CN110680932A - Non-destructive degerming process for hyaluronic acid aqueous solution - Google Patents
Non-destructive degerming process for hyaluronic acid aqueous solution Download PDFInfo
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- CN110680932A CN110680932A CN201911091437.XA CN201911091437A CN110680932A CN 110680932 A CN110680932 A CN 110680932A CN 201911091437 A CN201911091437 A CN 201911091437A CN 110680932 A CN110680932 A CN 110680932A
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- Prior art keywords
- hyaluronic acid
- aqueous solution
- destructive
- meshes
- acid aqueous
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/022—Filtration
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/20—Targets to be treated
- A61L2202/21—Pharmaceuticals, e.g. medicaments, artificial body parts
Abstract
The invention relates to a hyaluronic acid process, in particular to a non-destructive degerming process of a hyaluronic acid aqueous solution. According to the non-destructive degerming process of the hyaluronic acid aqueous solution, hyaluronic acid is prepared into the aqueous solution according to the concentration of the formula, then the aqueous solution is fully stirred and uniformly mixed, the aqueous solution is kept stand overnight to be fully dissolved, the solution sequentially passes through screens of 400 meshes, 1000 meshes and 2000 meshes, and then sequentially passes through filter membranes of 2um,1um,0.45um and 0.22um after high-temperature sterilization in a super-clean workbench in a pressurized state so as to achieve the purpose of final physical degerming. The non-destructive degerming process of the hyaluronic acid aqueous solution can effectively remove microorganisms in the hyaluronic acid aqueous solution without any risk of residues of carcinogenic substances and other harmful chemical substances, and is particularly suitable for the production of organic skin care and cosmetics without addition of substances.
Description
Technical Field
The invention relates to external cosmetics, skin care products and therapeutic liniment based on hyaluronic acid aqueous solution, and hyaluronic acid aqueous solution for injection, in particular to a nondestructive degerming process of hyaluronic acid aqueous solution.
Background
At present, the aqueous solution of hyaluronic acid is widely used for preparing various cosmetics and skin care products, and because microorganisms with the nutritional characteristics of hyaluronic acid are easy to breed in the aqueous solution, the antibacterial and antiseptic properties become a great consideration factor for preparing hyaluronic acid products. Hyaluronic acid is biologically active, and is often present in aqueous solutions in the form of long-chain polymers, and some of its activity is related to the length of the polymer. Autoclaving can lead to polymer fragmentation and the destructive degradation of the hyaluronic acid molecule, and is not suitable for such products.
Currently, the commonly used antibacterial and antiseptic means is to add chemical antiseptic or fumigate with ethylene oxide.
The existing hyaluronic acid antiseptic and antibacterial technology basically takes ethylene oxide fumigation and chemical preservatives as main components. However, this class of technologies has a non-green environmental and potential health hazard. Ethylene oxide is a strong carcinogen and even under adequate post-fumigation ventilation conditions, complete absence of ethylene oxide residue in the product is not guaranteed. Various chemical preservatives also pose potential carcinogenic risks as well as chemical residue problems. The conventional physical sterilization technology such as autoclaving and radiation irradiation can seriously damage the physicochemical and biological properties of the hyaluronic acid aqueous solution, such as seriously reduced viscosity, reduced molecular weight and lost bioactivity. And the conventional filtration sterilization is easy to cause the filter membrane to be blocked due to the high viscosity of the hyaluronic acid solution, and has poor practicability.
Disclosure of Invention
Aiming at the defects in the prior art, the non-destructive degerming process for the hyaluronic acid aqueous solution solves the technical problems, and provides a physical degerming method for treating the hyaluronic acid solution, and the non-destructive degerming process for the hyaluronic acid aqueous solution, which maintains various physicochemical and biological properties of the hyaluronic acid solution.
In order to achieve the purpose, the invention provides the following technical scheme:
the non-destructive degerming process of the hyaluronic acid aqueous solution comprises the following steps:
step one, preparing hyaluronic acid into an aqueous solution according to the concentration of a formula, fully stirring and uniformly mixing, and standing for 12-24 hours to fully dissolve hyaluronic acid;
step two, the dissolved solution passes through screens of 400 meshes, 1000 meshes and 2000 meshes in sequence;
filtering by a screen, placing the filter membrane in an aseptic workbench, and sequentially passing through filter membranes of 2um,1um,0.45um and 0.22um subjected to high-temperature sterilization in a pressurizing manner;
and step four, filtering the mixture by a filter membrane for later use.
Wherein, the hyaluronic acid solution comprises the following components in percentage: 0.2-1% of hyaluronic acid; 0.5-5% of glycerin; a proper amount of water; 5mM to 20mM of sodium phosphate buffer solution.
Wherein, the hyaluronic acid solution comprises the following components in percentage: 0.2% of hyaluronic acid; 0.5% of glycerin; a proper amount of water; sodium phosphate buffer 5 mM.
Wherein, the hyaluronic acid solution comprises the following components in percentage: 0.5% of hyaluronic acid; 5% of glycerol; a proper amount of water; sodium phosphate buffer 10 mM.
Wherein, the hyaluronic acid solution comprises the following components in percentage: 1% of hyaluronic acid; 3% of glycerol; a proper amount of water; sodium phosphate buffer 20 mM.
Wherein the pH value of the sodium phosphate buffer solution is 6.
The invention has the beneficial effects that: the non-destructive degerming process for the hyaluronic acid aqueous solution solves the problem of the degerming process for the hyaluronic acid aqueous solution by multi-step and multi-aperture filtration, can effectively remove microorganisms in the hyaluronic acid aqueous solution, and does not have any risk of residual carcinogenic substances and other harmful chemical substances. Is especially suitable for the production of organic and additive-free skin care and cosmetic.
For a better understanding of the features and technical content of the present invention, refer to the following detailed description of the invention.
Detailed Description
To further illustrate the technical means adopted by the present invention and the effects thereof, the following detailed description is given with reference to the preferred embodiments of the present invention.
The non-destructive degerming process of the hyaluronic acid aqueous solution comprises the following steps:
step one, preparing hyaluronic acid into an aqueous solution according to the concentration of a formula, fully stirring and uniformly mixing, and standing for 12-24 hours to fully dissolve hyaluronic acid;
step two, the dissolved solution passes through screens of 400 meshes, 1000 meshes and 2000 meshes in sequence;
filtering by a screen, placing the filter membrane in an aseptic workbench, and sequentially passing through filter membranes of 2um,1um,0.45um and 0.22um subjected to high-temperature sterilization in a pressurizing manner;
and step four, filtering the mixture by a filter membrane for later use.
Specifically, the hyaluronic acid solution comprises the following components in percentage: 0.2-1% of hyaluronic acid; 0.5-5% of glycerin; a proper amount of water; sodium phosphate buffer 5 mM-20 mM (millimole per liter).
Example 1
The hyaluronic acid solution comprises the following components in percentage: 0.2% of hyaluronic acid; 0.5% of glycerin; a proper amount of water; sodium phosphate buffer 5 mM.
Step one, preparing hyaluronic acid into an aqueous solution according to the concentration of a formula, fully stirring and uniformly mixing, and standing for 12 hours to fully dissolve hyaluronic acid;
step two, the dissolved solution passes through screens of 400 meshes, 1000 meshes and 2000 meshes in sequence;
filtering by a screen, placing the filter membrane in an aseptic workbench, and sequentially passing through filter membranes of 2um,1um,0.45um and 0.22um subjected to high-temperature sterilization in a pressurizing manner;
and step four, filtering the mixture by a filter membrane for later use.
Example 2
The hyaluronic acid solution comprises the following components in percentage: 0.5% of hyaluronic acid; 5% of glycerol; a proper amount of water; sodium phosphate buffer 10 mM.
Step one, preparing hyaluronic acid into an aqueous solution according to the concentration of a formula, fully stirring and uniformly mixing, and standing for 16 hours to fully dissolve hyaluronic acid;
step two, the dissolved solution passes through screens of 400 meshes, 1000 meshes and 2000 meshes in sequence;
filtering by a screen, placing the filter membrane in an aseptic workbench, and sequentially passing through filter membranes of 2um,1um,0.45um and 0.22um subjected to high-temperature sterilization in a pressurizing manner;
and step four, filtering the mixture by a filter membrane for later use.
Example 3
The hyaluronic acid solution comprises the following components in percentage: 1% of hyaluronic acid; 3% of glycerol; a proper amount of water; sodium phosphate buffer 20 mM.
Step one, preparing hyaluronic acid into an aqueous solution according to the concentration of a formula, fully stirring and uniformly mixing, and standing for 24 hours to fully dissolve hyaluronic acid;
step two, the dissolved solution passes through screens of 400 meshes, 1000 meshes and 2000 meshes in sequence;
filtering by a screen, placing the filter membrane in an aseptic workbench, and sequentially passing through filter membranes of 2um,1um,0.45um and 0.22um subjected to high-temperature sterilization in a pressurizing manner;
and step four, filtering the mixture by a filter membrane for later use.
Specifically, the pH value of the sodium phosphate buffer solution is 6.
The hyaluronic acid aqueous solution can be used for the production of various organic and additive-free skin care and cosmetics, and the effects of the skin care and cosmetics are improved.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (6)
1. The non-destructive degerming process of the hyaluronic acid aqueous solution is characterized by comprising the following steps of:
step one, preparing hyaluronic acid into an aqueous solution according to the concentration of a formula, fully stirring and uniformly mixing, and standing for 12-24 hours to fully dissolve hyaluronic acid;
step two, the dissolved solution passes through screens of 400 meshes, 1000 meshes and 2000 meshes in sequence;
filtering by a screen, placing the filter membrane in an aseptic workbench, and sequentially passing through filter membranes of 2um,1um,0.45um and 0.22um subjected to high-temperature sterilization in a pressurizing manner;
and step four, filtering the mixture by a filter membrane for later use.
2. The non-destructive degerming process of hyaluronic acid aqueous solution according to claim 1, characterized in that it comprises, in percentages: 0.2-1% of hyaluronic acid; 0.5-5% of glycerin; a proper amount of water; 5mM to 20mM of sodium phosphate buffer solution.
3. The non-destructive degerming process of hyaluronic acid aqueous solution according to claim 2, characterized in that it comprises, in percentages: 0.2% of hyaluronic acid; 0.5% of glycerin; a proper amount of water; sodium phosphate buffer 5 mM.
4. The non-destructive degerming process of hyaluronic acid aqueous solution according to claim 1, characterized in that it comprises, in percentages: 0.5% of hyaluronic acid; 5% of glycerol; a proper amount of water; sodium phosphate buffer 10 mM.
5. The non-destructive degerming process of hyaluronic acid aqueous solution according to claim 1, characterized in that it comprises, in percentages: 1% of hyaluronic acid; 3% of glycerol; a proper amount of water; sodium phosphate buffer 20 mM.
6. The non-destructive sterilization process of an aqueous hyaluronic acid solution according to claim 1, wherein said sodium phosphate buffer has a pH of 6.
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| CN201911091437.XA CN110680932A (en) | 2019-11-10 | 2019-11-10 | Non-destructive degerming process for hyaluronic acid aqueous solution |
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| CN201911091437.XA CN110680932A (en) | 2019-11-10 | 2019-11-10 | Non-destructive degerming process for hyaluronic acid aqueous solution |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008266524A (en) * | 2007-04-24 | 2008-11-06 | Hisamitsu Pharmaceut Co Inc | Method for sterilizing chemical fluid, pre-filled syringe and method for producing the same |
| CN102516411A (en) * | 2011-11-30 | 2012-06-27 | 上海景峰制药有限公司 | Method for pre-treating sodium hyaluronate fermentation broth |
| CN106397630A (en) * | 2016-08-31 | 2017-02-15 | 新疆阜丰生物科技有限公司 | Method for extracting sodium hyaluronate based on membrane separation technology |
| CN107149688A (en) * | 2017-04-27 | 2017-09-12 | 南京华开生物科技有限公司 | The sterile production method of hyalomitome acids product |
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2019
- 2019-11-10 CN CN201911091437.XA patent/CN110680932A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008266524A (en) * | 2007-04-24 | 2008-11-06 | Hisamitsu Pharmaceut Co Inc | Method for sterilizing chemical fluid, pre-filled syringe and method for producing the same |
| CN102516411A (en) * | 2011-11-30 | 2012-06-27 | 上海景峰制药有限公司 | Method for pre-treating sodium hyaluronate fermentation broth |
| CN106397630A (en) * | 2016-08-31 | 2017-02-15 | 新疆阜丰生物科技有限公司 | Method for extracting sodium hyaluronate based on membrane separation technology |
| CN107149688A (en) * | 2017-04-27 | 2017-09-12 | 南京华开生物科技有限公司 | The sterile production method of hyalomitome acids product |
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Application publication date: 20200114 |