CN110582566B - 肽连接酶及其用途 - Google Patents

肽连接酶及其用途 Download PDF

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Publication number
CN110582566B
CN110582566B CN201880024247.3A CN201880024247A CN110582566B CN 110582566 B CN110582566 B CN 110582566B CN 201880024247 A CN201880024247 A CN 201880024247A CN 110582566 B CN110582566 B CN 110582566B
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Prior art keywords
peptide
tag
polypeptide
seq
ligase
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CN201880024247.3A
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English (en)
Chinese (zh)
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CN110582566A (zh
Inventor
M·豪沃思
C·M·布敦
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Oxford University Innovation Ltd
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Oxford University Innovation Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3156—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci from Streptococcus pneumoniae (Pneumococcus)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pulmonology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201880024247.3A 2017-04-10 2018-04-10 肽连接酶及其用途 Active CN110582566B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202410483896.7A CN118389457A (zh) 2017-04-10 2018-04-10 肽连接酶及其用途

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1705750.6 2017-04-10
GBGB1705750.6A GB201705750D0 (en) 2017-04-10 2017-04-10 Peptide ligase and use therof
PCT/GB2018/050943 WO2018189517A1 (en) 2017-04-10 2018-04-10 Peptide ligase and use thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202410483896.7A Division CN118389457A (zh) 2017-04-10 2018-04-10 肽连接酶及其用途

Publications (2)

Publication Number Publication Date
CN110582566A CN110582566A (zh) 2019-12-17
CN110582566B true CN110582566B (zh) 2024-05-10

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CN201880024247.3A Active CN110582566B (zh) 2017-04-10 2018-04-10 肽连接酶及其用途
CN202410483896.7A Pending CN118389457A (zh) 2017-04-10 2018-04-10 肽连接酶及其用途

Family Applications After (1)

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CN202410483896.7A Pending CN118389457A (zh) 2017-04-10 2018-04-10 肽连接酶及其用途

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US (1) US10889622B2 (enExample)
EP (1) EP3610005B1 (enExample)
JP (1) JP7075678B2 (enExample)
KR (1) KR102669378B1 (enExample)
CN (2) CN110582566B (enExample)
AU (1) AU2018251237B2 (enExample)
BR (1) BR112019021009A2 (enExample)
CA (1) CA3059132A1 (enExample)
DK (1) DK3610005T3 (enExample)
ES (1) ES2900612T3 (enExample)
GB (1) GB201705750D0 (enExample)
WO (1) WO2018189517A1 (enExample)

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US11453883B2 (en) 2018-04-05 2022-09-27 Bio-Rad Abd Serotec Gmbh Display systems for proteins of interest
BR112020022393A2 (pt) 2018-05-04 2021-02-02 SpyBiotech Limited composição, vacina compreendendo uma composição, método para produzir uma composição, molécula de ácido nucleico, e, vetor, célula hospedeira, kit compreendendo uma composição, e, vacinas para uso na profilaxia e/ou tratamento de infecção por citomegalovírus e por vírus sincicial respiratório.
GB201819850D0 (en) 2018-12-05 2019-01-23 Univ Oxford Innovation Ltd Polypeptide and its use in affinity purification
GB201903479D0 (en) 2019-03-14 2019-05-01 Univ Oxford Innovation Ltd Polypeptide with enchanced rate of spontaneous isopeptide bond formation with it's peptide tag partner and uses thereof
CN113874518B (zh) 2019-03-18 2025-04-25 生物辐射Abd瑟罗泰克有限公司 保护含SpyTag的周质融合蛋白免于蛋白酶Tsp和OmpT降解
GB201915905D0 (en) 2019-11-01 2019-12-18 Spybiotech Ltd Viruses with modified capsid proteins
US12298314B2 (en) 2020-10-09 2025-05-13 10X Genomics, Inc. Methods and compositions for analyzing target binding of molecules
WO2022076914A1 (en) 2020-10-09 2022-04-14 10X Genomics, Inc. Methods and compositions for profiling immune repertoire
GB202019817D0 (en) 2020-12-15 2021-01-27 Univ Oxford Innovation Ltd Ligand-binding polypeptides and uses thereof
CN112877347B (zh) * 2021-01-28 2023-02-17 华南理工大学 一种多酶复合体及其构建方法和应用
GB202104104D0 (en) 2021-03-24 2021-05-05 Liliumx Ltd Platform and method
GB202104999D0 (en) 2021-04-08 2021-05-26 Univ Oxford Innovation Ltd Polypeptides that interact with peptide tags at loops or termini and uses thereof
EP4333864A1 (en) 2021-05-04 2024-03-13 Spybiotech Limited Adenoviral vectors and vaccines thereof
GB202106361D0 (en) 2021-05-04 2021-06-16 Spybiotech Ltd Viral vectored vaccines
GB202117283D0 (en) 2021-11-30 2022-01-12 Univ Oxford Innovation Ltd Switchable polypeptide and its use for gentle affinity purification
EP4206674B1 (en) 2021-12-28 2025-10-22 Encodia, Inc. High-throughput serotyping and antibody profiling assays
KR20250131767A (ko) 2022-09-28 2025-09-03 발린크 테라퓨틱스 리미티드 다가 단백질 및 스크리닝 방법
GB202306808D0 (en) 2023-05-09 2023-06-21 Univ Oxford Innovation Ltd Method for detecting interactions
GB202313175D0 (en) 2023-08-30 2023-10-11 Univ Oxford Innovation Ltd A bivalent polypeptide scaffold and uses thereof
WO2025054526A1 (en) 2023-09-07 2025-03-13 Regeneron Pharmaceuticals, Inc. Production and purification of covalently surface modified adeno-associated virus
WO2025083285A1 (en) 2023-10-20 2025-04-24 Norwegian University Of Science And Technology (Ntnu) Methods and products for use in genome-wide chromatin profiling
WO2025111473A1 (en) 2023-11-21 2025-05-30 Regeneron Pharmaceuticals, Inc. Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus
GB202317925D0 (en) 2023-11-23 2024-01-10 Spybiotech Ltd Vaccines

Citations (3)

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CN1268976A (zh) * 1997-05-16 2000-10-04 诺沃诺尔迪斯克生物技术有限公司 具有脯氨酰二肽氨肽酶活性的多肽和编码该多肽的核酸
CN106414733A (zh) * 2014-06-03 2017-02-15 帝斯曼知识产权资产管理有限公司 脯氨酸特异性内切蛋白酶及其用途
CN106459948A (zh) * 2014-06-03 2017-02-22 帝斯曼知识产权资产管理有限公司 脯氨酸特异性内切蛋白酶及其用途

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GB0714963D0 (en) 2007-08-01 2007-09-12 Novartis Ag Compositions comprising antigens
NZ594029A (en) 2009-01-12 2014-01-31 Novartis Ag Cna_b domain antigens in vaccines against gram positive bacteria
GB201002362D0 (en) * 2010-02-11 2010-03-31 Isis Innovation Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds
EP2817320A1 (en) * 2012-02-24 2014-12-31 Novartis AG Pilus proteins and compositions
EA035378B1 (ru) 2015-01-15 2020-06-04 Юниверсити Оф Копенгаген Вирусоподобная частица с эффективным экспонированием эпитопов
GB201509782D0 (en) * 2015-06-05 2015-07-22 Isis Innovation Methods and products for fusion protein synthesis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1268976A (zh) * 1997-05-16 2000-10-04 诺沃诺尔迪斯克生物技术有限公司 具有脯氨酰二肽氨肽酶活性的多肽和编码该多肽的核酸
CN106414733A (zh) * 2014-06-03 2017-02-15 帝斯曼知识产权资产管理有限公司 脯氨酸特异性内切蛋白酶及其用途
CN106459948A (zh) * 2014-06-03 2017-02-22 帝斯曼知识产权资产管理有限公司 脯氨酸特异性内切蛋白酶及其用途

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Publication number Publication date
CA3059132A1 (en) 2018-10-18
DK3610005T3 (da) 2021-12-13
JP7075678B2 (ja) 2022-05-26
US20200115422A1 (en) 2020-04-16
ES2900612T3 (es) 2022-03-17
KR20190137820A (ko) 2019-12-11
AU2018251237B2 (en) 2024-01-18
CN118389457A (zh) 2024-07-26
KR102669378B1 (ko) 2024-05-24
US10889622B2 (en) 2021-01-12
AU2018251237A1 (en) 2019-10-24
CN110582566A (zh) 2019-12-17
EP3610005A1 (en) 2020-02-19
GB201705750D0 (en) 2017-05-24
JP2020516314A (ja) 2020-06-11
WO2018189517A1 (en) 2018-10-18
BR112019021009A2 (pt) 2020-05-05
EP3610005B1 (en) 2021-09-29

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