CN110663649A - Fly maggot breeding method - Google Patents
Fly maggot breeding method Download PDFInfo
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- CN110663649A CN110663649A CN201911016239.7A CN201911016239A CN110663649A CN 110663649 A CN110663649 A CN 110663649A CN 201911016239 A CN201911016239 A CN 201911016239A CN 110663649 A CN110663649 A CN 110663649A
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- 238000009395 breeding Methods 0.000 title claims abstract description 17
- 239000002910 solid waste Substances 0.000 claims abstract description 22
- 241000863432 Shewanella putrefaciens Species 0.000 claims abstract description 20
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 14
- 230000001488 breeding effect Effects 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 13
- 241000894006 Bacteria Species 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 230000001580 bacterial effect Effects 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 4
- 230000007480 spreading Effects 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 241000257159 Musca domestica Species 0.000 description 19
- 230000003385 bacteriostatic effect Effects 0.000 description 10
- 241000863430 Shewanella Species 0.000 description 7
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 238000011160 research Methods 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000004570 mortar (masonry) Substances 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 241000257226 Muscidae Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007124 immune defense Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000015788 innate immune response Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Food Science & Technology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Birds (AREA)
- Insects & Arthropods (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a method for breeding fly maggots, which is characterized in that kitchen organic solid waste added with Shewanella putrefaciens is used for breeding fly maggots, the fly maggots can be induced to generate a specific antibacterial function on the Shewanella putrefaciens, the growth of the fly maggots can be promoted, and the yield of the fly maggots is improved.
Description
Technical Field
The invention relates to a method for breeding fly maggots.
Background
Housefly (Musca domestica L) larvae (maggots) live in the environment where they live across the bacteria without being infected by pathogenic microorganisms therein, and it is presumed that houseflies form a unique immune defense mechanism during the long-term evolution. Researches show that the fly maggots are rich in antibacterial peptides and become an important component of the innate immune response of a host by exerting broad-spectrum antibacterial activity on pathogenic microorganisms. At present, researches on housefly antibacterial peptides mainly focus on separation and purification, activity detection and application of the housefly non-specific antibacterial peptides in animal husbandry, and few researches on inducing housefly larvae to generate specific antibacterial functions by adding certain pathogenic bacteria are carried out.
Disclosure of Invention
The invention aims to provide a method for breeding fly maggots, which can induce the fly maggots to generate specific antibacterial function to Shewanella putrefaciens, promote the growth of the fly maggots and improve the yield of the fly maggots.
In order to realize the purpose, the invention provides a method for breeding fly maggots, which feeds the fly maggots by kitchen organic solid waste added with Shewanella putrefactive bacteria and induces the fly maggots to generate specific antibacterial function to the Shewanella putrefactive bacteria.
Preferably, the method for breeding the fly maggots comprises the following steps:
1) uniformly spreading maggot larvae on the surface of the kitchen organic solid waste for raising maggots; adding a bacterium solution containing Shewanella putrefaciens into the kitchen organic solid waste;
2) collecting mature fly maggots into a clean container and placing for a certain time to fully discharge the food in the fly maggots to obtain fresh fly maggots.
Preferably, in the step 1), the feeding amount of the kitchen organic solid waste is 65-85 kg/m2(ii) a Adding 100ml of bacterial liquid into each kilogram of kitchen organic solid waste, and uniformly stirring; the content of Shewanella putrefaciens in the bacterial liquid is 9 × 109cfu/mL。
Preferably, in the step 1), the breeding density of the fly maggots is as follows: 1-10 g of fly maggot larvae are thrown into each kilogram of kitchen organic solid waste.
Preferably, in the step 1), the fly maggot breeding environment is as follows: the temperature is controlled to be 25-26 ℃, the air humidity is 65%, the sunlight irradiation is avoided, and the ventilation is kept.
Preferably, in the step 1), the breeding time of the fly maggots is 4-5 days.
Preferably, in the step 2), the mature fly maggots are collected into a clean container and are placed for 4-6 hours.
The invention has the advantages and beneficial effects that: the breeding method of the fly maggots is provided, the Shewanella putrefaciens is applied, the specific antibacterial function of the fly maggots on the Shewanella putrefaciens can be induced, the growth of the fly maggots can be promoted, and the yield of the fly maggots is improved.
Drawings
FIG. 1 is a photograph showing the bacteriostatic effect of homogenate of functional fly maggots and general fly maggots against Shewanella putrefaciens.
Detailed Description
The following description of the embodiments of the present invention will be made with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
The invention provides a method for breeding fly maggots, which feeds the fly maggots by kitchen organic solid waste added with Shewanella putrefaciens and induces the fly maggots to generate a specific antibacterial function to the Shewanella putrefaciens, and comprises the following steps:
1) uniformly spreading and drying the fly larvae on the surface of the kitchen organic solid waste, and culturing the fly larvae for 4-5 days;
the feeding amount of the kitchen organic solid waste is 65-85 kg/m2(ii) a Adding a bacterium solution containing Shewanella putrefaciens into the kitchen organic solid waste, and adding 100ml of the bacterium solution into each kilogram of the kitchen organic solid waste and uniformly stirring; the content of Shewanella putrefaciens in the bacterial liquid is 9 × 109cfu/mL；
And (3) breeding density of fly maggots: 1-10 g of fly maggot larvae are thrown into each kilogram of kitchen organic solid waste;
the fly maggot breeding environment: the temperature is controlled to be 25-26 ℃, the air humidity is 65%, the sunlight is prevented from irradiating, and ventilation is kept;
2) and collecting mature fly larvae into a clean container, and placing for 4-6 hours to fully discharge the food in the fly larvae to obtain the fresh fly larvae.
The comparative tests were as follows:
1 fly maggot culture
2000g of kitchen organic solid waste is added into two plastic trays respectively, and bacterial liquid (the content of Shewanella putrefaciens in the bacterial liquid is 9 multiplied by 10)9cfu/mL) 200mL, and stirring evenly; in addition200ml of normal saline is added into one plate and evenly stirred; then 20g of newly hatched housefly larvae (1.2 mg/fly larvae) are respectively put into the two plates, the room temperature of an air conditioner is kept at 25-26 ℃, the air humidity is 65%, and the two plates are cultivated for 4 days to respectively obtain two kinds of mature housefly larvae, namely functional housefly larvae induced by the separating bacteria and common housefly larvae which are not induced.
The two groups of housefly larvae are cultured for 4 days to respectively obtain 525.0g of mature Shewanella putrefaction induced functional housefly maggots (the average weight is 31.5 g/one) and 406.7g of uninduced common housefly maggots (the average weight is 24.4 g/one), and the weight of the former is increased by 29.1 percent compared with that of the latter.
2 bacteriostatic test
Two mortars are used for fully homogenizing functional fly maggots and common fly maggots (1 g each), 10ml of normal saline is added into each mortar, and two kinds of homogenate are obtained after uniform mixing. Uniformly coating the separated bacterial liquid on a TSA (TSA) plate, drilling two holes (the hole radius is 5 mm) at a certain distance on the plate, adding 0.1ml of homogenate into each hole, culturing in an incubator at 28 ℃ for 24h, and observing the antibacterial effect.
The bacteriostatic effect of the homogenate of functional fly maggots and common fly maggots on Shewanella putrefaciens is shown in figure 1. FIG. 1 shows that the radius of inhibition zone of functional fly maggot homogenate and ordinary fly maggot homogenate on a TSA plate against Shewanella putrefaciens is 16mm and 7mm, respectively, the former being 2.3 times that of the latter.
3 comparison conclusion
The result of the housefly maggot culture test shows that the average weight of mature housefly maggots (functional housefly maggots) induced and cultured by the kitchen organic solid waste added with a proper amount of Shewanella putrefaction bacterium liquid is 31.5 g/piece, the average weight of mature housefly maggots (common housefly maggots) cultured by pure kitchen organic solid waste is 24.4 g/piece, and the weight of the mature housefly maggots is increased by 29.1 percent compared with that of the mature housefly maggots. The Shewanella putrefaciens can not only cause the disease of the fly maggots, but also promote the growth of the fly maggots.
The results of bacteriostatic tests show that on a TSA plate, radius of a bacteriostatic circle of functional fly maggots induced by the spoilage Shewanella bacteria and radius of a bacteriostatic circle of common non-induced fly maggots on the bacteria are respectively 16mm and 7mm, wherein the radius of the bacteriostatic circle of the functional fly maggots and the radius of the bacteriostatic circle of the common fly maggots are 2.3 times that of the latter, which shows that the functional fly maggots and the homogenate of the common fly maggots have obvious bacteriostatic effect on the spoilage Shewanella bacteria, which shows that broad-spectrum antibacterial active substances (mainly comprising antibacterial peptides) exist in the fly maggots, and the bacteriostatic effect of the functional fly maggots is obviously higher than that of the common fly maggots, and simultaneously shows that the spoilage Shewanella.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (7)
1. The method for breeding the fly maggots is characterized in that the kitchen organic solid waste added with the Shewanella putrefaciens is used for breeding the fly maggots, and the fly maggots are induced to generate a specific antibacterial function on the Shewanella putrefaciens.
2. The fly maggot culturing method according to claim 1, comprising the steps of:
1) uniformly spreading maggot larvae on the surface of the kitchen organic solid waste for raising maggots; adding a bacterium solution containing Shewanella putrefaciens into the kitchen organic solid waste;
2) collecting mature fly maggots into a clean container and placing for a certain time to fully discharge the food in the fly maggots to obtain fresh fly maggots.
3. The fly maggot culturing method according to claim 2, wherein the feeding amount of the kitchen organic solid waste in the step 1) is 65-85 kg/m2(ii) a Adding 100ml of bacterial liquid into each kilogram of kitchen organic solid waste, and uniformly stirring; the content of Shewanella putrefaciens in the bacterial liquid is 9 × 109cfu/mL。
4. The method for culturing maggots according to claim 3, wherein in the step 1), the density of the maggots cultured is: 1-10 g of fly maggot larvae are thrown into each kilogram of kitchen organic solid waste.
5. The method for culturing maggots according to claim 4, wherein in the step 1), the environment for culturing the maggots is as follows: the temperature is controlled to be 25-26 ℃, the air humidity is 65%, the sunlight irradiation is avoided, and the ventilation is kept.
6. The method for culturing maggots according to claim 5, wherein the period of time for culturing the maggots in step 1) is 4 to 5 days.
7. The method for culturing maggots according to claim 6, wherein in the step 2), the mature maggots are collected in a clean container and placed for 4-6 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911016239.7A CN110663649A (en) | 2019-10-24 | 2019-10-24 | Fly maggot breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911016239.7A CN110663649A (en) | 2019-10-24 | 2019-10-24 | Fly maggot breeding method |
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| CN110663649A true CN110663649A (en) | 2020-01-10 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101333254A (en) * | 2007-06-29 | 2008-12-31 | 上海市上海中学 | Method for preparing drosophila melanogaster antimicrobial peptide |
| CN103947613A (en) * | 2014-05-09 | 2014-07-30 | 江苏洁净环境科技有限公司 | Cultivation method for fly maggots |
| WO2015015033A1 (en) * | 2013-07-31 | 2015-02-05 | Universidad De Malaga | Bioencapsulation of the probiotic shewanella putrefaciens pdp11 in living food and uses |
| CN108902447A (en) * | 2018-06-22 | 2018-11-30 | 苏州净瑞环保科技有限公司 | Disease prevention type fly maggot feeding feed, preparation method and prophylactic fly-maggot feed |
| CN110226568A (en) * | 2019-07-22 | 2019-09-13 | 中国科学院水生生物研究所 | A kind of application in terms of regulating and controlling aquatic animal intestinal health of method and fly maggot improving fly maggot antibacterial peptide fungistatic effect |
-
2019
- 2019-10-24 CN CN201911016239.7A patent/CN110663649A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101333254A (en) * | 2007-06-29 | 2008-12-31 | 上海市上海中学 | Method for preparing drosophila melanogaster antimicrobial peptide |
| WO2015015033A1 (en) * | 2013-07-31 | 2015-02-05 | Universidad De Malaga | Bioencapsulation of the probiotic shewanella putrefaciens pdp11 in living food and uses |
| CN103947613A (en) * | 2014-05-09 | 2014-07-30 | 江苏洁净环境科技有限公司 | Cultivation method for fly maggots |
| CN108902447A (en) * | 2018-06-22 | 2018-11-30 | 苏州净瑞环保科技有限公司 | Disease prevention type fly maggot feeding feed, preparation method and prophylactic fly-maggot feed |
| CN110226568A (en) * | 2019-07-22 | 2019-09-13 | 中国科学院水生生物研究所 | A kind of application in terms of regulating and controlling aquatic animal intestinal health of method and fly maggot improving fly maggot antibacterial peptide fungistatic effect |
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Application publication date: 20200110 |