CN110583710A - Multifunctional bacteriostatic composition and preparation method and application thereof - Google Patents
Multifunctional bacteriostatic composition and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a multifunctional bacteriostatic composition and a preparation method and application thereof, wherein the multifunctional bacteriostatic composition comprises stock solution or the stock solution and a diluent, and the stock solution is prepared from the following components in percentage by mass: 65-80% of natural camphor oil floral water, 1-10% of 1, 8-cineole, 1-2% of delta-terpineol, 0.5-1% of menthol, 0.01-0.05% of surfactant, 1-1.5% of camphor oil aqueous emulsion and the balance of water, wherein the obtained product is enhanced camphor oil floral water; the diluent is formed by mixing natural cinnamomum camphora floral water and water, and the mixing mass ratio is 4-7: 6-3. The multifunctional bacteriostatic composition has the advantages of simple preparation method, multiple functions of bacteriostasis, disinfection, cockroach avoidance and the like, no alcohol and wide application range.
Description
Technical Field
The invention relates to the field of daily chemicals, in particular to a multifunctional bacteriostatic composition and a preparation method and application thereof.
Background
Cinnamomum camphora, Lauraceae, is produced in Yibin city, Sichuan province, in China, with a yield of 70% of China, and is also called Yibin Cinnamomum camphora. The Yibin oil camphor root, branch, leaf and flower and fruit contain rich aromatic oil, and particularly the oil content of the leaf is as high as 3.8-4.5%. The camphor oil and the main components of 1, 8-cineole, alpha-terpineol (abienol) and sabinene have important application in the fields of national defense, light industry, perfume, medicine, advanced electroplating industry and the like. Yibin is used as the biggest cinnamomum camphora base in China, the annual cinnamomum camphora oil yield is approximately 10000 tons, and meanwhile, the waste natural cinnamomum camphora toilet water yield is approximately 100 ten thousand tons. At present, natural cinnamomum camphora floral water is treated by direct discharge, is not recycled and causes environmental pollution.
According to market investigation, most of the toilet water sold in the market at present contains alcohol, and the products are mainly used for preventing mosquito bites and relieving itching, are not suitable for people sensitive to alcohol, and can cause the problems of fire safety and the like; meanwhile, most cockroach killing agents sold in the market contain chemical components harmful to human bodies, such as permethrin, tetramethrin and the like, and the using steps are very complicated, and the required caution items are more (for example, people and animals should leave a room immediately after using the cockroach killing agent, doors and windows are closed, the doors and windows are opened after 20min, and the cockroach killing agent can enter the room after fully ventilating). The consumer has less choice in selecting a daily chemical product having multiple functions similar to the present invention.
Disclosure of Invention
The invention mainly solves the technical problem of providing a multifunctional bacteriostatic composition which has multiple functions of bacteriostasis, disinfection, cockroach avoidance and the like.
In order to solve the technical problems, the invention adopts a technical scheme that:
the antibacterial composition comprises a stock solution prepared from the following components in percentage by mass: 65-80% of natural camphor oil floral water, 1-10% of 1, 8-cineole, 1-2% of delta-terpineol, 0.5-1% of menthol, 0.01-0.05% of surfactant, 1-1.5% of camphor oil aqueous emulsion and the balance of water.
Further, the natural cinnamomum camphora floral water is a water-phase solution obtained after oil-water separation in the cinnamomum camphora oil steam distillation preparation process.
The extraction process of the natural cinnamomum camphora floral water comprises the following steps: in the process of preparing the cinnamomum longepaniculatum oil by steam distillation of cinnamomum longepaniculatum, when cinnamomum longepaniculatum volatile oil in cinnamomum longepaniculatum leaves is subjected to azeotropic distillation with water, the cinnamomum longepaniculatum volatile oil is carried out by the steam, enters a water-oil collector, is kept stand for more than 30min, is subjected to oil-water separation, and the obtained water phase is cinnamomum longepaniculatum floral water used in the invention.
Further, the surfactant is selected from one or more of tween 20, tween 80 and span 80.
Further, the cinnamomum longepaniculatum aqueous emulsion comprises cinnamomum longepaniculatum leaf volatile oil, an emulsifier, an antifreeze agent, a co-emulsifier and water;
the cinnamomum longepaniculatum aqueous emulsion contains 4% of cinnamomum longepaniculatum leaf volatile oil, 8% of emulsifier and 4% of antifreeze agent in percentage by weight; 0.2 percent of auxiliary emulsifier; 83.8 percent of water; the hydrophilic-lipophilic balance value of the emulsifier is 14;
the emulsifier is one or a mixture of any two of span and tween; the coemulsifier is one or a mixture of more of n-butanol, n-hexanol and n-octanol; the antifreezing agent is one or a mixture of more of ethanol, ethylene glycol and propylene glycol.
The span is sorbitan fatty acid ester, and the tween is polyoxyethylene sorbitan fatty acid ester.
The cinnamomum longepaniculatum aqueous emulsion used in the invention is prepared by the method in the prior art CN 103393754B.
Further, the bacteriostatic composition also comprises a diluent, wherein the diluent is prepared from natural cinnamomum camphora floral water: the water mass ratio is 4-7: 6-3.
Further, in the bacteriostatic composition of the invention, the diluent: the volume ratio of the stock solution is 0-19.
Further, the diluent: the volume ratio of the stock solution is 0-14.
In practical application, the bacteriostatic composition can also comprise 0.5-1.5 mass% of a flavoring agent.
In a specific embodiment of the invention, the flavoring agent is selected from one or more of rose, violet, cherry blossom, kiwi, freesia and jasmine essence.
The flavoring agent can be added and adjusted according to personal preference, and is not limited to the flavoring agent, and the flavoring agent existing in the field can be applied to the invention.
The invention also provides a preparation method of the antibacterial composition, which comprises the following steps: mixing the components in the stock solution, stirring, and mixing with other raw materials.
Further, the mixing sequence of the components in the stock solution is as follows: mixing menthol, 1, 8-cineole and delta-terpineol to obtain a mixture A, mixing the mixture A with a surfactant to obtain a mixture B, and finally mixing the mixture B with natural camphor oil toilet water, camphor oil emulsion in water and water.
In a specific embodiment of the present invention, after all the components in the stock solution are mixed, the mixture is stirred at a high speed to obtain the stock solution.
Further, the rotating speed of the high-speed stirring is 8000-12000 r/min.
The invention also provides application of the antibacterial composition in preparation of daily chemical articles.
Further, the daily chemical product is one or more of a skin disinfectant, a fragrance agent, an air disinfectant and a cockroach repellent.
The invention has the beneficial effects that:
(1) the multifunctional bacteriostatic composition has the functions of inhibiting and killing common pathogenic microorganisms such as escherichia coli and staphylococcus aureus on the skin, and can be used for preparing products such as skin disinfectants.
(2) The multifunctional bacteriostatic composition has a disinfecting effect on air, has an effect superior to 75% ethanol and 84 disinfectant, can be used as a novel air disinfectant, and is more healthy and environment-friendly.
(3) The multifunctional antibacterial composition has obvious cockroach repelling effect, can be used as a cockroach repelling agent, and makes up the defects of the products in the current market.
(4) The main components of the raw materials of the multifunctional bacteriostatic composition are natural components, do not contain chemical components harmful to human bodies, do not contain alcohol, are particularly suitable for alcohol-sensitive people, and can meet the disinfection requirement of the alcohol-sensitive people on the environment needing to be disinfected.
(5) The multifunctional bacteriostatic composition disclosed by the invention fully utilizes waste liquid (natural cinnamomum camphora floral water) in cinnamomum camphora oil production, reduces environmental pollution caused by waste water discharge in cinnamomum camphora volatile oil extraction, changes waste into valuable, reduces the cost, and is green and environment-friendly.
Drawings
FIG. 1 is an in vitro bacteriostasis test OD value;
FIG. 2 is the results of an air sterilization test;
FIG. 3 shows the results of the cockroach avoidance test.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The natural cinnamomum camphora floral water used in the embodiment of the invention is a water phase solution obtained by oil-water separation in the process of preparing cinnamomum camphora oil by steam distillation of cinnamomum camphora branches and leaves in the prior art, namely a wastewater liquid generated in the extraction of cinnamomum camphora oil by steam distillation.
The cinnamomum longepaniculatum leaf volatile oil aqueous emulsion used in the embodiment of the invention is cinnamomum longepaniculatum leaf volatile oil aqueous emulsion described in patent CN103393754B, and cinnamomum longepaniculatum leaf volatile oil aqueous emulsion within the protection range described in patent CN103393754B can be used in the invention, and is not limited to several types of cinnamomum longepaniculatum leaf volatile oil aqueous emulsions used in the embodiment.
In the embodiment of the invention, the used water is distilled water.
EXAMPLE 1 preparation of multifunctional bacteriostatic composition
The formula of the stock solution is as follows: the natural camphor oil aqueous emulsion comprises 6.5g of natural camphor oil aqueous emulsion, 0.1g of 1, 8-cineole, 0.1g of delta-terpineol, 0.05g of menthol, 200.001 g of tween, 0.1g of camphor oil aqueous emulsion and pure water, wherein the total amount of pure water is 10g, and the camphor oil aqueous emulsion is prepared in the patent CN103393754B in the embodiment 1.
(1) According to a stock solution formula table, respectively weighing menthol, 1, 8-eucalyptol and delta-terpineol in a 100mL beaker;
(2) finely weighing Tween-20, placing in the beaker in the step (1), fully shaking and standing;
(3) accurately weighing natural camphor oil toilet water, camphor oil emulsion in water and pure water in a stock solution formula, and adding the natural camphor oil toilet water, the camphor oil emulsion in water and the pure water into a beaker;
(4) stirring the mixture obtained in the step (3) for 1min by a high-speed stirrer at 8000r/min to obtain milky white liquid, namely stock solution;
(5) diluting the stock solution obtained in the step (4) by using a diluent (the mass of the natural cinnamomum camphora floral water: the mass of pure water: 7:3) with the volume of 4 times, and diluting to obtain a diluent with the volume of 5 times, so as to obtain the multifunctional bacteriostatic composition, namely the enhanced cinnamomum camphora floral water.
EXAMPLE 2 preparation of multifunctional bacteriostatic composition
The formula of the stock solution is as follows: 8.0g of natural camphor oil toilet water, 1.0g of 1, 8-cineole, 0.2g of delta-terpineol, 0.1g of menthol, 800.005 g of tween, 0.15g of camphor oil aqueous emulsion and pure water, wherein the camphor oil aqueous emulsion is prepared from the camphor oil aqueous emulsion prepared in the embodiment 4 in the patent CN103393754B until the total amount is 10 g.
(1) According to a stock solution formula table, respectively weighing menthol, 1, 8-eucalyptol and delta-terpineol in a 100mL beaker;
(2) finely weighing Tween 80, placing in the beaker in the step (1), fully shaking and standing;
(3) accurately weighing natural camphor oil toilet water, camphor oil emulsion in water and pure water in a stock solution formula, and adding the natural camphor oil toilet water, the camphor oil emulsion in water and the pure water into a beaker;
(4) stirring the mixture obtained in the step (3) for 1min by a high-speed stirrer at 12000r/min to obtain milky white liquid, namely stock solution;
(5) diluting the stock solution obtained in the step (4) by using a diluent (the mass of the natural cinnamomum camphora floral water: the mass of pure water: 5) with the volume of 14 times, and diluting to obtain a 15-time diluent, so as to obtain the multifunctional bacteriostatic composition, namely the enhanced cinnamomum camphora floral water.
EXAMPLE 3 preparation of multifunctional bacteriostatic composition
The formula of the stock solution is as follows: 7.5g of natural camphor oil toilet water, 0.5g of 1, 8-cineole, 0.1g of delta-terpineol, 0.05g of menthol, 800.003 g of span, 0.12g of camphor oil aqueous emulsion and pure water, wherein the camphor oil aqueous emulsion is prepared by the camphor oil aqueous emulsion prepared in the embodiment 2 in the patent CN103393754B until the total amount is 10 g.
(1) According to a stock solution formula table, respectively weighing menthol, 1, 8-eucalyptol and delta-terpineol in a 100mL beaker;
(2) placing the fine-weighed span 80 in the beaker in the step (1), fully vibrating and standing;
(3) accurately weighing natural camphor oil toilet water, camphor oil emulsion in water and pure water in a stock solution formula, and adding the natural camphor oil toilet water, the camphor oil emulsion in water and the pure water into a beaker;
(4) stirring the mixture obtained in the step (3) for 1min by a high-speed stirrer at 10000r/min to obtain milky white liquid, namely stock solution;
(5) diluting the stock solution obtained in the step (4) by using 9 times of diluent (the mass of the natural cinnamomum camphora floral water: the mass of pure water is 4:6), and diluting to obtain 10 times of diluent to obtain the multifunctional bacteriostatic composition, namely the enhanced cinnamomum camphora floral water.
EXAMPLE 4 preparation of multifunctional bacteriostatic composition
The formula of the stock solution is as follows: 7.5g of natural camphor oil toilet water, 0.5g of 1, 8-cineole, 0.1g of delta-terpineol, 0.05g of menthol, 200.003 g of tween, 0.12g of camphor oil aqueous emulsion and pure water till the total amount is 10g, wherein the camphor oil aqueous emulsion is prepared by the camphor oil aqueous emulsion prepared in the embodiment 3 in the patent CN 103393754B.
(1) According to a stock solution formula table, respectively weighing menthol, 1, 8-eucalyptol and delta-terpineol in a 100mL beaker;
(2) finely weighing Tween 20, placing in the beaker in the step (1), fully shaking and standing;
(3) accurately weighing natural camphor oil toilet water, camphor oil emulsion in water and pure water in a stock solution formula, and adding the natural camphor oil toilet water, the camphor oil emulsion in water and the pure water into a beaker;
(4) stirring the mixture obtained in the step (3) for 1min by a high-speed stirrer at 12000r/min to obtain milky white liquid, namely stock solution;
(5) diluting the stock solution obtained in the step (4) by using a diluent (the mass of the natural cinnamomum camphora floral water: the mass of pure water: 6:4) with the volume of 4 times, and diluting to obtain a diluent with the volume of 5 times, so as to obtain the multifunctional bacteriostatic composition, namely the enhanced cinnamomum camphora floral water.
The beneficial effects of the multifunctional bacteriostatic composition are verified by the following test examples:
the multifunctional bacteriostatic composition (enhanced camphor floral water) used in the experimental examples was prepared by mixing the stock solutions of the formulations in example 4, and the enhanced camphor floral water used in the experimental examples was prepared by mixing the stock solution and the diluent of the formulations in example 4, namely 5-fold diluent, 10-fold diluent and 15-fold diluent.
Test example 1 in vitro bacteriostatic test
The multifunctional bacteriostatic composition (enhanced camphor floral water, namely the stock solution of the composition, 5-time diluent, 10-time diluent and 15-time diluent) is selected as a research object, natural camphor floral water and 1, 8-cineole solution are used as negative controls, commercially available Liushen floral water is used as a positive control, distilled water is used as a blank control, the multifunctional bacteriostatic composition is respectively cultured with mixed liquid of escherichia coli and staphylococcus aureus for 8 hours, and the OD value is determined, and the result is shown in table 1 and figure 1. The concentration of the 1, 8-cineole in the 1, 8-cineole solution is the same as that of the 1, 8-cineole in the enhanced camphor flower dew used in the test, and the solvent is the natural camphor flower dew: adding Tween 20 with the mass ratio of 1% into the diluent with the mass ratio of 6: 4.
TABLE 1 OD value of in vitro bacteriostasis test (incubation with mixed liquid of Escherichia coli and Staphylococcus aureus for 8h)
The results show that the multifunctional bacteriostatic composition (the enhanced camphor floral water is the original liquid of the composition, the 5-time diluent, the 10-time diluent and the 15-time diluent of the enhanced camphor floral water) has bacteriostatic effects, and the bacteriostatic effects are gradually weakened along with the reduction of the concentration. Wherein, the bacteriostatic effect of the enhanced camphor wood floral water 10 times of the diluent is equivalent to that of Liushen floral water, and the bacteriostatic effect of the enhanced camphor wood floral water 15 times of the diluent is equivalent to that of natural camphor wood floral water.
Test example 2 air sterilization test
The multifunctional bacteriostatic composition (enhanced camphor floral water, namely the stock solution of the composition, 5-time diluent, 10-time diluent and 15-time diluent) is selected as a research object, 75% ethanol and 84 disinfectant are used as positive controls, distilled water is used as a blank control, a flat plate sampling sedimentation method is adopted for sampling for carrying out an air disinfection test, and the result is shown in figure 2.
The results show that the multifunctional bacteriostatic composition (the enhanced camphor floral water is the original solution of the composition, the 5-time diluent, the 10-time diluent and the 15-time diluent of the enhanced camphor floral water) has air disinfection effect, and the air disinfection effect is gradually weakened along with the reduction of the concentration. Wherein, the air disinfection effect of the 5 times of the enhanced cinnamomum camphora floral water diluent is equivalent to the disinfection effect of 75% ethanol and 84 disinfectant, and the bacteriostatic effect of the 15 times of the enhanced cinnamomum camphora floral water diluent is equivalent to the air disinfection effect of natural cinnamomum camphora floral water.
Test example 3 cockroach avoidance test
The multifunctional bacteriostatic composition (enhanced camphor floral water, namely the stock solution of the composition, 5-time diluent, 10-time diluent and 15-time diluent of the enhanced camphor floral water) is selected as a research object, distilled water is used as a blank control, most of products for removing cockroaches on the market are used for killing the cockroaches after being lured, and the cockroaches removing agent with aversion is very little, so a positive control is not designed in the experiment, and the experiment is carried out in the following way: the storage box is cleaned and placed in an air circulation position to be dried, so that the storage box is odorless. 20 cockroaches with the same size and physiological state, such as 2.5cm in length and the like are placed in a storage box, and a storage cover is placed on the storage box. 1g of absorbent cotton is finely weighed, then 3g of distilled water (blank control), enhanced oil camphor floral water, 5-fold diluent, 10-fold diluent and 15-fold water diluent are respectively weighed and uniformly sprayed on the absorbent cotton, the absorbent cotton is placed in a culture dish, and finally the culture dish is respectively placed in the middle of a storage box. Standing for 10 minutes, recording the physiological state of the cockroaches in the storage box and the number of the cockroaches, and calculating the avoidance rate. The test results are shown in FIG. 3.
The results show that the multifunctional bacteriostatic composition (the enhanced camphor floral water is the original liquid of the composition, the 5-time diluent, the 10-time diluent and the 15-time diluent of the enhanced camphor floral water) has obvious avoidance effect on cockroaches, and the avoidance effect on the cockroaches is gradually weakened along with the reduction of the concentration. Wherein, the bacteriostatic effect of the 15 times of the diluent of the enhanced cinnamomum camphora floral water is equivalent to the repellent effect of natural cinnamomum camphora floral water on cockroaches.
Test example 4 perfuming test
In order to enrich the aroma types of the floral water products, 6 essences of rose aroma, violet aroma, cherry aroma, kiwi fruit, litsea cubeba and jasmine are respectively added into the antibacterial composition to meet the aroma preference of more consumers, and the addition amount of the essence is 0.5-1.5% of the mass of the antibacterial combined group. Tests show that the fragrance of 6 perfumed products is superior to that of unflavored cinnamomum camphora toilet water, and the perfumed products do not influence the biological activity of the perfumed products.
Of course, other flavors in the art can be used as the flavoring agent in the antibacterial composition of the present invention, and are not limited to the above 6 flavors.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. The bacteriostatic composition is characterized by comprising a stock solution prepared from the following components in percentage by mass: 65-80% of natural camphor oil floral water, 1-10% of 1, 8-cineole, 1-2% of delta-terpineol, 0.5-1% of menthol, 0.01-0.05% of surfactant, 1-1.5% of camphor oil aqueous emulsion and the balance of water.
2. The bacteriostatic composition according to claim 1, wherein the natural cinnamomum camphora floral water is an aqueous solution obtained after oil-water separation during cinnamomum camphora steam distillation for preparing cinnamomum camphora oil.
3. The bacteriostatic composition according to claim 1, wherein the surfactant is selected from one or more of tween 20, tween 80 and span 80; further, the surfactant is selected from tween 20.
4. The bacteriostatic composition according to claim 1, wherein the cinnamomum longepaniculatum aqueous emulsion comprises cinnamomum longepaniculatum leaf volatile oil, an emulsifier, an antifreeze agent, a co-emulsifier and water;
the cinnamomum longepaniculatum aqueous emulsion contains 4% of cinnamomum longepaniculatum leaf volatile oil, 8% of emulsifier and 4% of antifreeze agent in percentage by weight; 0.2 percent of auxiliary emulsifier; 83.8 percent of water; the hydrophilic-lipophilic balance value of the emulsifier is 14;
the emulsifier is one or a mixture of any two of span and tween; the coemulsifier is one or a mixture of more of n-butanol, n-hexanol and n-octanol; the antifreezing agent is one or a mixture of more of ethanol, ethylene glycol and propylene glycol.
5. The bacteriostatic composition according to claim 1, further comprising a diluent consisting of natural cinnamomum camphora floral water: the water mass ratio is 4-7: 6-3.
6. Bacteriostatic composition according to claim 5, wherein the diluent: the volume ratio of the stock solution is 0-19; further, the diluent: the volume ratio of the stock solution is 0-14.
7. A bacteriostatic composition according to any one of claims 1 to 6, which further comprises 0.5 to 1.5% of a flavoring agent; further, the flavoring agent is selected from one or more of rose essence, violet essence, cherry essence, kiwi fruit essence, freesia, and jasmine essence.
8. A preparation method of the bacteriostatic composition according to any one of claims 1 to 7, characterized by comprising the following steps: mixing and stirring the components in the stock solution, and then mixing the mixture with other raw materials to obtain the compound feed; further, the mixing sequence of the components in the stock solution is as follows: mixing menthol, 1, 8-cineole and delta-terpineol to obtain a mixture A, mixing the mixture A with a surfactant to obtain a mixture B, and finally mixing the mixture B with natural camphor oil toilet water, camphor oil emulsion in water and water.
9. The method according to claim 7, wherein after all the components in the stock solution are mixed, the mixture is stirred at a high speed to obtain the stock solution; further, the rotating speed of the high-speed stirring is 8000-12000 r/min.
10. Use of the bacteriostatic composition according to any one of claims 1 to 7 in the preparation of daily chemical products; further, the daily chemical product is one or more of a skin disinfectant, a fragrance agent, an air disinfectant and a cockroach repellent.
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