CN116036357A - Protein liquid dressing for laser and photon postoperative repair and preparation method thereof - Google Patents
Protein liquid dressing for laser and photon postoperative repair and preparation method thereof Download PDFInfo
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- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0019—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
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Abstract
The invention relates to a protein liquid dressing for laser and photon postoperative repair and a preparation method thereof. The invention relates to a protein liquid dressing for laser and photon postoperative repair, which comprises the following components in parts by weight: recombinant type III humanized collagen: 0.5wt% to 1.5wt%; mussel mucin is: 0.5wt% to 1.5wt%; fibroin peptide: 0.1wt% to 1.0wt%; polyethylene glycol: 1.0wt% to 5.0wt%; purified water: 91.0wt% to 97.9wt%. Under the synergistic effect of recombinant III type humanized collagen, mussel mucin, fibroin peptide, polyethylene glycol and purified water, on one hand, the collagen can be directly supplemented for wounds, the wound healing after laser and photon operations is promoted, the formation of scars and pigmentation is avoided, the effects of relieving itching, pain and redness on the wounds are achieved, and the user experience of the product is greatly improved; on the other hand, by promoting collagen synthesis, activating cells, accelerating wound healing, avoiding scar and pigmentation formation, and simultaneously playing a role in postoperative water supplementing.
Description
Technical Field
The invention relates to the field of medical cosmetology, in particular to a protein liquid dressing for laser and photon postoperative repair and a preparation method thereof.
Background
Collagen is present in the outer matrix of our skin cells, and it accounts for about 80% of the outer matrix of the skin. The human body needs to update 10% of collagen every day, and the collagen is very important for healing and repairing the skin.
Collagen commonly used in skin care products is mainly of animal origin, for example, extracted from animal tissues such as pig skin, bovine Achilles tendon, fish skin and the like, is heterologous collagen, has immune rejection reaction to human body, and can carry virus risks such as swine influenza, mad cow disease, swine erysipelas and the like. The application of heterologous collagen to humans generally presents a low efficiency of use and a high risk of use. The recombinant collagen is humanized collagen obtained by a genetic engineering technology, has no immunological rejection reaction to human bodies, has no risk of carrying viruses, and is very friendly to human skin. The recombinant collagen can promote regeneration of myoglobin, promote proliferation and adhesion of cells, effectively improve microenvironment of skin cells and condition skin.
The wound after laser and photon operation needs to be supplemented with collagen, and burning, pain and itching can occur in the repairing process after laser and photon operation, and the problems of scar and pigmentation can also be caused.
Disclosure of Invention
Based on the above, the invention aims to overcome the defects of the prior art and provide a protein liquid dressing for laser and photon postoperative repair and a preparation method thereof, wherein the protein liquid dressing for laser and photon postoperative repair has good safety, can be used for laser and photon postoperative repair, and can promote wound healing, reduce wound scars and pigmentation; reduce burning sensation, pain sensation and itching after laser and photon operation.
A protein liquid dressing for laser and photon postoperative repair, which comprises the following components in parts by weight:
recombinant type III humanized collagen: 0.5wt% to 1.5wt%;
mussel mucin: 0.5wt% to 1.5wt%;
fibroin peptide: 0.1wt% to 1.0wt%;
polyethylene glycol: 1.0wt% to 5.0wt%;
purified water: 91.0wt% to 97.9wt%.
Recombinant III type humanized collagen, which is homologous to human collagen, has consistent structure, no allergy and no stimulation; the production raw materials are non-animal sources, animal source impurities are not existed, and immune response is avoided; the protein produced by the genetically engineered bacteria fundamentally overcomes the hidden trouble of animal viruses, and has very safe sources. The molecular weight and the structure of the skin care gel are very similar to those of human collagen, and the skin care gel is just like the human collagen, so that the skin care gel has the advantages of good water solubility, high activity and good skin permeability, and can effectively promote skin healing.
Mussel mucin is derived from the podophylloids of marine organism Mytilus edulis, and is a high-purity single protein which is easy to be absorbed by human bodies. Because all cells of the human body are negatively charged and mussel mucin is positively charged, the negatively charged cells must climb in the positively charged direction within the range covered by the mussel mucin, so the repair speed of the mussel mucin is many times faster than the self-repair speed of the skin, and the effect of rapidly repairing the skin barrier is further realized. Mussel mucin can also obviously relieve itching after burn, promote healing of wound surfaces after laser and photon operations, be used for repairing wound surfaces after microplasma treatment of acne scar, and relieve adverse reactions such as erythema, edema, desquamation, pain, itching and the like caused by laser and photon treatment.
Fibroin peptide is a polypeptide substance that is water-soluble by degradation of fibroin. The fibroin peptide can permeate the dermis layer of the skin, promote collagen synthesis, activate cells, improve the immunity of the cells, promote the metabolism rate of the skin and help repair damaged skin tissues. The fibroin peptide has good curative effects on chapped skin, inflammation, itching and chloasma, can accelerate wound healing, and can prevent invasion and injury of chemical substances such as dust, plastics, acid, alkali and the like to skin.
According to the protein liquid dressing for laser and photon postoperative repair, under the synergistic effect of the recombinant III-type humanized collagen, mussel mucin, fibroin peptide, polyethylene glycol and purified water, on one hand, collagen can be directly supplemented to wounds, the laser and photon postoperative wound healing is promoted, the formation of scars and pigmentation is avoided, the effects of relieving itching, pain and redness on the wounds are achieved, and the user experience of products is greatly improved; on the other hand, by promoting collagen synthesis, activating cells, accelerating wound healing, avoiding scar and pigmentation formation, and simultaneously playing a role in postoperative water supplementing. In the protein liquid dressing for laser and photon postoperative repair, the content of recombinant III type humanized collagen and mussel mucin is 0.5-1.5 wt% and the content of fibroin peptide is 0.1-1.0 wt%; if the content of the recombinant III type humanized collagen and fibroin peptide is too low, the scar and pigmentation of the wound are not improved, and a synergistic effect is difficult to form among the components of the product; if the content of the recombinant III type humanized collagen and fibroin peptide is too high, the effect of the product can not be greatly improved; in the protein liquid dressing for laser and photon postoperative repair, the content of mussel mucin is 0.5-1.5 wt%; if the content of mussel mucin is too low, the burn, pain and itching after operation are not easy to be relieved, and the synergistic effect among the components of the product is difficult to be formed; if the content of mussel mucin is too high, the effect cannot be greatly improved.
Further, the content of the recombinant type III humanized collagen is 1wt%.
Further, the mussel mucin content is 1wt%.
Further, the content of the fibroin peptide is 0.5wt%.
Further, the content of the polyethylene glycol is 2wt%.
Further, the purified water content was 95.5wt%.
Further, the polyethylene glycol is polyethylene glycol 400. The polyethylene glycol 400 has moderate molecular weight and can be used as a thickening agent to increase the viscosity of the protein liquid dressing for laser and photon postoperative repair, so that the protein liquid dressing for laser and photon postoperative repair can be conveniently applied to a wound of the laser and photon. Meanwhile, the polyethylene glycol 400 is used as a humectant, and can be used for supplementing water for wounds after laser and photon operations.
The invention also provides a preparation method of the protein liquid dressing for laser and photon postoperative repair, which comprises the following steps:
weighing the components according to the proportion, and then mixing and dissolving polyethylene glycol and water at room temperature to prepare a solution; mixing the recombinant III type humanized collagen, mussel mucin and fibroin peptide with the solution, uniformly stirring, and carrying out irradiation sterilization to obtain any protein liquid dressing for laser and photon postoperative repair.
The preparation method of the protein liquid dressing for laser and photon postoperative repair is simple in steps and can be operated under normal temperature.
Further, a homogenizing mixer is used for stirring during the stirring process. And (3) fully and uniformly mixing the recombinant III-type humanized collagen, mussel mucin and fibroin peptide with the solution by adopting a homogenizing mixer.
Further, the solution was vacuum defoamed during stirring. The vacuum defoaming treatment can eliminate foam, reduce the harmful influence of the foam, and improve the quality of the prepared protein liquid dressing for laser and photon postoperative repair.
Further, the irradiation sterilization is cobalt 60 irradiation sterilization. According to the invention, the protein liquid dressing for laser and photon postoperative repair is prepared by cobalt 60 irradiation sterilization without adding preservative, so that the safety of the prepared protein liquid dressing for laser and photon postoperative repair is greatly improved.
The invention also provides application of the protein liquid dressing for laser and photon postoperative repair.
The protein liquid dressing for laser and photon postoperative repair can not promote wound healing and reduce wound scar and pigmentation under the synergistic effect of recombinant III type humanized collagen, mussel mucin, fibroin peptide, polyethylene glycol and purified water; the burn, pain and itching after laser and photon operations can be reduced simultaneously, and the water supplementing device is used for supplementing water after laser and photon operations. The protein liquid dressing for laser and photon postoperative repair can be used for repairing a wound surface after laser and photon operation, and the product is in a liquid form, so that the protein liquid dressing greatly meets the requirements of consumers.
For a better understanding and implementation, the present invention is described in detail below with reference to the drawings.
Drawings
FIG. 1 is a morphology of the cell viability status of the protein liquid dressing for laser, photon post-operative repair of example 1 for in vitro cytotoxicity evaluation;
FIG. 2 shows the cell viability of the liquid protein dressings of examples 1-4 for laser, photon post-operative repair for in vitro cytotoxicity evaluation.
Detailed Description
The invention is further described below with reference to the accompanying drawings.
In the following examples and comparative examples, the experimental methods used were conventional methods unless otherwise specified, and the materials, reagents and the like used, unless otherwise specified, were all commercially available. The recombinant type III humanized collagen source Jiangsu Jiangshan Congress Biotechnology Co., ltd was used in the following examples.
Example 1
A protein liquid dressing for laser and photon postoperative repair, which comprises the following components in parts by weight:
1g of recombinant III type humanized collagen, 1g of mussel mucin, 0.5g of fibroin peptide, 400 g of polyethylene glycol 2g and 95.5g of purified water.
The preparation method of the protein liquid dressing for laser and photon postoperative repair comprises the following steps:
weighing 2g of polyethylene glycol 400, 95.5g of purified water, 1g of recombinant type III humanized collagen, 1g of mussel mucin and 0.5g of fibroin peptide according to the proportion; then, 2g of polyethylene glycol 400 and 95.5g of purified water were mixed and dissolved at room temperature to prepare a solution. Mixing 1g of recombinant III-type humanized collagen, 1g of mussel mucin and 0.5g of fibroin peptide with the above solution, uniformly stirring, and sterilizing by cobalt 60 irradiation to obtain the protein liquid dressing for laser and photon postoperative repair. Wherein, in the stirring process, a homogenizing stirrer is used for fully and uniformly mixing the solution, and the solution is subjected to vacuum defoaming.
Example 2
A protein liquid dressing for laser and photon postoperative repair, which comprises the following components in parts by weight:
0.5g of recombinant III type humanized collagen, 0.5g of mussel mucin, 0.1g of fibroin peptide, 400 g of polyethylene glycol, and 97.9g of purified water.
The preparation method of the protein liquid dressing for laser and photon postoperative repair comprises the following steps:
1g of polyethylene glycol 400, 97.9g of purified water, 0.5g of recombinant type III humanized collagen, 0.5g of mussel mucin and 0.1g of fibroin peptide are weighed according to the proportion, and then 1g of polyethylene glycol 400 and 97.9g of purified water are mixed and dissolved at room temperature to prepare a solution. Then mixing 0.5g of recombinant III-type humanized collagen, 0.5g of mussel mucin and 0.1g of fibroin peptide with the above solution, uniformly stirring, and sterilizing by cobalt 60 irradiation to obtain the protein liquid dressing for laser and photon postoperative repair. Wherein, in the stirring process, a homogenizing stirrer is used for fully and uniformly mixing the solution, and the solution is subjected to vacuum defoaming.
Example 3
A protein liquid dressing for laser and photon postoperative repair, which comprises the following components in parts by weight:
1.5g of recombinant III type humanized collagen, 1.5g of mussel mucin, 1g of fibroin peptide, 400 g of polyethylene glycol and 91g of purified water.
The preparation method of the protein liquid dressing for laser and photon postoperative repair comprises the following steps:
5g of polyethylene glycol 400, 91g of purified water, 1.5g of recombinant type III humanized collagen, 1.5g of mussel mucin and 1g of fibroin peptide are weighed according to the proportion, and then 5g of polyethylene glycol 400 and 91g of purified water are mixed and dissolved at room temperature to prepare a solution. Then mixing 1.5g of recombinant III-type humanized collagen, 1.5g of mussel mucin and 1g of fibroin peptide with the above solution, uniformly stirring, and carrying out cobalt 60 irradiation sterilization to obtain the protein liquid dressing for laser and photon postoperative repair. Wherein, in the stirring process, a homogenizing stirrer is used for fully and uniformly mixing the solution, and the solution is subjected to vacuum defoaming.
Example 4
A protein liquid dressing for laser and photon postoperative repair, which comprises the following components in parts by weight:
1g of recombinant III type humanized collagen, 0.5g of mussel mucin, 1g of fibroin peptide, 400.5 g of polyethylene glycol and 95g of purified water.
The preparation method of the protein liquid dressing for laser and photon postoperative repair comprises the following steps:
weighing 2.5g of polyethylene glycol 400, 95g of purified water, 1g of recombinant type III humanized collagen, 0.5g of mussel mucin and 1g of fibroin peptide according to a proportion, and then mixing and dissolving 2.5g of polyethylene glycol 400 and 95g of purified water at room temperature to prepare a solution. Then mixing 1g of recombinant III-type humanized collagen, 0.5g of mussel mucin and 1g of fibroin peptide with the above solution, uniformly stirring, and sterilizing by cobalt 60 irradiation to obtain the protein liquid dressing for laser and photon postoperative repair. Wherein, in the stirring process, a homogenizing stirrer is used for fully and uniformly mixing the solution, and the solution is subjected to vacuum defoaming.
Comparative example 1
The protein liquid dressing for laser and photon postoperative repair of the comparative example comprises the following components in parts by weight:
0.2g of recombinant III type humanized collagen, 1g of mussel mucin, 0.05g of fibroin peptide, 400 g of polyethylene glycol 2g and 96.75g of purified water.
The preparation method of the protein liquid dressing for laser and photon postoperative repair of the comparative example is the same as that of example 1 except for the different component contents.
Comparative example 2
The protein liquid dressing for laser and photon postoperative repair of the comparative example comprises the following components in parts by weight:
2g of recombinant III type humanized collagen, 1g of mussel mucin, 1.5g of fibroin peptide, 400 g of polyethylene glycol, and 93.5g of purified water.
The preparation method of the protein liquid dressing for laser and photon postoperative repair of the comparative example is the same as that of example 1 except for the different component contents.
Comparative example 3
The protein liquid dressing for laser and photon postoperative repair of the comparative example comprises the following components in parts by weight:
1g of recombinant III type humanized collagen, 0.2g of mussel mucin, 0.5g of fibroin peptide, 400 g of polyethylene glycol 2g and 96.3g of purified water.
The preparation method of the protein liquid dressing for laser and photon postoperative repair of the comparative example is the same as that of example 1 except for the different component contents.
Comparative example 4
The protein liquid dressing for laser and photon postoperative repair of the comparative example comprises the following components in parts by weight:
1g of recombinant III type humanized collagen, 2g of mussel mucin, 0.5g of fibroin peptide, 400 g of polyethylene glycol, and 94.5g of purified water.
The preparation method of the protein liquid dressing for laser and photon postoperative repair of the comparative example is the same as that of example 1 except for the different component contents.
Dressing use effect contrast
Cell viability and volunteer use feedback tests were performed on the prepared protein liquid dressing for laser, photon post-operative repair to evaluate the safety and effectiveness of the product.
(1) Volunteers evaluate the effectiveness of a product using a feedback test
80 tested volunteers with the ages of 18-60 are selected, the volunteers meet the volunteer selection standard of the tested subjects, the tested subjects are divided into 8 groups on average randomly, 10 persons in each group, and the number of the laser and photon operations in the 10 persons is half. After laser and photon operations are finished, the protein liquid dressings for laser and photon operation repair prepared in examples 1-4 and comparative examples 1-4 are used as test products and are respectively applied to 10 groups of subjects. The using method comprises the following steps: the liquid dressing is smeared on the laser and photon postoperative parts 1 time each day in the morning and evening, and is tried for 14 days. The treatment effect standard is as follows:
the effect is obvious: no scar or pigmentation, and can relieve burning sensation, pain sensation and itching sensation;
the method is effective: the scar and pigmentation are improved, and the burning sensation, the pain sensation and the itching sensation can be relieved;
invalidation: the scar and pigmentation are not obviously improved, and the burning sensation, the pain sensation and the itching sensation cannot be relieved.
The feedback of the subjects was counted after 14 days and the results are shown in table 1.
Table 1: results after 14 days of subject use
In the embodiments 1-4, under the synergistic effect of the recombinant III type humanized collagen, mussel mucin, fibroin peptide, polyethylene glycol and purified water, on one hand, collagen can be directly supplemented to the wound, the wound healing after laser and photon operation is promoted, the formation of scars and pigmentation is avoided, the effects of relieving itching, pain and swelling on the wound are achieved, and the user experience of the product is greatly improved; on the other hand, by promoting collagen synthesis, activating cells, accelerating wound healing, avoiding scar and pigmentation formation, and simultaneously playing a role in postoperative water supplementing. In comparison with examples 1-4, the amount of the recombinant type III humanized collagen and fibroin peptide is too low, which is not beneficial to improving scar and pigmentation of wounds, and can not cooperate with other components, so that the product effect is remarkably reduced; in comparison with examples 1-4, the amounts of recombinant type III humanized collagen and fibroin peptide were too high to greatly improve the effect of the product; in comparison with examples 1-4, comparative examples 3-4 have too low a mussel mucin ratio, are unfavorable for relieving burning, pain and itching after operation, cannot cooperate with other components, and remarkably reduce the effect of the product. The mussel mucin has too high proportion and cannot greatly improve the effect.
(2) Cell viability test, evaluating the safety of the product.
Referring to the experimental method of MTT in vitro cytotoxicity in ISO 10993.5:2016, the protein liquid dressing for laser and photon post-operation repair of preparation examples 1-4 was subjected to in vitro cytotoxicity evaluation, the morphology of the cell survival state was photographed by using an optical microscope, and the cell survival rate was counted according to the OD value.
Analysis of results:
referring to fig. 1, fig. 1 is a morphological image of the cultured cells of example 1, and it can be seen that the growth conditions of the cells are very normal. Referring to fig. 2, the results of the positive control (10% dmso) and the negative control (the culture medium without any treatment) show the feasibility of this in vitro cytotoxicity experiment, and the cell viability results of examples 1-4 demonstrate that the protein liquid dressing for laser and photon postoperative repair developed by the present invention has good cell safety, even can greatly promote proliferation and differentiation of cells, and is very beneficial to wound healing.
Compared with the prior art, the protein liquid dressing for laser and photon postoperative repair can not promote wound healing and reduce wound scars and pigmentation under the synergistic effect of recombinant III-type humanized collagen, mussel mucin, fibroin peptide, polyethylene glycol and purified water; the burn, pain and itching after laser and photon operations can be reduced simultaneously, and the water supplementing device is used for supplementing water after laser and photon operations. The protein liquid dressing for laser and photon postoperative repair can be used for repairing a wound surface after laser and photon operation, and the product is in a liquid form, so that the protein liquid dressing greatly meets the requirements of consumers. According to the invention, through irradiation sterilization, no preservative is added, and the safety is greatly improved.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that modifications and improvements can be made by those skilled in the art without departing from the spirit of the invention, and the invention is intended to encompass such modifications and improvements.
Claims (10)
1. A protein liquid dressing for laser and photon postoperative repair, which is characterized in that: comprises the following components in parts by weight:
recombinant type III humanized collagen: 0.5wt% to 1.5wt%;
mussel mucin: 0.5wt% to 1.5wt%;
fibroin peptide: 0.1wt% to 1.0wt%;
polyethylene glycol: 1.0wt% to 5.0wt%;
purified water: 91.0wt% to 97.9wt%.
2. The proteinaceous liquid dressing for laser, photon post-operative repair of claim 1, wherein: the content of the recombinant type III humanized collagen is 1 weight percent.
3. The proteinaceous liquid dressing for laser, photon post-operative repair of claim 1, wherein: the content of mussel mucin is 1wt%.
4. The proteinaceous liquid dressing for laser, photon post-operative repair of claim 1, wherein: the content of the fibroin peptide is 0.5wt%.
5. The proteinaceous liquid dressing for laser, photon post-operative repair of claim 1, wherein: the content of the polyethylene glycol is 2wt%.
6. The proteinaceous liquid dressing for laser, photon post-operative repair of claim 1 or 5, wherein: the polyethylene glycol is polyethylene glycol 400.
7. A method for preparing a protein liquid dressing for laser and photon postoperative repair according to any one of claims 1-6, wherein: the method comprises the following steps:
weighing the components according to the proportion, and then mixing and dissolving polyethylene glycol and water at room temperature to prepare a solution; mixing the recombinant III type humanized collagen, mussel mucin and fibroin peptide with the solution, uniformly stirring, and carrying out irradiation sterilization to obtain any protein liquid dressing for laser and photon postoperative repair.
8. The method for preparing a protein liquid dressing for laser and photon postoperative repair according to claim 7, wherein the method comprises the following steps: the irradiation sterilization is cobalt 60 irradiation sterilization.
9. The method for preparing a protein liquid dressing for laser and photon postoperative repair according to claim 7, wherein the method comprises the following steps: the solution was vacuum defoamed during stirring.
10. Use of a proteinaceous liquid dressing for laser, photon post-operative repair according to any one of claims 1-6, wherein: the method is used for laser and photon postoperative repair.
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