a decrease in dermal thickness of 20-80% in the aging process, and a gradual degradation of tissues and functions, collagen is protein having the highest content in the human body, which imparts toughness, elasticity, water-proofing function, etc., and is an important raw material for maintaining the morphology and structure of the skin and tissue organs and repairing each damaged tissue, or is a main component of extracellular matrix.
BMP-1 (bone morphogenetic protein-1), which is the major protease in the assembly of extracellular matrix molecules, is involved in processing, primarily in the cleavage of peptide chains from proteins in the extracellular matrix (ECM), and can modify precursors of many biomolecules into biologically functional structural proteins and active enzymes, including many matrix molecules such as procollagen types I, III, V, and VII, Lipoxygenase (LOX), small leucine-rich proteoglycan (SLRP), etc. simply, BMP-1 functionalizes collagen, ensuring that collagen is modified in an ideal manner during biosynthesis, and that the correct protein conformation is formed.
Collagen is the gold standard in various indexes for resisting skin aging, but the efficacy and mechanism of most products on the cosmetic market are only about how to increase the collagen content (such as promoting generation, supplying and protecting). Recent studies have shown that the three-dimensional conformation and structure of collagen also have a significant effect on skin gloss. For the skin, collagen is a light propagation channel, and collagen fiber-mediated light scattering plays an important role in light propagation penetration inside the dermis. If the skin has high collagen content and normal and orderly arrangement structure, light can be directly projected into the skin, and the appearance of the skin has transparent luster; if the collagen is less, the structure is abnormal, and the arrangement is disordered, the diffuse reflection of the light path (the scattering effect of the collagen beam) is formed, so that the appearance is dark, yellow and matt.
Photoaging caused by UV exposure causes increased activity of skin elastase. Elastase is distributed in various tissues and cells, and the increase of elastase activity can cause the hydrolysis of skin elastic fibers, thereby accelerating the decomposition of collagen and elastin in dermis, leading the skin to lose elasticity, and causing skin aging phenomena such as wrinkles and the like.
The process of pigmentation is complex processes, mainly due to biochemical reactions within melanocytes, i.e. tyrosine, under the action of tyrosinase as a catalyst, produces dopaquinone, which in turn, under enzymatic or non-enzymatic oxidation, forms melanin, wherein the key is the synthesis of melanin, which is controlled by tyrosinase.
Therefore, the research on how to improve the anti-aging capability of the skin has important significance on delaying skin aging.
Extracts from natural plant sources are increasingly used in the field of skin care due to their long history of use, excellent multiple efficacy.
Mirabilis jalapa L is a plant of genus Mirabilis of family Mirabilis, also called as primula, Sophora subprostrata, Nostoc nocturna, Megalobus, and Eugenia caryophyllata. The native tropical America is cultivated in various places in China, namely south and north, and is an ornamental flower and sometimes a wild flower. The medicine is originally seen in Bencao gang mu Shi Yi (compendium of materia Medica), is cold in nature, sweet in taste, applicable to all kinds of medicines such as root, leaf, flower and seed, and can be used for treating symptoms such as heat stranguria, whitish and turbid urine, edema, leucorrhea with reddish discharge, joint swelling and pain, carbuncle, sore and pyogenic infections, and the seed powdery mildew can remove facial freckles and nevus. The research reports that the water extract of the mirabilis jalapa contains amino acids, saccharides, glycosides, organic acids, phenols, volatile oil and other substances, the alcohol extract contains phenols, cardiac glycosides, lactones, coumarins, flavones and other substances, and the petroleum ether extract mainly contains saponins and other substances.
Disclosure of Invention
The invention provides application of mirabilis jalapa extracts and a skin external preparation containing the same, aiming at solving the technical problem that active substances capable of improving the antioxidant and anti-aging capabilities of skin are lacked in the prior art.
The inventor discovers through a large amount of researches that: 1) mirabilis jalapa extract has free radical scavenging effect; 2) mirabilis jalapa extract has tyrosinase activity inhibiting effect; 3) the Mirabilis jalapa extract has effects of inhibiting elastase activity, improving anti-aging protein expression in human fibroblast, regulating synthesis of collagen in dermis, and delaying aging, and is suitable for cosmetics.
The invention mainly solves the technical problems by the following technical means:
the invention provides application of Mirabilis jalapa extracts in preparing or more of antioxidants, melanin synthesis inhibitors, elastic fiber hydrolysis inhibitors and anti-skin aging protein expression up-regulating agents.
In the present invention, the antioxidant may be a substance conventional in the art as , which helps to trap and neutralize free radicals, such as a free radical scavenger.
In the present invention, the melanin synthesis inhibitor may be a substance that inhibits melanin synthesis, such as a tyrosinase inhibitor, which is conventional in the art.
In the present invention, the elastic fiber hydrolysis inhibitor may be a substance that inhibits the hydrolysis of elastic fibers, which is conventional in the art, such as elastase inhibitor , wherein the hydrolysis of elastic fibers in the skin accelerates the decomposition of collagen and elastin in the dermis, thus causing the skin to lose elasticity and wrinkles.
In the present invention, the anti-skin aging protein may be a protein that is conventional in the art and is associated with skin aging, such as collagen and elastin.
In the present invention, the skin aging-resistant protein expression up-regulator may be any substance conventionally used in the art to affect the expression of skin aging-related proteins, such as or more of a BMP-1 (bone morphogenetic protein-1) expression up-regulator, a collagen I expression up-regulator and a collagen V expression up-regulator.
It is well known to those skilled in the art that BMP-1 can be processed to modify procollagen types I, III, V and VII to functionalize the collagen.
In the invention, the concentration of the mirabilis jalapa extract in the antioxidant is preferably 0.025-0.075 mg/mL, and more preferably 0.05 mg/mL.
In the present invention, the concentration of the mirabilis jalapa extract in the melanin synthesis inhibitor is preferably 0.25-0.75 mg/mL, and more preferably 0.5 mg/mL.
In the present invention, the concentration of the mirabilis jalapa extract in the elastic fiber hydrolysis inhibitor is preferably 0.5-1.5 mg/mL, and more preferably 1 mg/mL.
In the invention, the concentration of the mirabilis jalapa extract in the skin aging resistant protein expression up-regulator is preferably 0.05-1 mg/mL, such as 0.05mg/mL or 1 mg/mL.
When the anti-skin aging protein is BMP-1, the concentration of the Mirabilis jalapa extract in the up-regulator of the expression of the anti-skin aging protein is preferably 0.05 mg/mL.
When the anti-skin aging protein is collagen I or collagen V, the concentration of the mirabilis jalapa extract in the up-regulator of anti-skin aging protein expression is preferably 1 mg/mL.
In the present invention, the antioxidant, melanin synthesis inhibitor, elastic fiber hydrolysis inhibitor and anti-skin aging protein expression up-regulator are suitably used in cosmetics or skin care products.
In the invention, the Mirabilis jalapa extract can be prepared by a conventional method in the field, for example, the Mirabilis jalapa L is mixed with a solvent and extracted to obtain an extracting solution.
The extract part of Mirabilis jalapa can be parts of Mirabilis jalapa which can be used as medicine, such as kinds or more of Mirabilis jalapa root, Mirabilis leaf, Mirabilis jalapa flower and Mirabilis jalapa seed, preferably Mirabilis jalapa flower, and Mirabilis jalapa flower is obtained by harvesting Mirabilis jalapa L in bud stage.
When the extracting part of the mirabilis jalapa is mirabilis jalapa, the mirabilis jalapa extract is a mirabilis jalapa extract.
Wherein, the solvent can be an extraction solvent which is conventional in the field, and preferably ethanol. The concentration of the ethanol can be the conventional concentration in the field, and is preferably 60-80% ethanol water solution, and the percentage refers to volume percentage.
Wherein, the ratio of the quality of the mirabilis jalapa to the volume of the solvent can be a conventional ratio in the field, such as 1: (8-12) g/mL, preferably 1: 10 g/mL.
The extraction method can be conventional extraction method in the field, such as cold soaking extraction and heating extraction in sequence.
The time for the cold leaching extraction may be a time conventional in the art, for example 12 h.
The temperature for the heating extraction may be a temperature conventional in the art, for example, 50 to 70 ℃, preferably 60 ℃.
The time for the heating extraction can be a time conventional in the art, such as 1-3 h, preferably 2 h.
The number of times of the heating extraction may be a number of times conventional in the art, for example, 3 times. It is known to those skilled in the art that when the number of times of extraction is more than 1 by heating, the extracts should be combined.
Wherein the extract can be purified by conventional procedures in the art, for example, subjecting the extract to macroporous adsorbent resin chromatography.
Preferably, the extract is dried, redissolved with water and chromatographed on macroporous adsorbent resin.
The macroporous adsorption resin can be AB-8.
The eluent for chromatography may be an aqueous ethanol solution, the concentration of the aqueous ethanol solution is preferably 25 to 35%, more preferably 30%, and the percentage refers to volume percentage.
The volume of eluent for the chromatography may be 5 column volumes.
The chromatography may also be preceded by a depuration treatment, for example by elution with water, preferably in a volume of 1 column.
Preferably, the preparation method of the mirabilis jalapa extract comprises the following steps: mixing Mirabilis jalapa flower with ethanol water solution, sequentially performing cold soaking extraction and heating extraction to obtain extractive solution; the concentration of the ethanol water solution is 60-80%, and the percentage refers to volume percentage; the cold leaching extraction time is 12 hours; the temperature for heating and extracting is 50-70 ℃; the heating and extracting time is 1-3 h.
The invention also provides application of Mirabilis jalapa extracts as antioxidant active ingredients, whitening active ingredients and anti-aging active ingredients in cosmetics or skin care products.
Wherein the Mirabilis jalapa extract is as defined above.
The invention also provides skin external preparations, which contain 0.001-10% of mirabilis jalapa extract, wherein the percentage refers to the weight percentage (g/100mL) in the skin external preparation.
Wherein the Mirabilis jalapa extract is as defined above.
Wherein, the content of the mirabilis jalapa extract is preferably 0.0025-0.15%, such as 0.005%, 0.05% or 0.1%, and the percentage refers to the weight percentage in the skin external preparation.
When the mirabilis jalapa extract is used as an antioxidant in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.0025 to 0.0075%, more preferably 0.005%, and the percentage refers to the weight percentage in the skin external preparation.
When the mirabilis jalapa extract is used as a melanin synthesis inhibitor in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.025-0.075%, more preferably 0.05%, and the percentage refers to the weight percentage in the skin external preparation.
When the mirabilis jalapa extract is used as a skin elastic fiber hydrolysis inhibitor in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.05-0.15%, more preferably 0.1%, and the percentage refers to the weight percentage in the skin external preparation.
When the mirabilis jalapa extract is used as an up-regulator of expression of a skin aging-resistant protein in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.005 to 0.1%, for example, 0.005% or 0.1%, and the percentage refers to the weight percentage in the skin external preparation.
When the anti-skin aging protein is BMP-1, the content of the mirabilis jalapa extract is preferably 0.005%, and the percentage refers to the weight percentage in the external preparation for skin.
When the anti-skin aging protein is collagen I or collagen V, the content of the mirabilis jalapa extract is preferably 0.1%, by weight, in the skin external preparation.
In the present invention, the external preparation for skin refers to a general concept of all ingredients generally used for the external skin, and may be, for example, cosmetics or medicines. The cosmetic can be basic cosmetics, face makeup cosmetics, body makeup cosmetics, head care products and the like, and the dosage form of the cosmetic is not particularly limited and can be reasonably selected according to different purposes.
In the present invention, the external preparation for skin may further comprise other active ingredients, preferably or more of moisturizing active ingredients, antioxidant active ingredients, oil-controlling active ingredients, whitening active ingredients and anti-aging active ingredients, in an amount conventional in the art.
The external preparation for skin may further contain a vehicle or a base excipient conventionally used in the art according to various formulations and purposes, as long as the excipient is an acceptable excipient in the cosmetic or pharmaceutical field, in accordance with the general knowledge in the art. The excipient content is the content conventional in the art.
Wherein the excipient may be in the form of a water phase, an oil phase, a gel, a wax-in-water emulsion, an oil-in-water emulsion or a water-in-oil emulsion.
The skin external preparation of the present invention may further comprise step any other components conventionally used in the cosmetic field, for example, or more of preservatives, fragrances, hydrophilic active agents and lipophilic active agents.
In the present invention, the external preparation for skin may further comprise a particulate phase, and the particulate phase may be or more selected from pigments, pearling agents and fillers.
In the present invention, the form of the skin external preparation may be any suitable product form including, but not limited to, or more of aerosol type spray, cream, emulsion, solid, liquid, dispersion, foam, gel, lotion, mousse, ointment, powder, patch, pomade, hand pump type spray, stick, pack and towelette.
In a preferred embodiment of the present invention, the external skin preparation is a cosmetic water, and the external skin preparation comprises, by weight, 5% of glycerin, 3 to 6% of a polyhydric alcohol A, 1% of betaine, 0.5% of panthenol, 0.05 to 0.1% of dipotassium glycyrrhizinate, 0.15 to 0.2% of polyethylene glycol-320.5%, 0.15 to 0.2% of a surfactant A, 0.4 to 0.45% of a preservative A, 0 to 0.15% of xanthan gum, 0.03% of a fragrance, and 0.001 to 10% of the mirabilis jalapa extract, and the balance to 100% of deionized water, wherein the polyhydric alcohol A is or more selected from butylene glycol, 1, 2-pentanediol, and dipropylene glycol, the surfactant A is or more selected from polysorbate-20 and PEG-40 hydrogenated castor oil, and the preservative A is methylparaben and/or phenoxyethanol.
Wherein, the mirabilis jalapa extract is preferably a mirabilis jalapa extract. The content of the mirabilis jalapa flower extract is preferably 0.0025 to 0.15%, more preferably 0.005 to 0.1%, for example 0.005%, by weight in the skin external preparation.
In a second preferred embodiment of the present invention, the external skin preparation is a serum, and the external skin preparation comprises, by weight, 5% of glycerin, 3 to 6% of a polyol B, 1% of panthenol, 0.1% of dipotassium glycyrrhizinate, 0.1% of allantoin, 0.05 to 3.6% of a surfactant B, 0.25 to 0.45% of a preservative B, 0.2 to 0.4% of xanthan gum, 0.05% of an acrylic resin Pemulen TR-20.3% and 0.05% of a fragrance, 0.001 to 10% of a Mirabilis jalapa extract, and the balance of deionized water to 100%, wherein the polyol B is butylene glycol and/or 1, 3-propylene glycol, the surfactant B is polyglycerol-2 oleate and/or polyglycerol-10 oleate, and the preservative B is or more of propylhydroxybenzoate, methylparaben and phenoxyethanol.
According to the common knowledge in the field, the acrylic resin Pemulen TR-2 is acrylic acid (ester)/C10-30Alkanol acrylate cross-linked polymers, produced by nuch chemical company, usa.
The xanthan gum is a saccharide, produced by fermentation of xanthomonas sp, according to common knowledge in the art, as extracellular microbial polysaccharides.
Wherein, the mirabilis jalapa extract is preferably a mirabilis jalapa extract. The content of the mirabilis jalapa flower extract is preferably 0.0025 to 0.15%, more preferably 0.005 to 0.1%, for example 0.1%, and the percentage refers to the weight percentage in the skin external preparation.
In a third preferred embodiment of the present invention, the external skin preparation is an emulsion, and the external skin preparation comprises the following components in percentage by weight: 5% of glycerol, 5% of 1, 3-propylene glycol, 1% of panthenol, 0.5% of tocopherol acetate, 2% of polydimethylsiloxane, 1652% of emulsifier A, 2-3% of oil C, 3% of isopropyl isostearate, 0.5% of cetostearyl alcohol, 0.3% of preservative C, 0.5% of xanthan gum and 0.1% of EDTA disodium; the skin external agent also comprises triethanolamine, and the content of the triethanolamine is based on adjusting the pH value of the skin external agent to 5.5-7; and 0.001% -10% of the mirabilis jalapa extract; the rest is supplemented to 100 percent by deionized water; wherein the oil C is mineral oil and/or isohexadecane; the preservative C is methyl hydroxybenzoate and/or propyl hydroxybenzoate.
The emulsifier A165 is a commercially available product containing glyceryl stearate and PEG-100 stearate, as is common in the art. The hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer is a thickening agent which is produced by Sabic corporation and has the mark of Sepinov EMT 10.
Wherein, the mirabilis jalapa extract is preferably a mirabilis jalapa extract. The content of the mirabilis jalapa flower extract is preferably 0.0025 to 0.15%, more preferably 0.005 to 0.1%, for example 0.005%, by weight in the skin external preparation.
In a fourth preferred embodiment of the present invention, the external preparation for skin is a cream, and the external preparation for skin comprises the following components in percentage by weight: 5% of glycerol, 5% of 1, 3-propylene glycol, 1% of panthenol, 0.5% of tocopherol acetate, 2% of polydimethylsiloxane, 1652% of emulsifier A, 2-8% of oil D, 3% of isopropyl isostearate, 2-3% of cetostearyl alcohol, 0.3% of preservative D, 2% of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer and 0.1% of disodium EDTA; the skin external agent also comprises triethanolamine, and the content of the triethanolamine is based on adjusting the pH value of the skin external agent to 5.5-7; 0.001% -10% of mirabilis jalapa extract; the rest is supplemented to 100 percent by deionized water; wherein the oil D is mineral oil and/or isohexadecane; the preservative D is methyl hydroxybenzoate and/or propyl hydroxybenzoate.
Wherein, the mirabilis jalapa extract is preferably a mirabilis jalapa extract. The content of the mirabilis jalapa flower extract is preferably 0.0025 to 0.15%, more preferably 0.005 to 0.1%, for example 0.1%, and the percentage refers to the weight percentage in the skin external preparation.
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
The reagents and starting materials used in the present invention are commercially available.
The positive progress effects of the invention are as follows:
(1) the application discovers that the mirabilis jalapa extract, particularly the mirabilis jalapa extract, has the effects of eliminating free radical activity, inhibiting tyrosinase activity, inhibiting elastase activity and improving anti-aging protein expression in human fibroblasts, can regulate and control synthesis of collagen in dermis, has the effect of delaying aging, and particularly has the effect of improving anti-aging protein expression in human fibroblasts. Compared with a blank control, the generation rates of collagen I, collagen V and BMP1 in the fibroblasts are 133%, 125% and 150% respectively, and the method has important significance for developing skin care products or cosmetics related to anti-aging in the future.
(2) The application discovers for the first time that the mirabilis jalapa extract, particularly the mirabilis jalapa extract, can promote the generation of bone morphogenetic protein-1 (BMP-1), and has important significance for the research on the action mechanism of the mirabilis jalapa extract and the active ingredients acting on the BMP-1 target.
Detailed Description
The invention is further illustrated by the following examples , but is not intended to be limited thereby within the scope of the examples.
Example 1 preparation of Mirabilis jalapa extract
Weighing 100g of dried mirabilis jalapa, crushing, adding 100mL of 70% ethanol, and cold soaking for 12 hours until the solvent fully soaks the material. Heating to 60 deg.C, extracting for 2 hr, filtering, collecting extractive solution, and extracting for 3 times. Mixing the three extractive solutions, and vacuum drying under reduced pressure to obtain 15g crude extract. Weighing 1g of crude extract, dissolving with water, passing through macroporous adsorbent resin AB-8, eluting with 1 column volume of water, and discarding water eluate. Then eluting with 30% ethanol with 5 times of column volume, concentrating the eluate, and evaporating to obtain Mirabilis jalapa flower extract dry powder 0.5 g.
Example 2
The toning lotion is prepared from the following components in percentage by weight:
5% of glycerin, 3% of butanediol, 1% of betaine, 0.5% of panthenol, 0.1% of dipotassium glycyrrhizinate, 2.0% of allantoin, 0.15-0.2% of polyethylene glycol-320.5%, 0.15-0.2% of hydroxyethyl cellulose, 450.4% of IS-xanthan gum, 0.03% of perfume and 0.005% of mirabilis jalapa extract; the remainder was made up to 100% with deionized water.
The skin external preparation in this example was prepared according to a conventional method for preparing a cosmetic lotion in the art.
Example 3
The essence is prepared from the following components in percentage by weight:
5% of glycerin, 3% of butanediol, 1% of panthenol, 0.1% of dipotassium glycyrrhizinate, 0.1% of allantoin, 4% of nicotinamide, 0.05% of hydroxyethyl cellulose, 0.15% of methylparaben, 0.3% of phenoxyethanol, 0.2% of xanthan gum, 20.3% of acrylic resin Pemulen TR, 0.05% of perfume and 0.1% of mirabilis jalapa extract; the remainder was made up to 100% with deionized water.
The skin external preparation in this example was prepared according to a conventional preparation method of essence in the art.
Example 4
The emulsion is prepared from the following components in percentage by weight:
5% of glycerol, 5% of 1, 3-propylene glycol, 1% of panthenol, 0.5% of tocopherol acetate, 0.1% of dipotassium glycyrrhizinate, 2% of polydimethylsiloxane, 1652% of an emulsifier A, 3% of isopropyl isostearate, 3% of mineral oil, 0.5% of cetostearyl alcohol, 0.2% of methyl hydroxybenzoate, 0.1% of propyl hydroxybenzoate, 0.5% of xanthan gum and 0.1% of disodium EDTA, and regulating the pH value of the emulsion to 5.5-7 by triethanolamine; mirabilis jalapa flower extract 0.005%; the remainder was made up to 100% with deionized water.
The skin external preparation in this example was prepared according to the conventional emulsion preparation method in the art.
Example 5
The cream is prepared from the following components in percentage by weight:
5% of glycerol, 5% of 1, 3-propylene glycol, 1% of panthenol, 0.5% of tocopherol acetate, 0.1% of dipotassium glycyrrhizinate, 2% of polydimethylsiloxane, 1652% of an emulsifier A, 3% of isopropyl isostearate, 2% of mineral oil, 2% of cetostearyl alcohol, 0.2% of methyl hydroxybenzoate, 0.1% of propyl hydroxybenzoate, 2% of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer and 0.1% of disodium EDTA; regulating the pH value of the emulsion to 5.5-7 by triethanolamine; mirabilis jalapa flower extract 0.1%; the remainder was made up to 100% with deionized water.
The skin external preparation in this example was prepared according to a conventional method for preparing a cream in the art.
Effect example 1 activity assay of mirabilis jalapa extract
(1) Detection of activity of scavenging free radicals by DPPH method
1, 1-Diphenyl-2-trinitrophenylhydrazine (1, 1-Diphenyl-2-pentacylhydrazyl, DPPH) is stable nitrogen center organic free radicals, the ethanol solution of the stable nitrogen center organic free radicals is dark purple and has strong absorption about 517nm, when a free radical scavenger exists, the DPPH absorption is weakened and gradually disappears due to single electron pairing with the DPPH ethanol solution, the fading degree of the DPPH ethanol solution and the number of electrons received by the DPPH ethanol solution are in a quantitative relation, so that a spectrophotometer can be used for carrying out rapid quantitative analysis, detecting the free radical scavenging condition, and evaluating the free radical scavenging capacity, the oxidation resistance and the anti-aging capacity of an antioxidant.
Preparing a DPPH ethanol solution: 6mg of DPPH was weighed out accurately, dissolved in 95% ethanol and placed in a 50mL volumetric flask, and dissolved with the aid of ultrasound. When in use, the mixture is diluted by four times by 95% ethanol to obtain a DPPH ethanol solution with the concentration of 3mg/100mL (76 mu M), and the DPPH ethanol solution is stored in a dark place (0-4 ℃).
Pretreating a sample to be detected: the mirabilis jalapa flower extract prepared in example 1 and an ascorbic acid standard are dissolved in deionized water and diluted to a suitable concentration with the deionized water for later use.
The experimental steps are as follows: adding 2mL of sample solution to be detected into a test tube, then adding 2mL of ethanol solution of LDPPH, and uniformly mixing. Reacting in dark place at room temperature for 30min in the dark place in a dark place, and measuring absorbance Abs at 525 nm; except for the sample group, a sample solvent control group, a blank control group and a solvent control group (solvent of DPPH) are respectively arranged, and the positive control group is an ascorbic acid group.
Clearance I (%) ═ 1- (T-T)0)/(C-C0)]×100％
In the formula:
T0: adding the absorbance of the sample solution to be detected with equal volume of 95% ethanol (sample solvent control group);
t: adding the absorbance of the sample solution to be detected and an isopyknic DPPH solution (sample group);
C0: absorbance of distilled water plus equal volume of 95% ethanol (solvent control);
c: absorbance of distilled water plus an equal volume of DPPH solution (blank control).
TABLE 1 measurement results of radical scavenging action
As shown in Table 1, Mirabilis jalapa extract has DPPH radical scavenging effect. Compared with a blank control group, the radical clearance rate of the mirabilis jalapa extract group is 76%.
(2) Detection of tyrosinase Activity inhibition Rate
The experimental steps are as follows: adding phosphate buffer solution (pH6.8, 30mM)70 μ L, 1.66mM tyrosinase solution 80 μ L, and sample solution 80 μ L into 96-well plate, mixing, incubating at 37 deg.C for more than 5 min, adding tyrosinase solution (125U/ml) (from Sigma, product number T3824)10 μ L, incubating at 37 deg.C for 10min, and measuring absorbance A at 475nm wavelength with enzyme-labeling instrument475The sample solution includes a solution prepared from the mirabilis jalapa extract prepared in example 1 and an arbutin standard solution (both diluted to a suitable concentration by deionization), wherein the arbutin standard solution is used as a positive control group. The deionized water is used for replacing the sample water solution, the absorbance is also measured as a reference solution, and the tyrosinase activity inhibition rate calculation formula is as follows: inhibition ratio (%) ═ a0-(A475-B))/A0X 100% where A0Is the absorbance of a reference solution, A475Is the absorbance of the sample solution, and B is the absorbance of the sample blank solution.
TABLE 2 determination of tyrosinase inhibitory Activity
As can be seen from Table 2, Mirabilis jalapa flower extract has tyrosinase inhibitory activity. Compared with a blank control group, the tyrosinase inhibition rate of the mirabilis jalapa extract group is 44%.
(3) Detection of Elastase Activity inhibition
The experimental steps are as follows: mu.L of the sample solution and 130. mu.L of 0.1M Tris-HCl buffer solution (pH8.0) containing 1.015mM of the reaction substrate Succ-Ala-Ala-Ala-p-nitroanilide were added to a 96-well plate, incubated at 25 ℃ for 5 minutes, 15. mu.L of an elastase solution (0.5U/mL) was added, incubation at 25 ℃ was continued for 30 minutes, and then the absorbance A at 410nm was measured with a microplate reader410The sample solution includes a prepared solution of the mirabilis jalapa extract prepared in example 1 and an Elastatinal solution (both diluted to an appropriate concentration by deionization), wherein the Elastatinal solution (elastase inhibitor, available from sigma, cat # E0881) is used as a positive control group. The absorbance was also measured as a reference solution by replacing the sample aqueous solution with deionized water.
The calculation formula of the elastase activity inhibition rate is as follows: inhibition ratio (%) ═ a0-(A410-B))/A0X 100% where A0Is the absorbance of a reference solution, A410Is the absorbance of the sample solution, and B is the absorbance of the sample blank solution.
TABLE 3 measurement results of elastase activity inhibition
As shown in Table 3, Mirabilis jalapa flower extract had an elastase inhibitory effect. The inhibition rate of elastase in the mirabilis jalapa extract group is 26% compared with that of the blank control group.
(4) Fibroblast collagen I production rate assay
Human fibroblasts were seeded in a 96-well plate (5000 cells/well), the culture medium was removed after 72 hours of culture, a solution prepared from the mirabilis jalapa extract prepared in example 1 and an ascorbic acid standard solution (both diluted to appropriate concentrations with deionized water) were added, and further culture was carried out for 48 hours, after the supernatant was removed, the content of COL I (collagen I) in the cells was measured by the immunofluorescence assay, and at the same time, the DNA content of the cell layer (picogreen kit, purchased from thermo fisher) was measured to standardize the content data of COL I. The test results will indicate that the blank control group had 100% production of type I stock collagen compared to the untested control group. The formation rate is more than 100%, which is the promotion effect.
TABLE 4 COL I content measurement results
As can be seen from table 4, the mirabilis jalapa extract had the effect of promoting collagen I secretion, and the COL I production rate in human fibroblasts of the mirabilis jalapa extract-treated group was 133%, compared to the blank control group.
(5) Fibroblast collagen V production rate assay
Human fibroblasts were seeded in a 96-well plate (5000 cells/well), the culture medium was removed after 72 hours of culture, a solution prepared from the mirabilis jalapa extract prepared in example 1 and an ascorbic acid standard solution (both diluted to appropriate concentrations with deionized water) were added, and further culture was carried out for 48 hours, after the supernatant was removed, the content of COL V (collagen V) in the cells was measured by the cytoimmunofluorescence method, and at the same time, the DNA content of the cell layer (picogreen kit, purchased from thermo fisher) was measured to standardize the content data of COL V. The test result shows that the generation rate of V-type stored collagen of the blank control group is 100 percent compared with the control group which is not tested. The formation rate is more than 100%, which is the promotion effect.
TABLE 5 measurement results of COL V content
As can be seen from table 5, the mirabilis jalapa extract has the effect of promoting collagen V secretion, and compared with the blank control group, the percentage of COL V production in human fibroblasts of the mirabilis jalapa extract-treated group can reach 125%.
(6) Fibroblast BMP1 production Rate determination
Planting human fibroblasts into a 96-well plate (5000 cells/well), removing a culture medium after culturing for 72 hours, adding a solution prepared from the mirabilis jalapa extract prepared in example 1 (diluted to a proper concentration by deionization), culturing for 24 hours, collecting cell culture supernatant, and measuring the content of BMP1 in the cell culture supernatant by adopting a BMP1ELISA kit; the BMP1 content data were normalized by simultaneously determining the DNA content of the cell layer (picogreen kit, from thermo fisher). The test results showed 100% production of BMP1 in the blank control compared to the control that was not tested. The formation rate is more than 100%, which is the promotion effect.
TABLE 6 measurement of BMP1 content
As shown in Table 6, Mirabilis jalapa extract has an effect of promoting BMP 1. Compared with a blank control group, the generation rate of BMP1 in the human fibroblasts of the Mirabilis jalapa extract treatment group can reach 150%.
Claims (10)
- Application of Mirabilis jalapa extracts in preparing or more of antioxidant, melanin synthesis inhibitor, elastic fiber hydrolysis inhibitor and skin aging protein expression up-regulating agent is provided.
- 2. The use of claim 1, wherein the antioxidant is a free radical scavenger;and/or the melanin synthesis inhibitor is a tyrosinase inhibitor;and/or the inhibitor of elastane hydrolysis is an elastase inhibitor;and/or, the skin aging resistant protein expression up-regulator is or more of BMP-1 expression up-regulator, collagen I expression up-regulator and collagen V expression up-regulator.
- 3. The use according to claim 1 or 2,when the mirabilis jalapa extract is used for preparing an antioxidant, the concentration of the mirabilis jalapa extract in the antioxidant is 0.025-0.075 mg/mL, and preferably 0.05 mg/mL;when the mirabilis jalapa extract is used for preparing a melanin synthesis inhibitor, the concentration of the mirabilis jalapa extract in the melanin synthesis inhibitor is 0.25-0.75 mg/mL, preferably 0.5 mg/mL;when the mirabilis jalapa extract is used for preparing the elastic fiber hydrolysis inhibitor, the concentration of the mirabilis jalapa extract in the elastic fiber hydrolysis inhibitor is 0.5-1.5 mg/mL, preferably 1 mg/mL;when the mirabilis jalapa extract is used for preparing the skin aging resistant protein expression up-regulator, the concentration of the mirabilis jalapa extract in the skin aging resistant protein expression up-regulator is 0.05-1 mg/mL; preferably: when the anti-skin aging protein is BMP-1, the concentration of the Mirabilis jalapa extract in the up-regulator of the anti-skin aging protein expression is 0.05 mg/mL; when the anti-skin aging protein is collagen I or collagen V, the concentration of the mirabilis jalapa extract in the anti-skin aging protein expression up-regulator is 1 mg/mL.
- 4. The use of claim 1, wherein the Mirabilis jalapa extract is prepared by a method comprising the steps of: mixing Mirabilis jalapa L.with solvent, and extracting to obtain extractive solution.
- 5. The use of claim 4, wherein the Mirabilis jalapa extraction site is Mirabilis jalapa flower;and/or the solvent is ethanol water solution, the concentration of the ethanol water solution is preferably 60-80%, and the percentage refers to volume percentage;and/or the ratio of the mass of the mirabilis jalapa to the volume of the solvent is 1: (8-12) g/mL, preferably 1: 10 g/mL;and/or the extraction method comprises the steps of cold soaking extraction and heating extraction in sequence; the time of the cold leaching extraction is preferably 12 hours; the temperature for heating and extracting is preferably 50-70 ℃, more preferably 60 ℃; the heating extraction time is preferably 1-3 h, more preferably 2 h; the number of times of the heating extraction is preferably 3;and/or, the extracting solution is also subjected to purification treatment; preferably, the extract is chromatographed by macroporous adsorption resin; preferably, the extracting solution is dried, redissolved by water and then subjected to macroporous adsorption resin chromatography; the macroporous adsorption resin is preferably AB-8; the eluent for chromatography is preferably ethanol water solution, the concentration of the ethanol water solution is preferably 25-35%, more preferably 30%, and the percentage refers to volume percentage; the volume of eluent for the chromatography is preferably 5 column volumes; the chromatography is preferably further subjected to impurity removal treatment, more preferably to impurity removal by elution with water, and the elution volume of the water is preferably 1 column volume.
- 6, kinds of external preparations for skin, characterized in that, it contains 0.001-10% Mirabilis jalapa extract, the percentage is the weight percentage in the external preparations for skin;preferably, the preparation method of the mirabilis jalapa extract comprises the following steps: mixing Mirabilis jalapa L.with solvent, and extracting to obtain extractive solution.
- 7. The external preparation for skin as claimed in claim 6, wherein the extract site of Mirabilis jalapa is Mirabilis jalapa flower;and/or the solvent is ethanol water solution, the concentration of the ethanol water solution is preferably 60-80%, and the percentage refers to volume percentage;and/or the ratio of the mass of the mirabilis jalapa to the volume of the solvent is 1: (8-12) g/mL, preferably 1: 10 g/mL;and/or the extraction method comprises the steps of cold soaking extraction and heating extraction in sequence; the time of the cold leaching extraction is preferably 12 hours; the temperature for heating and extracting is preferably 50-70 ℃, more preferably 60 ℃; the heating extraction time is preferably 1-3 h, more preferably 2 h; the number of times of the heating extraction is preferably 3;and/or, the extracting solution is also subjected to purification treatment; preferably, the extract is chromatographed by macroporous adsorption resin; preferably, the extracting solution is dried, redissolved by water and then subjected to macroporous adsorption resin chromatography; the macroporous adsorption resin is preferably AB-8; the eluent for chromatography is preferably ethanol water solution, the concentration of the ethanol water solution is preferably 25-35%, more preferably 30%, and the percentage refers to volume percentage; the volume of eluent for the chromatography is preferably 5 column volumes; the chromatography is preferably further subjected to impurity removal treatment, more preferably to impurity removal by elution with water, and the elution volume of the water is preferably 1 column volume.
- 8. The external preparation for skin as claimed in claim 6 or 7,the content of the mirabilis jalapa extract is 0.0025-0.15%, preferably 0.005%, 0.05% or 0.1%, and the percentage refers to the weight percentage of the mirabilis jalapa extract in the skin external preparation;when the mirabilis jalapa linnaeus extract is used as an antioxidant in the skin external preparation, the content of the mirabilis jalapa linnaeus extract is preferably 0.0025 to 0.0075 percent, more preferably 0.005 percent, and the percent refers to the weight percent in the skin external preparation;when the mirabilis jalapa extract is used as a melanin synthesis inhibitor in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.025-0.075%, more preferably 0.05%, and the percentage refers to the weight percentage in the skin external preparation;when the mirabilis jalapa extract is used as a skin elastic fiber hydrolysis inhibitor in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.05-0.15%, more preferably 0.1%, and the percentage refers to the weight percentage in the skin external preparation;when the mirabilis jalapa extract is used as an up-regulator of the expression of anti-skin aging proteins in the skin external preparation, the content of the mirabilis jalapa extract is preferably 0.005-0.1%, more preferably 0.005% or 0.1%, and the percentage refers to the weight percentage in the skin external preparation; more preferably: when the anti-skin aging protein is BMP-1, the content of the Mirabilis jalapa extract is 0.005%, and the percentage refers to the weight percentage in the skin external preparation; when the skin aging resistant protein is collagen I or collagen V, the content of the Mirabilis jalapa extract is 0.1%, and the percentage refers to the weight percentage in the skin external preparation.
- 9. The external preparation for skin as claimed in claim 6 or 7, further comprising kinds of other active ingredients, preferably moisture-retaining active ingredient, antioxidant active ingredient, oil-controlling active ingredient, whitening active ingredient and anti-aging active ingredient;and/or, the skin external agent further comprises a medium or a matrix excipient, wherein the excipient is preferably in the form of a water phase, an oil phase, a gel, a wax-in-water type emulsion, an oil-in-water type emulsion or a water-in-oil type emulsion;and/or, the skin external agent further comprises or more of a preservative, a fragrance, a hydrophilic active agent and a lipophilic active agent;and/or, the skin external agent further comprises a particulate phase, wherein the particulate phase is or more of pigments, pearling agents and fillers.
- 10. The external preparation for skin as claimed in claim 9,when the external skin preparation is a toning lotion, the external skin preparation comprises, by weight, 5% of glycerin, 3-6% of a polyol A, 1% of betaine, 0.5% of panthenol, 0.05-0.1% of dipotassium glycyrrhizinate, 0.15-0.2% of polyethylene glycol-320.5%, 0.15-0.2% of a surfactant A, 0.4-0.45% of a preservative A, 0-0.15% of xanthan gum, 0.03% of a perfume and 0.001-10% of a mirabilis jalapa extract, and the balance being 100% by weight with deionized water, wherein the polyol A is or more of butylene glycol, 1, 2-pentanediol and dipropylene glycol, the surfactant A is polysorbate-20 and/or PEG-40 hydrogenated castor oil, the preservative A is methyl hydroxybenzoate and/or phenoxyethanol, the mirabilis jalapa preferably is a mirabilis jalapa jasmine extract, and the content of the mirabilis jalapa preferably is 0.0025-0.15%, more preferably 0.005-0.1%, and the percentage by weight of the external skin preparation is in percentage by weight;when the external skin preparation is essence, the external skin preparation comprises, by weight, 5% of glycerin, 3-6% of a polyol B, 1% of panthenol, 0.1% of dipotassium glycyrrhizinate, 0.1% of allantoin, 0.05-3.6% of a surfactant B, 0.25-0.45% of a preservative B, 0.2-0.4% of xanthan gum, 0.20.3% of an acrylic resin Pemulen TR-20.3% of a fragrance, and 0.05-10% of a Mirabilis jalapa extract, and the balance being supplemented to 100% with deionized water, wherein the polyol B is butylene glycol and/or 1, 3-propylene glycol, the surfactant B is polyglycerol-2 oleate and/or polyglycerol-10 oleate, the preservative B is or more of propylparaben, methylparaben and phenoxyethanol, the Mirabilis jalapa extract preferably contains 0.0025-0.15%, more preferably 0.005-0.005% of the weight percentage of the external skin preparation;when the skin external agent is emulsion, the skin external agent comprises the following components in percentage by weight: 5% of glycerol, 5% of 1, 3-propylene glycol, 1% of panthenol, 0.5% of tocopherol acetate, 2% of polydimethylsiloxane, 1652% of emulsifier A, 2-3% of oil C, 3% of isopropyl isostearate, 0.5% of cetostearyl alcohol, 0.3% of preservative C, 0.5% of xanthan gum and 0.1% of EDTA disodium; the skin external agent also comprises triethanolamine, and the content of the triethanolamine is based on adjusting the pH value of the skin external agent to 5.5-7; and 0.001% -10% of the mirabilis jalapa extract; the rest is supplemented to 100 percent by deionized water; wherein the oil C is mineral oil and/or isohexadecane; the preservative C is methyl hydroxybenzoate and/or propyl hydroxybenzoate; the mirabilis jalapa extract is preferably a mirabilis jalapa extract, and the content of the mirabilis jalapa extract is preferably 0.0025-0.15%, more preferably 0.005-0.1%, and the percentage refers to the weight percentage of the mirabilis jalapa extract in the skin external preparation;when the external skin preparation is a cream, the external skin preparation comprises the following components in percentage by weight: 5% of glycerol, 5% of 1, 3-propylene glycol, 1% of panthenol, 0.5% of tocopherol acetate, 2% of polydimethylsiloxane, 1652% of emulsifier A, 2-8% of oil D, 3% of isopropyl isostearate, 2-3% of cetostearyl alcohol, 0.3% of preservative D, 2% of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer and 0.1% of disodium EDTA; the skin external agent also comprises triethanolamine, and the content of the triethanolamine is based on adjusting the pH value of the skin external agent to 5.5-7; 0.001% -10% of mirabilis jalapa extract; the rest is supplemented to 100 percent by deionized water; wherein the oil D is mineral oil and/or isohexadecane; the preservative D is methyl hydroxybenzoate and/or propyl hydroxybenzoate; the mirabilis jalapa extract is preferably a mirabilis jalapa extract, and the content of the mirabilis jalapa extract is preferably 0.0025-0.15%, more preferably 0.005-0.1%, and the percentage refers to the weight percentage of the skin external preparation.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810804107.XA CN110731919B (en) | 2018-07-20 | 2018-07-20 | Application of mirabilis jalapa extract and skin external preparation containing mirabilis jalapa extract |
| PCT/CN2019/096711 WO2020015725A1 (en) | 2018-07-20 | 2019-07-19 | Use of mirabilis jalapa l. extracts and skin external preparations comprising the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810804107.XA CN110731919B (en) | 2018-07-20 | 2018-07-20 | Application of mirabilis jalapa extract and skin external preparation containing mirabilis jalapa extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110731919A true CN110731919A (en) | 2020-01-31 |
| CN110731919B CN110731919B (en) | 2022-07-12 |
Family
ID=69164157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810804107.XA Active CN110731919B (en) | 2018-07-20 | 2018-07-20 | Application of mirabilis jalapa extract and skin external preparation containing mirabilis jalapa extract |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN110731919B (en) |
| WO (1) | WO2020015725A1 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111973509A (en) * | 2020-08-19 | 2020-11-24 | 澳宝化妆品（惠州）有限公司 | Skin care composition containing plant extract for soothing allergy |
| CN114392215A (en) * | 2021-12-29 | 2022-04-26 | 珠海金肽生物科技有限公司 | Composition containing plant callus extract and application thereof |
| CN115177566A (en) * | 2022-07-28 | 2022-10-14 | 杭州舒彩网络科技有限公司 | Synergistic hydroxy pinacolone retinoic acid ester anti-aging essence and preparation method thereof |
| CN115400058A (en) * | 2022-09-20 | 2022-11-29 | 杭州舒彩网络科技有限公司 | Composition for improving skin cell activity and application of composition in skin external product |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113940905B (en) * | 2021-06-25 | 2022-06-17 | 太和康美(北京)中医研究院有限公司 | Face-beautifying cream and preparation method thereof |
| CN113995084B (en) * | 2021-11-18 | 2023-08-11 | 桂林理工大学 | Extraction method and application of jasmine flower extract |
| CN115444802B (en) * | 2022-09-05 | 2023-06-23 | 福州萍晴日用化妆品有限公司 | Jasmine moisturizing essence emulsion composition and preparation method thereof |
| CN115844746A (en) * | 2022-09-28 | 2023-03-28 | 山东宽源生物科技有限公司 | Anti-inflammatory and repairing bodhi cream and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103816085A (en) * | 2014-03-03 | 2014-05-28 | 西北大学 | Mirabilis jalapa seed endosperm extract and application thereof in cosmetics |
| WO2015140679A1 (en) * | 2014-03-17 | 2015-09-24 | Sederma | Cosmetic use of an extract of mirabilis jalapa |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2818141B1 (en) * | 2000-12-14 | 2003-09-12 | Sederma Sa | COSMETIC OR DERMOPHARMACEUTICAL COMPOSITIONS CONTAINING VEGETABLE EXTRACTS FROM BELLE DE NUIT (MIRABILIS JALAPA) AGAINST INFLAMMATION AND SKIN DROUGHT OF ANY ORIGIN |
| FR2837702B1 (en) * | 2002-03-26 | 2005-01-14 | Clarins Lab | COSMETIC COMPOSITION FOR SKIN CARE PARTICULARLY AS NIGHT CARE |
| CN105816416A (en) * | 2016-04-20 | 2016-08-03 | 广州雷诺生物科技有限公司 | Comforting, pleasing and moisturizing cream and production method thereof |
| CN107137269A (en) * | 2017-05-13 | 2017-09-08 | 王忠良 | A kind of whitening patch-lightening cosmetic and preparation method thereof |
-
2018
- 2018-07-20 CN CN201810804107.XA patent/CN110731919B/en active Active
-
2019
- 2019-07-19 WO PCT/CN2019/096711 patent/WO2020015725A1/en active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103816085A (en) * | 2014-03-03 | 2014-05-28 | 西北大学 | Mirabilis jalapa seed endosperm extract and application thereof in cosmetics |
| WO2015140679A1 (en) * | 2014-03-17 | 2015-09-24 | Sederma | Cosmetic use of an extract of mirabilis jalapa |
Non-Patent Citations (3)
| Title |
|---|
| JYOTCHNA GOGOI, ET AL.: "Isolation and characterization of bioactive components from Mirabilis jalapa L.radix", 《JOURNAL OF TRADITIONAL AND COMPLEMENTARY MEDICINE》, vol. 6, no. 1, 31 January 2016 (2016-01-31), pages 41 - 47 * |
| 罗艺萍 等: "紫茉莉根中油脂化合物的气相色谱-质谱分析", 《云南化工》 * |
| 罗艺萍 等: "紫茉莉根中油脂化合物的气相色谱-质谱分析", 《云南化工》, vol. 40, no. 1, 28 February 2013 (2013-02-28), pages 39 - 42 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111973509A (en) * | 2020-08-19 | 2020-11-24 | 澳宝化妆品（惠州）有限公司 | Skin care composition containing plant extract for soothing allergy |
| CN114392215A (en) * | 2021-12-29 | 2022-04-26 | 珠海金肽生物科技有限公司 | Composition containing plant callus extract and application thereof |
| CN115177566A (en) * | 2022-07-28 | 2022-10-14 | 杭州舒彩网络科技有限公司 | Synergistic hydroxy pinacolone retinoic acid ester anti-aging essence and preparation method thereof |
| CN115400058A (en) * | 2022-09-20 | 2022-11-29 | 杭州舒彩网络科技有限公司 | Composition for improving skin cell activity and application of composition in skin external product |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110731919B (en) | 2022-07-12 |
| WO2020015725A1 (en) | 2020-01-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110731919B (en) | Application of mirabilis jalapa extract and skin external preparation containing mirabilis jalapa extract | |
| CN107567329B (en) | Fermented extract of eupenicillium crustaceum and cosmetic use thereof | |
| KR100825450B1 (en) | Skin anti-wrinkle cosmetics composition containing Forsythiae Fruit extract | |
| TW201206494A (en) | Use of Tiliacora triandra in cosmetics and compositions thereof | |
| SE542639C2 (en) | PLANT EXTRACTS | |
| US8722627B2 (en) | Proteasome-activating lightening peptidic hydrolyzates and compositions containing them | |
| KR102349786B1 (en) | Cosmetic composition for anti-wrinkle and skin whitening | |
| KR102373899B1 (en) | Multifunctional cosmetic composition containing complex natural extracts effective for skin wrinkle improvement, whitening, cell regeneration, and wound healing as an active ingredient | |
| CN110731918B (en) | Application of nitraria tangutorum bobr extract and skin external preparation containing nitraria tangutorum bobr extract | |
| Jang et al. | Antiaging activity of peptide identified from fermented Trapa Japonica fruit extract in human dermal fibroblasts | |
| JP2023171950A (en) | Anti-aging agent, antioxidant, anti-inflammatory agent, and whitening agent, as well as cosmetic | |
| KR20160054727A (en) | Ecklonia stolonifera hydrolysates that have high glucuronic acid cotent, preparation method thereof and antiaging cosmetic composition containing the same | |
| KR101587077B1 (en) | Composition containing fermentated opuntia humifusa showing biological activity of skin | |
| CN109966372A (en) | A kind of Cortex Lycii extract and its new opplication | |
| KR20140114240A (en) | The cosmetic composition for skin whitening | |
| KR20190018100A (en) | Cosmetic composition for Antioxidant, anti-wrinkle, anti-inflammatory, skin-whitening and hair-growing active compounds derived from Acorus Calamus Root extract | |
| CN109966191B (en) | Chrysanthemum flower extract and new application thereof | |
| KR20030047680A (en) | The Skin Lightening Cosmetic Composition Containing Extract Of Gardenia | |
| KR100416400B1 (en) | Composition containing extracts from dried roots of Trichosantes kirilowii maxim for external application and skin-whitening and process for preparation thereof | |
| KR20160128719A (en) | The cosmetic composition has a whitening effect of the active ingredients of physiologically algae | |
| CN109568205A (en) | Tyrosinase inhibitor and the thin skin water of whitening and preparation method thereof | |
| CN109966216B (en) | Chinese iris extract and new application thereof | |
| CN116120420A (en) | Bird's nest polypeptide composition, preparation method thereof and application thereof in anti-aging and whitening | |
| CN114437242B (en) | Usnic polysaccharide, preparation method and application thereof | |
| KR20170138822A (en) | A cosmetic composition for skin whitening |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 201403 Shanghai City, Fengxian District Lifenglu No. 12 Patentee after: Shanghai Natural Hall Group Co.,Ltd. Country or region after: China Address before: 201403 Shanghai City, Fengxian District Lifenglu No. 12 Patentee before: JALA Group Co. Country or region before: China |
|
| CP03 | Change of name, title or address |