CN110922242A - Microbial fertilizer suitable for improving soil matrix fertility in cold region barren environment - Google Patents
Microbial fertilizer suitable for improving soil matrix fertility in cold region barren environment Download PDFInfo
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- CN110922242A CN110922242A CN201911304167.6A CN201911304167A CN110922242A CN 110922242 A CN110922242 A CN 110922242A CN 201911304167 A CN201911304167 A CN 201911304167A CN 110922242 A CN110922242 A CN 110922242A
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- C05—FERTILISERS; MANUFACTURE THEREOF
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- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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Abstract
The invention discloses a microbial fertilizer suitable for improving soil matrix fertility in a cold region barren environment, which comprises the following raw materials in parts by weight: 28-32 parts of mushroom bran, 13-17 parts of bran coat, 3-5 parts of microbial agent, 3-5 parts of potash feldspar, 0.8-1.1 part of apatite and 4-6 parts of hornblende; the microbial agent consists of bradyrhizobium ehzense, rhizobium, arthrobacter psychrophilum, stenotrophomonas acidophilus, arthrobacter trophoblast, pantoea agglomerans and shortwave unicellular defect. The bacterial manure provided by the invention introduces functional microorganisms, promotes the construction of soil growth-promoting flora, promotes the rapid field planting of pioneer plants, and improves the success rate of ecological restoration of fresh geologic bodies.
Description
Technical Field
The invention belongs to the field of microbial fertilizers, and particularly relates to a microbial fertilizer formula suitable for improving the soil matrix fertility in a cold region barren environment.
Background
The existing bacterial fertilizer formula design is mostly aimed at the growth of agricultural crops, and the contained strains are single and similar and are generally bacillus, pseudomonas or azotobacter chroococcum and the like. The Chinese patent application with the application number of 201410837021.9 discloses a compound microbial fertilizer and application thereof, wherein the bacterial fertilizer comprises bacillus curvatus, pseudomonas and azotobacter chroococcum, and the application object is farmland vegetable soil which can promote the growth of leaf vegetables. The environment conditions required by the use of the existing bacterial manure are also superior, and the bacterial manure is generally a farmland environment with good temperature and humidity. Although the bacterial fertilizer product is suitable for constructing plant rhizosphere growth-promoting floras in farmland environment soil, the bacterial fertilizer product is difficult to be applied to promoting the growth of pioneer plants newly exposed to bare land in mountainous cold regions. In order to promote the rapid recovery of vegetation on bare land in cold regions, a new bacterial fertilizer formula suitable for improving the soil matrix fertility in the cold region barren environment needs to be designed according to the characteristics of the microbial functional group in the soil in the cold region.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: how to provide a microbial fertilizer which is suitable for improving the fertility of a soil matrix in a cold region barren environment.
The technical scheme of the invention is as follows: a microbial fertilizer suitable for improving soil matrix fertility in a cold region barren environment comprises the following raw materials in parts by weight: 28-32 parts of mushroom bran, 13-17 parts of bran coat, 3-5 parts of microbial agent, 3-5 parts of potash feldspar, 0.8-1.1 part of apatite and 4-6 parts of hornblende; the microbial agent consists of bradyrhizobium ehzense, rhizobium, arthrobacter psychrophilum, stenotrophomonas acidophilus, arthrobacter trophoblast, pantoea agglomerans and shortwave unicellular defect.
Furthermore, the ratio of bradyrhizobium ehmannii, rhizobia, arthrobacter psychrophilus, stenotrophomonas acidophilus, arthrobacter trophoblast, pantoea agglomerans and brevundimonas diminuta in the microbial agent is (1.4-2.2), (0.8-1.3), (3.5-4.4), (1.2-2.2), (0.5-1.0), (0.2-0.6), (0.1-0.4).
Further, the potash feldspar, the apatite and the hornblende are all granular substances which are crushed and then pass through a sieve with the aperture of 1 mm.
The invention also discloses a preparation method of the microbial fertilizer, which comprises the following steps:
(1) firstly, activating and propagating bradyrhizobium ehmannii, rhizobium, arthrobacter psychrophilum, stenotrophomonas acidophilus, arthrobacter trophoblast, pantoea agglomerans and shortwave monosperma strains;
(2) then slowly rhizobium ehrlichi, rhizobium, arthrobacter lacrimophilum, stenotrophomonas acidophilus, arthrobacter trophicus, pantoea agglomerans and shortwave monospermeoides are added into 10 milliliters of sterilized water according to the viable count proportion and are mixed evenly to prepare a microbial agent;
(3) weighing mushroom bran, bran coat, microbial agent, potash feldspar, apatite and amphibole according to the weight ratio, adjusting the water content to 40-50%, and uniformly mixing to obtain auxiliary materials;
(4) mixing the microbial inoculum obtained in the step (2) with the auxiliary material obtained in the step (3), fermenting at 10 ℃, turning over and introducing oxygen once every 2.5-3.5 days, and fermenting for 17-19 days; then adjusting the temperature to 18-22 ℃, fermenting, turning over the pile once every 2.5-3.5 days, and introducing oxygen, and fermenting for 17-19 days; then adjusting the temperature to 10 ℃ for fermentation, turning over the pile once every 2.5-3.5 days, and introducing oxygen for fermentation 17-19 days later; and (5) performing freeze-drying treatment to obtain the microbial fertilizer.
Compared with the prior art, the invention has the following beneficial effects:
in the past, bacterial manure formulas are mainly researched and developed according to the characteristics of soil microbial communities in agriculture and forestry, and traditional phosphorus-dissolving and nitrogen-fixing microorganisms such as bacillus, pseudomonas and azotobacter chroococcum are mostly used as core strain components of the bacterial manure formulas. The bacterial manure formula is different from the previous formula, is developed based on glacier recession land barren soil in which vegetation primary succession is taking place, and takes representative functional microorganisms such as bradyrhizobium ehzekii, rhizobium, psychrophilic lactose arthrobacter, stenotrophomonas acidophilus, arthrobacter trophicus, pantoea agglomerans and shortwave unicellular which can be cultured and can promote vegetation planting as core strain components. The bacterial manure can be used for improving the soil body matrix fertility of disaster lands and engineering damaged lands in high-altitude areas and promoting the field planting recovery of plants.
Detailed Description
The bradyrhizobium ehmannii, rhizobium, arthrobacter lachnophilum, stenotrophomonas acidophilus, arthrobacter trophoblast, pantoea agglomerans and shortwave monospermum can be ordered from a strain preservation center of North Nay biology company (website http:// www.bnbio.com/default. htm): wherein the accession number of the Bradyrhizobium ehrlichiae (Bradyrhizobium elkanii) is BNCC 170472; the accession number of Rhizobium (Rhizobium leguminosarum) is BNCC 133833; arthrobacter psychrophilus (Arthrobacter psychrolophyllus) accession number BNCC 139149; stenotrophomonas acidophilus (Stenotrophomonas acidophilus) with accession number BNCC 157124; the accession number of the Arthrobacter trophoblast (Arthrobacterpascens) is BNCC 222140; the collection number of the Pantoea agglomerans is BNCC 221876; shortwave monimonas diminuta (Brevundimonas diminuta) accession number BNCC 167154.
Example 1
The microbial inoculation agent comprises the following strains: bradyrhizobium ehmannii, rhizobia, arthrobacter psychrophilus, stenotrophomonas acidophilus, arthrobacter trophoblast, pantoea agglomerans and shortwave monosomyia defectively;
the microbial strains can be ordered from strain preservation center (website) of Beinanbio corporationhttp:// www.bnbio.com/default.htm)；
The activation and propagation method of the strain comprises the following steps: according to the order specification method;
the microorganism inoculation microbial inoculum comprises bradyrhizobium ehzedii, rhizobium, arthrobacter psychrophilum, stenotrophomonas acidophilus, arthrobacter trophicus, pantoea agglomerans and shortwave monosomyia defectively in viable count proportion: 1.4:0.8:3.5:1.2:0.5:0.2:0.1, adding into 10 ml of sterilized water, and uniformly mixing to prepare a microbial inoculation agent;
weighing the following raw materials in parts by weight: 28 parts of mushroom bran, 13 parts of bran coat, 3 parts of potash feldspar, 0.8 part of apatite and 4 parts of amphibole, adjusting the water content to 45%, and uniformly mixing to prepare auxiliary materials; the potash feldspar, the apatite and the hornblende are all granular substances which are crushed and then screened by a sieve with the aperture of 1 mm;
mixing the microbial inoculum with adjuvants, fermenting at 10 deg.C, turning over and introducing oxygen once every 3 days, and fermenting for 18 days; then adjusting the temperature to 20 ℃, fermenting, turning over the pile once every 3 days, and introducing oxygen for 18 days; then adjusting the temperature to 10 ℃ for fermentation, turning over the pile once every 3 days, and introducing oxygen for 18 days; and (5) performing freeze-drying treatment to obtain the microbial fertilizer.
Example 2
The microbial inoculation agent comprises the following strains: bradyrhizobium ehmannii, rhizobia, arthrobacter psychrophilus, stenotrophomonas acidophilus, arthrobacter trophoblast, pantoea agglomerans and shortwave monosomyia defectively;
the microbial strains can be ordered from strain preservation center (website) of Beinanbio corporationhttp:// www.bnbio.com/default.htm)；
The activation and propagation method of the strain comprises the following steps: according to the order specification method;
the microorganism inoculation microbial inoculum comprises bradyrhizobium ehzedii, rhizobium, arthrobacter psychrophilum, stenotrophomonas acidophilus, arthrobacter trophicus, pantoea agglomerans and shortwave monosomyia defectively in viable count proportion: 2.2:1.3:4.4:2.2:1.0:0.6:0.4, adding into 10 ml of sterilized water, and uniformly mixing to prepare a microbial inoculation agent;
weighing the following raw materials in parts by weight: 32 parts of mushroom bran, 17 parts of bran coat, 5 parts of potash feldspar, 1.1 parts of apatite and 6 parts of amphibole, adjusting the water content to 55%, and uniformly mixing to obtain an auxiliary material 2; the potash feldspar, the apatite and the hornblende are all granular substances which are crushed and then screened by a sieve with the aperture of 1 mm;
mixing the microbial inoculum with adjuvants, fermenting at 10 deg.C, turning over and introducing oxygen once every 3 days, and fermenting for 18 days; then adjusting the temperature to 20 ℃, fermenting, turning over the pile once every 3 days, and introducing oxygen for 18 days; then adjusting the temperature to 10 ℃ for fermentation, turning over the pile once every 3 days, and introducing oxygen for 18 days; and (5) performing freeze-drying treatment to obtain the microbial fertilizer.
Experimental example 1
In 2016, indoor bacterial manure tests are carried out between 6 months and 8 months, matrixes of a test group and a control group are granite scraps, the test group is applied with the microbial manure prepared in the example 1 and sowed with astragalus seeds, and the control group is sowed with the astragalus seeds only. The test temperature is set to 15 ℃, the water content is set to 20%, and after 50 days, effect comparison is carried out: the effective nitrogen and phosphorus concentration of the test group is more than 10 times of that of the control group, and the astragalus biomass of the test group is about 4 times higher than that of the control group.
Experimental example 2
In 2017, field bacterial manure tests are carried out on mountain debris flow lands between 6 months and 9 months, the matrixes of the test group and the control group are barren debris of debris flow, the microbial manure prepared in example 2 is applied to the test group, the sea buckthorn seedlings are transplanted, and only the sea buckthorn seedlings are transplanted to the control group. After 65 days of the test, the effect comparison is carried out: the effective nitrogen and phosphorus concentration of the test group is more than 3 times of that of the control group, and the survival rate of the sea buckthorn of the test group is about 3.5 times higher than that of the control group.
The above-mentioned embodiments only express the specific embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present application. It should be noted that, for those skilled in the art, without departing from the technical idea of the present application, several changes and modifications can be made, which are all within the protection scope of the present application.
Claims (4)
1. The microbial fertilizer for improving the fertility of a soil matrix in a cold region barren environment is characterized by comprising the following raw materials in parts by weight: 28-32 parts of mushroom bran, 13-17 parts of bran coat, 3-5 parts of microbial agent, 3-5 parts of potash feldspar, 0.8-1.1 part of apatite and 4-6 parts of hornblende; the microbial agent consists of bradyrhizobium ehzense, rhizobium, arthrobacter psychrophilum, stenotrophomonas acidophilus, arthrobacter trophoblast, pantoea agglomerans and shortwave unicellular defect.
2. The microbial fertilizer according to claim 1, wherein the microbial agent comprises bradyrhizobium ehmannii, rhizobium, Arthrobacter psychrophilus, stenotrophomonas acidophilus, Arthrobacter trophicus, Pantoea agglomerans and Brevundimonas diminuta in a ratio of (1.4-2.2) to (0.8-1.3) to (3.5-4.4) to (1.2-2.2) to (0.5-1.0) to (0.2-0.6) to (0.1-0.4).
3. The microbial fertilizer according to claim 1, wherein the potash feldspar, the apatite and the amphibole are granular substances which are crushed and then sieved through a sieve with the aperture of 1 mm.
4. The method for preparing a microbial fertilizer according to any one of claims 1 to 3, comprising the steps of:
(1) firstly, activating and propagating bradyrhizobium ehmannii, rhizobium, arthrobacter psychrophilum, stenotrophomonas acidophilus, arthrobacter trophoblast, pantoea agglomerans and shortwave monosperma strains;
(2) then slowly rhizobium ehrlichi, rhizobium, arthrobacter lacrimophilum, stenotrophomonas acidophilus, arthrobacter trophicus, pantoea agglomerans and shortwave monospermeoides are added into 10 milliliters of sterilized water according to the viable count proportion and are mixed evenly to prepare a microbial agent;
(3) weighing mushroom bran, bran coat, microbial agent, potash feldspar, apatite and amphibole according to the weight ratio, adjusting the water content to 40-50%, and uniformly mixing to obtain auxiliary materials;
(4) mixing the microbial inoculum obtained in the step (2) with the auxiliary material obtained in the step (3), fermenting at 10 ℃, turning over and introducing oxygen once every 2.5-3.5 days, and fermenting for 17-19 days; then adjusting the temperature to 18-22 ℃, fermenting, turning over the pile once every 2.5-3.5 days, and introducing oxygen, and fermenting for 17-19 days; then adjusting the temperature to 10 ℃ for fermentation, turning over the pile once every 2.5-3.5 days, and introducing oxygen for fermentation 17-19 days later; and (5) performing freeze-drying treatment to obtain the microbial fertilizer.
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| WO2022057299A1 (en) * | 2020-09-18 | 2022-03-24 | 南京林业大学 | Phosphate-solubilizing and growth-promoting bacterium (arthrobacter pascens x-1) for promoting growth of root nodule and improving abundance of probiotic microorganism population, and application thereof |
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Application publication date: 20200327 |