CN110607223A

CN110607223A - Cattle and sheep embryo incubator

Info

Publication number: CN110607223A
Application number: CN201910981880.8A
Authority: CN
Inventors: 陈恺宇; 梁晶婕
Original assignee: Zhejiang University ZJU
Current assignee: Zhejiang University ZJU
Priority date: 2019-10-16
Filing date: 2019-10-16
Publication date: 2019-12-24
Anticipated expiration: 2039-10-16
Also published as: CN110607223B; JP6737989B1; JP2021061818A

Abstract

The invention discloses a cattle and sheep embryo incubator, which comprises a box body and a bearing plate, wherein a plurality of communicated compartments are arranged on the box body, culture dishes are arranged in the compartments, a first movable cavity is arranged at the top of each compartment, and a first baffle is arranged in each first movable cavity; a first connecting plate corresponding to the bearing plate is arranged on the side wall of the box body, a first through cavity matched with the separation bin is arranged on the bearing plate, an oxygen generating device and a carbon dioxide generating device are arranged on the bearing plate, a second baffle plate is inserted into the first through cavity, a first connecting block is arranged on the bearing plate, and a connecting groove matched with the first connecting block is arranged on the side wall of the box body; through the mode of connecting the adapting plate, adjust the environment of first logical intracavity earlier, open the compartment again, avoid the external air directly to enter into the box in cause the influence to the balance in the box, prevent to cause the influence to other culture dishes when taking out a culture dish alone, promote embryo survival rate.

Description

Cattle and sheep embryo incubator

Technical Field

The invention belongs to the technical field of incubators, and particularly relates to a cattle and sheep embryo incubator.

Background

At present, the cattle and sheep artificial fertilization technology in China is very popular, plays an important role in improving the cattle and sheep varieties in China, continuously improves the improved variety coverage rate, and plays a promoting role in the national economic development of China.

Artificial insemination includes the external culture insemination, be about to the embryo of cultivateing in the incubator after sperm and ovum are taken out, in order to promote ox sheep embryo survival rate, an incubator for cultivateing the embryo generally is encapsulated situation, there is great difference in environment and the external environment in the incubator, there are a plurality of culture dishes usually in an incubator, when taking out individual culture dish, the external air directly enters into the incubator, cause the influence to the embryo in other culture dishes, destroy the environment in the incubator, the survival rate of embryo has been reduced.

Disclosure of Invention

The invention provides a cattle and sheep embryo incubator in order to overcome the defects of the prior art.

In order to achieve the purpose, the invention adopts the following technical scheme: a cattle and sheep embryo incubator comprises a box body and a bearing plate, wherein a plurality of communicated separation bins are arranged on the box body, culture dishes are arranged in the separation bins, a first movable cavity is arranged at the top of each separation bin, and a first baffle is arranged in each first movable cavity; a first connecting plate corresponding to the bearing plate is arranged on the side wall of the box body, a first through cavity matched with the separation bin is arranged on the bearing plate, an oxygen generating device and a carbon dioxide generating device are arranged on the bearing plate, a second baffle plate is inserted into the first through cavity, a first connecting block is arranged on the bearing plate, and a connecting groove matched with the first connecting block is arranged on the side wall of the box body; the embryo is placed in a culture dish for culture, and airflow flows among the compartments, so that all the compartments are in a consistent environment, and the embryo growth is facilitated; when a culture dish in one of the compartments needs to be taken out, the bearing plate is inserted into the connecting groove, the connecting groove is matched with the first connecting block, the bearing plate is fixed on one side of the compartment, the first through cavity forms a closed environment under the condition that the first baffle plate and the second baffle plate are matched with each other, the oxygen generating device and the carbon dioxide generating device work to inject airflow into the first through cavity, the gas environment in the first through cavity is adjusted to be the same as that in the box body, then the first baffle plate is opened, the culture dish is pushed into the first through cavity, after the culture dish completely enters the first through cavity, the compartment is closed by the first baffle plate, and the culture dish is taken out from the first through cavity by opening the second baffle plate; by connecting the bearing plates, the environment in the first through cavity is adjusted, and then the compartment is opened, so that the influence of external air directly entering the box body on the balance in the box body is avoided, the influence on other culture dishes when one culture dish is taken out independently is prevented, and the survival rate of embryos is improved; the mode of adjusting the internal environment of the first through cavity is used for replacing the mode of adjusting the internal environment of the whole box body, so that the difficulty of adjusting the internal environment of the box body is reduced, the environment adjusting speed is increased by using the smaller volume of the first through cavity, and the energy consumption required by adjusting the environment is reduced; under the mutual matching of the first connecting block and the connecting grooves, the bearing plate is moved to the corresponding compartment position as required, so that the culture dish in any compartment can be taken out, and the use convenience of the bearing plate is improved; the bearing plate is arranged on the first connecting plate when not in use, so that a storage space is provided for the bearing plate, and the situation that the bearing plate blocks the culture dish is avoided.

A second through cavity is formed in the side wall of the compartment, a first movable cavity is formed in the inner wall of the second through cavity, a first push rod penetrates through the second through cavity, a first limiting plate matched with the first movable cavity is arranged on the first push rod, and a lengthening rod assembly is arranged on the first push rod; after the bearing plate is fixed on the side wall of the compartment, a first push rod is pushed into the box body and is in contact with the culture dish, the culture dish is pushed out of the compartment under the action of the first push rod, so that the culture dish enters the first through cavity, and the condition that the environment in the box body is damaged when the culture dish is taken out of the box body due to the fact that outside air enters the box body is avoided; under the extension bar subassembly effect, provide longer arm of force for first push rod to push whole first push rod and release the culture dish in the compartment, make the culture dish whole enter into first logical intracavity, provide through first logical chamber for the culture dish and hold the station, it is effectual isolated mutually with box internal environment and external environment.

The first push rod is provided with a second connecting plate, the extension rod assembly comprises a first connecting rod rotatably connected onto the second connecting plate, a second connecting rod arranged on the first connecting rod and a third connecting plate arranged on the second connecting rod, the first connecting rod is provided with a first movable groove, one end of the second connecting rod is rotatably connected onto one end of the first movable groove, the other end of the second connecting rod is movably connected onto the third connecting plate, and the side wall of the box body is provided with a first groove matched with the first connecting rod; when the culture dish is pushed into the first through cavity, the third connecting plate is pulled outwards, the third connecting plate drives one end of the second connecting rod to move outwards, the second connecting rod rotates around the connecting point, the first connecting rod is driven to rotate around the connecting point when the second connecting rod rotates, and after the first connecting rod and the second connecting rod rotate to be in a straight line state, the second connecting rod is pushed towards the inside of the box body, so that the second connecting rod pushes the first push rod to move towards the inside of the box body, and the culture dish is pushed out of the compartment so as to be taken out of the box body; when the culture dish is in the compartment, the first connecting rods are pulled towards the two sides, the third connecting plates are pushed towards the direction of the box body after the first connecting rods and the second connecting rods form a quadrilateral structure, the second connecting rods enter the first movable groove, the first connecting rods enter the first groove, the first connecting rods are stored through the first groove, and potential safety hazards caused by the fact that the first connecting rods and the second connecting rods protrude are avoided.

A second movable groove is formed in the side wall of the first movable cavity, a push plate matched with the second movable groove is arranged on the first baffle, a guide frame is arranged on the bearing plate, a second connecting block matched with the push plate is arranged in the guide frame, a first sliding block is arranged on the side wall of the second connecting block, and a first sliding groove matched with the first sliding block is formed in the inner wall of the guide frame; when the bearing plate is fixed on the side wall of the compartment, the second connecting block is matched with the push plate, the push plate is moved in a mode of moving the second connecting block, so that the push plate drives the first baffle plate to move upwards, and the compartment is opened, so that the culture dish is pushed out of the compartment; set up down at the second connecting block, for the push pedal provides the impetus, the person of facilitating the use directly promotes first baffle and removes, makes the use of incubator more convenient.

A third through cavity matched with the push plate is arranged on the second connecting block, a second sliding groove is formed in the side wall of the push plate, and a second sliding block matched with the second sliding groove is arranged on the inner wall of the third through cavity; a first return spring is arranged on the first sliding block; when the bearing plate is fixed on the side wall of the compartment, the second connecting block moves towards the push plate, and the second sliding block is embedded into the second sliding groove, so that the second connecting block is matched with the push plate, and the push plate is driven to move together when the second connecting block moves; under the mutually supporting of second slider and second spout, make the second connecting block change and form the cooperation with the push pedal, reduce and accept the board installation degree of difficulty.

A third movable groove is formed in the side wall of the second connecting block, a limiting spring is arranged in the third movable groove, a limiting block is arranged at one end of the limiting spring, and a limiting groove matched with the limiting block is formed in the inner wall of the guide frame; a fourth through cavity is formed in one end of the limiting groove, a second movable cavity is formed in the inner wall of the fourth through cavity, a second push rod penetrates through the fourth through cavity, a second limiting plate matched with the second movable cavity is arranged on the second push rod, and a second reset spring is arranged on the second limiting plate; after the bearing plate and the box body are installed, the second connecting block is pushed to move upwards, the second connecting block moves to the top of the guide frame, the limiting block moves to one side of the limiting groove, the limiting block enters the limiting groove under the action of the limiting spring to fix the second connecting block, and the first baffle is continuously in an open state so that the culture dish is pushed out of the compartment under the action of the first push rod; after the culture dish enters into first logical intracavity completely, promote the second push rod toward the spacing inslot motion, make the second push rod promote the stopper and deviate from the spacing inslot, promote the second connecting block and down move under the effect of first reset spring, make first baffle seal the compartment fast to do the adjustment to the environment of first logical intracavity alone, avoid the external air to enter into the box and cause the influence to the box environment.

The carbon dioxide generating device comprises a supporting table, a reaction box arranged at the top of the supporting table and a reagent bottle arranged on the reaction box, wherein a reaction cavity is arranged in the reaction box, a connecting ring is arranged at the top of the reaction box, the reagent bottle is connected to the connecting ring through threads, a valve assembly is arranged on the reagent bottle, and a first air cylinder used for controlling the valve assembly to be opened is arranged in the reaction cavity; after the bearing plate is fixed on the side wall of the box body, the reagent in the reagent bottle enters the reaction box, carbon dioxide is generated in the reaction box and then is introduced into the first through cavity, and the environment in the first through cavity is adjusted; the reagent bottle is sealed under the action of the valve component, so that the reagent is prevented from being poured out of the reagent bottle when the reagent bottle is installed, and the waste of the reagent is reduced; controlling the discharge amount of the reagent bottle under the action of a first air cylinder so as to introduce a specific amount of carbon dioxide into the first through cavity and adjust the environment in the first through cavity; through the threaded connection of reagent bottle and go-between, can be convenient pull down the reagent bottle from the reaction box, supply reagent to the realization leads to the adjustment of intracavity environment to first.

A cavity is formed in the supporting table, a waste liquid box penetrates through the cavity, a first through groove matched with the reaction cavity is formed in the top of the supporting table, a partition plate is rotatably connected to the inner wall of the first through groove, a first mounting plate is arranged at the top of the cavity, a second air cylinder used for driving the partition plate to turn over is arranged on the first mounting plate, and a second groove matched with the second air cylinder is formed in the waste liquid box; after the bearing plate is fixed on the side wall of the box body, reagents in the reagent bottles enter the reaction box to react, the partition plate is in a horizontal state, and the first through groove is sealed, so that the reagents normally react in the reaction cavity to generate gas; after the reaction is finished, the second air cylinder drives the partition plate to turn over for a specified angle, so that the partition plate is in an inclined state, the reaction liquid on the partition plate flows down along the surface of the partition plate, the reaction liquid is poured into the waste liquid box, and the reacted reagent is collected so as to be conveniently treated; the waste liquid box is prevented from colliding with the second cylinder during installation under the second groove, the waste liquid box and the supporting table are stably matched, the waste liquid is prevented from influencing the reaction, and the reaction is normally carried out.

A fourth movable groove is formed in the bottom of the partition plate, a fourth connecting plate is rotatably connected in the fourth movable groove, one side of the fourth connecting plate is in contact with the inner wall of the fourth movable groove, a third connecting rod is arranged on the piston rod of the second air cylinder, and the third connecting rod is movably connected to the fourth connecting plate; a fixing groove is formed in the third groove, a second through groove is formed in the top of the cavity, and a fixing plate matched with the fixing groove penetrates through the second through groove; the fourth connecting plate is in contact with the inner wall of the fourth movable groove, and has a supporting effect on the fourth connecting plate, so that the fourth connecting plate cannot rotate continuously after rotating to a vertical state, and a good supporting effect is provided for the partition plate; after the reaction of the reagent in the reaction cavity is finished, the second cylinder drives the piston rod to contract, the third connecting rod drives the fourth connecting plate to move back, the fourth connecting plate rotates around the connecting point, the fourth connecting plate rotates to an inclined state, one end of the partition plate is pulled to move downwards, the partition plate is in the inclined state, so that reaction liquid in the reaction cavity flows down into the waste liquid box along the partition plate, waste liquid is collected, and the phenomenon that the culture dish is influenced by the pouring of the waste liquid is avoided; when the second cylinder pushes the piston rod to move forwards, the third connecting rod pushes the fourth connecting plate to rotate to a vertical state, the side wall of the fourth connecting plate is in contact with the inner wall of the fourth movable groove to provide supporting force for the fourth connecting plate, so that the fourth connecting plate cannot rotate continuously, the partition plate is in a horizontal state to seal the first through groove, and reagents can react in the reaction cavity; after the waste liquid box is placed into the cavity, the fixing groove is positioned below the fixing plate, the fixing plate is pushed into the fixing groove, so that the fixing plate is matched with the fixing groove, the waste liquid box is fixed, and the waste liquid box is prevented from being moved out from the space; the fixed plate seals the second groove after inserting in the fixed slot, prevents that the waste liquid in the waste liquid box from pouring out from the second groove, avoids chemical agent to reveal and causes the pollution to the environment.

The valve component comprises a circular ring arranged on the inner wall of the reagent bottle mouth and a third baffle plate arranged on the circular ring, a third sliding block is arranged on the side wall of the third baffle plate, a third sliding groove matched with the third sliding block is arranged on the inner wall of the reagent bottle mouth, and a sealing spring is arranged on the third sliding block; the third baffle plate is matched with the circular ring, the circular ring is sealed under the action of the third baffle plate, the reagent bottle is sealed, and the reagent is prevented from being directly poured out of the reagent bottle; after the bearing plate is fixed on the side wall of the box body, the first cylinder drives the piston rod to extend out, the piston rod of the first cylinder penetrates through the circular ring to be in contact with the third baffle plate, the first cylinder pushes the third baffle plate to move upwards, so that a gap is formed between the third baffle plate and the circular ring, and the third baffle plate and the circular ring actually fall into the reaction cavity from the circular ring hole, so that carbon dioxide is provided for the first cavity to adjust the environment in the first cavity.

The invention has the following advantages: through the mode of connecting the adapting plate, adjust the environment of first logical intracavity earlier, open the compartment again, avoid the external air directly to enter into the box in cause the influence to the balance in the box, prevent to cause the influence to other culture dishes when taking out a culture dish alone, promote embryo survival rate.

Drawings

FIG. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a bottom view of the present invention.

Fig. 3 is a first cross-sectional view of the present invention.

Fig. 4 is an enlarged view of a portion a in fig. 3.

Fig. 5 is an enlarged view of fig. 3 at B.

Fig. 6 is an enlarged view of fig. 3 at C.

Fig. 7 is an enlarged view of fig. 3 at D.

Fig. 8 is an enlarged view of fig. 3 at E.

FIG. 9 is a second cross-sectional view of the present invention.

Fig. 10 is an enlarged view of fig. 9 at F.

Fig. 11 is an enlarged view at G in fig. 9.

Fig. 12 is a third schematic sectional view of the present invention.

Fig. 13 is an enlarged view of fig. 12 at H.

Fig. 14 is a cross-sectional view of the fourth embodiment of the present invention.

Fig. 15 is an enlarged view at I in fig. 14.

Fig. 16 is a cross-sectional view of the fifth embodiment of the present invention.

Fig. 17 is an enlarged view at J in fig. 16.

Fig. 18 is an enlarged view at K in fig. 16.

Detailed Description

As shown in fig. 1-18, a cattle and sheep embryo incubator comprises a box body 1 and a bearing plate 2, wherein a plurality of communicated compartments are arranged on the box body 1, culture dishes 3 are arranged in the compartments, a first movable cavity is arranged at the top of each compartment, and a first baffle 11 is arranged in each first movable cavity; a first connecting plate 12 corresponding to the bearing plate 2 is arranged on the side wall of the box body 1, and a gas regulating device and a first single chip microcomputer are arranged in the box body to control the environment in the box body and provide a good environment for embryo development; the bearing plate 2 is provided with a first through cavity matched with the separation bin, the bearing plate 2 is provided with an oxygen generating device and a carbon dioxide generating device, the bearing plate is provided with a second single chip microcomputer, and the second single chip microcomputer controls the oxygen generating device and the carbon dioxide generating device to work so as to adjust the environment in the first through cavity; a second baffle 21 is inserted into the first through cavity, a first connecting block 210 is arranged on the bearing plate 2, and a connecting groove matched with the first connecting block 210 is arranged on the side wall of the box body 1; the embryo is placed in a culture dish for culture, and airflow flows among the compartments, so that all the compartments are in a consistent environment, and the embryo growth is facilitated; when a culture dish in one of the compartments needs to be taken out, the bearing plate is inserted into the connecting groove, the connecting groove is matched with the first connecting block, the bearing plate is fixed on one side of the compartment, the first through cavity forms a closed environment under the condition that the first baffle plate and the second baffle plate are matched with each other, the oxygen generating device and the carbon dioxide generating device work to inject airflow into the first through cavity, the gas environment in the first through cavity is adjusted to be the same as that in the box body, then the first baffle plate is opened, the culture dish is pushed into the first through cavity, after the culture dish completely enters the first through cavity, the compartment is closed by the first baffle plate, and the culture dish is taken out from the first through cavity by opening the second baffle plate; by connecting the bearing plates, the environment in the first through cavity is adjusted, and then the compartment is opened, so that the influence of external air directly entering the box body on the balance in the box body is avoided, the influence on other culture dishes when one culture dish is taken out independently is prevented, and the survival rate of embryos is improved; the mode of adjusting the internal environment of the first through cavity is used for replacing the mode of adjusting the internal environment of the whole box body, so that the difficulty of adjusting the internal environment of the box body is reduced, the environment adjusting speed is increased by using the smaller volume of the first through cavity, and the energy consumption required by adjusting the environment is reduced; under the mutual matching of the first connecting block and the connecting grooves, the bearing plate is moved to the corresponding compartment position as required, so that the culture dish in any compartment can be taken out, and the use convenience of the bearing plate is improved; the bearing plate is arranged on the first connecting plate when not in use, so that a storage space is provided for the bearing plate, and the situation that the bearing plate blocks the culture dish is avoided.

A second through cavity is formed in the side wall of the compartment, a first movable cavity is formed in the inner wall of the second through cavity, a first push rod 14 penetrates through the second through cavity, a first limiting plate 141 matched with the first movable cavity is arranged on the first push rod 14, and a lengthening rod assembly is arranged on the first push rod 14; after the bearing plate is fixed on the side wall of the compartment, a first push rod is pushed into the box body and is in contact with the culture dish, the culture dish is pushed out of the compartment under the action of the first push rod, so that the culture dish enters the first through cavity, and the condition that the environment in the box body is damaged when the culture dish is taken out of the box body due to the fact that outside air enters the box body is avoided; under the extension bar subassembly effect, provide longer arm of force for first push rod to push whole first push rod and release the culture dish in the compartment, make the culture dish whole enter into first logical intracavity, provide through first logical chamber for the culture dish and hold the station, it is effectual isolated mutually with box internal environment and external environment.

A second connecting plate is arranged on the first push rod 14, the extension rod assembly comprises a first connecting rod 15 rotatably connected onto the second connecting plate, a second connecting rod 16 arranged on the first connecting rod 15 and a third connecting plate 17 arranged on the second connecting rod 16, a first movable groove is arranged on the first connecting rod 15, one end of the second connecting rod 16 is rotatably connected onto one end of the first movable groove, the other end of the second connecting rod 16 is movably connected onto the third connecting plate 17, and a first groove matched with the first connecting rod 15 is arranged on the side wall of the box body 1; when the culture dish is pushed into the first through cavity, the third connecting plate is pulled outwards, the third connecting plate drives one end of the second connecting rod to move outwards, the second connecting rod rotates around the connecting point, the first connecting rod is driven to rotate around the connecting point when the second connecting rod rotates, and after the first connecting rod and the second connecting rod rotate to be in a straight line state, the second connecting rod is pushed towards the inside of the box body, so that the second connecting rod pushes the first push rod to move towards the inside of the box body, and the culture dish is pushed out of the compartment so as to be taken out of the box body; when the culture dish is in the compartment, the first connecting rods are pulled towards the two sides, the third connecting plates are pushed towards the direction of the box body after the first connecting rods and the second connecting rods form a quadrilateral structure, the second connecting rods enter the first movable groove, the first connecting rods enter the first groove, the first connecting rods are stored through the first groove, and potential safety hazards caused by the fact that the first connecting rods and the second connecting rods protrude are avoided.

A second movable groove is formed in the side wall of the first movable cavity, a push plate 111 matched with the second movable groove is arranged on the first baffle plate 11, a guide frame 28 is arranged on the bearing plate 2, a second connecting block 29 matched with the push plate 111 is arranged in the guide frame 28, a first sliding block is arranged on the side wall of the second connecting block, and a first sliding groove matched with the first sliding block is formed in the inner wall of the guide frame 28; when the bearing plate is fixed on the side wall of the compartment, the second connecting block is matched with the push plate, the push plate is moved in a mode of moving the second connecting block, so that the push plate drives the first baffle plate to move upwards, and the compartment is opened, so that the culture dish is pushed out of the compartment; set up down at the second connecting block, for the push pedal provides the impetus, the person of facilitating the use directly promotes first baffle and removes, makes the use of incubator more convenient.

A third through cavity matched with the push plate 111 is arranged on the second connecting block 29, a second sliding groove is arranged on the side wall of the push plate 111, and a second sliding block matched with the second sliding groove is arranged on the inner wall of the third through cavity; a first return spring 293 is arranged on the first sliding block; when the bearing plate is fixed on the side wall of the compartment, the second connecting block moves towards the push plate, and the second sliding block is embedded into the second sliding groove, so that the second connecting block is matched with the push plate, and the push plate is driven to move together when the second connecting block moves; under the mutually supporting of second slider and second spout, make the second connecting block change and form the cooperation with the push pedal, reduce and accept the board installation degree of difficulty.

A third movable groove is formed in the side wall of the second connecting block 29, a limiting spring 291 is arranged in the third movable groove, a limiting block 292 is arranged at one end of the limiting spring 291, and a limiting groove matched with the limiting block 292 is formed in the inner wall of the guide frame 28; a fourth through cavity is formed in one end of the limiting groove, a second movable cavity is formed in the inner wall of the fourth through cavity, a second push rod 281 penetrates through the fourth through cavity, a second limiting plate 2811 matched with the second movable cavity is arranged on the second push rod 281, and a second reset spring 2812 is arranged on the second limiting plate 2811; after the bearing plate and the box body are installed, the second connecting block is pushed to move upwards, the second connecting block moves to the top of the guide frame, the limiting block moves to one side of the limiting groove, the limiting block enters the limiting groove under the action of the limiting spring to fix the second connecting block, and the first baffle is continuously in an open state so that the culture dish is pushed out of the compartment under the action of the first push rod; after the culture dish enters into first logical intracavity completely, promote the second push rod toward the spacing inslot motion, make the second push rod promote the stopper and deviate from the spacing inslot, promote the second connecting block and down move under the effect of first reset spring, make first baffle seal the compartment fast to do the adjustment to the environment of first logical intracavity alone, avoid the external air to enter into the box and cause the influence to the box environment.

The carbon dioxide generating device comprises a supporting table 22, a reaction box 24 arranged at the top of the supporting table 22 and a reagent bottle 25 arranged on the reaction box 24, wherein a reaction cavity is arranged in the reaction box 24, a connecting pipe 241 is arranged on the side wall of the reaction box, the reaction box is communicated with the first through cavity through the connecting pipe, the connecting pipe is arranged at the top of the side wall of the reaction box, so that a reagent is prevented from entering the connecting pipe, and gas generated in the reaction cavity is introduced into the first through cavity through the connecting pipe; the top of the reaction box 24 is provided with a connecting ring 242, the reagent bottle 25 is connected to the connecting ring 242 through threads, the inner wall of the connecting ring is provided with a support ring 243, the support ring limits the lowest position of the reagent bottle, so that the reagent bottle and the connecting ring are better connected, the reagent bottle 25 is provided with a valve assembly, a first air cylinder 245 for controlling the opening of the valve assembly is arranged in the reaction cavity, a second mounting plate 244 is arranged in the reaction cavity, the first air cylinder is arranged on the second mounting plate, and the first air cylinder is arranged under the connecting ring; after the bearing plate is fixed on the side wall of the box body, the reagent in the reagent bottle enters the reaction box, carbon dioxide is generated in the reaction box and then is introduced into the first through cavity, and the environment in the first through cavity is adjusted; the reagent bottle is sealed under the action of the valve component, so that the reagent is prevented from being poured out of the reagent bottle when the reagent bottle is installed, and the waste of the reagent is reduced; controlling the discharge amount of the reagent bottle under the action of a first air cylinder so as to introduce a specific amount of carbon dioxide into the first through cavity and adjust the environment in the first through cavity; through the threaded connection of reagent bottle and go-between, can be convenient pull down the reagent bottle from the reaction box, supply reagent to the realization leads to the adjustment of intracavity environment to first.

A cavity is formed in the supporting table 22, a waste liquid box 27 penetrates through the cavity, a first through groove matched with the reaction cavity is formed in the top of the supporting table 22, a partition plate 221 is rotatably connected to the inner wall of the first through groove, a first mounting plate 224 is arranged at the top of the cavity, a second air cylinder 225 used for driving the partition plate 221 to overturn is arranged on the first mounting plate 224, and a second groove matched with the second air cylinder 225 is formed in the waste liquid box 27; after the bearing plate is fixed on the side wall of the box body, reagents in the reagent bottles enter the reaction box to react, the partition plate is in a horizontal state, and the first through groove is sealed, so that the reagents normally react in the reaction cavity to generate gas; after the reaction is finished, the second air cylinder drives the partition plate to turn over for a specified angle, so that the partition plate is in an inclined state, the reaction liquid on the partition plate flows down along the surface of the partition plate, the reaction liquid is poured into the waste liquid box, and the reacted reagent is collected so as to be conveniently treated; the waste liquid box is prevented from colliding with the second cylinder during installation under the second groove, the waste liquid box and the supporting table are stably matched, the waste liquid is prevented from influencing the reaction, and the reaction is normally carried out.

A fourth movable groove is formed in the bottom of the partition 221, a fourth connecting plate 222 is rotatably connected in the fourth movable groove, one side of the fourth connecting plate 222 is in contact with the inner wall of the fourth movable groove, a piston rod of the second cylinder 225 is provided with a third connecting rod 223, and the third connecting rod 223 is movably connected to the fourth connecting plate 222; a fixing groove is formed in the third groove, a second through groove is formed in the top of the cavity, and a fixing plate 226 matched with the fixing groove penetrates through the second through groove; the fourth connecting plate is in contact with the inner wall of the fourth movable groove, and has a supporting effect on the fourth connecting plate, so that the fourth connecting plate cannot rotate continuously after rotating to a vertical state, and a good supporting effect is provided for the partition plate; after the reaction of the reagent in the reaction cavity is finished, the second cylinder drives the piston rod to contract, the third connecting rod drives the fourth connecting plate to move back, the fourth connecting plate rotates around the connecting point, the fourth connecting plate rotates to an inclined state, one end of the partition plate is pulled to move downwards, the partition plate is in the inclined state, so that reaction liquid in the reaction cavity flows down into the waste liquid box along the partition plate, waste liquid is collected, and the phenomenon that the culture dish is influenced by the pouring of the waste liquid is avoided; when the second cylinder pushes the piston rod to move forwards, the third connecting rod pushes the fourth connecting plate to rotate to a vertical state, the side wall of the fourth connecting plate is in contact with the inner wall of the fourth movable groove to provide supporting force for the fourth connecting plate, so that the fourth connecting plate cannot rotate continuously, the partition plate is in a horizontal state to seal the first through groove, and reagents can react in the reaction cavity; after the waste liquid box is placed into the cavity, the fixing groove is positioned below the fixing plate, the fixing plate is pushed into the fixing groove, so that the fixing plate is matched with the fixing groove, the waste liquid box is fixed, and the waste liquid box is prevented from being moved out from the space; the fixed plate seals the second groove after inserting in the fixed slot, prevents that the waste liquid in the waste liquid box from pouring out from the second groove, avoids chemical agent to reveal and causes the pollution to the environment.

The valve assembly comprises a circular ring 251 arranged on the inner wall of the bottle opening of the reagent bottle 25 and a third baffle 252 arranged on the circular ring 251, a third sliding block is arranged on the side wall of the third baffle 252, a third sliding groove matched with the third sliding block is arranged on the inner wall of the bottle opening of the reagent bottle 25, and a sealing spring is arranged on the third sliding block; the third baffle plate is matched with the circular ring, the circular ring is sealed under the action of the third baffle plate, the reagent bottle is sealed, and the reagent is prevented from being directly poured out of the reagent bottle; after the bearing plate is fixed on the side wall of the box body, the first cylinder drives the piston rod to extend out, the piston rod of the first cylinder penetrates through the circular ring to be in contact with the third baffle plate, the first cylinder pushes the third baffle plate to move upwards, so that a gap is formed between the third baffle plate and the circular ring, and the third baffle plate and the circular ring actually fall into the reaction cavity from the circular ring hole, so that carbon dioxide is provided for the first cavity to adjust the environment in the first cavity.

The structure of the oxygen generating device 23 is the same as that of the carbon dioxide generating device, the number of the reagent bottles can be set according to reactants, the oxygen generating device can adopt a hydrogen peroxide solution, and the carbon dioxide generating device can adopt sulfuric acid to react with baking soda; the power supply on the bearing plate is connected after the bearing plate is connected with the box body.

A third movable cavity communicated with the connecting groove is formed in the box body, a fourth connecting rod 13 penetrates through the third movable cavity, a fifth connecting plate is arranged on the first connecting block, a sixth connecting plate 131 matched with the fifth connecting plate is arranged on the fourth connecting rod, a fifth connecting rod 132 is arranged at the top of the fourth connecting rod, and the fifth connecting rod is arranged so as to be pulled to move upwards; when installing the board of accepting, upwards stimulate the fifth connecting rod, make the sixth connecting plate up move, insert first connecting block in the spread groove, down promote the fifth connecting rod afterwards, make sixth connecting plate and fifth connecting plate form the cooperation, it is fixed with first connecting block to accept the board and fix on the box lateral wall, accomplish the connection of accepting the board.

The bottom of the bearing plate is provided with a supporting plate 26, when the bearing plate is fixed on the side wall of the box body, the supporting plate, the bearing plate and the side wall of the box body form a triangular structure, a good supporting effect is provided for the bearing plate, the burden of the fifth connecting plate and the sixth connecting plate is reduced, and the use reliability of the bearing plate is ensured; the first connecting plate is provided with a third groove matched with the supporting plate, and the supporting plate and the third groove are matched to fix the bearing plate, so that the bearing plate is prevented from moving on the first connecting plate.

When a culture dish in one compartment needs to be taken out, the bearing plate is taken down from the first connecting plate, the first connecting block is aligned with the connecting groove, the fifth connecting rod is pulled upwards to drive the fourth connecting rod to move upwards, the first connecting block is inserted into the connecting groove, the fifth connecting plate is matched with the sixth connecting plate, the second connecting block is matched with the push plate, and the second single chip microcomputer controls the oxygen generating device and the carbon dioxide generating device to work to adjust the environment in the first through cavity; the second connecting block is pushed upwards, the second connecting block drives the first baffle to move upwards, the third connecting plate is pulled to enable the first connecting rod and the second connecting rod to rotate to a linear state, the first push rod is pushed to push the culture dish out of the compartment, the culture dish enters the first through cavity, the second push rod is pressed to enable the first baffle to seal the compartment again, and the second baffle is opened to take out the culture dish, so that embryos in a certain compartment can be observed independently.

Claims

1. A cattle and sheep embryo incubator comprises a box body (1) and a bearing plate (2), wherein a plurality of communicated separation bins are arranged on the box body (1), culture dishes (3) are arranged in the separation bins, a first movable cavity is arranged at the top of each separation bin, and a first baffle (11) is arranged in each first movable cavity; the method is characterized in that: be equipped with on box (1) lateral wall with accept corresponding first connecting plate (12) of board (2), accept be equipped with on board (2) with compartment matched with first logical chamber, it is equipped with oxygen generating device (23) and carbon dioxide generating device on board (2) to accept, first logical intracavity interpolation is equipped with second baffle (21), it is equipped with first connecting block (210) on board (2) to accept, be equipped with on box (1) lateral wall with first connecting block (210) matched with spread groove.

2. The cattle and sheep embryo incubator according to claim 1, wherein: be equipped with the second on the partition bin lateral wall and lead to the chamber, the second leads to and is equipped with first activity chamber on the intracavity wall, first push rod (14) are worn to be equipped with in the second leads to the chamber, be equipped with on first push rod (14) with first activity chamber matched with first limiting plate (141), be equipped with the extension bar subassembly on first push rod (14).

3. The cattle and sheep embryo incubator according to claim 2, wherein: be equipped with the second connecting plate on first push rod (14), the extension bar subassembly include rotatable coupling in head rod (15) on the second connecting plate, locate second connecting rod (16) on head rod (15) and locate third connecting plate (17) on second connecting rod (16), be equipped with first movable groove on head rod (15), second connecting rod (16) one end rotatable coupling in first movable groove one end, second connecting rod (16) other end swing joint in on third connecting plate (17), be equipped with on box (1) lateral wall with head rod (15) matched with first recess.

4. The cattle and sheep embryo incubator according to claim 1, wherein: be equipped with the second movable groove on the first movable cavity lateral wall, be equipped with on first baffle (11) with second movable groove matched with push pedal (111), be equipped with guide frame (28) on accepting board (2), be equipped with during guide frame (28) with push pedal (111) matched with second connecting block (29), be equipped with first slider on the second connecting block lateral wall, be equipped with on guide frame (28) inner wall with first slider matched with first spout.

5. The cattle and sheep embryo incubator according to claim 4, wherein: a third through cavity matched with the push plate (111) is arranged on the second connecting block (29), a second sliding groove is arranged on the side wall of the push plate (111), and a second sliding block matched with the second sliding groove is arranged on the inner wall of the third through cavity; and a first return spring (293) is arranged on the first sliding block.

6. The cattle and sheep embryo incubator according to claim 4, wherein: a third movable groove is formed in the side wall of the second connecting block (29), a limiting spring (291) is arranged in the third movable groove, a limiting block (292) is arranged at one end of the limiting spring (291), and a limiting groove matched with the limiting block (292) is formed in the inner wall of the guide frame (28); spacing groove one end is equipped with the fourth and leads to the chamber, be equipped with the second activity chamber on the fourth cross intracavity wall, second push rod (281) are worn to be equipped with in the fourth cross intracavity, be equipped with on second push rod (281) with second activity chamber matched with second limiting plate (2811), be equipped with second reset spring (2812) on second limiting plate (2811).

7. The cattle and sheep embryo incubator according to claim 1, wherein: carbon dioxide generating device includes brace table (22), locates reaction box (24) at brace table (22) top and locating reagent bottle (25) on reaction box (24), be equipped with the reaction chamber in reaction box (24), reaction box (24) top is equipped with go-between (242), reagent bottle (25) through threaded connection in on go-between (242), be equipped with the valve member on reagent bottle (25), be equipped with in the reaction chamber and be used for control first cylinder (245) that the valve member opened.

8. The cattle and sheep embryo incubator according to claim 7, wherein: be equipped with the cavity on brace table (22), wear to be equipped with waste liquid box (27) in the cavity, brace table (22) top be equipped with reaction chamber matched with first logical groove, rotatable coupling has baffle (221) on the first logical inslot wall, the cavity top is equipped with first mounting panel (224), be equipped with on first mounting panel (224) and be used for the drive second cylinder (225) of baffle (221) upset, be equipped with on waste liquid box (27) with second cylinder (225) matched with second recess.

9. The cattle and sheep embryo incubator according to claim 8, wherein: a fourth movable groove is formed in the bottom of the partition plate (221), a fourth connecting plate (222) is rotatably connected in the fourth movable groove, one side of the fourth connecting plate (222) is in contact with the inner wall of the fourth movable groove, a third connecting rod (223) is arranged on a piston rod of the second cylinder (225), and the third connecting rod (223) is movably connected to the fourth connecting plate (222); a fixing groove is formed in the third groove, a second through groove is formed in the top of the cavity, and a fixing plate (226) matched with the fixing groove penetrates through the second through groove.

10. The cattle and sheep embryo incubator according to claim 7, wherein: the valve component comprises a circular ring (251) arranged on the inner wall of the bottle opening of the reagent bottle (25) and a third baffle (252) arranged on the circular ring (251), a third sliding block is arranged on the side wall of the third baffle (252), a third sliding groove matched with the third sliding block is arranged on the inner wall of the bottle opening of the reagent bottle (25), and a sealing spring is arranged on the third sliding block.