CN110684679A - Biological bacterium for activating soil nutrient content and preparation method thereof - Google Patents
Biological bacterium for activating soil nutrient content and preparation method thereof Download PDFInfo
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- CN110684679A CN110684679A CN201911029314.3A CN201911029314A CN110684679A CN 110684679 A CN110684679 A CN 110684679A CN 201911029314 A CN201911029314 A CN 201911029314A CN 110684679 A CN110684679 A CN 110684679A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a biological bacterium for activating soil nutrient content and a preparation method thereof, wherein the biological bacterium comprises the following raw materials in parts by weight: 1-5 parts of aerobic microorganism mixed seed bacteria, 2-5 parts of saccharomycetes, 2-5 parts of lactic acid bacteria, 5-9 parts of molasses with the content of 10-15%, 1-5 parts of vibrio psychrophilus, 1-5 parts of bacillus psychrophilus and 2-5 parts of acinetobacter, and the invention also discloses a preparation method of the biological bacteria for activating the soil nutrient content, which are prepared by the invention, can ensure that various aerobic microorganisms can be rapidly fermented and propagated under the condition of sufficient oxygen by adding the aerobic microorganism mixed seed bacteria, obtain aerobic microorganism mixed bacteria, greatly reduce the inactivation rate of the aerobic microorganisms, and can better adapt to the low-temperature environment, rapidly propagate, accelerate the decomposition of organic matters, effectively activate the soil nutrients and benefit the growth of crops by adding the vibrio psychrophilus, the bacillus psychrophilus and the acinetobacter, the yield is improved.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a biological bacterium for activating soil nutrient content and a preparation method thereof.
Background
Soil nutrients are nutrient elements provided by soil that are essential for plant growth. Mineral nutrient components in the soil which can be directly absorbed by the root system of the plant or can be absorbed by the root system of the plant after transformation. Including 13 elements of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, iron, boron, molybdenum, zinc, manganese, copper, chlorine, etc. Nutrients are classified into major elements, medium elements and trace elements. In natural soil, it is mainly derived from soil minerals and soil organic matter, and secondly from atmospheric precipitation, slope water and groundwater. In cultivated soils, it also comes from fertilization and irrigation. The nutrient is divided into quick-acting nutrients and slow-acting nutrients according to the difficulty of plants in absorbing and utilizing nutrient elements. Generally, the available nutrients are present in only a small fraction, less than 1% of the total, and it should be noted that the partitioning of available nutrients and slow-acting nutrients is relative and both are always in dynamic equilibrium.
In many cultivated soils in China, the yield of crops is low due to low activity of soil microorganisms, low mineralization rate of organic substances and low nutrient availability, so that the development of a biological bacterium for activating the content of soil nutrients is urgently needed to overcome the defects in the current practical application.
Disclosure of Invention
The invention aims to provide a biological bacterium for activating soil nutrient content and a preparation method thereof, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a biological bacterium for activating soil nutrient content comprises the following raw materials in parts by weight: 1-5 parts of aerobic microorganism mixed seed bacteria, 2-5 parts of saccharomycetes, 2-5 parts of lactic acid bacteria, 5-9 parts of 10-15% molasses, 1-5 parts of vibrio psychrophilus, 1-5 parts of bacillus psychrophilus and 2-5 parts of acinetobacter.
As a further scheme of the invention: the feed comprises the following raw materials in parts by weight: 2-4 parts of aerobic microorganism mixed seed bacteria, 3-4 parts of saccharomycetes, 3-4 parts of lactic acid bacteria, 6-8 parts of 10-15% molasses, 2-4 parts of vibrio psychrophilus, 2-4 parts of bacillus psychrophilus and 3-4 parts of acinetobacter.
As a still further scheme of the invention: the feed comprises the following raw materials in parts by weight: 3 parts of aerobic microorganism mixed seed bacteria, 3.5 parts of saccharomycetes, 3.5 parts of lactic acid bacteria, 7 parts of 10-15% molasses, 3 parts of vibrio psychrophilus, 3 parts of bacillus psychrophilus and 3.5 parts of acinetobacter.
The preparation method of the biological bacteria for activating the soil nutrient content comprises the following steps:
1) respectively adding a plurality of aerobic microbial bacteria into the mixed culture medium for carrying out expanded culture for a plurality of days to obtain an expanded culture of each aerobic microbial bacteria, and equivalently mixing the expanded cultures of the various aerobic microbial bacteria to obtain aerobic microbial mixed seed bacteria;
2) respectively adding yeast and lactic acid bacteria into a liquid culture medium containing 10-15% of molasses to carry out amplification culture for several days to obtain a yeast culture solution and a lactic acid bacteria culture solution;
3) mixing aerobic microorganism mixed seed bacteria, a yeast culture solution and a lactic acid bacteria culture solution in proportion, inoculating the mixture to a nutrient gravy culture medium, and culturing the mixture for several days at constant temperature to obtain a mixed flora a;
4) proportionally mixing and inoculating the culture solution of the vibrio psychrophilus, the bacillus psychrophilus, the acinetobacter and the yeast, and culturing for several days at constant temperature to obtain a mixed flora b;
5) and compounding the mixed floras a and b to obtain a mixed floras c, and finally culturing to obtain the biological bacteria with the function of activating the soil nutrient content.
As a still further scheme of the invention: in the step 1) and the step 2), the days of the amplification culture are 5-7 days.
As a still further scheme of the invention: in the steps 3) and 4), the constant temperature culture temperature is 15 ℃.
As a still further scheme of the invention: in the step 3) and the step 4), the days of constant-temperature culture are not less than 7 days.
As a still further scheme of the invention: in step 5), the re-culturing step is: and (3) performing adaptive culture on the mixed flora c, performing passage in a 2-3L glass beaker, rotating speed of a stirring paddle at 400-500 rpm, culturing at 5-15 ℃, culturing for 3-5 days per generation, and culturing the mixed flora c for at least 20 generations to obtain the biological bacteria with the function of activating the soil nutrient content.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the biological bacteria for activating the soil nutrient content, the aerobic microorganism mixed seed bacteria are added, so that various aerobic microorganisms can be rapidly fermented and propagated under the sufficient oxygen condition, the aerobic microorganism mixed strain is obtained, and the inactivation rate of the aerobic microorganisms is greatly reduced;
2. by adding the vibrio psychrophilus, the bacillus psychrophilus and the acinetobacter, the biological bacteria can better adapt to a low-temperature environment, can be quickly propagated, can accelerate the decomposition of organic matters, effectively activate soil nutrients, are beneficial to the growth of crops and realize the improvement of the yield;
3. the mixed flora a and the mixed flora b are cultured in a step-by-step culture mode, so that the overall culture period of the biological bacteria can be shortened, and the number and the quality of effective viable bacteria of the biological bacteria can be improved.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to specific embodiments.
Example 1
A biological bacterium for activating soil nutrient content comprises the following raw materials in parts by weight: 1 part of aerobic microorganism mixed seed bacteria, 2 parts of saccharomycetes, 2 parts of lactic acid bacteria, 5 parts of 10-15% molasses, 1 part of vibrio psychrophilus, 1 part of bacillus psychrophilus and 2 parts of acinetobacter.
In this embodiment, the preparation method of the biological bacteria for activating soil nutrient content includes the following steps:
1) respectively adding various aerobic microbial bacteria into the mixed culture medium for carrying out expanded culture for 7 days to obtain expanded cultures of each aerobic microbial bacteria, and equivalently mixing the expanded cultures of the various aerobic microbial bacteria to obtain aerobic microbial mixed seed bacteria;
2) respectively adding yeast and lactic acid bacteria into a liquid culture medium containing 10-15% of molasses to carry out expanded culture for 7 days to obtain a yeast culture solution and a lactic acid bacteria culture solution;
3) mixing aerobic microorganism mixed seed bacteria, a yeast culture solution and a lactic acid bacteria culture solution according to a proportion, inoculating the mixture into a nutrient gravy culture medium, and culturing at constant temperature of 15 ℃ for 10 days to obtain mixed flora a;
4) proportionally mixing and inoculating the culture solution of the vibrio psychrophilus, the bacillus psychrophilus, the acinetobacter and the yeast, and culturing the mixture in a nutrient gravy culture medium at the constant temperature of 15 ℃ for 10 days to obtain a mixed flora b;
5) and compounding the mixed floras a and b to obtain a mixed floras c, carrying out adaptive culture on the mixed floras c, carrying out passage process in a 2L glass beaker, wherein the rotating speed of a stirring paddle is 400rpm, the culture temperature is 5 ℃, the culture time of each passage is 3 days, and obtaining the biological bacteria with the function of activating the soil nutrient content after the mixed floras c are cultured for at least 20 passages.
Example 2
A biological bacterium for activating soil nutrient content comprises the following raw materials in parts by weight: 5 parts of aerobic microorganism mixed seed bacteria, 5 parts of saccharomycetes, 5 parts of lactic acid bacteria, 9 parts of 10-15% molasses, 5 parts of vibrio psychrophilus, 5 parts of bacillus psychrophilus and 5 parts of acinetobacter.
In this embodiment, the preparation method of the biological bacteria for activating soil nutrient content includes the following steps:
1) respectively adding various aerobic microbial bacteria into the mixed culture medium for carrying out expanded culture for 5 days to obtain expanded cultures of each aerobic microbial bacteria, and equivalently mixing the expanded cultures of the various aerobic microbial bacteria to obtain aerobic microbial mixed seed bacteria;
2) respectively adding yeast and lactic acid bacteria into a liquid culture medium containing 10-15% of molasses to carry out expanded culture for 5 days to obtain a yeast culture solution and a lactic acid bacteria culture solution;
3) mixing aerobic microorganism mixed seed bacteria, a yeast culture solution and a lactic acid bacteria culture solution according to a proportion, inoculating the mixture into a nutrient gravy culture medium, and culturing at constant temperature of 15 ℃ for 7 days to obtain mixed flora a;
4) proportionally mixing and inoculating the culture solution of the vibrio psychrophilus, the bacillus psychrophilus, the acinetobacter and the yeast, and culturing the mixture in a nutrient gravy culture medium at the constant temperature of 15 ℃ for 7 days to obtain a mixed flora b;
5) and compounding the mixed floras a and b to obtain a mixed floras c, carrying out adaptive culture on the mixed floras c, carrying out passage in a 3L glass beaker, wherein the rotating speed of a stirring paddle is 500rpm, the culture temperature is 15 ℃, the culture time of each passage is 5 days, and obtaining the biological bacteria with the function of activating the soil nutrient content after the mixed floras c are cultured for at least 20 passages.
Example 3
A biological bacterium for activating soil nutrient content comprises the following raw materials in parts by weight: 2 parts of aerobic microorganism mixed seed bacteria, 3 parts of saccharomycetes, 3 parts of lactic acid bacteria, 6 parts of 10-15% molasses, 2 parts of vibrio psychrophilus, 2 parts of bacillus psychrophilus and 3 parts of acinetobacter.
In this embodiment, the preparation method of the biological bacteria for activating soil nutrient content includes the following steps:
1) respectively adding various aerobic microbial bacteria into the mixed culture medium for carrying out expanded culture for 6.5 days to obtain expanded cultures of each aerobic microbial bacteria, and mixing the expanded cultures of the various aerobic microbial bacteria in equal amount to obtain aerobic microbial mixed seed bacteria;
2) respectively adding yeast and lactic acid bacteria into a liquid culture medium containing 10-15% of molasses to carry out expanded culture for 6.5 days to obtain a yeast culture solution and a lactic acid bacteria culture solution;
3) mixing aerobic microorganism mixed seed bacteria, a yeast culture solution and a lactic acid bacteria culture solution according to a proportion, inoculating the mixture into a nutrient gravy culture medium, and culturing at a constant temperature of 15 ℃ for 9 days to obtain a mixed flora a;
4) proportionally mixing and inoculating the culture solution of the vibrio psychrophilus, the bacillus psychrophilus, the acinetobacter and the yeast, and culturing the mixture in a nutrient gravy culture medium at the constant temperature of 15 ℃ for 9 days to obtain a mixed flora b;
5) and compounding the mixed floras a and b to obtain a mixed floras c, carrying out adaptive culture on the mixed floras c, carrying out passage in a 3L glass beaker, wherein the rotating speed of a stirring paddle is 500rpm, the culture temperature is 15 ℃, the culture time of each passage is 5 days, and obtaining the biological bacteria with the function of activating the soil nutrient content after the mixed floras c are cultured for at least 20 passages.
Example 4
A biological bacterium for activating soil nutrient content comprises the following raw materials in parts by weight: 4 parts of aerobic microorganism mixed seed bacteria, 4 parts of saccharomycetes, 4 parts of lactic acid bacteria, 8 parts of 10-15% molasses, 4 parts of vibrio psychrophilus, 4 parts of bacillus psychrophilus and 4 parts of acinetobacter.
In this embodiment, the preparation method of the biological bacteria for activating soil nutrient content includes the following steps:
1) respectively adding various aerobic microbial bacteria into the mixed culture medium for carrying out expanded culture for 5.5 days to obtain expanded cultures of each aerobic microbial bacteria, and mixing the expanded cultures of the various aerobic microbial bacteria in equal amount to obtain aerobic microbial mixed seed bacteria;
2) respectively adding yeast and lactic acid bacteria into a liquid culture medium containing 10-15% of molasses to carry out expanded culture for 5.5 days to obtain a yeast culture solution and a lactic acid bacteria culture solution;
3) mixing aerobic microorganism mixed seed bacteria, a yeast culture solution and a lactic acid bacteria culture solution according to a proportion, inoculating the mixture into a nutrient gravy culture medium, and culturing at constant temperature of 15 ℃ for 8 days to obtain mixed flora a;
4) proportionally mixing and inoculating the culture solution of the vibrio psychrophilus, the bacillus psychrophilus, the acinetobacter and the yeast, and culturing the mixture in a nutrient gravy culture medium at the constant temperature of 15 ℃ for 8 days to obtain a mixed flora b;
5) and compounding the mixed floras a and b to obtain a mixed floras c, carrying out adaptive culture on the mixed floras c, carrying out passage in a 3L glass beaker, wherein the rotating speed of a stirring paddle is 500rpm, the culture temperature is 15 ℃, the culture time of each passage is 5 days, and obtaining the biological bacteria with the function of activating the soil nutrient content after the mixed floras c are cultured for at least 20 passages.
Example 5
A biological bacterium for activating soil nutrient content comprises the following raw materials in parts by weight: 3 parts of aerobic microorganism mixed seed bacteria, 3.5 parts of saccharomycetes, 3.5 parts of lactic acid bacteria, 7 parts of 10-15% molasses, 3 parts of vibrio psychrophilus, 3 parts of bacillus psychrophilus and 3.5 parts of acinetobacter.
In this embodiment, the preparation method of the biological bacteria for activating soil nutrient content includes the following steps:
1) respectively adding various aerobic microbial bacteria into the mixed culture medium for carrying out expanded culture for 6 days to obtain expanded cultures of each aerobic microbial bacteria, and equivalently mixing the expanded cultures of the various aerobic microbial bacteria to obtain aerobic microbial mixed seed bacteria;
2) respectively adding yeast and lactic acid bacteria into a liquid culture medium containing 10-15% of molasses to carry out expanded culture for 6 days to obtain a yeast culture solution and a lactic acid bacteria culture solution;
3) mixing aerobic microorganism mixed seed bacteria, a yeast culture solution and a lactic acid bacteria culture solution according to a proportion, inoculating the mixture into a nutrient gravy culture medium, and culturing at constant temperature of 15 ℃ for 8 days to obtain mixed flora a;
4) proportionally mixing and inoculating the culture solution of the vibrio psychrophilus, the bacillus psychrophilus, the acinetobacter and the yeast, and culturing the mixture in a nutrient gravy culture medium at the constant temperature of 15 ℃ for 8 days to obtain a mixed flora b;
5) and compounding the mixed floras a and b to obtain a mixed floras c, carrying out adaptive culture on the mixed floras c, carrying out passage in a 3L glass beaker, wherein the rotating speed of a stirring paddle is 500rpm, the culture temperature is 10 ℃, the culture time of each passage is 5 days, and obtaining the biological bacteria with the function of activating the soil nutrient content after the mixed floras c are cultured for at least 20 passages.
The biological bacteria for activating the soil nutrient content, which is prepared by the invention, can rapidly ferment and propagate various aerobic microbial bacteria under the condition of sufficient oxygen by adding the aerobic microbial mixed seed bacteria to obtain aerobic microbial mixed strains, so that the inactivation rate of the aerobic microbial bacteria is greatly reduced, the biological bacteria can better adapt to a low-temperature environment by adding the vibrio psychrophilus, the bacillus psychrophilus and the acinetobacter, the rapid propagation is realized, the decomposition of organic matters is accelerated, the soil nutrient is effectively activated, the growth of crops is facilitated, the yield is improved, the overall culture period of the biological bacteria can be shortened by adopting a step-by-step culture mode to culture the mixed flora a and the mixed flora b, and the number and the quality of effective viable bacteria of the biological bacteria can be improved.
While the preferred embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (8)
1. The biological bacteria for activating the soil nutrient content is characterized by comprising the following raw materials in parts by weight: 1-5 parts of aerobic microorganism mixed seed bacteria, 2-5 parts of saccharomycetes, 2-5 parts of lactic acid bacteria, 5-9 parts of 10-15% molasses, 1-5 parts of vibrio psychrophilus, 1-5 parts of bacillus psychrophilus and 2-5 parts of acinetobacter.
2. The biological bacteria for activating the soil nutrient content according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 2-4 parts of aerobic microorganism mixed seed bacteria, 3-4 parts of saccharomycetes, 3-4 parts of lactic acid bacteria, 6-8 parts of 10-15% molasses, 2-4 parts of vibrio psychrophilus, 2-4 parts of bacillus psychrophilus and 3-4 parts of acinetobacter.
3. The biological bacteria for activating the soil nutrient content according to claim 2, which is characterized by comprising the following raw materials in parts by weight: 3 parts of aerobic microorganism mixed seed bacteria, 3.5 parts of saccharomycetes, 3.5 parts of lactic acid bacteria, 7 parts of 10-15% molasses, 3 parts of vibrio psychrophilus, 3 parts of bacillus psychrophilus and 3.5 parts of acinetobacter.
4. A method for preparing biological bacteria for activating soil nutrient content according to any one of claims 1 to 3, which comprises the following steps:
1) respectively adding a plurality of aerobic microbial bacteria into the mixed culture medium for carrying out expanded culture for a plurality of days to obtain an expanded culture of each aerobic microbial bacteria, and equivalently mixing the expanded cultures of the various aerobic microbial bacteria to obtain aerobic microbial mixed seed bacteria;
2) respectively adding yeast and lactic acid bacteria into a liquid culture medium containing 10-15% of molasses to carry out amplification culture for several days to obtain a yeast culture solution and a lactic acid bacteria culture solution;
3) mixing aerobic microorganism mixed seed bacteria, a yeast culture solution and a lactic acid bacteria culture solution in proportion, inoculating the mixture to a nutrient gravy culture medium, and culturing the mixture for several days at constant temperature to obtain a mixed flora a;
4) proportionally mixing and inoculating the culture solution of the vibrio psychrophilus, the bacillus psychrophilus, the acinetobacter and the yeast, and culturing for several days at constant temperature to obtain a mixed flora b;
5) and compounding the mixed floras a and b to obtain a mixed floras c, and finally culturing to obtain the biological bacteria with the function of activating the soil nutrient content.
5. The method for preparing biological bacteria for activating soil nutrient content according to claim 4, wherein the number of days of the scale-up culture in the steps 1) and 2) is 5 to 7 days.
6. The method for preparing biological bacteria for activating soil nutrient content according to claim 4, wherein the constant temperature culture temperature in step 3) and step 4) is 15 ℃.
7. The method for preparing biological bacteria for activating soil nutrient content according to claim 6, wherein the days of constant temperature culture in the step 3) and the step 4) are not less than 7 days.
8. The method for preparing biological bacteria for activating soil nutrient content according to claim 7, wherein in the step 5), the re-culturing step is: and (3) performing adaptive culture on the mixed flora c, performing passage in a 2-3L glass beaker, rotating speed of a stirring paddle at 400-500 rpm, culturing at 5-15 ℃, culturing for 3-5 days per generation, and culturing the mixed flora c for at least 20 generations to obtain the biological bacteria with the function of activating the soil nutrient content.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911029314.3A CN110684679A (en) | 2019-10-28 | 2019-10-28 | Biological bacterium for activating soil nutrient content and preparation method thereof |
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| CN201911029314.3A CN110684679A (en) | 2019-10-28 | 2019-10-28 | Biological bacterium for activating soil nutrient content and preparation method thereof |
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| CN110684679A true CN110684679A (en) | 2020-01-14 |
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| CN201911029314.3A Withdrawn CN110684679A (en) | 2019-10-28 | 2019-10-28 | Biological bacterium for activating soil nutrient content and preparation method thereof |
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- 2019-10-28 CN CN201911029314.3A patent/CN110684679A/en not_active Withdrawn
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